Device and process for simultaneously filtering, clarifying, concentrating and replacing cat parvovirus stock solution

Abstract

The invention discloses a device and a process for simultaneously filtering, clarifying, concentrating and replacing a cat parvovirus stock solution, relates to the technical field of cat parvovirus vaccine preparation, and solves the technical problems that the traditional concentration needs separate treatment, the exposure time is long, the virus titer influence is large, and the concentration effect is influenced. A first peristaltic pump and a second peristaltic pump are arranged on the two sides of the concentrated solution bottle respectively, a filtering mechanism is arranged at the front end of the first peristaltic pump, a virus stock solution bottle is arranged at the front end of the filtering mechanism, a replacement solution bottle is arranged on the other side of the second peristaltic pump, a third peristaltic pump is arranged at the rear end of the concentrated solution bottle, and a liquid storage tank is arranged at the front end of the third peristaltic pump. A concentration mechanism is arranged on one side of the peristaltic pump III. According to the device, all the steps are integrated together and are carried out at the same time, so that the time can be shortened, the quantity of concentrated virus liquid can be greatly increased, intermediate steps are reduced, the contact between the virus liquid and the external environment is reduced, the pollution probability is reduced, and the concentration effect is improved.

Description

Technical Field

[0001] The invention relates to the technical field of feline parvovirus vaccine preparation, and in particular to a device and a process for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution. Background Art

[0002] Feline infectious rhinotracheitis, feline infectious rhinoconjunctivitis, and feline panleukopenia (feline parvovirus) are the three most common and important viral infectious diseases in pet clinics, which seriously threaten the health of pet cats, especially young cats, and even cause the death of young cats. Therefore, it is urgent to develop vaccines for feline infectious rhinotracheitis, feline infectious rhinoconjunctivitis, and feline panleukopenia for the prevention and control of the three infectious diseases, which is of great significance to the development of my country's pet industry.

[0003] Among the three vaccines, feline parvovirus, also known as feline panleukopenia virus (FPV), is a virus that is more difficult to reproduce. (FPV infection is an acute, highly contacted, fatal intestinal infectious disease of cats, with panleukopenia, biphasic fever, vomiting, diarrhea, and hemorrhagic enteritis as the main clinical symptoms. It can also cause myocarditis and encephalitis in newborn kittens. It is one of the infectious diseases with a high mortality rate.) Its potency directly determines the immune effect and protection rate of the vaccine, so it is particularly important to improve its potency.

[0004] In order to increase the viral titer of feline panleukopenia virus and provide better quality virus stock solution for finished vaccines to improve the protective power of vaccines, it is generally carried out through centrifugal filtration concentration and replacement. This method can increase the antigen concentration in the vaccine production process, remove non-antigen impurities, ensure the safety and effectiveness of the vaccine, and reduce the side effects of vaccination and the immune interference of impurities. Due to the presence of cell fragments, serum and other flocculent precipitation in the stock solution, it will affect the use of membrane packages during the concentration process and affect the concentration process. The finished vaccine of this product is required to be a light yellowish white or light white suspension. After long-term storage, the upper layer is a clear liquid and the lower layer has a small amount of milky white precipitate. After shaking, the precipitate can be evenly dispersed to form a uniform suspension. However, after the virus liquid is concentrated, the color of the liquid will deepen due to the high concentration. During the vaccine production process, the vaccine will appear pink or dark yellow, which is inconsistent with the property requirements of the vaccine. The physical properties will change after vaccine production, so it needs to be clarified by centrifugation before concentration, and after concentration, it needs to undergo a liquid replacement process to meet the requirements of the vaccine. The traditional concentration method requires separate processing, and each process takes a certain amount of time. The longer the exposure time, the greater the impact on the virus titer, which has a certain impact on the concentration effect. Summary of the invention

[0005] The object of the present invention is to provide a device and a process for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution to solve the following technical problems:

[0006] Before traditional concentration, the virus stock solution must be centrifuged first, and then filtered to remove large particle impurities and flocculent precipitates in the virus stock solution. This process takes a certain amount of time. The more virus liquid there is, the longer it takes. The longer the virus is exposed to the outside, the greater the impact on the virus titer. At the same time, if the finished vaccine product has requirements on physical properties (because the color of the liquid deepens after concentration, it affects the color of the finished seedling), the concentrated virus stock solution needs to be replaced so that the concentrated virus liquid meets the requirements for the physical properties of the vaccine after seedling preparation. This also takes a certain amount of time. If this process takes too long, it will also have an irreversible effect on the titer of the virus liquid, thereby affecting the concentration effect.

[0007] The object of the present invention can be achieved by the following technical scheme: a device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution, comprising a concentrate bottle, a peristaltic pump 1 is arranged on one side of the concentrate bottle, a filtering mechanism is arranged at the front end of the peristaltic pump 1, a virus stock solution bottle is arranged at the front end of the filtering mechanism, a peristaltic pump 2 is arranged on the other side of the concentrate bottle, a replacement liquid bottle is arranged on the other side of the peristaltic pump 2, a peristaltic pump 3 is arranged on the rear end of the concentrate bottle, and a concentrating mechanism is arranged on one side of the peristaltic pump 3;

[0008] The concentrating mechanism includes a bottom plate, the four corners of the bottom end of the bottom plate are fixedly connected with bottom feet, a fixed clamping member is arranged on one side of the top end of the bottom plate, and two threaded rods are arranged on the other side of the fixed clamping member, the outer wall of the threaded rod is sleeved with a concentrating membrane package, the side of the concentrating membrane package is fitted with a partition, the side of the partition is provided with a rear end plate, the outer walls of the two threaded rods are sleeved with sleeves, the outer walls of the threaded rods are threadedly connected with clamping nuts, the concentrating membrane package, the partition and the rear end plate are all sleeved on the outer walls of the two threaded rods, the side of the sleeve is fitted with the side of the rear end plate, and the side of the clamping nut is fitted with the other side of the sleeve.

