Fructus rosae laevigatae composite extracting solution beneficial for improving sexual function and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine extracts, and particularly discloses a fructus rosae laevigatae composite extracting solution beneficial to improving sexual functions and a preparation method thereof. The preparation method of the rosa laevigata michx compound extracting solution beneficial to improving the sexual function comprises the following steps: step 1) mixing rosa laevigata michx, dianthus superbus, yam rhizome, indigo naturalis, manyleaf paris rhizome, humifuse euphorbia herb and an ethanol water solution to obtain a fermentation substrate; the preparation method comprises the following steps of 1, preparing a fermentation substrate, 2, adding Bacillus clausii and Corynebacterium guangdongensis into the fermentation substrate, and carrying out fermentation to obtain a fermentation mixture, and 3, filtering the fermentation mixture, and carrying out sterilization to obtain the rosa laevigata michx composite extract helpful for improving the sexual function.The preparation method has the advantages that sperm quality, sperm activity and sperm quantity are well improved.

Description

Technical Field

[0001] The invention relates to the field of Chinese medicine extracts, and in particular to a Rosa laevigata composite extract liquid that helps improve sexual function and a preparation method thereof. Background Art

[0002] Sexual dysfunction is a common disease among men, mainly including sexual desire disorder, erectile dysfunction and ejaculation disorder. Sexual dysfunction can also lead to decreased sperm quality, decreased sperm activity and decreased sperm count, which can easily lead to decreased fertility and make it difficult to meet the needs of eugenics.

[0003] In order to overcome sexual dysfunction, the existing technology mainly uses kidney-yang tonifying drugs to tonify the kidney and replenish qi. Rosa rugosa is one of them, which has the effects of consolidating semen and reducing urine, consolidating uterine bleeding and stopping leukorrhea, and astringing the intestines and stopping diarrhea. However, it is found in practice that although the existing kidney-yang tonifying drugs can alleviate sexual dysfunction to a certain extent, their effects on improving sperm quality, activity, quantity, etc. are relatively general, and it is difficult to improve the pregnancy rate and meet the needs of eugenics. Therefore, there is still room for improvement. Summary of the invention

[0004] In order to better improve the quality, activity and quantity of sperm, the present application provides a composite extract of Rosa laevigata that helps to improve sexual function and a preparation method thereof.

[0005] In the first aspect, the present application provides a composite extract of Rosa laevigatae that helps to improve sexual function, using the following technical solution: A method for preparing a composite extract of Rosa laevigatae that is helpful in improving sexual function comprises the following steps: Step 1), mixing Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Dipterocarpus oleifera, Euphorbia pulex and ethanol aqueous solution to obtain a fermentation substrate; Step 2), adding Bacillus clausii and Corynebacterium cantonensis to the fermentation substrate for fermentation to obtain a fermentation mixture; Step 3), filtering and sterilizing the fermented mixture to obtain a composite extract of Rosa laevigata fruit that helps improve sexual function.

[0006] By adopting the above technical scheme, by compounding Rosa laevigata, Dianthus superbus, Herba Dioscoreae, Indigo naturalis, Herba Euphorbiae and Herba Euphorbiae and fermenting with Bacillus clausii and Corynebacterium cantonensis, the extraction efficiency of each medicinal material is high, the content of active ingredients in the extract is high, and with the mutual cooperation of the active ingredients of various medicinal materials and the fermentation products of various bacteria, it can not only play the effect of invigorating kidney yang and improving sexual dysfunction, but also regulate the body well and promote the healthy growth of sperm, so that the quality, activity and quantity of sperm are significantly improved, which helps to increase the chance of pregnancy and can better meet the needs of eugenics.

[0007] Preferably, the mass ratio of the Rosa laevigata, Dianthus superbus, Dianthus radiatae, Indigo naturalis, Dipterocarpus oleraceus and Euphorbia pulex is 20-22:4-6:3-5:2-4:6-8:3-5.

