Observation device based on toxicity test and method thereof

Abstract

The invention discloses an observation device based on toxicity test and a method thereof, and belongs to the technical field of observation devices. A plurality of long-strip-shaped observation boxes are mounted on the base station; the observation box is provided with a detection mechanism for detecting the weight and the movement ability of the mouse; the detection mechanism comprises a floating type feeding table, an adjusting supporting assembly and an electronic scale. One end of the observation box is provided with a floating type feeding table for feeding, and the other end of the observation box is provided with a floating type feeding table for feeding water; adjusting supporting assemblies used for adjusting the height of the floating type feeding table are installed on the lower sides of the two ends of the observation box. An electronic scale used for weighing the floating type feeding table is arranged at the top of the adjusting supporting assembly; a collecting mechanism for collecting and storing the excrement of the mice in the observation boxes according to the defecation time and sequence is mounted on the lower side, aligned to the observation boxes, of the base station. By means of the mode, the excrement of the mouse is automatically collected and stored in real time according to time in the observation process.

Description

Technical Field

[0001] The present invention relates to the technical field of observation devices, and in particular to an observation device and a method thereof based on toxicity testing. Background Art

[0002] The basic operation of animal toxicity experiments is to inject toxic drugs into mice by injection, feeding or gavage, and then place the mice in an observation device. The experimenters observe, record and analyze the mouse's physical signs such as weight, mobility, drug residues in feces, as well as the overall mortality rate and the drug injection dosage corresponding to death.

[0003] For example, Chinese patent CN219330352U discloses a mouse observation box and mouse experimental equipment, which are provided with an observation window, a feces storage mechanism and a cleaning mechanism. The condition of the mice is observed through the observation window, and the mouse feces is swept into the feces storage mechanism for collection and storage through the cleaning mechanism.

[0004] However, the time and frequency of mouse defecation are difficult to control, and the feces will easily dry out if not preserved in time after excretion, or multiple excrements will accumulate, making it difficult to record the excretion time corresponding to the feces, affecting the accuracy of the test; and when the device is used to test the weight of mice, the mice need to be taken out of the observation box for measurement, which is inconvenient to operate.

[0005] Based on this, the present invention designs an observation device based on toxicity testing and a method thereof to solve the above problems. Summary of the invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides an observation device and method based on toxicity testing.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A toxicity test-based observation device includes a base station;

[0009] A plurality of long strip observation boxes are installed on the base platform; a detection mechanism for detecting the weight and exercise ability of mice is installed on the observation box;

[0010] The detection mechanism includes a floating feeding platform, an adjustment support assembly and an electronic scale; a floating feeding platform for feeding is installed at one end of the observation box, and a floating feeding platform for feeding water is installed at the other end of the observation box; an adjustment support assembly for adjusting the height of the floating feeding platform is installed on the lower side of both ends of the observation box; an electronic scale for weighing the floating feeding platform is arranged on the top of the adjustment support assembly;

[0011] A collecting mechanism for collecting and storing mouse feces in a plurality of observation boxes according to the defecation time and sequence is installed on the base aligned with the lower side of the observation box.

[0012] Furthermore, the observation box includes an observation box body, a locking assembly and a blocking assembly. The observation box body is installed on a base, the locking assembly is installed on the observation box body, and the blocking assembly is installed on the observation box body.

[0013] Furthermore, the main body of the observation box includes a first support frame, a first support platform, a box body, a side cover, a feeding port, a middle cover and a bottom structure, the two ends of the box body are respectively fixedly mounted on the upper side of a first support platform, the first support platform is fixedly mounted on the upper side of the first support frame, and the first support frame is fixedly mounted on the upper side of the base; a middle cover is rotatably mounted in the middle of the top of the box body, two side covers are symmetrically arranged at the two ends of the box body, one side of the side cover is rotatably connected to the top of the box body, and a feeding port is opened on the side of the side cover; a bottom structure is installed at the bottom of the box body; a floating feeding platform is symmetrically mounted at the two ends of the bottom of the box body; a moving space for mice is formed between the box body, the side covers, the middle cover, the bottom structure and the floating feeding platform; locking components for locking the side covers are installed on the box body and the side covers, and a locking component for locking the middle cover is installed between the box body and the middle cover.

[0014] Furthermore, the bottom structure includes a collecting bucket and round rods, and a plurality of crisscrossing round rods are fixedly installed on the bottom of the box body; and a collecting bucket that is larger at the top and smaller at the bottom is fixedly installed on the bottom of the box body.

[0015] Furthermore, the locking assembly includes a locking hole and a locking pin, and a locking hole is opened on the side of the box body, side cover and middle cover away from the hinge point; the locking hole on the box body is aligned with the locking holes on the side cover and the middle cover; the locking pin is plugged into the locking hole.

