Oral care composition containing plant extract components and preparation method thereof

Abstract

The invention relates to the technical field of oral cavity cleaning and nursing materials, and discloses an oral cavity nursing composition containing plant extract components, which comprises plant extract components, inactivated probiotics, prebiotics and fluoride. The preparation method of the toothpaste product comprises the following steps: step 1, weighing corresponding components according to a formula, adding the cortex magnoliae officinalis extract, the lactobacillus paracasei, the fructo-oligosaccharide, the sucralose, the sodium benzoate and the sodium fluoride into the deionized water, stirring to fully dissolve, then adding the glycerol and the sorbitol, and stirring uniformly to obtain a mixture 1; fully mixing silicon dioxide, cellulose gum, hydroxyethyl cellulose and sodium lauryl sulfate to obtain a mixture 2; compared with the prior art, the oral cavity cariogenic bacteria inhibiting composition has the advantages that various oral cavity cariogenic bacteria are inhibited safely and effectively by using plant extract components, and beneficial bacteria proliferation is promoted by inactivating probiotics agglutinating harmful bacteria and prebiotics, occupation in oral mucosa is increased, colonization of harmful bacteria is reduced, so that the anti-sugar effect is achieved, and caries are prevented.

Description

Technical Field

[0001] The invention relates to the technical field of oral cleaning and care products, in particular to an oral care composition containing plant extract components and a preparation method thereof. Background Art

[0002] Dental caries is a progressive lesion of the hard tissue of the teeth caused by the combined effects of multiple factors in the oral cavity, which manifests as the demineralization of inorganic matter and the decomposition of organic matter. The fourth national oral health epidemiological survey released in 2017 showed that the incidence of dental caries in children in my country is on the rise. The causes of dental caries are complex. It is generally believed that sucrose or carbohydrates enter the oral cavity and adhere to the tooth surface to form dental plaque under the action of cariogenic bacteria (Streptococcus mutans, Fusobacterium nucleatum, Porphyromonas gingivalis, etc.). Cariogenic bacteria produce a large amount of organic acids in the process of metabolizing sugar substances. Organic acids cause demineralization of the tooth surface and form cavities. The reproduction of oral bacteria will further synthesize extracellular polysaccharides and intracellular polysaccharides. Polysaccharides promote the adhesion and accumulation of bacteria on the tooth surface, and provide energy sources when exogenous sugar is lacking. More cariogenic bacteria accumulate and produce acid, aggravating the occurrence of dental caries. Therefore, to prevent dental caries, it is necessary to pay attention to controlling the intake of carbohydrates in the diet and destroying the process of cariogenic bacteria using carbohydrates to produce acid. Studies have shown that effectively controlling the number of pathogenic bacteria in the oral cavity is of great significance for preventing and treating common oral problems and diseases. However, the use of antibacterial agents or antibiotics will not only inhibit the growth of harmful bacteria, but also inhibit beneficial bacteria. At the same time, it will have side effects on the body and enhance the drug resistance of oral pathogens. Therefore, antibacterial drugs should not be abused, but a more gentle way should be used to adjust the balance of oral flora.

[0003] Magnolia officinalis is a precious traditional Chinese medicine unique to my country. It mainly grows in hilly areas such as Sichuan, Hubei, and Zhejiang. Magnolia officinalis bark extract is made from the dried bark of Magnolia officinalis, a plant of the Magnoliaceae family, extracted using supercritical CO2 technology. Magnolia officinalis extract is widely used in the fields of anti-inflammatory and anti-allergic, hormone regulation, pain control, and cardiovascular protection. The active ingredients in Magnolia officinalis extract include magnolol and magnolol, which have a significant inhibitory effect on the growth and acid production of oral cariogenic bacteria, as well as their production of glucosyltransferase, α-amylase, and α-glucosidase.

[0004] Totophenol is an active substance extracted from the core material of Toto Podocarpus using supercritical CO2 technology. The polyphenols it contains have been proven to have antibacterial and anti-microbial activity. It is a rare pure plant active ingredient that can effectively fight against mold secretions. It is a natural plant fungicide with excellent activity against Gram-negative and Gram-positive bacteria, and has high inhibitory activity against bacteria that cause tooth decay and bad breath.