[0008] By adopting the above technical scheme and specifically selecting the mass ratio of Rosa laevigata, Dianthus superbus, Dianthus radiatae, Indigo naturalis, Dipterocarpus oleraceus and Herba Euphorbiae, the active ingredients of each Chinese medicinal material are combined in a specific ratio to exert a better synergistic effect, which can better improve the quality, activity and quantity of sperm.

[0009] Preferably, the mass ratio of the Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Dipterocarpus oleifera, Herba Euphorbiae and ethanol aqueous solution is 20-22:4-6:3-5:2-4:6-8:3-5:190-250.

[0010] By adopting the above technical scheme and specifically selecting the mass ratio of Rosa laevigata, Dianthus superbus, Herba Dioscoreae, Indigo naturalis, Herba Sophorae flavescentis, Herba Euphorbiae and ethanol aqueous solution, the efficiency of compound extraction of each medicinal material is higher, the active ingredients can be better extracted, the quality of the obtained extract is better, and the quality, activity and quantity of sperm can be better improved.

[0011] Preferably, the mass concentration of ethanol in the ethanol aqueous solution is 15-20%.

[0012] By adopting the above technical scheme, by specifically selecting the mass concentration of ethanol in the ethanol aqueous solution to be 15-20%, the extraction efficiency can be improved while the activity of each bacteria is not easily affected, so that the fermentation effect is better and the effect of the fermentation product and the active ingredient is better.

[0013] Preferably, the mass ratio of the Bacillus Clausii to the Corynebacterium cantonensis is 0.02-0.03:0.01-0.02.

[0014] By adopting the above technical scheme and specifically selecting the mass ratio of Bacillus Clausii and Corynebacterium cantonensis, the fermentation product and the active ingredient can be better coordinated, the synergistic effect can be better exerted, and the quality, activity and quantity of sperm can be better improved.

[0015] Preferably, the mass ratio of the fermentation substrate, Bacillus clausii and Corynebacterium cantonensis is 228-300:0.02-0.03:0.01-0.02.

[0016] By adopting the above technical scheme and specifically selecting the mass ratio of the fermentation substrate, Bacillus Clausii and Corynebacterium cantonensis, the fermentation can be more complete, the cost can be controlled, and the economic value is higher.

[0017] Preferably, in the step 1), the Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Dipterocarpus oleraceus and Euphorbia pulex are first chopped into particles with a particle size of ≤1 mm and then mixed with the ethanol aqueous solution.

[0018] By adopting the above technical solution, each medicinal material is easier to extract, and the prepared extract has a higher content of active ingredients and better quality.

[0019] Preferably, in step 2), the fermentation temperature is 33-37° C. and the fermentation time is 5-6 days.

[0020] By adopting the above technical scheme and specifically selecting the fermentation temperature and fermentation time, the fermentation degree can be just right, which can avoid the situation where excessive fermentation leads to too low an active ingredient content, so that the active ingredient and the fermentation product have a better effect of improving the quality, activity and quantity of sperm.

[0021] In a second aspect, the present application provides a composite extract of Rosa laevigatae that helps improve sexual function, using the following technical solution: A Rosa laevigata compound extract liquid that helps improve sexual function is prepared by the preparation method of the Rosa laevigata compound extract liquid that helps improve sexual function.

[0022] By adopting the above technical scheme, the prepared Rosa laevigata compound extract that helps to improve sexual function can relieve sexual dysfunction, nourish the kidney and replenish qi, and can also regulate the body to promote the healthy growth of sperm, and significantly improve the quality, activity and quantity of sperm.

[0023] In summary, this application has the following beneficial effects: 1. Since the present application is compounded with Rosa laevigata, Dianthus superbus, Herba Dioscoreae, Indigo naturalis, Herba Euphorbiae, and Herba Euphorbiae and fermented with Bacillus clausii and Corynebacterium cantonensis, the extraction efficiency of each medicinal material is high, the content of active ingredients in the extract is high, and the active ingredients of various medicinal materials cooperate with the fermentation products of various bacteria, which can not only replenish kidney yang and improve sexual dysfunction, but also regulate the body well and promote the healthy growth of sperm, so that the quality, activity and quantity of sperm are significantly improved, which helps to increase the chance of pregnancy and can better meet the needs of eugenics.

