Natural plant liquid eyeliner containing eyelash growth components and preparation method of natural plant liquid eyeliner

Abstract

The invention relates to a natural plant eyeliner containing eyelash growth components and a preparation method of the natural plant eyeliner, and belongs to the technical field of biological products. The eyeliner contains argania spinosa kernel extract and simmondsia chinensis seed extract which are prepared through specific fermentation and enzymolysis processes, and when the eyeliner is used in cooperation with other components, the eyelash growth components can be effectively removed; the eyeliner liquid has the effects of promoting eyelash growth, enhancing eyelash vitality and reducing eyelash shedding. The argania spinosa kernel extract and the simmondsia chinensis seed extract do not affect the makeup holding effect of the eyeliner, and the argania spinosa kernel extract and the simmondsia chinensis seed extract are matched for use to achieve a good eyelash growth promoting synergistic effect, so that the length and the density of eyelashes can be greatly increased, the eyelashes are protected, the damage of cosmetics is reduced, the eyelashes are unlikely to fall off, and the eyelash density is maintained; safety and no side effect are realized.

Description

Technical Field

[0001] The invention belongs to the technical field of biological products, and in particular relates to a natural plant eyeliner liquid containing eyelash growth components and a preparation method thereof. Background Art

[0002] In today's beauty makeup, liquid eyeliner has become an important eye makeup product, which is applied to the roots of eyelashes to enhance the mystery and charm of the eyes. Traditional liquid eyeliner is mainly composed of colorants, solvents, film formers and other additives. Colorants give liquid eyeliner color, which is common in classic tones such as black and brown, as well as individual colors such as blue and green. The role of solvents is to enable colorants to be evenly dispersed and maintain the fluidity of liquid eyeliner. Film formers dry quickly after application to form a thin film, making the eyeliner more durable. Additives may include preservatives, stabilizers, etc. to ensure the quality and stability of the product.

[0003] Early liquid eyeliner products had some shortcomings, such as easy smudging, difficulty in controlling the amount used, and long drying time. In order to solve the problem of easy smudging of early liquid eyeliner, researchers introduced waterproof and anti-smudging technology. By using special film-forming agents and polymers, a strong and lasting waterproof film can be formed on the eyes to prevent the influence of sweat, tears and oil.

[0004] Given that some ingredients in some eyeliners sold on the market come into contact with the roots of eyelashes, they will affect the ecological environment of the roots of eyelashes, and will also cause the pores of eyelashes to be clogged, eye discomfort, affect the normal growth of eyelashes, and even cause eyelashes to fall off, which seriously affects the beauty of eyelashes. In addition, some people are born with sparse, short or not dense eyelashes; or in daily life, eyelashes may be damaged by makeup, improper use of eyelash curlers, perming and dyeing, etc., becoming fragile and easy to break or growing slowly. Therefore, it is necessary to develop and improve eyeliner technology, and provide an eyeliner that promotes eyelash growth to reduce irritation to the eyes and skin, the impact on eyelash health and potential harm; eyeliner that promotes eyelash growth can improve the condition of eyelashes, and eyeliner that promotes eyelash growth can promote eyelash growth and density while drawing eyeliner, helping people have a more ideal eyelash appearance; visually make the eyes look bigger and more spiritual, making the whole person look more smart and attractive; at the same time, people can also use this type of eyeliner to repair and promote eyelash growth and restore the health of eyelashes. By incorporating the effect of promoting eyelash growth into liquid eyeliner, people only need to use one product when doing eye makeup, which can not only draw exquisite eyeliner but also nourish eyelashes and promote their growth at the same time, saving time and steps and providing convenience for busy modern life. Summary of the invention

[0005] The existing eyeliners have irritation to the eyes and skin, influence on the health of eyelashes and potential harm. The present invention provides a natural plant eyeliner containing eyelash growth ingredients and a preparation method thereof. The eyeliner of the present invention contains argania spinosa kernel extract and jojoba seed extract, and is used in combination with other ingredients to make the eyeliner have the effects of promoting eyelash growth, enhancing the vitality of eyelashes, and reducing eyelash shedding; the argania spinosa kernel extract and jojoba seed extract do not affect the makeup-lasting effect of the eyeliner, and the combination of the argania spinosa kernel extract and the jojoba seed extract has a good synergistic effect in promoting eyelash growth, can greatly increase the length and density of eyelashes, protect eyelashes, reduce the damage of cosmetics, are not easy to fall off, maintain the density of eyelashes, and is safe and has no side effects. The specific technical scheme is as follows:

[0006] A natural plant eyeliner containing eyelash growth ingredients comprises the following raw materials in parts by mass: 2 to 3.5 parts of argania spinosa kernel extract, 1 to 2.5 parts of jojoba seed extract, 22 to 28 parts of film former, 6 to 10 parts of colorant, 4 to 6 parts of moisturizer, 2 to 5 parts of dispersant, 0.05 to 0.12 parts of preservative, and 60 to 80 parts of solvent; the argania spinosa kernel extract is obtained by fermenting the argania spinosa kernel with Propionibacterium fraseri and Bifidobacterium longum, followed by stepwise enzymolysis with ficin and serratia protease, to obtain an extract with a value of less than 2 kDa to 3 kDa; the jojoba seed extract is obtained by fermenting jojoba seeds with Propionibacterium fraseri and Bifidobacterium longum, followed by stepwise enzymolysis with bromelain and nattokinase, to obtain an extract with a value of less than 2 kDa to 3 kDa.

