Application of rice Os-ER-ANT1 gene

Abstract

The invention relates to an application of a rice Os-ER-ANT1 gene. The nucleotide sequence of the gene is shown as SEQ ID No.1, and the coded amino acid sequence is shown as SEQ ID No.2. The gene can regulate rice light respiration amino acid metabolism, can be used for rice amino acid metabolism improved breeding, and provides a new genetic resource for improving crop quality and yield.

Description

Technical Field

[0001] The invention belongs to the field of molecular biology, and particularly relates to application of a rice gene Os-ER-ANT1 for regulating amino acid metabolism in improving crop quality and yield. Background Art

[0002] Rice yield is affected by many factors, including the number of effective panicles, the number of grains per panicle, and the thousand-grain weight. At the same time, the photosynthesis efficiency of rice is also an important factor affecting yield. However, photorespiration in the photosynthesis process is generally considered to consume energy and reduce carbon assimilation efficiency. Nevertheless, photorespiration also has a protective effect on plant adaptation to environmental changes, such as slowing down photoinhibition, rapidly adapting to low CO2 environments, promoting nitrogen assimilation, and coupling amino acid metabolism.

[0003] Photorespiration is a complex metabolic process involving multiple organelles such as chloroplasts, peroxisomes, and mitochondria. In this process, ribose-1,5-bisphosphate carboxylase / oxygenase (Rubisco) is the main enzyme involved. It catalyzes the carboxylation reaction of ribose-1,5-bisphosphate to produce 3-phosphoglycerate (3-PGA) under CO2 conditions, and oxygenates under oxygen conditions to produce 3-PGA and 2-phosphoglycolate (2-PG). The process of photorespiration converting 2PG into 3-PGA is accompanied by energy consumption, but it can also interact with glycine and serine produced by photorespiration through amino acid metabolism, coupling nitrogen assimilation, and thus play an important physiological role.

[0004] The endoplasmic reticulum (ER) also plays a key role in photorespiratory metabolism, and the ER-localized ATP / ADP transporter (Os-ER-ANT1) is believed to provide ATP to the ER. Os-ER-ANT1 deletion mutants in Arabidopsis and rice exhibit typical photorespiratory phenotypes in air, such as growth inhibition or dwarf phenotypes, but can grow normally under high CO2 conditions. In addition, the Arabidopsis er-ant1 mutant accumulates glycine due to the inhibition of glycine decarboxylase (GDC), which has an adverse effect on plant development, but this glycine accumulation is not the only reason for its dwarf phenotype. Studies have shown that the endoplasmic reticulum plays an important role in photorespiratory metabolism, but the specific physiological function of Os-ER-ANT1 and its effect on amino acid metabolism are not yet fully understood.

[0005] The key enzyme steps in the photorespiration pathway are distributed in multiple subcellular organelles, and amino acid metabolism plays an important role in the metabolic connection between organelles. However, the role of Os-ER-ANT1 in connecting photosynthesis and amino acid metabolism remains unclear.

[0006] This study comprehensively analyzed the amino acid metabolism of the rice er-ant1 mutant under air, elevated CO2 conditions, and transition from high CO2 to air, revealing the potential mechanism by which ER-ANT1 may be involved in amino acid metabolism under photorespiration conditions, providing new insights into the role of endoplasmic reticulum ATP / ADP transporters in rice photorespiration and amino acid metabolism. The present invention relates to a rice gene Os-ER-ANT1 that regulates amino acid metabolism, providing a new gene resource for genetic improvement of rice amino acid metabolism, and is expected to be used to breed new rice varieties with high photosynthetic efficiency and high yield. Summary of the invention

[0007] The purpose of the present invention is to provide an application of the rice gene Os-ER-ANT1. The Os-ER-ANT1 gene plays an important role in the photorespiration metabolism of rice, and regulates amino acid metabolism by affecting the transport and energy metabolism of ATP / ADP. Therefore, the cloning of the gene can not only analyze the molecular mechanism of rice amino acid metabolism regulation, but also provide new gene resources for the genetic improvement of crop amino acid metabolism.

