Boessen berry composition with whitening function, beverage, preparation method and application

Abstract

The invention provides a boessen berry composition with a whitening function, and belongs to the technical field of whitening products, the effective components of the composition comprise concentrated boessen berry juice or powder and white onion powder or extract, and the weight ratio of the concentrated boessen berry juice or powder to the white onion powder or extract is (1-8.1): 1. The composition with the whitening function can achieve a good whitening effect, can remarkably improve the skin state of a human body, and is suitable for users of all ages. The drink mainly selects natural plant extracts as effective components, and has no side effect on human bodies. In addition, the invention further provides the boessen berry beverage with the whitening function, and a preparation method and application of the boessen berry beverage.

Description

Technical Field

[0001] The present application relates to the technical field of whitening products, and in particular to a boysenberry composition and beverage with whitening function, and a preparation method and use thereof. Background Art

[0002] As we age, our body's sugar intake accumulates. As our metabolism slows down, the sugar in our body easily accumulates, combines with proteins, oxidizes, and finally forms protein advanced glycation end products (AGEs), which cause skin aging and dull skin. At the same time, as we age, our skin becomes oxidized and becomes dull. Over time, it can cause our skin to become loose, aged, and wrinkled. In addition, ultraviolet rays can cause great damage to the skin and produce free radicals, which accelerate skin oxidation. The whitening effects of whitening products currently on the market are insufficient, and more effective whitening products are urgently needed. Summary of the invention

[0003] The purpose of the present application is to provide a boysenberry composition, a beverage, a preparation method and a use thereof with whitening function to improve the above-mentioned problems.

[0004] In a first aspect, an embodiment of the present application further provides a boysenberry composition with whitening function, wherein the active ingredients in the composition are composed of concentrated boysenberry juice or powder and white onion powder or extract, and the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 1-8.1:1.

[0005] In one embodiment, the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 2-8.1:1.

[0006] In a second aspect, an embodiment of the present application provides a boysenberry drink with whitening function, wherein the active ingredients in the drink are composed of concentrated boysenberry juice or powder, white onion powder or extract, and water, wherein the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 1-8.1:1, and the concentration of concentrated boysenberry juice or powder is 2-20 mg / ml.

[0007] In one embodiment, the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 2-8.1:1.

[0008] In one embodiment, the beverage further comprises an additive that can be added to food, and the additive is selected from one or more of collagen peptides, sodium hyaluronate, fruit juice, fruit powder, sugar alcohol, steviol glycoside, and edible flavors.

[0009] In a third aspect, an embodiment of the present application also provides a method for preparing the above-mentioned boysenberry drink with whitening function, which is obtained by uniformly mixing concentrated boysenberry juice or powder, white onion powder or extract and water, wherein the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 1-8.1:1.

[0010] In one embodiment, the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 2-8.1:1.

[0011] In one embodiment, the concentration of the white onion powder or extract is 2-20 mg / ml.

[0012] In one embodiment, the method comprises: adding an additive to a portion of water, heating and stirring until the colloid is swollen and then standing, uniformly mixing concentrated boysenberry juice or powder, white onion powder or extract and a portion of water, and then uniformly mixing with the additive solution system after standing; wherein the additive is selected from one or more of collagen peptides, sodium hyaluronate, fruit juice, fruit powder, sugar alcohol, steviol glycoside, and edible flavors.

[0013] In one embodiment, the concentration of the white onion powder or extract is 2-20 mg / ml.

[0014] In a fourth aspect, the embodiments of the present application also provide a use of the above-mentioned boysenberry composition with whitening function in the preparation of whitening drugs.

[0015] In a fifth aspect, the embodiments of the present application also provide a use of the above-mentioned boysenberry beverage with whitening function in the preparation of skin whitening medicines.

[0016] The boysenberry composition, beverage, preparation method and use with whitening function provided in the present application can achieve good whitening effect, can significantly improve the skin condition of human body, and is suitable for users of all ages. The beverage mainly uses natural plant extracts as active ingredients and has no side effects on human body.

[0017] These and other aspects of the present application will become more apparent from the description of the following embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 A typical graph of melanin signal intensity in the zebrafish head after sample treatment in Experimental Example 1 is shown.

[0020] Figure 2 The data graph shows the melanin signal intensity of the zebrafish head after sample treatment in Experimental Example 1 and the melanin signal intensity of the zebrafish head in the model control group.

[0021] Figure 3 The whitening efficacy data of each sample in Experimental Example 1 is shown. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0023] The formation of advanced glycation end products (AGEs) is mainly divided into three stages (initial stage, propagation stage and advanced stage). In the initial stage, the amino acids in the protein combine with the sugar molecules, and the non-enzymatic reaction between the aldehyde group of the sugar and the amino acid of the protein forms Schiff's base. In the propagation stage, the Schiff's base is further oxidized to form the glycosylation precursor methylglyoxal (MGO). Methylglyoxal has active oxygen free radicals, so in the advanced stage, methylglyoxal further generates advanced glycation end products (AGEs).