[0007] In the above-mentioned eyeliner, the preparation method of the Argania spinosa kernel extract comprises the following steps:

[0008] The kernels of Argania spinulosa are crushed, and water of 8 to 10 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid, the pH value is adjusted to 6.5 to 6.8, 0.5 to 1% of the mass of the Argania spinulosa kernels Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, anaerobic fermentation is carried out at 36 to 38 degrees Celsius for 25 to 30 hours, the pH value is adjusted to 5.5 to 6.5, the temperature is adjusted to 60 to 65 degrees Celsius, and 0.5 to 1.5% of the mass of the Argania spinulosa kernels Figs are added protease, carry out enzymolysis for 50min-80min, sterilize and inactivate the enzyme, adjust the pH value to 8.5-9.5, adjust the temperature to 37℃-40℃, add 0.5%-1.5% of the mass of serratia protease of argania spinulosa kernel, carry out enzymolysis for 60min-90min, inactivate the enzyme, adjust the pH value to 6.8-7.2, filter through a sieve to obtain a crude filtrate, filter the crude filtrate through a 2kDa-3kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa-3kDa, and freeze-dry to obtain an argania spinulosa kernel extract.

[0009] In the above-mentioned eyeliner, the preparation method of the jojoba seed extract comprises the following steps:

[0010] Jojoba seeds are crushed, and water with a mass of 8 to 10 times that of the jojoba seeds is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid to adjust the pH to 6.5 to 6.8, and 0.5% to 1% by mass of the jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is performed at 36°C to 38°C for 25h to 30h, and the pH is adjusted to 6.0 to 7.5, and the temperature is adjusted to 50°C to 60°C, and 0.5% to 1.5% by mass of the jojoba seeds of pineapple are added. Jojoba seed extract is prepared by adding 1% to 3% nattokinase by weight of jojoba seeds, performing enzymolysis for 90 min to 150 min, inactivating the enzyme, adjusting the pH to 6.8 to 7.2, filtering through a mesh to obtain a crude filtrate, filtering the crude filtrate through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and freeze-drying to obtain a jojoba seed extract.

[0011] In the above preparation method, the amount of glucose added is 0.05% to 0.15% of the mass of the base liquid; the amount of peptone added is 0.03% to 0.08% of the mass of the base liquid; the amount of potassium dihydrogen phosphate added is 0.005% to 0.008% of the mass of the base liquid; the amount of magnesium sulfate added is 0.003% to 0.005% of the mass of the base liquid; the temperature of sterilization and enzyme inactivation is 80°C to 90°C, and the time of sterilization and enzyme inactivation is 20min to 30min; the temperature of enzyme inactivation is 70°C to 80°C, and the time of enzyme inactivation is 15min to 25min; the mesh size of the sieve is 200 mesh to 300 mesh.

[0012] In the above-mentioned eyeliner liquid, the film-forming agent is one or more combinations of styrene-acrylate copolymer, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone, acrylate-ethylhexyl acrylate copolymer, acrylate-dimethylaminoethyl methacrylate copolymer; the colorant is one or more combinations of carbon black, iron red, iron yellow, iron black, and titanium dioxide.

[0013] In the above-mentioned liquid eyeliner, the liquid eyeliner also includes a preservative, and the preservative is one or more combinations of phenoxyethanol, potassium sorbate, methylparaben, and propylparaben.

[0014] In the above-mentioned eyeliner liquid, the moisturizing agent includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol:butylene glycol:sodium hyaluronate = (1-3): (1-3): (0.05-0.1).

[0015] In the above-mentioned eyeliner liquid, the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = (5-8): (1-1.5).

[0016] In the above-mentioned liquid eyeliner, the solvent is purified water.

[0017] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0018] According to the mass proportion, the solvent is heated to 75-85°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, the temperature is lowered to 38-45°C, and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

[0019] The invention provides a natural plant eyeliner liquid containing eyelash growth ingredients and a preparation method thereof, which has the following beneficial effects compared with the prior art:

[0020] 1. The eyeliner of the present invention contains argania spinosa kernel extract and jojoba seed extract, which are used in combination with other ingredients to make the eyeliner have the effects of promoting eyelash growth, enhancing eyelash vitality, and reducing eyelash shedding.

[0021] 2. The Argania spinulosa kernel extract of the present invention is obtained by fermenting Argania spinulosa kernels with Propionibacterium fischeri and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with ficin and Serratia protease, to obtain an extract with a size of less than 2 kDa to 3 kDa; the extract is rich in small molecule peptides with specific efficacy and other small molecule nutrients, is easy to absorb, protects pores, and has the following eyelash growth-promoting effects:

[0022] (1) Promote eyelash growth: Provide sufficient nutrients to eyelash follicles to support the growth and division of eyelash cells; protect hair follicle cells from free radical damage and maintain normal cell metabolism and growth function.

[0023] (2) Stimulate growth factors: stimulate hair follicle cells to produce growth factors, thereby promoting cell proliferation and differentiation, and accelerating the growth rate of eyelashes.

[0024] (3) Increase eyelash density: Activate dormant eyelash follicles and re-enter the growth cycle. It can increase the number of eyelashes and thus improve eyelash density. The ingredients in the extract can promote the regeneration and development of hair follicles by regulating the activity of hair follicle stem cells.

[0025] (4) Enhance the health of hair follicles: Improve the microenvironment of eyelash follicles and enhance the health of hair follicles. Healthy hair follicles can better support the growth of eyelashes, making eyelashes thicker.

[0026] (5) Reduce eyelash shedding: Enhance the structural stability of eyelash follicles, making them more firmly fixed. The small molecule peptide components in the extract help promote the synthesis of structural proteins such as collagen and elastin by hair follicle cells, increase the strength and toughness of hair follicles. It can prolong the growth period of eyelashes and shorten the resting period, thereby reducing the natural shedding of eyelashes.

[0027] 3. The jojoba seed extract of the present invention is obtained by fermenting jojoba seeds with Propionibacterium philippe and Bifidobacterium longum, followed by stepwise enzymolysis with bromelain and nattokinase, to obtain an extract with a size of less than 2 kDa to 3 kDa; the extract is rich in effective small molecule peptides and other water-soluble small molecule nutrients, is easy to absorb and utilize, and has the following eyelash growth-promoting effects:

[0028] (1) Promote eyelash growth: Jojoba seed extract is rich in nutrients such as vitamins, minerals and unsaturated fatty acids, which can promote the growth and division of eyelash cells, protect hair follicle cells from free radical damage, and maintain normal cell metabolism. It can promote the growth of eyelashes by affecting the activity of hormone receptors or regulating the synthesis and metabolism of hormones.

