Procyanidine enhanced hair growth formula and wash supplies thereof

Abstract

The invention discloses a procyanidine enhanced hair growth formula and a wash supply thereof. The procyanidine enhanced hair growth formula comprises the following raw materials in percentage by mass: 30-40% of apple extract, 18-20% of glossy privet fruit extract, 12-14% of trifolium pretense flower extract, 12-14% of cacumen biotae extract and 12-14% of caffeine. 6.45%-6.95% of adenosine and 3.05%-3.35% of a ginseng fruit extract; in the apple extract, the content of procyanidine B2 is not less than 20%, the content of procyanidine C1 is not less than 15%, and the content of epicatechin is not less than 30%; according to the present invention, the plant extract is adopted as the main material, the juice is obtained through juicing, the preparation is convenient, the proanthocyanidins B2 and C1 in the apple extract and the epicatechin are adopted as the main active components, the source is wide, the production cost is low, the anti-hair loss effect is good, the irritation to the human skin is not generated, and the effect is mild.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, in particular to a proanthocyanidin-enhanced hair growth formula and a washing product thereof. Background Art

[0002] According to a survey conducted by the National Health Commission, 250 million people in my country will have hair loss problems in 2022, and those born in the 1990s are gradually replacing those born in the 1980s as the group with a high incidence of hair loss.

[0003] Hair loss is affected by many factors, including genetic factors, hormonal changes (such as hair loss caused by decreased estrogen levels after childbirth), medical conditions (such as surgery, chemotherapy, major diseases, etc.), nutritional deficiencies (such as the lack of vitamins, amino acids and trace elements, etc.), and aging. According to the causes of hair loss, it can be divided into physiological hair loss (such as normal hair loss and postpartum hair loss), telogen effluvium (caused by damage to the immune system due to long-term fatigue, staying up late or mental stress) and pathological hair loss (such as alopecia areata, male pattern baldness and fungal infections, etc.). In addition, hair loss can also be divided into transient hair loss and permanent hair loss according to the duration.

[0004] Different treatment methods are used for different types of hair loss. There are many ways to treat hair loss, mainly including chemotherapy, red light therapy and surgical treatment. Commonly used drugs in clinical practice include minoxidil, finasteride, dutasteride and spironolactone. Among them, finasteride and dutasteride are limited to men, while spironolactone is mainly used for the treatment of female male pattern baldness. These drugs are effective in the treatment of male pattern baldness, but they need to be taken for a long time and the side effects cannot be ignored. For example, long-term use of minoxidil may cause abnormal heart rate, hirsutism, etc., while the use of finasteride and dutasteride may cause male dysfunction and affect sperm activity. Compared with the use of red light therapy, it seems to be relatively safe, which can stimulate the production of collagen and improve the hair loss problem caused by excessive cortical secretion caused by excessive androgen. However, for male pattern baldness, the effect is not obvious. Surgical treatment is to transplant healthy hair follicles from the occipital region to the part of the hair loss through surgery to make it grow new hair. However, the surgical method is expensive and difficult to care for.

[0005] In addition to the above methods, natural plant extracts such as Chinese medicine are also widely used in the treatment of hair loss. For example, patent CN101810554A protects a hair growth preparation composed of plant extracts such as ginseng, salvia miltiorrhiza, Panax notoginseng, Dictamni, Chuanxiong and Zanthoxylum bungeanum; patent CN1013503B protects an anti-hair loss and hair growth preparation made from Chinese medicines such as Polygonum multiflorum, Angelica sinensis, Rehmannia glutinosa, Radix Rehmanniae, and Cuscuta chinensis; patent CN102885994A uses extracts made from Polygonum multiflorum, Platycladus orientalis, ginger and other Chinese medicines to make shampoo for protecting the scalp and preventing hair loss; patent CN101711731A uses alcohol to soak Chinese herbs such as Polygonum multiflorum, mulberry leaves, and safflower to prepare a transdermal smear to reduce hair loss and promote hair growth.

[0006] The above-mentioned several public technical solutions have their own characteristics, but all have some limitations: first, some ointments need to be washed after use, and the Chinese medicine smell is very strong, which is not suitable for the current fast-paced urban life; second, the preparation methods are mostly to obtain active ingredients through water boiling or alcohol extraction, resulting in a large loss of effective ingredients; third, Chinese medicine extracts often need to be boiled or extracted with alcohol during the preparation process, which will further lose effective ingredients, thereby reducing the effect of preventing hair loss and promoting hair growth. Therefore, a new formula for preventing hair loss and promoting hair growth is still needed to better solve the problem of hair loss among contemporary young people. Summary of the invention

[0007] In order to solve the defects of the prior art, the present invention provides a proanthocyanidin enhanced hair growth formula and a hair care product thereof.