[0024] As one aspect of the present application, an embodiment of the present application provides a boysenberry composition with whitening function, wherein the effective ingredients of the composition are composed of concentrated boysenberry juice or powder and white onion powder or extract, and the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 1-8.1:1. For example, in some embodiments, the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract may be 1:1, 2:1, 3:1, 4:1, 5:1, 7:1, 8:1, etc. It should be noted that the effective ingredients in the composition may include only concentrated boysenberry juice or powder and white onion powder or extract.

[0025] The concentrated boysenberry juice or powder may refer to concentrated boysenberry juice or boysenberry powder or concentrated boysenberry powder.

[0026] Concentrated boysenberry juice may refer to an extract extracted from boysenberries. Boysenberries are rich in anthocyanins and polyphenols, vitamins, folic acid, trace elements, etc., and have the effects of anti-oxidation, nutritional supplementation, and immune enhancement. In some embodiments, concentrated boysenberry juice can be extracted in the following manner: fresh, mature, disease-free boysenberries are selected, washed, and crushed to break the fruit cell walls to facilitate the subsequent dissolution of effective ingredients. After crushing, further pulping is performed to form a uniform pulp, and a solvent is added to the pulp. The solvent may be, for example, water, ethanol, etc., and stirred so that the effective ingredients in the pulp are fully dissolved in the solvent. After filtering out the solids, it is concentrated by vacuum distillation to obtain concentrated boysenberry juice. In some other embodiments, microwave extraction, enzymatic extraction, etc. may also be used for extraction, which is not limited in this application. Boysenberry powder or concentrated boysenberry powder can be obtained by physically crushing boysenberries.

[0027] White onion is a powder made from the bulbs of the Allium genus of the Liliaceae family, which is processed by cutting, cleaning, pretreatment, crushing, juicing, sterilization, concentration, spraying, drying and other processes. White onion powder or extract also has the effects of lowering blood sugar, preventing cardiovascular diseases, and improving skin health. White onion extract can be extracted in the following way: select fresh, mature and disease-free white onions, peel and wash them, and then dry them. After drying, crush them with a grinder and pass them through a 60-80 mesh sieve to obtain a uniform powder. Mix and stir the white onion powder with an extractant (such as water or ethanol) in a ratio of (1:15-20, g / ml), reflux extraction at a temperature of 65-75°C, and the extraction time is generally 2-4 hours. During the extraction process, the raw materials need to be stirred intermittently 2-3 times every 0.5 hours, each time for 3-5 minutes, to promote the dissolution of the active ingredients. After multiple extractions, the solution system is combined and concentrated by vacuum distillation to obtain white onion powder or extract. In some other embodiments, microwave extraction, enzymatic extraction, etc. can also be used for extraction, which is not limited in the present application. White onion powder can be obtained by physically crushing white onions.

[0028] The applicant has found that the above-mentioned concentrated boysenberry juice or powder and white onion powder or extract are compounded in a ratio of 2-8.1:1, so that the formed composition or beverage has a good whitening effect. When the ratio of concentrated boysenberry juice or powder to white onion powder or extract exceeds the ratio range, although it still has a whitening effect, the whitening effect is not good. At the same time, the applicant has also found that when it is compounded with other ingredients, the whitening effect is reduced.

[0029] The boysenberry composition can be used alone for skin whitening, or as a component for skin whitening. The composition can also be used to prepare a skin whitening drug, or as a component for preparing a skin whitening drug.

[0030] As another aspect of the present application, the present application provides a boysenberry drink with whitening function, wherein the active ingredients of the boysenberry drink are composed of concentrated boysenberry juice or powder, white onion powder or extract, and water, wherein the weight ratio of concentrated boysenberry juice or powder to white onion powder or extract is 1-8.1:1, and the concentration of concentrated boysenberry juice is 200-356 μg / ml. The water may refer to drinking purified water or other drinkable water, such as distilled water. The drink with whitening function may also include the above-mentioned composition with whitening function and water. It should be noted that the active ingredients in the drink may include only concentrated boysenberry juice or powder, white onion powder or extract, and water.

[0031] The boysenberry drink can be used alone for skin whitening, or used as an ingredient for skin whitening. The drink can also be used to prepare a skin whitening drug, or used as an ingredient for preparing a skin whitening drug.

[0032] In addition, in order to improve the taste and / or color of the beverage, the beverage may also include additives that can be added to food, and the additives are selected from one or more of collagen peptides, sodium hyaluronate, fruit juice, fruit powder, sugar alcohol, steviol glycoside, and edible flavors. It should be noted that the amount of any additive added should be less than or equal to the maximum value allowed for addition.

[0033] The boysenberry drink can be prepared as follows:

[0034] The concentrated boysenberry juice or powder, white onion powder or extract and water are mixed evenly to obtain the drink, wherein the weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 1-8.1:1. In some embodiments, additives can also be added during the preparation process. Specifically, the additives can be added to part of the water in advance, heated and stirred until the colloid swells, and then allowed to stand to check whether the colloid is clear and uniform. After observing the state, if there are no particles and the state is uniform, the concentrated boysenberry juice, white onion powder or extract and part of the water are added to the system formed by mixing, and then the volume is fixed. In this way, the additives can be fully swollen before being mixed with other ingredients to avoid agglomeration and the like in the drink. The concentration of white onion powder or extract in the beverage can be, for example, 2-20 mg / ml, such as 2 mg / ml, 5 mg / ml, 8 mg / ml, 10 mg / ml, 11 mg / ml, 15 mg / ml, 18 mg / ml, 20 mg / ml, etc., or it can be 2-10 mg / ml, 10-20 mg / ml, 5-15 mg / ml, 2-15 mg / ml, 5-20 mg / ml, 2-5 mg / ml, etc.

