Sunscreen composition for protection of lactobacillus and staphylococcus epidermidis on skin

Abstract

The present invention relates to a selected ultraviolet (UV) filter selected from the group consisting of octocrylene, ethylhexyltriazinone and butylmethoxydibenzoylmethane for use in the protection of skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus, preferably in the copresence of Staphylococcus epidermidis, from UV radiation, preferably in the copresence of Staphylococcus epidermidis, for example Lactobacillus crispatus, preferably in the copresence of Staphylococcus epidermidis, for use in the protection of skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus, preferably in the copresence of Staphylococcus epidermidis, for use in the protection of skin bacteria of the genus Lactobacillus, such as Lactobacillus crispatus, preferably in the copresence of Staphylococcus epidermidis. Use of the composition to promote maintenance of healthy skin microbiome following UV irradiation.

Description

[0001] The present invention relates to the use of selected ultraviolet (UV) filters selected from the group consisting of octocrylene, ethylhexyltriazone and butyl methoxydibenzoylmethane for protecting skin bacteria of the genus Lactobacillus, such as L. crispatus, preferably in the simultaneous presence of Staphylococcus epidermidis, from UV radiation to promote the maintenance of a healthy skin microbiome after UV irradiation.

[0002] The skin is the outermost protective layer of an organism and is the largest organ in the human body. It acts as a barrier and protects the body from external factors. It is also well known that the skin surface is inhabited by a variety of microorganisms that form the skin microbiome (often also referred to as the skin microbiome). Most of these microorganisms are symbiotic organisms that live in a mutually beneficial symbiotic relationship with the skin's immune system. Dysbiosis of the skin microbiome is associated with many skin pathologies, such as acne, atopic dermatitis, and psoriasis.

[0003] Endogenous inhabitants of healthy skin are lactobacilli (Lactobacillus family), such as Lactobacillus crispatus. They have been described as playing a beneficial role in the skin habitat, where they play a multifactorial local mechanism of action against pathogens and inflammation. On the one hand, lactobacilli have been shown to compete directly with skin pathogens by inhibiting adhesion, producing antimicrobial metabolites, and by affecting pathogen metabolism. The competitive antipathogenic effects of lactobacilli have been mechanistically described for common different skin pathogens, such as Staphylococcus aureus, Propionibacterium acnes, and Candida albicans. On the other hand, lactobacilli also have the immunomodulatory ability associated with reducing excessive skin inflammation. Their effects on the immune system are mediated by bacterial metabolites and cell wall-related or excretion microorganism-associated molecular patterns (MAMPs). In addition, lactobacilli can also enhance skin barrier function, such as when the skin barrier function is destroyed due to infection or inflammatory skin diseases.

[0004] It is well known that UV light destroys microorganisms such as bacteria. Therefore, UV radiation may induce significant changes in the skin microbiome and thereby may alter the immune response and / or lead to a compromised skin barrier.

[0005] Therefore, there is a continuing need to selectively protect beneficial bacteria, such as skin bacteria, in particular of the genus Lactobacillus, from UV radiation. Furthermore, it is also desirable to simultaneously protect another beneficial skin commensal, Staphylococcus epidermidis.

[0006] Therefore, the inventors have developed a targeted in vitro liquid model in a 96-well microplate format to screen for UV protection active substances ("UV filters") that enable bacterial survival after UV irradiation. The effects of selected UV irradiation conditions tested on the microbial populations Lactobacillus crispatus and Staphylococcus epidermidis in the presence of UV filter samples and combinations thereof were evaluated by the evolution of the residual microbial population after 4 h. The bacterial growth inhibition as a result of UV irradiation was compared with that of the same strains without UV irradiation and expressed as a percentage (%) of survival. In addition, a clinical single-blind placebo-controlled trial specifically evaluated the effects of UV irradiation on the composition of the skin microbiome of human skin and the protection of the skin microbiome by a representative SFP20 sunscreen versus a placebo formulation.

[0007] Surprisingly, it has now been found that certain UV filter substances are particularly suitable for protecting skin bacteria of the genus Lactobacillus, such as in particular Lactobacillus crispatus, more preferably in the simultaneous presence of Staphylococcus epidermidis, from the harmful effects of UV radiation by significantly and selectively increasing the survival rate of skin bacteria of the genus Lactobacillus, such as in particular Lactobacillus crispatus, more preferably in the simultaneous presence of Staphylococcus epidermidis after irradiation with UV light.

[0008] Thus, in a first embodiment, the present invention relates to a method for protecting bacteria of the genus Lactobacillus, preferably in the simultaneous presence of Staphylococcus epidermidis, from the harmful effects of UV radiation, said method comprising the step of applying to an external surface of a human body having a skin microbiota comprising said bacteria, before exposing said external surface to UV radiation, an effective amount of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone.

[0009] In another embodiment, the present invention relates to the use of one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone for protecting bacteria of the genus Lactobacillus from the harmful effects of UV radiation, preferably in the simultaneous presence of Staphylococcus epidermidis.

[0010] In an additional embodiment, the present invention is directed to a method for increasing the differential abundance of bacteria of the genus Lactobacillus in the skin microbiome of an individual after exposure to UV radiation, preferably in the simultaneous presence of Staphylococcus epidermidis, the method comprising the step of topically applying to an external surface of a body in need thereof, prior to exposing the external surface to UV radiation, an effective amount of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone.

[0011] In another embodiment, the present invention also provides a method for protecting the external surfaces of the human body by selectively increasing the differential abundance of skin bacteria of the genus Lactobacillus after exposure to UV radiation, preferably in the presence of Staphylococcus epidermidis in the skin microbiome, the method comprising the step of topically applying to the external surface a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone before exposure to UV radiation.

[0012] In another embodiment, the present invention relates to the use of one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone, or a sunscreen composition comprising one or more of said UV filters, for selectively increasing the differential abundance of skin bacteria of the genus Lactobacillus after exposure to UV radiation, preferably in the presence of Staphylococcus epidermidis in the skin microbiome of an individual in need thereof.

[0013] In another embodiment, the invention relates to a composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone for use in the treatment or prevention of dysbiosis and / or for strengthening the surface barrier of the skin after UV radiation.

[0014] All methods and uses disclosed herein are particularly suitable for treating or preventing dysbiosis and / or strengthening the surface barrier of the skin after UV radiation. The compositions, methods and uses as disclosed herein are suitable for maintaining a healthy and well-balanced skin microbiome after UV radiation.