[0008] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0009] The present invention discloses a proanthocyanidin enhanced hair growth formula, which comprises the following raw materials in terms of mass fraction: 30-40% apple extract, 18-20% privet fruit extract, 12-14% red clover flower extract, 12-14% orientalis leaf extract, and 12-14% caffeine;

[0010] Adenosine 6.45-6.95%, ginseng fruit extract 3.05-3.35%; the content of proanthocyanidin B2 in the apple extract is not less than 20%, the content of proanthocyanidin C1 is not less than 15%, and the content of epicatechin is not less than 30%.

[0011] As a preferred technical solution of the present invention, other active substances for treating and preventing hair loss are also included. The other active substances for treating and preventing hair loss include biotin skin conditioner, three-in-one anti-allergic agent and alcohol moisturizer.

[0012] As a preferred technical solution of the present invention, the content of the apple extract is 35%, the content of the Ligustrum lucidum fruit extract is 19.35%, the content of the red clover flower extract is 12.95%, the content of the Platycladus orientalis leaf extract is 12.90%, the content of the caffeine is 13.45%, the content of the adenosine is 6.55%, and the content of the ginseng fruit extract is 3.05%.

[0013] As a preferred technical solution of the present invention, a washing product comprises a hair growth composition made from the above-mentioned proanthocyanidin-enhanced hair growth formula.

[0014] As a preferred technical solution of the present invention, the content of the hair growth composition in the toiletries is 0.1-10 wt %.

[0015] As a preferred technical solution of the present invention, the content of the hair growth composition in the toiletries is 5-10 wt %.

[0016] The beneficial effects of the present invention are:

[0017] 1. The apple extract in this proanthocyanidin-enhanced hair growth formula is rich in proanthocyanidin B2 polyphenol compounds, such as proanthocyanidin monomer epicatechin, proanthocyanidin B2, and proanthocyanidin C1.

[0018] Among them, proanthocyanidin B2 has a good antioxidant effect, reducing the damage of free radicals to the scalp. As a selective protein kinase C (PKC) inhibitor, it prevents hair follicle cells from entering the catagen or telogen phase prematurely, keeping the hair follicles in the growth phase longer. Therefore, proanthocyanidin B2 can promote the proliferation and differentiation of hair follicle cells, help the growth of hair epithelial cells and stimulate the induction of growth hormone. Among them, proanthocyanidin B2 is an inhibitor of inflammasome activation. It can reduce the cytoplasmic pool of NLRP3 and pro-IL-1, inhibit the assembly of inflammasomes, the subsequent activation of cysteinyl enzyme-1 and IL-1, and thus reduce the level of extracellular IL-1 secreted by macrophages in humans stimulated by LPS.

[0019] 2. The apple extract in this proanthocyanidin-enhanced hair growth formula is rich in proanthocyanidin C1. As a natural compound, proanthocyanidin C1 has significant hair growth activity. Proanthocyanidin C1 has shown the effect of promoting the proliferation of hair follicle cells in in vitro experiments. It can prolong the growth period of hair follicles by activating the Wnt / β-catenin signaling pathway, thereby promoting the proliferation of hair follicle cells and hair regeneration. In addition, the hair growth activity of proanthocyanidin C1 is also related to its anti-aging and anti-inflammatory properties. It can reduce the inflammatory response around the hair follicles, improve the microenvironment of the hair follicles, and promote hair regeneration.

[0020] 4. The Ligustrum lucidum extract in this proanthocyanidin-enhanced hair growth formula is rich in oleanolic acid, which can promote the expression of β-catenin and VEGF in hair follicles and inhibit TGF-β1. It can promote the proliferation of hair follicle matrix cells, increase the thickness of the dermis, and promote the growth of hair shafts. In addition, it can inhibit the activity of 5-α reductase and reduce the production of DHT. While promoting hair growth, it can also reduce hair loss.