[0035] After the entire system is filled with water to a fixed volume, the system can also be filtered to filter out some of the solids in the system, wherein a 80-mesh filter can be used, for example. In some embodiments, multiple filtrations can be performed until the solid content is less than or equal to 30±1%, the density is 1.08-1.12 g / ml, and the pH value is 3.9±0.2.

[0036] This embodiment does not limit this. Furthermore, the filtered system can be sterilized in various ways, such as high temperature sterilization, ultraviolet light sterilization, etc. In a more specific embodiment, during sterilization, the system can be filled first, and then placed in an environment of 105°C or above for at least 20 minutes for sterilization. After sterilization, it can be labeled and transferred to storage.

[0037] The boysenberry drink can be used alone for skin whitening or used to prepare products with skin whitening effect, or used as an ingredient for skin whitening or used to prepare products with skin whitening effect.

[0038] The present application is described in detail below in conjunction with specific embodiments.

[0039] Example 1

[0040] Weigh concentrated boysenberry juice and white onion powder and mix with water to form a drink. In the drink, the concentration of white onion powder is 2 mg / ml, the concentration of concentrated boysenberry juice is 2 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder is 1:1.

[0041] Example 2

[0042] Weigh concentrated boysenberry juice and white onion powder and mix with water to form a drink. In the drink, the concentration of white onion powder is 2.66 mg / ml, the concentration of concentrated boysenberry juice is 5.34 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder is about 2:1.

[0043] Example 3

[0044] Concentrated boysenberry juice and white onion powder were weighed and mixed with water to form a drink. In the drink, the concentration of white onion powder was 2.2 mg / ml, the concentration of concentrated boysenberry juice was 17.8 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder was about 8.1:1.

[0045] Example 4

[0046] Concentrated boysenberry powder and white onion extract were weighed and mixed with water to form a drink. In the drink, the concentration of white onion extract was 2.22 mg / ml, the concentration of boysenberry powder extract was 10 mg / ml, and the weight ratio of concentrated boysenberry powder to white onion extract was about 4.5:1.

[0047] Example 5

[0048] Weigh concentrated boysenberry powder and white onion extract and mix them with water to form a drink. In the drink, the concentration of white onion extract is 3 mg / ml, the concentration of concentrated boysenberry powder is 15 mg / ml, and the weight ratio of concentrated boysenberry powder to white onion extract is 5:1.

[0049] Example 6

[0050] Collagen peptide, sodium hyaluronate and edible flavor are added as additives to a portion of water, heated and stirred until the colloid is swollen and then allowed to stand, concentrated boysenberry powder, white onion extract and a portion of water are mixed evenly, and then mixed evenly with the additive solution system after standing to obtain a drink. In the drink, the concentration of white onion extract is 3 mg / ml, the concentration of concentrated boysenberry powder is 15 mg / ml, and the weight ratio of concentrated boysenberry powder to white onion extract is 5:1.

[0051] Example 7

[0052] Collagen peptide, sodium hyaluronate, fruit powder, fruit juice, sugar alcohol, edible flavor and the like are added as additives to part of the water, heated and stirred until the colloid is swollen and then allowed to stand, concentrated boysenberry juice and white onion powder are weighed and mixed with water, and then mixed evenly with the additive solution system after standing to obtain a drink, wherein the concentration of white onion powder in the drink is 2 mg / ml, the concentration of concentrated boysenberry juice is 2 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder is 1:1.

[0053] Example 8

[0054] Collagen peptide, sodium hyaluronate, fruit powder, fruit juice, sugar alcohol, and edible flavors are added as additives to part of the water, heated and stirred until the colloid is swollen and then allowed to stand, concentrated boysenberry juice and white onion powder are weighed and mixed with water, and then mixed evenly with the additive solution system after standing to obtain a drink, wherein the concentration of white onion powder in the drink is 2.66 mg / ml, the concentration of concentrated boysenberry juice is 5.34 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder is 2:1.

[0055] Example 9

[0056] Collagen peptides, sodium hyaluronate, fruit powder, fruit juice, sugar alcohol, edible flavors and the like are added as additives to part of the water, heated and stirred until the colloid is swollen and then allowed to stand, concentrated boysenberry juice and white onion powder are weighed and mixed with water, and then mixed evenly with the additive solution system after standing to obtain a drink, wherein the concentration of white onion powder in the drink is 2.2 mg / ml, the concentration of concentrated boysenberry juice is 17.8 mg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder is about 8.1:1.

[0057] Comparative Example 1

[0058] Arbutin was weighed and mixed with water to form a drink. The concentration of arbutin in the drink was 3000 μg / ml.

