Pesticide composition for preventing and treating strawberry powdery mildew

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating strawberry powdery mildew, the active component of the pesticide composition is prepared by compounding JS399-19 and bixpyrazinam or eugenol according to the mass ratio of (1-200): (200-1). JS399-19 and bixpyrazinam or eugenol are compounded, the compounded combination has an obvious synergistic interaction effect on alchemilla alchemilla which causes strawberry powdery mildew within a certain mass ratio range, and compared with any bactericidal active ingredient in the compounded combination, the control effect on strawberry powdery mildew can be improved, and the control effect on strawberry powdery mildew can be improved. Meanwhile, the drug resistance risk of using a single bactericidal active ingredient can be reduced, generation and accumulation of drug resistance of pathogenic bacteria can be delayed, pesticide residues and prevention and treatment cost can be reduced, support can be provided for development and development of a novel agent for preventing and treating strawberry powdery mildew, and the bactericidal composition has very important significance on green and healthy development of the strawberry industry.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling strawberry powdery mildew. Background Art

[0002] Strawberry is a berry fruit belonging to the genus Fragaria in the Rosaceae family. It has a delicious taste, high nutritional value and good economic benefits, and ranks first in the world's small berry production. The diseases that have a greater impact on strawberry yield and fruit quality in production are mainly anthracnose, powdery mildew, gray mold and root rot. Among them, strawberry powdery mildew can be caused by infection with Sphaerotheca aphanis. Strawberry powdery mildew is a low-temperature and high-humidity disease. The fungus produces a large amount of spores and re-infection is frequent, and the damage is getting worse year by year.

[0003] Chemical pesticides have the advantages of wide control range, convenient application and quick effect, which is why chemical control is still the main method for controlling strawberry powdery mildew in production. However, due to the unscientific application of chemical pesticides for a long time, large area and large dose in production, it has caused great selection pressure on pathogens, resulting in reduced sensitivity of pathogens to various existing chemical agents and different degrees of drug resistance, making the control effect less than ideal and easy to cause pesticide residues. Therefore, seeking new agents for controlling strawberry powdery mildew is of great significance to the green and healthy development of the strawberry industry.

[0004] Compounding different active ingredients is an effective and quick way to develop and research new pesticides and control resistant pathogens in agriculture. Compared with single active ingredients, compound combinations with synergistic effects can significantly improve the actual control effect and reduce the risk of resistance, which helps to reduce pesticide residues and control costs, and is currently an important means of integrated disease control.

[0005] At present, there are no reports on the combination of cyproconazole with bifenthionil or eugenol.

[0006] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0007] The object of the present invention is to provide a pesticide composition for preventing and controlling strawberry powdery mildew, so as to solve the problems existing when cyanobacterium methyl is used alone to prevent and control strawberry powdery mildew.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A pesticide composition for preventing and controlling strawberry powdery mildew, wherein the active ingredient is prepared by compounding cyproconazole and bixafenthrin or eugenol in a mass ratio of 1-200:200-1.

[0010] Furthermore, the mass ratio of cyanocobalamin to bixafen is 1-24:12-1.

[0011] Furthermore, the mass ratio of cyanocobalamin to eugenol is 1-15:15-1.

[0012] The present invention also provides a fungicide containing the active ingredient, wherein the mass of the active ingredient accounts for 0.75-60% of the total mass of the fungicide, and the remainder is auxiliary ingredients.

[0013] Furthermore, the auxiliary ingredients are selected from a variety of wetting agents, dispersants, dispersants, disintegrants, inert fillers, solvents, cosolvents, film formers, emulsifiers, thickeners, antifreeze agents, defoamers and warning colors.

[0014] The invention also provides application of the fungicide in preventing and controlling strawberry powdery mildew.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention compounds cyanobacteryl with bixafen or eugenol. The compound combination has an obvious synergistic effect on Monocystis niger that causes strawberry powdery mildew within a certain mass ratio range. Compared with any bactericidal active ingredient in the compound combination, the control effect on strawberry powdery mildew can be improved, and the risk of drug resistance when using a single bactericidal active ingredient can be reduced, which is helpful to delay the generation and accumulation of drug resistance of pathogens, reduce pesticide residues and control costs, and can provide support for the development and research of new agents for controlling strawberry powdery mildew. It is of great significance to the green and healthy development of the strawberry industry. DETAILED DESCRIPTION

[0017] The following is a clear and complete description of the technical solution of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Example: Indoor toxicity assay

[0019] 1. Test crops

[0020] Plant the red strawberry seedlings in pots until they reach the four-leaf, one-heart stage, one plant per pot, and number them for later use.

