Bacteriostatic compound, female private detergent and application

Abstract

The invention provides an antibacterial compound, a female private washing agent and application, and relates to the technical field of daily chemical washing care products. According to the antibacterial compound and the female private washing agent, the GML can be applied to the female private washing agent of a water-based system by utilizing the emulsifying and solubilizing effects of the solubilizer on the GML, and the GML and the natural lactic acid are compounded to achieve a synergistic effect, so that a good antibacterial effect is achieved under the condition that the dosage is relatively small; according to the present invention, the antibacterial rate on Escherichia coli, Staphylococcus aureus and Candida albicans (Candida albicans) is more than 99%, and no inhibition effect on vagina Lactobacillus mucilaginosus exists, such that the bacterial colony balance of the vagina micro-ecological environment Lactobacillus flora can be maintained, and the good antibacterial effect on harmful bacteria can be provided;

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical cleaning products, and in particular to an antibacterial compound, a female private detergent and applications thereof. Background Art

[0002] According to the scientific and technological research related to women's health, we will strengthen the research on the main influencing factors and intervention measures of women's health, and promote new technologies to promote women's health. Due to the huge life pressure of modern urban women, coupled with irregular work and rest eating habits and serious environmental pollution problems, which lead to a decrease in resistance and the body is often in a sub-healthy state. More and more women are beginning to face up to the health problems of private parts, realizing that private health care should not be a topic to be ashamed of, but a normal health care need; and as contemporary women pay more attention to their own physical health, as a daily cleaning and care product, the market size of female cleaning care liquid has shown a steady upward trend in recent years, reaching 32.27 billion yuan in 2022, and is expected to reach 48.49 billion yuan in 2027, with a growth rate of 10.4%.

[0003] Through consumer surveys, it was found that the main efficacy claims of female cleaning care liquids are basic cleaning, antibacterial and antibacterial, odor removal, itching treatment, acid-base balance, etc. However, due to the special nature of women's private parts, the internal flora is diverse and huge, and they coexist and antagonize each other. Under normal circumstances, the vaginal flora is composed of more than 50 anaerobic, aerobic, facultative anaerobic microorganisms, as well as protozoa, viruses, and mycoplasmas. Among them, anaerobic microorganisms are mainly lactobacilli, bacteroides, Gardnerella, bifidobacteria, etc.; aerobic microorganisms include enterococci, etc.; facultative anaerobic microorganisms are: streptococci, Escherichia coli, Staphylococcus, Corynebacterium, Candida albicans, etc. Among them, lactobacilli account for about 95%, which is the main resident bacteria in the normal female vaginal microecological environment. Lactobacilli metabolize to produce lactic acid, hydrogen peroxide, bacteriocins, bacteriocin-like bacteria and biosurfactants, etc. The metabolites of lactobacilli metabolism can inhibit the growth of pathogenic microorganisms and maintain the ecological balance of vaginal microorganisms in the body. Generally, when using conventional anti- / antibacterial washes, while inhibiting harmful bacterial microbial flora, it is easy to destroy the beneficial lactobacillus flora in the body, thereby disrupting the balance of the vaginal microbial ecology in the body, causing the product to have some side effects on the human body during use. Summary of the invention

[0004] In order to inhibit harmful bacterial microbial flora in the vagina of women, maintain the balance of vaginal microbial ecology, and provide a stable water-based female intimate washing liquid, the present invention provides an antibacterial compound, a female intimate washing agent and application.

[0005] In a first aspect, the present application provides an antibacterial compound:

[0006] An antibacterial compound comprises, by weight, 0.3-0.5 parts of an antibacterial composition, 1 part of PEG-40 hydrogenated castor oil and 98.5-98.7 parts of water, wherein the combined antibacterial agent comprises lauric acid monoglyceride and lactic acid, and the mass ratio of lauric acid monoglyceride to lactic acid is (0.1-0.3):(0.2-0.4).