[0015] Since the number of bacteria of the genus Lactobacillus increases, ie the abundance increases, after UV irradiation, the metabolites secreted by said bacteria may also increase.

[0016] It is well known to those skilled in the art that compositions comprising UV filters may also be referred to as sunscreen compositions.

[0017] Without wishing to be bound by theory, these metabolites are believed to contribute to the body's natural defence system. Thus, in another aspect, the invention relates to the use of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone for improving the natural defence system of a surface of the human body by the balance of the microbiota after exposure of the surface to UV radiation.

[0018] In a further aspect, the present invention also relates to a method for improving the natural defence system of a surface of the human body by balancing the microbiota, said method comprising the steps of applying to said surface a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone before exposing said surface to UV radiation, then exposing said surface to UV radiation and optionally appreciating the beneficial effects produced thereby.

[0019] The protection according to the invention against skin bacteria of the genus Lactobacillus, such as in particular Lactobacillus crispatus, is characterized in that after exposure to UV radiation (at 50 SED), the survival of skin bacteria of the genus Lactobacillus, such as in particular Lactobacillus crispatus, is increased by at least 100% compared to a control.

[0020] In an additional and advantageous embodiment, the S. epidermidis are also simultaneously protected from UV radiation. Said protection is characterized in that the survival rate of the S. epidermidis after exposure to UV radiation (at 15 SED) is increased by at least 20% compared to a control.

[0021] Particularly preferred UV filters according to the invention are octocrylene and a mixture of ethylhexyl triazone, butyl methoxydibenzoylmethane and bis-ethylhexyloxyphenol methoxyphenyl triazine, since they lead to particularly good survival of skin bacteria of the genus Lactobacillus, in particular Lactobacillus crispatus.

[0022] The most preferred Lactobacillus species in all embodiments of the present invention is Lactobacillus crispatus CIP 103604.

[0023] The most preferred Staphylococcus epidermidis in all embodiments of the present invention is Staphylococcus epidermidis ATCC 12228.

[0024] The total amount of UV filters in the sunscreen composition depends largely on the targeted protection and the UV radiation to be exposed to. Preferably, in all embodiments of the invention, the amount of UV filters is chosen such that the sunscreen composition exhibits an SPF of at least 20, preferably at least 25, most preferably at least 30, for example in the range of 20 to 100, 20 to 50, 25 to 100, 25 to 50, 30 to 100 or 30 to 50.

[0025] For example, a sunscreen composition of SPF 20 comprises UV filters in a total amount of preferably between 10 and 40 wt.-%, more preferably between 15 and 25 wt.-%, based on the total weight of the composition.

[0026] For example, a sunscreen composition of SPF 50 comprises UV filters in a total amount of preferably between 15 and 50 wt.-%, more preferably between 20 and 40 wt.-%, based on the total weight of the composition.

[0027] In all embodiments of the invention, a particularly preferred bacterium of the Lactobacillus species is Lactobacillus crispatus.

[0028] As used herein the term UV radiation (used synonymously with UV light) refers to radiation in the UVA-UVB range of 280 nm to 400 nm.

[0029] The term 'external surface of the human body' as used herein refers to the skin as well as the scalp. Preferably, in all embodiments of the present invention, the external surface of the human body treated according to the present invention is the face, neck and / or body skin.

[0030] The term 'skin microbiome' as used herein refers to the microbial population that colonizes a defined skin area of ​​an individual, such as, but not limited to, the forehead, forearms, cheeks, or scalp.

[0031] As used herein, the term 'differential abundance' refers to the logarithm of the fold change in the abundance of a taxon between two conditions. This analytical method gives differences as output, allowing identification of microbiome taxa associated with certain biological or clinical conditions. The differences can be ranked and sorted from lowest to highest. These "rankings" give information about the relative association of a feature with a given covariate (i.e., treatment or time).

[0032] As used herein, 'differential abundance' is determined by using a software called Songbird as listed in Morton et al. (Nat. Commun., 2019.10(1): p. 2719), which is included herein by reference, which allows the establishment of statistical model tests to take into account differences between treatments and calculate differences starting from relative abundance data from 16S rRNA sequencing. The output is a file containing the ranking of features of certain metadata categories. The higher the ranking, the greater its relevance to that category (i.e., treatment).

[0033] The term 'increase in differential abundance' as used herein is defined as an increase in the differential abundance of a particular microorganism when compared to the differential abundance of a corresponding symmetrical area of ​​skin from the same individual treated with a placebo.

[0034] In all embodiments of the present invention, the amount of ethylhexyl triazone (also known as 2,4,6-tris[anilino(p-carbon-2'-ethyl-1'-hexyloxy)]-1,3,5-triazine (EHT)) in the sunscreen composition according to the present invention is preferably selected from the range of 0.1 wt% to 10 wt%, more preferably from the range of 0.5 wt% to 7.5 wt%, most preferably from the range of 1 wt% to 5 wt%, based on the total weight of the sunscreen composition. Further suitable ranges are 1 wt% to 5 wt%, 1.5 wt% to 4 wt%, or 2 wt% to 3 wt%.

[0035] In all embodiments of the present invention, the amount of butyl methoxydibenzoylmethane (also known as avobenzone, or 3-(4-tert-butylphenyl)-1-(4-methoxyphenyl)propane-1,3-dione (BMDBM)) in the sunscreen composition according to the present invention is preferably selected from the range of 0.1% to 10% by weight, more preferably from 0.5% to 7.5% by weight, most preferably from 1% to 5% by weight, based on the total weight of the sunscreen composition. Other suitable ranges are 2% to 7.5% by weight, or 3% to 7% by weight, or 4% to 6% by weight.

[0036] In all embodiments of the present invention, the amount of octocrylene (also known as 2-ethylhexyl 2-cyano-3,3-diphenylprop-2-enoate (OC)) in the sunscreen composition according to the present invention is preferably selected from the range of 0.1% to 10% by weight, more preferably from 0.5% to 7.5% by weight, most preferably from 1% to 5% by weight, based on the total weight of the sunscreen composition. Other suitable ranges are 2% to 7.5% by weight, or 3% to 7% by weight, or 4% to 6% by weight.

[0037] In a particular embodiment, the sunscreen composition according to the invention comprises octocrylene, ethylhexyl salicylate, bis-ethylhexyloxyphenol methoxyphenyl triazine and butyl methoxydibenzoylmethane, more preferably in an amount of 15-20 wt %, based on the total weight of the sunscreen composition. Such a composition is particularly suitable for increasing the differential abundance of bacteria of the genus Lactobacillus, such as in particular Lactobacillus crispatus, in the skin microbiota of an individual after exposure of the skin microbiota to UV radiation.

