Attractant for luring Necrobia, luring core and application

Abstract

The invention relates to the technical field of forestry pest prevention and control, in particular to an attractant for attracting Necrobia, a lure and application. The attractant is prepared from the following components in parts by weight: 0.02 to 0.16 part of bark beetle dienol and 1 to 8 parts of volatile matter; the volatile matter is prepared from one or more of cis-3-hexenyl acetate, alpha-pinene, beta-pinene, 3-carene, limonene and turpentine oil; the Necrobia comprises Necrobia rufipes and / or Necrobia termiform. The adult Necrobia rufigena and Necrobia termite attractant provided by the invention provides a new thought and method for attracting predatory natural enemies to prevent and control the harm of bark beetles and obtaining natural enemy provenance. The lasting period of the attractant can reach 90 days, and the attractant has important significance for realizing attraction of natural enemy insects and prevention and treatment of bark beetle harm, is environment-friendly, reduces the use of chemical pesticides, is particularly suitable for popularization and application in forests, and has good economic and ecological benefits.

Description

Technical Field

[0001] The invention relates to the technical field of forestry harmful organism control, in particular to an attractant for attracting worms, an attractant core and application thereof. Background Art

[0002] The bark beetle is the most harmful forest pest in the coniferous forest ecosystem worldwide. There are many natural enemies of the bark beetle, which are biological factors that naturally regulate the bark beetle population and are of great significance for the biological control of the bark beetle. Among the many natural enemy insects, the most common one with very strong predation ability is the bark beetle. At present, there are many species of bark beetles that can prey on bark beetles, including red-breasted bark beetle Thanasimus substriatus, ant-shaped bark beetle Thanasimus formicarius, and mountain bark beetle Thanamicus dubius (Fang et al., 2021; Ye Hui et al., 1998; 2006; Ma Jianyuan et al., 2022). These species have become important biological control resources for studying the interaction between bark beetles and bark beetles, bark beetle control, and bark beetle breeding and application.

[0003] The attraction and breeding of natural enemy insects first requires corresponding attracting technology to obtain sufficient insect sources, but the technology that can effectively attract Guogong insects is still in the initial development stage. A large number of studies have shown that the aggregation pheromone of bark beetles has a strong attraction to various Guogong insects (Renwick et al., 1977; Yuan Fei et al., 2012). Cheng Bin et al. (2022) used a formula of 1.5-150 parts of 2-methyl-3-butene-2-ol, 0.01-60 parts of cis-verbenol, and 0.01-60 parts of tooth bark beetle alcohol to attract red-breasted Guogong insects, and used it as an attractant for the Guogong insect. Fang et al. (2021) showed that the combination of only 2-methyl-3-butene-2-ol and cis-verbenol can also attract red-breasted Guogong insects, and the host volatile α-pinene has a very strong synergistic effect on this attractant combination. However, from the current research reports, it is known that many bark beetles have obvious behavioral responses to sphenanthene, such as larch eight-toothed bark beetle Ips subelongatus, southern pine bark beetle Ips grandicollis, and coarse-toothed bark beetle Ips calligraphus. Therefore, based on sphenanthene, a broad-spectrum attractant for guinea pigs can be developed. However, relying solely on sphenanthene, a single ingredient, has a poor attractant effect.

[0004] In summary, combining the core components of the aggregation pheromone of bark beetles with volatiles from plant sources is a strategy for screening highly effective attractants for bark beetles. Based on the above technical difficulties and practical application needs, it is very necessary to provide a method for attracting bark beetles, which are predatory natural enemies of bark beetles. To address this problem, this method provides an attractant for attracting predatory natural enemies of bark beetles (red-breasted bark beetles and ant-shaped bark beetles). Summary of the invention

[0005] In order to solve the above problems, the present invention provides an attractant, an attractant core and an application for attracting Guogong worms, which are used for collecting and attracting the insect sources of Guogong worms.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides an attractant for attracting guinea pigs, comprising the following components in parts by weight: 0.02 to 0.16 parts of styrax dienol and 1 to 8 parts of volatiles;

[0008] The volatile matter includes one or more of cis-3-hexenyl acetate, α-pinene, β-pinene, 3-carene, limonene and turpentine;

[0009] The guogong worms include red-breasted guogong worms and / or ant-shaped guogong worms.