[0007] In the second aspect, the present application provides a female private detergent:

[0008] A female private detergent, comprising the following raw materials in parts by weight:

[0009]

[0010] The antibacterial composition is lauric acid monoglyceride and lactic acid in a mass ratio of (0.1-0.3):(0.2-0.4).

[0011] Furthermore, the female intimate detergent comprises the following raw materials in parts by mass:

[0012]

[0013] The antibacterial composition is lauric acid monoglyceride and lactic acid in a mass ratio of (0.1-0.3):(0.2-0.4).

[0014] Monolaurin (GML for short) can significantly inhibit vaginal Candida and Gardnerella, but has no effect on the dominant bacteria genus Lactobacillus. GML will not inhibit the production of vaginal Lactobacillus in macaques, and will not change the mucosal flora and integrity. Long-term use of GML is conducive to the growth of Lactobacillus and can better maintain the balance of the body's microbial ecology, such as the vagina. At the same time, monolaurin (GML for short) and natural lactic acid can synergistically enhance their effectiveness and show a better antibacterial effect.

[0015] GML is also included in the 2021 version of the list of used cosmetic raw materials. The antibacterial properties and safety of GML are recognized, but there are not many cases of GML being used in women's intimate cleaning and care products. The main reason is that GML has poor solubility in water-based products, which makes it impossible to use GML in water-based women's intimate washes.

[0016] The antibacterial compound and female private parts detergent provided by the present invention can be used for daily cleaning and care of female private parts, and have obvious advantages in maintaining the colony balance of lactobacillus flora and skin irritation.

[0017] After a lot of experimental research and exploration, the inventor's team found that the solubility of GML in water-based system products can be increased by using solubilizers and emulsifiers, especially when the solubilizer is PEG-40 hydrogenated castor oil, the effect is more significant, so that GML can be applied to female intimate washes, while maintaining the balance of lactobacillus colonies and exerting its good antibacterial effect.

[0018] Furthermore, the surfactant includes one or more combinations of sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine, coconut oil monoethanolamide, amino acid surfactant, sodium lauroyl amphoacetate, and sodium cocoyl isethionate.

[0019] Furthermore, the moisturizing agent includes one or a combination of glycerin, propylene glycol, hyaluronic acid, and dextran; and the chelating agent includes tetrasodium glutamate diacetate or disodium ethylenediaminetetraacetate.

[0020] Furthermore, the viscosity modifier includes one or more combinations of hydroxyethyl cellulose, carboxymethyl cellulose, xanthan gum, and nonionic guar gum.

[0021] Furthermore, the viscosity of the female intimate detergent is 7000mPa·s to 10000mPa·s.

[0022] Furthermore, the pH buffer regulator includes citric acid and / or sodium citrate; the preservative includes a combination of one or more of sodium benzoate, potassium sorbate, and phenoxyethanol.

[0023] Furthermore, the female private detergent has an inhibition rate of not less than 99% against Escherichia coli, Staphylococcus aureus and Candida albicans, and an inhibition rate of not more than 5% against vaginal mucus Lactobacillus.

[0024] In the third aspect, the present application provides the use of an antibacterial compound or a female private detergent:

[0025] An application of an antibacterial compound or a female intimate detergent, and an application of the antibacterial compound or the female intimate detergent in preparing an external vaginal care detergent for maintaining the balance of vaginal lactobacilli.

[0026] Beneficial effects:

[0027] 1) The antibacterial composition and the female intimate detergent of the present invention use lauric acid monoglyceride (GML for short) and natural lactic acid in combination, and the two can synergistically enhance the effect and show a relatively good antibacterial effect. At the same time, GML will not change the mucosal flora and integrity, and has a certain protective effect on the growth of lactobacilli, which can better maintain the balance of microbial ecology in the body.

