Awaking mouse head fixing instrument capable of being adjusted in multiple dimensions

Abstract

The invention provides a sober mouse head fixing instrument capable of being adjusted in multiple dimensions, which comprises a first adjusting assembly, a second adjusting assembly and a window patch, and is characterized in that the first adjusting assembly is arranged on a chassis and has a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis; the second adjusting assembly is arranged on the first adjusting assembly and has the rotation freedom degree in the first direction and the rotation freedom degree in the third direction relative to the chassis. And the window patch is arranged on the second adjusting assembly and has a displacement freedom degree in a plane formed in the second direction and the third direction relative to the chassis. Through the arrangement of the first adjusting assembly, the second adjusting assembly and the window patch, the fixing instrument for fixing the part, needing to be imaged, of an animal and performing multi-dimensional adjustment to be matched with imaging is achieved, the movement of the animal cannot be completely restrained, and the purposes that the head of a sober mouse is fixed, and behavioral experiment requirements can be met are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of animal experiment instruments, in particular to a multi-dimensionally adjustable head fixer for awake mice. Background Art

[0002] The brain plays an important role in life activities and is the most complex organ in living organisms. The study of brain function plays a very important role in neurobiology. At present, animal models are commonly used as research objects in neurobiological research, such as mice and macaques. If you want to study the brain function of experimental animals, you usually perform microscopic imaging of blood vessels or neurons in the animal's brain.

[0003] In terms of microscopic imaging, two-photon microscopy has been widely used in functional imaging of living animals due to its advantages of deep penetration, low scattering and deep imaging. In functional imaging of living animals, awake mice can respond in combination with behavior, have real physiological reactions, and are more convenient for conducting physiological studies on related neurons. Therefore, it is very important to have a device that can be used to fix the head of awake mice and match behavior.

[0004] In the technology of fixing the head of awake mice, there are instruments that can accurately adjust the angles of the two axes, AP (anteroposterior bregma) and ML (anteroposterior raphe), and each axis has a range greater than 15 degrees. However, in actual use, the body of the mouse is completely in the restraint tube and cannot move autonomously, which is not suitable for behavioral experiments. In addition, the instrument design process is complex and expensive.

[0005] There is also a head fixator for awake mice used in experiments, which adds a head adjustment device to the restraint tube, but the mouse's body is still completely in the restraint tube, and in actual use, the head adjustment device will conflict with the objective lens space during imaging.

[0006] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present invention and does not constitute any limitation to the present invention. Summary of the invention

[0007] In view of the shortcomings of the prior art mentioned above, the present invention provides a multi-dimensionally adjustable head fixer for awake mice. By setting a first adjustment component, a second adjustment component and a window patch, the fixation of the animal's imaging part and the multi-dimensionally adjustable fixer for coordinated imaging are achieved, and the animal's own activities will not be completely restrained, so as to solve the problem of fixing the head of awake mice and matching the needs of behavioral experiments.

[0008] The present invention provides a multi-dimensionally adjustable head fixation device for awake mice, comprising:

[0009] A first adjustment component, the first adjustment component is mounted on the chassis and has a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis;

[0010] a second adjustment component, the second adjustment component being mounted on the first adjustment component and having a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis; and

[0011] The window patch is mounted on the second adjustment component and has the freedom of displacement within the plane formed by the second direction and the third direction relative to the chassis.

[0012] In one embodiment of the present invention, a surface of the chassis on which the first adjustment component is mounted is provided with a step, the step is provided along the contour of the chassis, and a gap is left between the step and the edge of the chassis surface to form an outer contour plane at the edge of the chassis.

[0013] In one embodiment of the present invention, the first adjustment component includes a height adjustment frame and a crossbeam. The bottom end of the height adjustment frame is installed on the chassis, and a through slot is opened on its upper part along the first direction; the end of the crossbeam is installed on the through slot through a connecting piece, and the crossbeam can rotate along the second direction.

[0014] In one embodiment of the present invention, the height adjustment frame is further provided with angle scale lines, and the angle scale lines are used to measure the rotation angle of the crossbeam in the through slot in the second direction.