[0016] Furthermore, the blocking assembly includes a mounting block, a plug rod and a slot. The plug rods are evenly fixedly installed on the mounting block at equal intervals. The sides of the box body are symmetrically provided with through slots, which are located between the side cover and the middle cover; the plug rods are plugged into the slots.

[0017] Furthermore, the floating feeding platform includes a slide bar, a limit block, a support plate, a feeding trough and a baffle. The support plate is located in a box body, and a plurality of slide bars are symmetrically fixedly installed on the lower side of the support plate. The bottom of the slide bar passes through the lower side of the box body and is fixedly connected to the limit block; the slide bar is limitedly slidably connected to the box body; a feeding trough is fixedly installed on the upper side of the outer end of the support plate, and the feeding trough is aligned with the feeding port; and a baffle is fixedly installed on the lower side of the inner end of the support plate.

[0018] Furthermore, the adjustment support assembly includes a first motor, a screw, a movable sleeve rod, a guide rod and a second support platform. The first motor is fixedly mounted on the lower side of the first support frame, and the output end of the first motor passes through the first support frame and the first support platform and is fixedly connected to the lower end of the screw. The lower side of the movable sleeve rod is sleeved outside the screw, and the screw is threadedly connected to the movable sleeve rod through a threaded sleeve; a second support platform is fixedly mounted on the top of the movable sleeve rod, and a guide rod is symmetrically fixedly mounted on the lower side of the second support platform, and the guide rod is limitedly slidably connected to the first support platform; an electronic scale for weighing the support plate is fixedly mounted on the upper side of the second support platform.

[0019] Furthermore, the collecting mechanism includes a film, a feeding roller, a guide roller, an adjusting roller, a feeding roller, a winding roller and a second support frame, the second support frame is symmetrically fixedly installed on the base, and the second support frame is rotatably installed from left to right in sequence with a feeding roller for driving the film to unwind, a guide roller for guiding and supporting the film, an adjusting roller for adjusting the film tension, a feeding roller for driving the film to move to the right at a uniform speed and a winding roller for winding the film; the film between the feeding roller and the winding roller is aligned with the lower side of multiple collecting buckets.

[0020] In order to better achieve the purpose of the present invention, the present invention also provides a test method of an observation device based on toxicity testing, comprising the following steps:

[0021] Step 1: Select a number of mice and divide them into three groups: a, b and c; during the experiment, the mice are administrated orally or intragastrically;

[0022] Step 2: administering drugs to mice in group A, and increasing the dosage after observing the recovery of the mice, and administering drugs again, and repeating this process for multiple times until the dosage of mice in group A is close to the lethal dose range, so as to cultivate drug resistance in mice; mice in groups B and C are not given drugs in the initial state; placing the mice in the main body of the observation box, closing the main body of the observation box and locking it through the locking component, and monitoring the physical signs of the mice in the main body of the observation box;

[0023] Step 3: When the mouse leaves the floating feeding table at one end, the electronic scale on the lower side of the floating feeding table is reset to zero. When the mouse returns to the floating feeding table, the weight of the mouse on the floating feeding table is weighed by the electronic scale;

[0024] Step 4: After the mice are dosed, the floating feeding platforms at both ends are adjusted to a lower position by adjusting the support components so that the mice can normally move to the floating feeding platform to eat. In the subsequent feeding process, the height of the floating feeding platform is gradually raised by adjusting the support components. If the mice cannot or have difficulty moving to the floating feeding platform after raising the height of the floating feeding platform, the height of the floating feeding platform can be lowered; until the mice can normally climb to the floating feeding platform at a higher position, it is determined that the mice have recovered their mobility;

[0025] Step 5: While carrying out steps 1, 2 and 3, the mouse feces are collected and stored in real time by the collection mechanism, and the time of each defecation of the mouse can be recorded by the collection mechanism, so that the experimenter does not need to collect the mouse feces in real time and record the defecation time; the record is more accurate, the collection and storage are more timely, and the accuracy of the feces sample is guaranteed to the maximum extent; by testing the components of the mouse feces sample, it is determined whether there are toxic drug residues and the residual amount in the mouse feces, as well as the change of the toxic drug residue in the mouse feces over time;

[0026] Step 6: Repeat steps 2, 3, 4 and 5 until the dosage of mice in group A is close to the lethal dosage range, and increase the dosage to mice in groups A and B at the same time; observe the status and mortality of mice in groups A and B, and draw corresponding experimental conclusions;

[0027] Step 7: All surviving mice are assigned to group a, and mice from group c are selected to group b; after a period of feeding, when the mice recover, the dosage is increased again and administered to the mice in groups a and b; the status and mortality of the mice in groups a and b are observed, and corresponding experimental conclusions are drawn; and this operation is repeated until the mortality of one group of mice in groups a and b is greater than 90%, and the experiment is terminated.

