Fermented feed prepared from residual food on dining table and application of fermented feed

Abstract

The invention relates to a fermented feed prepared from residual food on a dining table and application of the fermented feed, and belongs to strain fermentation. The fermented feed is prepared by the following steps: firstly, collecting food remaining on a dining table, cleaning, deoiling and chopping, cutting residues into small blocks, performing high-temperature sterilization, and then adjusting the water content to 70%; and then, adopting a compound fermentation solution formed by mixing lactobacillus plantarum HS-1, saliva combined lactobacillus CICC 23174 and plant lactobacillus CICC 6002 according to a ratio of 1: 1: 1, adding the compound fermentation solution with the viable bacteria concentration of 1.2 * 10 < 9 > CFU / mL into the remaining food on the dining table according to an inoculum size of 5%, and carrying out closed fermentation at 37 DEG C for 24 hours to prepare the fermented feed. The prepared fermented feed can significantly promote the growth of the broiler chickens, enhance the activity of digestive enzymes and improve the immunity of the broiler chickens.

Description

Technical Field

[0001] The invention belongs to the technical field of strain fermentation, and in particular relates to a fermented feed made from table waste and application thereof. Background Art

[0002] With the growth of global population and the increase of food consumption, the disposal of table waste has become a global problem. Table waste is rich in organic matter, such as carbohydrates, proteins, fats and various trace elements. If it is directly discarded, it will not only waste resources, but also cause a serious burden on the environment. Traditional treatment methods such as landfill and incineration not only fail to effectively recycle resources, but also lead to greenhouse gas emissions and waste of land resources.

[0003] In this context, how to transform table waste into valuable products has become a hot topic of research. Fermentation technology, as a green and sustainable biological treatment method, has been widely used in agriculture and animal husbandry. Through microbial fermentation, table waste can be transformed into nutrient-rich fermented feed, which not only improves the reuse rate of waste food, but also reduces feed costs and solves the problem of resource waste.

[0004] At present, the main microorganisms used in fermented feed include lactic acid bacteria, yeast and bifidobacteria. These microorganisms can not only produce organic acids, enzymes and antibacterial substances during the fermentation process, but also inhibit the growth of harmful microorganisms, thereby improving the shelf life and safety of feed. However, how to select suitable strains for efficient fermentation and how to optimize fermentation conditions to ensure the nutrition of feed are the difficulties in current research and application. Summary of the invention

[0005] The purpose of the present invention is to provide a fermented feed made from table waste and application thereof, thereby realizing the reuse of table waste.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] First, the present invention provides a fermented feed made from table waste, wherein the fermented feed is prepared by the following steps:

[0008] (1) Collecting leftover food from the table, cleaning and degreasing it, and filtering it to obtain food residues;

[0009] (2) Chop table scraps into small pieces of 1-5 mm;

[0010] (3) sterilizing the chopped food residues by high pressure;

[0011] (4) Adjust the water content of the remaining food on the table after sterilization to 70% and stir evenly;

[0012] (5) The composite fermentation broth of the three strains was mixed in a ratio of 1:1:1, with a live bacteria concentration of 1.2×10^9 CFU / mL, and 5% of the composite fermentation broth was inoculated into the remaining food on the table, and the mixture was fermented in a closed manner at 37°C for 24 hours to obtain fermented feed;

[0013] The three strains are Lactobacillus plantarum HS-1, Lactobacillus salivarius CICC 23174 and Lactobacillus plantarum CICC 6002.

[0014] Preferably, the deposit number of the Lactobacillus plantarum HS-1 is CGMCC NO.21670.

[0015] Preferably, the fermented feed is used to increase the activity of digestive enzymes in the duodenum of broiler chickens, thereby accelerating the growth of broiler chickens.

[0016] Preferably, the digestive enzymes are amylase and trypsin, and the broiler chicken is Arbor Acres broiler chicken.

[0017] Secondly, the present invention provides a feed composition for improving the growth, immunity and digestion ability of broilers, wherein the feed composition is composed of 85% of a basic diet and 15% of the fermented feed according to any one of claims 1-4.