[0010] Preferably, the weight proportion of the scutellaria dienol is 0.04 to 0.12 parts, and the weight proportion of the volatile matter is 2 to 6 parts.

[0011] Preferably, the weight portion of the beetle dienol is 0.04 parts, and the weight portion of the volatile matter is 4 parts.

[0012] The present invention also provides the use of the attractant described in the above technical solution in attracting guogong insects, and the guogong insects include red-breasted guogong insects and / or ant-shaped guogong insects.

[0013] The present invention also provides an attractant core for attracting Guogong insects, comprising a slow-release carrier, absorbent cotton placed inside the slow-release carrier, and the attractant described in the above technical solution adsorbed on the absorbent cotton.

[0014] Preferably, the content of dienol in each lure is 20-160 mg, and the content of volatiles is 1-8 g.

[0015] Preferably, the content of sphenanthene alcohol in each lure is 40 mg, and the content of volatile matter is 4 g.

[0016] Preferably, the sustained-release carrier comprises a polyethylene plastic bag with a thickness of 22 filaments, a length of 150 mm, and a width of 50 mm;

[0017] The absorbent cotton is sterile absorbent cotton, and the content of the sterile absorbent cotton in each lure core is 3g.

[0018] The present invention also provides the use of the attractant core described in the above technical solution in luring Guogong insects, and the Guogong insects include red-breasted Guogong insects and / or ant-shaped Guogong insects.

[0019] Preferably, the application includes: hanging the lures in the woods, with 5 lures hung per acre.

[0020] Beneficial effects:

[0021] The attractant provided by the invention can attract red-breasted worms and ant-shaped worms, has excellent attracting effect, long lasting effect, good stability, environmental friendliness and the like, is suitable for attracting red-breasted worms and ant-shaped worms, and is not affected by terrain or topography.

[0022] The red-breasted guinea worm and ant-shaped guinea worm attractant provided by the present invention has clear ingredients, is easy to process and produce, has low cost, and can maintain a lasting effect of more than 90 days, which can meet the needs of actual production. The attractant of the present invention is an efficient and lasting guinea worm attractant, and the invention is an environmentally friendly green biotechnology that can be used to attract red-breasted guinea worm and ant-shaped guinea worm to prevent and control bark beetles, thereby achieving the effect of protecting coniferous forests. Secondly, a large number of red-breasted guinea worm and ant-shaped guinea worm insect sources can be obtained in combination with the trap, providing sufficient insect sources for the breeding of guinea worms. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0024] Figure 1 These are the results of behavioral tests of multiple volatiles and scutellaria dienol on two species of guogong insects in May 2023;

[0025] Figure 2 This is a test on the attractant effect of scutellaria dienol and cis-3-hexenyl acetate on two species of Guogong insects in June 2023;

[0026] Figure 3 This is a test on the attractant effect of different doses of scutellaria dienol on two species of guogong insects in June 2023;

[0027] Figure 4 This is a test on the attractant effect of different doses of cis-3-hexenyl acetate on two species of Guogong insects in June 2023;

[0028] Figure 5 It is the effective period of attracting dienol and cis-3-hexenyl acetate to two species of Gomphothera japonica from May to August 2024. DETAILED DESCRIPTION

[0029] The present invention provides an attractant for attracting gugong insects, comprising the following components in parts by weight: 0.02-0.16 parts of beetle dienol and 1-8 parts of volatiles; the volatiles include one or more of cis-3-hexenyl acetate, α-pinene, β-pinene, 3-carene, limonene and turpentine; the gugong insects include red-breasted gugong insects and / or ant-shaped gugong insects. In the present invention, the weight of the beetle dienol is preferably 0.04-0.12 parts, and the weight of the volatiles is preferably 2-6 parts. In the present invention, the weight of the beetle dienol is preferably 0.04 parts, and the weight of the cis-3-hexenyl acetate is preferably 4 parts. The present invention does not specifically limit the source of the above reagents, and commercially available products can be used. In the present invention, the commercially available styraxadienol (purity>95%, CAS:14434-41-4, Shanghai Youde Agricultural Technology Co., Ltd.), the purity of cis-3-hexenyl acetate>95% (CAS:3681-71-8, Shanghai Yuanye Biotechnology Co., Ltd.), the purity of α-pinene>95% (CAS:80-56-8, Shanghai Mairui Biochemical Technology Co., Ltd.), the purity of β-pinene is 98% (CAS:18172-67-3, McLean), the purity of 3-carene>95% (CAS:13466-78-9, McLean), the purity of limonene>95% (CAS:138-86-3, McLean), and the turpentine oil (a mixture of various terpenes, CAS:8006-64-2, Shanghai Jizhi Biochemical Technology Co., Ltd.).