[0028] Compared with the prior art, the present invention has the following beneficial effects: 1. By arranging the floating feeding platforms for feeding food and water at the two ends of the main body of the observation box, the mice are prevented from staying at the same end continuously; when the mice leave the floating feeding platform at one end, the electronic scale on the lower side of the floating feeding platform is reset to zero, and when the mice return to the floating feeding platform, the weight of the mice on the floating feeding platform is weighed by the electronic scale;

[0029] 2. After the mice are given the drug, the floating feeding platforms at both ends are adjusted to a lower position by adjusting the support components so that the mice can normally move to the floating feeding platform to eat. In the subsequent feeding process, the height of the floating feeding platform is gradually raised by adjusting the support components. If the mice cannot or find it difficult to move to the floating feeding platform after raising the height of the floating feeding platform, the height of the floating feeding platform can be lowered; until the mice can normally climb to the floating feeding platform at a higher position, then it is determined that the mice have recovered their mobility;

[0030] 3. The arrangement of the observation box body, the locking component and the blocking component facilitates the removal of mice from the observation box body, thereby preventing the mice from escaping from the observation box body when the mice are removed;

[0031] 4. The feces produced by mice are collected and preserved in real time through the collection mechanism. At the same time, the collection mechanism can record the time of each defecation of mice, so that the experimenter does not need to collect the mouse feces in real time and record the defecation time; making the record more accurate, the collection and preservation more timely, and maximizing the accuracy of fecal samples; by testing the components of mouse fecal samples, it is possible to determine whether there are toxic drug residues and the residual amount in the mouse feces, as well as the changes in the toxic drug residues in the mouse feces over time. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0033] Figure 1 A three-dimensional observation device based on toxicity testing of the present invention Figure 1 ;

[0034] Figure 2 A front view of an observation device based on toxicity testing of the present invention;

[0035] Figure 3 is a schematic diagram of the first support platform and its connection structure;

[0036] Figure 4 Schematic diagram of the box and its connection structure;

[0037] Figure 5 is a schematic diagram of the side cover and its connection structure;

[0038] Figure 6 It is a schematic diagram of the feeding trough and its connection structure;

[0039] Figure 7 is a schematic diagram of the first support platform and its connection structure;

[0040] Figure 8 is a schematic diagram of a second support frame and its connection structure;

[0041] Fig. 9 Schematic diagram of the film and its connection structure.

[0042] The numbers in the figure represent:

[0043] 1. Base; 2. Observation box; 21. Observation box body; 211. First support frame; 212. First support platform; 213. Box body; 214. Side cover; 215. Feeding port; 216. Middle cover; 217. Collection bucket; 218. Round rod; 22. Locking assembly; 221. Locking hole; 222. Locking pin; 23. Blocking assembly; 231. Mounting block; 232. Inserting rod; 3. Detection mechanism; 31. Floating feeding platform; 31 1. Slide bar; 312. Limit block; 313. Support plate; 314. Feeding trough; 315. Baffle; 32. Adjusting support assembly; 321. First motor; 322. Screw rod; 323. Moving sleeve rod; 324. Guide rod; 325. Second support platform; 33. Electronic scale; 4. Collecting mechanism; 41. Film; 42. Feeding roller; 43. Guide roller; 44. Adjusting roller; 45. Feeding roller; 46. Winding roller; 47. Second support frame. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] The terms “left”, “right”, “front”, “back”, “up” and “down” mentioned in the following description are oriented in the viewing direction of the front view.

[0046] Embodiment 1: In some embodiments, please refer to the drawings of the specification Figure 1-Figure 3 , an observation device based on toxicity testing, comprising a base station 1;

[0047] A plurality of long strip-shaped observation boxes 2 are installed on the base platform 1; a detection mechanism 3 for detecting the weight and exercise ability of mice is installed on the observation box 2;

[0048] The detection mechanism 3 includes a floating feeding table 31, an adjustment support assembly 32 and an electronic scale 33; a floating feeding table 31 for feeding is installed at one end of the observation box 2, and a floating feeding table 31 for feeding water is installed at the other end of the observation box 2; an adjustment support assembly 32 for adjusting the height of the floating feeding table 31 is installed on the lower side of both ends of the observation box 2; an electronic scale 33 for weighing the floating feeding table 31 is provided on the top of the adjustment support assembly 32;

[0049] A collecting mechanism 4 is installed on the base 1 facing the lower side of the observation box 2 to collect and store the mouse feces in the plurality of observation boxes 2 according to the time and order of defecation.