[0009] As a further solution of the present invention, the fixed clamping component includes a front end plate, a liquid inlet is opened at the bottom end of one side of the front end plate, a liquid outlet is opened at the bottom end of one side of the front end plate, a pressure gauge 1 is arranged on the side of the liquid inlet, a pressure gauge 2 is arranged on the side of the liquid outlet, a control valve is arranged on the other side of the pressure gauge 2, and hose connectors are arranged on the sides of the pressure gauge 1 and the control valve, a slot plate is fixedly connected to the other side of the front end plate, the front end plate is fixedly connected to the top end of the bottom plate, the front end plate is fixedly connected to the threaded rod, and the side of the concentration membrane package is clamped inside the slot plate.

[0010] As a further solution of the present invention, the clamping member includes a jacket 1, the bottom end of the jacket 1 is rotatably connected to the jacket 2 via a pin shaft, the top inner side of the jacket 2 is fixedly connected to a shaft rod, the outer wall of the shaft rod is rotatably connected to the screw rod 2, the outer wall of the screw rod 2 is threadedly connected to a fastening nut, the back of the fastening nut is fitted to the top front end of the jacket 1, and the jackets 1 and 2 are arranged on the outer walls of the connection between the liquid inlet, the pressure gauge 1, the liquid outlet, the pressure gauge 2, the control valve and the hose connector.

[0011] As a further solution of the present invention, the inner sides of the jacket 1 and the jacket 2 are both processed with semi-arc rubber sealing pads, the top of the jacket 1 is provided with a groove, and the front end of the outer wall of the screw rod 1 is embedded in the groove.

[0012] As a further scheme of the present invention, the concentrated liquid bottle includes a bottle body, and the top of the bottle body is sequentially provided with a replacement liquid inlet, a filtrate inlet, a concentrated liquid outlet and a concentrated liquid inlet, the filtrate inlet is connected to the filtration mechanism through a peristaltic pump 1 and a connecting hose, the replacement liquid inlet is connected to the replacement liquid bottle through a peristaltic pump 2 and a connecting hose, the concentrated liquid outlet is connected to a hose connector connected to the liquid inlet in the concentrating mechanism through a peristaltic pump 3 and a connecting hose, the concentrated liquid inlet is connected to a hose connector connected to the liquid outlet in the concentrating mechanism through a connecting hose, and the outer walls of the connection between the virus stock solution bottle and the connecting hose and the connection between the replacement liquid bottle and the connecting hose are both provided with a clamping mechanism.

[0013] As a further solution of the present invention, ventilation holes are provided on one side of the top of the outer wall of the concentrate bottle, virus stock solution bottle and replacement solution bottle, and an air filter element is added inside each ventilation hole.

[0014] As a further scheme of the present invention, the filtering mechanism includes a base, a bracket is fixedly connected to the top of the base, two clamps are threadedly connected to the inner side of the bracket by bolts, two clamping blocks 1 are fixedly connected to the inner rear end of the clamp, a screw rod 1 is threadedly connected to the inner side of the front end of the clamp, a clamping block 2 is rotatably connected to the back of the screw rod 1, and the inner sides of the clamping blocks 1 and 2 clamp a filter barrel, a filtrate outlet is provided at the top of the outer wall of the filter barrel, a virus stock liquid inlet is provided at the bottom of the outer wall of the filter barrel, the filtrate outlet is connected to the filtrate inlet in the concentrate bottle through a connecting hose, and the virus stock liquid inlet is connected to the virus stock liquid bottle through a connecting hose.

[0015] As a further solution of the present invention, the clamping mechanism includes a buckle 1, a side surface of the buckle 1 is rotatably connected to a buckle 2 via a pin shaft, the inner front ends of the buckle 1 and the buckle 2 are fixedly connected with a spring rod, the front end of the buckle 1 is rotatably connected to a rotating plate via a pin shaft, the other end of the rotating plate is fixedly connected with a hook, the buckle 1 and the buckle 2 are sleeved on the outer wall of the connection between the connecting hose and the virus stock solution bottle or the replacement fluid bottle, and the hook is clamped inside the front end of the buckle 2.

[0016] As a further solution of the present invention, the inner rear ends of the buckle one and the buckle two are evenly processed with a number of clamping strips, the spring rod is processed as a whole into an arc shape, and consists of a semi-arc groove tube, two semi-arc plug rods and an arc spring, the two semi-arc plug rods are slidably connected to the inside of the semi-arc groove tube, and the outer sides of the two semi-arc plug rods are respectively fixedly connected to the inner front end of the buckle one and the inner front end of the buckle two, and the arc spring is sleeved on the outer walls of the semi-arc groove tube and the two semi-arc plug rods.

[0017] The present invention also provides a process for simultaneously filtering, clarifying, concentrating and replacing a feline parvovirus stock solution, comprising the following steps:

[0018] Step 1: first add the centrifuged virus stock solution into the virus stock solution bottle, then connect the virus stock solution bottle to the virus stock solution inlet at the bottom of the filter barrel in the filtration mechanism through a connecting hose, and then connect the two ends of the connecting hose installed with the peristaltic pump 1 to the filtrate outlet at the top of the filter barrel and the filtrate inlet at the top of the bottle body in the concentrate bottle, respectively. The virus stock solution bottle is connected to the concentrate bottle through the peristaltic pump 1 and the filtration mechanism, and the centrifuged virus stock solution is filtered and flows into the concentrate bottle. Multiple bottles of virus stock solution can be prepared. After one bottle of virus stock solution is filtered, the virus stock solution bottle is replaced by the clamping mechanism to continue the liquid replenishment;

[0019] Step 2: After the concentrate bottle and the virus stock solution bottle are connected through the peristaltic pump 1 and the filtering mechanism, the concentrate outlet opened at the top of the bottle body in the concentrate bottle is connected to the hose connector connected to the liquid inlet in the concentrating mechanism through the connecting hose and the peristaltic pump 3, and the concentrate inlet opened at the top of the bottle body in the concentrate bottle is connected to the hose connector connected to the liquid outlet in the concentrating mechanism through another connecting hose. The liquid in the concentrate bottle is connected to the concentrating membrane package through the peristaltic pump 3, so as to concentrate the filtered virus stock solution;

[0020] Step three, while filtering and concentrating the virus stock solution, connect the replacement fluid inlet opened at the top of the bottle body in the concentrate bottle to the replacement fluid bottle through a connecting hose and peristaltic pump 2. During the concentration process, the concentrate bottle and the replacement fluid bottle are connected through peristaltic pump 2, so that the concentrated liquid is replaced at the same time. Similar to step one, multiple bottles of replacement fluid can be prepared. After the replacement of the concentrated liquid in one bottle of replacement fluid and the concentrated liquid in the concentrate bottle is completed, the concentrate bottle is replaced by a clamping mechanism for fluid replenishment.