[0030] The present invention also provides the use of the attractant described in the above technical solution in attracting guogong insects, and the guogong insects include red-breasted guogong insects and / or ant-shaped guogong insects.

[0031] The present invention also provides a lure for attracting the worm, comprising a slow-release carrier, a cotton wool placed inside the slow-release carrier, and the attractant according to the above technical solution adsorbed on the cotton wool. In the present invention, the content of scutellaria dienol in each lure is preferably 20 to 160 mg, and the content of volatiles is preferably 1 to 8 g. In the present invention, the content of scutellaria dienol in each lure is preferably 40 mg, and the content of volatiles is preferably 4 g.

[0032] In the present invention, the slow-release carrier preferably comprises a polyethylene plastic bag with a thickness of 22 threads, a length of 150 mm and a width of 50 mm. In the present invention, the absorbent cotton is preferably sterile absorbent cotton, and the content of sterile absorbent cotton in each lure is preferably 3 g. In the present invention, the polyethylene plastic bag contains 0.4% (W%) of antioxidant 168, 0.6% (W%) of dioctyl biphenyl dicarboxylate, and 99% (W%) of low-density polyethylene. In the present invention, an ultraviolet light adsorbent is preferably added to each lure, which is 10 mg of 2-hydroxy-4-methoxybenzophenone. The present invention has no special requirements on the source of sterile absorbent cotton and polyethylene plastic bag, and commercially available products can be used. The present invention has no special restrictions on the preparation method of the lure, and preferably includes the following steps: placing absorbent cotton in a slow-release carrier, then dripping the attractant on the sterile absorbent cotton, and sealing to obtain the lure. In the present invention, when the attractant also contains 2-hydroxy-4-methoxybenzophenone, the 2-hydroxy-4-methoxybenzophenone, beetle dienol and volatiles are mixed and then dropped onto absorbent cotton, and then sealed with a polyethylene plastic bag to obtain the attractant.

[0033] The present invention also provides the use of the attractant core described in the above technical solution in luring guogong insects, and the guogong insects include red-breasted guogong insects and / or ant-shaped guogong insects. The guogong insect attractant core provided by the present invention can be used to attract red-breasted guogong insects and ant-shaped guogong insects in large quantities. The guogong insect attractant core provided by the present invention is easy to process and produce, has low cost, and the two components are released stably on the carrier, and the effective period can be maintained at about 90 days, which can meet the needs of attracting red-breasted guogong insects and ant-shaped guogong insects during the entire flying period of the pest bark beetle. The attractant core provided by the present invention is an efficient, long-lasting, and environmentally friendly guogong insect attractant core, which can increase the number of red-breasted guogong insects and ant-shaped guogong insects trapped, and can realize the collection of the insect sources of the two types of guogong insects. The guogong insect attractant core of the present invention has broad industrialization prospects.

[0034] In the present invention, the lure is preferably hung in the woods during the application, and the number of hangings per acre is preferably 5. In a specific embodiment of the present invention, the lure is preferably fixed in a trap, and then the trap is hung in the woods. The trap is mainly a funnel-shaped trap, purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The trumpet connected to the insect collecting bottle is put on the lower part of the funnel and connected to the insect collecting bottle. Hang a lure in the middle of the top of the trap. The trap is hung in the woods, and the insect collecting bottle of the trap is preferably 50cm above the ground. The interval between the traps is 30 to 50m. Check and collect the trapped Guogong insects once every 2-3 days.

