Blood fat reducing ointment as well as preparation method and application thereof

Abstract

The invention discloses a blood fat reducing ointment as well as a preparation method and application thereof, and relates to the technical field of foods. When the blood fat reducing ointment is prepared, the longan aril, the gynura procumbens and the kudzu are used as main raw materials, and the longan aril is directly decocted for 6-8 hours in a steamer over water after being crushed; gynura procumbens and kudzu vine are subjected to two times of ultrasonic heating extraction with different parameters through purified water, and finally the extract, the longan pulp paste, xylooligosaccharide and alginate oligosaccharide are stirred and mixed to be uniform according to the mass ratio of 10: (5-6): (0.5-1): (0.5-0.8) to obtain the blood fat reducing paste. The blood fat reducing ointment has a good blood fat reducing effect, is good in food safety, simple in preparation process and suitable for industrial large-scale production, and has good economic benefits.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and specifically relates to a blood lipid lowering ointment and a preparation method and application thereof. Background Art

[0002] With the development of social economy, people's living standards are getting higher and higher. Under the high-intensity life and work pressure, people do not control their diet and reduce outdoor sports. As a result, diseases such as "three highs", obesity, and hyperlipidemia have become the norm in society. If the above problems are not controlled for a long time, they will further cause coronary heart disease, myocardial infarction, cerebral thrombosis and other diseases, seriously affecting people's quality of life.

[0003] At present, statins and fibrates are mainly used clinically to control blood lipids, but these drugs have toxic and side effects such as liver damage and gastrointestinal damage. Studies have shown that many phytochemical components have good lipid-lowering effects and no toxic and side effects. Chinese patent CN 117981828A has developed a lipid-lowering health drink with blue indigo fruit extract, black fungus extract, and hawthorn extract as main raw materials, which has a good lipid-lowering effect. However, its preparation process is complicated, and the three raw materials need to be ultrasonically extracted, centrifuged, and concentrated twice, occupying a lot of production equipment and high production costs; at the same time, the dosage form is selected as a drink, which has a large dosage and is inconvenient to carry and store. In addition, in order to cope with the problem of poor stability during storage, potassium sorbate, a preservative, is added to the health drink. Therefore, it is urgent to develop a new lipid-lowering preparation to solve such problems. Summary of the invention

[0004] The purpose of the present invention is to provide a blood lipid lowering ointment and its preparation method and application in view of the deficiencies of the prior art. The method is simple in process and highly operable, wherein the longan meat is directly boiled in a water-bathed steamer for 6-8 hours after being crushed, thereby effectively retaining the effective ingredients in the longan meat; the flat chrysanthemum Panax notoginseng and kudzu vine are subjected to ultrasonic heating extraction twice with purified water, and the heat-sensitive components of the flat chrysanthemum Panax notoginseng and kudzu vine are retained by two phases of extraction with different parameters, and there is no problem of solvent residue; finally, the extract, longan meat paste, oligoxylose and brown algae oligosaccharide are stirred and mixed in a mass ratio of 10: 5-6: 0.5-1: 0.5-0.8 to obtain a blood lipid lowering ointment. The blood lipid lowering ointment has a good blood lipid lowering effect, and the longan meat, flat chrysanthemum Panax notoginseng and kudzu vine in the formula of this product are all food raw materials, and the edible safety is good.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] The first object of the present invention is to provide a method for preparing a blood lipid-lowering ointment, comprising the following steps:

[0007] S1. After cleaning and removing impurities from the longan pulp, the longan pulp was crushed through a 60-80 mesh sieve and boiled in a water-tight steamer for 6-8 hours to obtain a longan pulp paste;

[0008] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3-4, and crush them through a 40-60 mesh sieve; add 8-10 times the total weight of purified water, perform the first heating ultrasonic extraction, and collect the first filtrate; add 4-6 times the total weight of purified water to the remaining filter residue, perform the second heating ultrasonic extraction, and collect the second filtrate;

[0009] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.08-1.18 g / cm 3 , obtaining an extract; stirring and mixing the extract, longan pulp paste, xylooligosaccharide and brown algae oligosaccharide in a mass ratio of 10:5-6:0.5-1:0.5-0.8 to obtain a lipid-lowering ointment.

