Multifunctional pet nutrition cream and preparation method thereof

Abstract

The present invention relates to a multifunctional pet nutrition cream and a preparation method thereof, and belongs to the technical field of pet nutrition agents, and the multifunctional pet nutrition cream comprises the following components in parts by weight: 12-27 parts of elaeagnus angustifolia fruit powder, 4-13 parts of a rubus corchorifolius water extract, 45-50 parts of freeze-dried meat powder, 8-15 parts of starch, 5 parts of maltitol, 0.5 part of vitamins, 0.5 part of minerals, 3-7 parts of glycerol, 3 parts of an emulsifier, and the balance of vegetable oil, totaling 100 parts. The multifunctional pet nutrition cream can be used as a daily basic additional supplement nourishment, can obviously relieve fatigue, has a certain anti-oxidation effect, and has a certain antidiarrheal effect when being taken at a special dosage.

Description

Technical Field

[0001] The invention belongs to the technical field of pet nutritional agents and relates to a multifunctional pet nutritional paste and a preparation method thereof. Background Art

[0002] With economic growth and the improvement of people's living standards, more and more people are beginning to pay attention to the quality of their spiritual life. Pets, as human animal companions and partners, can provide people with emotional comfort and sustenance, improve the quality of people's spiritual life, and are subsequently loved by more and more families.

[0003] Pet cats and dogs are loved by most people due to their appearance and personality characteristics, and have become the most common types of pets in daily life. Unlike the breeding of other livestock and poultry, people pay more attention to the health and life span of pets in the process of raising pets. Daily nutrition is necessary for the growth, development, production, and reproduction of pets. Each nutrient plays a positive role in the pet body. Both lack and excess of nutrition will have a negative impact on the health of pets.

[0004] Currently, the fat content of pet nutritional pastes on the market is relatively high. This type of high-fat nutritional paste has good palatability and is easy to preserve. However, due to the high fat content of current paste products, it is not only easy to cause stratification and precipitation, affecting the product's appearance and the loss of nutrients; it can also cause problems such as excessive calorie intake and nutrient indigestion in pets.

[0005] Moreover, the pet nutrition cream products on the market generally only provide daily nutrition and have a single function. Therefore, it is very necessary to develop a multifunctional pet nutrition cream. Summary of the invention

[0006] The invention aims to provide a multifunctional pet nutritional paste and a preparation method thereof.

[0007] The technical solution adopted by the present invention to achieve its purpose is as follows: A multifunctional pet nutritional paste, characterized in that, by weight, it includes 12-27 parts of Elaeagnus angustifolia fruit powder, 4-13 parts of Rubus sakhalinensis water extract, 45-50 parts of freeze-dried meat powder, 8-15 parts of starch, 5 parts of maltitol, 0.5 parts of vitamins, 0.5 parts of minerals, 3-7 parts of glycerol, 3 parts of emulsifier, and vegetable oil supplemented to 100 parts.

[0008] Preferably, the protein content of the freeze-dried meat powder is greater than 80%, and the freeze-dried meat powder is obtained by crushing the freeze-dried meat, passing it through a 40-mesh sieve, and irradiating it.

[0009] Preferably, the freeze-dried meat powder includes 0-20 parts of chicken liver powder, 20-30 parts of chicken breast powder, 0-20 parts of beef dry powder, and 0-5 parts of fish dry powder.

[0010] Preferably, the vegetable oil is one or any combination of linseed oil, soybean oil and coconut oil.

[0011] Preferably, the elata fruit powder is obtained by grinding elata fruit, for example: 500 grams of mature elata fruit are taken, after impurity removal and cleaning, they are dried in a hot air circulation oven to ensure uniform drying and retain active ingredients; the dried elata is coarsely crushed, and a 50-mesh sieve is used for initial screening to obtain about 400 grams of elata fine powder; the elata fine powder is evenly fed into the feed port of a WDJ-250 ultrafine pulverizer, the speed is set to 25,000 rpm for crushing, and separated through a 300-mesh sieve to obtain about 350 grams of ultrafine powder that meets the requirements, and finally the elata fruit ultrafine powder product is sealed and packaged, and stored in a dry, light-proof environment for later use.

[0012] Preferably, the water extract of Rubus sakhalinensis is prepared by the following method: Weigh 100 g of Rubus sakhalinensis into a 1000 mL round-bottom flask, add 150 mL of petroleum ether, and reflux for degreasing at 70°C for 2 times, each time for 2 hours; after evaporating the solvent from the residue, add 150 mL of distilled water, reflux for extraction 3 times, each time for 2 hours, combine the filtrates, and concentrate under reduced pressure to a solution concentration of 1 g / mL to obtain the Rubus sakhalinensis water extract.