[0018] Preferably, the improving of immunity is to increase the content of immunoglobulin A and immunoglobulin G in broiler serum;

[0019] The improving digestibility is to improve the activities of amylase and trypsin in the duodenum of broiler chickens;

[0020] The broiler chickens are Arbor Acres broiler chickens.

[0021] Secondly, the present invention provides an application of a composite strain fermentation liquid in preparing a fermented feed for improving the growth of broilers, wherein the composite strain fermentation liquid consists of a Lactobacillus plantarum HS-1 fermentation liquid, a Lactobacillus salivae CICC 23174 fermentation liquid and a Lactobacillus plantarum CICC 6002 fermentation liquid;

[0022] In the composite strain fermentation liquid, the ratio of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC 23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid is 1:1:1.

[0023] Preferably, the live bacteria concentration of the composite strain fermentation broth used in preparing fermented feed is 1.2×10^9 CFU / mL, and the broilers are Arbor Acres broilers.

[0024] Secondly, the present invention provides an application of a composite strain fermentation liquid in preparing a fermented feed for improving the digestive enzyme activity of broilers, wherein the composite strain fermentation liquid consists of a Lactobacillus plantarum HS-1 fermentation liquid, a Lactobacillus salivae CICC 23174 fermentation liquid and a Lactobacillus plantarum CICC 6002 fermentation liquid;

[0025] In the composite strain fermentation liquid, the ratio of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC 23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid is 1:1:1.

[0026] Preferably, the live bacteria concentration of the composite strain fermentation liquid used in preparing fermented feed is 1.2×10^9 CFU / mL;

[0027] The broiler digestive enzymes are broiler duodenal amylase and trypsin.

[0028] The beneficial effects of the present invention are:

[0029] 1. The present invention avoids the environmental pollution caused by traditional treatment methods (such as landfill or incineration) by making table waste into fermented feed, and at the same time realizes the resource recycling of table waste, which meets the requirements of sustainable development.

[0030] 2. The present invention adopts a composite strain combination of plant lactobacillus HS-1, salivary lactobacillus CICC 23174 and plant lactobacillus CICC 6002 in a ratio of 1:1:1. These three strains significantly improve the efficacy of fermented feed through synergy, and show a strong synergistic effect in promoting broiler growth and improving digestive enzyme activity. This synergy ensures that the three-strain combination can maximize their respective advantages, which is significantly higher than the effect of a single strain or a combination of two strains. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is the body weight of broilers in different feed treatment groups on day 1;

[0032] Figure 2 The body weight of broilers in different feed treatment groups at 42 days;

[0033] Figure 3 This is the result diagram of the effect of different feed treatment groups on the serum immunoglobulin A (IgA) content of broiler chickens;

[0034] Figure 4 This is the result diagram of the effect of different feed treatment groups on the serum immunoglobulin G (IgG) content of broiler chickens;

[0035] Figure 5 This is the result diagram of the effect of different feed treatment groups on lipase activity in the duodenum of broiler chickens;

[0036] Figure 6 This is the result diagram of the effect of different feed treatment groups on the amylase activity in the duodenum of broiler chickens;

[0037] Figure 7 This is a graph showing the effect of different feed treatment groups on trypsin activity in the duodenum of broiler chickens;

[0038] Figure 1-Figure 7 In the table, * indicates P < 0.05 (compared with the control group), ** indicates P < 0.01 (compared with the control group), and *** indicates P < 0.001 (compared with the control group);

[0039] # indicates P < 0.05 (compared with experimental group 1), ## indicates P < 0.01 (compared with experimental group 1), and ### indicates P < 0.001 (compared with experimental group 1). DETAILED DESCRIPTION

[0040] Now describe in detail various exemplary embodiments of the present invention, this detailed description should not be considered as limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing a particular embodiment, and are not used to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the range.

[0041] The plant lactobacillus HS-1 used in the present invention has a preservation number of CGMCC NO.21670, which is preserved in the China General Microbiological Culture Collection Administration Center and is owned by this laboratory. The salivary lactobacillus CICC 23174, strain preservation number: CICC 23174 and plant lactobacillus CICC 6002, strain preservation number: CICC 6002, are purchased from the China Industrial Microbiological Culture Collection Administration Center.