[0059] Comparative Example 2

[0060] The white onion extract was weighed and mixed with water to form a drink. The concentration of the white onion extract in the drink was 500 μg / ml.

[0061] Comparative Example 3

[0062] Weigh the concentrated boysenberry powder and mix it with water to form a drink. The concentration of the concentrated boysenberry powder in the drink is 800 μg / ml.

[0063] Comparative Example 4

[0064] Concentrated boysenberry juice and white onion powder were weighed and mixed with water to form a drink. In the drink, the concentration of white onion powder was 267 μg / ml, the concentration of concentrated boysenberry juice was 133 μg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder was about 1:2.

[0065] Comparative Example 5

[0066] Concentrated boysenberry powder and white onion powder were weighed and mixed with water to form a drink. In the drink, the concentration of white onion powder was 24 μg / ml, the concentration of concentrated boysenberry juice was 376 μg / ml, and the weight ratio of concentrated boysenberry juice to white onion powder was about 15.7:1.

[0067] Comparative Example 6

[0068] Weigh concentrated boysenberry juice, white onion powder, white tomato powder, emblica powder, concentrated pomegranate powder, olive fruit powder and red grape yeast compound powder, mix with water to form a drink after constant volume, wherein the weight ratio of concentrated pomegranate powder to the whole drink is 3wt%, the weight ratio of red grape yeast compound powder to the whole drink is 0.45wt%, the weight ratio of olive fruit powder to the whole drink is 0.05wt%, the weight ratio of emblica powder to the whole drink is 0.3wt%, the weight ratio of white tomato powder to the whole drink is 0.8wt%, the weight ratio of concentrated boysenberry juice to the whole drink is 1.6wt%, and the weight ratio of white onion powder to the whole drink is 0.8wt%. The weight ratio of concentrated boysenberry juice to white onion powder is 2:1.

[0069] The drink obtained above was diluted to a concentration of 267 μg / ml of concentrated boysenberry juice and 133 μg / ml of white onion powder, and then set aside.

[0070] That is, the final drink, compared with Example 2, has a certain amount of white tomato powder, emblica powder, concentrated red pomegranate powder, olive fruit powder and red grape yeast composite powder added.

[0071] Comparative Example 7

[0072] The concentrated boysenberry juice was weighed and mixed with water to form a drink, wherein the concentration of the concentrated boysenberry juice in the drink was 1200 μg / ml.

[0073] Experimental Example 1

[0074] The whitening efficacy of the boysenberry beverage obtained in the embodiment was tested, and the testing process was as follows:

[0075] 1. Test materials

[0076] 1.1. Sample preparation information

[0077] Experimental Group:

[0078] Example 1-3: Example 1-3 was diluted, specifically:

[0079] 1.1.1 The drink obtained in Example 1 was diluted 10 times to obtain a drink having a concentration of 200 μg / ml of white onion powder and a concentration of 200 μg / ml of concentrated boysenberry juice;

[0080] 1.1.2 The drink obtained in Example 2 was diluted 20 times to obtain a drink having a concentration of 133 μg / ml of white onion powder and a concentration of 267 μg / ml of concentrated boysenberry juice;

[0081] 1.1.3 The drink obtained in Example 3 was diluted 50 times to obtain a drink having a concentration of 44 μg / ml of white onion powder and a concentration of 356 μg / ml of concentrated boysenberry juice;

[0082] 1.1.4 Beverages obtained in Comparative Examples 1-6.

[0083] Control group: Arbutin, white powder, batch number C2219112, Shanghai Aladdin Biochemical Technology Co., Ltd., refrigerated storage. The solvent is standard dilution water.

[0084] 1.2. Experimental animals

[0085] Zebrafish were kept in aquaculture water at 28°C (water quality: 200 mg instant sea salt was added to 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO 3), bred and provided by our company's fish farming center, the experimental animal use license number is: SYXK(Zhejiang)2022-0004, the breeding and management complies with the requirements of the international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2024-8764-01.

[0086] 1.3. Instruments, consumables and reagents

[0087] Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd., China); precision electronic balance (CP214, OHAUS, USA); 6-well plate (Zhejiang Bellanbo Biotechnology Co., Ltd., China).

[0088] Methylcellulose (Batch No. C2004046, Shanghai Aladdin Biochemical Technology Co., Ltd., China).

[0089] 2. Detection Method

[0090] 2.1. Detection principle

[0091] The structure and function of zebrafish skin are similar to those of humans. The melanin production pathway is conserved with that of humans. The genes related to the melanin production process (such as tyr, mitf, sox10 and dct) are homologous to humans. When the zebrafish embryo develops to 24 hours, melanin begins to grow from the retinal epithelium. Pigment cells (including melanocytes, flavin cells and iridophores) originate from a group of cells differentiated from the dorsal ectoderm - neural crest cells, and then proliferate, migrate and differentiate into pigment mother cells. Among them, melanocytes synthesize melanin, which is similar to the melanocytes of vertebrates. Intervention in the process of melanin formation can inhibit the formation of melanin, mainly by inhibiting the activity of tyrosinase, thereby reducing the production of melanin. Zebrafish embryos are transparent throughout their bodies in the early stages of development. After sample processing, the melanin formation process of zebrafish can be directly observed under a microscope. The whitening effect of the sample is evaluated by analyzing the changes in the intensity of the melanin signal on the zebrafish head. The lower the intensity of the melanin signal on the zebrafish head, the higher the inhibition rate of melanin and the stronger the whitening effect.