[0013] Preferably, when the multifunctional pet nutritional paste is fed to pet cats, the mass ratio of Elaeagnus angustifolia fruit powder to Rubus sakhalinensis water extract is controlled to be 3:1; when the multifunctional pet nutritional paste is fed to pet dogs, the mass ratio of Elaeagnus angustifolia fruit powder to Rubus sakhalinensis water extract is controlled to be 3:(2-2.5).

[0014] A method for preparing a multifunctional pet nutritional paste comprises the following steps: S1. The elata fruit powder, Rubus sakhalinensis water extract, freeze-dried meat powder, starch, maltitol, vitamins and minerals are mixed to obtain a premix; S2. Add glycerol, vegetable oil and emulsifier to the premix obtained in step S1, stirring while adding to obtain a mixture; S3. Filling to obtain the multifunctional pet nutritional paste.

[0015] Preferably, in step S2, the stirring temperature is 20-28° C. and the stirring time is 10 min.

[0016] Preferably, in step S3, during filling, the water content is controlled to be lower than 3%.

[0017] The beneficial effects of the present invention are: The multifunctional pet nutritional paste of the present invention can not only be used as a daily basic additional nutritional supplement, but also can significantly reduce and relieve fatigue, has a certain anti-oxidation effect, and also has a certain anti-diarrhea effect when taken in a special dose. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is the scavenging rate of DPPH free radicals in antioxidant experiment.

[0019] Figure 2 It is the ABTS free radical scavenging rate of antioxidant experiment. DETAILED DESCRIPTION

[0020] The present invention is described in detail below in conjunction with specific embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should belong to the scope of protection of the present invention.

[0021] 1. Example Table 1 Note: Vitamins are any mixture of vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, and vitamin K; minerals are any mixture of calcium, iron, zinc, iodine, and selenium.

[0022] 2. Experimental results 2.1 Palatability test Examples 1-3 used pet cats as experimental subjects, with 30 cats in each group; Examples 4-6 used pet dogs as experimental subjects, with 30 cats in each group. See Table 2 for palatability results.

[0023] Table 2 2.2 Anti-fatigue test 2.2.1 Experimental drugs Example 1 Nutrient cream, distilled water, and physiological saline.

[0024] 2.2.2 Experimental animals Twenty ICR mice were provided by the Animal Center of Xinjiang Medical University.

[0025] 2.2.3 Experimental methods 16 ICR mice, half male and half female, weighing 20g±2g, were randomly divided into 4 groups, 4 mice each, 3 groups of multifunctional pet nutrition paste groups (high, medium and low), and 1 group of blank control group (normal saline). They were placed in corresponding cages.

[0026] Mice were given oral administration in high, medium and low dose groups, respectively, with the low dose being 0.1g / ml, the medium dose being 0.2g / ml and the high dose being 0.4g / ml. The blank control group was given the same volume of saline. Each mouse was given 0.2ml / 10g of medication, and after oral administration, the mice were allowed to wait for 30 minutes, and the speed of the roller was adjusted to 18 revolutions / min, so that the mice began to pedal the wheel. The mice were allowed to adapt to the roller first to avoid experimental errors, and then the number of rotations of the wheel was measured when the mice fell off the wheel due to muscle fatigue.

[0027] Experimental data calculation: number of rotations before drug administration - number of rotations after drug administration. 2.2.4 Data Analysis The results of the mouse wheel experiment can be seen in Table 3. As the content of the multifunctional pet nutritional paste increases, the number of times the mice fall off the wheel after taking the medicine is significantly reduced compared with the number of times before taking the medicine. The difference between the blank group before and after is basically small. The three groups of data are generally positive compared with the saline group. Among them, the data of the low-dose group is relatively unstable compared with the medium-dose and high-dose groups. It can be seen that the number of times the mice fall off the wheel is the largest among the three groups of data. The analysis is due to the low content of the low dose and some mice are not well adapted to the wheel. The data of the medium and high doses are relatively stable, and the number of times the mice fall off the wheel after taking the medicine in two groups is 0. The calculation using SPSS software shows that p < 0.05, indicating that the nutritional paste of Example 1 has an anti-fatigue effect on mice and is relatively stable.

[0028] Table 3 Table 4 Significant difference (medium dose group) Table 5 Significant difference (high dose group) 2.3 Antidiarrhea Experiment Antidiarrhea experimental process: The experimental animals were provided by the Animal Experiment Center of Xinjiang Medical University, and the experiments were approved by the Experimental Animal Ethics Committee of Xinjiang Medical University.