[0008] The present invention is achieved through the following technical solutions:

[0009] The present invention provides an application of the Os-ER-ANT1 gene in regulating the amino acid metabolism of rice photorespiration. The nucleotide sequence and amino acid sequence of the gene are as follows:

[0010] A. Nucleotide sequence: the DNA sequence shown in SEQ ID NO: 1;

[0011] B. Amino acid sequence: the amino acid sequence shown in SEQ ID NO:2.

[0012] The use of knockout vectors, overexpression vectors, transgenic strains and recombinant bacteria containing the above-mentioned ER-ANT1 gene also falls within the protection scope of the present invention.

[0013] The present invention utilizes a mutant material Os-ER-ANT1 of the Os-ER-ANT1 gene (LOC_Os11g43960) of rice that is targeted for knockout. The mutant exhibits growth retardation and abnormal amino acid metabolism in the air, especially abnormal accumulation of glycine, glutamate and alanine. Experimental results show that under high CO2 conditions, the amino acid metabolism of the mutant is partially restored, while in the air it exhibits a dramatic change in amino acid content, especially accumulation of glycine. The Os-ER-ANT1 gene is an adenylate transporter located in the endoplasmic reticulum, indicating that Os-ER-ANT1 is a key regulatory factor in amino acid metabolism. There is currently no research report on Os-ER-ANT1 in rice photorespiration amino acid metabolism, so the target gene Os-ER-ANT1 of the present invention is a new gene that regulates rice amino acid metabolism.

[0014] The application of the above rice Os-ER-ANT1 gene also includes the application of the gene in rice amino acid metabolism improvement breeding. The methods of rice amino acid metabolism improvement breeding include genetic engineering breeding and hybrid breeding, and the method is: genetic engineering breeding, introducing a knockout vector containing the Os-ER-ANT1 gene into plant cells, and obtaining materials with changed amino acid metabolism by knocking out the Os-ER-ANT1 gene in the plant, for example, knocking out the Os-ER-ANT1 gene in rice can cultivate rice varieties with abnormal photorespiration amino acid metabolism. Hybrid breeding: using the above amino acid metabolism mutant rice material as a parent, improving rice amino acid metabolism through hybrid breeding, that is, by hybridizing the obtained er-ant1 mutant rice with the rice variety to be improved, the targeted improvement of rice amino acid metabolism is carried out.

[0015] The present invention has the following advantages and effects:

[0016] 1. ER-ANT1 gene deletion manifests as inactivation of rice Os-ER-ANT1 gene, and phenotypic analysis shows that mutants have slow growth and abnormal amino acid metabolism. This provides new gene resources for genetic improvement of rice amino acid metabolism and new target genes for rice molecular design breeding.

[0017] 2. There is no research on the function of Os-ER-ANT1 gene in regulating rice amino acid metabolism. Therefore, the target gene Os-ER-ANT1 of the present invention is a new gene that regulates rice amino acid metabolism. It has important theoretical significance in revealing the mechanism of amino acid metabolism and provides a new example for broadening the existing understanding of the molecular mechanism of rice amino acid metabolism regulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The phenotypes of the wild type and er-ant1 mutant and the amino acid content of their leaves in Example 1, Figures A and B are the phenotypes of the wild type WT and er-ant1 mutant after 15 and 30 days of cultivation, Figure C is the amino acid content measured in the leaves of plants harvested at 6 pm after 12 days of growth under natural conditions, and Figure D is the amino acid content in the leaves of er-ant1 and wild type plants grown under high CO2, among which the wild type WT is Zhonghua11.

[0019] Figure 2 This is a comparison of the free amino acid content of the wild type and er-ant1 mutant under different photorespiration conditions in Example 2; Figure A shows the changes in total amino acid content of the wild type and er-ant1 plants after being treated with high CO2 conditions for 12 hours and transferred to natural conditions, Figure B shows the proportion of each amino acid, and Figure C shows the changes in the content of glycine, valine, isoleucine, leucine, phenylalanine and histidine.

[0020] Figure 3 Figure 2 shows the changes in glutamate content in the wild type and er-ant1 mutant of Example 2; Figure A shows the changes in glutamate and glutamine content, Figure B shows the activity and mRNA level of Fd-GOGAT, Figure C shows the comparison of the whole plant and leaf phenotypes of the control ZH8015 and es7 mutants grown in paddy fields, and Figure E shows the amino acid content of 3-week-old ZH8015 and es7 leaves in ambient air.