[0024] 2. In the present application, it is preferred to specifically select the mass ratio of Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Dipterocarpus solani and Herba Euphorbiae so that the active ingredients of each Chinese medicinal material are matched in a specific ratio to exert a better synergistic effect, which can better improve the quality, activity and quantity of sperm.

[0025] 3. In the present application, it is preferred to specifically select the mass ratio of Bacillus Clausii and Corynebacterium cantonensis so that the fermentation product and the active ingredient can be better coordinated, the synergistic effect can be better exerted, and the quality, activity and quantity of sperm can be better improved. DETAILED DESCRIPTION

[0026] The present application is further described in detail below in conjunction with embodiments.

[0027] Example 1 A method for preparing a composite extract of Rosa laevigatae that is helpful in improving sexual function comprises the following steps: Step 1), chop the Rosa laevigata, Dianthus superbus, Herba Dianthus, Herba Indigo Naturalis, Herba Dianthus and Herba Euphorbiae into particles with a particle size of ≤1 mm, respectively, and then put 20 kg of Rosa laevigata particles, 4 kg of Dianthus superbus particles, 3 kg of Herba Dianthus superbus particles, 2 kg of Herba Indigo Naturalis particles, 6 kg of Herba Dianthus superbus particles, 3 kg of Herba Euphorbiae particles and 190 kg of 15% ethanol aqueous solution into a fermentation tank, rotate at 20 r / min, stir for 5 hours, mix evenly, and obtain a fermentation substrate.

[0028] Step 2), add 0.02kg of Bacillus clausii and 0.01kg of Corynebacterium cantonense to the fermentation substrate, rotate at 5r / min, keep stirring, continuously introduce sterile air at a rate of 1L / h, keep a constant temperature of 33°C, and ferment for 5 days to obtain a fermentation mixture.

[0029] Step 3), filtering the fermentation mixture twice through an 80-mesh filter cloth, taking the filtrate, and filtering the filtrate through a 0.22 μm filter membrane for sterilization to obtain a composite extract of Rosa laevigata that helps improve sexual function.

[0030] Rosa canina comes from commercially available, sun-dried Chinese medicinal materials.

[0031] Superbus comes from commercially available, sun-dried Chinese medicinal materials.

[0032] Radix Dioscoreae comes from commercially available, sun-dried Chinese medicinal materials.

[0033] Indigo comes from commercially available, sun-dried Chinese medicinal materials.

[0034] Fleas comes from commercially available, sun-dried Chinese medicinal materials.

[0035] The herb comes from commercially available, sun-dried Chinese medicinal materials.

[0036] Bacillus clausii was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D24598.

[0037] Corynebacterium cantonense was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D50694.

[0038] Example 2 A method for preparing a composite extract of Rosa laevigatae that is helpful in improving sexual function comprises the following steps: Step 1), chop the Rosa laevigata, Dianthus superbus, Herba Dianthus, Herba Indigo Naturalis, Herba Dianthus and Herba Euphorbiae into particles with a particle size of ≤1 mm, respectively, and then put 21 kg of Rosa laevigata particles, 5 kg of Dianthus superbus particles, 4 kg of Herba Dianthus superbus particles, 3 kg of Herba Indigo Naturalis particles, 7 kg of Herba Dianthus superbus particles, 4 kg of Herba Euphorbiae particles and 220 kg of 18% ethanol aqueous solution into a fermentation tank, rotate at 20 r / min, stir for 5.5 h, mix evenly, and obtain a fermentation substrate.

[0039] Step 2), add 0.025kg of Bacillus clausii and 0.015kg of Corynebacterium cantonense to the fermentation substrate, rotate at 5r / min, keep stirring, continuously introduce sterile air at a rate of 1L / h, keep a constant temperature of 35°C, and ferment for 5.5 days to obtain a fermentation mixture.