[0050] In this embodiment, when the observation device based on toxicity test works normally, multiple groups of devices are set, and multiple mice are selected and divided into three groups a, b and c; during the experiment, the mice are administrated orally or intragastrically; the mice are placed in the observation box body 21, the observation box body 21 is closed and locked by the locking component 22, and then the mice are raised until the set time of the experiment ends;

[0051] In this process, the floating feeding tables 31 for feeding and watering are respectively arranged at the two ends of the observation box body 21, so as to prevent the mice from staying at the same end continuously; when the mice leave the floating feeding table 31 at one end, the electronic scale 33 at the lower side of the floating feeding table 31 is reset to zero, and when the mice return to the floating feeding table 31, the weight of the mice on the floating feeding table 31 is weighed by the electronic scale 33;

[0052] After the mice are medicated, the floating feeding platforms 31 at both ends are adjusted to a lower position by adjusting the support assembly 32, so that the mice can normally move to the floating feeding platform 31 to eat. In the subsequent feeding process, the height of the floating feeding platform 31 is gradually raised by adjusting the support assembly 32. If the mice cannot or find it difficult to move to the floating feeding platform 31 after the floating feeding platform 31 is raised, the height of the floating feeding platform 31 can be lowered; until the mice are able to climb normally to the floating feeding platform 31 at a higher position, it is determined that the mice have recovered their mobility.

[0053] The arrangement of the observation box body 21, the locking assembly 22 and the blocking assembly 23 facilitates the removal of mice from the observation box body 21, thereby preventing the mice from escaping from the observation box body 21 when the mice are removed.

[0054] The feces produced by the mice are collected and stored in real time by the collecting mechanism 4, and the time of each defecation of the mice can be recorded by the collecting mechanism 4, so that the experimenter does not need to collect the feces of the mice in real time and record the defecation time; the record is more accurate, the collection and storage are more timely, and the accuracy of the feces sample is guaranteed to the maximum extent; by detecting the components of the mouse feces sample, it is determined whether there are toxic drug residues and the residual amount in the mouse feces, as well as the change of the toxic drug residue in the mouse feces over time;

[0055] In the early stage of preparation, mice in group A were initially given a small dose, and after the mice were observed to recover, the dose was increased and given again, and this was repeated several times until the dose of mice in group A was close to the lethal dose range, thereby cultivating drug resistance in mice; mice in groups B and C were not given any drug in the initial state;

[0056] When the dosage of mice in group A is close to the lethal dosage range, the dosage is increased and administered to mice in groups A and B at the same time; when the mortality rates of mice in groups A and B are both low and lower than 20%, if the mortality rate of mice in group A is greater than that of mice in group B, then the administration of drugs to mice in group A during the early feeding stage caused short-term irreversible damage to the mice; if the mortality rate of mice in group A is not significantly different from that of mice in group B, then the administration of drugs to mice in group A during the early feeding stage did not cause short-term irreversible damage to the mice;

[0057] Then, all the surviving mice are put into group A, and mice from group C are selected and put into group B; after a period of feeding, the mice regain their mobility, eat normally, and their weight is not significantly reduced compared with the last weighing, and there is no toxic drug residue in the feces; the mice can be taken out to test other physical signs of the mice, and the dosage is increased again to give the drugs to the mice in groups A and B; this operation is repeated until the mortality rate of one group of mice in groups A and B is greater than 90%, and the experiment is terminated;

[0058] During this process, the mortality rates of mice in group A and group B were continuously observed; if the mortality rate of mice in group A was consistent with that of mice in group B, then during this test period, mice in group A did not show obvious drug resistance in the early feeding; if the mortality rate of mice in group A was significantly lower than that of mice in group B, such as the difference in mortality rates was more than twice, then the mice in group A developed drug resistance in the early feeding stage; if the mortality rate of mice in group A was significantly higher than that of mice in group B, such as the difference in mortality rates was more than twice, then the drug administration to mice in group A during the last feeding stage caused short-term and irreversible damage to the mice.

[0059] Embodiment 2: In some embodiments, Figure 1-Figure 9As shown, as a preferred embodiment of the present invention, the observation box 2 includes an observation box body 21, a locking assembly 22 and a blocking assembly 23, the observation box body 21 is mounted on the base 1, the locking assembly 22 is mounted on the observation box body 21, and the blocking assembly 23 is mounted on the observation box body 21;