[0092] 2.2. Evaluation of whitening efficacy

[0093] 6hpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The samples were given water-soluble, and the positive control arbutin was given a sample dose of 3000μg / mL. At the same time, a normal control group was set up, and the capacity of each well was 3mL. Examples 1-3 (the diluted beverage in 1.1) and comparative examples 4-6 were given a sample dose of 400μg / mL, comparative example 1 was given a sample dose of 3000μg / mL, comparative example 2 was given a sample dose of 800μg / mL, and comparative example 3 was given a sample dose of 500μg / mL. After 2 days of treatment at 28℃, 10 zebrafish were randomly selected from each experimental group and placed under a dissecting microscope to take pictures and save the pictures. NIS-Elements D 3.20 advanced image processing software analyzed and collected data, analyzed the melanin signal intensity (mean±SE) of the zebrafish head, and the statistical analysis results of this indicator were used to evaluate the whitening efficacy of the samples. The whitening efficacy formula is as follows:

[0094]

[0095] The statistical results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis, and p < 0.05 indicated that the difference was statistically significant.

[0096] 3. Test results

[0097] Whitening effect

[0098] Table 1. Sample whitening efficacy test results (n=10)

[0099]

[0100] Compared with the normal control group, **p<0.01, ***p<0.001

[0101] See also Figure 1-Figure 3 ,in Figure 1 A typical graph of melanin signal intensity in the zebrafish head after sample processing is shown, where the framed area is the analysis area. Figure 2 A data graph showing the melanin signal intensity of the zebrafish head after sample treatment and the melanin signal intensity of the zebrafish head in the blank control group. Figure 3 The whitening efficacy data of each sample is shown.

[0102] In this experimental example, in order to adapt to the physiological habits of zebrafish, the boysenberry beverage used in Examples 1-3 was diluted a certain multiple. It can be understood that the experimental results verified by the experimental data obtained from the experiment on the diluted beverage are also applicable to the beverage before dilution.

[0103] From Table 1 and Figure 1-Figure 3It can be seen that compared with the blank control group, Comparative Examples 1-5 and Examples 1-3 all have whitening effects, among which Comparative Examples 2-5 have lower whitening effects than Comparative Example 1. At the same time, the whitening effects of the beverages of Examples 1-3 are significantly higher than those of Comparative Example 1. It can be seen that by compounding white onion powder or extract and concentrated boysenberry juice or powder in an appropriate ratio, the whitening effect can be significantly improved. Compared with Comparative Examples 2 and 3, in Examples 1-3, when the concentration of the active ingredient (400 μg / mL) is less than that of Comparative Example 2 (800 μg / mL) and Comparative Example 3 (500 μg / mL), the whitening effect is significantly improved by about 20%. It can be seen that by making a reasonable choice of the ratio of concentrated boysenberry juice or powder and white onion powder or extract, the whitening effect of the beverage can be significantly improved.

[0104] The difference between Examples 1-3 and Comparative Examples 4 and 5 is only that the ratio of white onion powder or extract to concentrated boysenberry juice or powder is different. By comparing Examples 1-3 and Comparative Examples 4 and 5, it can be seen that in Examples 1-3, when the concentration of the active ingredient (400 μg / mL) is the same, the ratio of concentrated boysenberry juice or powder to white onion powder or extract is selected in the present application to be in the range of 1-8.1:1. Under the ratio of white onion powder or extract to concentrated boysenberry juice or powder in this range, the whitening effect can be increased to more than 50%, an increase of about 20%, which significantly improves the whitening effect of the product.

[0105] The difference between Examples 1-3 and Comparative Example 6 is that, at the same concentration of concentrated boysenberry juice or powder and white onion powder or extract after dilution (400 μg / mL), the whitening effect of the beverages obtained in Examples 1-3 of the present application reaches more than 50%, compared with the beverage containing 7 active ingredients in Comparative Example 6, the maximum improvement in whitening effect reaches 20%, indicating that the beverage comprising only two components, white onion powder or extract and concentrated boysenberry juice or powder, has better whitening effect, indicating that beverages with more components may instead lead to a decrease in whitening effect.

[0106] Experimental Example 2

[0107] The drinks obtained in Comparative Examples 1, 2 and 7 were subjected to an anti-glycation test, and the test process was as follows:

[0108] 1. Test materials

[0109] 1.1. Sample preparation information

[0110] Experimental group: the drinks obtained from comparative examples 1, 2 and 7, the drinks contained water and the composition with whitening function, and the solvent was 0.4M glucose solution.

[0111] Control group: blank control. The solvent is 0.4M glucose solution.

[0112] 1.2. Experimental animals

[0113] Zebrafish were kept in aquaculture water at 28°C (water quality: 200 mg instant sea salt was added to 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO 3 ), bred and provided by our company's fish farming center, the experimental animal use license number is: SYXK(Zhejiang)2022-0004, the breeding and management complies with the requirements of the international AAALAC certification (certification number: 001458), IACUC ethics review number: IACUC-2024-8764-01.