[0029] (2) Increase eyelash density: Jojoba seed extract can activate dormant hair follicle stem cells and differentiate them into new eyelash cells, thereby increasing the number and density of eyelashes.

[0030] (3) Improve the microenvironment of hair follicles: It helps to improve the microenvironment around the eyelash follicles, including increasing blood circulation, providing sufficient oxygen and nutrients, regulating pH value, etc. A good microenvironment of hair follicles is conducive to the growth and development of eyelashes and increases the density of eyelashes.

[0031] (4) Enhance the adhesion of hair follicles: The ingredients in the extract can enhance the adhesion between eyelash follicles and surrounding tissues, reducing the loss of eyelashes due to external forces or chemicals.

[0032] (5) Regulate the hair cycle: By regulating the active function of eyelash pores, prolonging the growth period, shortening the resting period and shedding period, the natural shedding of eyelashes can be reduced.

[0033] Fourth, the addition of a small amount of argania spinosa kernel extract and jojoba seed extract in the present invention does not affect the makeup-holding effect of the eyeliner, and the combination of argania spinosa kernel extract and jojoba seed extract has a good synergistic effect in promoting eyelash growth, can greatly increase the length and density of eyelashes, protect eyelashes, reduce the damage of cosmetics, are not easy to fall off, maintain the density of eyelashes, and is safe and has no side effects. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0035] Example 1

[0036] A natural plant eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by mass: 2.8 parts of Argania spinosa kernel extract, 1.6 parts of jojoba seed extract, 25 parts of film former, 8 parts of colorant, 5 parts of moisturizer, 3.5 parts of dispersant, 0.12 parts of preservative, and 70 parts of solvent. Among them, the film former is styrene-acrylate copolymer; the colorant is carbon black; the moisturizer includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = 2:2:0.07; the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = 7:1.2; the solvent is purified water.

[0037] The preparation method of the Argania spinulosa kernel extract comprises the following steps:

[0038] The kernels of Argania spinulosa are crushed, and water of 9 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid, and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, and the pH value is adjusted to 6.6; 0.8% of the mass of the Argania spinulosa kernels by mass of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is carried out at 37°C for 28 hours, and the pH value is adjusted to 6, and the temperature is adjusted to 62°C. 1% of the mass of fig protease of the Argania spinulosa kernel was added, and enzymolysis was carried out for 65 minutes, sterilized and inactivated at 85°C for 25 minutes, the pH was adjusted to 9, the temperature was adjusted to 38°C, 1% of the mass of Serratia protease of the Argania spinulosa kernel was added, and enzymolysis was carried out for 75 minutes, the enzyme was inactivated at 75°C for 20 minutes, the pH was adjusted to 7, and the crude filtrate was obtained by filtering with a 250-mesh sieve. The crude filtrate was filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and the ultrafiltrate was freeze-dried to obtain an Argania spinulosa kernel extract.

[0039] The preparation method of jojoba seed extract comprises the following steps:

[0040] Jojoba seeds were crushed, and water of 9 times the mass of jojoba seeds was added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid, and 0.004% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.7; 0.8% of the mass of jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum were added respectively, and anaerobic fermentation was carried out at 37°C for 28 hours, and the pH was adjusted to 6.8, and the temperature was adjusted to 55°C. , add 1% bromelain by weight of jojoba seeds, carry out enzymolysis for 90 minutes, sterilize and inactivate enzyme at 85°C for 25 minutes, adjust pH to 8.5, adjust temperature to 45°C, add 2% nattokinase by weight of jojoba seeds, carry out enzymolysis for 120 minutes, inactivate enzyme at 75°C for 20 minutes, adjust pH to 7, filter through 250 mesh sieve to obtain coarse filtrate, filter the coarse filtrate through 2 kDa ultrafiltration membrane to obtain ultrafiltrate below 2 kDa, and freeze-dry to obtain jojoba seed extract.

[0041] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0042] According to the mass proportion, the solvent is heated to 80° C., and then a dispersant is added and mixed evenly, then a colorant is added and mixed evenly, then a moisturizer and a film-forming agent are added and mixed evenly, the temperature is lowered to 42° C., and argania spinosa kernel extract, jojoba seed extract and a preservative are added and mixed evenly to obtain an eyeliner liquid.

[0043] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 2 billion CFU / g; the bacterial activity of Bifidobacterium longum is 1 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g. The preservative is phenoxyethanol.

[0044] Example 2

[0045] A natural plant eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by mass: 2 parts of Argania spinosa kernel extract, 1 part of jojoba seed extract, 22 parts of film formers, 6 parts of colorants, 4 parts of moisturizers, 2 parts of dispersants, 0.08 parts of preservatives, and 60 parts of solvents. Among them, the film former is styrene-acrylate copolymer; the colorant is carbon black; the moisturizer includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = 1:1:0.05; the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = 5:1; the solvent is purified water.

[0046] The preparation method of the Argania spinulosa kernel extract comprises the following steps:

[0047] The kernels of Argania spinulosa are crushed, and water 8 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH value is adjusted to 6.5, and 0.5% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinulosa kernels are added respectively, and anaerobic fermentation is carried out at 36°C for 25 hours, the pH value is adjusted to 5.5, the temperature is adjusted to 60°C, and a 0.5% of the mass of fig protease of Argania spinulosa kernel is used for enzymolysis for 50 minutes, sterilized and inactivated at 80°C for 20 minutes, the pH is adjusted to 8.5, the temperature is adjusted to 37°C, 0.5% of the mass of Serratia protease of Argania spinulosa kernel is added, the enzymolysis is carried out for 60 minutes, the enzyme is inactivated at 70°C for 15 minutes, the pH is adjusted to 6.8, filtered through a 200-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain an Argania spinulosa kernel extract.