[0015] In one embodiment of the present invention, a through wedge-shaped groove is formed on the crossbeam along the first direction, and a through screw hole is formed in the wedge-shaped groove along the third direction.

[0016] In one embodiment of the present invention, the second component includes an adapter plate and a fixed plate. The adapter plate is installed on the beam through a connecting member, and the adapter plate can rotate along a third direction; the fixed plate is installed at the end of the adapter plate through a connecting member, and the fixed plate can rotate along the first direction.

[0017] In one embodiment of the present invention, the second adjustment component includes an adjustment neck, an adapter plate and a fixed plate, the adjustment neck is installed in the wedge-shaped groove; the adapter plate is installed on the adjustment neck through a connecting member, and the adapter plate can rotate along a third direction; the fixed plate is installed on the end of the adapter plate through a connecting member, and the fixed plate can rotate along the first direction.

[0018] In one embodiment of the present invention, a through hole is provided on the adjusting neck, and a connecting hole corresponding to the through hole is provided on the adapter plate. The adjusting neck and the adapter plate are connected by bolts passing through the through hole and the connecting hole, so that the adapter plate can rotate along the third direction; the adjusting neck is locked and positioned on the crossbeam by a screw passing through the screw hole and abutting against the adjusting neck in the wedge-shaped groove, so that the adjusting neck can move along the first direction.

[0019] In one embodiment of the present invention, a U-shaped groove penetrating the first direction is formed on the fixing plate, and a plurality of pairs of positioning holes penetrating the first direction are formed on the fixing plate on both sides of the U-shaped groove.

[0020] In one embodiment of the present invention, the window patch includes a central hole and positioning grooves located on both sides of the central hole. The central hole is used to expose the imaging observation part, and the window patch is installed on a plurality of pairs of positioning holes through the positioning grooves.

[0021] The beneficial effects of the present invention are as follows: by setting up the first adjustment component, the second adjustment component and the window patch, the spatial displacement degrees of freedom and the rotation degrees of freedom that exist respectively are combined to achieve fixation of the animal's imaging part and multi-dimensional adjustment coordinated imaging fixation instrument, and the animal's own activities will not be completely restrained, which is conducive to fully observing the activities of the animal object during the experiment, and is suitable for behavioral experiments and two-photon imaging coordinated experiments.

[0022] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings herein are incorporated into the specification and constitute a part of the specification, showing embodiments consistent with the present invention, and together with the specification, are used to explain the principles of the present invention. Obviously, the drawings described below are only some embodiments of the present invention, and for those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0024] Figure 1 It is a schematic structural diagram of the fixing device capable of multi-dimensional adjustment in the first viewing angle of the present invention;

[0025] Figure 2 It is a schematic diagram of the structure of the multi-dimensionally adjustable fixing device of the present invention at a second viewing angle;

[0026] Figure 3 It is a structural schematic diagram of the chassis in the present invention;

[0027] Figure 4 It is a structural schematic diagram of the height adjustment frame in the present invention;

[0028] Figure 5 It is a structural schematic diagram of the crossbeam in the present invention;

[0029] Figure 6 It is an exploded schematic diagram of the second adjustment component structure in the present invention;

[0030] Figure 7 It is an exploded schematic diagram of the fixing plate and the window patch structure of the present invention;

[0031] Figure 8 It is a structural schematic diagram of the window patch in the present invention.

[0032] In the figure: 10, chassis; 101, screw hole; 102, step; 103, outer contour plane; 1, first adjustment component; 11, height adjustment frame; 110, round hole; 111, through slot; 112, angle scale line; 12, crossbeam; 120, threaded hole; 121, wedge-shaped slot; 122, screw hole; 2, second adjustment component; 21, adjustment neck; 210, through hole; 22, adapter plate; 220, connecting hole; 23, fixing plate; 230, through hole; 231, U-shaped slot; 232, positioning hole; 3, window patch; 31, center hole; 32, positioning slot. DETAILED DESCRIPTION

[0033] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied by other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the features in the following embodiments and embodiments can be combined with each other without conflict. It should also be understood that the terms used in the embodiments of the present invention are to describe specific specific embodiments, rather than to limit the scope of protection of the present invention.