[0042] Example 1

[0043] Pre-fermentation treatment of table waste

[0044] 1. Put the collected table leftovers into the cleaning tank, add clean water to clean the grease, and filter the net to get the table leftovers;

[0045] 2. Use a chopper to crush table scraps into small pieces of 1-5 mm to increase the contact area of ​​microorganisms;

[0046] 4. Put the mixed food residues into a high temperature resistant container and sterilize them at 121℃ for 15 minutes;

[0047] 5. After measuring the water content, add sterile water to make its water content 70%, stir evenly, and place the remaining food ingredients on the table in a container for later use.

[0048] Example 2

[0049] Preparation of Lactobacillus plantarum HS-1 fermentation broth

[0050] 1. Prepare MRS medium: peptone 10.0 g, yeast extract 5.0 g, beef extract 10.0 g, glucose 5.0 g, diammonium hydrogen citrate 2.0 g, sodium acetate 5.0 g, K2HPO4 2.0 g, MgSO4·7H2O.58 g, MnSO4·H2O 0.25 g, Tween 80 1 mL, distilled water to 1.0 L, pH 6.8.

[0051] 2. Thaw the frozen Lactobacillus plantarum HS-1 in a 4°C refrigerator to room temperature;

[0052] 3. Inoculate Lactobacillus plantarum HS-1 into 50 ml of fresh MRS medium, place at 37 degrees Celsius, and culture until the OD600 value is higher than 0.8 to obtain seed solution;

[0053] 4. Inoculate the seed liquid into 1000 ml of fresh MRS medium and culture it at 37°C until the strain concentration is greater than 10^9 CFU / mL to obtain Lactobacillus plantarum HS-1 fermentation liquid for later use.

[0054] Example 3

[0055] Preparation of saliva combined with Lactobacillus CICC 23174 fermentation broth

[0056] 1. Prepare MRS medium: peptone 10.0 g, yeast extract 5.0 g, beef extract 10.0 g, glucose 5.0 g, diammonium hydrogen citrate 2.0 g, sodium acetate 5.0 g, K2HPO4 2.0 g, MgSO4·7H2O.58 g, MnSO4·H2O 0.25 g, Tween 80 1 mL, distilled water to 1.0 L, pH 6.8.

[0057] 2. Thaw the frozen Lactobacillus salivae CICC 23174 in a 4°C refrigerator to room temperature;

[0058] 3. Inoculate saliva-associated Lactobacillus CICC 23174 into 50 ml of fresh MRS medium, place at 37 degrees Celsius, and culture until the OD600 value is higher than 0.8 to obtain seed solution;

[0059] 4. Inoculate the seed liquid into 1000 ml of fresh MRS medium and culture it at 37°C until the strain concentration is greater than 10^9 CFU / mL. Fermentation broth of saliva combined with Lactobacillus CICC 23174 is set aside.

[0060] Example 4

[0061] Preparation of Lactobacillus plantarum CICC 6002 fermentation broth

[0062] 1. Prepare MRS medium: peptone 10.0 g, yeast extract 5.0 g, beef extract 10.0 g, glucose 5.0 g, diammonium hydrogen citrate 2.0 g, sodium acetate 5.0 g, K2HPO4 2.0 g, MgSO4·7H2O.58 g, MnSO4·H2O 0.25 g, Tween 80 1 mL, distilled water to 1.0 L, pH 6.8.

[0063] 2. Thaw the frozen Lactobacillus plantarum CICC 6002 in a 4°C refrigerator to room temperature;

[0064] 3. Inoculate Lactobacillus plantarum CICC 6002 into 50 ml of fresh MRS medium, place at 37 degrees Celsius, and culture until the OD600 value is higher than 0.8 to obtain seed solution;

[0065] 4. Inoculate the seed liquid into 1000 ml of fresh MRS medium and culture it at 37°C until the strain concentration is greater than 10^9 CFU / mL to obtain the Lactobacillus plantarum CICC 6002 fermentation liquid for later use.