[0048] The preparation method of jojoba seed extract comprises the following steps:

[0049] Jojoba seeds are crushed, and water of 8 times the mass of the jojoba seeds is added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid are added to the base liquid, and the pH is adjusted to 6.5; 0.5% of the mass of the jojoba seeds by mass of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is carried out at 36°C for 25 hours, and the pH is adjusted to 6.0, and the temperature is adjusted to 50°C. 0.5% bromelain by weight of jojoba seeds was added, and enzymolysis was performed for 60 minutes, sterilized and enzyme-inactivated at 80° C. for 20 minutes, the pH was adjusted to 8.0, the temperature was adjusted to 40° C., 1% nattokinase by weight of jojoba seeds was added, and enzymolysis was performed for 90 minutes, enzyme-inactivated at 70° C. for 15 minutes, the pH was adjusted to 6.8, and the mixture was filtered through a 200-mesh sieve to obtain a crude filtrate, and the crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, which was freeze-dried to obtain a jojoba seed extract.

[0050] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0051] According to the mass proportion, the solvent is heated to 75°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, the temperature is lowered to 38°C, and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

[0052] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 2 billion CFU / g; the bacterial activity of Bifidobacterium longum is 1 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g. The preservative is methylparaben.

[0053] Example 3

[0054] A natural plant eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by mass: 3.5 parts of Argania spinosa kernel extract, 2.5 parts of jojoba seed extract, 28 parts of film former, 10 parts of colorant, 6 parts of moisturizer, 5 parts of dispersant, 0.05 parts of preservative, and 80 parts of solvent. Among them, the film former is styrene-acrylate copolymer; the colorant is carbon black; the moisturizer includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = 3:3:0.1; the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = 8:1.5; the solvent is purified water.

[0055] The preparation method of the Argania spinulosa kernel extract comprises the following steps:

[0056] The kernels of Argania spinulosa are crushed, and water 10 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH value is adjusted to 6.8, and 1% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinulosa kernels are added respectively, and anaerobic fermentation is carried out at 38°C for 30 hours, the pH value is adjusted to 6.5, the temperature is adjusted to 65°C, and the Argania spinulosa kernel is added. 1.5% of the mass of Ficin by weight of the Argania spinosa kernel was added, and enzymolysis was carried out for 80 minutes, sterilized and inactivated at 90°C for 30 minutes, the pH was adjusted to 9.5, the temperature was adjusted to 40°C, 1.5% of the mass of Serratia protease by weight of the Argania spinosa kernel was added, and enzymolysis was carried out for 90 minutes, the enzyme was inactivated at 80°C for 25 minutes, the pH was adjusted to 7.2, and the crude filtrate was obtained. The crude filtrate was filtered through a 3kDa ultrafiltration membrane to obtain an ultrafiltrate below 3kDa, which was freeze-dried to obtain an Argania spinosa kernel extract.

[0057] The preparation method of jojoba seed extract comprises the following steps:

[0058] Jojoba seeds were crushed, and water 10 times the mass of the jojoba seeds was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, the pH was adjusted to 6.8, and 1% of the mass of the jojoba seeds were added to Propionibacterium freudenreichii and Bifidobacterium longum, respectively, and anaerobic fermentation was carried out at 38°C for 30 hours, the pH was adjusted to 7.5, the temperature was adjusted to 60°C, and the temperature was increased. 1.5% bromelain by weight of jojoba seeds was added, and enzymolysis was performed for 120 minutes, sterilized and enzyme-inactivated at 90° C. for 30 minutes, the pH was adjusted to 9.0, the temperature was adjusted to 50° C., 3% nattokinase by weight of jojoba seeds was added, and enzymolysis was performed for 150 minutes, enzyme-inactivated at 80° C. for 25 minutes, the pH was adjusted to 7.2, and the mixture was filtered through a 300-mesh sieve to obtain a crude filtrate, and the crude filtrate was filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, which was freeze-dried to obtain a jojoba seed extract.

[0059] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0060] According to the mass fraction, the solvent is heated to 85°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, and the temperature is lowered to 45°C, and the Argania spinosa kernel extract, the Jojoba seed extract and the preservative are added and mixed evenly to obtain the eyeliner liquid. The preservative is propylparaben.

[0061] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 3 billion CFU / g; the bacterial activity of Bifidobacterium longum is 2 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g.

[0062] Example 4

[0063] A natural plant eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by mass: 2 parts of Argania spinosa kernel extract, 2.5 parts of jojoba seed extract, 22 parts of film former, 10 parts of colorant, 4 parts of moisturizer, 5 parts of dispersant, 0.1 parts of preservative, and 60 parts of solvent. Among them, the film former is styrene-acrylate copolymer; the colorant is carbon black; the moisturizer includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = 3:1:0.1; the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = 5:1.5; the solvent is purified water.

[0064] The preparation method of the Argania spinulosa kernel extract comprises the following steps:

[0065] The kernels of Argania spinulosa are crushed, and water 8 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH value is adjusted to 6.8, and 0.5% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinulosa kernels are added respectively, and anaerobic fermentation is carried out at 38°C for 25h, the pH value is adjusted to 6.5, the temperature is adjusted to 60°C, and the addition of 1.5% of the mass of fig protease of Argania spinulosa kernel is used for enzymolysis for 50 minutes, sterilized and inactivated at 90°C for 20 minutes, the pH is adjusted to 9.5, the temperature is adjusted to 37°C, 1.5% of the mass of Serratia protease of Argania spinulosa kernel is added, the enzymolysis is carried out for 60 minutes, the enzyme is inactivated at 80°C for 15 minutes, the pH is adjusted to 7.2, filtered through a 200-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, and freeze-dried to obtain an Argania spinulosa kernel extract.