[0034] See also Figures 1 to 8 . It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any structural modification, change in proportional relationship, or adjustment of size, without affecting the effects and purposes that can be achieved by the present invention, should still fall within the scope of the technical content disclosed by the present invention. At the same time, the terms such as position and quantitative relationship cited in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0035] See also Figure 1 and Figure 2 The present invention provides a multi-dimensionally adjustable head fixation device for awake mice, comprising:

[0036] A first adjustment component 1, the first adjustment component 1 is mounted on the chassis 10 and has a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis 10;

[0037] A second adjustment component 2, which is mounted on the first adjustment component 1 and has a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis 10; and

[0038] The window patch 3 is mounted on the second adjustment component 2 and has the freedom of displacement relative to the chassis 10 within the plane formed by the second direction and the third direction.

[0039] Specifically, in the embodiment of the present invention, the chassis 10 serves as the base of the fixing device, can provide stable support, and can be fixed on the laboratory table. The first adjustment component 1 is installed on the chassis 10 and provides corresponding spatial freedom. On this basis, the second adjustment component 2 is installed on the first adjustment component 1, which also provides new spatial freedom. Finally, the window patch 3 installed on the second adjustment component 2 can also provide corresponding spatial freedom. In this way, the spatial freedom formed by the three structures is matched, the animal body parts to be imaged in the experiment are fixed and a multi-dimensional adjustment function is provided.

[0040] More specifically, the first adjustment component 1 provides a displacement degree of freedom in a first direction relative to the chassis 10 and a rotational degree of freedom in a second direction, for example, a slide rail device can be used to perform translational adjustment along the vertical axis (i.e., the first direction) of the chassis 10, or the chassis 10 can be connected by a rotation axis to allow the first adjustment component 1 to rotate around a horizontal axis (i.e., the second direction). The second adjustment component 2 provides a rotational degree of freedom in a first direction relative to the chassis 10 and a rotational degree of freedom in a third direction, for example, it can be made to rotate around a vertical axis (the first direction) relative to the chassis 10, and the component can rotate around an axis perpendicular to the first direction (the third direction) at the same time. The window patch 3 can be made of titanium alloy and bonded to an animal experimental object such as a mouse skull using dental cement. It has a light weight and good hardness and is suitable for long-term experiments. In the structure of the window patch 3, a slide rail or a plurality of positioning points can be used to achieve displacement movement, so as to move in the plane formed by the second direction and the third direction (relative to the horizontal plane of the chassis 10).

[0041] It should be noted that, through the different components in the first adjustment component 1, the second adjustment component 2 and the window patch 3, for example, sliding connection methods such as slide rails are used, and rotating connection methods such as screw locking mechanisms are equipped to maintain the desired angle, so that relative displacement or rotational freedom is formed between the components, which will not be elaborated here.

[0042] In this way, by setting up the first adjustment component 1, the second adjustment component 2 and the window patch 3, through the combination of the existing spatial displacement degrees of freedom and rotational degrees of freedom, the head of the experimental object such as a mouse can be stably fixed, and the fixation of the animal's imaging part and the multi-dimensional adjustment and coordinated imaging fixation device can be achieved, and the animal's own activities will not be completely restrained, which is conducive to fully observing the activities of the animal object during the experiment. It is suitable for the coordination of behavioral experiments and two-photon imaging.

[0043] See also Figure 1 and Figure 3 In one embodiment, a step 102 is provided on the surface of the chassis 10 on which the first adjustment component 1 is installed. The step 102 is provided along the contour of the chassis 10 , and a gap is left between the step 102 and the edge of the surface of the chassis 10 to form an outer contour plane 103 at the edge of the chassis 10 .