[0021] Figure 4 Figure 2 shows the correlation between alanine content, AGAT1 activity and AGAT1 mRNA level; Figure A shows the phylogenetic evolutionary tree of 8 transaminases in Arabidopsis and rice that are speculated to be related to photorespiration, Figure B shows the changes in alanine content in wild type and er-ant1 plants, Figure C shows the expression level of Os-AGAT1 gene, and Figure D shows the comparison of alanine content, AGAT1 transcription level and AGAT1 enzyme activity between the wild type and er-ant1 mutant 7 days after being transferred from a high CO2 environment to air.

[0022] Figure 5 This is the phosphoserine pathway of serine synthesis induced by er-ant1 under photorespiration conditions in Example 2; Figure A shows the changes in serine content in leaves of wild type and er-ant1 mutants, and Figure B shows the gene expression analysis of genes encoding phosphoserine phosphatase (PSP), phosphoserine aminotransferase (PSAT) and 3PGA dehydrogenase (3-PGDH) under high CO2 and natural conditions.

[0023] Figure 6 NAD of wild type and er-ant1 plants during CO2 transfer in Example 3 + and NADH content; plants were grown under high CO2 conditions for 2 weeks and then transferred to natural conditions, and leaf materials were harvested at 0, 1, 8 and 12 h.

[0024] Figure 7 Figure 4 shows the phenotypes of the wild type and er-ant1 mutant grown in a high CO2 environment and transferred to ambient air; Figure A shows the phenotypes of the wild type and er-ant1 mutant grown in a high CO2 environment, Figure B shows the phenotypes of plants transferred to natural conditions for 2 weeks, Figure C shows the photorespiration rate measured by the Rubisco enzyme kinetic estimation method, and Figure D shows the photorespiration rate at a photon flux density of 1100 μmol m -2 s -1 , temperature is 28°C, CO2 concentration is about 400 μmol mol -1 The net photosynthetic rate was determined under the conditions of DETAILED DESCRIPTION

[0025] The present invention is further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0026] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and substance of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention all fall within the scope of protection of the present invention.

[0027] The rice variety in the embodiment is wild-type japonica rice Zhonghua 11 (a publicly used rice variety, commercially available), the pCubi1390 vector is a commonly used gene expression vector, and Escherichia coli DH5α and Agrobacterium tumefaciens EHA105 are commonly used strains, which are stored in most molecular biology laboratories.

[0028] The main reagents in the embodiment are: restriction endonucleases, Taq enzymes, T4 ligases, Pyrobest Taq enzymes, KOD, etc. are purchased from TAKARA (Dalian), Promega, NEB, ABM and other biological companies; plasmid mini-extraction kits and agarose gel recovery kits are purchased from Shanghai Jierui Bioengineering Co., Ltd.; culture medium, agar powder, agarose, ampicillin (Amp), kanamycin (Kan), rifampicin (Rif) and other antibiotics are purchased from Sigma; other chemical reagents used in the embodiment are imported or domestic analytical grade. The primers used in the embodiment were synthesized by Beijing Ruibo Xingke Biotechnology Co., Ltd., and sequencing was performed at Beijing Qingke Biotechnology Co., Ltd.

[0029] The test methods involved are as follows:

[0030] Gas exchange measurements

[0031] Gas exchange measurements were performed using the GFS-3000 system under high CO2 conditions (i.e., non-photorespiratory conditions) in a closed artificial climate chamber with continuous dry ice, a temperature range of 28–30°C, a relative humidity range of 70–90%, and a photon flux density range of ∼300 μmol m -2 s -1 After 2 weeks of growth, the youngest fully expanded leaf of each three-leaf seedling was used for gas exchange measurement. Under normal growth conditions (i.e., photorespiration conditions, temperature setting: 28°C, relative humidity: 70%, photon flux density: 1100 μmol m -2 s -1 , CO2: 400 μmol mol -1 ), measuring the leaf net CO2 assimilation rate (A net ) and intercellular CO2 concentration (C i). Mitochondrial respiration rate under light (R d ) is divided by the nighttime dark respiration rate (R n ), which was measured in the dark. The photorespiration rate (P R ),

[0032]

[0033] where Γ * It is the CO2 compensation point of the chloroplast carboxylation site. c is the chloroplast CO2 concentration, calculated using the formula:

[0034]

[0035] Among them, the leaf conductivity (g m =0.45 mol m -2 s -1 ).