[0038] If a combination of ethylhexyl triazine, butyl methoxydibenzoylmethane and bis-ethylhexyloxyphenol methoxyphenyl triazine is used, the amount of each of the UV filters is preferably selected from the range of 1 wt.-% to 5 wt.-%, more preferably from 2 wt.-% to 4 wt.-%, most preferably from about 3 wt.-%, based on the total weight of the composition.

[0039] The UV filters to be used according to the invention are preferably contained in a composition comprising one or more oils suitable for dissolving and / or diluting the UV filters in an amount as indicated herein. Such oils are well known in the art. Particularly suitable oils are selected from the group consisting of: 2-phenoxyethyl caprylate (also known as XC) and caprylic / capric / succinic triglycerides (also known as 829Eco). The composition may also contain an emulsifier, such as preferably polysorbate-80 (also known as polyoxyethylene-80-sorbitan monooleate, 80).

[0040] In all embodiments of the present invention, preferably, the total amount of oil in the composition according to the present invention is preferably selected from the range of 50 wt.-% to 95 wt.-%, more preferably from the range of 50 wt.-% to 90 wt.-%, most preferably from the range of 75 wt.-% to 90 wt.-%, based on the total weight of the composition.

[0041] In all embodiments of the present invention, the total amount of emulsifier in the composition according to the present invention is preferably selected from the range of 1 wt.-% to 7.5 wt.-%, more preferably from the range of 2.5 wt.-% to 7.5 wt.-%, most preferably from the range of 3 wt.-% to 6 wt.-%, based on the total weight of the composition.

[0042] Since the sunscreen compositions according to the invention are intended for topical application, it is known that they comprise a physiologically acceptable medium, ie a medium compatible with keratinous materials such as the skin, mucous membranes and keratinous fibers. In particular, the physiologically acceptable medium is a cosmetically acceptable carrier.

[0043] The term 'cosmetically acceptable carrier' as used herein refers to all carriers and / or excipients and / or diluents conventionally used in topical cosmetic compositions, such as in particular in skin care preparations.

[0044] The exact amount of carrier will depend on the actual level of UV filters and any other optional ingredients that one of ordinary skill in the art would classify as different from a carrier (eg, other active ingredients).

[0045] In an advantageous embodiment, the sunscreen composition according to the invention comprises 50% to 99% by weight, preferably 60% to 98% by weight, more preferably 70% to 98% by weight, such as in particular 80% to 95% by weight, of a carrier, based on the total weight of the sunscreen composition.

[0046] In a particularly advantageous embodiment, the carrier further consists of at least 40 wt. %, more preferably at least 50 wt. %, most preferably at least 55 wt. % water, such as in particular 55 to 90 wt. % water.

[0047] In particular, the sunscreen composition according to the invention is a cosmetic composition or a pharmaceutical composition, preferably a cosmetic (non-therapeutic) composition.

[0048] In one embodiment, the sunscreen composition according to the invention is applied to mammalian keratinous tissue, such as, in particular, human skin.

[0049] As used in this application, the term "cosmetic composition" refers to Cosmetic compositions as defined under the heading "Kosmetika" in Lexikon Chemie, 10th edition, 1997, Georg Thieme Verlag Stuttgart, New York, and as disclosed in A. Domsch, "Cosmetic Compositions", Verlag für chemische Industrie (ed. H. Ziolkowsky), 4th edition, 1992.

[0050] The topical compositions according to the invention are also referred to as sun protection preparations (sun protection products, sunscreens), for example sun protection milks, sun protection lotions, sunscreen creams, sun protection oils, sun blocks or tropical creams or day creams with SPF (sun protection factor). Of particular interest are sun protection creams, sun protection lotions, sun protection milks and sun protection preparations.

[0051] The compositions of the invention (including carriers) may contain conventional adjuvants and additives, such as preservatives / antioxidants, fatty substances / oils, organic solvents, silicones, thickeners, emollients, emulsifiers, antifoaming agents, aesthetic components (e.g. fragrances), surfactants, fillers, anionic, cationic, nonionic or amphoteric polymers or mixtures thereof, propellants, acidulants or alkalizers, dyes, colorants / dyes, abrasives, absorbents, chelating and / or sequestrants, essential oils, skin sensates, astringents, pigments, or any other ingredient typically formulated into such compositions.

[0052] According to the invention, the composition according to the invention may comprise further ingredients, for example for lightening the skin; preventing tanning; treating hyperpigmentation; preventing or reducing acne, wrinkles, fine lines, atrophy and / or inflammation; chelating agents and / or chelates; anti-cellulite and slimming (e.g. phytanic acid), firming, moisturizing and revitalizing, self-tanning, soothing ingredients, as well as agents for improving elasticity and the skin barrier, and / or additional UV filters and carriers and / or excipients or diluents conventionally used in sunscreen compositions.

[0053] If not stated otherwise, the excipients, additives, diluents etc. mentioned below are suitable for use in the sunscreen compositions according to the invention. The necessary amounts of cosmetic and dermatological auxiliaries and additives can be easily determined by the skilled person based on the desired product.

[0054] The additional ingredients may be added to the oil phase, the water phase or separately as appropriate. The mode of addition can be easily adapted by those skilled in the art.

[0055] Examples of cosmetic excipients, diluents, adjuvants, additives and active ingredients commonly used in the skin care industry suitable for use in the cosmetic compositions of the present invention are described, for example, in the International Cosmetic Ingredient Dictionary & Handbook by Personal Care (http: / / www.personalcarecouncil.org / ), provided by the Personal Care Products Council, which can be accessed through the online INFO BASE (http: / / online.personalcarecouncil.org / jsp / Home.jsp), but are not limited thereto.

[0056] In some cases, the cosmetically active ingredients useful herein may provide more than one benefit or operate via more than one mode of action.

[0057] Of course, the person skilled in the art will take care to choose the above-mentioned optional additional ingredients, adjuvants, diluents and additives and / or their amounts so that the advantageous properties essentially associated with the combination according to the invention are not or are not substantially adversely affected by one or more of the envisaged additions.

[0058] The sunscreen composition according to the present invention may be in the form of a suspension or dispersion in a solvent or a fatty substance, or in the form of an emulsion or microemulsion (in particular an oil-in-water (O / W) or water-in-oil (W / O) type, a silicone-in-water (Si / W) or a water-in-silicone (W / Si) type, a PIT emulsion, a multiple emulsion (for example an oil-in-water-in-oil (O / W / O) or a water-in-oil-in-water (W / O / W) type), a Pickering emulsion, a hydrogel, an alcohol gel, a lipogel, a single-phase or multi-phase solution or a vesicular dispersion or other commonly used forms, which may also be applied with a pen-type device as a mask or spray.