[0044] Specifically, in the embodiment of the present invention, the chassis 10 can be a circular plane in the shape of a disk, and corresponding screw holes 101 are provided on the chassis 10 for connecting the fixedly installed first adjustment component 1. The step 102 provided on the surface of the chassis 10 is used to form an accommodating space on the surface of the chassis 10 to ensure that the excrement of the experimental object such as a mouse during the fixation period will not contaminate the external space of the chassis 10 such as the laboratory table, and the outer contour plane 103 formed by the step 102 can be used as a positioning surface of the fixing device to fix the fixing device as a whole on the laboratory table.

[0045] See also Figure 1 and Figure 2 , Figure 4 and Figure 5 In one embodiment, the first adjustment component 1 includes a height adjustment frame 11 and a crossbeam 12. The bottom end of the height adjustment frame 11 is mounted on the chassis 10, and a through slot 111 is opened on its upper portion along the first direction; the end of the crossbeam 12 is mounted on the through slot 111 through a connecting piece, and the crossbeam 12 can rotate along the second direction.

[0046] Specifically, in the embodiment of the present invention, the first adjustment component 1 is formed by the structure of the height adjustment frame 11 and the crossbeam 12. The height adjustment frame 11 can be set to be L-shaped, and a round hole 110 corresponding to the screw hole 101 of the chassis 10 is opened at its bottom for screw connection and fixing. The through slot 111 opened at the upper part of the height adjustment frame 11 is used to install the end of the crossbeam 12, and the crossbeam 12 can select a specific fixed position in the through slot 111, that is, the crossbeam 12 can move up and down along the through slot 111 to adjust the height, thereby forming a displacement degree of freedom in the first direction. Similarly, a threaded hole 120 is opened at the end of the crossbeam 12 connected to the through slot 111, and the aperture of the threaded hole 120 matches the width of the through slot 111, so that a hand screw can be used to install and connect to the threaded hole 120 and lock and fix it on the through slot 111. That is to say, the connecting member can be represented by the threaded hole 120 opened on the crossbeam 12 and the hand screw used. In this way, the position of the beam 12 in the through slot 111 can be adjusted by tightening the thumb screw, and the angle of the beam 12 can be adjusted during the process of tightening the thumb screw, that is, a rotational degree of freedom in the second direction is formed, so that the axial rotation of the thumb screw can be used to adjust the angle of the experimental object such as the AP (front end to rear end) of the mouse.

[0047] See also Figure 4 , the height adjustment frame 11 is also provided with angle scale lines 112, which are used to measure the rotation angle of the cross beam 12 in the through slot 111 in the second direction. By setting scale lines at corresponding positions of the height adjustment frame 11, for example, by posting or engraving angle scale lines 112 on one side of both ends of the through slot 111, the corresponding angle of the cross beam 12 adjustment is indicated for intuitive operation.

[0048] See also Figure 5 A through wedge-shaped groove 121 is provided on the cross beam 12 along the first direction, and a screw hole 122 is provided in the wedge-shaped groove 121 to pass through the third direction. The wedge-shaped groove 121 and the screw hole 122 provided therein can be abutted against a corresponding component of the second adjustment component 2, such as the adjustment neck 21, by a hand screw installed in the screw hole 122, so as to provide the second adjustment component 2 with a degree of freedom of movement relative to the cross beam 12 in the first direction.

[0049] In one embodiment, the second component includes an adapter plate 22 and a fixed plate 23. The adapter plate 22 is installed on the beam 12 through a connecting member, and the adapter plate 22 can rotate along the third direction; the fixed plate 23 is installed at the end of the adapter plate 22 through a connecting member, and the fixed plate 23 can rotate along the first direction.

[0050] Specifically, in the embodiment of the present invention, the second adjustment component 2 (not shown in the drawings) can be directly formed by the adapter plate 22 and the fixed plate 23. That is to say, it is not necessary to provide the wedge-shaped groove 121 on the crossbeam 12 and the corresponding adjustment neck 21 structure, and the second adjustment component 2 can also provide rotational freedom in two directions. That is, by providing corresponding through holes 210 and connecting holes 220 in the third direction and the first direction corresponding to the adapter plate 22 and the fixed plate 23, and connecting them with corresponding hand screws (that is, the connecting parts used can be realized by locking bolts or hand screws according to the fixing or activity requirements of the relevant components, which will not be repeated in the embodiment of the present invention), the rotation adjustment in the corresponding directions can be realized respectively.