[0040] Step 3), filtering the fermentation mixture twice through an 80-mesh filter cloth, taking the filtrate, and filtering the filtrate through a 0.22 μm filter membrane for sterilization to obtain a composite extract of Rosa laevigata that helps improve sexual function.

[0041] Rosa canina comes from commercially available, sun-dried Chinese medicinal materials.

[0042] Superbus comes from commercially available, sun-dried Chinese medicinal materials.

[0043] Radix Dioscoreae comes from commercially available, sun-dried Chinese medicinal materials.

[0044] Indigo comes from commercially available, sun-dried Chinese medicinal materials.

[0045] Fleas comes from commercially available, sun-dried Chinese medicinal materials.

[0046] The herb comes from commercially available, sun-dried Chinese medicinal materials.

[0047] Bacillus clausii was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D24598.

[0048] Corynebacterium cantonense was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D50694.

[0049] Example 3 A method for preparing a composite extract of Rosa laevigatae that is helpful in improving sexual function comprises the following steps: Step 1), chop the Rosa laevigata, Dianthus superbus, Herba Dianthus, Indigo naturalis, Herba Dianthus, and Herba Dianthus into particles with a particle size of ≤1 mm, respectively, and then put 22 kg of Rosa laevigata particles, 6 kg of Dianthus superbus particles, 5 kg of Herba Dianthus granules, 4 kg of Indigo naturalis particles, 8 kg of Herba Dianthus granules, 5 kg of Herba Dianthus granules, and 250 kg of 20% ethanol aqueous solution into a fermentation tank, rotate at 20 r / min, stir for 6 hours, mix evenly, and obtain a fermentation substrate.

[0050] Step 2), add 0.03 kg of Bacillus clausii and 0.02 kg of Corynebacterium cantonense to the fermentation substrate, rotate at 5 r / min, keep stirring, continuously introduce sterile air at a rate of 1 L / h, keep a constant temperature of 37° C., and ferment for 6 days to obtain a fermentation mixture.

[0051] Step 3), filtering the fermentation mixture twice through an 80-mesh filter cloth, taking the filtrate, and filtering the filtrate through a 0.22 μm filter membrane for sterilization to obtain a composite extract of Rosa laevigata that helps improve sexual function.

[0052] Rosa canina comes from commercially available, sun-dried Chinese medicinal materials.

[0053] Superbus comes from commercially available, sun-dried Chinese medicinal materials.

[0054] Radix Dioscoreae comes from commercially available, sun-dried Chinese medicinal materials.

[0055] Indigo comes from commercially available, sun-dried Chinese medicinal materials.

[0056] Fleas comes from commercially available, sun-dried Chinese medicinal materials.

[0057] The herb comes from commercially available, sun-dried Chinese medicinal materials.

[0058] Bacillus clausii was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D24598.

[0059] Corynebacterium cantonense was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D50694.

[0060] Comparative Example 1 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: An equal amount of Curculigo was used to replace Superbus Dianthus.

[0061] Curculigo comes from commercially available, sun-dried Chinese medicinal materials.

[0062] Comparative Example 2 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Use Cuscuta australis to replace Radix Dioscoreae with an equal amount.

[0063] Cuscuta australis comes from commercially available, sun-dried Chinese medicinal materials.

[0064] Comparative Example 3 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Replace Indigo with an equal amount of Dipsacus asper.

[0065] Dipsacus asper comes from commercially available, sun-dried Chinese medicinal materials.

[0066] Comparative Example 4 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Use an equal amount of Morinda officinalis to replace fleabane.

[0067] Morinda officinalis comes from commercially available, sun-dried Chinese medicinal materials.

[0068] Comparative Example 5 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Use an equal amount of Eucommia ulmoides to replace Herba Dijinzi.

[0069] Eucommia ulmoides comes from commercially available, sun-dried Chinese medicinal materials.