[0005] Probiotics are defined as "live microorganisms that are beneficial to human health when taken in a certain amount". Inactivated probiotics can compete with pathogenic bacteria for binding sites on the mucosal surface and reduce the colonization of pathogenic bacteria. In recent years, some studies have found that inactivated probiotics can promote oral health and have certain potential for preventing and treating dental caries, periodontal disease, halitosis and Candida infection.

[0006] Prebiotics refer to organic substances that are not digested and absorbed by the host but can selectively promote the metabolism and proliferation of beneficial bacteria in the body, thereby improving the health of the host. Prebiotics can stimulate the growth and activity of beneficial bacteria while inhibiting the growth and activity of potentially harmful bacteria. They can also improve mucosal barrier function, host immunity and enhance the production of short-chain fatty acids.

[0007] Publication No. CN113576942A discloses an anti-sugar toothpaste and its preparation method, wherein enzyme preparations are added to the toothpaste to decompose sugars in the oral cavity, reduce the content of sugar substances in the oral cavity, and establish a good oral environment. However, it is difficult for enzyme preparations to maintain enzyme activity in a complex toothpaste formula. After a long shelf life, the effect of the enzyme preparations deteriorates, and the ability to resist sugar and acid is reduced or lost.

[0008] Publication No. CN117122549A discloses an anti-sugar composition containing hydrolyzed wheat protein and its application. The composition is a combination of glucose oxidase, dextranase, hydrolyzed wheat protein and fluoride. It inhibits dental plaque formation through enzymes and promotes remineralization using hydrolyzed wheat protein and fluoride.

[0009] Publication No. CN115252459A discloses an oral anti-sugar composition and its application in oral care products. In the anti-sugar composition of the invention, biological enzymes are used to decompose plaque biofilms, biological polysaccharides are used to prevent the formation of plaque biofilms, and the residence time of active substances on the tooth surface is prolonged to achieve an anti-sugar effect.

[0010] In the anti-sugar research, the above patents mostly use biological enzymes to decompose dental plaque biofilms and inhibit the formation of dental plaque, thereby preventing the occurrence of caries. However, due to their particularity, biological enzymes are easily affected by various factors such as temperature, humidity, and surfactants. Their activity is unstable during the shelf life in the toothpaste packaging, and they are reduced or even lose their activity, which does not achieve the expected effect. In addition, there are few studies that directly target oral caries-causing bacteria and reduce the use of sugar to produce acid by harmful bacteria from the source. Chlorhexidine, cetylpyridinium chloride and other antibacterial agents are also mostly used to inhibit harmful bacteria. Long-term use will destroy the structure of the oral flora and is not conducive to oral health. There is no relevant data research on the use of natural and safe plant extracts to inhibit harmful oral bacteria and coordinate with inactivated probiotics to regulate the oral flora. Summary of the invention

[0011] The technical problem to be solved by the present invention is to overcome the above technical difficulties and provide an oral care composition containing plant extract ingredients and a preparation method thereof. The present invention uses plant extract ingredients to safely and effectively inhibit a variety of oral cariogenic bacteria, and agglutinate harmful bacteria by inactivating probiotics. Prebiotics promote the proliferation of beneficial bacteria, increase the space occupied in the oral mucosa, and reduce the colonization of harmful bacteria, thereby achieving an anti-sugar effect and preventing the occurrence of caries.

[0012] In order to solve the above technical problems, the technical solution provided by the present invention is:

[0013] An oral care composition containing plant extract ingredients, including plant extract ingredients, inactivated probiotics, prebiotics, and fluoride;

[0014] Calculated by weight percentage: plant extract is 0.05% to 1%, inactivated probiotics is 0.05% to 3%, prebiotics is 0.1% to 3%, and fluoride is 0.2% to 0.8%.

[0015] Furthermore, the plant extract ingredient includes any one of Magnolia officinalis bark extract and tocopherol; the inactivated probiotics include one or more of Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus salivarius, and Lactobacillus rhamnosus; the prebiotics include one or more of fructooligosaccharides, xylo-oligosaccharides, inulin, and arginine; the fluoride includes one or more of sodium fluoride, sodium monofluorophosphate, and olaflurox.