[0010] Longan meat refers to the dried fruit baked from fresh longan. It is soft and slightly sticky, in block shape, rich in glucose, sucrose and protein, a large amount of iron, potassium and other elements, and contains flavonoids, triterpenoid saponins and other ingredients. It has the effects of replenishing qi and blood, lowering blood lipids, and helps increase coronary blood flow. It has a certain preventive effect on hypertension and coronary atherosclerotic heart disease. After the longan meat is crushed, it is directly boiled in a water-steaming pot for 6-8 hours, which effectively retains the effective ingredients in the longan meat. At the same time, it also sterilizes the longan raw materials, which is conducive to improving the storage stability of the lipid-lowering ointment in the later stage and is not easy to deteriorate. Flat-lying chrysanthemum Panax notoginseng is a plant for both medicinal and edible purposes. It can be directly used as a salad. It is rich in organic calcium and is a green food for calcium supplementation for middle-aged and elderly people. It also contains rich organic acid components and a variety of active ingredients such as coumarins, flavonoids, benzofurans, polysaccharides, etc. It has good analgesic, hemostatic, anti-malarial, anti-inflammatory, hypoglycemic, hypolipidemic, anti-tumor and other pharmacological activities. Kudzu vine, also known as Pueraria root, contains active ingredients such as isoflavones, daidzein, and kudzuvine root. Regular consumption can lower blood sugar and blood lipids, and is especially effective in lowering hyperlipidemia with elevated cholesterol.

[0011] The present invention uses longan pulp, chrysanthemum and kudzu together, which has a synergistic effect of lowering blood lipids. The extract is concentrated to 1.08-1.18g / cm 3 When the extract is added, it has good fluidity, which is convenient for mixing evenly with xylooligosaccharides and brown algae oligosaccharides. Too high an extract concentration will lead to uneven mixing, while too low a concentration will affect the stability of the finished ointment. Xylooligosaccharides and brown algae oligosaccharides have good water solubility and stability. Studies have found that adding xylooligosaccharides or brown algae oligosaccharides to the extract can effectively improve the taste of the ointment, have a synergistic effect on slowing down the decline of total flavonoids, and improve the storage stability of the ointment.

[0012] Furthermore, the method further comprises the following steps: S4, packaging the blood lipid lowering ointment into glass bottles, with each bottle containing 20-100 g; sealing the glass bottle mouths with heating aluminum foil, and covering the bottle caps after sealing to obtain the blood lipid lowering ointment finished product.

[0013] In order to ensure the storage stability and portability of the ointment, the lipid-lowering ointment is further packaged into 20-100g bottles. The bottle mouth is sealed with aluminum foil, which has good barrier properties and can prevent external microorganisms from entering the ointment and affecting the quality of the ointment. At the same time, the small packaging specifications are adopted, which are convenient to carry and can ensure that the ointment can be consumed in a short period of time. While ensuring product quality, it can also avoid adding additional preservatives to bring burdens to health.

[0014] Furthermore, in S2, the temperature of the first heating ultrasonic extraction is 60-70°C, the ultrasonic frequency is 30-40KHz, and the time is 1-2h.

[0015] Furthermore, in S2, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, and the time is 1.5h.

[0016] Furthermore, in S2, the temperature of the second heating ultrasonic extraction is 80-90°C, the ultrasonic frequency is 50-70KHz, and the time is 1-2h.

[0017] Furthermore, in S2, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, and the time is 1.2h.

[0018] Purified water was used to perform ultrasonic heating extraction twice for Chrysanthemum Notoginseng and Pueraria lobata. The inventors found that by performing two staged extractions with different parameters, first extracting under low temperature and low ultrasonic frequency conditions, and then extracting under high temperature and high frequency conditions, the heat-sensitive components in Chrysanthemum Notoginseng and Pueraria lobata can be retained to the greatest extent. The protein content in the obtained lipid-lowering ointment reached 26.8%, and the total flavonoids content reached 5.4%. At the same time, there was no problem of solvent residue, and it was safer to eat.

[0019] Furthermore, in S2, the mass ratio of the extract, longan pulp paste, xylooligosaccharide, and brown algae oligosaccharide is 10:5.5:0.8:0.6. The inventors found that when the mass ratio of the extract, longan pulp paste, xylooligosaccharide, and brown algae oligosaccharide is 10:5.5:0.8:0.6, the synergistic effect of lowering blood lipids is better.