[0021] 5. The Platycladus orientalis leaf extract in this proanthocyanidin-enhanced hair growth formula is rich in plant polyphenols, has good antibacterial and anti-inflammatory effects, and helps maintain the health of the scalp barrier. The Platycladus orientalis leaf extract can inhibit 5-α reductase, reduce the synthesis of DHT, activate tyrosinase to promote the synthesis of hair melanin, activate the Wnt / β-catenin pathway to activate hair follicle stem cells to promote the development of new hair follicles, and promote the expression of growth factors such as IGF-1 and VEG, promoting the proliferation of hair follicle cells and the formation of surrounding capillaries.

[0022] 6. The caffeine in this proanthocyanidin-enhanced hair growth formula can reduce the inhibitory effect of dihydrotestosterone (DHT) on hair follicle growth, promote the proliferation and differentiation of dermal papilla cells and the expression of growth factors such as IGF-1 and KGF, and activate potassium channels to promote blood circulation in the scalp. Ginseng extract is rich in ginsenosides, which can inhibit 5-α reductase to reduce the production of DHT, inhibit the TGF-β pathway and reduce cell apoptosis. At the same time, ginsenosides can activate signaling pathways such as Wnt / β-catenin, Shh / Gli and ERK, promote the expression of growth factors such as VEGF and EGF, and promote the proliferation and survival of hair follicle cells. Adenosine is an inherent substance in human cells and has the function of promoting blood circulation. In addition, adenosine can promote the production of FGF7, stimulate hair growth and produce thicker hair. It can directly act on dermal papilla cells and play an important role in the growth of existing and new hair. Biotin, also known as vitamin B7, can improve the keratin structure of hair and prevent hair from becoming dry and brittle. While nourishing the scalp, it also strengthens the hair and adds volume and shine.

[0023] 7. This proanthocyanidin-enhanced hair growth formula is mainly based on plant extracts, which are obtained directly by squeezing juice, and is easy to manufacture. It uses apple extract (proanthocyanidins B2, C1 and epicatechin) as the main active ingredient, has a wide source, and has a low production cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The results of tissue control tests on the extraction of proanthocyanidins from the apple extract of the present invention are shown;

[0025] Figure 2The graph shows the growth-promoting activity of the apple extracts of different concentrations on mouse hair epithelial cells;

[0026] Figure 3 The animal data proof effect diagram of the present invention is shown;

[0027] Figure 4 This is a graph showing the effect of effectively improving hair density after using the hair growth essence provided by the present invention for 2 months.

[0028] Figure 5 This is a graph showing that hair density is significantly improved after subjects used the hair growth essence provided by the present invention for 4 months.

[0029] Figure 6 This is a graph showing the significant improvement in hair density after the subjects used the hair growth essence provided by the present invention for 6 months. DETAILED DESCRIPTION

[0030] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0031] Preparation Example 1: A proanthocyanidin-enhanced hair growth formula of the present invention, which comprises the following raw materials by mass fraction: 30-40% apple extract, 18-20% privet fruit extract, 12-14% red clover flower extract, 12-14% orientalis leaf extract, and 12-14% caffeine;

[0032] Adenosine 6.45-6.95%, ginseng fruit extract 3.05-3.35%; the content of proanthocyanidin B2 in the apple extract is not less than 25%, the content of proanthocyanidin C1 is not less than 10%, and the content of epicatechin is not less than 30%.

[0033] The invention also includes other active substances for treating and preventing hair loss, including biotin skin conditioner, three-in-one anti-allergic agent and alcohol moisturizer.

[0034] A toiletries comprising a hair growth composition made from the above-mentioned proanthocyanidin-enhanced hair growth formula, wherein the content of the hair growth composition in the toiletries is 0.1-10wt%, wherein the most preferred content of the hair growth composition in the toiletries is 5-10wt%.

[0035] Embodiment 1, a hair growth composition, which comprises the following raw materials in terms of mass fraction: 30% apple extract; 20% Ligustrum lucidum fruit extract, 14% Trifolium pratense flower extract, 14% Platycladus orientalis leaf extract, and 14% caffeine; the content of proanthocyanidin B2 in the apple extract is not less than 20%, the content of proanthocyanidin C1 is not less than 15%, and the content of epicatechin is not less than 30%.

[0036] Example 2, which is different from Example 1 in technical features: a hair growth composition, which includes the following raw materials in terms of mass fraction: 40% apple extract; 20% Ligustrum lucidum fruit extract, 12% Trifolium pratense flower extract, 12% Platycladus orientalis leaf extract, and 12% caffeine; the content of proanthocyanidin B2 in the apple extract is not less than 20%, the content of proanthocyanidin C1 is not less than 15%, and the content of epicatechin is not less than 30%.

