Application of terpenoid in preparation of preparation for improving salt resistance and / or drought resistance of plants

Abstract

The invention discloses application of terpenoids in preparation of a preparation for improving salt resistance and / or drought resistance of plants. The terpenoid is abietic acid (AA), is applied to seeds, seedlings or grown-up seedlings of plants such as wheat, millet, corn and the like subjected to salt stress and / or drought stress through a soaking or irrigation method, and can remarkably improve the germination rate, bud length, root length and fresh weight of the plant seeds under the salt stress and the drought stress; the survival rate, plant height, root length, fresh weight, chlorophyll content, single spike weight and single spike grain number of plant seedlings are improved, and the salt stress and drought stress resistance of plants is improved. The application of AA under normal conditions has no adverse effect on the growth and development of plants. Therefore, AA plays an important role in improving the salt tolerance and drought resistance of plants. The application method provided by the invention is simple in use method, remarkable in effect, safe, reliable and huge in application potential, and meets the requirements of agricultural sustainable development of saline-alkali soil and dry land.

Description

Technical Field

[0001] The invention belongs to the technical field of plant planting, and particularly relates to the application of a terpene compound in preparing a preparation for improving the salt tolerance and / or drought resistance of plants. Background Art

[0002] During their growth, plants are often subjected to a variety of stresses, which in turn affect their growth and development, and reduce their yield and quality. Salt stress and drought stress are two major abiotic stresses that plants often face, causing physiological drought, ion toxicity and oxidative stress to plants, inhibiting plant growth, and in severe cases leading to plant death, ultimately reducing crop yields. Therefore, how to improve the salt and drought tolerance of crops has become a global concern.

[0003] Terpenoids, as a class of plant secondary metabolites, have a wide range of pharmacological effects, aromatic qualities and biological activities, and have shown wide application potential in many fields such as medicine, health products, cosmetics, pesticides, etc. Abietic acid (AA) is a tricyclic diterpenoid oxygen-containing compound, slightly soluble in water, and easily soluble in ether, ethanol, benzene, acetone, chloroform, and carbon disulfide. In medicine, abietic acid (AA) has significant therapeutic effects on cardiovascular and cerebrovascular diseases, hypertension, and diseases caused by acidic body fluids. It has multiple pharmacological effects such as anti-tumor, anti-inflammatory, and antibacterial. It has obvious health effects in anti-oxidation, enhancing immunity, maintaining alkaline physique, and improving the comprehensive ability of the body. AA has a natural, safe, unique fragrance and biological activity. Therefore, abietic acid (AA) has shown wide application potential in many fields such as medicine, health products, cosmetics, and pesticides. However, there is no report on the direct effect of AA on plant salt tolerance and drought resistance. Summary of the invention

[0004] In view of the above-mentioned prior art, the purpose of the present invention is to provide an application of a terpenoid compound in the preparation of a preparation for improving plant salt tolerance and / or drought resistance. The application technical solution provided by the present invention plays an important role in improving plant salt tolerance and drought resistance.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: The invention provides an application of a terpenoid compound in preparing a preparation for improving the salt tolerance and / or drought resistance of plants. The terpenoid compound is abietic acid.

[0006] Furthermore, the application method is: preparing an aqueous solution containing rosin acid and applying it to seeds, seedlings or mature seedlings of plants.

[0007] Furthermore, the specific steps of the application are: first wash the seeds twice with 10% Tween water (Tween-20), then disinfect the seed surface with 70% anhydrous ethanol for 5 seconds, and then wash with sterile water for 5-6 times, and soak the plant seeds in the rosin acid aqueous solution; or soak the roots of plant seedlings or mature seedlings in the rosin acid aqueous solution.

[0008] Furthermore, the concentration of rosin acid in the rosin acid aqueous solution is 0.1 μmol / L to 60 μmol / L.

[0009] Furthermore, during the seed germination period, the concentration of abietic acid used was 10 μM ~50 μM.

[0010] Furthermore, during the seedling stage, the concentration of abietic acid used was 1 μM~15 μM.

[0011] Furthermore, during the seedling stage, the concentration of abietic acid used was 5 μM~15 μM.

[0012] Furthermore, the plant seeds are treated with the rosin acid solution at a rate of 0.1-0.5 mL / seed at 25-30° C. for 3-5 days.

[0013] Furthermore, the three-leaf seedlings or jointing-stage mature seedlings of the plants are immersed in 50-100 mL / plant; immersed once every 5 to 7 days, and immersed 2 to 4 times in succession.