[0020] The second object of the present invention is to provide a blood lipid-lowering ointment prepared by the above preparation method.

[0021] The third object of the present invention is to provide the application of the above-mentioned lipid-lowering ointment in medicine and lipid-lowering functional food. The lipid-lowering ointment prepared by the present invention uses food raw materials as the main raw materials, does not need to add additional preservatives, has a good lipid-lowering effect, and is safer to eat.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. In the preparation of the lipid-lowering ointment, the present invention uses longan pulp, chrysanthemum notoginseng, and kudzu vine as the main raw materials, wherein the longan pulp is directly boiled in a water-steamer for 6-8 hours after being crushed, which effectively retains the effective ingredients in the longan pulp, and also sterilizes the longan raw materials, which is beneficial to improve the storage stability of the lipid-lowering ointment in the later stage, and is not easy to deteriorate; the chrysanthemum notoginseng and kudzu vine are extracted by ultrasonic heating twice with purified water, and the heat-sensitive components in the chrysanthemum notoginseng and kudzu vine can be retained to the greatest extent through two phased extractions with different parameters, and there is no problem of solvent residue; finally, the extract, longan pulp paste, oligoxylose, and brown algae oligosaccharide are stirred and mixed in a mass ratio of 10: 5-6: 0.5-1: 0.5-0.8 to obtain a lipid-lowering ointment, which has a good lipid-lowering effect. The longan pulp, chrysanthemum notoginseng, and kudzu vine in this product are all food raw materials, and have good edible safety.

[0024] 2. Ultrasonic heating extraction of Glehnia littoralis and Puerariae lobata was performed twice with purified water. The inventors found that by extracting in two stages with different parameters, first extracting under low temperature and low ultrasonic frequency conditions, and then extracting under high temperature and high frequency conditions, the heat-sensitive components in Glehnia littoralis and Puerariae lobata can be retained to the greatest extent, and the protein content in the obtained lipid-lowering ointment reached 26.8%, and the total flavonoids content reached 5.4%.

[0025] 3. After adding xylooligosaccharides and brown algae oligosaccharides to the extract, the taste of the paste is effectively improved, which has a synergistic effect on slowing down the decline of total flavonoids and improves the storage stability of the paste.

[0026] 4. The present invention provides a method for preparing a lipid-lowering ointment, which has no solvent residue, simple process flow, strong operability, low production cost, and can be used for large-scale production of lipid-lowering ointments. The prepared lipid-lowering ointment uses food raw materials as the main raw materials, does not need to add preservatives, has good stability, has a good lipid-lowering effect, can be widely used in medicine and lipid-lowering functional foods, and has great economic value. DETAILED DESCRIPTION

[0027] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiment of the present invention will be clearly and completely described below in combination with the embodiment of the present invention. Obviously, the described embodiment is only an embodiment of a part of the present invention, not all embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work should fall within the scope of protection of the present invention.

[0028] The reagents or instruments involved in the present invention, if the manufacturer is not indicated, are all common commercial products and can be purchased from the market. The experimental methods in the following examples are all conventional methods unless otherwise specified.

[0029] The present invention provides a method for preparing a blood lipid-lowering ointment, comprising the following steps:

[0030] S1. After cleaning and removing impurities from the longan pulp, the longan pulp was crushed through a 60-80 mesh sieve and boiled in a water-tight steamer for 6-8 hours to obtain a longan pulp paste;

[0031] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3-4, and crush them through a 40-60 mesh sieve; add 8-10 times the total weight of purified water, perform the first heating ultrasonic extraction, and collect the first filtrate; add 4-6 times the total weight of purified water to the remaining filter residue, perform the second heating ultrasonic extraction, and collect the second filtrate;

[0032] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.08-1.18 g / cm 3 , obtaining an extract; stirring and mixing the extract, longan pulp paste, xylooligosaccharide and brown algae oligosaccharide in a mass ratio of 10:5-6:0.5-1:0.5-0.8 to obtain a lipid-lowering ointment.

[0033] Longan pulp is rich in nutritional value. After being crushed through a 60-80 mesh sieve, it is boiled in a water-bathed steamer for 6-8 hours. The obtained longan pulp paste has a delicate taste and retains the effective ingredients in the longan pulp. After being cooked, it can improve the storage stability of the later blood lipid-lowering ointment.