[0059] The sunscreen composition according to the invention is advantageously in the form of an oil-in-water (O / W) emulsion comprising an oil phase dispersed in an aqueous phase in the presence of an O / W emulsifier. The preparation of such O / W emulsions is well known to those skilled in the art and is illustrated in the examples.

[0060] In an advantageous embodiment, the O / W emulsifier is a phosphate emulsifier. Among the preferred phosphate emulsifiers there is C 8-10 Alkyl ethyl phosphate, C 9-15 Alkyl phosphate, ceteareth-2 phosphate, ceteareth-5 phosphate, ceteth-8 phosphate, ceteth-10 phosphate, cetyl phosphate, C 6-10 Alkyl ether-4 phosphate, C 12-15 Alkyl ether-2 phosphate, C 12-15 The phosphate emulsifier according to the present invention is potassium cetyl phosphate, which can be used as K is commercially available from DSM Nutritional Products Ltd Kaiseraugst.

[0061] Other suitable O / W emulsifiers according to the present invention include PEG-30 dipolyhydroxystearate, PEG-4 dilaurate, PEG-8 dioleate, PEG-40 sorbitan monooleate, PEG-7 glyceryl cocoate, PEG-20 almond glycerides, PEG-25 hydrogenated castor oil, glyceryl stearate (and) PEG-100 stearate, PEG-7 palm kernelate, PEG-8 oleate, PEG-8 laurate, PEG-60 almond glycerides, PEG-20 methyl glucose sesquistearate, PEG-40 stearate, PEG-100 stearate, PEG-80 Sorbitan laurate, steareth-2, steareth-12, oleth-2, ceteth-2, laureth-4, oleth-10, oleth-10 / PEG-10 oleyl ether, ceteth-10, isosteareth-20, ceteareth-20, oleth-20, steareth-20, steareth-21, ceteth-20, isoceteth-20, laureth-23, steareth-100, glyceryl stearate citrate, glyceryl stearate (self-emulsifying), stearic acid, stearic acid salts, polyglyceryl-3-methylglucose distearate. Other suitable emulsifiers are sorbitan oleate, sorbitan sesquioleate, sorbitan isostearate, sorbitan trioleate, lauryl glucoside, decyl glucoside, sodium stearoyl glutamate, sucrose polystearate and hydrated polyisobutylene. In addition, one or more synthetic polymers can be used as emulsifiers. For example, PVP eicosene copolymer, acrylate / C 10-30 Alkyl acrylate crosspolymer, acrylates / steareth-20 methacrylate copolymer, PEG-22 / dodecyl glycol copolymer, PEG-45 / dodecyl glycol copolymer, and mixtures thereof.

[0062] Another particularly suitable class of O / W emulsifiers are the nonionic self-emulsifying systems derived from olive oil, such as the ones known as (INCI name) cetyl olive oil and sorbitan olivate (chemical composition: sorbitan esters and cetearyl esters of olive oil fatty acids), sold under the trade name OLIVEM 1000.

[0063] Also suitable are commercially available polymeric emulsifiers, such as hydrophobically modified polyacrylic acids, for example acrylates / C10-30 alkyl acrylate crosspolymers, which are available under the trade name TR-1 and TR-2 are commercially available from Noveon.

[0064] Another particularly suitable class of emulsifiers are polyglycerol esters or fatty acid diesters, also known as polyglycerol esters / diesters (i.e. polymers in which one or more fatty acids are bound to polyglycerol by esterification), commercially available, for example, from Evonik as Isolan GPS [INCI name Polyglyceryl-4 diisostearate / polyhydroxystearate / sebacate (i.e. a diester of a mixture of isostearic acid, polyhydroxystearic acid and sebacic acid with polyglyceryl-4)] or from Cognis as Dehymuls PGPH (INCI Polyglyceryl-2 dipolyhydroxystearate).

[0065] Likewise suitable are polyalkylene glycol ethers, for example Brij 72 (polyoxyethylene (2) stearyl ether) or Brij 721 (polyoxyethylene (21) stearyl ether, available for example from Croda).

[0066] Particularly advantageous O / W emulsifiers according to the invention are one or more of the following: polyglyceryl-3 methylglucose distearate, lauryl glucoside (and) polyglyceryl-2 dipolyhydroxystearate, glyceryl stearate citrate, sodium cetearyl sulfate, cetearyl glucoside; polyglyceryl-6 stearate (and) polyglyceryl-6 behenate, olive oil cetyl alcohol esters (and) sorbitan olive oil esters, rosemaryl alcohol esters; Cetearyl Alcohol (and) Behenyl Alcohol (and) Arachidyl Glucoside, Cetearyl Alcohol (and) Coco-Glucoside, Coco-Glucoside (and) Coconut Alcohol, PEG-100 Stearate (and) Glyceryl Stearate, Sodium Stearoyl Glutamate, Steareth-20, Steareth-21, Steareth-25, Steareth-2, Ceteareth-25 and Ceteareth-6 (all listed by their INCI names).

[0067] The most preferred O / W emulsifier according to the present invention is one or more of Ceteareth-6 (optionally blended with stearyl alcohol) and Ceteareth-25.

[0068] The at least one O / W or Si / W type emulsifier is preferably used in an amount of 0.5 to 10 wt.-%, such as specifically in the range of 0.5 to 5 wt.-%, such as most specifically in the range of 0.5 to 4 wt.-%, based on the total weight of the composition.

[0069] Suitable W / O emulsifiers or W / Si emulsifiers according to the invention are: polyglyceryl-2-dipolyhydroxystearate, PEG-30 dipolyhydroxystearate, cetyl dimethicone copolyol, polyglyceryl-3 diisostearate polyglyceryl esters of oleic acid / isostearate, polyglyceryl-6 hexaricinolate, polyglyceryl-4-oleate, polyglyceryl-4-oleate / PEG-8 propylene glycol cocoate, magnesium stearate, sodium stearate, potassium laurate, potassium ricinoleate, sodium cocoate, sodium tallowate, potassium castorate, sodium oleate, and mixtures thereof. Other suitable W / Si emulsifiers are lauryl polyglyceryl-3, polydimethylsiloxyethyl dimethicone and / or PEG-9 polydimethylsiloxyethyl dimethicone and / or cetyl PEG / PPG-10 / 1 dimethicone and / or PEG-12 dimethicone crosspolymer and / or PEG / PPG-18 / 18 dimethicone. A particularly suitable W / O emulsifier to be used in the composition according to the invention is PEG-30 dipolyhydroxystearate. The at least one W / O emulsifier is preferably used in an amount of about 0.001% to 10% by weight, more preferably 0.2% to 7% by weight, relative to the total weight of the composition.