[0029] Preparation of senna suspension: Remove the veins of the dried senna leaves and grind them into fine powder. Weigh 8 g and put it into a beaker, add water and boil. Filter out the residue. After cooling, add distilled water to make up to 100 mL to prepare 8% senna suspension for later use.

[0030] Effects of senna on diarrhea in mice: After fasting for 12 h, the drug groups were gavaged with drugs, the model group and the blank control group were given the same volume of normal saline, and the positive control group was given montmorillonite powder solution. One hour after the last drug administration, the blank control group was not given any drug, and the other groups were gavaged with 8% senna suspension, with a dosage of 0.6 mL / mouse. The absorbent paper was changed once every hour within 4 h after drug administration, and the total number of wet feces in each group within 4 h was calculated.

[0031] Separate the mice into cages, one cage for each mouse, place filter paper under the cage, record the number of defecation, and observe the following indicators: Diarrhea stool: irregular shape, watery stool, bloody stool, etc.; Loose stool rate = number of loose stools ÷ total number of stools, with each pile / piece of stool on the filter paper being considered one defecation; Loose stool grade (diameter of loose stool contamination area): Grade 1 (<1 cm); Grade 2 (1-1.9 cm); Grade 3 (2-3 cm); Grade 4 (>3 cm); Average degree of loose stools: the sum of the degrees of each pile of loose stools divided by the number of loose stools; Diarrhea index = loose stool rate × average loose stool level.

[0032] Effects on normal small intestinal propulsion: After fasting for 12 h, each dose group was given 0.4 mL / 10 g by gavage, and the blank control group was given the same volume of normal saline; 30 min later, each group was given 0.4 mL / mouse of India ink, and the mice were anesthetized and killed 30 min later. The small intestine was dissected out and gently pulled into a straight line. The intestinal length was measured as the "total length of the small intestine", and the distance from the pylorus to the front of the charcoal end was measured as the ink advancement distance.

[0033] Ink advancement rate (%) = ink advancement distance in the intestine (cm) ÷ total length of small intestine (cm) × 100%.

[0034] Table 6 2.4 Antioxidant Experiment 2.4.1 Preparation of required solutions 2.4.1.1 Preparation of DPPH solution: Weigh 4.43 mg of DPPH into a 100 mL brown volumetric flask, dilute to volume with anhydrous ethanol, and fully dissolve. Store in a dark place for future use.

[0035] 2.4.1.2 Preparation of VC solution: Weigh 26.76 mg of VC into a 25 mL volumetric flask, add distilled water to the mark, and fully dissolve. Store in a dark place for later use.

[0036] 2.4.1.3 Preparation of ABTS solution: Weigh 36.02 mg of ABTS and 12.60 mg of K2S2O4, dilute to 10 mL with distilled water, mix the two, wrap with tin foil, let stand for 16 hours, then add anhydrous ethanol to dilute to an absorbance of 0.7±0.02. Store in a dark place for later use.

[0037] 2.4.2 Preparation of test solution Weigh each batch of nutrient paste (about 5 g in terms of Elaeagnus angustifolia fruit powder), add 70% ethanol at a solid-liquid ratio of 1:10, soak for 30 min, then ultrasonicate for 30 min, centrifuge at 3000 r / min for 15 min, and take the supernatant to obtain the test solution.

[0038] 2.4.3 Determination of DPPH free radical scavenging rate of alcohol extraction fraction The test solution obtained under 2.4.2 was diluted in sequence. Based on the Elaeagnus angustifolia fruit powder, solutions with concentrations of 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0 mg / mL were obtained. 2 mL of a series of sample solutions were taken into a test tube, and 2 mL of the DPPH solution prepared under 2.4.1.1 was added. The mixture was mixed and placed in a dark place to react for 30 min at room temperature. The absorbance was measured at a wavelength of 517 nm and recorded as A1. The absorbance was measured after 2 mL of the sample solution was fully mixed with 2 mL of anhydrous ethanol solution and recorded as A2. The absorbance was measured after 2 mL of distilled water was mixed with 2 mL of DPPH solution and recorded as A0. VC was used as the positive control and each sample was measured in parallel 3 times. The scavenging rate of the test solution on DPPH free radicals was calculated according to formula (1).

[0039] Clearance rate (%) = [1-(A1-A2) / A0] × 100% (1) The clearance results at different concentrations measured by the above method are as follows Figure 1 It can be seen that within the concentration range of 2.0-8.0 mg / mL, as the concentration of the sample solution increases, the scavenging rate of the test solution on DPPH free radicals gradually increases, showing a certain dose-effect relationship.