[0034] Panax notoginseng and kudzu vine are both medicinal and edible plants. After being crushed, they were extracted twice with purified water. The extract was then concentrated under reduced pressure to a density of 1.08-1.18 g / cm 3 In this density range, the extract has a certain fluidity and viscosity, which is convenient for mixing evenly with oligoxylose and brown algae oligosaccharides. Too high an extract concentration will lead to uneven mixing, while too low a concentration will affect the stability of the finished ointment.

[0035] Oligofructose and brown algae oligosaccharide are water-soluble and stable, which can help enhance human immunity. While giving the paste a slightly sweet taste, they will not increase the body's blood sugar concentration. Oligofructose can selectively promote the proliferation activity of intestinal bifidobacteria. Studies have found that the combination of oligofructose and brown algae oligosaccharide has a synergistic effect on slowing down the decline of total flavonoids and improving the storage stability of the paste.

[0036] Furthermore, the method further comprises the following steps: S4, packaging the blood lipid lowering ointment into glass bottles, with each bottle containing 20-100 g; sealing the glass bottle mouths with heating aluminum foil, and covering the bottle caps after sealing to obtain the blood lipid lowering ointment finished product.

[0037] In order to ensure the storage stability and portability of the ointment, the lipid-lowering ointment is further packaged into 20-100g bottles. The bottle mouth is sealed with aluminum foil, which has good barrier properties and can prevent external microorganisms from entering the ointment and affecting the quality of the ointment. At the same time, the small packaging specifications are adopted, which are convenient to carry and can ensure that the ointment can be consumed in a short period of time. While ensuring product quality, it can also avoid adding additional preservatives to bring burdens to health.

[0038] Furthermore, in S2, the temperature of the first heating ultrasonic extraction is 60-70°C, the ultrasonic frequency is 30-40KHz, and the time is 1-2h.

[0039] Furthermore, in S2, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, and the time is 1.5h.

[0040] Furthermore, in S2, the temperature of the second heating ultrasonic extraction is 80-90°C, the ultrasonic frequency is 50-70KHz, and the time is 1-2h.

[0041] Furthermore, in S2, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, and the time is 1.2h.

[0042] The flat chrysanthemum ginseng and kudzu vine were extracted twice by ultrasonic heating with purified water. The inventor found that by extracting in two stages with different parameters, first extracting under low temperature and low ultrasonic frequency conditions, and then extracting under high temperature and high frequency conditions, the heat-sensitive components in the flat chrysanthemum ginseng and kudzu vine can be retained to the greatest extent. The protein content of the obtained hypolipidemic ointment reached 26.8%, the total flavonoid content reached 5.4%, and there was no solvent residue problem, so it was safe to eat.

[0043] Furthermore, in S2, the mass ratio of the extract, longan pulp paste, xylooligosaccharide, and brown algae oligosaccharide is 10:5.5:0.8:0.6. The inventors found that when the mass ratio of the extract, longan pulp paste, xylooligosaccharide, and brown algae oligosaccharide is 10:5.5:0.8:0.6, the synergistic effect of lowering blood lipids is better.

[0044] The second object of the present invention is to provide a blood lipid-lowering ointment prepared by the above preparation method.

[0045] The third object of the present invention is to provide the application of the above-mentioned blood lipid-lowering ointment in medicine and blood lipid-lowering functional food. The blood lipid-lowering ointment prepared by the present invention uses food raw materials as main raw materials, does not need to add preservatives, has a good blood lipid-lowering effect, and is safer to eat.

[0046] The present invention is further described in detail below in conjunction with specific embodiments:

[0047] Example 1: Preparation of blood lipid-lowering ointment

[0048] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0049] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, and perform the first heating ultrasonic extraction; the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, and perform the second heating ultrasonic extraction; the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the second filtrate is collected;

[0050] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:5.5:0.8:0.6 to obtain a lipid-lowering ointment.

[0051] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0052] Example 2: Preparation of blood lipid-lowering ointment

[0053] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through an 80-mesh sieve, and boiled in a water-tight steamer for 6 hours to obtain a longan pulp paste;

[0054] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3, and crush them through a 60-mesh sieve; add 8 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 60°C, the ultrasonic frequency is 30KHz, the time is 1h, and the first filtrate is collected. Add 4 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 80°C, the ultrasonic frequency is 50KHz, the time is 1h, and the second filtrate is collected;

[0055] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.18 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:5:0.5:0.5 to obtain a lipid-lowering ointment.