[0070] Comparative Example 6 A method for preparing a composite extract of Rosa laevigata fruit that helps improve sexual function, compared with Example 2, the only difference is: using an equal amount of Curculigo to replace Dianthus superbus, using an equal amount of Cuscuta australis to replace Dianthus sibiricum, using an equal amount of Dipsacus asper to replace Indigo naturalis, using an equal amount of Morinda officinalis to replace Paridis alba, and using an equal amount of Eucommia ulmoides to replace Herba Euphorbiae.

[0071] Curculigo comes from commercially available, sun-dried Chinese medicinal materials.

[0072] Cuscuta australis comes from commercially available, sun-dried Chinese medicinal materials.

[0073] Dipsacus asper comes from commercially available, sun-dried Chinese medicinal materials.

[0074] Morinda officinalis comes from commercially available, sun-dried Chinese medicinal materials.

[0075] Eucommia ulmoides comes from commercially available, sun-dried Chinese medicinal materials.

[0076] Comparative Example 7 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Bacillus subtilis was used to replace Bacillus clausii in equal amounts.

[0077] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D50150.

[0078] Comparative Example 8 A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, compared with Example 2, the only difference is that: Kluyveromyces marxianus was used to replace Corynebacterium cantonensis in equal amounts.

[0079] Kluyveromyces marxianus was purchased from Shanghai Collection Biotechnology Center with the number: SHBCC D13658.

[0080] Comparative Example 9 A method for preparing a composite extract of Rosa laevigata fruit that helps improve sexual function, compared with Example 2, the only difference is that Bacillus subtilis is used to replace Bacillus clausii in equal amounts, and Kluyveromyces marxianus is used to replace Corynebacterium cantonensis in equal amounts.

[0081] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center with the number: SHBCC D50150.

[0082] Kluyveromyces marxianus was purchased from Shanghai Collection Biotechnology Center with the number: SHBCC D13658.

[0083] Experiment 1 The experimental animals were 6-week-old male Kunming mice weighing 30-35 g, purchased from the Guangdong Experimental Animal Center. The mice were housed in separate cages and provided with standard feed and water, and free access to food. The room temperature was maintained at 25±1°C, with 12 h of light and 12 h of darkness per day. The handling of mice complied with the National Laboratory Animal Standards (GB14925-2001).

[0084] Sample to be tested: the composite extract of Rosa laevigatae that helps to improve sexual function in each embodiment and comparative example.

[0085] The mice were randomly divided into 11 groups, 10 mice in each group, and blank control group and experimental group were set up. The blank control group was gavaged with PBS (pH 7.4), and each experimental group was gavaged with the test sample once a day for 6 weeks.

[0086] The oral dose was 5 mg / kg bw.

[0087] Determination of sperm count, motility and deformity rate: One side of the epididymis was removed, placed in an EP tube containing 1 mL of 0.90% saline, cut into pieces, and then incubated in a 37°C constant temperature incubator for 5 minutes to allow the sperm to fully free. The semen suspension was prepared into slides, examined under a microscope, and 200 sperms were counted continuously. The number of active and inactive sperms was counted separately, and the sperm motility rate was calculated. The sperm motility rate calculation formula is: Sperm motility (%) = number of active sperm / (number of active sperm + number of immotile sperm) × 100% Take the semen suspension and prepare another slide. After eosin staining, examine under a microscope. Observe 200 sperms, count the number of deformed and non-deformed sperms, and calculate the sperm deformity rate. The calculation formula for sperm deformity rate is: Sperm deformity rate (%) = number of deformed sperm / (number of deformed sperm + number of normal sperm) × 100% Take 10uL of semen suspension and drop it into the blood cell counting plate, count the number of sperm in 5 squares (N), and determine the number of sperm. The formula for calculating the number of sperm is: number of sperm per milliliter = N × 5 × sperm dilution times × 10 4 .

[0088] The specific test data of Experiment 1 are shown in Table 1.