[0014] Furthermore, the plants include seeds, seedlings or seedlings of millet, wheat and corn.

[0015] Furthermore, the rosin acid can increase the seed germination rate of plants, increase the survival rate of seedlings, increase the bud length of seedlings, increase the root length of seedlings, and increase the fresh weight of seedlings under salt stress.

[0016] Furthermore, the rosin acid can improve the survival rate of plants under drought stress, increase the bud length of seedlings, increase the root length of seedlings, increase the chlorophyll content of seedlings, and increase the fresh weight of seedlings.

[0017] Furthermore, the rosin acid can increase the single ear weight and single ear grain number of plants under salt stress.

[0018] Compared with the prior art, the advantages and positive effects of the present invention are as follows: the present invention provides the application of rosin acid in improving the salt tolerance and drought resistance of crops. As a terpenoid compound, rosin acid can improve the plant seed germination rate, seedling survival rate, seedling root length, seedling bud length, and seedling fresh weight under salt stress and drought stress conditions, improve the plant seedling survival rate, seedling plant height, seedling root length, seedling chlorophyll content, single ear weight, and single ear grain number, reduce salt damage and drought damage, increase yield, and achieve the purpose of improving salt stress and drought stress resistance in production. The use method of the present invention is simple and effective, safe and reliable, harmless to plant and human health, conducive to improving planting income, and has a wide range of application prospects in agricultural production. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is the experimental result of promoting millet seed germination under salt stress by exogenous application of AA; Figure 2-1 Results of the experiment on promoting wheat seed germination under salt stress by exogenous AA Figure 1 ; Figure 2-2 Figure 2 shows the experimental results of promoting wheat seed germination under salt stress by exogenous application of AA; Figure 3 This is the experimental result of improving the salt tolerance of millet seedlings by exogenous application of AA; Figure 4 This is the experimental result of improving the salt tolerance of wheat seedlings by exogenous application of AA; Figure 5 This is the experimental result of improving the salt tolerance of millet seedlings by exogenous application of AA; Figure 6 This is the experimental result of improving the salt tolerance of corn seedlings by applying AA exogenously; Figure 7 This is the result of the experiment on improving the drought resistance of millet seedlings by applying AA exogenously; Figure 8 This is the result of the experiment on improving drought resistance of wheat seedlings by exogenous application of AA; Fig. 9 This is the result of the experiment on improving drought resistance of corn seedlings by applying AA exogenously; Fig.10 This is the experimental result of exogenous application of AA to increase the single ear weight and single ear grain number of millet under salt stress. DETAILED DESCRIPTION

[0020] The technical solution of the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods. However, the present invention is not limited to the following implementation methods, and the methods described are conventional methods unless otherwise specified. The raw materials described can be obtained from public commercial channels unless otherwise specified.

[0021] Example 1: Exogenous application of AA promotes millet seed germination under salt stress 1. Dissolve 3.0245 mg of abietic acid (AA) in 1 mL of anhydrous ethanol to prepare a 10 mmol / L stock solution, and then dilute it with water to a solution containing 0.1 μmol / L-60 μmol / L AA as the subsequent salt stress or drought stress treatment solution.

[0022] 2. Select plump and healthy millet seeds, first wash the seeds twice with 10% Tween water (Tween-20), then disinfect the seed surface with 70% anhydrous ethanol for 5 seconds, and then wash with sterile water 5-6 times. Set up three groups of experiments, namely the control group (divided into normal control group and salt treatment control group) and the experimental group (salt treatment experimental group), each group has three parallel experiments, and 35 millet seeds are selected for each parallel. Spread the soaked seeds on a 9 cm glass culture dish with sterilized filter paper placed in advance, and culture at 25°C; the normal control group is treated with 5 mL of aqueous solution, the salt treatment control group is treated with 5 mL of 150mM NaCl, and the salt treatment experimental group is treated with 150 mM NaCl containing 1 μM AA, 5 μM AA, 10 μM AA, and 15 μM AA. Culture for 1 to 5 days and count the seed germination data.

[0023] Figure 1 CK is distilled water, S 150 is 150 mM NaCl. Figure 1 It can be seen that compared with the salt treatment control group, exogenous application of AA can significantly increase the germination rate, cotyledon greening rate, sprout length, root length and fresh weight of millet seeds, significantly promote the germination of millet seeds, and improve the salt tolerance of millet during the germination period.