[0070] In addition, the sunscreen composition according to the invention advantageously contains at least one co-surfactant, for example selected from the group consisting of mono- and di-glycerides and / or fatty alcohols. The co-surfactant is usually used in an amount selected from the range of 0.1% to 10% by weight, such as in particular the range of 0.5% to 7% by weight, such as most particularly the range of 1% to 5% by weight, based on the total weight of the composition. Particularly suitable co-surfactants are selected from the following list: alkyl alcohols, such as cetyl alcohol (Lorol C16, Lanette 16), cetearyl alcohol (Lanette O), stearyl alcohol (Lanette 18), behenyl alcohol (Lanette 22), glyceryl stearate, glyceryl myristate (Estol 3650), hydrogenated coconut oil glyceride (Lipocire Na 10) and mixtures thereof.

[0071] In a particularly advantageous embodiment according to the invention, the emulsifier is selected from the group consisting of Ceteareth-6 and / or Ceteareth-25, and the co-surfactant is selected from the group consisting of Behenyl Alcohol, Cetyl Alcohol, Cetearyl Alcohol and / or Stearyl Alcohol.

[0072] The composition in the form of an O / W emulsion according to the invention can be provided, for example, in all formulations of O / W emulsions, for example in the form of serums, emulsions or creams, and they are prepared according to the usual methods. The composition that is the subject of the invention is intended for topical application and can in particular constitute a dermatological or cosmetic composition, which is intended, for example, for protecting human skin from the adverse effects of ultraviolet radiation (anti-wrinkle, anti-aging, moisturizing, sun protection, etc.).

[0073] According to one advantageous embodiment of the invention, the composition constitutes a cosmetic composition and is intended to be applied topically to the skin.

[0074] In an advantageous aspect of the invention, the sunscreen composition according to the invention does not comprise (ie is free of) 3-(4-methylbenzylidene)-camphor and 2-hydroxy-4-methoxybenzophenone (INCI: oxybenzone).

[0075] In another advantageous aspect of the present invention, the sunscreen composition according to the present invention further comprises one or more of dibutyl adipate, dicaprylyl carbonate, stearyl alcohol, C12-C15 alkyl benzoate, caprylyl carbonate, capric / caprylic triglyceride and mixtures thereof, preferably dibutyl adipate, stearyl adipate and / or dicaprylyl carbonate.

[0076] In another advantageous aspect of the invention, the sunscreen composition according to the invention further comprises ethanol, more preferably in an amount of 0.5 to 10 wt.-%, more preferably 3 to 8 wt.-% ethanol, based on the total weight of the sunscreen composition.

[0077] In still another advantageous aspect of the present invention, the sunscreen composition of the present invention further comprises a preservative and / or a preservative synergist, preferably selected from the group consisting of phenoxyethanol, ethylhexylglycerin, hexylglycerin, glyceryl caprylate, caprylyl glycol, 1,2-hexanediol, propylene glycol, propylene glycol, p-hydroxyacetophenone and mixtures thereof, most preferably selected from the group consisting of phenoxyethanol and ethylhexylglycerin and mixtures thereof. When present, the preservative or preservative synergist is preferably used in an amount of 0.01% to 2% by weight, more preferably in an amount of 0.05% to 1.5% by weight, most preferably in an amount of 0.1% to 1.0% by weight, for example 0.1% to 0.5% by weight, based on the total weight of the composition.

[0078] In another advantageous aspect, the sunscreen composition according to the invention does not contain any parabens, benzethonium chloride, piroctone olamine, lauroyl arginine, methylisothiazolinone, chloromethylisothiazolinone, bronopol, benzalkonium chloride, formaldehyde-releasing compounds, salicylic acid, triclosan, DMDM ​​hydantoin, chlorphenesin and IPBC (iodopropynyl butylcarbamate).

[0079] In still further advantageous aspects, the sunscreen composition of the present invention further comprises one or more of glycerin or polyhydroxystearic acid and mixtures thereof.

[0080] In yet still further advantageous aspects, the sunscreen composition of the present invention further comprises a thickener, preferably a gum (such as xanthan gum or Caesalpina spinosa gum), or a polyacrylate (such as polyacrylate crosspolymer-6) and mixtures thereof.

[0081] The pH of the sunscreen composition according to the present invention is generally in the range of 3 to 10, preferably in the range of pH 4 to 8, and most preferably in the range of pH 4 to 7. The pH can be easily adjusted as required with a suitable acid (e.g. citric acid) or base (e.g. NaOH) according to standard methods in the art.

[0082] The sunscreen composition according to the present invention may also contain one or more emollients that soothe and soften the skin. For example, the emollient may be dicaprylyl carbonate or dibutyl adipate. Other emollients are silicones (dimethicone, cyclomethicone), vegetable oils (grape seed oil, sesame seed oil, jojoba oil, etc.), butters (cocoa butter, shea butter), alcohols (stearyl alcohol, cetyl alcohol), and vaseline derivatives (petroleum jelly, mineral oil).

[0083] In still another aspect, the sunscreen composition of the present invention may include potassium cetyl phosphate as an emulsifier, and / or one or more of dibutyl adipate, dicaprylyl carbonate, and stearyl alcohol.

[0084] In yet another aspect, the sunscreen composition of the present invention may comprise an acrylate copolymer, such as acrylate / C 10-30 Alkyl acrylate crosspolymer, even more preferably, an acrylate copolymer in an amount of 0.05 to 1 wt%, more preferably 0.1 to 0.5 wt%, based on the total weight of the sunscreen composition.