[0051] See also Figure 6 In one embodiment, the second adjustment component 2 includes an adjustment neck 21, an adapter plate 22 and a fixed plate 23. The adjustment neck 21 is installed in the wedge-shaped groove 121; the adapter plate 22 is installed on the adjustment neck 21 through a connecting piece, and the adapter plate 22 can rotate along the third direction; the fixed plate 23 is installed on the end of the adapter plate 22 through a connecting piece, and the fixed plate 23 can rotate along the first direction.

[0052] Furthermore, a through hole 210 is provided on the adjusting neck 21, and a connecting hole 220 corresponding to the through hole 210 is provided on the adapter plate 22. The adjusting neck 21 and the adapter plate 22 are connected by bolts passing through the through hole 210 and the connecting hole 220, so that the adapter plate 22 can rotate along the third direction; the adjusting neck 21 is locked and positioned on the beam 12 by a screw passing through the screw hole 122 and abutting against the adjusting neck 21 in the wedge-shaped groove 121, so that the adjusting neck 21 can move along the first direction.

[0053] Specifically, in the embodiment of the present invention, the second adjustment component 2 is composed of an adjustment neck 21, an adapter plate 22 and a fixing plate 23. The adjustment neck 21 is matched and fixed with the wedge-shaped groove 121 in the crossbeam 12. The through hole 210 provided in the adjustment neck 21 is used to match and fix with the connection hole 220 and the nut groove in the adapter plate 22. For example, a screw can be passed through the corresponding through hole 210, the connection hole 220 and the nut groove, and a nut can be installed in the nut groove to complete the connection. The adapter plate 22 can be rotated along the fixed axis of the screw (i.e., the third direction) to adjust the angle of the mouse ML (inside to outside), and can be locked by the corresponding screw and nut structure after adjustment.

[0054] Similarly, through the wedge-shaped groove 121 and the screw hole 122 opened therein, the hand screw installed in the screw hole 122 can be abutted against the adjustment neck 21 in the wedge-shaped groove 121 to meet the displacement requirement of the second adjustment component 2 in the first direction. At the same time, in order to meet the rotation requirement of the fixing plate 23 in the first direction, corresponding through holes 230 along the first direction can be opened on the adapter plate 22 and the fixing plate 23, and corresponding connecting parts such as screws and nuts can also be used to adjust the orientation of the entire body of the mouse, and after adjustment, it can be locked by the screw and nut structure.

[0055] See also Figure 7 In one embodiment, a U-shaped groove 231 penetrating the first direction is formed on the fixing plate 23 , and a plurality of pairs of positioning holes 232 penetrating the first direction are formed on the fixing plate 23 on both sides of the U-shaped groove 231 .

[0056] Specifically, in the embodiment of the present invention, the fixing plate 23 is used to install the window patch 3, which is directly installed on the animal body part such as the head of the mouse, and the U-shaped groove 231 is provided to leave a space for the animal body part such as the mouse body to move. On the fixing plate 23 formed on both sides of the U-shaped groove 231, a plurality of pairs of positioning holes 232 are provided to selectively fix the experimental animal object such as the mouse with the window patch 3 attached to the position of a pair of positioning holes 232 as needed.

[0057] See also Figure 8 In one embodiment, the window patch 3 includes a center hole 31 and positioning grooves 32 located on both sides of the center hole 31. The center hole 31 is used to expose the imaging observation part, and the window patch 3 is installed on a plurality of pairs of positioning holes 232 through the positioning grooves 32.

[0058] Specifically, the animal experimental object part, such as the mouse cranial window, is exposed through the central hole 31 of the window patch 3 so as not to affect the subsequent imaging observation experiment. The positioning grooves 32 opened on both sides can fix the window patch 3 on the positioning holes 232 of the fixing plate 23 through a bolt structure. At the same time, according to the left and right positions of the brain area of ​​the animal body part, such as the mouse head, the positioning grooves 32 on both sides corresponding to the left and right positions of the brain area can be selected to have different lengths.