[0037] Example 3, which is different from Example 1 in that: a hair growth composition, wherein the following raw materials are included in terms of mass fraction: the content of the apple extract is 35%, the content of the Ligustrum lucidum fruit extract is 19.35%, the content of the red clover flower extract is 12.95%, the content of the Platycladus orientalis leaf extract is 12.90%, the content of the caffeine is 13.45%, the content of the adenosine is 6.55%, and the content of the ginseng fruit extract is 3.05%. The content of proanthocyanidin B2 in the apple extract is not less than 20%, the content of proanthocyanidin C1 is not less than 15%, and the content of epicatechin is not less than 30%.

[0038] Preparation of hair growth composition

[0039] The hair growth composition of this embodiment has a raw material formula composed of the following components in weight fractions. As shown in Table 1,

[0040]

[0041] The hair growth composition in the above table was prepared into a 5% solution with culture medium, filtered and sterilized for later use. With hair follicle matrix cells as the test material, 0.2 ml of hair follicle matrix cell suspension with a cell density of 2×105 / ml was added to a 96-well culture plate, and then 0.2 ml of hair growth composition of No. 1-8 was added, No. 9 with only cell suspension added was used as a negative control, and culture medium was used as a blank control. For each treatment, 5 replicate wells were used, and the absorbance value was determined by the MTT method, and the absorbance was determined according to the following formula.

[0042]

[0043] The effects on the proliferation rate of hair follicle matrix cells are shown in Table 2.

[0044]

[0045] The above table shows that adding apple extract, privet fruit extract, caffeine, adenosine, red clover flower extract, cypress extract and ginseng fruit extract can increase the proliferation rate of hair follicle matrix cells. Adding seven components at the same time can significantly improve the proliferation rate of hair follicle matrix cells.

[0046] Shampoo containing hair growth composition

[0047] Prepare shampoo according to the following raw material mass percentages

[0048] Xanthan gum 0.001 kg, accounting for 0.1% in the emulsifier, ethanol 0.2 kg, accounting for 20% in the emulsifier, red clover (TRIFOLIUM PRATENSE) flower extract 0.02 kg, accounting for 2% in the emulsifier, adenosine 0.01 kg, accounting for 1% in the emulsifier, apple (MALUS PUMILA) extract 0.05 kg, accounting for 5% in the emulsifier, biotin 0.0001 kg, accounting for 0.1% in the emulsifier, Ligustrum (LIGUSTRUM LUCIDUM) fruit extract 0.03 kg, accounting for 3% in the emulsifier, cypress (BIOTA ORIENTALIS) leaf extract 0.02 kg, accounting for 2% in the emulsifier, ginseng (PANAX GINSENG) fruit extract 0.005 kg, accounting for 0.5% in the emulsifier, caffeine 0.02 kg, accounting for 2% in the emulsifier, three-in-one anti-allergy 0.002 kg, accounting for 0.2% in the emulsifier, 1,2-hexanediol 0.005 kg, accounting for 0.5% in the emulsifier.

[0049] Take 63.69 kg of purified water and put it into the emulsification pot. Add xanthan gum to the pot and stir slowly. Heat to 80℃ and stir homogenously until it is completely dissolved. Cool down. Cool down to 40℃ and add ethanol, red clover flower extract, adenosine, apple extract, biotin, Ligustrum lucidum fruit extract, Platycladus orientalis leaf extract, ginseng fruit extract, caffeine, three-in-one anti-allergy, 1,2-hexanediol in turn, stir slowly until it is completely dissolved. Let it stand for 5 hours and filter out the material.

[0050] Preparation of hair growth essence containing hair growth composition

[0051] Among them, 5 parts of the No. 1 hair growth composition of the embodiment, 5 parts of the anti-inflammatory composition of embodiment 3, 20 parts of ethanol, 1 part of biotin, 3 parts of three-in-one anti-allergic, 1.2-ethylene glycol, 2 parts of xanthan gum, and 49 parts of purified water.

[0052] In order to illustrate the superiority of this formula, proanthocyanidins were extracted from apple extracts for tissue control experiments. Effects of proanthocyanidin C1 (PCC1) on skin tissue of aged mice. In H&E staining, the number of skin appendages such as hair follicles and sebaceous glands in the skin tissue of aged mice in the oral PCC1 group and the epidermal injection PCC1 group increased significantly (in the black dotted circle). In β-Gal-stained skin tissue, oral PCC1 and epidermal injection, such as Figure 1 and Figure 2 shown.