[0114] 1.3. Instruments, consumables and reagents

[0115] Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd., China); precision electronic balance (CP214, OHAUS, USA); 6-well plate (Zhejiang Bellanbo Biotechnology Co., Ltd., China); multifunctional microplate reader (SPARK, TECAN, Austria); high-speed refrigerated centrifuge (Heraeus Fresco17, ThermoFisher, Germany); fully automatic sample rapid grinding instrument (JXFSTPRP-24L, Shanghai Jingxin Experimental Equipment Technology Department, China); constant temperature oscillator (HZ-9211K, Taicang Hualida Experimental Equipment Co., Ltd., China); black 96-well plate (Nest Biotech, China).

[0116] D-(+) glucose (lot number A2425167, Shanghai Aladdin Biochemical Technology Co., Ltd., China); PBS buffer (lot number BL601A, Biosharp, China).

[0117] 2. Detection Method

[0118] 2.1. Detection principle

[0119] Glycation refers to the non-enzymatic reaction between proteins and reducing sugars such as glucose and fructose. This reaction can change the structure and function of proteins and generate glycation end products (AGEs). The accumulation of AGEs in the body can lead to diabetes, Alzheimer's disease, and cardiovascular diseases. Effective and accurate detection of AGE levels can help evaluate physiological and pathological changes in the body. AGEs are divided into the following three types: (i) fluorescent cross-linked AGEs, such as pentosidine; (ii) non-cross-linked AGEs, such as carboxymethyl lysine (CML); (iii) non-fluorescent cross-linked AGEs, such as imidazole dilysine. Feeding zebrafish with glucose to establish a high-sugar model is to cause a glycation reaction in the zebrafish, resulting in a large accumulation of AGEs. The fluorescence value of fluorescent cross-linked AGEs is detected under 370nm excitation light and 440nm absorption light, and this indicator is used to analyze and evaluate the anti-glycation efficacy of the sample. The lower the AGEs fluorescence value, the stronger the anti-glycation efficacy.

[0120] 2.2. Evaluation of anti-glycation efficacy

[0121] 4dpf wild-type AB strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The samples were given water-soluble and a blank control group was set up, with a capacity of 3mL per well. After 1 day of treatment at 28°C, the zebrafish in each experimental group were washed 3 times with standard diluted water and transferred to a 1.5mL centrifuge tube. 10 zebrafish were treated in each tube (experimental group), and the capacity of each tube was 150μL. Except for the normal control group, the other experimental groups were given a working solution prepared with 0.4M glucose solution to establish a zebrafish AGEs increase model. Three parallel experiments were set up for each experimental group. After 1 day of treatment on a 60°C shaker, each experimental group was ground and centrifuged to obtain the supernatant, and a multifunctional microplate reader was used to collect data, and the AGEs fluorescence value (S) of each experimental group was analyzed. The anti-glycation efficacy of the sample was evaluated based on the statistical analysis results of this indicator. The anti-glycation efficacy formula is as follows:

[0122]

[0123] The statistical results were expressed as mean ± SE. SPSS 26.0 software was used for statistical analysis, and p < 0.05 indicated that the difference was statistically significant.

[0124] 3. Test results

[0125] Anti-glycation effect

[0126] Under the experimental conditions, the fluorescence value of zebrafish AGEs in the experimental group at a concentration of 500-2000 μg / mL was significantly weakened compared with the model control group, indicating that it has anti-glycation effect, and the anti-glycation effect can reach up to 7%. See Table 2 for details, where *p<0.05, **p<0.01, ***p<0.001.

[0127] Table 2. Results of the anti-glycation efficacy test of samples (n=3)

[0128]

[0129] Compared with the model control group, *p<0.05, **p<0.01, ***p<0.001

[0130] Glycation is a spontaneous physiological process that is closely related to aging. Advanced glycation end products (AGEs) are brown substances formed in the late stage of the glycosylation reaction between glucose or other reducing sugars and free amino groups in proteins, nucleic acids or lipids. After being produced in the human body, AGES will continue to accumulate, regulating gene expression, destroying protein structure, mediating a series of signal pathways by binding to RAGES, affecting cell apoptosis and differentiation, thereby causing damage to human health and accelerating aging. For human skin, as the content of AGE in the skin increases, the skin will show symptoms such as yellowing, browning, poor elasticity, deepening wrinkles, and dark yellow skin.

[0131] By inhibiting and reducing the generation and accumulation of AGEs or removing AGES in the human body (i.e., anti-glycation), the damage of AGES to the skin can be reduced, thereby playing a certain whitening effect. Compared with the blank control group, both Comparative Example 2 and Comparative Example 7 have a certain anti-glycation effect. Comparative Example 2 contains only white onion powder or extract ingredients, and Comparative Example 7 contains only concentrated boysenberry juice or powder ingredients. It can be seen from the data in Table 7 that concentrated boysenberry juice or powder and white onion powder or extract can both play an anti-glycation role. In this embodiment, when concentrated boysenberry juice or powder and white onion powder or extract are used in combination, they can also play a certain anti-glycation role on the human body, thereby improving the whitening effect on the skin.