[0024] Example 2: Exogenous application of AA promotes wheat seed germination under salt stress 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0025] 2. Select full and healthy wheat seeds, select 20 seeds for each parallel, and treat with 175 mM NaCl for salt stress. Seed treatment method, experimental setting and data statistics are the same as in Example 1. Cultivate for 1 to 4 days and count the seed germination data.

[0026] Depend on Figure 2-1 It can be seen that compared with the salt treatment control group, exogenous application of AA can significantly increase the germination rate, cotyledon greening rate, sprout length, root length and fresh weight of wheat seeds, significantly promote the germination of wheat seeds, and improve the salt tolerance of wheat during the germination period.

[0027] The seeds were treated with 175 mM NaCl containing 10 μM AA, 25 μM AA, 50 μM AA, 75 μM AA, and 100 μM AA for 4 days, and the seed germination data were counted.

[0028] Depend on Figure 2-2 It can be seen that within the concentration range of 10 μM ~50 μM, AA increased the germination rate, root length, stem length and fresh weight of wheat; within the range of 75 μM ~100 μM, AA reduced the germination rate, root length and shoot length of wheat. Therefore, the appropriate concentration of AA for germination is 25 μM.

[0029] Example 3: Exogenous application of AA improves salt tolerance of millet seedlings 1. The preparation method of AA treatment solution, seed dosage, seed treatment, salt stress concentration and experimental settings are the same as those in Example 1.

[0030] 2. The salt-treated control group was treated with 5 mL of 150 mM NaCl, and the salt-treated experimental group was treated with 150 mM NaCl solution containing 0.1 μM AA, 0.5 μM AA, 1 μM AA, and 10 μM AA for 7 days, and the relevant data were collected.

[0031] Depend on Figure 3 It can be seen that compared with the salt treatment control group, exogenous application of AA under salt treatment can increase the plant height and root length of millet seedlings and improve the salt tolerance of millet seedlings.

[0032] Example 4: Exogenous application of AA improves salt tolerance of wheat seedlings 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0033] 2. Select plump and healthy millet seeds, first wash the seeds twice with 10% Tween water (Tween-20), then disinfect the seed surface with 70% anhydrous ethanol for 20 seconds, and then wash with sterile water 5-6 times. Set up three groups of experiments, namely the control group (divided into normal control group and salt treatment control group) and the experimental group (salt treatment experimental group), and set up three parallel experiments in each group. After 3 days of wheat germination, select seedlings with consistent growth, spread them on an 18 cm glass culture dish with sterilized filter paper placed in advance, and culture them at 25°C; the normal control group is treated with 5 mL of aqueous solution, the salt treatment control group is treated with 5 mL of 175 mM NaCl, and the salt treatment experimental group is treated with 175 mM NaCl containing 1 μM AA, 5 μM AA, 10 μM AA, and 15 μM AA. After 7 days of culture, the relevant data are counted.

[0034] Depend on Figure 4It can be seen that compared with the salt stress control group, exogenous application of AA under salt stress can increase the plant height, root length and fresh weight of wheat seedlings and improve the salt tolerance of wheat seedlings. In the concentration range of 1-10 μM AA, the plant height, root length and fresh weight of wheat seedlings increased in a dose-dependent manner. The effect of 15 μM AA began to weaken.

[0035] Example 5: Exogenous application of AA improves salt tolerance of millet seedlings 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0036] 2. Select plump and healthy millet seeds. Plant 20 millet seeds in each black plastic pot filled with nutrient soil. After the millet germinates for 3 days, select 15 seedlings with consistent growth for retention. These 15 seedlings will continue to grow for 3 days before being treated. Set up three groups of experiments, namely the control group (divided into a normal control group and a salt-treated control group) and the experimental group (salt-treated experimental group). Each group has three parallel experiments (each 6 pots as a group). The normal control group was soaked in clean water, the salt-treated control group was soaked in 2 L of 300 mM NaCl solution, and the salt-treated experimental group was soaked in 2 L of 300 mM NaCl solution containing 5 μM AA, 10 μM AA, and 15 μM AA. Ensure that the soaking volume of each plant is 50-100 mL, soak for 12 hours, soak once every 7 days, and soak twice in total. Continue to grow for 7 days after the last soaking, and count the relevant data.