[0021] 2. Test spore suspension

[0022] Conidia of strawberry powdery mildew fungus Sphaerotheca aphanis were propagated on strawberry leaves. Fresh spores were washed with 0.05% Tween-80 aqueous solution, filtered with double gauze, and the concentration of spore suspension was adjusted to 1.0×10 6 pcs / mL, for future use.

[0023] 3. Test reagents

[0024] 95% cyproconazole technical (Jiangsu Pesticide Research Institute Co., Ltd.), 99% bifenthrin technical (Shanghai Titan Technology Co., Ltd.), 98.5% eugenol technical (Shanghai Yuanye Biotechnology Co., Ltd.).

[0025] The test drug was first dissolved in dimethyl sulfoxide, and then diluted with 0.05% Tween-80 aqueous solution to form a single-dose stock solution. Multiple groups of proportions were set, and each single dose and proportioned mixture was set with 5 mass concentration gradients according to the equal ratio method.

[0026] 4. Test methods

[0027] The spore suspension was sprayed evenly on the leaves of the test crop, with an inoculation volume of 3 mL / plant. After 24 hours, the prepared liquid was sprayed evenly, with a liquid volume of 3 mL / plant. Each mass concentration of liquid was repeated 4 times, with 3 pots of test crops in each repeat, and the treatment of spraying an equal amount of 0.05% Tween-80 aqueous solution was used as a blank control. After the treatment, the potted strawberries were transferred to 23°C and 16L / 8D light conditions for cultivation.

[0028] After the blank control is fully diseased, a graded disease investigation is conducted. The grading method is to evaluate the percentage of the lesion area of ​​each leaf to the entire leaf area (see Table 1 for details), calculate the disease index, and use the disease index to calculate the prevention effect.

[0029] Table 1 Disease grade assessment criteria (Li Jing et al., 2014)

[0030]

[0031]

[0032] 5. Data Analysis

[0033] Perform regression analysis on the logarithmic value of fungicide concentration and the probability value of control effect to calculate the EC value of the fungicide against the target pathogen 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to the Sun Yunpei method, and the synergistic effect of the agent was evaluated. The results are shown in Table 2-3.

[0034] Table 2 The results of toxicity test of cyproconazole and bixafenthrin against strawberry powdery mildew

[0035]

[0036]

[0037] As shown in Table 2, within the mass ratio of 1-24:12-1, the co-toxicity coefficient of the compound combination of cyproconazole and bixafen to Monocystis graminis of strawberry powdery mildew is greater than 120, showing an obvious synergistic effect. This indicates that the compounding of cyproconazole and bixafen at a certain mass ratio in the present invention can improve the control effect of strawberry powdery mildew.

[0038] Table 3 The results of toxicity test of cyanothiocarb and eugenol complex against strawberry powdery mildew

[0039]

[0040] As shown in Table 3, within the mass ratio of 1-15:15-1, the co-toxicity coefficient of the compound combination of cyanobacterium and eugenol to Monotheca graminis of strawberry powdery mildew is greater than 120, showing an obvious synergistic effect. This indicates that the compounding of cyanobacterium and eugenol in a certain mass ratio in the present invention can improve the control effect of strawberry powdery mildew.

[0041] In summary, the present invention compounds cyanocobalamin with bixafen or eugenol, and the compound combination has an obvious synergistic effect on Monocystis niger that causes strawberry powdery mildew within a certain mass ratio range, and can improve the control effect on strawberry powdery mildew compared with any fungicidal active ingredient in the compound combination.

Claims

1. A pesticide composition for preventing and controlling strawberry powdery mildew, characterized in that: The active ingredient is prepared by mixing cyanocobalamin and bixafenac or eugenol in a mass ratio of 1-200:200-1.

2. The pesticide composition for preventing and controlling strawberry powdery mildew according to claim 1, characterized in that: The mass ratio of cyanocobalamin to bixafen is 1-24:12-1.

3. The pesticide composition for controlling strawberry powdery mildew according to claim 1, characterized in that: The mass ratio of cyanocobalamin to eugenol is 1-15:15-1.

4. A fungicide containing the active ingredient according to any one of claims 1 to 3, characterized in that: The mass of the active ingredient accounts for 0.75-60% of the total mass of the fungicide, and the remainder is auxiliary ingredients.

5. The bactericide according to claim 4, characterized in that The auxiliary ingredients are selected from a variety of wetting agents, dispersants, dispersants, disintegrants, inert fillers, solvents, cosolvents, film formers, emulsifiers, thickeners, antifreeze agents, defoamers and warning colors.

6. Use of the fungicide according to claim 4 in preventing and controlling strawberry powdery mildew.

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