[0066] Example 5

[0067] Preparation of fermented feed from table waste

[0068] 1. The fermentation liquid of Lactobacillus plantarum HS-1, the fermentation liquid of Lactobacillus plantarum CICC 23174 and the fermentation liquid of Lactobacillus plantarum CICC 6002 are mixed to obtain a composite fermentation liquid with a viable bacteria concentration of 1.2×10^9 CFU / mL, wherein in the composite fermentation liquid, the ratio of the viable bacteria counts of Lactobacillus plantarum HS-1, Lactobacillus plantarum CICC 23174 and Lactobacillus plantarum CICC 6002 is 1:1:1.

[0069] 2. Add the composite fermentation liquid to the table leftover food material obtained in Example 1 at an inoculation rate of 5%;

[0070] 3. Put it into the blender, stir at 300r / min for 40 minutes, make sure the fermentation liquid is completely and evenly mixed with the remaining food on the table, then add the mixture into the fermentation bucket;

[0071] After 24 hours of closed fermentation at 4.37℃, the fermented feed made from table waste was obtained and placed at 4℃.

[0072] Example 6

[0073] Only Lactobacillus plantarum HS-1 was added, and the live bacteria concentration of the fermentation broth was 1.2×10^9. The remaining steps were the same as those in Example 5.

[0074] Example 7

[0075] The live bacteria concentration of the composite fermentation broth was 1.2×10^9, the live bacteria count ratio of Lactobacillus plantarum HS-1 and saliva combined Lactobacillus CICC 23174 was 1:2, and Lactobacillus plantarum CICC 6002 was not added; the remaining steps were the same as in Example 5.

[0076] Example 8

[0077] The live bacteria concentration of the composite fermentation liquid is 1.2×10^9, the live bacteria count ratio of Lactobacillus plantarum HS-1 and Lactobacillus plantarum CICC 6002 is 1:2, and saliva combined with Lactobacillus CICC 23174 is not added; the remaining steps are the same as in Example 5.

[0078] Example 9

[0079] The live bacteria concentration of the composite fermentation broth is 1.2×10^9, and the saliva-associated Lactobacillus CICC 23174 is replaced by Lactobacillus plantarum HN-1 (stored in this laboratory, and the fermentation broth preparation method is the same as in Example 2), wherein in the composite fermentation broth, the live bacteria count ratio of Lactobacillus plantarum HS-1, Lactobacillus plantarum HN-1, and Lactobacillus plantarum CICC 6002 is 1:1:1, and the remaining steps are the same as in Example 5.

[0080] Example 10

[0081] The live bacteria concentration of the composite fermentation broth is 1.2×10^9, and Lactobacillus plantarum CICC 6002 is replaced by Enterococcus faecium KDN-1 (stored in this laboratory, and the fermentation broth preparation method is the same as in Example 2), wherein in the composite fermentation broth, the live bacteria count ratio of Lactobacillus plantarum HS-1, Lactobacillus salivarius CICC 23174, and Enterococcus faecium KDN-1 is 1:1:1.

[0082] Embodiment 11

[0083] Testing the effects of different table waste fermentation feeds on broiler body weight

[0084] 1. One-day-old Arbor Acres broiler chickens (AA broiler chickens) were evenly divided into 7 groups, each group had 30 chickens, the control group was fed with a basal diet, the experimental group 1 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 5, the experimental group 2 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 6, the experimental group 3 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 7, the experimental group 4 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 8, the experimental group 5 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 9, and the experimental group 6 was fed with 85% of the basal diet + 15% of the fermented table leftovers obtained in Example 10;

[0085] The basic diet used in the present invention is shown in Table 1:

[0086] Table 1 Basic diet composition

[0087] project content corn 65.85 Soybean meal (46%) 16.14 Cottonseed meal (46%) 6.00 Corn gluten meal (60%) 4.00 Soybean Oil 3.17 Calcium Hydrogen Phosphate 1.64 Stone powder 1.05 Lysine (70%) 0.64 Choline chloride (50%) 0.10 baking soda 0.20 salt 0.21 Premix 1.00 total 100

[0088] Among them, the premix provides the following per kilogram of feed: VA, 10000IU; VD3, 2500IU; VE, 46mg; VK3, 2.50mg; VB1, 5.60mg; VB2, 11mg; VB6, 8mg; VB12, 10.5μg; niacin, 60mg; pantothenic acid, 32mg; folic acid, 2mg; biotin, 22.4μg; Fe, 80mg; Cu, 10mg; Mn, 60mg; Zn, 35mg; Co, 0.24mg; I, 0.42mg; Se, 0.35mg.