[0028] 2) The antibacterial composition and female intimate detergent of the present invention utilize the emulsifying and solubilizing effect of the solubilizing agent on GML, so that GML can be applied to female intimate washes of a water-based system, and GML and natural lactic acid are compounded to play a synergistic role. When used in a small amount, they have a good antibacterial effect, with an antibacterial rate of more than 99% against Escherichia coli, Staphylococcus aureus, and Candida albicans, and have basically no inhibitory effect on vaginal mucus lactobacilli, and can maintain the colony balance of lactobacilli in the vaginal microecological environment, showing a good antibacterial effect on harmful bacteria. DETAILED DESCRIPTION

[0029] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments.

[0030] In order to make the technical means, creative features, purpose and efficacy of the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. The experimental methods in the following embodiments, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels.

[0031] In the following embodiments, the following abbreviations will be used and have the functions indicated.

[0032] Lauric acid monoglyceride, also known as lauric acid monoglyceride, referred to as GML, has a molecular formula of C 15 H 30 O4, molecular weight is 274.21, HLB is 5.2.

[0033] Natural lactic acid, scientifically known as a-hydroxypropionic acid, is a naturally occurring organic acid with a purity of 80%, a molecular formula of C3H6O3, and a molecular weight of 90.08.

[0034] PEG-40 hydrogenated castor oil, an industrial product, is ethoxylated hydrogenated castor oil. 40 represents the molar number of ethylene oxide. It is a non-ionic solubilizer.

[0035] AES: sodium polyoxyethylene fatty alcohol ether sulfate, the carbon chain length of the fatty alcohol is 12-14, the average EO number is 2, and the purity is 70%.

[0036] Cocamidopropyl betaine, referred to as CAB, with a purity of 35%, is an amphoteric surfactant.

[0037] Coconut oil monoethanolamide, referred to as CMEA, is a non-ionic surfactant.

[0038] Amino acid surfactants, including: sodium cocoyl glutamate, sodium cocoyl alanine, sodium lauroyl sarcosinate, sodium lauroyl aspartate.

[0039] Sodium lauroamphoacetate, amphoteric surfactant, industrial product, purity 35%.

[0040] Screening of solubilizers for lauric acid monoglyceride:

[0041] Preparation Example 1 contains 0.1% lauric acid monoglyceride + 1% propylene glycol + deionized water.

[0042] Preparation Example 2 contains 0.1% lauric acid monoglyceride + 1% AEO-3 + deionized water.

[0043] Preparation Example 3 contains 0.1% lauric acid monoglyceride + 1% PEG-7 glyceryl cocoate + deionized water.

[0044] Preparation Example 4 contains 0.1% lauric acid monoglyceride + 1% PEG-40 hydrogenated castor oil + deionized water.

[0045] The appearance and stability of the mixtures of Preparation Examples 1 to 4 were tested, and it was found that the stability of the samples of Preparation Examples 1 to 3 was poor, and turbidity or precipitation would occur during the storage process. The stability of the sample of Preparation Example 4 was better, and it was clear and transparent during the inspection process without turbidity or precipitation. PEG-40 hydrogenated castor oil was selected as the solubilizer for lauric acid monoglyceride.

[0046] Example 1, a female private detergent, is prepared by weighing the components according to the raw material dosages in Table 1, stirring at room temperature to achieve a uniform reaction.

[0047] Example 2 is Example 4, a female intimate detergent. The difference from Example 1 is that the dosage of raw materials in the female intimate detergent is set differently, as shown in Table 1.

[0048] The antibacterial composition in Example 1 comprises 0.1% lauric acid monoglyceride + 0.2% natural lactic acid.

[0049] The antibacterial composition in Example 2 comprises 0.2% lauric acid monoglyceride + 0.2% natural lactic acid.

[0050] The antibacterial composition in Example 3 comprises 0.2% lauric acid monoglyceride + 0.3% natural lactic acid.

[0051] The antibacterial composition in Example 4 comprises 0.1% lauric acid monoglyceride + 0.4% natural lactic acid.