[0036] Leaf Fd-GOGAT activity assay

[0037] Soluble total protein was extracted from rice leaves at 0-4°C using HEPES buffer (50 mmol L -1 , pH 7.5), 0.1% β-mercaptoethanol, 1 mM ethylenediaminetetraacetic acid (EDTA) and 5 mmol L -1 MgCl2. Fd-GOGAT activity was determined using reduced methylpurpurin as an electron donor. The reaction mixture consisted of 50 mmol L -1 HEPES (pH 7.5), 10 mmol L -1 Glutamine, 10 mmol L -1 2-Ketoglutarate, 15mmol L -1 After pre-incubation at 30°C for 5 min, a reducing agent solution (12 mmol L -1 Na2S2O4, 13mmol L -1 The reaction was started by adding 5% NaHCO3. After incubation at 30°C for 20 min, 0.5 mL (6%) salicylsulfonic acid was added and the reaction was terminated by shaking vigorously. The activity of Fd-GOGAT was calculated by measuring the increase of glutamate by amino acid analyzer. In each test, a blank control (without glutamine) was added.

[0038] Leaf AGAT activity assay

[0039] The assay for AGAT activity is similar to that for GGAT activity, the only difference being that the amino donor (alanine) and pyruvate dehydrogenase as the coupling enzyme are modified. The assay for AGAT includes 10 mmol L -1 L-alanine, 10 mmol L -1 Glyoxylate and 100 μL crude enzyme (for enzyme assays) or 100 μL 50 mmol L -1 PBS (potassium salt, pH 7.4) was used as a control, and 100 mmol L - 1 0.5 U pyruvate dehydrogenase and 0.25 mmol L -1 CoA and 0.2 mmol L -1 NADH, the final volume is 0.5 mL.

[0040] Amino acid content determination

[0041] Rice leaves were cut with surgical scissors, quickly frozen in liquid nitrogen, and then ground into powder in a mortar with a pestle. 0.2 g of the powder sample was extracted with 2 mL of 6% (w / v) 5-salicylic sulfonic acid on ice for 40 min. Total free amino acids were determined using an amino acid analyzer equipped with a chromatographic column and a pH-dependent lithium buffer gradient elution program at 570 and 440 nm wavelengths for 148 min. For each amino acid, the average of three replicates was calculated, each consisting of 10-12 plants.

[0042] NADH / NAD + Ratio determination

[0043] NAD + The NADH and NAD were determined using the Nanjing Jiancheng quantitative kit. The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide) reduction method was used to determine the NAD + and NADH content. Rice leaves (~200 mg) were collected after first growing in a high C02 environment and then in ambient air for 0, 1, 8, and 12 hours. The tissue was first ground in an ice bath, extracted with 2 mL of NADH / NAD+ alkaline / acidic extract, and boiled for 5 minutes. After cooling in ice, the samples were centrifuged at 10,000×g for 10 min at 4°C. The supernatant was then transferred to a new centrifuge tube and mixed with the corresponding volume of extract for neutralization. The mixture was centrifuged at 10,000×g for 10 min at 4°C, and the supernatant was taken for detection. 200 μL of the liquid supernatant of the sample was mixed with reagent A, reagent B, and reagent C and allowed to stand at room temperature for 25 min. The sample was centrifuged at 20,000×g for 5 min, the precipitate was mixed with reagent D, and the absorbance of each tube was measured at 570 nm. NAD +The calculation formula of NADH content is as follows:

[0044] NAD + content(nnol Q -1 FW)=(A nm -A connol -0.099)×36.1 / (W / V)

[0045] NADH content (mmol g -1 FW)=(A test ·Ac ontrol -0.065)×24.7 / ((W / V)W is the fresh weight of the sample to be tested, and V is the volume of the extract (2ml).

[0046] In the following examples, the method described in the example of patent CN104673804B was first used to obtain the er-ant1 mutant from Zhonghua 11 rice by targeted knockout of the Os-ER-ANT1 gene (LOC_Os11g43960) of rice. an The phenotypes and amino acid metabolism of the t1 mutant and conventional wild-type WT plants (Zhonghua11) were comprehensively analyzed to reveal the potential mechanism by which ER-ANT1 may be involved in amino acid metabolism under photorespiration conditions.