[0132] Experimental Example 3

[0133] The boysenberry drinks obtained in Examples 7-9 were subjected to sensory, microbiological, and physical and chemical tests. The test methods and results are shown in Tables 3 to 7:

[0134] Table 3 Sensory test results

[0135]

[0136] From the results in Table 3, it can be seen that the sensory tests of the boysenberry drinks obtained in Examples 7-9 meet the requirements of relevant national standards.

[0137] Table 4 Microbiological test results

[0138] Test items unit Detection Methods Test results Limits illustrate Mold CFU / mL GB4789.15-2.16 Method 1 ＜1 ≤20 conform to yeast CFU / mL GB4789.15-2.16 Method 1 ＜1 ≤20 conform to

[0139] From the results in Table 4, it can be seen that the microbial detection of the boysenberry beverages obtained in Examples 7-9 meets the requirements of relevant national standards.

[0140] Table 5 Colony Detection Results

[0141]

[0142] As can be seen from the results in Table 5, the colony detection of the boysenberry drinks obtained in Examples 7-9 meets the relevant national standard requirements.

[0143] Table 6 Physicochemical Detection Results

[0144] Test items unit Detection Methods Test results Limit of Quantitation Limits illustrate lead mg / Kg GB5009.12-2017 Second Law ND 0.05 ≤0.3 conform to

[0145] Note: ND = Not Detected

[0146] As can be seen from the results in Table 6, the physicochemical detection of the boysenberry drinks obtained in Examples 7-9 meets the relevant national standard requirements.

[0147] Experimental Example 4

[0148] An acute oral toxicity test was conducted on the boysenberry drinks obtained in Examples 7-9. The test method referred to the "National Food Safety Standard Acute Oral Toxicity Test" GB 15193.3-2014.

[0149] 1. The test process is as follows:

[0150] 1.1 Detection environment: Barrier environment animal house, experimental animal use license number: SYXK(Yue)2023-0086; room temperature 22±2°C, humidity 62±5%.

[0151] 1.2 Experimental animals and feed: 20 SPF-grade Kunming mice (10 females and 10 males), body weight 18.0g - 20.0g; experimental animals and feed were purchased from the Guangdong Provincial Medical Experimental Animal Center. Experimental animal production license number: SCXK(Yue)2022-0002; animal certificate number: No.44007200129050.

[0152] 1.3 Sample treatment: Weigh 50.0g of the drink sample and mix it evenly with ultrapure water to 100mL to obtain the test solution.

[0153] 1.4 Test steps:

[0154] 1.4.1 Dose: The limit method was used in the formal test, and the dose of the test substance was set at 10000mg / kg body weight;

[0155] 1.4.2 Administration: Administer the test solution orally once by gavage needle at a dose of 0.2mL / 10g body weight. The animals were fasted for 4h before gavage and allowed free access to water. Normal diet was given 1h after gavage;

[0156] 1.4.3 Observation: The experiment was observed for 14 days, and the poisoning symptoms and deaths were recorded. During the observation period, the living animals were weighed once a week. At the end of the observation period, the surviving animals were humanely killed and autopsied.

[0157] 2. Test results

[0158] During the observation period, no obvious poisoning symptoms or death were observed in all the test animals, and no abnormalities were found in the gross anatomy of the experimental animals. 50 >10000mg / kg body weight.

[0159] Table 7 Results of acute oral toxicity test of beverages on mice

[0160]

[0161] From the data in Table 7, we can see that the acute oral toxicity LD of boysenberry beverage is 50 >10000mg / kg body weight, refer to the acute toxicity (LD 50 ) dosage classification table, which is a practically non-toxic substance.

[0162] Experimental Example 5

[0163] The effects of the boysenberry drinks obtained in Examples 7-9 were evaluated. The drinks contained water and a composition with whitening function:

[0164] Evaluation items: Under normal conditions, adult subjects consumed the product continuously for 28 days according to the instructions, and the changes in the subjects' skin color and skin yellowing parameters were evaluated.

[0165] Subjects: A total of 31 valid subjects completed the assessment, including healthy Chinese women, aged 31 to 54 years old, with an average age of 43.74±6.87, who met the voluntary inclusion and exclusion criteria.

[0166] Assessment Area: Oral

[0167] Directions: Drink directly. Take 1 bottle before lunch and 1 bottle before dinner.

[0168] Use period: 28 days

[0169] Evaluation period: before consuming the product (D0). 28 days after consuming the product (D28)

[0170] Evaluation parameters:

[0171] 1.VISIA is used to collect facial images, and IPP analyzes the skin color of the collected photos

[0172] 2. Expert evaluation

[0173] 3. Subject Self-Assessment

[0174] Before the evaluation begins, laboratory technicians screen eligible subjects who meet the evaluation requirements according to the evaluation requirements and record the Fitzpatrick type and skin type (dry, mixed to dry, normal, oily or mixed to oily skin) of the subjects' faces.