[0060] The observation box body 21 includes a first support frame 211, a first support platform 212, a box body 213, a side cover 214, a feeding port 215, a middle cover 216 and a bottom structure. The two ends of the box body 213 are respectively fixedly mounted on the upper side of a first support platform 212, the first support platform 212 is fixedly mounted on the upper side of the first support frame 211, and the first support frame 211 is fixedly mounted on the upper side of the base 1; the middle cover 216 is rotatably mounted on the middle of the top of the box body 213, and the two side covers 214 are symmetrically arranged at the two ends of the box body 213, and one side of the side cover 214 The side cover 214 is rotatably connected to the top of the box body 213, and a feeding port 215 is provided on the side of the side cover 214; a bottom structure is installed at the bottom of the box body 213; a floating feeding table 31 is symmetrically installed at both ends of the bottom of the box body 213; the box body 213, the side cover 214, the middle cover 216, the bottom structure and the floating feeding table 31 constitute a moving space for mice; a locking component 22 for locking the side cover 214 is installed on the box body 213 and the side cover 214, and a locking component 22 for locking the middle cover 216 is installed between the box body 213 and the middle cover 216;

[0061] The bottom structure includes a collecting bucket 217 and round rods 218. A plurality of round rods 218 are fixedly installed at the bottom of the box body 213. A collecting bucket 217 with a larger top and a smaller bottom is fixedly installed at the bottom of the box body 213.

[0062] The locking assembly 22 includes a locking hole 221 and a locking pin 222. The box body 213, the side cover 214 and the middle cover 216 are provided with a locking hole 221 on one side away from the hinge point; the locking hole 221 on the box body 213 is aligned with the locking holes 221 on the side cover 214 and the middle cover 216; the locking pin 222 is plugged into the locking hole 221;

[0063] The blocking assembly 23 includes a mounting block 231, a plug rod 232 and a slot. The plug rods 232 are evenly and evenly fixedly installed on the mounting block 231. The side of the box body 213 is symmetrically provided with a through slot, and the slot is located between the side cover 214 and the middle cover 216. The plug rod 232 is plugged into the slot.

[0064] The floating feeding platform 31 includes a slide bar 311, a limit block 312, a support plate 313, a feeding trough 314 and a baffle 315. The support plate 313 is located in the box 213. A plurality of slide bars 311 are symmetrically fixedly installed on the lower side of the support plate 313. The bottom of the slide bar 311 passes through the lower side of the box 213 and is fixedly connected to the limit block 312. The slide bar 311 is in limited sliding connection with the box 213. A feeding trough 314 is fixedly installed on the upper side of the outer end of the support plate 313, and the feeding trough 314 is aligned with the feeding port 215. A baffle 315 is fixedly installed on the lower side of the inner end of the support plate 313.

[0065] The adjustable support assembly 32 includes a first motor 321, a screw rod 322, a movable sleeve rod 323, a guide rod 324 and a second support platform 325. The first motor 321 is fixedly mounted on the lower side of the first support frame 211. The output end of the first motor 321 passes through the first support frame 211 and the first support platform 212 and is fixedly connected to the lower end of the screw rod 322. The lower side of the movable sleeve rod 323 is sleeved outside the screw rod 322, and the screw rod 322 is threadedly connected to the movable sleeve rod 323 through a threaded sleeve; the second support platform 325 is fixedly mounted on the top of the movable sleeve rod 323, and the guide rod 324 is symmetrically fixedly mounted on the lower side of the second support platform 325, and the guide rod 324 is limitedly slidably connected to the first support platform 212; an electronic scale 33 useful for weighing the support plate 313 is fixedly mounted on the upper side of the second support platform 325;

[0066] The collecting mechanism 4 includes a film 41, a feeding roller 42, a guide roller 43, an adjusting roller 44, a feeding roller 45, a winding roller 46 and a second support frame 47. The second support frame 47 is symmetrically fixedly installed on the base 1, and the feeding roller 42 for driving the film 41 to unwind, the guide roller 43 for guiding and supporting the film 41, the adjusting roller 44 for adjusting the tension of the film 41, the feeding roller 45 for driving the film 41 to move to the right at a uniform speed and the winding roller 46 for winding the film 41 are rotatably installed on the second support frame 47 from left to right. The film 41 between the feeding roller 45 and the winding roller 46 is aligned with the lower side of multiple collecting buckets 217.

[0067] The unwinding roller 42, the feeding roller 45 and the winding roller 46 are all driven by a motor, and a servo motor or a reduction motor can be used. The feeding roller 45 includes an active roller and a driven roller, which are rotatably mounted on the second support frame 47, and the active roller and the driven roller clamp the film 41 at the upper and lower sides of the film 41. The motor drives the active roller to rotate, and the active roller conveys the film 41 at a uniform speed through the cooperation of the driven roller.