[0035] In order to further illustrate the present invention, the present invention is described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0036] Example 1

[0037] Results of behavioral tests on two species of Gomphothera indica using multiple volatiles and styrax dienol.

[0038] The Guogong insect attractants prepared in this embodiment all use commercially available scutellaria dienol, and its chemical purity and isomeric purity are both greater than 95%; commercially available α-pinene, β-pinene, 3-carene, limonene, turpentine, and cis-3-hexenyl acetate are used, and their chemical purity and isomeric purity are both greater than 95%.

[0039] The following five treatments were prepared according to test requirements.

[0040] Treatment 1: 40 mg of scutellariae;

[0041] Treatment 2: 40 mg of scutellariae; 4 g of α-pinene

[0042] Treatment 3: 40 mg of scutellariae dienol and 4 g of β-pinene;

[0043] Treatment 4: 40 mg of scutellariae dienol, 4 g of 3-carene;

[0044] Treatment 5: 40 mg of scutellariae, 4 g of limonene;

[0045] Treatment 6: 40 mg of scutellariae dienol, 4 g of turpentine;

[0046] Treatment 7: 40 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0047] (1) Prepare beetle dienol, α-pinene, β-pinene, 3-carene, limonene, turpentine and cis-3-hexenyl acetate according to the above ratios to obtain attractants with different ratios.

[0048] (2) Preparation and storage of bait core: Add the attractant to 3 g of sterile cotton wool and place it in a black polyethylene plastic bag for 20 seconds. The bait core is sealed with aluminum foil and refrigerated at 4°C.

[0049] (3) Field application of lure core:

[0050] a) Experimental location: Xianmi Forest Farm, Qinghai Province.

[0051] b) Production of the trap: The trap is mainly a funnel-shaped trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), which is connected to the upper cover through the hook at the top and to the funnel through the hook at the bottom. The horn connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0052] c) Suspension of bait core: Hang a bait core in the middle of the funnel-shaped trap, 20 cm away from the top.

[0053] d) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long and 5cm diameter plastic poles were inserted into the soil layer for about 50cm, with a distance of 30cm between the two plastic poles. Then the trap was fixed between the two plastic poles, and the insect collection bottle of the trap was 30cm above the ground.

[0054] e) Data collection and trap emptying: In this experiment, each treatment was repeated three times, and the number of worms trapped in each treatment was the total number of 3 traps. Depending on the weather conditions, the number of worms in the insect collection bottle was observed every 2-3 days. After counting, the trapped worms were placed in a plastic box and brought to the laboratory.

[0055] According to the above specific usage steps, it is used to lure red-breasted guinea pigs and ant-shaped guinea pigs. From May 17 to June 2, 2022, the trap with a blank lure core (treatment 1) was used as a blank control, and the trap with guinea pig attractant (treatment 2) was used as a positive control. The specific results are shown in Figure 1 The distance between traps was 30m, and the insect collecting bottle was 30cm above the ground. In this experiment, each treatment was repeated 3 times, and the number of Guogong insects trapped in each treatment was the average number of three traps. Depending on the weather conditions, the number of Guogong insects in the insect collecting bottle was observed every 2-3 days.

[0056] from Figure 1 It can be concluded that the analysis of the 15-day attractant effect found that the use of scutellaria dienol alone (treatment 1) has attractant activity for both red-breasted and ant-shaped guogong insects. However, when scutellaria dienol was used in combination with volatile substances α-pinene, β-pinene, 3-carene, limonene, turpentine, and cis-3-hexenyl acetate, the capture of both types of guogong insects increased. Among them, the combined use of cis-3-hexenyl acetate and scutellaria dienol captured the largest number of guogong insects. This experiment shows that scutellaria dienol and cis-3-hexenyl acetate can attract red-breasted and ant-shaped guogong insects in large quantities.

[0057] Example 2

[0058] Experiment on the attractant effect of scutellaria dienol and cis-3-hexenyl acetate on two species of scolopides.

[0059] The Guogong insect attractants prepared in this embodiment use commercially available scutellaria dienol, whose chemical purity and isomeric purity are both greater than 95%; and use commercially available cis-3-hexenyl acetate, whose chemical purity and isomeric purity are both greater than 95%.