[0047] Example 1 Phenotypes of wild type and er-ant1 mutant and amino acid content in their leaves

[0048] Wild type WT and O s-e r- an The t1 mutant plants were photographed at 15 and 30 days of growth under natural conditions. Figure 1 As shown, Figure A shows the phenotype of wild-type WT and er-ant1 mutant after 15 days of culture, and Figure B shows the phenotype after 30 days of culture.

[0049] The samples were grown for 12 days under natural conditions and under high CO2 (i.e., non-photorespiratory conditions) in a closed artificial climate chamber with continuous dry ice, temperature range of 28-30°C, relative humidity range of 70-90%, photon flux density range of 300 μmol / m -2 s -1 The amino acid content of leaves of wild-type WT and Os-er-ant1 mutant plants grown in 24 h was analyzed. Figure 1As shown in C and D. It can be seen that under photorespiratory conditions (natural conditions, ~0.4% CO2), the er-ant1 mutant showed slow growth and abnormal amino acid metabolism; but under non-photorespiratory conditions (high CO2 conditions, 1.5% CO2), the content of most amino acids in the mutant was no different from that in the wild type.

[0050] Example 2 Analysis of amino acid content of wild type and er-ant1 mutant under different photorespiration conditions.

[0051] To better understand the metabolic changes associated with the photorespiratory phenotype of the er-ant1 mutant, the ER-ant1 mutant was grown in a closed artificial climate chamber under high CO2 conditions (i.e., non-photorespiratory conditions) with continuous dry ice addition, temperature range of 28-30°C, relative humidity range of 70-90%, and photon flux density range of ∼300 μmol m -2 s -1 ) for 12 hours and then transferred to natural conditions (~0.4% CO2). The amino acid changes in leaves of wild-type and er-ant1 plants at different times after transfer to natural conditions were detected, while ZH8015 and es7 mutants grown in paddy fields were used as controls. Figure 3 C and D are the comparisons of the whole plant and leaf phenotypes of ZH8015 and es7 mutants grown in paddy fields, respectively.

[0052] Figure 2 The free amino acid content of leaves of wild-type and er-ant1 plants at different times after transfer to natural conditions is shown, among which Figure A shows the change of total amino acid content. Figure B shows the ratio of each amino acid. Figure C shows the change of glycine, valine, isoleucine, leucine, phenylalanine and histidine content.

[0053] Figure 3 A shows the changes in glutamate and glutamine content in leaves of wild-type and er-ant1 plants at different times after transfer to natural conditions. B shows the activity and mRNA level of Fd-GOGAT. E shows the amino acid content in the leaves of the 3-week-old control group ZH8015 and es7 in ambient air.

[0054] Figure 4 Figure 1 is the correlation between alanine content, AGAT1 activity and AGAT1 mRNA level. Figure A is the phylogenetic evolutionary tree of 8 transaminases in Arabidopsis and rice that are speculated to be related to photorespiration. Figure B shows the changes in alanine content in wild-type and er-ant1 plants. Figure C shows the expression level of Os-AGAT1 gene. Figure D shows the comparison of alanine content, AGAT1 transcription level and AGAT1 enzyme activity between wild-type and er-ant1 mutants after 7 days of transfer from high CO2 environment to air.

[0055] Figure 5Figure 1 shows the phosphoserine pathway for serine synthesis induced by er-ant1 under photorespiration conditions. Figure A shows the changes in serine content in leaves of wild type and er-ant1 mutant. Figure B shows the gene expression analysis of genes encoding phosphoserine phosphatase (PSP), phosphoserine aminotransferase (PSAT) and 3PGA dehydrogenase (3-PGDH) under high CO2 and natural conditions.

[0056] Combination Figure 2-5 , it can be seen that the total free amino acid content of the wild type and er-ant1 at 0h was similar. However, after being transferred to natural conditions, the amino acid content of the wild type and er-ant1 leaves changed differently. Among them, the content of glutamate (Glu), glutamine (Gln) and alanine (Ala) decreased; the serine level decreased initially, and serine accumulated after one week. Alanine:glyoxylate aminotransferase AGAT is a key enzyme in the glycine biosynthesis pathway that catalyzes the conversion of alanine to glycine. AGAT1 is an endoplasmic reticulum-localized protein that exhibits higher activity in the er-ant1 mutant, which may be the reason for the reduced alanine level and the large accumulation of glycine (Gly).