[0052] Table 1. List of raw material dosages of female intimate detergents from Example 1 to Example 4

[0053]

[0054]

[0055] In other embodiments, the amount of citric acid is in the range of 0-0.3 parts and is added according to actual needs.

[0056] Example 5 is a female intimate detergent, which is different from Example 1 in that PEG-7 glyceryl cocoate is used to replace PEG-40 hydrogenated castor oil in an equal amount.

[0057] Example 6, a female intimate detergent, is different from Example 1 in that AEO-3 is used to replace PEG-40 hydrogenated castor oil in an equal amount.

[0058] Performance testing:

[0059] Determination of pH: The composition was prepared into a sample solution with a mass fraction of 1%, and tested with a pH meter at 25°C.

[0060] Stability test: Refer to the "Disinfection Technical Specifications" 2002 for the stability test of disinfection products, and the storage validity period is 12 months. For appearance stability, store at -5℃ and 54℃ for 14 days to observe whether there is precipitation or suspended matter on the appearance. If there is no precipitation or suspended matter, the sample is recorded as "qualified"; if there is precipitation or suspended matter, the sample is recorded as "unqualified".

[0061] Stability test of antibacterial efficacy: the sample was stored in a 54°C oven for 14 days, and then the content of the active ingredient of the female intimate detergent was detected. The decrease rate of the active ingredient of the antibacterial agent in the sample was ≤10%, which was recorded as "qualified", and the decrease rate of the active ingredient of the antibacterial agent in the sample was >10%, which was recorded as "unqualified". Table 2 shows the pH value, appearance stability, and stability of the antibacterial agent in the female intimate detergent of Examples 1 to 9, wherein: X1 is the content of the active ingredient of the antibacterial agent in the sample just prepared, X2 is the content of the active ingredient of the antibacterial agent after the sample was placed in a 54°C oven for 14 days, and the test of the active ingredient of the antibacterial agent is carried out by liquid chromatography and quantitative analysis; antibacterial agent decrease rate = (X1-X2) / X1*100%.

[0062] Table 2. Experimental results of pH value, appearance stability and antibacterial agent reduction rate of female private detergents of Examples 1-6

[0063]

[0064] In the female intimate detergents of Examples 5 and 6, conventional lauric acid monoglyceride solubilizers PEG-7 glyceryl cocoate and AEO-3 were used in turn, and the prepared female intimate detergents had obvious suspended matter, indicating that the PEG-40 hydrogenated castor oil used in the female intimate detergent of the present application has a good compatibility effect with lauric acid monoglyceride, lactic acid and surfactant, and a more stable female intimate detergent can be prepared, which is beneficial to maintaining the balance of the vaginal microbial ecology in the user's body and enhancing the antibacterial effect.

[0065] Comparative Example 1 is a female intimate detergent, which differs from Example 4 in that no antibacterial composition is added.

[0066] Comparative Example 2 is a female intimate detergent, which is different from Example 3 in that the antibacterial composition is adjusted to 0.5 g of lactic acid.

[0067] Comparative Example 3, a female intimate detergent, is different from Example 4 in that the antibacterial composition is adjusted to 0.5 g of lactic acid.

[0068] Comparative Example 4 is a female intimate detergent, which differs from Example 4 in that the antibacterial composition is adjusted to 0.3 g of lauric acid monoglyceride.

[0069] Comparative Example 5 is a female private detergent, which differs from Example 1 in that an equal amount of the antibacterial composition is replaced with 0.3% nano silver ions.

[0070] Comparative Example 6 is a female private detergent, which differs from Example 3 in that an equal amount of the antibacterial composition is replaced with 0.5% chlorhexidine acetate.

[0071] Comparative Example 7 is a female intimate detergent, which differs from Example 4 in that an equal amount of the antibacterial composition is replaced by 0.5% 2,4,4'-trichloro-2'-hydroxydiphenyl ether (triclosan).