[0021] Beneficial effects of the present invention:

[0022] (1) Through the process of replacing the concentrated liquid of the present invention, after the parvovirus is concentrated, the color of the liquid will be darker due to the high concentration. After the seedling is completed, the color requirements of the physical properties of the finished vaccine will be different, resulting in unqualified physical properties of the finished product (the finished product of this vaccine is required to be a light yellowish white or light white suspension. After long-term storage, the upper layer is a clear liquid and the lower layer has a small amount of milky white precipitate. After shaking, the precipitate can be evenly dispersed to form a uniform suspension. However, after the virus liquid is concentrated, the color of the liquid will be darker due to the high concentration. During the seedling process, the vaccine will appear pink or dark yellow, which does not meet the property requirements of the vaccine). Therefore, it is necessary to replace the culture medium in the virus concentrate before seedling. This process replaces the culture medium in the virus concentrate with the PBS solution through membrane packaging without losing the potency of the concentrate, and replaces the concentrate from dark pink or dark yellow to light yellow or colorless liquid, and does not lose the replaced virus, thereby meeting the quality and property requirements of the finished vaccine;

[0023] (2) This device integrates the three steps of clarification before concentration, concentration and replacement after concentration into a complete closed-loop process through a series of operations and devices, reducing the chances of the virus liquid coming into contact with the outside world at each intermediate link and reducing the chance of contamination. At the same time, it greatly shortens the operation process, saves the exposure time of the virus liquid, and reduces the rate of loss of potency, thereby improving the concentration effect and meeting the concentration requirements;

[0024] (3) The process of this device integrates all the steps together, and concentrates and replaces the virus stock liquid at the same time as the impurity removal and filtration, thereby reducing the overall concentration time, thereby reducing the virus exposure time and reducing the impact on the virus titer. The simultaneous implementation of several steps can not only shorten the time, but also greatly increase the amount of concentrated virus liquid, reduce the tedious intermediate steps, reduce the contact between the virus liquid and the external environment, and reduce the chance of contamination. This method can increase the antigen concentration in the vaccine production process, remove non-antigen impurities, ensure the safety and effectiveness of the vaccine, reduce the side effects of vaccination and the immune interference of impurities, and improve the concentration effect overall. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1It is a schematic diagram of the connection structure of the device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution of the present invention;

[0026] Figure 2 The present invention Figure 1 Schematic diagram of the connection structure of the concentrate bottle;

[0027] Figure 3 The present invention Figure 1 Schematic diagram of the connection structure of the concentrate bottle, peristaltic pump and concentration mechanism;

[0028] Figure 4 The present invention Figure 1 Schematic diagram of the connection structure of the concentrate bottle, peristaltic pump 1, filtration mechanism and virus stock solution bottle;

[0029] Figure 5 The present invention Figure 4 Schematic diagram of the connection structure of the filtering mechanism;

[0030] Figure 6 The present invention Figure 1 Schematic diagram of the connection structure of the clamping mechanism;

[0031] Figure 7 The present invention Figure 3 Schematic diagram of the connection structure of the intermediate concentration mechanism;

[0032] Figure 8 The present invention Figure 7 Structural disassembly diagram of the fixed and clamping components;

[0033] Fig. 9 The present invention Figure 7 Another schematic diagram of the axonometric connection structure of the fixed pressing member and the threaded rod;

[0034] Fig.10 The present invention Figure 7 Another isometric connection structure schematic diagram;

[0035] Fig.11 The present invention Figure 7 Schematic diagram of the connection structure of the clamping member.

[0036] In the figure: 1. concentrate bottle; 101. bottle body; 102. replacement liquid inlet; 103. filtrate inlet; 104. concentrate outlet; 105. concentrate inlet; 2. peristaltic pump 1; 3. filter mechanism; 301. base; 302. bracket; 303. clamp; 304. clamp block 1; 305. screw rod 1; 306. clamp block 2; 307. filter barrel; 308. filtrate outlet; 309. virus stock liquid inlet; 4. virus stock liquid bottle; 5. peristaltic pump 2; 6. replacement liquid bottle; 7. peristaltic pump 3; 8. concentration mechanism; 801. bottom plate; 802. foot; 803. fixed pressing member; 8031. front end plate ;8032, liquid inlet;8033, liquid outlet;8034, pressure gauge one;8035, pressure gauge two;8036, control valve;8037, hose connector;8038, slot plate;804, clamping member;8041, jacket one;8042, jacket two;8043, shaft rod;8044, screw rod two;8045, fastening nut;805, threaded rod;806, concentration membrane package;807, partition;808, rear end plate;809, sleeve;810, pressing nut;9, clamping mechanism;901, buckle one;902, buckle two;903, spring rod;904, rotating plate;905, hook. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Embodiment 1

[0039] See also Figure 1-Figure 11As shown, the present invention is a device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution, comprising a concentrate bottle 1, a peristaltic pump 2 is arranged on one side of the concentrate bottle 1, the peristaltic pump 2 is used to pump the virus stock solution in the virus stock solution bottle 4 into the filtering mechanism 3, and pump the filtered virus stock solution into the interior of the concentrate bottle 1, the front end of the peristaltic pump 2 is provided with a filtering mechanism 3, the filtering mechanism 3 is used to filter large particle impurities and flocculent precipitation in the virus stock solution, the front end of the filtering mechanism 3 is provided with a virus stock solution bottle 4, the virus stock solution bottle 4 is used to hold The virus stock solution after centrifugation is placed, and a peristaltic pump 2 5 is arranged on the other side of the concentrate bottle 1. The peristaltic pump 2 5 is used to pump the replacement fluid in the replacement fluid bottle 6 into the interior of the concentrate bottle 1. A replacement fluid bottle 6 is arranged on the other side of the peristaltic pump 2 5. The replacement fluid bottle 6 is used to hold the replacement fluid. A peristaltic pump 3 7 is arranged at the rear end of the concentrate bottle 1. The peristaltic pump 3 7 is used to pump the replacement fluid in the concentrate bottle 1 and the filtered virus stock solution into the concentration mechanism 8. A concentration mechanism 8 is arranged on one side of the peristaltic pump 3 7. The concentration mechanism 8 is used to concentrate the virus stock solution;