[0068] In this embodiment, when the observation box 2, the detection mechanism 3 and the collection mechanism 4 are working normally, the locking pin 222 locks the side cover 214 and the middle cover 216 with the box body 213 through the locking hole 221; before taking the mouse out of the box body 213, the position of the mouse can be observed, and the insertion rod 232 can be inserted into the slot to separate the box body 213 through the insertion rod 232, so as to prevent the mouse from escaping to other areas in the box body 213 when the mouse is taken out; then the locking pin 222 corresponding to the area where the mouse is located is pulled out, so that the locking pin 222 no longer blocks the movement of the upper side side cover 214 or the middle cover 216, and the side cover 214 or the middle cover 216 is flipped open, and the mouse is taken out or put in, and then the insertion rod 232 is pulled out so that it does not affect the movement of the mouse in the box body 213;

[0069] The first motor 321 drives the screw rod 322 to rotate, and the rotation of the screw rod 322 drives the movable sleeve rod 323 and the second support platform 325 to move. The movable sleeve rod 323 and the second support platform 325 move vertically under the limiting action of the guide rod 324, thereby pushing the support plate 313 to move through the electronic scale 33. The support plate 313 moves vertically in the box body 213 under the limiting action of the sliding rod 311 and the limiting block 312, thereby adjusting the position of the support plate 313, and preventing mice from escaping from the lower side of the support plate 313 through the baffle plate 315;

[0070] Feed food or water into the feeding trough 314 through the feeding port 215, and set the feeding troughs 314 for feeding food and water at both ends of the box 213 respectively, so as to prevent the mouse from always staying in one position; after the mouse leaves the support plate 313, adjust the electronic scale 33 to zero, and after the mouse returns to the support plate 313, use the electronic scale 33 to detect the weight of the mouse, so that the mouse does not need to be taken out of the box 213, and the weight of the mouse can be measured before the mouse eats;

[0071] During the test, the winding roller 46 winds up the film 41 at a relatively low speed through the cooperation of the unwinding roller 42, the guide roller 43, the adjusting roller 44 and the feeding roller 45. During this process, the linear speed of the film 41 is kept constant. The feces produced by the test mice fall through the criss-crossed round rods 218 and fall to the upper side of the film 41 between the feeding roller 45 and the winding roller 46 under the guidance of the collecting bucket 217. The winding roller 46 winds up the film 41 and the mouse feces on the film 41 at the same time. The mouse feces are collected in time by the film 41, and the rolled-up film 41 wraps the feces to preserve the film 41. By rolling up the film 41 at a uniform speed, the defecation time corresponding to the feces can be calculated according to the location of the feces, so that when analyzing the feces components, the toxic drug residues corresponding to the feces can be obtained, as well as the changes of the toxic drug residues in the feces over time.

[0072] Embodiment 3: In some embodiments, Figure 1-Figure 9 As shown, as a preferred embodiment of the present invention, a test method of an observation device based on toxicity testing comprises the following steps:

[0073] Step 1: Select a number of mice and divide them into three groups: a, b and c; during the experiment, the mice are administrated orally or intragastrically;

[0074] Step 2: administering drugs to mice in group A, and increasing the dosage after observing the recovery of the mice, and administering drugs again, and repeating the process multiple times until the dosage of mice in group A is close to the lethal dosage range, so as to cultivate drug resistance in mice; mice in groups B and C are not administered drugs in the initial state; placing the mice in the observation box body 21, closing the observation box body 21 and locking it through the locking component 22, and monitoring the physical signs of the mice in the observation box body 21;

[0075] Step 3: When the mouse leaves the floating feeding table 31 at one end, the electronic scale 33 at the lower side of the floating feeding table 31 is reset to zero, and when the mouse returns to the floating feeding table 31, the weight of the mouse on the floating feeding table 31 is weighed by the electronic scale 33;

[0076] Step 4: After the mice are medicated, the floating feeding platforms 31 at both ends are adjusted to a lower position by adjusting the support assembly 32, so that the mice can normally move to the floating feeding platform 31 to eat. In the subsequent feeding process, the height of the floating feeding platform 31 is gradually raised by adjusting the support assembly 32. If the mice cannot or find it difficult to move to the floating feeding platform 31 after the floating feeding platform 31 is raised, the height of the floating feeding platform 31 can be lowered; until the mice are able to climb normally to the floating feeding platform 31 at a higher position, it is determined that the mice have recovered their mobility;

[0077] Step 5: While performing steps 1, 2, and 3, the mouse feces are collected and stored in real time by the collecting mechanism 4, and the time of each defecation of the mouse can be recorded by the collecting mechanism 4, so that the experimenter does not need to collect the mouse feces in real time and record the defecation time; the record is more accurate, the collection and storage are more timely, and the accuracy of the feces sample is guaranteed to the maximum extent; by detecting the components of the mouse feces sample, it is determined whether there are toxic drug residues and the residual amount in the mouse feces, as well as the change of the toxic drug residue in the mouse feces over time;

[0078] Step 6: Repeat steps 2, 3, 4 and 5 until the dosage of mice in group A is close to the lethal dosage range, and increase the dosage to mice in groups A and B at the same time; observe the status and mortality of mice in groups A and B, and draw corresponding experimental conclusions;

[0079] Step 7: All surviving mice are assigned to group a, and mice from group c are selected to group b; after a period of feeding, when the mice recover, the dosage is increased again and administered to the mice in groups a and b; the status and mortality of the mice in groups a and b are observed, and corresponding experimental conclusions are drawn; and this operation is repeated until the mortality of one group of mice in groups a and b is greater than 90%, and the experiment is terminated.