[0016] Furthermore, the oral care product to which the oral care composition is applied includes any one of toothpaste, tooth powder, tooth tablets, and mouthwash.

[0017] Furthermore, the toothpaste product includes the following components in weight percentage: 0.05% to 1% plant extract, 0.05% to 3% inactivated probiotics, 0.1% to 3% prebiotics, 0.5% to 0.8% fluoride, 20% to 50% abrasive, 0.3% to 1.2% thickener, 1% to 2.5% surfactant, 0.1% to 0.3% sweetener, 0.1% to 1% preservative, 30% to 60% moisturizer, 0.4% to 1% flavor, and the balance is water.

[0018] Furthermore, the mouthwash product includes the following components in weight percentage: 0.05% to 0.1% plant extract, 0.05% to 0.3% inactivated probiotics, 0.1% to 0.3% prebiotics, 0.2% to 0.6% fluoride, 0.3% to 1.2% thickener, 0.4% to 1.2% surfactant, 0.1% to 0.3% sweetener, 0.1% to 1% preservative, 5% to 20% moisturizer, 0.1% to 0.5% flavor, and the balance is water.

[0019] Furthermore, the abrasive agent is one or more of silicon dioxide, calcium carbonate, and calcium hydrogen phosphate dihydrate.

[0020] Furthermore, the thickener is one or more of cellulose gum, hydroxypropyl cellulose, xanthan gum, and carbomer.

[0021] Further, the surfactant is one or more of sodium lauryl sulfate, sodium lauroyl sarcosinate, and PEG-40 hydrogenated castor oil;

[0022] The sweetener is one or more of sucralose, steviol glycoside, and saccharin sodium.

[0023] Furthermore, the preservative is one or more of sodium benzoate, p-hydroxyacetophenone, 1,2-hexanediol, and o-cymene-5-ol;

[0024] The moisturizing agent is one or more of sorbitol, glycerol, polyethylene glycol-8 and propylene glycol.

[0025] The preparation method of the oral care composition containing plant extract ingredients, the preparation of the toothpaste product comprises the following steps:

[0026] Step 1, weighing the corresponding ingredients according to the formula, adding Magnolia officinalis bark extract, Lactobacillus paracasei, oligofructose, sucralose, sodium benzoate, and sodium fluoride into deionized water, stirring to fully dissolve, and then adding glycerol and sorbitol, stirring evenly to obtain mixture 1; fully mixing silicon dioxide, cellulose gum, hydroxyethyl cellulose, and sodium lauryl sulfate to obtain mixture 2;

[0027] Step 2: Pump mixture 1 into the main machine, stir at high speed under vacuum for 10 to 15 minutes, then pump mixture 2 into the main machine, mix and disperse thoroughly under vacuum for 15 to 20 minutes, add flavor, mix under vacuum for 5 to 10 minutes, and obtain the toothpaste product.

[0028] The advantages of the present invention compared with the prior art are:

[0029] 1. The present invention is designed for the oral cavity and uses natural plant extracts to specifically inhibit the growth of oral caries-causing bacteria. The reduction of oral caries-causing bacteria directly affects the acid-producing demineralization process, slows down the decrease in oral pH, and promotes remineralization;

[0030] 2. The present invention uses a combination of multiple inactivated probiotics and prebiotic ingredients such as oligofructose, which can effectively reduce the colonization of harmful bacteria in the oral cavity and further reduce the reproduction of harmful bacteria in the oral cavity;

[0031] 3. The present invention adds fluoride to promote tooth remineralization, which is internationally recognized as the best ingredient for preventing caries. Fluoride can replace the hydroxyl group in hydroxyapatite in the enamel structure to form fluoroapatite, thereby reducing the solubility of the enamel surface and enhancing resistance to acid.

[0032] 4. The present invention uses natural extracts to inhibit the growth of oral cariogenic bacteria, adds inactivated probiotics and prebiotics to promote the establishment of a good oral microenvironment, and adds fluoride to enhance the remineralization of tooth enamel, thereby enhancing the oral cavity's resistance to sugar and acid from multiple channels and directions to avoid the occurrence of caries. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 1 is a graph showing changes in pH of culture solutions in comparison between various embodiments of the present invention and various comparative examples. DETAILED DESCRIPTION

[0034] In the description of the present invention, unless otherwise specified, "plurality" means two or more. In addition, the term "include" and any variations thereof are intended to cover non-exclusive inclusions.