[0040] The concentrating mechanism 8 includes a bottom plate 801, and the four corners of the bottom end of the bottom plate 801 are fixedly connected with bottom feet 802, and the bottom feet 802 are used to support the bottom plate 801. A fixed pressing member 803 is provided on one side of the top of the bottom plate 801. The fixed pressing member 803 is used to clamp the concentrated membrane package 806 and connect the concentrated membrane package 806 with the concentrated liquid bottle 1. Two threaded rods 805 are provided on the other side of the fixed pressing member 803. The two threaded rods 805 are used to support the concentrated membrane package 806, the partition 807 and the rear end plate 808. The outer wall of the threaded rod 805 is provided with a concentrated membrane package 806, and the concentrated membrane package 806 is used to concentrate the virus stock solution. The side of the concentrated membrane package 806 is fitted with a partition 807, and the partition 807 is used to enclose the side of the concentrated membrane package 806 to avoid The virus stock solution permeates out from the side of the concentrated membrane package 806, and a rear end plate 808 is set on the side of the partition 807. The rear end plate 808 is used to compress the concentrated membrane package 806 and the partition 807. The outer walls of the two threaded rods 805 are sleeved with sleeves 809, and the outer walls of the threaded rods 805 are threadedly connected with a clamping nut 810. Through the cooperation of the sleeve 809 and the clamping nut 810, the rear end plate 808 can be pressed to one side of the partition 807, so that the connection between the fixed clamping component 803, the concentrated membrane package 806 and the partition 807 is tighter. The concentrated membrane package 806, the partition 807 and the rear end plate 808 are all sleeved on the outer walls of the two threaded rods 805, the side of the sleeve 809 is attached to the side of the rear end plate 808, and the side of the clamping nut 810 is attached to the other side of the sleeve 809.

[0041] In this embodiment, preferably, the fixed pressing member 803 includes a front end plate 8031, and a liquid inlet 8032 is provided at the bottom end of one side of the front end plate 8031. The liquid inlet 8032 is used to input the virus stock solution into the interior of the concentration membrane package 806. A liquid outlet 8033 is provided at the bottom end of one side of the front end plate 8031. The liquid outlet 8033 is used to discharge the virus stock solution concentrated by the concentration membrane package 806 from the interior. A pressure gauge 1 8034 is provided on the side of the liquid inlet 8032, and a pressure gauge 2 8035 is provided on the side of the liquid outlet 8033. The pressure gauges 1 8034 and 8035 are used to monitor the pressure inside the concentration membrane package 806 and the pressure in the pipeline. A control valve 8036 is provided on the other side of the pressure gauge 2 8035. The control valve 8036 is used to control the rate at which the concentrated virus stock solution is discharged from the concentration membrane package 806. , the sides of the pressure gauge 8034 and the control valve 8036 are both provided with hose connectors 8037, and the hose connector 8037 is used to clamp the end of the connecting hose connected thereto to prevent the connecting hose from falling off from its outer wall during operation. A slot plate 8038 is fixedly connected to the other side of the front end plate 8031, and the slot plate 8038 is used to clamp one side of the concentrated membrane package 806, so that the concentrated membrane package 806 can be easily installed on the side of the front end plate 8031. The front end plate 8031 ​​is fixedly connected to the top of the bottom plate 801, and the front end plate 8031 ​​is used to press the front end of the concentrated membrane package 806, and connect the concentrated membrane package 806 with the concentrated liquid bottle 1 through the peristaltic pump three 7 and two connecting hoses. The front end plate 8031 ​​is fixedly connected to the threaded rod 805, and the side of the concentrated membrane package 806 is clamped inside the slot plate 8038.

[0042] In this embodiment, preferably, the clamping member 804 includes a jacket 8041, and the bottom end of the jacket 8041 is rotatably connected to the jacket 8042 through a pin shaft. Through the cooperation between the jacket 8041 and the jacket 8042, the connection between the liquid inlet 8032, the pressure gauge 8034, one of the two hose connectors 8037, the liquid outlet 8033, the pressure gauge 8035, the control valve 8036 and the other of the two hose connectors 8037 can be fixed. The inner side of the top of the jacket 8042 is fixedly connected to the shaft rod 8043, and the shaft rod 8043 is used to support the screw rod 8044, so that the screw rod 8044 can be fixed with the shaft rod 8044. 3 is the axis rotation, the outer wall of the shaft 8043 is rotatably connected with the screw rod 8044, the screw rod 8044 is used to support the fastening nut 8045, the outer wall of the screw rod 8044 is threadedly connected with the fastening nut 8045, the fastening nut 8045 can lock the jacket 1 8041 and the jacket 2 8042 together through the cooperation with the screw rod 8044, the back of the fastening nut 8045 is attached to the top front end of the jacket 1 8041, and the jacket 1 8041 and the jacket 2 8042 are sleeved on the outer wall of the connection between the liquid inlet 8032, the pressure gauge 1 8034, the liquid outlet 8033, the pressure gauge 2 8035, the control valve 8036 and the hose connector 8037.

[0043] In this embodiment, preferably, the inner sides of the jacket 1 8041 and the jacket 2 8042 are processed with semi-arc rubber sealing pads, the top of the jacket 1 8041 is provided with a groove, and the front end of the outer wall of the screw rod 1 305 is embedded in the inside of the groove.