[0085] In another aspect, the sunscreen composition of the present invention may comprise one or more fragrances selected from limonene, citral, linalool, α-isomethyl ionone, geraniol, citronellol, 2-isobutyl-4-hydroxy-4-methyltetrahydropyran, 2-tert-amylcyclohexyl acetate, 3-methyl-5-phenyl-1-pentanol, 7-acetyl-1,1,3,4,4,6-hexamethyltetralin, diester of adipate, cinnamaldehyde, amyl salicylate, α-amyl cinnamaldehyde, α-methyl ionone, butylphenyl methylpropional, cinnamaldehyde, amyl cinnamyl alcohol, anise alcohol, benzoin, Benzyl alcohol, benzyl benzoate, benzyl cinnamate, benzyl salicylate, bergamot essential oil, bitter orange oil, butylphenyl methylpropional, cardamom essential oil, cedrol, cinnamaldehyde, cinnamyl alcohol, methyl citronellyl crotonate, citrus oil, coumarin, diethyl succinate, ethyl linalool, eugenol, tree moss extract, oak moss extract, farnesol, guaiac wood oil, hexyl cinnamaldehyde, hexyl salicylate, hydroxycitronellal, lavender oil, lemon oil, linalyl acetate, tangerine peel oil, menthol PCA ester, methyl heptenone, nutmeg oil, rosemary oil, sweet orange oil, terpineol, tonka bean oil, triethyl citrate, vanillin.

[0086] In another aspect, the sunscreen compositions of the present invention may contain at least one salt of 2-phenylbenzimidazole-5-sulfonic acid.

[0087] In another aspect, the composition may have an SPF of at least 20, preferably at least 30, for example an SPF of 20-50 or 30-50.

[0088] The sunscreen compositions according to the invention are treated with surprisingly low total amounts of UV filters.

[0089] According to the invention, it is advantageous if the sunscreen composition is in the form of an emulsion or dispersion, preferably in the form of an emulsion, more preferably in the form of an O / W emulsion.

[0090] In the case where the sunscreen composition of the present invention is in the form of an O / W emulsion, the preparation advantageously comprises one or more O / W emulsifiers selected from the group consisting of glyceryl stearate citrate, glyceryl stearate (self-emulsifying), stearic acid, stearates, polyglyceryl-3-methylglucose distearate, sodium cetearyl sulfate, potassium cetyl phosphate, polyglyceryl-10 stearate and sodium stearyl glutamate.

[0091] Advantageously, according to the invention, these O / W emulsifiers of the invention may be present in the preparation in a concentration ranging from 0.001% to 10% by weight, preferably from 0.1% to 7% by weight, based on the total weight of the preparation.

[0092] According to the invention, it is preferred if the preparation comprises potassium cetyl phosphate as emulsifier.

[0093] According to the invention, it is further advantageous if the preparation comprises cetyl alcohol, stearyl alcohol and / or glyceryl stearate.

[0094] According to the invention, it is advantageous if the preparations according to the invention are free of polyethylene glycols, polyethylene glycol ethers and polyethylene glycol esters (so-called PEG derivatives).

[0095] The preparations according to the invention may advantageously comprise a humectant. A humectant is a compound or a mixture of compounds which imparts to the cosmetic preparation the quality of reducing water loss from the stratum corneum (also known as transepidermal water loss (TEWL)) and / or positively influencing hydration of the stratum corneum after application or distribution on the skin surface.

[0096] Non-limiting examples of advantageous humectants for use in the present invention include glycerol, lactic acid and / or lactates, in particular sodium lactate, butylene glycol, propylene glycol, biosaccharide gum-1, soya bean, ethylhexylglycerol, pyrrolidone carboxylic acid and urea. A further advantage is in particular the use of polymeric humectants from the group of polysaccharides that are water-soluble and / or swellable in water and / or gellable with the aid of water. Particularly advantageous are, for example, hyaluronic acid, chitosan and / or fucose-rich polysaccharides, which are registered in Chemical Abstracts under registration number 178463-23-5 and can be found, for example, under the name 1000 is available from SOLABIAS, A. Moisturizers can also be used advantageously as active anti-wrinkle ingredients to protect against the type of skin changes that occur, for example, with skin aging.

[0097] The cosmetic preparations according to the invention may advantageously (although not obligatory) further comprise fillers which have the effect, for example, of further improving the organoleptic and cosmetic properties of the preparations and of evoking or enhancing, for example, a velvety or silky skin feel. Advantageous fillers in the sense of the present invention are starch and starch derivatives (for example tapioca starch, distarch phosphate, aluminum starch octenylsuccinate or sodium starch octenylsuccinate, etc.), pigments which have neither a substantial UV filter effect nor a coloring effect (for example boron nitride, etc.) and / or (CAS No. 7631-86-9) and / or talc and / or polyethylene, nylon and silica dimethyl silylate.

[0098] Advantageous embodiments of the preparation according to the invention also include those in which the preparation comprises one or more oils selected from the group consisting of butylene glycol dicaprylate / dicaprate, phenethyl benzoate, C 12-15Alkyl benzoate, dibutyl adipate, diisopropyl sebacate, dicaprylyl carbonate, di-C tartrate 12-13 Alkyl esters, butyl octyl salicylate, diethylhexyl syringylidenemalonate, hydrogenated castor oil dimerates, triheptanoin, lactic acid C 12-13 Alkyl esters, benzoic acid C 16-17 Alkyl Esters, Propylheptyl Caprylate, Caprylic / Capric Triglyceride, Diethylhexyl 2,6-Naphthalene Dicarboxylate, Octyldodecanol, Ethylhexyl Cocoate.

[0099] According to the invention, if the preparation contains dibutyl adipate, dicaprylyl carbonate and / or C benzoate 12-C15 Alkyl esters are preferred.

[0100] The aqueous phase of the preparation according to the invention can advantageously contain customary cosmetic adjuvants, for example alcohols, in particular low-carbon alcohols, preferably ethanol and / or isopropanol, or low-carbon polyols, and their ethers, preferably propylene glycol, glycerol; electrolytes; self-tanning agents; and in particular one or more thickeners, which can advantageously be selected from the following group: silicon dioxide, aluminum silicate, polysaccharides and / or their derivatives, for example hyaluronic acid, xanthan gum, hydroxypropyl methylcellulose, particularly advantageously from the following group: polyacrylates, preferably from the group known as carbomers, examples being carbomers of types 980, 981, 1382, 2984 and 5984, in each case used alone or in combination. Other thickeners that are advantageous according to the invention are those with the INCI name Acrylates / C10-30 Alkyl Acrylate Crosspolymer (e.g. Pemulen TR 1, Pemulen TR 2, Carbomer 1328 from NOVEON) and also Aristoflex AVC (INCI: Ammonium Acryloyldimethyltaurate / VP Copolymer) and Simugel NS (INCI: Hydroxyethyl Acrylate / Sodium Acryloyldimethyltaurate Copolymer and Squalane and Polysorbate 60).

[0101] According to the invention, if the preparation comprises xanthan gum, cross-linked acrylate / acrylic acid C 10-C30 Alkyl ester polymers, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymers and squalane and polysorbate 60 and / or vinylpyrrolidone / hexadecene copolymers are preferred here.