[0066] The preparation method of jojoba seed extract comprises the following steps:

[0067] Jojoba seeds were crushed, and water of 8 times the mass of the jojoba seeds was added to obtain a base liquid; 0.15% of glucose by mass of the base liquid, 0.03% of peptone by mass of the base liquid, 0.008% of potassium dihydrogen phosphate by mass of the base liquid, and 0.003% of magnesium sulfate by mass of the base liquid were added to the base liquid, and the pH was adjusted to 6.8; 0.5% of the mass of the jojoba seeds by mass of Propionibacterium freudenreichii and Bifidobacterium longum were added respectively, and anaerobic fermentation was carried out at 38°C for 25 hours, and the pH was adjusted to 7.5, and the temperature was adjusted to 50°C. 1.5% bromelain by weight of jojoba seeds was added, enzymolysis was performed for 60 minutes, sterilization and enzyme inactivation was performed at 90° C. for 20 minutes, pH was adjusted to 9.0, temperature was adjusted to 40° C., 3% nattokinase by weight of jojoba seeds was added, enzymolysis was performed for 90 minutes, enzyme inactivation was performed at 80° C. for 15 minutes, pH was adjusted to 7.2, and filtration was performed through a 200-mesh sieve to obtain a crude filtrate, the crude filtrate was filtered through a 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 3 kDa, and the jojoba seed extract was lyophilized.

[0068] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0069] According to the mass fraction, the solvent is heated to 85°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, and the temperature is lowered to 38°C, and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid. The preservative is potassium sorbate.

[0070] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 3 billion CFU / g; the bacterial activity of Bifidobacterium longum is 1 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g.

[0071] Example 5

[0072] A natural plant eyeliner containing eyelash growth ingredients, comprising the following raw materials in parts by mass: 3.5 parts of Argania spinosa kernel extract, 1 part of jojoba seed extract, 28 parts of film formers, 6 parts of colorants, 6 parts of moisturizers, 2 parts of dispersants, 0.08 parts of preservatives, and 80 parts of solvents. Among them, the film former is styrene-acrylate copolymer; the colorant is carbon black; the moisturizer includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol: butylene glycol: sodium hyaluronate = 1:3:0.05; the dispersant includes sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = 8:1; the solvent is purified water.

[0073] The preparation method of the Argania spinulosa kernel extract comprises the following steps:

[0074] The kernels of Argania spinulosa are crushed, and water 10 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH value is adjusted to 6.5, and 1% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinulosa kernels are added respectively, and anaerobic fermentation is carried out at 36°C for 30 hours, the pH value is adjusted to 5.5, the temperature is adjusted to 65°C, and the Argania spinulosa kernel is added. 0.5% of the mass of Ficin by weight of Argania spinosa kernel was added, and enzymolysis was carried out for 80 minutes. The mixture was sterilized and inactivated at 80°C for 30 minutes. The pH value was adjusted to 8.5 and the temperature was adjusted to 40°C. 0.5% of the mass of Serratia protease by weight of Argania spinosa kernel was added, and enzymolysis was carried out for 90 minutes. The mixture was inactivated at 70°C for 25 minutes. The pH value was adjusted to 6.8 and the mixture was filtered through a 300-mesh sieve to obtain a crude filtrate. The crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa. The ultrafiltrate was freeze-dried to obtain an Argania spinosa kernel extract.

[0075] The preparation method of jojoba seed extract comprises the following steps:

[0076] Jojoba seeds were crushed, and water 10 times the mass of the jojoba seeds was added to obtain a base liquid; 0.05% of glucose by mass of the base liquid, 0.08% of peptone by mass of the base liquid, 0.005% of potassium dihydrogen phosphate by mass of the base liquid, and 0.005% of magnesium sulfate by mass of the base liquid were added to the base liquid, the pH was adjusted to 6.5, and 1% of the mass of the jojoba seeds Propionibacterium freudenreichii and Bifidobacterium longum were added respectively, and anaerobic fermentation was carried out at 36°C for 30 hours, the pH was adjusted to 6.0, the temperature was adjusted to 60°C, and the temperature was increased. 0.5% bromelain by weight of jojoba seeds was added, and enzymolysis was performed for 120 minutes, sterilized and enzyme-inactivated at 80°C for 30 minutes, the pH was adjusted to 8.0, the temperature was adjusted to 50°C, 1% nattokinase by weight of jojoba seeds was added, and enzymolysis was performed for 150 minutes, enzyme-inactivated at 70°C for 25 minutes, the pH was adjusted to 6.8, and the mixture was filtered through a 300-mesh sieve to obtain a crude filtrate, and the crude filtrate was filtered through a 2 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa, which was freeze-dried to obtain a jojoba seed extract.

[0077] The method for preparing the above-mentioned natural plant eyeliner containing eyelash growth ingredients comprises the following steps:

[0078] According to the mass proportion, the solvent is heated to 75°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, the temperature is lowered to 45°C, and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

[0079] The bacterial activity of Propionibacterium freudenreichii used in this embodiment is 2 billion CFU / g; the bacterial activity of Bifidobacterium longum is 2 billion CFU / g; the enzymatic activity of ficin is 100,000 U / g; the enzymatic activity of Serratia protease is 200,000 U / g; the enzymatic activity of bromelain is 500,000 U / g; and the enzymatic activity of nattokinase is 10,000 U / g. The preservative is potassium sorbate.

[0080] Example 6

[0081] The film-forming agent is a mixture of styrene-acrylate copolymer and carboxymethyl cellulose in a mass ratio of 4:1; the colorant is a mixture of titanium dioxide, red iron oxide, yellow iron oxide and black iron oxide in a mass ratio of 3:4:6:1; the eyeliner liquid also adds 0.12 parts of preservatives such as phenoxyethanol and potassium sorbate in a mass ratio; other parameters and methods are the same as those in Example 1.

[0082] Example 7

[0083] The film-forming agent is a mixture of hydroxyethyl cellulose and acrylate-ethylhexyl acrylate copolymer in a mass ratio of 3:1; the colorant is a mixture of red iron oxide, yellow iron oxide and black iron oxide in a mass ratio of 2:4:1; the eyeliner liquid also adds 0.1 parts of preservatives such as phenoxyethanol and methylparaben in a mass ratio; other parameters and methods are the same as those in Example 1.