[0044] In summary, before concentrating the virus stock solution, the filter mechanism 3 needs to be assembled first. When assembling the filter mechanism 3, first fit the liquid inlet 8032 opened on one side of the front end plate 8031 ​​in the fixed pressing member 803 together with the pressure gauge 1 8034, and then let the jacket 1 8041 and the jacket 2 8042 in the clamping member 804 be sleeved to the outer wall of the connection between the liquid inlet 8032 and the pressure gauge 1 8034, and the screw rod 2 8044 is clamped to the inside of the groove opened at the top of the jacket 1 8041, and then tighten the fastening nut 8045 so that the back of the fastening nut 8045 fits to the top front end of the jacket 1 8041. Thus, the jacket 1 8041 and the jacket 2 8042 can be locked to the outer surface of the connection between the pressure gauge 1 8034 and the liquid inlet 8032, so as to fix it. Then, the above operation is repeated to sequentially lock the connection between the pressure gauge 1 8034 and one of the two hose connectors 8037, the connection between the liquid outlet 8033 and the pressure gauge 2 8035, the connection between the pressure gauge 2 8035 and the control valve 8036, and the connection between the control valve 8036 and the other of the two hose connectors 8037. The assembly of the fixed pressing member 803 is completed, and then a concentration membrane to be used is taken out. The pack 806 is clamped with one side thereof to the inside of the card slot plate 8038 fixed to the other side of the front end plate 8031, so that the concentrated membrane pack 806 can be initially fixed, and then the partition plate 807 is sleeved to the outer wall of the two threaded rods 805, and the side of the partition plate 807 is fitted to the other side of the concentrated membrane pack 806, and then the rear end plate 808 is also sleeved to the outer wall of the two threaded rods 805, so that the side of the rear end plate 808 is fitted to the other side of the partition plate 807, and then the sleeve 809 is sleeved to the outer wall of the threaded rod 805, and the pressing nut 810 is tightened to the side of the rear end plate 808, and the filter mechanism 3 can be assembled as a whole. After filtering mechanism 3, the hose connector 8037 connected to the liquid inlet 8032 is connected to the concentrated liquid outlet 104 opened at the top of the bottle body 101 in the concentrated liquid bottle 1 through the connecting hose and the peristaltic pump three 7, and then the concentrated liquid inlet 105 opened at the top of the bottle body 101 is also connected to the hose connector 8037 connected to the liquid outlet 8033 through the connecting hose. Through the operation of the peristaltic pump three 7, the filtered virus stock solution in the concentrated liquid bottle 1 can be pumped into the concentrating mechanism 8, and through the operation of the concentrating membrane package 806, the concentrated virus stock solution is pumped back into the concentrated liquid bottle 1, thereby completing the concentration of the virus stock solution.

[0045] Embodiment 2

[0046] See also Figure 1 , Figure 2 , Figure 4 and Figure 5As shown, on the basis of the first embodiment, the concentrated liquid bottle 1 includes a bottle body 101, and the top of the bottle body 101 is provided with a replacement liquid inlet 102, a filtrate inlet 103, a concentrated liquid outlet 104 and a concentrated liquid inlet 105 in sequence. The filtrate inlet 103 is connected to the filtering mechanism 3 through a peristaltic pump 1 2 and a connecting hose. After the virus stock liquid stored in the virus stock liquid bottle 4 is filtered by the filtering mechanism 3, the filtered virus stock liquid will be discharged from the filtrate inlet 103 to the bottle body 101 through the operation of the peristaltic pump 1 2. The replacement liquid inlet 102 is connected to the replacement liquid bottle 6 through a peristaltic pump 2 5 and a connecting hose. The replacement liquid stored in the replacement liquid bottle 6 will be transported from the replacement liquid inlet 102 to the inside of the concentrated liquid bottle 1 through the operation of the peristaltic pump 2 5. The concentrated liquid outlet 104 is connected to the hose connector 8037 connected to the liquid inlet 8032 in the concentrating mechanism 8 through a peristaltic pump 3 7 and a connecting hose. The concentrated liquid inlet 105 is connected to the hose connector 8037 connected to the liquid inlet 8032 in the concentrating mechanism 8 through a connecting hose. It is connected to the hose connector 8037 connected to the liquid outlet 8033 in the concentration mechanism 8. Through the cooperation of the peristaltic pump 37 and the concentration mechanism 8, the virus stock solution in the bottle body 101 can be pumped from the concentrated liquid outlet 104 to the concentrated membrane package 806, and the virus stock solution is concentrated. The concentrated virus stock solution is re-delivered to the bottle body 101 through the concentrated liquid inlet 105, thereby completing the concentration of the virus stock solution. The outer walls of the connection between the virus stock solution bottle 4 and the connecting hose and the connection between the replacement liquid bottle 6 and the connecting hose are both provided with a clamping mechanism 9. The clamping mechanism 9 is used to lock the connection between the connecting hose and the virus stock solution bottle 4 or the replacement liquid bottle 6 to prevent the connecting hose from falling off from the connection during the liquid pumping project, and can be clamped at the end connected to it when the virus stock solution bottle 4 or the replacement liquid bottle 6 needs to be replaced, thereby preventing liquid from flowing out of the connecting hose, which is convenient for replacing each virus stock solution bottle 4 or the replacement liquid bottle 6.

[0047] In the present embodiment, preferably, a vent hole is provided on one side of the top of the outer wall of the concentrate bottle 1, the virus stock solution bottle 4 and the replacement solution bottle 6 to ensure air circulation between the bottle and the outside, so that the liquid in the pipelines of each connecting hose can circulate, and an air filter element is provided inside each vent hole to filter the air entering the vent hole to prevent dust from entering the bottle along with the air and causing contamination to the liquid in the bottle.