[0056] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0057] Example 3: Preparation of blood lipid-lowering ointment

[0058] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 8 hours to obtain a longan pulp paste;

[0059] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:4, and crush them through a 60-mesh sieve; add 8 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 70°C, the ultrasonic frequency is 40KHz, the time is 2h, and the first filtrate is collected. Add 6 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 90°C, the ultrasonic frequency is 70KHz, the time is 2h, and the second filtrate is collected;

[0060] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.08 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:6:1:0.8 to obtain a lipid-lowering ointment.

[0061] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0062] Comparative Example 1: Preparation of blood lipid-lowering ointment

[0063] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0064] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the second filtrate is collected;

[0065] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:5.5:0.8:0.6 to obtain a lipid-lowering ointment.

[0066] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0067] Comparative Example 2: Preparation of blood lipid-lowering ointment

[0068] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0069] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the second filtrate is collected;

[0070] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:5.5:0.8:0.6 to obtain a lipid-lowering ointment.

[0071] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0072] Comparative Example 3: Preparation of blood lipid-lowering ointment

[0073] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0074] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the second filtrate is collected;

[0075] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide were mixed in a mass ratio of 10:5.5:0.8:0.6 to obtain a lipid-lowering ointment.

[0076] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0077] Comparative Example 4: Preparation of blood lipid-lowering ointment

[0078] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0079] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the second filtrate is collected;

[0080] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste and xylooligosaccharide were mixed in a mass ratio of 10:5.5:0.8 to obtain a lipid-lowering ointment.

[0081] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0082] Comparative Example 5: Preparation of blood lipid-lowering ointment

[0083] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0084] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the second filtrate is collected;

[0085] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3 The extract, longan pulp paste and brown algae oligosaccharide were mixed in a mass ratio of 10:5.5:0.6 to obtain a lipid-lowering ointment.

[0086] S4, the blood lipid-lowering ointment is packaged in glass bottles, each bottle having a volume of 100 g. The mouth of the glass bottle is sealed by heating with an aluminum foil sheet, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0087] Comparative Example 6: Preparation of blood lipid-lowering ointment

[0088] S1. After cleaning and removing impurities, 500 g of longan pulp was crushed through a 60-mesh sieve, and boiled in a water-tight steamer for 7 hours to obtain a longan pulp paste;

[0089] S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3.5, and crush them through a 40-mesh sieve; add 9 times the total weight of purified water, perform the first heating ultrasonic extraction, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, the time is 1.5h, and the first filtrate is collected. Add 5 times the purified water to the remaining filter residue, perform the second heating ultrasonic extraction, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, the time is 1.2h, and the second filtrate is collected;

[0090] S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.12 g / cm 3The extract and the longan pulp paste were stirred and mixed in a mass ratio of 10:5.5 to obtain a lipid-lowering ointment.

[0091] S4. The blood lipid-lowering ointment is packaged in glass bottles, with each bottle containing 100g. The mouth of the glass bottle is sealed with aluminum foil, and the bottle cap is put on after sealing to obtain the finished blood lipid-lowering ointment.

[0092] Test Example 1: Physical and Chemical Property Testing

[0093] The physicochemical properties of the hypolipidemic ointment extracted from Examples 1-3 and Comparative Examples 1-6 were evaluated, and the sensory, density, total flavonoids, and protein content of the hypolipidemic ointment were investigated by conventional methods. The density test method was determined by referring to the relative density determination method (General Rule 0601) of the Chinese Pharmacopoeia, the total flavonoids test method was determined by referring to the "Technical Guidelines for Inspection and Evaluation of Physical, Chemical and Hygiene Indicators of Health Foods (2020 Edition)" "Determination of Total Flavonoids in Health Foods", and the protein test method was determined by referring to GB5009.5-2016 "Determination of Protein in Foods of National Food Safety Standards", and the results are shown in Table 1.

[0094] Table 1 Comparison of the physicochemical properties of hypolipidemic ointments

[0095]

[0096] As can be seen from Table 1, the density of the lipid-lowering ointment prepared in Examples 1-3 ranges from 1.39 to 1.42 g / cm 3 At this density, the lipid-lowering ointment is in a semi-fluid state and is not easy to harden during the later storage process. The study also found that when the density is too high, the lipid-lowering ointment is easy to clump and difficult to disperse during the later use. The density of the lipid-lowering ointment prepared in Comparative Examples 4-6 is 1.25-1.33 g / cm 3 The density is lower than that of Example 1-3. It can be seen that the addition of xylo-oligosaccharide and brown algae oligosaccharide is beneficial to controlling the density of the blood lipid-lowering cream.