[0084] Example 8

[0085] The film-forming agent is a mixture of polyvinyl pyrrolidone and acrylate-dimethylaminoethyl methacrylate copolymer in a mass ratio of 2:3; the colorant is a mixture of black iron oxide, red iron oxide and yellow iron oxide in a mass ratio of 0.5:4:1; the eyeliner liquid also adds 0.08 parts of preservatives such as phenoxyethanol and propylparaben in a mass ratio; other parameters and methods are the same as those in Example 1.

[0086] Comparative Example 1

[0087] No Argania spinosa kernel extract is added to the eyeliner, and the content of the Argania spinosa kernel extract is replaced by a solvent; other parameters and methods are the same as in Example 1.

[0088] Comparative Example 2

[0089] In the preparation method of the Argania spinulosa kernel extract, the Argania spinulosa kernel is crushed, 9 times the mass of water of the Argania spinulosa kernel is added to obtain a base liquid; the pH is adjusted to 6, the temperature is adjusted to 62°C, 1% of the mass of the Argania spinulosa kernel is added with ficin, enzymolysis is performed for 65 minutes, sterilization and enzyme inactivation is performed at 85°C for 25 minutes, the pH is adjusted to 9, the temperature is adjusted to 38°C, 1% of the mass of the Argania spinulosa kernel is added with Serratia protease, enzymolysis is performed for 75 minutes, enzyme inactivation is performed at 75°C for 20 minutes, the pH is adjusted to 7, and filtration is performed with a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered using a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain the Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0090] Comparative Example 3

[0091] In the preparation method of the Argania spinulosa kernel extract, the Argania spinulosa kernel is crushed, 9 times the mass of water of the Argania spinulosa kernel is added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.6, 0.8% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of the Argania spinulosa kernel are added respectively, anaerobic fermentation is carried out at 37°C for 28 hours, the pH is adjusted to 7, and filtered through a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain the Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0092] Comparative Example 4

[0093] In the preparation method of the Argania spinulosa kernel extract, the Argania spinulosa kernel is crushed, 9 times the mass of water of the Argania spinulosa kernel is added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.6, 0.8% of Propionibacterium freudenreichii by mass of the Argania spinulosa kernel is added, anaerobic fermentation is carried out at 37°C for 28h, the pH is adjusted to 6, the temperature is adjusted to 62°C, 1% of fig protease by mass of the Argania spinulosa kernel is added, enzymolysis is carried out for 65min, sterilization and enzyme inactivation is carried out at 85°C for 25min, pH is adjusted to 7, and filtration is carried out through a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain an Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0094] Comparative Example 5

[0095] In the preparation method of the Argania spinulosa kernel extract, the Argania spinulosa kernel is crushed, 9 times the mass of water of the Argania spinulosa kernel is added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.6, 0.8% of Bifidobacterium longum by mass of the Argania spinulosa kernel is added, anaerobic fermentation is carried out at 37°C for 28h, pH is adjusted to 9, the temperature is adjusted to 38°C, 1% of Serratia protease by mass of the Argania spinulosa kernel is added, enzymolysis is carried out for 75min, enzyme is inactivated at 75°C for 20min, pH is adjusted to 7, and 250 mesh sieve is filtered to obtain a crude filtrate, the crude filtrate is filtered using a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain the Argania spinulosa kernel extract. Other parameters and methods are the same as those in Example 1.

[0096] Comparative Example 6

[0097] No jojoba seed extract is added to the eyeliner, and the content of the jojoba seed extract is replaced by a solvent; other parameters and methods are the same as in Example 1.

[0098] Comparative Example 7

[0099] In the preparation method of jojoba seed extract, jojoba seeds are crushed, water of 9 times the mass of jojoba seeds is added to obtain a base liquid; pH is adjusted to 6.8, temperature is adjusted to 55°C, 1% bromelain of the mass of jojoba seeds is added, enzymolysis is performed for 90 minutes, sterilization and enzyme inactivation is performed at 85°C for 25 minutes, pH is adjusted to 8.5, temperature is adjusted to 45°C, 2% nattokinase of the mass of jojoba seeds is added, enzymolysis is performed for 120 minutes, enzyme inactivation is performed at 75°C for 20 minutes, pH is adjusted to 7, and filtration is performed through a 250-mesh sieve to obtain a coarse filtrate, the coarse filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain a jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0100] Comparative Example 8

[0101] In the preparation method of jojoba seed extract, jojoba seeds are crushed, 9 times the mass of water of jojoba seeds are added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid, and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.7, and 0.8% of Propionibacterium freudenreichii and Bifidobacterium longum by mass of jojoba seeds are added respectively, and anaerobic fermentation is carried out at 37°C for 28 hours, the pH is adjusted to 7, and filtered through a 250-mesh sieve to obtain a coarse filtrate, the coarse filtrate is filtered using a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain a jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0102] Comparative Example 9

[0103] In the preparation method of jojoba seed extract, jojoba seeds are crushed, 9 times the mass of jojoba seeds are added to obtain a base liquid; 0.1% of glucose, 0.05% of peptone, 0.006% of potassium dihydrogen phosphate and 0.004% of magnesium sulfate are added to the base liquid, the pH is adjusted to 6.7, 0.8% of Propionibacterium freudenreichii by mass of jojoba seeds are added, anaerobic fermentation is carried out at 37°C for 28h, the pH is adjusted to 8.5, the temperature is adjusted to 45°C, 2% of nattokinase by mass of jojoba seeds is added, enzymolysis is carried out for 120min, enzyme is inactivated at 75°C for 20min, pH is adjusted to 7, and filtration is carried out through a 250-mesh sieve to obtain a coarse filtrate, the coarse filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain a jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0104] Comparative Example 10