[0040] 2.4.4 Determination of ABTS free radical scavenging rate of alcohol extraction fraction Based on the Elaeagnus angustifolia fruit powder, the test solution was diluted in sequence to obtain solutions with concentrations of 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0 mg / mL. 0.2 mL of a series of sample solutions were added to test tubes, and 3.9 mL of the ABTS solution prepared under 2.4.1.3 was added thereto. Mix well, place in the dark at room temperature for 15 min, and measure the absorbance A1 at 734 nm; 0.2 mL of the sample solution was mixed with 3.9 mL of anhydrous ethanol solution, and the absorbance A2 was measured; 3.9 mL of ABTS solution was mixed with 0.2 mL of distilled water, and the absorbance A0 was measured. VC was used as the positive control, and each sample was measured in parallel 3 times. The ABTS free radical scavenging rate of the test solution was calculated according to formula (2).

[0041] Clearance rate (%) = [1-(A1-A2) / A0] × 100% (2) The clearance results at different concentrations measured by the above method are as follows Figure 2 It can be seen that within the concentration range of 2.0-8.0 mg / mL, as the concentration of the sample solution increases, the scavenging rate of the test solution on ABTS free radicals gradually increases, showing a certain dose-effect relationship.

Claims

1. A multifunctional pet nutritional cream, characterized in that: Calculated by weight, it includes 12-27 parts of Elaeagnus angustifolia fruit powder, 4-13 parts of Rubus sakhalinensis water extract, 45-50 parts of freeze-dried meat powder, 8-15 parts of starch, 5 parts of maltitol, 0.5 parts of vitamins, 0.5 parts of minerals, 3-7 parts of glycerol, 3 parts of emulsifier, and vegetable oil to make up to 100 parts.

2. A multifunctional pet nutrition paste according to claim 1, characterized in that: The protein content of the freeze-dried meat powder is greater than 80%, and passes through a 40-mesh sieve.

3. A multifunctional pet nutrition paste according to claim 1, characterized in that: The freeze-dried meat powder comprises 0-20 parts of chicken liver powder, 20-30 parts of chicken breast powder, 0-20 parts of beef dry powder and 0-5 parts of fish dry powder.

4. The multifunctional pet nutrition paste according to claim 1, characterized in that: The vegetable oil is one of linseed oil, soybean oil and coconut oil or any combination thereof.

5. The multifunctional pet nutrition paste according to claim 1, characterized in that: The Elaeagnus angustifolia fruit powder is obtained by pulverizing Elaeagnus angustifolia fruit, and the Elaeagnus angustifolia fruit powder is sieved through a 300-mesh sieve.

6. The multifunctional pet nutrition paste according to claim 1, characterized in that: The water extract of Rubus sakhalinensis is prepared by the following method: Weigh 100 g of Rubus sakhalinensis into a 1000 mL round-bottom flask, add 150 mL of petroleum ether, and reflux for degreasing at 70°C for 2 times, each time for 2 hours; after evaporating the solvent from the residue, add 150 mL of distilled water, reflux for extraction 3 times, each time for 2 hours, combine the filtrates, and concentrate under reduced pressure to a solution concentration of 1 g / mL to obtain the Rubus sakhalinensis water extract.

7. The multifunctional pet nutrition paste according to claim 1, characterized in that: The multifunctional pet nutritional paste is fed to pet cats, and the mass ratio of Elaeagnus angustifolia fruit powder to Rubus sakhalinus water extract is controlled to be 3:1; the multifunctional pet nutritional paste is fed to pet dogs, and the mass ratio of Elaeagnus angustifolia fruit powder to Rubus sakhalinus water extract is controlled to be 3:(2-2.5).

8. A method for preparing a multifunctional pet nutritional paste, characterized in that: The following steps are involved: S1. The elata fruit powder, Rubus sakhalinensis water extract, freeze-dried meat powder, starch, maltitol, vitamins and minerals are mixed to obtain a premix; S2. Add glycerol, vegetable oil and emulsifier to the premix obtained in step S1, stirring while adding to obtain a mixture; S3. Filling to obtain the multifunctional pet nutritional paste.

9. The method for preparing a multifunctional pet nutritional paste according to claim 8, characterized in that: In step S2, the stirring temperature is 20-28°C and the stirring time is 10 min.

10. The method for preparing a multifunctional pet nutritional paste according to claim 8, characterized in that: In step S3, during filling, the water content is controlled to be less than 3%.

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