[0060] The following five treatments were prepared according to test requirements.

[0061] Treatment 1: blank treatment, the lure was only sterile cotton wool;

[0062] Treatment 2: 40 mg of scutellariae;

[0063] Treatment 3: cis-3-hexenyl acetate 4 g;

[0064] Treatment 4: 40 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0065] (1) Prepare the beetle dienol and cis-3-hexenyl acetate according to the above ratio to obtain attractants with different ratios.

[0066] (2) Preparation and storage of bait core: Add the attractant to 3 g of sterile cotton wool and place it in a black polyethylene plastic bag for 20 seconds. The bait core is sealed with aluminum foil and refrigerated at 4°C.

[0067] (3) Field application of lure core:

[0068] a) Experimental location: Xianmi Forest Farm, Qinghai Province.

[0069] b) Production of the trap: The trap is mainly a funnel-shaped trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), which is connected to the upper cover through the hook at the top and to the funnel through the hook at the bottom. The horn connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0070] c) Suspension of bait core: Hang a bait core in the middle of the funnel-shaped trap, 20 cm away from the top.

[0071] d) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long and 5cm diameter plastic poles were inserted into the soil layer for about 50cm, with a distance of 30cm between the two plastic poles. Then the trap was fixed between the two plastic poles, and the insect collection bottle of the trap was 30cm above the ground.

[0072] e) Data collection and trap emptying: In this experiment, each treatment was repeated three times, and the number of worms trapped in each treatment was the total number of 3 traps. Depending on the weather conditions, the number of worms in the insect collection bottle was observed every 2-3 days. After counting, the trapped worms were placed in a plastic box and brought to the laboratory.

[0073] According to the above specific usage steps, it is used to lure red-breasted guinea pigs and ant-shaped guinea pigs. From June 2 to June 18, 2022, the trap with a blank lure core (treatment 1) was used as a blank control, and the trap with guinea pig attractant (treatment 2) was used as a positive control. The specific results are shown in Figure 2The distance between traps was 30m, and the insect collecting bottle was 30cm above the ground. In this experiment, each treatment was repeated 3 times, and the number of Guogong insects trapped in each treatment was the average number of three traps. Depending on the weather conditions, the number of Guogong insects in the insect collecting bottle was observed every 2-3 days.

[0074] from Figure 2 It can be concluded that from the 15-day insect attractant effect analysis, the Guogong insect attractant of the present invention (treatment 4) has a good attractant effect during the entire investigation period (June 2 to June 18). First, the blank treatment (treatment 1) has no attractant effect on red-breasted Guogong insects and ant-shaped Guogong insects. The use of small beetle dienol (treatment 2) and cis-3-hexenyl acetate (treatment 3) alone has attractant activity for red-breasted Guogong insects and ant-shaped Guogong insects. When the two are used in combination, the capture amount of red-breasted Guogong insects and ant-shaped Guogong insects increases by 5 times and 7 times respectively. This experiment shows that only when small beetle dienol and cis-3-hexenyl acetate are used in combination can red-breasted Guogong insects and ant-shaped Guogong insects be attracted in large quantities.

[0075] Example 3

[0076] Experiment on the attractant effect of different doses of scutellaria dienol on two species of scolopax.

[0077] The Guogong insect attractants prepared in this embodiment use commercially available scutellaria dienol, whose chemical purity and isomeric purity are both greater than 95%; and use commercially available cis-3-hexenyl acetate, whose chemical purity and isomeric purity are both greater than 95%.

[0078] The following five treatments were prepared according to test requirements.

[0079] Treatment 1: 20 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0080] Treatment 2: 40 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0081] Treatment 3: 80 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0082] Treatment 4: 120 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0083] Treatment 4: 160 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0084] (1) Prepare the beetle dienol and cis-3-hexenyl acetate according to the above ratio to obtain attractants with different ratios.

[0085] (2) Preparation and storage of bait core: Add the attractant to 3 g of sterile cotton wool and place it in a black polyethylene plastic bag for 20 seconds. The bait core is sealed with aluminum foil and refrigerated at 4°C.

[0086] (3) Field application of lure core:

[0087] a) Experimental location: Xianmi Forest Farm, Qinghai Province.