[0097] As can be seen from Table 1, the hypolipidemic ointment prepared in Example 1 has the highest total flavonoids and protein content and the best extraction effect. The total flavonoids content of the hypolipidemic ointment prepared in Example 1 is 5.4%, which is much higher than that of Comparative Examples 1-3, and the protein content is 26.8%, which is slightly higher than that of Comparative Examples 1-3. It can be seen that through two phased extractions with different parameters, first under low temperature and low ultrasonic frequency conditions, and then under high temperature and high frequency conditions, the heat-sensitive components in Glehnia littoralis and Puerariae can be retained to the greatest extent, and the total flavonoids and protein content obtained by extraction are relatively high. In Comparative Example 1, the extraction was first carried out under high temperature and high frequency conditions, and then under low temperature and low ultrasonic frequency conditions. The total flavonoids and protein contents of the prepared lipid-lowering ointment were 4.5% and 23.6%, respectively; in Comparative Examples 2 and 3, the same technical parameters were used for the two extractions, and the total flavonoids contents were 3.4% and 3.8%, respectively, and the protein contents were 22.9% and 23.2%, respectively. It can be seen that the same technical parameters were used for two water extractions, and the extraction efficiency of total flavonoids and protein was low.

[0098] Test Example 2: Stability Test

[0099] The lipid-lowering ointments prepared in Examples 1-3 and Comparative Examples 4-6 were subjected to a 3-month stability test. The samples were placed at a temperature of 37±2°C and a relative humidity of 75±5% for stability testing. The density, total flavonoids and protein content were measured, and the results are shown in Table 2.

[0100] Table 2 Stability test results

[0101]

[0102]

[0103] As can be seen from Table 2, the density of the lipid-lowering ointments prepared in Examples 1-3 and Comparative Examples 4-6 increases with time, which may be related to the evaporation of water during storage. The total flavonoids and protein content of the lipid-lowering ointments prepared in Examples 1-3 did not change much after three months.

[0104] The lipid-lowering ointment prepared in Comparative Example 4 had a total flavonoid content of 5.3% and a protein content of 26.7% at 0 month, and a total flavonoid content of 4.1% and a protein content of 24.9% after 3 months. The total flavonoid content decreased by 22.64% and the protein content decreased by 6.74%.

[0105] The lipid-lowering ointment prepared in Comparative Example 5 had a total flavonoid content of 5.1% and a protein content of 26.6% at 0 month, and a total flavonoid content of 4.0% and a protein content of 25.1% after 3 months. It can be seen that after three months, the total flavonoid content of the lipid-lowering ointment prepared in Comparative Example 5 decreased by 21.57% and the protein content decreased by 5.64%.

[0106] The lipid-lowering ointment prepared in Comparative Example 6 had a total flavonoid content of 5.2% and a protein content of 26.4% at 0 month, and a total flavonoid content of 3.5% and a protein content of 24.5% after 3 months. It can be seen that after three months, the total flavonoid content of the lipid-lowering ointment prepared in Comparative Example 6 decreased by 32.69% and the total flavonoid content decreased by 7.20%.

[0107] It can be seen from the results of Example 1 and Comparative Examples 4-6 that the addition of xylooligosaccharides and brown algae oligosaccharides to the lipid-lowering ointment has a synergistic effect on slowing down the decline of total flavonoids and improving the storage stability of the ointment.

[0108] Test Example 3: Investigation of blood lipid lowering effect

[0109] 60 volunteers aged 35-65 were recruited and randomly divided into 3 groups. They took the lipid-lowering ointment prepared in Examples 1-3, once in the morning and once in the evening, 20 g each time, for 14 consecutive days. Before taking the lipid-lowering ointment on the first day of enrollment and 2 hours after taking the lipid-lowering ointment for the last time on the 14th day, the total cholesterol, triglyceride and high-density cholesterol levels of the subjects were tested using a lipidometer, and the average changes were calculated. The results are shown in Table 3. "-" in the table indicates a decrease in content.