[0048] In the present embodiment, preferably, the filter mechanism 3 includes a base 301, a bracket 302 is fixedly connected to the top of the base 301, the bracket 302 is used to support the clamp 303, the inner side of the bracket 302 is threadedly connected to two clamps 303 by bolts, the clamp 303 is used to fix the clamp block 1 304 and support the screw rod 1 305, the inner rear end of the clamp 303 is fixedly connected to two clamp blocks 1 304, the clamp block 1 304 is used to clamp the outer wall of the filter barrel 307, the front end of the clamp 303 is internally threadedly connected to the screw rod 1 305, the screw rod 1 305 is used to drive the clamp block 2 306 to move, the back of the screw rod 1 305 is rotatably connected to the clamp block 2 306, and the filter barrel 307 can be clamped and fixed on the clamp 3 by the cooperation between the clamp block 1 304 and the clamp block 2 306. 03, it is convenient to install the filter barrel 307 and replace the hollow fiber filter element in the filter barrel 307. The inner sides of the clamp block 1 304 and the clamp block 2 306 hold the filter barrel 307. The filter barrel 307 is used to filter large particles of impurities and flocculent precipitation in the virus stock solution after centrifugation. A filtrate outlet 308 is provided at the top of the outer wall of the filter barrel 307 for conveying the filtered virus stock solution to the concentrated solution bottle 1. A virus stock solution inlet 309 is provided at the bottom of the outer wall of the filter barrel 307 for conveying the centrifuged, unfiltered virus stock solution to the interior of the filter barrel 307. The filtrate outlet 308 is connected to the filtrate inlet 103 in the concentrated solution bottle 1 through a connecting hose, and the virus stock solution inlet 309 is connected to the virus stock solution bottle 4 through a connecting hose.

[0049] In summary, before concentrating the virus stock solution, the centrifuged virus stock solution needs to be filtered to remove large particle impurities and flocculent precipitation in the virus stock solution. At this time, the filter barrel 307 in the filter mechanism 3 is first placed on the inner side of the clamp 303, and then the screw rod 1 305 is twisted to drive the clamp block 2 306 to move backward through the screw rod 1 305, so that the filter barrel 307 is clamped and fixed to the inner side of the clamp 303 through the cooperation between the clamp block 1 304 and the clamp block 2 306. Then, the virus stock solution bottle 4 containing the centrifuged virus stock solution is connected to the virus stock solution inlet 309 opened at the bottom end of the outer wall of the filter barrel through a connecting tube, and the filtrate outlet 308 opened at the top end of the outer wall of the filter barrel 307 is connected to the filtrate inlet 103 opened at the top end of the bottle body 101 through a connecting hose and a peristaltic pump 2, so that the filtered virus stock solution can be transported to the concentrated liquid bottle 1 while filtering the virus stock solution, so as to facilitate the subsequent concentration of the filtered virus stock solution.

[0050] Embodiment 3

[0051] See also Figure 1 and Figure 6As shown, on the basis of the first and second embodiments, the clamping mechanism 9 includes a buckle 901, and the side of the buckle 901 is rotatably connected with a buckle 902 through a pin shaft. The buckle 901 and the buckle 902 are used to clamp the connection between the connecting hose and the virus stock solution bottle 4 or the connection between the connecting hose and the replacement fluid bottle 6 to prevent the connecting hose from falling off from the virus stock solution bottle 4 or the replacement fluid bottle 6 during the process of pumping the liquid. The inner front end of the buckle 901 and the buckle 902 is fixedly connected with a spring rod 903. The spring rod 903 is fixedly connected to the inner front end of the buckle 901 and the buckle 902. Rod 903 is used to eject buckle 1 901 and buckle 2 902. The front end of buckle 1 901 is rotatably connected to a rotating plate 904 through a pin shaft. The rotating plate 904 is used to fix a hook 905. The other end of the rotating plate 904 is fixedly connected to a hook 905. The hook 905 is used to clamp buckle 1 901 and buckle 2 902 together. Buckle 1 901 and buckle 2 902 are sleeved on the outer wall of the connection between the connecting hose and the virus stock solution bottle 4 or the replacement solution bottle 6, and the hook 905 is clamped inside the front end of buckle 2 902.

[0052] In the present embodiment, preferably, the inner rear ends of the buckle 1 901 and the buckle 2 902 are uniformly processed with a plurality of clamping strips. When the virus stock solution bottle 4 or the replacement solution bottle 6 needs to be replaced, the buckle 1 901 and the buckle 2 902 can be slid forward so that the position processed with the clamping strips is clamped to the outer wall of the connecting hose, so that the end of the connecting hose can be clamped to prevent liquid from flowing out of the connecting hose. The spring rod 903 is processed into an arc shape as a whole, and consists of a semi-arc groove tube, two semi-arc plug rods and an arc spring. The two semi-arc plug rods are slidably connected to the semi-arc groove tube. Inside, the outer sides of the two semi-arc-shaped plug rods are fixedly connected with the inner front end of buckle 1 901 and the inner front end of buckle 2 902 respectively, and the arc spring is sleeved on the outer walls of the semi-arc-shaped groove tube and the two semi-arc-shaped plug rods. After canceling the mutual clamping of the front ends of buckle 1 901 and buckle 2 902, under the action of spring rod 903, buckle 1 901 and buckle 2 902 can be opened outward, so that buckle 1 901 and buckle 2 902 can be removed from the end of the outer wall of the connecting hose, or moved forward so that the position where the clamping strip is processed is clamped to the outer wall of the end of the connecting hose.

[0053] In summary, after the concentrate bottle 1, the virus stock solution bottle 4 and the replacement fluid bottle 6 are connected, since the peristaltic pump will cause a large amount of shaking at the end of the connecting hose when it is working, at this time, the buckle 1 901 and the buckle 2 902 in the clamping mechanism 9 are set to the connection between the connecting hose and the virus stock solution bottle 4 or the replacement fluid bottle 6, and the middle sections of the buckle 1 901 and the buckle 2 902 are buckled to the outer wall of the connecting hose, and then, the buckle 1 901 and the buckle 2 902 are buckled together, and the hook 905 is snapped to the front end of the buckle 2 902, so that the buckle 1 901 and the buckle 2 902 are closed together, and the connection between the connecting hose and the virus stock solution bottle 4 or the replacement fluid bottle 6 can be clamped, thereby preventing the connecting hose from falling off from the virus stock solution bottle 4 or the replacement fluid bottle 6, and when the virus stock solution bottle 4 or the replacement fluid bottle 6 needs to be replaced, the rotating plate 904 is pulled outward, The hook 905 cancels the clamping of the buckle 2 902. Under the action of the spring rod 903, the buckle 1 901 and the buckle 2 902 can be expanded outward, and then the positions where the clamping strips are processed on the inner sides of the buckle 1 901 and the buckle 2 902 are fitted to the outer wall of the connecting hose, and keep a certain distance from the connection between the virus stock solution bottle 4 or the replacement fluid bottle 6. Then, the buckle 1 901 and the buckle 2 902 are closed again, and the hook 905 is clamped to the inner side of the front end of the buckle 2 902 through the rotating plate 904, that is, the buckle 1 901 and the buckle 2 902 are clamped together again, so that the connecting hose is clamped by the clamping strip, so that it is closed together to prevent liquid from flowing out from the inside of the connecting hose. In this way, the virus stock solution bottle 4 or the replacement fluid bottle 6 can be replaced. After the replacement is completed, the clamping mechanism 9 is clamped to the outer wall of the connection between the connecting hose and the virus stock solution bottle 4 or the replacement fluid bottle 6.