[0089] 2. Except for the difference in feed, other feeding conditions were the same. Ten rats were randomly selected from each group and weighed on the 1st and 42nd day. The results are shown in Table 2 and Table 3. Figure 1 Figure 2 shown.

[0090] Table 2 Differences in broiler weight after feeding with different feeds

[0091]

[0092] From the data in Table 2, it can be seen that at 1 day, there was no significant difference in the weight of AA broilers in each treatment group, indicating that the initial grouping was well balanced. At 42 days, the weight of broilers in the control group was 2034.28g, while the weight of broilers in all experimental groups was higher than that in the control group, and the P values ​​were all less than 0.001, indicating that these differences were significant. This further proves that fermented feed has a significant promoting effect on the growth of broilers.

[0093] Comparison of the experimental groups shows that:

[0094] Experimental group 1: At 42 days, the average weight of broilers reached 2309.41g, a significant increase of 275.13g compared with the control group. This shows that the composite fermentation liquid containing three strains (Lactobacillus plantarum HS-1, Lactobacillus salivarius CICC 23174, and Lactobacillus plantarum CICC6002) and a live bacteria concentration of 1.2×10^9 CFU / mL has a significant effect on the growth of broilers and is the best among all experimental groups.

[0095] Experimental group 2: The average weight of broilers at 42 days was 2149.05g, 114.77g higher than that of the control group. In the case of using a single strain fermented feed, although the weight increased, the effect was weaker than that of experimental group 1. This shows that the fermented feed of a single strain does not promote the growth of broilers as well as the fermented feed of a compound strain.

[0096] Experimental Group 3 and Experimental Group 4: The average weight of broilers at 42 days was 2184.33g and 2180.59g, respectively, both of which were higher than that of Experimental Group 2 with a single strain, but still significantly different from that of Experimental Group 1. This shows that the combination of two strains is more effective than a single strain, but not enough to achieve the effect of a combination of three strains.

[0097] Experimental Group 5 and Experimental Group 6: The average weight of broilers at 42 days was 2200.82g and 2216.31g, respectively. Although the weight of experimental group 5 was higher than that of experimental group 3, the difference was not statistically significant, indicating that the effect of the combination of three strains may not always be better than the combination of two strains. The weight of broilers in experimental group 6 was significantly higher than that in experimental group 3, and the difference was statistically significant, but there was still a significant difference compared with experimental group 1. This shows that although Enterococcus faecalis KDN-1 has a certain effect in promoting the growth of broilers, it fails to achieve the effect in experimental group 1. This shows that the role of Lactobacillus plantarum CICC 6002 in the composite fermentation broth has a significant synergistic effect, while Enterococcus faecalis fails to produce the same synergistic effect.

[0098] From the above results, it can be seen that the combination of plant lactobacillus HS-1, salivary lactobacillus CICC 23174 and plant lactobacillus CICC 6002 in a ratio of 1:1:1 for the fermentation of table waste has a significant synergistic effect, and the resulting fermented feed can significantly promote the growth of broilers. However, the effect achieved by the above three strain combinations cannot be achieved by using a single strain, a combination of two strains, or replacing one of the strains. This shows that the three strains can work synergistically during the fermentation process, enhance the nutritional value of the feed and promote the growth of broilers.

[0099] Example 12

[0100] Testing the effects of feeding different types of table waste fermented feed on broiler immunity

[0101] On the 42nd day of the experiment, 5 mice were randomly selected from each group and fasted for 12 h. Two tubes of whole blood (5 ml each) were collected from the wing veins;

[0102] Centrifuge at 3000r / min for 15min to collect serum;

[0103] Serum immunoglobulin A (IgA) and immunoglobulin G (IgG) detection kits were used to detect IgA and IgG according to the instructions. The results are shown in Table 2 and Figure 3 and Figure 4 shown.