[0088] b) Production of the trap: The trap is mainly a funnel-shaped trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), which is connected to the upper cover through the hook at the top and to the funnel through the hook at the bottom. The horn connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0089] c) Suspension of bait core: Hang a bait core in the middle of the funnel-shaped trap, 20 cm away from the top.

[0090] d) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long and 5cm diameter plastic poles were inserted into the soil layer for about 50cm, with a distance of 30cm between the two plastic poles. Then the trap was fixed between the two plastic poles, and the insect collection bottle of the trap was 30cm above the ground.

[0091] e) Data collection and trap emptying: In this experiment, each treatment was repeated three times, and the number of worms trapped in each treatment was the total number of 3 traps. Depending on the weather conditions, the number of worms in the insect collection bottle was observed every 2-3 days. After counting, the trapped worms were placed in a plastic box and brought to the laboratory.

[0092] According to the above specific usage steps, it is used to lure red-breasted guinea pigs and ant-shaped guinea pigs. From June 2 to June 18, 2022, the trap with a blank lure core (treatment 1) was used as a blank control, and the trap with guinea pig attractant (treatment 2) was used as a positive control. The specific results are shown in Figure 3 The distance between traps was 30m, and the insect collecting bottle was 30cm above the ground. In this experiment, each treatment was repeated 3 times, and the number of Guogong insects trapped in each treatment was the average number of three traps. Depending on the weather conditions, the number of Guogong insects in the insect collecting bottle was observed every 2-3 days.

[0093] from Figure 3 It can be concluded that from the analysis of insect attraction effect (such as Figure 3As shown in the figure, it can be seen that treatments 1 to 5 can attract red-breasted guogong worms and ant-shaped guogong worms. When different amounts of scutellaria dienol are used in combination with a fixed amount of cis-3-hexenyl acetate, it can be found that with the increase in the amount of scutellaria dienol used, the amount of trapping of red-breasted guogong worms and ant-shaped guogong worms gradually tends to be stable, among which treatment 2 significantly increases the amount of trapping of red-breasted guogong worms and ant-shaped guogong worms than treatment 1. When the dosage reaches 120 mg, the trapping amount reaches the highest, but from the perspective of luring effect and economic investment, 40 mg of scutellaria dienol can have a significant luring effect on red-breasted guogong worms and ant-shaped guogong worms in the wild.

[0094] Example 4

[0095] Experiment on the attractant effect of different doses of scutellaria dienol on two species of scolopax.

[0096] The Guogong insect attractants prepared in this embodiment use commercially available scutellaria dienol, whose chemical purity and isomeric purity are both greater than 95%; and use commercially available cis-3-hexenyl acetate, whose chemical purity and isomeric purity are both greater than 95%.

[0097] The following five treatments were prepared according to test requirements.

[0098] Treatment 1: 40 mg of scutellariae dienol, 1 g of cis-3-hexenyl acetate;

[0099] Treatment 2: 40 mg of scutellariae dienol, 2 g of cis-3-hexenyl acetate;

[0100] Treatment 3: 40 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0101] Treatment 4: 40 mg of scutellariae dienol, 6 g of cis-3-hexenyl acetate;

[0102] Treatment 4: 40 mg of scutellariae dienol, 8 g of cis-3-hexenyl acetate;

[0103] (1) Prepare the beetle dienol and cis-3-hexenyl acetate according to the above ratio to obtain attractants with different ratios.

[0104] (2) Preparation and storage of bait core: Add the attractant to 3 g of sterile cotton wool and place it in a black polyethylene plastic bag for 20 seconds. The bait core is sealed with aluminum foil and refrigerated at 4°C.

[0105] (3) Field application of lure core:

[0106] a) Experimental location: Xianmi Forest Farm, Qinghai Province.

[0107] b) Production of the trap: The trap is mainly a funnel-shaped trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), which is connected to the upper cover through the hook at the top and to the funnel through the hook at the bottom. The horn connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0108] c) Suspension of bait core: Hang a bait core in the middle of the funnel-shaped trap, 20 cm away from the top.