[0105] In the preparation method of jojoba seed extract, jojoba seeds are crushed, 9 times the mass of water of jojoba seeds are added to obtain a base liquid; 0.1% of glucose by mass of the base liquid, 0.05% of peptone by mass of the base liquid, 0.006% of potassium dihydrogen phosphate by mass of the base liquid and 0.004% of magnesium sulfate by mass of the base liquid are added to the base liquid, the pH is adjusted to 6.7, 0.8% of long bifidobacterium by mass of jojoba seeds are added, anaerobic fermentation is carried out at 37°C for 28 hours, pH is adjusted to 6.8, the temperature is adjusted to 55°C, 1% of bromelain by mass of jojoba seeds is added, enzymolysis is carried out for 90 minutes, sterilization and enzyme inactivation at 85°C for 25 minutes, pH is adjusted to 7, and filtration is carried out through a 250-mesh sieve to obtain a crude filtrate, the crude filtrate is filtered through a 2kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa, and freeze-dried to obtain a jojoba seed extract. Other parameters and methods are the same as those in Example 1.

[0106] The eyeliners of Examples 1 to 5 and Comparative Examples 1 to 10 were tested.

[0107] 1. Patch test

[0108] 30 subjects (female aged 20 to 35) were selected, 0.025 g of each liquid eyeliner sample was placed in a spot tester chamber and applied to the inside of the subject's arm. The spot tester was removed after 24 hours and the skin condition was observed within 48 hours. No adverse reactions were found, and the results were safe.

[0109] 2. Animal test on eyelash growth promotion

[0110] Several Wi star rats (500g-550g in weight) were selected and randomly divided into 15 groups, with 8 rats in each group (each group used the eyeliner of each embodiment and comparative example respectively). After the rats were anesthetized, the upper eyelashes of both eyes were plucked out. The skin at the root of the upper eyelashes of the left eye of the rat was painted with eyeliner every day, and the skin at the root of the upper eyelashes of the right eye was painted with purified water; once a day, for 45 days. The average growth length of the left and right upper eyelashes was measured respectively, and the eyelash growth promotion rate of change was calculated. Eyelash growth promotion rate of change = (average growth length of the left upper eyelashes - average growth length of the right upper eyelashes) ÷ average growth length of the right upper eyelashes × 100%; the test results are shown in Table 1 below.

[0111] Table 1 Eyelash growth promotion rate of change

[0112]

[0113] It can be seen from the above results that the eyeliners of Examples 1 to 5 have a good effect of promoting the growth of rat eyelashes. From the comparison of the results of Comparative Example 1, it can be seen that without adding the Argania spinosa kernel extract, the eyelash growth-promoting effect is greatly reduced, and the Argania spinosa kernel extract has a good eyelash growth-promoting effect. From the comparison of the results of Comparative Examples 2 to 5, it can be seen that by changing the preparation method of the Argania spinosa kernel extract, omitting some fermentation and enzymolysis steps, and obtaining different product components, the eyelash growth-promoting effect of the Argania spinosa kernel extract is seriously affected. From the comparison of the results of Comparative Example 6, it can be seen that without adding the jojoba seed extract, the eyelash growth-promoting effect is greatly reduced, and the jojoba seed extract has a good eyelash growth-promoting effect. From the comparison of the results of Comparative Examples 7 to 10, it can be seen that by changing the preparation method of the jojoba seed extract, omitting some fermentation and enzymolysis steps, and obtaining different product components, the eyelash growth-promoting effect of the jojoba seed extract is seriously affected. In addition, by comparing the results of Comparative Examples 1 and 6 with the results of Example 1, it can be seen that the combination of the Argania spinosa kernel extract and the Jojoba seed extract has a good synergistic effect on promoting eyelash growth.

[0114] 3. Eyelash Growth Promotion Human Trial

[0115] 90 subjects were selected, and the subjects met the conditions: the upper eyelashes were relatively short and sparse, the density and average length of the left and right upper eyelashes were comparable, and the relative deviation was ±1%. They were divided into 15 groups, each with 6 people (each group used the eyeliner of each embodiment and comparative example respectively); every morning, the sample eyeliner was used on the upper eyelashes of the left eye, and the eyeliner without Argania spinosa kernel extract and Jojoba seed extract was used on the upper eyelashes of the right eye (i.e., the eyeliner prepared without Argania spinosa kernel extract and Jojoba seed extract in Example 1). The test was continued for 3 months, and the density and average length of the left and right upper eyelashes after use were measured; the change rate of eyelash growth promotion density of the left eye relative to the right eye was calculated, eyelash growth promotion density change rate = (left eye upper eyelash density - right eye upper eyelash density) ÷ right eye upper eyelash density × 100%; the change rate of eyelash growth promotion degree of the left eye relative to the right eye was calculated, eyelash growth promotion degree change rate = (left eye upper eyelash average length - right eye upper eyelash average length) ÷ right eye upper eyelash average length × 100%. The test results are shown in Table 2 below.

[0116] Table 2 Eyelash growth promotion test results

[0117]

[0118] It can be seen from the above results that the eyeliners of Examples 1 to 5 have a good eyelash growth promoting effect, and can effectively improve the vitality of eyelashes and reduce the shedding of eyelashes. From the comparison of the results of Comparative Example 1, it can be seen that without adding the spiny argan kernel extract, the eyelash growth promoting effect is greatly reduced, and the spiny argan kernel extract has a good eyelash growth promoting effect. From the comparison of the results of Comparative Examples 2 to 5, it can be seen that by changing the preparation method of the spiny argan kernel extract, omitting some fermentation and enzymolysis steps, the product components obtained are different, which seriously affects the eyelash growth promoting effect of the spiny argan kernel extract. From the comparison of the results of Comparative Example 6, it can be seen that without adding the jojoba seed extract, the eyelash growth promoting effect is greatly reduced, and the jojoba seed extract has a good eyelash growth promoting effect. From the comparison of the results of Comparative Examples 7 to 10, it can be seen that by changing the preparation method of the jojoba seed extract, omitting some fermentation and enzymolysis steps, the product components obtained are different, which seriously affects the eyelash growth promoting effect of the jojoba seed extract. In addition, by comparing the results of Comparative Examples 1 and 6 with the results of Example 1, it can be seen that the combination of the Argania spinosa kernel extract and the Jojoba seed extract has a good synergistic effect on promoting eyelash growth.