[0054] Embodiment 4

[0055] See also Figure 1-Figure 11 Combining Example 1, Example 2 and Example 3, the present embodiment is obtained, a process for simultaneously filtering, clarifying, concentrating and replacing a feline parvovirus stock solution, comprising the following steps:

[0056] Step 1, first add the centrifuged virus stock solution into the virus stock solution bottle 4, then connect the virus stock solution bottle 4 to the virus stock solution inlet 309 opened at the bottom of the filter barrel 307 in the filter mechanism 3 through the connecting hose, and then connect the two ends of the connecting hose installed with the peristaltic pump 2 to the filtrate outlet 308 opened at the top of the filter barrel 307 and the filtrate inlet 103 opened at the top of the bottle body 101 in the concentrate bottle 1, respectively, the virus stock solution bottle 4 is connected to the concentrate bottle 1 through the peristaltic pump 2 and the filter mechanism 3, and the centrifuged virus stock solution is filtered and flows into the concentrate bottle 1. Multiple bottles of virus stock solution can be prepared. After a bottle of virus stock solution is filtered, the virus stock solution bottle 4 is replaced by the clamping mechanism 9 to continue the liquid replenishment;

[0057] Step 2: After the concentrate bottle 1 and the virus stock solution bottle 4 are connected through the peristaltic pump 1 2 and the filtering mechanism 3, the concentrate outlet 104 opened at the top of the bottle body 101 in the concentrate bottle 1 is connected to the hose connector 8037 connected to the liquid inlet 8032 in the concentrating mechanism 8 through the connecting hose and the peristaltic pump 3 7, and the concentrate inlet 105 opened at the top of the bottle body 101 in the concentrate bottle 1 is connected to the hose connector 8037 connected to the liquid outlet 8033 in the concentrating mechanism 8 through another connecting hose. The liquid in the concentrate bottle 1 is connected to the concentrating membrane package 806 through the peristaltic pump 3 7, so as to concentrate the filtered virus stock solution;

[0058] Step three, while filtering and concentrating the virus stock solution, the replacement fluid inlet 102 opened at the top of the bottle body 101 in the concentrate bottle 1 is connected to the replacement fluid bottle 6 through a connecting hose and a peristaltic pump 25. During the concentration process, the concentrate bottle 1 is connected to the replacement fluid bottle 6 through the peristaltic pump 25, so that the concentrated liquid is replaced at the same time. Similar to step one, multiple bottles of replacement fluid can be prepared. After the replacement of the concentrated liquid in one bottle of replacement fluid and the concentrated liquid in the concentrate bottle 1 is completed, the concentrate bottle 1 is replaced by the clamping mechanism 9 for fluid replenishment.

[0059] Through the above steps, large quantities of feline parvovirus stock solution can be concentrated and replaced, which will greatly save time, shorten the concentration time and the pollution risks caused by frequent equipment replacement during the process, and effectively improve the concentration effect.

[0060] The above is a detailed description of an embodiment of the present invention, but the content is only a preferred embodiment of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made according to the scope of application of the present invention should still fall within the scope of application of the present invention.

Claims

1. A device for filtering, clarifying, concentrating and replacing feline parvovirus stock solution at the same time, comprising a concentrate bottle, a peristaltic pump 1 is arranged on one side of the concentrate bottle, a filtering mechanism is arranged at the front end of the peristaltic pump 1, a virus stock solution bottle is arranged at the front end of the filtering mechanism, a peristaltic pump 2 is arranged on the other side of the concentrate bottle, a replacement liquid bottle is arranged on the other side of the peristaltic pump 2, a peristaltic pump 3 is arranged at the rear end of the concentrate bottle, characterized in that: A concentrating mechanism is provided on one side of the peristaltic pump 3; The concentrating mechanism includes a bottom plate, the four corners of the bottom end of the bottom plate are fixedly connected with bottom feet, a fixed clamping member is arranged on one side of the top end of the bottom plate, and two threaded rods are arranged on the other side of the fixed clamping member, the outer wall of the threaded rod is sleeved with a concentrating membrane package, the side of the concentrating membrane package is fitted with a partition, the side of the partition is provided with a rear end plate, the outer walls of the two threaded rods are sleeved with sleeves, the outer walls of the threaded rods are threadedly connected with clamping nuts, the concentrating membrane package, the partition and the rear end plate are all sleeved on the outer walls of the two threaded rods, the side of the sleeve is fitted with the side of the rear end plate, and the side of the clamping nut is fitted with the other side of the sleeve.

2. A device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: The fixed clamping component includes a front end plate, a liquid inlet is opened at the bottom end of one side of the front end plate, a liquid outlet is opened at the bottom end of one side of the front end plate, a pressure gauge 1 is arranged on the side of the liquid inlet, a pressure gauge 2 is arranged on the side of the liquid outlet, a control valve is arranged on the other side of the pressure gauge 2, and hose connectors are arranged on the sides of the pressure gauge 1 and the control valve, a slot plate is fixedly connected to the other side of the front end plate, the front end plate is fixedly connected to the top end of the bottom plate, the front end plate is fixedly connected to the threaded rod, and the side of the concentration membrane package is clamped inside the slot plate.

3. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: The clamping member includes a jacket 1, the bottom end of the jacket 1 is rotatably connected to the jacket 2 via a pin shaft, the inner side of the top of the jacket 2 is fixedly connected to a shaft rod, the outer wall of the shaft rod is rotatably connected to the screw rod 2, the outer wall of the screw rod 2 is threadedly connected to a fastening nut, the back of the fastening nut is fitted to the top front end of the jacket 1, and the jackets 1 and 2 are arranged on the outer walls of the connection between the liquid inlet, the pressure gauge 1, the liquid outlet, the pressure gauge 2, the control valve and the hose connector.

4. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: The inner sides of the jacket 1 and the jacket 2 are both processed with semi-arc rubber sealing pads, the top of the jacket 1 is provided with a groove, and the front end of the outer wall of the screw rod 1 is embedded in the groove.

5. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: The concentrated liquid bottle comprises a bottle body, and a replacement liquid inlet, a filtrate inlet, a concentrated liquid outlet and a concentrated liquid inlet are sequentially provided at the top of the bottle body. The filtrate inlet is connected to the filtering mechanism through a peristaltic pump 1 and a connecting hose, the replacement liquid inlet is connected to the replacement liquid bottle through a peristaltic pump 2 and a connecting hose, the concentrated liquid outlet is connected to a hose connector connected to a liquid inlet in a concentrating mechanism through a peristaltic pump 3 and a connecting hose, the concentrated liquid inlet is connected to a hose connector connected to a liquid outlet in a concentrating mechanism through a connecting hose, and the outer walls of the connection between the virus stock liquid bottle and the connecting hose and the connection between the replacement liquid bottle and the connecting hose are both provided with a clamping mechanism.

6. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: A ventilation hole is provided on one side of the top end of the outer wall of the concentrate bottle, the virus stock solution bottle and the replacement solution bottle, and an air filter element is added inside each ventilation hole.

7. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 1, characterized in that: The filtering mechanism includes a base, a bracket is fixedly connected to the top of the base, two clamps are threadedly connected to the inner side of the bracket by bolts, two clamping blocks 1 are fixedly connected to the inner rear end of the clamp, a screw rod 1 is threadedly connected to the inner side of the front end of the clamp, a clamping block 2 is rotatably connected to the back of the screw rod 1, and the inner sides of the clamping blocks 1 and 2 clamp a filter barrel, a filtrate outlet is provided at the top of the outer wall of the filter barrel, a virus stock liquid inlet is provided at the bottom of the outer wall of the filter barrel, the filtrate outlet is connected to the filtrate inlet in the concentrate bottle through a connecting hose, and the virus stock liquid inlet is connected to the virus stock liquid bottle through a connecting hose.

8. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 5, characterized in that: The clamping mechanism includes a buckle 1, a side surface of the buckle 1 is rotatably connected to a buckle 2 via a pin shaft, the inner front ends of the buckle 1 and the buckle 2 are fixedly connected with a spring rod, the front end of the buckle 1 is rotatably connected to a rotating plate via a pin shaft, the other end of the rotating plate is fixedly connected with a hook, the buckle 1 and the buckle 2 are sleeved on the outer wall of the connection between the connecting hose and the virus stock solution bottle or the replacement fluid bottle, and the hook is clamped inside the front end of the buckle 2.

9. The device for simultaneously filtering, clarifying, concentrating and replacing feline parvovirus stock solution according to claim 7, characterized in that: The inner rear ends of the buckle one and the buckle two are evenly processed with a plurality of clamping strips, and the spring rod is processed as a whole into an arc shape, which consists of a semi-arc groove tube, two semi-arc plug rods and an arc spring. The two semi-arc plug rods are slidably connected to the inside of the semi-arc groove tube, and the outer sides of the two semi-arc plug rods are respectively fixedly connected to the inner front ends of the buckle one and the inner front ends of the buckle two, and the arc spring is sleeved on the outer walls of the semi-arc groove tube and the two semi-arc plug rods.

10. A process for simultaneously filtering, clarifying, concentrating and replacing a feline parvovirus stock solution, characterized in that: The following steps are involved: Step 1: first add the centrifuged virus stock solution into the virus stock solution bottle, then connect the virus stock solution bottle to the virus stock solution inlet at the bottom of the filter barrel in the filtration mechanism through a connecting hose, and then connect the two ends of the connecting hose installed with the peristaltic pump 1 to the filtrate outlet at the top of the filter barrel and the filtrate inlet at the top of the bottle body in the concentrate bottle, respectively. The virus stock solution bottle is connected to the concentrate bottle through the peristaltic pump 1 and the filtration mechanism, and the centrifuged virus stock solution is filtered and flows into the concentrate bottle. Multiple bottles of virus stock solution can be prepared. After one bottle of virus stock solution is filtered, the virus stock solution bottle is replaced by the clamping mechanism to continue the liquid replenishment; Step 2: After the concentrate bottle and the virus stock solution bottle are connected through the peristaltic pump 1 and the filtering mechanism, the concentrate outlet opened at the top of the bottle body in the concentrate bottle is connected to the hose connector connected to the liquid inlet in the concentrating mechanism through the connecting hose and the peristaltic pump 3, and the concentrate inlet opened at the top of the bottle body in the concentrate bottle is connected to the hose connector connected to the liquid outlet in the concentrating mechanism through another connecting hose. The liquid in the concentrate bottle is connected to the concentrating membrane package through the peristaltic pump 3, so as to concentrate the filtered virus stock solution; Step three, while filtering and concentrating the virus stock solution, connect the replacement fluid inlet opened at the top of the bottle body in the concentrate bottle to the replacement fluid bottle through a connecting hose and peristaltic pump 2. During the concentration process, the concentrate bottle and the replacement fluid bottle are connected through peristaltic pump 2, so that the concentrated liquid is replaced at the same time. Similar to step one, multiple bottles of replacement fluid can be prepared. After the replacement of the concentrated liquid in one bottle of replacement fluid and the concentrated liquid in the concentrate bottle is completed, the concentrate bottle is replaced by a clamping mechanism for fluid replenishment.

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