[0104] Table 2 Effects of feeding different table waste fermented feed on broiler immunity

[0105]

[0106] Combined with Table 2, Figure 3 and Figure 4 The data show that compared with the control group, the levels of IgA and IgG in the serum of broiler chickens in the experimental group increased, and the difference was statistically significant (P<0.05). This shows that different fermented feeds have a significant effect on enhancing the immune system of broiler chickens, and fermented feeds can improve the humoral immune response of broiler chickens.

[0107] However, although the levels of IgA and IgG in the serum of broiler chickens in experimental group 1 were the highest and statistically different from those in experimental groups 2, 3, and 4, only IgG had statistical differences compared with experimental groups 5 and 6, while IgG had no statistical differences.

[0108] From the above results, it can be seen that although the three strains of Lactobacillus plantarum HS-1, Lactobacillus salivae CICC 23174 and Lactobacillus plantarum CICC 6002 were used in a 1:1:1 combination for the fermentation of table waste feed, the three strains had certain advantages in some immune parameters, but the difference was not significant. This may be due to the functional overlap between different strains in regulating immune responses, or the interaction between strains did not fully exert the synergistic effect. In general, the three-strain fermented feed can improve the immune capacity of broilers, but its synergistic effect in immunoglobulin is not significant, especially in the improvement of IgA, which does not show obvious advantages.

[0109] Example 13

[0110] Examining the effects of feeding different types of table waste fermented feed on digestive enzymes in broiler chickens

[0111] On the 42nd day of the experiment, 5 roosters were randomly selected from each group, and their duodenums were taken after slaughter. The chyme in them was collected into cryopreservation tubes and stored in liquid nitrogen;

[0112] The frozen chyme samples were thawed and added with normal saline in a ratio of 1:1 to prepare a homogenate;

[0113] After centrifugation at 4000r / min and 4℃ for 10min, the supernatant was taken and the lipase kit, trypsin kit and amylase kit were used to detect the differences in lipase, trypsin and amylase in the duodenum of different treatment groups. The results are shown in Table 3 and Figure 5 , Figure 6 , Figure 7 shown.

[0114] Table 3 Effects of feeding different table waste fermented feeds on duodenal digestive enzymes in broiler chickens

[0115]

[0116] From the results of lipase activity, it can be seen that after feeding different fermented feeds, only the lipase activity of the duodenum of broiler chickens in experimental group 1 showed statistically significant differences, but the difference was small. This shows that the fermented feed of table waste fermented with different strains has basically no significant effect on the lipase activity in the duodenum of broiler chickens. This shows that the direct effect of fermented feed on lipase is relatively limited.

[0117] From the results of amylase and trypsin, it can be seen that the amylase and trypsin activities of experimental group 1 were the strongest, increasing by 71.65% and 100.52% respectively compared with the control group. In contrast, experimental group 2 only increased by 7.87% and 18.80% respectively, experimental group 3 increased by 16.54% and 25.80% respectively, experimental group 4 increased by 15.75% and 27.48% respectively, while experimental group 5 increased by 29.92% and 38.91%, and experimental group 6 increased by 35.43% and 51.92%. These data show that the three-strain combination of experimental group 1 has the most significant effect on promoting amylase and trypsin.

[0118] Compared with experimental group 1, the effect of feed fermented with Lactobacillus plantarum HS-1 alone (experimental group 2) on improving the activity of amylase and trypsin in the duodenum of broiler chickens was significantly reduced. When Lactobacillus plantarum HS-1 was fermented with other strains (such as Lactobacillus salivarius CICC 23174 or Lactobacillus plantarum CICC 6002), although the effect was improved (experimental groups 3 and 4), it was still not as good as the effect of the three-strain combination (experimental group 1).