[0037] Depend on Figure 5 It can be seen that compared with the salt stress control group, exogenous application of AA under salt stress conditions can significantly increase the survival rate, chlorophyll content, plant height, root length and fresh weight of millet seedlings, and significantly improve the salt tolerance of millet. The survival rate, fresh weight and chlorophyll content of millet treated with 10 μM AA all showed the highest values.

[0038] Example 6: Exogenous application of AA improves salt tolerance of corn seedlings 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0039] 2. Select plump and healthy corn seeds. Plant 6 corn seeds in each black plastic pot filled with nutrient soil. After the corn germinates for 2 days, select 5 seedlings with consistent growth for retention. These 5 seedlings will continue to grow for 3 days before treatment. Set up three groups of experiments, namely the control group (divided into a normal control group and a salt-treated control group) and the experimental group (salt-treated experimental group). Each group has three parallel experiments (each 6 pots as a group). The normal control group was soaked in clean water, the salt-treated control group was soaked in 2 L of 300 mM NaCl solution, and the salt-treated experimental group was soaked in 2 L of 300 mM NaCl solution containing 5 μM AA, 10 μM AA, and 20 μM AA. Ensure that the soaking volume of each plant is 50-100 mL, soak for 12 hours, soak once every 7 days, and soak twice in total. Continue to grow for 7 days after the last soaking, and count the relevant data.

[0040] Depend on Figure 6 It can be seen that compared with the salt stress control group, exogenous application of AA solution under salt stress conditions can significantly increase the survival rate, plant height, fresh weight and chlorophyll content of corn, and significantly improve the salt tolerance of corn seedlings. The survival rate, fresh weight and chlorophyll content of corn treated with 5 μM AA were the highest.

[0041] Example 7: Exogenous application of AA improves drought resistance of millet seedlings 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0042] 2. Select plump and healthy millet seeds. Plant 20 millet seeds in each black plastic pot filled with nutrient soil. After the millet germinates for 3 days, select 15 seedlings with consistent growth for retention. These 15 seedlings continue to grow for 3 days before being treated. Set up three groups of experiments, namely the control group (divided into a normal control group and a drought treatment control group) and the experimental group (drought treatment experimental group), and set up three parallel experiments in each group (each 6 pots as a group). The normal control group was soaked in clean water, the drought treatment control group was soaked in 3 L of clean water each time, and the drought treatment experimental group was soaked in 3 L of a solution containing 5 μM AA, 10 μM AA, and 15 μM AA each time, ensuring that the soaking volume of each plant was 50-100 mL, soaking for 12 hours, soaking once every 7 days, stopping watering after soaking twice, and starting drought treatment. After 20 days, the relevant data were counted.

[0043] Depend on Figure 7 It can be seen that compared with the drought stress control group, exogenous application of AA solution under drought stress conditions can significantly increase the survival rate, plant height, root length and fresh weight of millet, and significantly improve the drought resistance of millet. The effects of 5 μM ~15 μM AA are similar.

[0044] Example 8: Exogenous application of AA improves drought resistance of wheat 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0045] 2. Select full and healthy wheat seeds. Plant 12 wheat seeds in each black plastic pot filled with nutrient soil. After the wheat germinates for 2 days, select 6 seedlings with consistent growth for retention. These 6 seedlings continue to grow for 3 days before treatment. Set up three groups of experiments, namely the control group (divided into a normal control group and a drought treatment group) and the experimental group (drought treatment experimental group), and set up three parallel experiments in each group (each 6 pots as a group). The normal control group was soaked in clean water, the drought treatment control group was soaked in 3L of quantitative clean water each time, and watering was stopped after soaking twice. The drought treatment experimental group was soaked in 3L of a solution containing 5 μM AA, 10 μMAA, and 20 μM AA, ensuring that the soaking volume of each plant was 50-100 mL; soak for 12 hours, soak once every 7 days, stop watering after soaking twice, start drought treatment, and count relevant data after 25 days.

[0046] Depend on Figure 8 It can be seen that compared with the control group treated with drought stress, exogenous immersion in AA solution under drought stress conditions can significantly increase the survival rate, plant height, root length and fresh weight of wheat, and significantly improve the drought resistance of wheat, and 5 μM AA has a better effect.