[0053] Epicatechin, procyanidin B2 and procyanidin C1 have growth-promoting activity on mouse hair epithelial cells. (●-epicatechin; ▲-procyanidin B2; ■-procyanidin C1). When procyanidin B2 is at a concentration of 50μM, the activity is the highest, and the growth rate, i.e., the activity, exceeds 300%. The peak activity of procyanidin C1 is lower than that of procyanidin B2; secondly, epicatechin has the lowest activity.

[0054] Animal data support

[0055] In order to illustrate the effects of different doses of this formula on hair growth in aged mice, the formula was injected epidermally.

[0056] like Figure 3 As shown, compared with the control group of aged mice, the aged mice injected with higher doses of proanthocyanidin B2 had more and thicker hair regrown in their hair-deficient areas during the same period of time, indicating that the hair-growth activity of proanthocyanidins has an important effect on the hair growth of aged mice, and is also related to its dosage.

[0057] The hair growth essence prepared by the present invention was given to 78 subjects for trial use, and used with a medicated comb, 2 ml each time, once in the morning and once in the evening, for 6 consecutive months.

[0058] Patient Condition

[0059] There were 78 subjects, aged between 28 and 55 years old, including 49 males and 29 females.

[0060] Criteria

[0061] Significant improvement: Hair shedding returned to normal, with obvious hair growth in the shedding area, and regeneration reaching more than 70%. Sebum secretion was significantly reduced, and dandruff and head acne were reduced.

[0062] Effective improvement: Hair loss is significantly reduced and tends to normal, there is obvious hair growth in the shedding area, the regeneration rate reaches more than 30%, sebum secretion is significantly reduced, and dandruff and head acne are reduced;

[0063] Ineffective: No significant improvement in hair loss.

[0064] Treatment Effects

[0065] After 6 months of treatment, 28 out of 78 subjects showed significant improvement in hair loss, including 11 males and 17 females, with a total significant improvement rate of 35.9%; 42 subjects showed effective improvement, including 30 males and 10 females, with a total effective improvement rate of 53.8%; 8 subjects showed no significant improvement, including 7 males and 1 female, with a total ineffective rate of 10.3%. In addition, almost all subjects showed a significant reduction in sebum secretion, dandruff and acne, indicating that the hair growth essence of the present invention can effectively improve the health of the scalp.

[0066] Figure 4 After using the hair growth essence provided by the present invention for 2 months, the hair density of the subjects was effectively improved.

[0067] Figure 5 After using the hair growth essence provided by the present invention for 4 months, the hair density of the subjects was significantly improved.

[0068] Figure 6 After using the hair growth essence provided by the present invention for 6 months, the hair density of the subjects was significantly improved.

[0069] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A proanthocyanidin-enhanced hair growth formula, characterized in that: The following raw materials are included in terms of mass fraction: 30-40% apple extract, 18-20% privet fruit extract, 12-14% red clover flower extract, 12-14% orientalis leaf extract, and 12-14% caffeine; Adenosine 6.45-6.95%, ginseng fruit extract 3.05-3.35%; the content of proanthocyanidin B2 in the apple extract is not less than 20%, the content of proanthocyanidin C1 is not less than 15%, and the content of epicatechin is not less than 30%.

2. A proanthocyanidin-enhanced hair growth formula according to claim 1, characterized in that: Other active substances for treating and preventing hair loss are also included, including biotin skin conditioner, three-in-one anti-allergic agent and alcohol moisturizer.

3. A proanthocyanidin-enhanced hair growth formula according to claim 1, characterized in that: The content of the apple extract is 35%, the content of the privet fruit extract is 19.35%, the content of the red clover flower extract is 12.95%, the content of the oriental arborvitae leaf extract is 12.90%, the content of the caffeine is 13.45%, the content of the adenosine is 6.55%, and the content of the ginseng fruit extract is 3.05%.

4. A toiletries product, characterized in that; A hair growth composition comprising the proanthocyanidin-enhanced hair growth formula according to any one of claims 1 to 3.

5. A toiletries product according to claim 4, characterized in that: The content of the hair growth composition in toiletries is 0.1-10 wt %.

6. A toiletries product according to claim 5, characterized in that: The content of the hair growth composition in the toiletries is 5-10 wt %.

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