[0109] d) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long and 5cm diameter plastic poles were inserted into the soil layer for about 50cm, with a distance of 30cm between the two plastic poles. Then the trap was fixed between the two plastic poles, and the insect collection bottle of the trap was 30cm above the ground.

[0110] e) Data collection and trap emptying: In this experiment, each treatment was repeated three times, and the number of worms trapped in each treatment was the total number of 3 traps. Depending on the weather conditions, the number of worms in the insect collection bottle was observed every 2-3 days. After counting, the trapped worms were placed in a plastic box and brought to the laboratory.

[0111] According to the above specific usage steps, it is used to lure red-breasted guinea pigs and ant-shaped guinea pigs. From June 2 to June 18, 2022, the trap with a blank lure core (treatment 1) was used as a blank control, and the trap with guinea pig attractant (treatment 2) was used as a positive control. The specific results are shown in Figure 4 The distance between traps was 30m, and the insect collecting bottle was 30cm above the ground. In this experiment, each treatment was repeated 3 times, and the number of Guogong insects trapped in each treatment was the average number of three traps. Depending on the weather conditions, the number of Guogong insects in the insect collecting bottle was observed every 2-3 days.

[0112] from Figure 4 It can be concluded that from the analysis of insect attraction effect (such as Figure 4 As shown in the figure, it can be seen that treatments 1 to 5 can all lure red-breasted guogong worms and ant-shaped guogong worms. When different amounts of cis-3-hexenyl acetate are used in combination with a fixed amount of scutellaria dienol, it can be found that with the increase in the amount of cis-3-hexenyl acetate used, the trapping amount of red-breasted guogong worms and ant-shaped guogong worms changes, and treatment 2 significantly increases the trapping amount of red-breasted guogong worms and ant-shaped guogong worms than treatment 1. When the dosage of cis-3-hexenyl acetate reaches 4g (treatment 3), the trapping amount reaches the highest, and then the dosage increases but the trapping amount decreases. Therefore, from the perspective of the luring effect, 4g of cis-3-hexenyl acetate can have a significant luring effect on red-breasted guogong worms and ant-shaped guogong worms in the wild.

[0113] Example 5

[0114] The attractant duration of cis-3-hexenyl acetate and succinimidyl alcohol to two species of scolopax.

[0115] The Guogong insect attractants prepared in this embodiment use commercially available scutellaria dienol, whose chemical purity and isomeric purity are both greater than 95%; and use commercially available cis-3-hexenyl acetate, whose chemical purity and isomeric purity are both greater than 95%.

[0116] The following five treatments were prepared according to test requirements.

[0117] Treatment 1: 40 mg of scutellariae dienol, 1 g of cis-3-hexenyl acetate;

[0118] Treatment 2: 40 mg of scutellariae dienol, 2 g of cis-3-hexenyl acetate;

[0119] Treatment 3: 40 mg of scutellariae dienol, 4 g of cis-3-hexenyl acetate;

[0120] Treatment 4: 40 mg of scutellariae dienol, 6 g of cis-3-hexenyl acetate;

[0121] Treatment 4: 40 mg of scutellariae dienol, 8 g of cis-3-hexenyl acetate;

[0122] (1) Prepare the beetle dienol and cis-3-hexenyl acetate according to the above ratio to obtain attractants with different ratios.

[0123] (2) Preparation and storage of bait core: Add the attractant to 3 g of sterile cotton wool and place it in a black polyethylene plastic bag for 20 seconds. The bait core is sealed with aluminum foil and refrigerated at 4°C.

[0124] (3) Field application of lure core:

[0125] a) Experimental location: Xianmi Forest Farm, Qinghai Province.

[0126] b) Production of the trap: The trap is mainly a funnel-shaped trap purchased from Beijing Zhongjie Sifang Biotechnology Co., Ltd. The large plate and the small plate are crossed to form a cross shape (500 mm high and 300 mm wide), which is connected to the upper cover through the hook at the top and to the funnel through the hook at the bottom. The horn connected to the insect collection bottle is placed on the lower part of the funnel and connected to the insect collection bottle.

[0127] c) Suspension of bait core: Hang a bait core in the middle of the funnel-shaped trap, 20 cm away from the top.