[0035] The present invention is further described in detail below in conjunction with the implementation modes and the accompanying drawings.

[0036] Example 1

[0037] The present embodiment provides an application of an oral care composition in a toothpaste, comprising the following components in parts by weight: 0.05 parts of Magnolia officinalis bark extract, 0.05 parts of Lactobacillus paracasei, 0.05 parts of oligofructose, 0.15 parts of sucralose, 0.6 parts of cellulose gum, 0.15 parts of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 parts of flavor, 18 parts of silicon dioxide, 0.3 parts of sodium benzoate, 2 parts of sodium lauryl sulfate, 0.14 parts of sodium fluoride, and the balance being water.

[0038] The preparation method of the toothpaste of this embodiment comprises the following steps:

[0039] Step 1, weighing the corresponding ingredients according to the formula, adding Magnolia officinalis bark extract, Lactobacillus paracasei, oligofructose, sucralose, sodium benzoate, and sodium fluoride into deionized water, stirring to fully dissolve, and then adding glycerol and sorbitol, stirring evenly to obtain a mixture 1;

[0040] Thoroughly mix silicon dioxide, cellulose gum, hydroxyethyl cellulose and sodium lauryl sulfate to obtain mixture 2;

[0041] Step 2: Pump mixture 1 into the main machine, stir at high speed under vacuum for 10-15 minutes, then pump mixture 2 into the main machine, mix and disperse thoroughly under vacuum for 15-20 minutes, add flavor, mix under vacuum for 5-10 minutes, and obtain the toothpaste of this embodiment.

[0042] Example 2

[0043] The present embodiment provides an application of an oral care composition in a toothpaste, comprising the following components in parts by weight: 0.1 part of Magnolia officinalis bark extract, 0.2 part of Lactobacillus paracasei, 0.2 part of Lactobacillus acidophilus, 0.1 part of Lactobacillus salivarius, 0.1 part of oligofructose, 0.15 part of sucralose, 0.6 part of cellulose gum, 0.15 part of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 part of flavor, 18 parts of silicon dioxide, 0.3 part of sodium benzoate, 0.03 part of o-cymene-5-ol, 2 parts of sodium lauroyl sarcosinate, 0.63 part of sodium monofluorophosphate, and the balance being water.

[0044] The preparation method of this embodiment is the same as that of embodiment 1.

[0045] Example 3

[0046] The present embodiment provides an application of an oral care composition in a toothpaste, comprising the following components in parts by weight: 0.1 part of Magnolia officinalis bark extract, 0.1 part of totophenol, 0.5 part of Lactobacillus paracasei, 0.5 part of Lactobacillus acidophilus, 0.3 part of Lactobacillus salivarius, 0.3 part of oligofructose, 0.05 part of arginine, 0.2 part of saccharin sodium, 0.6 part of cellulose gum, 0.15 part of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 part of flavor, 20 parts of silicon dioxide, 0.5 part of p-hydroxyacetophenone, 0.5 part of 1,2-hexanediol, 0.03 part of o-cymene-5-ol, 0.63 part of sodium monofluorophosphate, 0.05 part of olaflur, 2 parts of sodium lauroyl sarcosinate, and the balance being water.

[0047] The preparation method of this embodiment is the same as that of embodiment 1.

[0048] Example 4

[0049] The present embodiment provides an application of an oral care composition in a toothpaste, comprising the following components in parts by weight: 0.15 parts of a magnolia bark extract, 0.1 parts of totophenol, 0.2 parts of Lactobacillus paracasei, 0.2 parts of Lactobacillus acidophilus, 0.1 parts of Lactobacillus salivarius, 0.5 parts of oligofructose, 0.1 parts of arginine, 0.1 parts of inulin, 0.15 parts of sucralose, 0.6 parts of cellulose gum, 0.15 parts of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 parts of flavor, 20 parts of silicon dioxide, 0.3 parts of sodium benzoate, 0.05 parts of o-cymene-5-ol, 2 parts of sodium lauroyl sarcosinate, 0.78 parts of sodium monofluorophosphate, 0.1 parts of olafluor, and the balance being water.