[0047] Example 9: Exogenous application of AA improves drought resistance of corn 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0048] 2. Select plump and healthy corn seeds. Plant 6 corn seeds in each black plastic pot filled with nutrient soil. After the corn germinates for 2 days, select 5 seedlings with consistent growth for retention. These 5 seedlings continue to grow for 3 days before treatment. Set up three groups of experiments, namely the control group (divided into a normal control group and a drought treatment control group) and the experimental group (drought treatment experimental group). Each group has three parallel experiments (each 6 pots as a group). The normal control group was soaked in clean water, the drought treatment control group was soaked in 3L of clean water each time, and watering was stopped after soaking twice; the drought treatment experimental group was soaked in 3L of solution containing 5 μM AA, 10 μMAA, and 20 μM AA, ensuring that the soaking volume of each plant was 50-100 mL; soak for 12 hours, soak once every 7 days, stop watering after soaking twice, and start to see that compared with the drought treatment control group, exogenous soaking AA solution under drought stress conditions can significantly increase the survival rate, chlorophyll content and fresh weight of corn, and significantly improve the drought resistance of corn seedlings, and 5 μM AA has a better effect. Low concentration of AA had no significant effect on maize plant height, but high concentration of AA reduced maize plant height.

[0049] Example 10: Exogenous application of AA improves the weight and number of grains per ear of millet under salt tolerance 1. The preparation method of AA treatment solution is the same as that of Example 1.

[0050] 2. Select plump and healthy millet seeds. Plant 15 millet seeds in each black plastic pot filled with nutrient soil. After the millet germinates for 3 days, select 9 seedlings with consistent growth and remove the rest of the seedlings with tweezers. These 9 seedlings continue to grow for 3 days before processing. Set up three groups of experiments, namely the control group (divided into a normal control group and a salt-treated control group) and the experimental group (salt-treated experimental group). Each group has three parallel experiments (each 6 pots as a group). The normal control group was soaked in clean water, the salt-treated control group was soaked in 200 mM NaCl solution, and the salt-treated experimental group was soaked in 200 mM NaCl solution containing 10 μM AA; each soaking solution was quantitatively 2 L, ensuring that the soaking volume of each plant was 50-100 mL, and soaked 3 times (the first time was treated at the 3-leaf stage, the second time was treated at the jointing stage, and the third time was treated before heading), and then normal watering management was performed until the seeds were harvested, and relevant data were counted.

[0051] Depend on Fig.10 It can be seen that compared with the salt treatment control group, exogenous soaking in AA solution can significantly increase the weight and number of grains per ear of millet, and improve the yield of millet under salt stress.

[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions claimed to be protected by the present invention.

Claims

1. Use of a terpenoid compound in the preparation of a preparation for improving plant salt tolerance and / or drought resistance, characterized in that: The terpene compound is abietic acid.

2. The use according to claim 1, characterized in that: The application method comprises the following steps: preparing an aqueous solution containing rosin acid and applying the solution to seeds, seedlings or mature seedlings of plants.

3. The use according to claim 2, characterized in that: The specific steps of the application are: sterilizing the surface of plant seeds with ethanol, then washing with sterile water, soaking the plant seeds with the rosin acid aqueous solution; or soaking the roots of plant seedlings or mature seedlings with the rosin acid aqueous solution.

4. The use according to claim 2, characterized in that: The concentration of rosin acid in the rosin acid aqueous solution is 0.1 μmol / L to 60 μmol / L.

5. The application according to claim 4, characterized in that: During the seed germination stage, the concentration of rosin acid used is 10 μM ~50 μM; during the seedling stage, the concentration of rosin acid used is 1 μM ~15 μM; during the seedling stage, the concentration of rosin acid used is 5 μM ~15 μM.

6. The application according to claim 3, characterized in that: The usage amount of the rosin acid solution is 0.1-0.5 mL / seed, and the plant seeds are cultured at 25-30° C. for 3-5 days.

7. The use according to claim 3, characterized in that: The three-leaf seedlings or mature seedlings of the plants are soaked in the rosin acid aqueous solution at a dosage of 50-100 mL / plant, and the solution is soaked once every 5-7 days for 10-12 hours each time, and the soaking is continued for 2-4 times.

8. The use according to claim 3, characterized in that: The plants include millet, wheat, corn, soybean, and tomato.

9. The use according to claim 1, characterized in that: The rosin acid can increase seed germination rate, seedling sprout length, seedling root length, seedling fresh weight, seedling survival rate, seedling plant height, seedling fresh weight, seedling root length, seedling chlorophyll content, and increase single ear weight and single ear grain number under salt stress.

10. The use according to claim 1, characterized in that: The rosin acid can improve plant survival rate, increase plant height, increase root length, increase fresh weight and increase chlorophyll content under drought stress.

Images