[0128] d) Field setting of traps: Three replicates were set for each experimental treatment. The traps were set at the edge of the spruce forest, and the distance between the traps was about 30m. Two 2m long and 5cm diameter plastic poles were inserted into the soil layer for about 50cm, with a distance of 30cm between the two plastic poles. Then the trap was fixed between the two plastic poles, and the insect collection bottle of the trap was 30cm above the ground.

[0129] e) Data collection and trap emptying: In this experiment, each treatment was repeated three times, and the number of worms trapped in each treatment was the total number of 3 traps. Depending on the weather conditions, the number of worms in the insect collection bottle was observed every 2-3 days. After counting, the trapped worms were placed in a plastic box and brought to the laboratory.

[0130] According to the above specific usage steps, it is used to lure red-breasted guinea pigs and ant-shaped guinea pigs. From June 2 to June 18, 2022, the trap with a blank lure core (treatment 1) was used as a blank control, and the trap with guinea pig attractant (treatment 2) was used as a positive control. The specific results are shown in Figure 5 The distance between traps was 30m, and the insect collecting bottle was 30cm above the ground. In this experiment, each treatment was repeated 3 times, and the number of Guogong insects trapped in each treatment was the average number of three traps. Depending on the weather conditions, the number of Guogong insects in the insect collecting bottle was observed every 2-3 days.

[0131] from Figure 5 It can be concluded that, from the analysis of the insect attracting effect, the Guogong insect attractant (treatment 5) involved in the present invention has a good attracting effect in the entire investigation period (May 17 to August 30), and has a stable attracting effect on both red-breasted Guogong insects and ant-shaped Guogong insects, and the synergistic lasting effect period reaches more than 90 days. This experiment shows that the Guogong insect attractant prepared by scutellaria dienol and cis-3-hexenyl acetate has a long-term attraction to red-breasted Guogong insects and ant-shaped Guogong insects, indicating that the composition of scutellaria dienol and cis-3-hexenyl acetate can be used as a good attractant to attract red-breasted Guogong insects and ant-shaped Guogong insects, the natural enemies of bark beetles, and long-term attracting can not only reflect the occurrence dynamics of Guogong insects, but also promote the attraction of natural enemies and the collection of insect sources.

[0132] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An attractant for attracting worms, characterized in that: The invention comprises the following components in parts by weight: 0.02 to 0.16 parts of beetle dienol and 1 to 8 parts of volatile matter; The volatile matter includes one or more of cis-3-hexenyl acetate, α-pinene, β-pinene, 3-carene, limonene and turpentine; The guogong worms include red-breasted guogong worms and / or ant-shaped guogong worms.

2. The attractant according to claim 1, characterized in that The weight proportion of the scutellaria dienol is 0.04 to 0.12 parts, and the weight proportion of the volatile matter is 2 to 6 parts.

3. The attractant according to claim 1, characterized in that The weight portion of the beetle dienol is 0.04 parts, and the weight portion of the volatile matter is 4 parts.

4. Use of the attractant according to any one of claims 1 to 3 in attracting worms, wherein the worms include red-breasted worms and / or ant-shaped worms.

5. A bait core for attracting worms, characterized in that: The invention comprises a slow-release carrier, absorbent cotton placed inside the slow-release carrier and the attractant according to any one of claims 1 to 3 adsorbed on the absorbent cotton.

6. The lure according to claim 5, characterized in that: The content of dienol in each bait core is 20 to 160 mg, and the content of volatile matter is 1 to 8 g.

7. The lure according to claim 5 or 6, characterized in that: The content of dienol in each bait core is 40 mg, and the content of volatiles is 4 g.

8. The lure core according to claim 5, characterized in that: The sustained-release carrier comprises a polyethylene plastic bag with a thickness of 22 filaments, a length of 150 mm and a width of 50 mm; The absorbent cotton is sterile absorbent cotton, and the content of the sterile absorbent cotton in each lure core is 3g.

9. Use of the bait core according to any one of claims 5 to 8 in luring worms, wherein the worms include red-breasted worms and / or ant-shaped worms.

10. The use according to claim 9, characterized in that: The application includes: hanging the lure cores in the forest, with 5 lures hung per acre.

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