[0050] The preparation method of this embodiment is the same as that of embodiment 1.

[0051] Comparative Example 1

[0052] The ordinary toothpaste described in the comparative example includes the following components in parts by weight: 0.15 parts of sucralose, 0.6 parts of cellulose gum, 0.15 parts of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 parts of flavor, 20 parts of silicon dioxide, 0.3 parts of sodium benzoate, 2 parts of sodium lauryl sulfate, and the balance is water.

[0053] The preparation method of this comparative example is the same as that of Example 1.

[0054] Comparative Example 2

[0055] The ordinary toothpaste described in the comparative example comprises the following components in parts by weight: 0.2 parts of Lactobacillus paracasei, 0.15 parts of sucralose, 0.6 parts of cellulose gum, 0.15 parts of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 parts of flavor, 20 parts of silicon dioxide, 0.3 parts of sodium benzoate, 2 parts of sodium lauroyl sarcosinate, and the balance is water.

[0056] The preparation method of this comparative example is the same as that of Example 1.

[0057] Comparative Example 3

[0058] The ordinary toothpaste described in the comparative example includes the following components in parts by weight: 0.5 parts of oligofructose, 0.15 parts of sucralose, 0.6 parts of cellulose gum, 0.15 parts of hydroxyethyl cellulose, 60 parts of sorbitol, 3 parts of glycerol, 0.5 parts of flavor, 20 parts of silicon dioxide, 0.3 parts of sodium benzoate, 2 parts of sodium lauryl sulfate, and the balance is water.

[0059] The preparation method of this comparative example is the same as that of Example 1.

[0060] (1) The toothpaste products of the embodiment and the comparative example were placed at room temperature, 45°C, and -18°C to test their stability. The results are as follows:

[0061]

[0062] (2) Toothpaste antibacterial test: The toothpaste antibacterial test method refers to WS / T650-2019 "Antibacterial and antibacterial effect evaluation method" 5.1.1 Suspension quantitative antibacterial test, and the strain selected is Streptococcus mutans CICC10387. The antibacterial rate of the embodiment and the comparative example is measured.

[0063] sample Antibacterial rate (mean) Example 1 78.79 Example 2 88.52 Example 3 98.48 Example 4 ＞99.9 Comparative Example 1 11.26 Comparative Example 2 35.96 Comparative Example 3 31.25

[0064] Judgment criteria: antibacterial rate ≥ 50%-90%, antibacterial effect, antibacterial rate ≥ 90%, strong antibacterial effect. It can be seen from the table that the examples all have good antibacterial effect on Streptococcus mutans, with Example 4 having the best antibacterial effect, and the comparative example ordinary toothpaste has no obvious antibacterial effect on Streptococcus mutans, and the data is in a messy state.

[0065] (3) Toothpaste sugar acid resistance test: The experiment mixes the sample with the saliva in the mouth, conducts bacterial culture for 48 hours, measures the pH value of the culture solution, and analyzes whether the sample can inhibit the growth of cariogenic bacteria in the mouth based on the trend of pH value changes. The test steps are: ① Prepare liquid thioglycollate culture solution and toothpaste slurry; ② Collect fresh human saliva; ③ Mix the saliva and toothpaste slurry in proportion, take the supernatant quantitatively after centrifugation and add it to the culture solution and culture it at 37℃ for 48 hours as sample group 1, during which the pH value of the culture solution is regularly measured. ④ Simultaneously use the culture solution containing 1% sucrose as sample group 2 and the culture solution containing 1% lactose as sample group 3, and conduct two groups of comparative experiments according to the above steps.

[0066]

[0067] The negative control is treated with deionized water, and it can be seen that the acid produced by pathogenic bacteria causes the pH to decrease. By comparing the embodiments, it can be concluded that the anti-sugar and acid effect of using a combination of plant extracts and inactivated probiotics and prebiotics will be better than using a single channel, and the effect will be better when the amount used is large. However, it can be seen from the comparative example that ordinary toothpaste does not have an anti-sugar and acid effect.

[0068] The present invention and its embodiments are described above, and such description is not restrictive. If a person skilled in the art is inspired by it and designs an embodiment similar to the technical solution without creativity without departing from the purpose of the invention, it shall fall within the protection scope of the present invention.