[0110] Table 3

[0111] Total cholesterol change Change in triglycerides Changes in blood high-density cholesterol Example 1 -3.5％ -4.2％ -3.8％ Example 2 -3.2％ -3.9％ -3.5％ Example 3 -3.1％ -4.0％ -3.4％

[0112] As can be seen from Table 3, after taking the lipid-lowering ointments prepared in Examples 1-3 for 14 days, the total cholesterol, triglyceride and high-density cholesterol levels of the test population were reduced to varying degrees, indicating that the lipid-lowering ointment prepared in the present invention has a good lipid-lowering effect.

[0113] The present invention uses longan pulp, chrysanthemum notoginseng and kudzu vine as main raw materials when preparing the blood lipid-lowering ointment, wherein the longan pulp is directly boiled in a water-steaming pot for 6-8 hours after being crushed, effectively retaining the effective ingredients in the longan pulp, and sterilizing the longan raw materials, which is beneficial to improving the storage stability of the blood lipid-lowering ointment and is not easy to deteriorate; the chrysanthemum notoginseng and kudzu vine are subjected to ultrasonic heating extraction twice with purified water, and the heat-sensitive components in the chrysanthemum notoginseng and kudzu vine can be retained to the greatest extent through two phased extractions with different parameters, and there is no problem of solvent residue; finally, the extract, longan pulp paste, oligoxylose and brown algae oligosaccharide are stirred and mixed in a mass ratio of 10:5-6:0.5-1:0.5-0.8 to obtain the blood lipid-lowering ointment. The blood lipid-lowering ointment has a good blood lipid-lowering effect, and the longan pulp, chrysanthemum notoginseng and kudzu vine are all food raw materials, have good edible safety, simple preparation process, are suitable for industrial large-scale production, and have good economic benefits.

[0114] The above contents are only for explaining the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the claims of the present invention.

Claims

1. A method for preparing a blood lipid-lowering ointment, characterized in that: The steps include: S1. After cleaning and removing impurities from the longan pulp, the longan pulp was crushed through a 60-80 mesh sieve and boiled in a water-tight steamer for 6-8 hours to obtain a longan pulp paste; S2, after removing impurities from the flat chrysanthemum Panax notoginseng and kudzu vine, cut them into sections, mix them in a mass ratio of 1:3-4, and crush them through a 40-60 mesh sieve; add 8-10 times the total weight of purified water, perform the first heating ultrasonic extraction, and collect the first filtrate; add 4-6 times the total weight of purified water to the remaining filter residue, perform the second heating ultrasonic extraction, and collect the second filtrate; S3, the two filtrates obtained in S2 were combined and concentrated under reduced pressure to a density of 1.08-1.18 g / cm 3 , obtaining an extract; stirring and mixing the extract, longan pulp paste, xylooligosaccharide and brown algae oligosaccharide in a mass ratio of 10:5-6:0.5-1:0.5-0.8 to obtain a lipid-lowering ointment.

2. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: Also includes the steps: S4. The lipid-lowering ointment is packaged in glass bottles, with each bottle containing 20-100 g; the mouth of the glass bottle is sealed with a heat-sealed aluminum foil, and the bottle cap is put on after sealing to obtain the finished lipid-lowering ointment.

3. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: In S2, the temperature of the first heating ultrasonic extraction is 60-70°C, the ultrasonic frequency is 30-40KHz, and the time is 1-2h.

4. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: In S2, the temperature of the first heating ultrasonic extraction is 65°C, the ultrasonic frequency is 35KHz, and the time is 1.5h.

5. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: In S2, the temperature of the second heating ultrasonic extraction is 80-90°C, the ultrasonic frequency is 50-70KHz, and the time is 1-2h.

6. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: In S2, the temperature of the second heating ultrasonic extraction is 85°C, the ultrasonic frequency is 60KHz, and the time is 1.2h.

7. The method for preparing a blood lipid-lowering ointment according to claim 1, characterized in that: In S2, the mass ratio of the extract, longan pulp paste, xylo-oligosaccharide and brown algae oligosaccharide is 10:5.5:0.8:0.

6.

8. A blood lipid lowering ointment prepared by the preparation method according to any one of claims 1 to 7.

9. Use of the blood lipid lowering ointment as claimed in claim 8 in medicine and blood lipid lowering functional food.

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