[0102] According to the invention, an amount of glycerol of at least 2.5% by weight, based on the total weight of the preparation, is particularly advantageous.

[0103] According to the invention, it is advantageous if the preparation comprises one or more alkanediols selected from the group consisting of 1,2-pentanediol, 1,2-hexanediol, 1,2-octanediol, 1,2-decanediol, 2-methyl-1,3-propanediol.

[0104] According to the invention, it is advantageous if the preparation according to the invention comprises ethanol, phenoxyethanol and / or ethylhexylglycerol.

[0105] Advantageously, according to the invention, the preparation according to the invention comprises a film former. Film formers in the sense of the invention are substances in various forms and characterized by the following property: when the film former is dissolved in water or other suitable solvents, when the solution is subsequently applied to the skin, the film former forms a film after evaporation of the solvent, which film essentially serves to fix the UV filter on the skin and thus increase the SPF and / or water resistance of the product.

[0106] It is particularly advantageous to select the film former from the group of polymers based on polyvinylpyrrolidone (PVP).

[0107] Particularly preferred are copolymers of vinyl pyrrolidone such as, for example, PVP hexadecene copolymer and PVP eicosene copolymer, which are available from GAF Chemicals Corporation under the trade names Antaron V216 and Antaron V220.

[0108] Also advantageous are other polymeric film formers, such as sodium polystyrene sulfonate, which is available from National Starchand Chemical Corp. under the trade name Flexan 130; and / or polyisobutylene, which is available from Rewo under the trade name Rewopal PIB1000. Examples of other suitable polymers are polyacrylamide (Seppigel 305), polyvinyl alcohol, PVP, PVP / VA copolymer, polyethylene glycol, acrylate / octylacrylamide copolymer (Dermacryl 79). Also advantageous is the use of hydrogenated castor oil dimer dioleate (CAS 646054-62-8, INCI hydrogenated castor oil dimer dioleate), which is available from Kokyu Alcohol Kogyo under the name Risocast DA-H; or PPG-3 benzyl ether myristate (CAS 403517-45-3), which is available from Croda Chemicals under the trade name Crodamol STS.

[0109] The following examples are provided to further illustrate the compositions and effects of the present invention. These examples are merely illustrative and are not intended to limit the scope of the present invention in any way. Example

[0110] In vitro UV model

[0111] The microbial strains used in this study are listed below and their cultures were reconstituted according to the instructions of the supplier's reference strain collection CRBIP (Institut Pasteur Collection, France) or ATCC (American Type Culture Collection):

[0112] -Lactobacillus crispatus CIP 103604

[0113] -Staphylococcus epidermidis ATCC 12228

[0114] Fill culture medium (140 μ L) in 96 well plates, and add the calibrated inoculum (20 μ L) of every kind of bacterial strain to reach the ultimate concentration of 1.106 colony forming units / milliliter (cfu / mL).After contamination, add sample (40 μ L in triplicate; 20% v / v), and fully mix to ensure uniform distribution.Then, according to the UV dosage shown in the following table 1, plate is subjected to UV stress by UV irradiator system (BioSun, Vilber Lourmat, France).Selected specific UV dosage is for making the survival rate of microbial colony between 10% and 49%.

[0115] strain SED(unit) <![CDATA[UVA+UVB(mJ / cm 2 )]]> Survival rate after 4 hours (%) Staphylococcus epidermidis 15 150 ±* Lactobacillus crispatus 50 500 -

[0116] Table 1. Selection of UV irradiation conditions for each microbial strain. The energy ratio between UVA and UVB is 1:19. ±*: the survival rate of the microbial population is between 21% and 49%; -: the survival rate of the microbial population is equal to or lower than 20%.

[0117] For the microbial population counts, aliquots of contaminated samples of Lactobacillus crispatus and Staphylococcus epidermidis were serially diluted (4 ten-fold dilutions followed by 8 two-fold dilutions in triplicate) in a medium on a 96-well plate containing triphenyltetrazol. The plates were then incubated at 37°C ± 2,5°C during 48h, taking into account the respiratory type of each strain. After 48h under optimal growth conditions, the final dilution exhibited a pink color (triphenyltetrazol), or turbidity indicated the contamination rate and allowed the determination of the reduction rate of the microbial population (most probable number MPN method). The control performed during this experiment was a medium containing bacteria incubated without UV stress. The microbial population counts are expressed in cfu / mL and are the average of experiments performed in triplicate.

[0118] The use levels of single UV filters and combinations in the test formulations were calculated to obtain predicted in vitro SPF values ​​of 5.75 + / - 0.15 and 23.45 + / - 0.65, respectively (Table 2). The UV filters were mixed in two oils, Tegosoft XC (55%) and Miglyol 829 Eco (ad 100%), and the emulsifier Tween 80 (5%).

[0119] The effect of selected UV irradiation conditions on the survival of the microbial populations Lactobacillus crispatus and Staphylococcus epidermidis in the presence of 20% v / v of the test samples 1-10 was compared with the corresponding strains in the absence of samples 1-10 and without exposure to UV irradiation (control). The results after 4 hours of incubation were compared with the bacterial population incubated without UV stress (determination of bacterial population survival) and expressed as a percentage of survival. The relative protection percentage (%) was calculated based on the difference between the survival percentages of the sample and the vehicle control (with UV irradiation) divided by the survival percentage of the vehicle control. Therefore, the survival rate of each microbial population and the relative protection achieved by the UV filter were calculated and summarized in Table 2.

[0120] Table 2. Test formulations of UV filters and ingredients in % by weight (* mixed with 55% TegosoftXC, ad100% Miglyol 829Eco, 5% Tween 80). The survival results for each microbial population are calculated in % compared to the untreated and non-irradiated control. In brackets: the relative protection achieved by the UV filters is given in % relative to the vehicle control.

[0121]

[0122]

[0123]

[0124] In vivo study setup

[0125] This clinical single-blind placebo-controlled trial specifically assessed the effects of UV irradiation on the composition of the skin microbiome of human skin and the protection of the skin microbiome by a representative SFP20 sunscreen versus a placebo formulation.

[0126] The investigational product consisted of two test formulations, one containing four UV filters (i.e. Shield, 340, EHS and 1789) for broad spectrum UV protection, and a vehicle formulation without UV filters (“placebo”) to assess the potential to protect the skin microbiome from UV exposure:

[0127] 1. Placebo formulation, which contained vehicle only; the UV filter had been replaced by a higher oil content.