Claims

1. An oral care composition containing a plant extract, characterized in that: Including plant extracts, inactivated probiotics, prebiotics, and fluoride; Calculated by weight percentage: plant extract is 0.05% to 1%, inactivated probiotics is 0.05% to 3%, prebiotics is 0.1% to 3%, and fluoride is 0.2% to 0.8%.

2. The oral care composition containing plant extract ingredients according to claim 1, characterized in that: The plant extract component includes any one of Magnolia officinalis bark extract and totarol; the inactivated probiotics include one or more of Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus salivarius, and Lactobacillus rhamnosus; the prebiotics include one or more of fructooligosaccharides, xylo-oligosaccharides, inulin, and arginine; the fluoride includes one or more of sodium fluoride, sodium monofluorophosphate, and olaflurox.

3. The oral care composition containing plant extract ingredients according to claim 2, characterized in that: The oral care product to which the oral care composition is applied includes any one of toothpaste, tooth powder, tooth tablets and mouthwash.

4. The oral care composition containing plant extract ingredients according to claim 3, characterized in that: The toothpaste product comprises the following components in percentage by weight: 0.05% to 1% of plant extract, 0.05% to 3% of inactivated probiotics, 0.1% to 3% of prebiotics, 0.5% to 0.8% of fluoride, 20% to 50% of abrasive, 0.3% to 1.2% of thickener, 1% to 2.5% of surfactant, 0.1% to 0.3% of sweetener, 0.1% to 1% of preservative, 30% to 60% of moisturizer, 0.4% to 1% of flavor, and the balance is water.

5. The oral care composition containing plant extract ingredients according to claim 3, characterized in that: The mouthwash product comprises the following components in percentage by weight: 0.05% to 0.1% of plant extract, 0.05% to 0.3% of inactivated probiotics, 0.1% to 0.3% of prebiotics, 0.2% to 0.6% of fluoride, 0.3% to 1.2% of thickener, 0.4% to 1.2% of surfactant, 0.1% to 0.3% of sweetener, 0.1% to 1% of preservative, 5% to 20% of moisturizer, 0.1% to 0.5% of flavor, and the balance is water.

6. The oral care composition containing plant extract ingredients according to claim 4 or 5, characterized in that: The abrasive agent is one or more of silicon dioxide, calcium carbonate, and calcium hydrogen phosphate dihydrate.

7. The oral care composition containing plant extract ingredients according to claim 4 or 5, characterized in that: The thickener is one or more of cellulose gum, hydroxypropyl cellulose, xanthan gum and carbomer.

8. The oral care composition containing plant extract ingredients according to claim 4 or 5, characterized in that: The surfactant is one or more of sodium lauryl sulfate, sodium lauroyl sarcosinate, and PEG-40 hydrogenated castor oil; The sweetener is one or more of sucralose, steviol glycoside, and saccharin sodium.

9. The oral care composition containing plant extract ingredients according to claim 4 or 5, characterized in that: The preservative is one or more of sodium benzoate, p-hydroxyacetophenone, 1,2-hexanediol, and o-cymene-5-ol; The moisturizing agent is one or more of sorbitol, glycerol, polyethylene glycol-8 and propylene glycol.

10. The method for preparing an oral care composition containing plant extract ingredients according to any one of claims 1 to 4 and 6 to 9, characterized in that: The preparation of the toothpaste product comprises the following steps: Step 1, weighing the corresponding ingredients according to the formula, adding Magnolia officinalis bark extract, Lactobacillus paracasei, oligofructose, sucralose, sodium benzoate, and sodium fluoride into deionized water, stirring to fully dissolve, and then adding glycerol and sorbitol, stirring evenly to obtain mixture 1; fully mixing silicon dioxide, cellulose gum, hydroxyethyl cellulose, and sodium lauryl sulfate to obtain mixture 2; Step 2: Pump mixture 1 into the main machine, stir at high speed under vacuum for 10 to 15 minutes, then pump mixture 2 into the main machine, mix and disperse thoroughly under vacuum for 15 to 20 minutes, add flavor, mix under vacuum for 5 to 10 minutes, and obtain the toothpaste product.

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