[0057] Example 3 NAD in wild-type and er-ant1 plants during CO2 transfer + and NADH content.

[0058] Wild-type and er-ant1 plants were grown under high CO2 conditions for 2 weeks and then transferred to natural conditions. Leaf material was harvested at 0, 1, 8, and 12 h and tested for NAD + and NADH content, the results are as follows Figure 6 shown.

[0059] Glycine from photorespiration is the main source of NADH in leaves. + The results showed that under high CO2 conditions, there was no difference in NADH between the wild type and er-ant1, while the NAD + After transfer to natural conditions, NADH in the wild type increased, while that in the er-ant1 mutant was even higher; + The content of NAD increased, while in er-ant1 mutant + Almost no change ( Figure 6 ), which indicates that NAD + The above results show that the loss of Os-ER-ANT1 affects the metabolism of NAD + Metabolism, resulting in NADH / NAD + The ratio increases.

[0060] Example 4 Phenotypes of wild type and er-ant1 mutant grown in a high CO2 environment and transferred to ambient air.

[0061] The wild-type and er-ant1 plants were grown under high CO2 conditions for 2 weeks and then transferred to natural conditions for phenotype analysis to test the photorespiration rate and net photosynthetic rate.

[0062] Figure 7 Figure A shows the wild type and er-ant1 mutant grown under high CO2 conditions, and Figure B shows the phenotype of plants transferred to natural conditions for 2 weeks. Figure C shows the photorespiration rate of wild type and er-ant1 plants after 2 weeks of transfer to natural conditions, measured by Rubisco enzyme kinetic estimation. Figure D shows the photorespiration rate of wild type and er-ant1 plants under photon flux density of 1100 μmol m -2 s -1 , temperature is 28℃, CO 2 The concentration is about 400 μmol mol -1 The net photosynthetic rates of wild-type and er-ant1 plants were measured under conditions of 40 % and 80 % respectively and transferred to natural conditions for 2 weeks.

[0063] It can be seen that under high CO2 conditions, the growth of the er-ant1 mutant is similar to that of the wild-type plants; however, after about two weeks of exposure to ambient air, the er-ant1 mutant wilts and almost dies. Under natural growth conditions, the photorespiration and net photosynthetic rate of the er-ant1 mutant are inhibited.

[0064] In summary, the present invention concludes that the rice er-ant1 mutant has slow growth and abnormal amino acid metabolism, but under high carbon dioxide conditions (1.5% CO2), the content of most amino acids in the mutant is not significantly different from that in the wild type. + The increase in the ratio leads to a decrease in GDC activity and a blockage in the decomposition of glycine, thereby reducing downstream metabolites such as serine. The above shows that Os-ER-ANT1 is a housekeeping gene with important functions, which plays an important regulatory role in rice amino acid metabolism and can be used in rice amino acid metabolism improvement breeding.

[0065] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

[0066] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0067] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. Application of rice Os-ER-ANT1 gene in regulating rice photorespiration amino acid metabolism, characterized in that: The nucleotide sequence of the Os-ER-ANT1 gene is shown in SEQ ID No.1, and the encoded amino acid sequence is shown in SEQ ID No.

2.

2. The use according to claim 1, characterized in that The application is to regulate the amino acid metabolism of rice photorespiration by inhibiting or knocking out the expression of the Os-ER-ANT1 gene.

3. Application of rice Os-ER-ANT1 gene in rice amino acid metabolism improvement breeding, the nucleotide sequence of the Os-ER-ANT1 gene is shown in SEQ ID No.1, and the encoded amino acid sequence is shown in SEQ ID No.

2.

4. The use according to claim 3, characterized in that: The application is to knock out the Os-ER-ANT1 gene in plants through genetic engineering.

5. The use according to claim 3, characterized in that: The application is to use the plant with Os-ER-ANT1 gene knocked out as a parent to hybridize with the rice to be improved through hybrid breeding.

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