[0175] Starting from D0, the subjects cleansed their faces with a facial cleanser and dried their skin with a dandruff-free dry facial tissue, and sat in a laboratory at a temperature of 21±2℃ and 50±10% RH for 30 minutes. They were evaluated by experts and those who met the inclusion criteria entered the next test. Facial VISIA and inner arm AGEreader measurements were collected from qualified subjects. The subjects filled out a consumer use test questionnaire before use. The test products were distributed to the subjects, and the subjects were instructed to consume them according to the product usage requirements, and written test precautions and product usage logs were provided.

[0176] D28

[0177] The subjects cleansed their face with a facial cleanser and wiped their skin with a dandruff-free facial tissue. They sat in a laboratory at 21±2℃ and 50±10%RH for 30 minutes. Experts evaluated the facial condition of the subjects. VISIA values ​​of the subjects' faces and AGEreader values ​​of the inner arms were collected. The subjects filled in the consumer use test questionnaire and self-assessment questionnaire. The product use logs were checked and collected.

[0178] Statistics:

[0179] SPSS 28.0 was used to analyze the data. The normal distribution test was performed on the evaluation data. If the evaluation data was normally distributed, the paired t test method was used for statistical analysis; if the evaluation data was non-normally distributed, the rank sum test method was used for statistical analysis. The rank sum test was used for statistical analysis. The statistical method significance level was p < 0.05.

[0180] The statistical results are shown in Tables 8 to 11:

[0181] Table 8 Machine evaluation test results

[0182]

[0183] Among them, the measurement basis of each evaluation parameter in Table 8 is as follows:

[0184] VISIA is used to collect facial images, and IPP performs color analysis on the collected photos. If the skin color L* analysis value increases, the skin color becomes brighter; if the skin color a* analysis value decreases, the skin redness decreases; if the skin color b* value analysis value decreases, the skin yellowness decreases; if the skin color ITA° value analysis value increases, the skin color becomes lighter.

[0185] It can be seen from the data in Table 8 that after consuming the boysenberry drink provided in this experimental example, the skin condition of each subject was effectively improved in all dimensions.

[0186] Table 9 Expert evaluation test results

[0187]

[0188] From the data in Table 9, it can be seen that after consuming the product for 28 days, the mean yellowing value assessed by dermatologists was significantly reduced by 0.30.

[0189] Table 10 Test results of self-assessment (skin condition) by subjects

[0190]

[0191] From the data in Table 10, it can be seen that after consuming the product for 28 days, the mean self-rating of the subjects for improvement in facial dullness was significantly reduced by 2.45, and the mean self-rating of the subjects for yellowing of facial skin was significantly reduced by 2.32.

[0192] Table 11 Test results of self-assessment (satisfaction) of subjects

[0193]

[0194] It can be seen from the data in Tables 8 to 11 that after consuming the boysenberry drink provided in this experimental example, the skin color of the subjects was significantly improved in all dimensions.

[0195] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A boysenberry composition with whitening function, characterized in that: The active ingredients in the composition are composed of concentrated boysenberry juice or powder and white onion powder or extract, and the weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 1-8.1:

1.

2. The boysenberry composition with whitening function according to claim 1, characterized in that: The weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 2-8.1:

1.

3. A boysenberry drink with whitening function, characterized in that: The active ingredients in the drink are composed of concentrated boysenberry juice or powder, white onion powder or extract and water, wherein the weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 1-8.1:

1.

4. The boysenberry drink with whitening function according to claim 3, characterized in that: The weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 2-8.1:

1.

5. The boysenberry drink with whitening function according to claim 3, characterized in that: The concentration of the white onion powder or extract is 2-20 mg / ml.

6. The boysenberry beverage with whitening function according to any one of claims 3 to 5, characterized in that: The beverage also includes additives that can be added to food, and the additives are selected from one or more of collagen peptides, sodium hyaluronate, fruit juice, fruit powder, sugar alcohol, steviol glycoside, and edible flavors.

7. A method for preparing a boysenberry drink with whitening function, characterized in that: The concentrated boysenberry juice or powder, white onion powder or extract and water are uniformly mixed to obtain the product, wherein the weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 1-8.1:

1.

8. The method for preparing the boysenberry drink with whitening function according to claim 7, characterized in that: The weight ratio of the concentrated boysenberry juice or powder to the white onion powder or extract is 2-8.1:

1.

9. The method for preparing the boysenberry drink with whitening function according to claim 7, characterized in that: The concentration of the white onion powder or extract is 2-20 mg / ml.

10. The method for preparing a boysenberry drink with whitening function according to any one of claims 7 to 9, characterized in that: The method comprises: adding an additive to a portion of water, heating and stirring until the colloid is swollen and then standing, uniformly mixing the concentrated boysenberry juice or powder, the white onion powder or extract and a portion of water, and then uniformly mixing with the additive solution system after standing to obtain the product; Wherein, the additive is selected from one or more of collagen peptides, sodium hyaluronate, fruit juice, fruit powder, sugar alcohol, steviol glycoside, and edible flavors.

11. Use of the boysenberry composition according to claim 1 or 2 in preparing a skin whitening drug.

12. Use of the boysenberry beverage according to any one of claims 3 to 6 in the preparation of a skin whitening drug.

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