[0072] Performance testing:

[0073] Antibacterial effect test: refer to QB / T 2738-2023 "Evaluation Method for Antibacterial and Antibacterial Effect of Daily Chemical Products" for strain culture and screening, suspension quantitative method for determination, and evaluation and calculation method of antibacterial rate. Specific: action time 5min, test concentration, 10% sample dilution solution, experimental bacteria, Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC 6538), Candida albicans (ATCC10231), vaginal mucus Lactobacillus (CICC6120), specific data are shown in Table 3.

[0074] Skin irritation test: Tested by zein method, specifically: weigh 5g sample, accurate to 0.001g, add water to dissolve and fix volume to obtain a test solution with an active substance content of 1%. Pipette 30mL of the test solution, add 3g of zein (Zein for short), stir at room temperature for 2h and filter, and the filtrate is used for later use. Sample blank control solution: except that Zein is not added, the other steps are the same as above. Take 5mL of the filtrate of the sample solution and the blank control solution, place them in a 250mL Kjeldahl nitrogen bottle, and add 0.2g of copper sulfate, 2.5g of potassium sulfate and 10mL of sulfuric acid in sequence. Fully digest in the digestion furnace to obtain a transparent light blue digestion solution. Digestion procedure: keep at 180℃ for 30min, heat to 260℃, keep for 30min, heat to 320℃ and keep for 30min, continue to heat to 400℃ and keep for 90min. 20 mL of sodium hydroxide solution (w=40%) was added to the digestion solution and distilled for 5 min. The generated ammonia gas was introduced into a conical flask containing 20 mL of boric acid solution (w=2%).

[0075] Sample titration: Add 10 drops of 0.1% bromocresol green-0.2% methyl red mixed indicator into a conical flask and titrate with hydrochloric acid standard solution. When the solution color changes from blue to light red, it is recorded as the titration end point, and the volume V of hydrochloric acid consumed is recorded. The nitrogen content x is calculated by the formula: Where x is the nitrogen content in the sample, mg / L; V1 is the volume of hydrochloric acid consumed by the sample solution, mL; V0 is the volume of hydrochloric acid consumed by the blank control solution, mL; C is the concentration of the hydrochloric acid standard solution, mol / L; 0.014 is the mass of nitrogen equivalent to each milliliter of hydrochloric acid standard solution, g.

[0076] The irritation values ​​in the test examples and comparative examples are shown in Table 4.

[0077] Vaginal mucosal irritation test: Examples 1 to 4 were tested and evaluated according to Section 2.3.5 of the vaginal mucosal irritation test in the Technical Specifications for Disinfection 2002. The specific experimental data are shown in Table 5.

[0078] Table 3, antibacterial test results of female private detergents of Examples 1-4 and Comparative Examples 1-4

[0079]

[0080] In Examples 1 to 4, GML and natural lactic acid are selected as antibacterial compositions. The compositions have strong antibacterial effects, and can achieve an antibacterial rate of >99% against Escherichia coli, Staphylococcus aureus, and Candida albicans. They have basically no inhibitory effect on vaginal mucus lactobacillus (CICC6120), and are even lower than those in Comparative Example 1, indicating that the solubilized GML has no destructive effect on vaginal mucus lactobacillus, and has a protective effect. Compared with Example 3 and Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 4, it is shown that GML and natural lactic acid as an antibacterial composition have an antibacterial effect that is significantly better than a single antibacterial agent, and the two have a synergistic effect.

[0081] The test results of Examples 1 to 4 and Comparative Examples 5 to 7 show that the use of conventional antibacterial agents such as chlorhexidine acetate, nano-silver ions and triclosan lotions has an antibacterial effect on Escherichia coli, Staphylococcus aureus and Candida albicans, while also having an antibacterial effect on vaginal mucus Lactobacillus, which is not conducive to the ecological balance of the flora in the body.