[0128] 2.SPF20 sunscreen formula

[0129] Table 3: List of ingredients for placebo formulation and SPF20 sunscreen formulation

[0130]

[0131]

[0132] After obtaining informed consent from the volunteers, the test formulation was applied to the backs of 10 healthy female subjects (22-43 years old, Fitzpatrick skin type II-III), followed by two minimal erythema doses (2 MED) of UV irradiation. 2 Approximately 2 mg of both test formulations were applied to approximately 25 cm of the body surface. 2 Two test areas of skin. The individual MED of the subjects was determined one day before treatment. Prior to UV irradiation, skin surface swabs were taken from untreated, non-irradiated areas for analysis. Three defined areas on the back (approximately 25 cm 2 ) were exposed to 2 MED of UV radiation using a single-port solar simulator. Two hours after UV exposure, skin swab samples were collected on the three irradiated areas for analysis.

[0133] DNA was extracted from swab samples of nine completers using the ZymoBIOMICS DNA Miniprep kit according to the manufacturer's protocol and sequenced on an Illumina MiSeq in a 2×300 paired configuration. Primers targeted the V3-V4 region of the bacterial 16S rRNA gene. Sequences were then processed into amplicon sequence variants (ASVs).

[0134] A software tool called Songbird, as listed in Morton et al. (Nat. Commun., 2019.10(1): p. 2719), was applied to the categorical variables of radiation, treatment, and verum to study the differential ranking of taxonomy from the perspective of 16S rDNA. Songbird models the differential ranking to provide the relationship between each feature and the classification or quantitative measurement in question. These different features then contain coefficients to describe their relationship with the modeled variables.

[0135] In vivo results:

[0136] Ten Caucasian female subjects aged between 22 and 43 years (mean age 32±2 years, Fitzpatrick skin types II (4 subjects) and III (6 subjects)) were enrolled and completed the study. No adverse reactions were observed during the study.

[0137] Based on 16S rRNA sequencing analysis of samples from nine completers, Lactobacillus crispatus was identified as positively associated only with the formulation containing UV filters, whereas it showed negative associations with irradiation and with the placebo formulation.

[0138] Table 4: Coefficients for two Lactobacillus crispatus species from Songbird. T:YES is the association of these traits with verum, compared to T:NO which is the control for this variable.

[0139]

[0140] Specific microorganisms will benefit from additional UV protection, as observed with Lactobacillus crispatus. Interestingly, Lactobacillus crispatus is rarely described as a skin commensal. These major vaginal bacteria play a key role by forming beneficial biofilms to protect host tissues and act as components of the innate immune response. They metabolize glucose to lactic acid, thereby creating an acidic environment (~ pH 4-4.5), competitive exclusion, and release antimicrobial compounds (e.g., H2O2, bacteriocins, and other byproducts), thereby maintaining the skin's self-defense and the symbiosis of the skin microbial flora.

Claims

1. A composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone for use in treating or preventing dysbiosis of the skin and / or for strengthening the surface barrier of the skin after UV radiation.

2. A composition according to claim 1 for treating or preventing dysbiosis of the skin and / or strengthening the surface barrier of the skin after UV radiation by protecting bacteria of the genus Lactobacillus, preferably Lactobacillus crispatus and Staphylococcus epidermidis, from the harmful effects of UV radiation, wherein the Lactobacillus, preferably Lactobacillus crispatus and Staphylococcus epidermidis are present on the external surfaces of the human body with a skin microbiota containing said bacteria.

3. Composition according to claim 1 and / or 2, wherein the protection of said bacteria of the genus Lactobacillus is characterized in that after exposure to UV radiation said survival rate is increased by at least 100% compared to a control.

4. The composition according to any one or more of claims 1 to 3, wherein the protection against the Staphylococcus epidermidis is characterized in that after exposure to UV radiation the survival rate is increased by at least 20% compared to a control.

5. Composition according to any one or more of claims 1 to 4, wherein the amount of the one or more UV filters in the composition is selected such that the composition exhibits an SPF of at least 20.

6. Composition according to any one or more of claims 1 to 5, wherein the skin bacterium of the genus Lactobacillus is Lactobacillus crispatus.

7. Composition according to any one or more of claims 1 to 6, wherein the skin bacterium of the genus Lactobacillus is Lactobacillus crispatus CIP 103604.

8. The composition according to any one or more of claims 1 to 7, wherein the Staphylococcus epidermidis is Staphylococcus epidermidis ATCC 12228.

9. The composition according to any one or more of claims 1 to 8, wherein the amount of ethylhexyl triazone in the composition is selected from the range of 0.1 wt.-% to 10 wt.-%, preferably from the range of 0.5 wt.-% to 7.5 wt.-%, most preferably from the range of 1 wt.-% to 5 wt.-%, based on the total weight of the composition.

10. The composition according to any one or more of claims 1 to 9, wherein the amount of butyl methoxydibenzoylmethane in the composition is selected from the range of 0.1% to 10% by weight, preferably from 0.5% to 7.5% by weight, most preferably from 1% to 5% by weight, based on the total weight of the sunscreen composition.

11. The composition according to any one or more of claims 1 to 10, wherein the amount of octocrylene in the composition is selected from the range of 0.1 wt.% to 10 wt.%, more preferably from 0.5 wt.% to 7.5 wt.%, most preferably from 1 wt.% to 5 wt.%, based on the total weight of the composition.

12. A method for protecting bacteria of the genus Lactobacillus and Staphylococcus epidermidis from the harmful effects of UV radiation, the method comprising the step of applying to an external surface of a human body having a skin microbiome comprising said bacteria, prior to exposing said external surface to UV radiation, an effective amount of a sunscreen composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane, ethylhexyl triazone and zinc oxide.

13. A method of increasing the differential abundance of bacteria of the genus Lactobacillus, preferably Lactobacillus crispatus, in the skin microbiome of an individual after exposure to UV radiation, the method comprising the step of topically applying to an external surface of a body in need thereof, prior to exposing the external surface to UV radiation, an effective amount of a composition comprising one or more UV filters selected from the group consisting of octocrylene, butyl methoxydibenzoylmethane and ethylhexyl triazone.

14. The method of claim 13, wherein the composition comprises octocrylene, ethylhexyl salicylate, bis-ethylhexyloxyphenol methoxyphenyl triazine, and butyl methoxydibenzoylmethane.

15. The method according to claim 14, wherein the total amount of the UV filter is selected from the range of 15-20 wt% based on the total weight of the composition.

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