Claims

1. A natural plant eyeliner containing eyelash growth ingredients, characterized in that: The eyeliner comprises the following raw materials in parts by weight: 2 to 3.5 parts of Argania spinosa kernel extract, 1 to 2.5 parts of Jojoba seed extract, 22 to 28 parts of film former, 6 to 10 parts of colorant, 4 to 6 parts of moisturizer, 2 to 5 parts of dispersant, 0.05 to 0.12 parts of preservative and 60 to 80 parts of solvent; The Argania spinosa kernel extract is obtained by fermenting Argania spinosa kernels with Propionibacterium fraseri and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with ficin and Serratia protease, to obtain an extract with a value of less than 2 kDa to 3 kDa; the Jojoba seed extract is obtained by fermenting jojoba seeds with Propionibacterium fraseri and Bifidobacterium longum, followed by sequential enzymatic hydrolysis with bromelain and nattokinase, to obtain an extract with a value of less than 2 kDa to 3 kDa.

2. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The preparation method of the Argania spinulosa kernel extract comprises the following steps: The kernels of Argania spinulosa are crushed, and water of 8 to 10 times the mass of the Argania spinulosa kernels is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid, the pH value is adjusted to 6.5 to 6.8, 0.5 to 1% of the mass of the Argania spinulosa kernels Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, anaerobic fermentation is carried out at 36 to 38 degrees Celsius for 25 to 30 hours, the pH value is adjusted to 5.5 to 6.5, the temperature is adjusted to 60 to 65 degrees Celsius, and 0.5 to 1.5% of the mass of the Argania spinulosa kernels Figs are added protease, carry out enzymolysis for 50min-80min, sterilize and inactivate the enzyme, adjust the pH value to 8.5-9.5, adjust the temperature to 37℃-40℃, add 0.5%-1.5% of the mass of serratia protease of argania spinulosa kernel, carry out enzymolysis for 60min-90min, inactivate the enzyme, adjust the pH value to 6.8-7.2, filter through a sieve to obtain a crude filtrate, filter the crude filtrate through a 2kDa-3kDa ultrafiltration membrane to obtain an ultrafiltrate below 2kDa-3kDa, and freeze-dry to obtain an argania spinulosa kernel extract.

3. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The preparation method of the jojoba seed extract comprises the following steps: Jojoba seeds are crushed, and water with a mass of 8 to 10 times that of the jojoba seeds is added to obtain a base liquid; glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate are added to the base liquid to adjust the pH to 6.5 to 6.8, and 0.5% to 1% by mass of the jojoba seeds of Propionibacterium freudenreichii and Bifidobacterium longum are added respectively, and anaerobic fermentation is performed at 36°C to 38°C for 25h to 30h, and the pH is adjusted to 6.0 to 7.5, and the temperature is adjusted to 50°C to 60°C, and 0.5% to 1.5% by mass of the jojoba seeds of pineapple are added. Jojoba seed extract is prepared by adding 1% to 3% nattokinase by weight of jojoba seeds, performing enzymolysis for 90 min to 150 min, inactivating the enzyme, adjusting the pH to 6.8 to 7.2, filtering through a mesh to obtain a crude filtrate, filtering the crude filtrate through a 2 kDa to 3 kDa ultrafiltration membrane to obtain an ultrafiltrate below 2 kDa to 3 kDa, and freeze-drying to obtain a jojoba seed extract.

4. A natural plant eyeliner containing eyelash growth ingredients according to claim 2 or 3, characterized in that: The amount of glucose added is 0.05% to 0.15% of the mass of the base liquid; the amount of peptone added is 0.03% to 0.08% of the mass of the base liquid; the amount of potassium dihydrogen phosphate added is 0.005% to 0.008% of the mass of the base liquid; the amount of magnesium sulfate added is 0.003% to 0.005% of the mass of the base liquid; the temperature for sterilization and enzyme inactivation is 80°C to 90°C, and the time for sterilization and enzyme inactivation is 20min to 30min; the temperature for enzyme inactivation is 70°C to 80°C, and the time for enzyme inactivation is 15min to 25min; the mesh size of the sieve is 200 to 300 meshes.

5. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The film-forming agent is one or more combinations of styrene-acrylate copolymer, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone, acrylate-ethylhexyl acrylate copolymer, acrylate-dimethylaminoethyl methacrylate copolymer; the colorant is one or more combinations of carbon black, iron red, iron yellow, iron black, and titanium dioxide.

6. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The eyeliner also includes a preservative, which is one or more combinations of phenoxyethanol, potassium sorbate, methylparaben, and propylparaben.

7. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The moisturizing agent includes propylene glycol, butylene glycol and sodium hyaluronate, and the mass ratio is: propylene glycol:butylene glycol:sodium hyaluronate=(1-3):(1-3):(0.05-0.1).

8. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The dispersant comprises sodium polyacrylate and sodium lignin sulfonate, and the mass ratio is: sodium polyacrylate: sodium lignin sulfonate = (5-8): (1-1.5).

9. The natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The solvent is purified water.

10. A method for preparing a natural plant eyeliner containing eyelash growth ingredients, for preparing the natural plant eyeliner containing eyelash growth ingredients according to claim 1, characterized in that: The preparation method comprises the following steps: According to the mass proportion, the solvent is heated to 75-85°C, and then the dispersant is added and mixed evenly, then the colorant is added and mixed evenly, then the moisturizer and the film-forming agent are added and mixed evenly, the temperature is lowered to 38-45°C, and the argania spinosa kernel extract, jojoba seed extract and preservative are added and mixed evenly to obtain the eyeliner liquid.

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