[0080] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An observation device based on toxicity testing, comprising a base station (1), characterized in that: A plurality of long strip-shaped observation boxes (2) are installed on the base platform (1); a detection mechanism (3) for detecting the weight and exercise ability of mice is installed on the observation box (2); The detection mechanism (3) comprises a floating feeding platform (31), an adjustment support assembly (32) and an electronic scale (33); a floating feeding platform (31) for feeding is installed at one end of the observation box (2), and a floating feeding platform (31) for feeding water is installed at the other end of the observation box (2); an adjustment support assembly (32) for adjusting the height of the floating feeding platform (31) is installed at the lower side of both ends of the observation box (2); an electronic scale (33) for weighing the floating feeding platform (31) is arranged on the top of the adjustment support assembly (32); A collecting mechanism (4) is installed on the base (1) so as to be aligned with the lower side of the observation box (2) and is used to collect and store the mouse feces in the plurality of observation boxes (2) according to the time and sequence of defecation.

2. The observation device based on toxicity test according to claim 1, characterized in that: The observation box (2) comprises an observation box body (21), a locking assembly (22) and a blocking assembly (23); the observation box body (21) is mounted on a base platform (1); the locking assembly (22) is mounted on the observation box body (21); and the blocking assembly (23) is mounted on the observation box body (21).

3. The observation device based on toxicity test according to claim 2, characterized in that: The observation box body (21) comprises a first support frame (211), a first support platform (212), a box body (213), side covers (214), a feeding port (215), an intermediate cover (216) and a bottom structure. The two ends of the box body (213) are respectively fixedly mounted on the upper side of a first support platform (212), the first support platform (212) is fixedly mounted on the upper side of the first support frame (211), and the first support frame (211) is fixedly mounted on the upper side of the base (1); the intermediate cover (216) is rotatably mounted in the middle of the top of the box body (213), the two side covers (214) are symmetrically arranged at the two ends of the box body (213), and one side of the side cover (214) is fixedly mounted on the upper side of the base (1). The side cover (214) is rotatably connected to the top of the box body (213), and a feeding port (215) is provided on the side of the side cover; a bottom structure is installed at the bottom of the box body (213); a floating feeding platform (31) is symmetrically installed at two ends of the bottom of the box body (213); a moving space for mice is formed between the box body (213), the side cover (214), the middle cover (216), the bottom structure and the floating feeding platform (31); a locking assembly (22) for locking the side cover (214) is installed on the box body (213) and the side cover (214), and a locking assembly (22) for locking the middle cover (216) is installed between the box body (213) and the middle cover (216).

4. The observation device based on toxicity test according to claim 3, characterized in that: The bottom structure comprises a collecting bucket (217) and round rods (218); a plurality of crisscrossing round rods (218) are fixedly installed on the bottom of the box body (213); and a collecting bucket (217) which is larger at the top and smaller at the bottom is fixedly installed on the bottom of the box body (213).

5. The observation device based on toxicity test according to claim 4, characterized in that: The locking assembly (22) comprises a locking hole (221) and a locking pin (222); the locking hole (221) is provided on a side of the box body (213), the side cover (214) and the middle cover (216) away from the hinge point; the locking hole (221) on the box body (213) is aligned with the locking holes (221) on the side cover (214) and the middle cover (216); and the locking pin (222) is plugged into the locking hole (221).

6. The observation device based on toxicity test according to claim 5, characterized in that: The blocking assembly (23) comprises a mounting block (231), an insertion rod (232) and a slot; the insertion rods (232) are evenly and evenly fixedly mounted on the mounting block (231); the side edges of the box body (213) are symmetrically provided with through slots, and the slots are located between the side cover (214) and the middle cover (216); and the insertion rods (232) are plugged into the slots.

7. The observation device based on toxicity test according to claim 6, characterized in that: The floating feeding platform (31) comprises a sliding rod (311), a limiting block (312), a supporting plate (313), a feeding trough (314) and a baffle (315); the supporting plate (313) is located in a box body (213); a plurality of sliding rods (311) are symmetrically fixedly installed on the lower side of the supporting plate (313); the bottom of the sliding rod (311) passes through the lower side of the box body (213) and is fixedly connected to the limiting block (312); the sliding rod (311) is limitedly slidably connected to the box body (213); a feeding trough (314) is fixedly installed on the upper side of the outer end of the supporting plate (313), and the feeding trough (314) is aligned with the feeding port (215); and a baffle (315) is fixedly installed on the lower side of the inner end of the supporting plate (313).