[0089] Table 1 According to the data comparison of each embodiment and comparative example in Table 1, the number of sperm in each embodiment is significantly higher than that in comparative examples 1-6, the sperm deformity rate is significantly lower than that in comparative examples 1-6, and the sperm motility rate is significantly higher than that in comparative examples 1-6. It can be seen that the special combination of Rosa laevigata, Herba Dianthus, Herba Dianthus, Herba Indigo, Herba Sophorae Fructus and Herba Euphorbiae can exert a synergistic effect through the active ingredients in each medicinal material, can specifically promote the healthy growth of sperm, and has a better effect of improving the quality, quantity and activity of sperm.

[0090] The sperm number of Comparative Examples 1-6 is higher than that of the blank control group, but significantly lower than that of the embodiments, the sperm deformity rate is lower than that of the blank control group, but significantly higher than that of the embodiments, and the sperm motility rate is higher than that of the blank control group, but significantly lower than that of the embodiments. It can be seen that in Comparative Examples 1-6, the raw materials with the effect of tonifying the kidney and strengthening yang are used alone or in full to replace the superbus, radix polygoni multiflori, indigo naturalis, fleabane, and herba euphorbiae in the embodiments. Although it can play the role of tonifying the kidney and replenishing qi, the effect of targeted promotion of healthy sperm growth is relatively general.

[0091] The sperm count of Comparative Examples 7-9 is higher than that of Comparative Examples 1-6, but lower than that of each embodiment, the sperm deformity rate is lower than that of Comparative Examples 1-6, but higher than that of each embodiment, and the sperm motility rate is higher than that of Comparative Examples 1-6, but lower than that of each embodiment. It can be seen that the fermentation effect is better when a combination of Bacillus Clausii and Corynebacterium cantonensis is used, and the active ingredients can be more easily absorbed. Moreover, the special fermentation products can cooperate with the active ingredients to a certain extent, thereby achieving a better effect of promoting the healthy growth of sperm.

[0092] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.

Claims

1. A method for preparing a composite extract of Rosa laevigatae that helps improve sexual function, characterized in that: The following steps are involved: Step 1), mixing Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Folium sphaerocephalae, Herba Euphorbiae and ethanol aqueous solution to obtain a fermentation substrate; Step 2), adding Bacillus clausii and Corynebacterium cantonensis to the fermentation substrate for fermentation to obtain a fermentation mixture; Step 3), filtering and sterilizing the fermented mixture to obtain a composite extract of Rosa laevigata fruit that helps improve sexual function.

2. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 1, characterized in that: The mass ratio of the Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Folium sphaerocephalae and Herba Euphorbiae is 20-22:4-6:3-5:2-4:6-8:3-5.

3. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 2, characterized in that: The mass ratio of the Rosa laevigata fruit, Dianthus superbus, Dianthus radix, Indigo naturalis, Folium sphaerocephalae, Herba Euphorbiae and ethanol aqueous solution is 20-22:4-6:3-5:2-4:6-8:3-5:190-250.

4. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 3, characterized in that: The mass concentration of ethanol in the ethanol aqueous solution is 15-20%.

5. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 4, characterized in that: The mass ratio of the Bacillus Clausii to the Corynebacterium cantoniensis is 0.02-0.03:0.01-0.

02.

6. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 5, characterized in that: The mass ratio of the fermentation substrate, Bacillus clausii and Corynebacterium cantonense is 228-300:0.02-0.03:0.01-0.

02.

7. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 1, characterized in that: In the step 1), the Rosa laevigata, Dianthus superbus, Dianthus radix, Indigo naturalis, Dipterocarpus oleraceus and Euphorbia pulex are first chopped into particles with a particle size of ≤1 mm and then mixed with the ethanol aqueous solution.

8. The method for preparing a composite extract of Rosa laevigatae that helps improve sexual function according to claim 1, characterized in that: In the step 2), the fermentation temperature is 33-37° C. and the fermentation time is 5-6 days.

9. A composite extract of Rosa laevigatae that helps improve sexual function, characterized in that: The invention is prepared by the preparation method of the composite extract of Rosa laevigata fruit that helps to improve sexual function as described in any one of claims 1 to 8.

Images