[0119] In addition, after replacing saliva with Lactobacillus CICC 23174 or Lactobacillus plantarum CICC 6002 (such as experimental groups 5 and 6), although these combinations are better than single-strain fermented feed (experimental group 2) and double-strain combination (experimental group 3 and experimental group 4), the effect of promoting the activity of amylase and trypsin is still significantly lower than the combined fermented feed of three strains in Example 1 (experimental group 1). This result shows that in experimental groups 5 and 6, although the feed is also obtained by fermentation of the three-strain combination, due to the replacement of some strains, the synergistic effect of the three strains in experimental group 1 in promoting the activity of digestive enzymes cannot be fully achieved.

[0120] From the above results, it can be seen that only when Lactobacillus plantarum HS-1, Lactobacillus salivae CICC 23174 and Lactobacillus plantarum CICC 6002 are combined in a 1:1:1 ratio for table waste fermentation can the amylase and trypsin activities in the duodenum of broilers be significantly improved. Although experimental groups 5 and 6 also used three-strain fermentation, the effect was significantly reduced compared with experimental group 1 due to the influence of strain ratio or strain replacement. This shows that the ratio of the three-strain combination and the selection of specific strains are crucial to the digestive enzyme-promoting effect of fermented feed. Reasonable strain matching can significantly enhance the digestive ability of broilers, thereby improving growth effects.

Claims

1. A fermented feed made from table waste, characterized in that: The fermented feed is prepared by the following steps: (1) Collecting leftover food from the table, cleaning and degreasing it, and filtering it to obtain food residues; (2) Chop table scraps into small pieces of 1-5 mm; (3) sterilizing the chopped food residues by high pressure; (4) Adjust the water content of the remaining food on the table after sterilization to 70% and stir evenly; (5) The composite fermentation broth of the three strains was mixed in a ratio of 1:1:1, with a live bacteria concentration of 1.2×10^9 CFU / mL, and 5% of the composite fermentation broth was inoculated into the remaining food on the table, and the mixture was fermented in a closed manner at 37°C for 24 hours to obtain fermented feed; The three strains are Lactobacillus plantarum HS-1, Lactobacillus salivarius CICC 23174 and Lactobacillus plantarum CICC6002.

2. The fermented feed according to claim 1, characterized in that: The deposit number of the Lactobacillus plantarum HS-1 is CGMCC NO.21670.

3. The fermented feed according to claim 2, characterized in that: The fermented feed is used to increase the activity of digestive enzymes in the duodenum of broiler chickens, thereby accelerating the growth of broiler chickens.

4. The fermented feed according to claim 3, characterized in that: The digestive enzymes are amylase and trypsin, and the broiler chicken is Arbor Acres broiler chicken.

5. A feed composition for improving the growth, immunity and digestion ability of broiler chickens, the feed composition comprising 85% of a basic diet and 15% of the fermented feed according to any one of claims 1 to 4.

6. The feed composition according to claim 5, characterized in that The improving of immunity is to increase the content of immunoglobulin A and immunoglobulin G in broiler serum; The improving digestibility is to improve the activities of amylase and trypsin in the duodenum of broiler chickens; The broiler chickens are Arbor Acres broiler chickens.

7. Application of a composite strain fermentation liquid in preparing fermented feed for improving broiler growth, characterized in that: The composite bacterial strain fermentation liquid is composed of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid; In the composite strain fermentation liquid, the ratio of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC 23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid is 1:1:

1.

8. The use according to claim 7, characterized in that: The live bacteria concentration of the composite strain fermentation liquid used in preparing fermented feed is 1.2×10^9 CFU / mL, and the broilers are Arbor Acres broilers.

9. Application of a composite strain fermentation liquid in preparing a fermented feed for improving the digestive enzyme activity of broiler chickens, characterized in that: The composite bacterial strain fermentation liquid consists of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC 23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid; In the composite strain fermentation liquid, the ratio of Lactobacillus plantarum HS-1 fermentation liquid, Lactobacillus salivae CICC 23174 fermentation liquid and Lactobacillus plantarum CICC 6002 fermentation liquid is 1:1:

1.

10. The use according to claim 9, characterized in that: The live bacteria concentration of the composite strain fermentation liquid used in preparing fermented feed is 1.2×10^9 CFU / mL; The broiler digestive enzymes are broiler duodenal amylase and trypsin.

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