8. The observation device based on toxicity test according to claim 7, characterized in that: The adjusting support assembly (32) comprises a first motor (321), a screw rod (322), a movable sleeve rod (323), a guide rod (324) and a second support platform (325); the first motor (321) is fixedly mounted on the lower side of the first support frame (211); the output end of the first motor (321) passes through the first support frame (211) and the first support platform (212) and is fixedly connected to the lower end of the screw rod (322); the lower side of the movable sleeve rod (323) is sleeved on the Outside the screw rod (322), the screw rod (322) is threadedly connected to the movable sleeve rod (323) through a threaded sleeve; a second support platform (325) is fixedly installed on the top of the movable sleeve rod (323); a guide rod (324) is symmetrically fixedly installed on the lower side of the second support platform (325); the guide rod (324) is limitedly slidably connected to the first support platform (212); an electronic scale (33) used to weigh the support plate (313) is fixedly installed on the upper side of the second support platform (325).

9. The observation device based on toxicity test according to claim 8, characterized in that: The collecting mechanism (4) comprises a film (41), a feeding roller (42), a guide roller (43), an adjusting roller (44), a feeding roller (45), a winding roller (46) and a second support frame (47); the second support frame (47) is symmetrically fixedly mounted on the base (1); the feeding roller (42) for driving the film (41) to unwind, the guide roller (43) for guiding and supporting the film (41), the adjusting roller (44) for adjusting the tension of the film (41), the feeding roller (45) for driving the film (41) to move rightward at a uniform speed and the winding roller (46) for winding the film (41) are rotatably mounted on the second support frame (47) from left to right in sequence; the film (41) between the feeding roller (45) and the winding roller (46) is aligned with the lower side of the plurality of collecting buckets (217).

10. A test method for an observation device based on toxicity testing, using the observation device based on toxicity testing according to claim 9, characterized in that: The following steps are involved: Step 1: Select a number of mice and divide them into three groups: a, b and c; Mice were administrated orally or by gavage during the experiments; Step 2: administering the drug to mice in group A, and increasing the dosage after observing the recovery of the mice, and administering the drug again, and repeating the process multiple times until the dosage of mice in group A is close to the lethal dose range, thereby cultivating drug resistance in the mice; mice in groups B and C are not administered the drug in the initial state; placing the mice in an observation box body (21), closing the observation box body (21) and locking it through a locking component (22), and monitoring the vital signs of the mice in the observation box body (21); Step 3: When the mouse leaves the floating feeding platform (31) at one end, the electronic scale (33) on the lower side of the floating feeding platform (31) is reset to zero, and when the mouse returns to the floating feeding platform (31), the weight of the mouse on the floating feeding platform (31) is weighed by the electronic scale (33); Step 4: After the mice are administrated, the floating feeding platforms (31) at both ends are adjusted to a lower position by adjusting the support assembly (32), so that the mice can normally move to the floating feeding platform (31) to eat. In the subsequent feeding process, the height of the floating feeding platform (31) is gradually raised by adjusting the support assembly (32). If the mice cannot or have difficulty moving to the floating feeding platform (31) after raising the height of the floating feeding platform (31), the height of the floating feeding platform (31) can be lowered. The mice are allowed to climb to the floating feeding platform (31) at a higher position normally, and the mice are judged to have recovered their mobility. Step 5: While carrying out steps 1, 2 and 3, the mouse feces are collected and stored in real time by the collecting mechanism (4), and the time of each defecation of the mouse can be recorded by the collecting mechanism (4), so that the experimenter does not need to collect the mouse feces in real time and record the defecation time; the record is more accurate, the collection and storage are more timely, and the accuracy of the feces sample is guaranteed to the maximum extent; by detecting the components of the mouse feces sample, it is determined whether there are toxic drug residues and the residual amount in the mouse feces, as well as the change of the toxic drug residue in the mouse feces over time; Step 6: Repeat steps 2, 3, 4 and 5 until the dosage of mice in group A is close to the lethal dosage range, and increase the dosage to mice in groups A and B at the same time; observe the status and mortality of mice in groups A and B, and draw corresponding experimental conclusions; Step 7: All surviving mice are assigned to group a, and mice from group c are selected to group b; after a period of feeding, when the mice recover, the dosage is increased again and administered to the mice in groups a and b; the status and mortality of the mice in groups a and b are observed, and corresponding experimental conclusions are drawn; and this operation is repeated until the mortality of one group of mice in groups a and b is greater than 90%, and the experiment is terminated.

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