[0082] Table 4, Irritation test results of female private detergents of Examples 1 to 4 and Comparative Examples 2 to 7

[0083] Test Name Example 1 Example 2 Example 3 Example 4 Comparative Example 2 Irritation(mg / L) 82 85 86 90 87 Test Name Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Irritation(mg / L) 92 89 92 98 95

[0084] According to the experimental data, the irritation of comparative examples 5 to 7 is higher than that of embodiments 2 to 4, which further illustrates that the antibacterial composition with the addition of monolaurin and natural lactic acid is less irritating than the antibacterial agent with the addition of chlorhexidine acetate, nano silver ions and triclosan.

[0085] Compared with comparative examples 2-7 and embodiments 1 to 4, the irritation of embodiment 1 is low, so the combination of AES, CAB and CMEA of the surfactants in embodiment 1 has a better compounding effect.

[0086] Table 5. Vaginal mucosal irritation test results of female private detergents of Examples 1 to 4

[0087] name Example 1 Example 2 Example 3 Example 4 Vaginal mucosal irritation index 1～＜5 1～＜5 1～＜5 1～＜5 Intensity of vaginal mucosal irritation Very light Very light Very light Very light

[0088] Note: Vaginal mucosal irritation index: <1 is recorded as “no irritation”; 1 to <5 is recorded as “very mild irritation”; 5 to <9 is recorded as “mild irritation”; 9 to <12 is recorded as “moderate irritation”; ≥12 is recorded as “severe irritation”.

[0089] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An antibacterial compound, characterized in that: In parts by mass, it includes 0.3-0.5 parts of the antibacterial composition, 1 part of PEG-40 hydrogenated castor oil and 98.5-98.7 parts of water. The combined antibacterial agent includes lauric acid monoglyceride and lactic acid, and the mass ratio of lauric acid monoglyceride to lactic acid is (0.1-0.3):(0.2-0.4).

2. A female private detergent, characterized in that: In parts by mass, it includes the following raw materials: The antibacterial composition is lauric acid monoglyceride and lactic acid in a mass ratio of (0.1-0.3):(0.2-0.4).

3. A female private detergent according to claim 2, characterized in that: In parts by mass: The antibacterial composition is lauric acid monoglyceride and lactic acid in a mass ratio of (0.1-0.3):(0.2-0.4).

4. A female private part detergent according to claim 2 or 3, characterized in that: The surfactant includes one or more combinations of sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine, coconut oil monoethanolamide, amino acid surfactant, sodium lauroyl amphoacetate, and sodium cocoyl isethionate.

5. A female private detergent according to claim 2 or 3, characterized in that: The moisturizing agent includes one or a combination of glycerin, propylene glycol, hyaluronic acid, and dextran; and the chelating agent includes tetrasodium glutamate diacetate or disodium ethylenediaminetetraacetate.

6. A female private part detergent according to claim 2 or 3, characterized in that: The viscosity modifier includes one or more combinations of hydroxyethyl cellulose, carboxymethyl cellulose, xanthan gum and nonionic guar gum.

7. A female private part detergent according to claim 6, characterized in that: The viscosity of the female intimate detergent is 7000mPa·s to 10000mPa·s.

8. A female private part detergent according to claim 2 or 3, characterized in that: The pH buffering regulator includes citric acid and / or sodium citrate; the preservative includes a combination of one or more of sodium benzoate, potassium sorbate and phenoxyethanol.

9. A female private part detergent according to claim 2 or 3, characterized in that: The female private detergent has an inhibition rate of not less than 99% on Escherichia coli, Staphylococcus aureus and Candida albicans, and an inhibition rate of not more than 5% on vaginal mucus lactobacillus.

10. Use of the antibacterial compound according to claim 1 or the female intimate detergent according to any one of claims 2 to 3 in the preparation of an external vaginal care detergent for maintaining the balance of vaginal lactobacilli.

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