[0059] It should be noted that, by virtue of the rotational freedom of the fixing plate 23 in the first direction, the multiple pairs of positioning holes 232 thereon realize the displacement freedom of the window patch 3 to be installed and fixed in one direction, and the size difference between the body shape of the animal experimental subject and the width of the U-shaped groove 231, the displacement freedom of the window patch 3 in another direction is formed, that is, the displacement freedom of the window patch 3 in the plane formed by the second direction and the third direction (the horizontal plane relative to the chassis 10) is realized.

[0060] In one embodiment, the use process of the fixing device may include the following steps:

[0061] Before use, the experimental mouse needs to undergo craniotomy under anesthesia to expose the tissue to be imaged. For some deep brain regions, a prism or a Grin Lens is embedded. After embedding, a glass patch is attached to the tissue, and the skull is sealed and fixed with tissue glue. Then dental cement is applied around the cranial window glass, and the dental cement bonds the window patch 3 to the mouse skull, with the glass patch located at the center hole 31.

[0062] The mouse that recovers normally after operation is fixed on the fixing device in the embodiment of the present invention, the window patch 3 of the mouse head is fixed on the fixing plate 23 with two screws, select suitable fixed distance according to experimental needs, mainly according to the space required for the object lens during animal size and imaging. Move up and down along the through groove 111 and adjust the neck 21 to move up and down along the wedge groove 121 to adjust the height of the mouse, so that the limbs of the mouse contact chassis 10 and can move freely. When carrying out living body two-photon imaging, ensure that the mouse cranial window is vertical to the object lens light, then need to adjust the front and back left and right angles of the mouse in some marginal brain areas, the crossbeam 12 can be used to adjust the angle of the mouse AP (front end to rear end) along the fixed axis rotation, and the adapter plate 22 can be used to adjust the angle of the mouse ML (inboard to outside) along the fixed axis rotation. In addition, the fixing plate 23 can be used to adjust the whole body of the mouse towards along the fixed axis rotation, so that the device direction of the behavior can be more easily matched, such as screen equipment, to complete the experimental process.

[0063] In summary, the present invention provides a multi-dimensionally adjustable head fixer for awake mice. By setting a first adjustment component, a second adjustment component and a window patch, the head of an experimental object such as a mouse can be stably fixed by utilizing the combination of the existing spatial displacement degrees of freedom and rotation degrees of freedom, thereby achieving fixation of the animal's imaging site and multi-dimensionally adjustable coordinated imaging fixator, and the fixation will not completely restrain the animal's own activities, which is conducive to fully observing the activities of the animal object during the experiment, and is suitable for coordinated experiments of behavioral experiments and two-photon imaging.

[0064] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A multi-dimensionally adjustable head fixation device for awake mice, characterized in that: include: A first adjustment component (1), the first adjustment component (1) being mounted on the chassis (10) and having a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis (10); a second adjustment component (2), the second adjustment component (2) being mounted on the first adjustment component (1) and having a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis (10); as well as A window patch (3), the window patch (3) being mounted on the second adjustment component (2) and having a degree of freedom of displacement within a plane formed by a second direction and a third direction relative to the chassis (10).

2. The fixing device according to claim 1, characterized in that: A step (102) is provided on the surface of the chassis (10) on which the first adjustment component (1) is mounted. The step (102) is arranged along the contour of the chassis (10), and a gap is left between the step (102) and the edge of the surface of the chassis (10) so as to form an outer contour plane (103) at the edge of the chassis (10).

3. The fixing device according to claim 1, characterized in that: The first adjustment component (1) comprises: A height adjustment frame (11), the bottom end of the height adjustment frame (11) is mounted on the bottom plate (10), and a through slot (111) is formed on the upper portion thereof along a first direction; and A crossbeam (12), the end of which is mounted on the through slot (111) via a connecting piece, and enables the crossbeam (12) to rotate along a second direction.

4. The fixing device according to claim 3, characterized in that: The height adjustment frame (11) is also provided with an angle scale line (112), and the angle scale line (112) is used to measure the rotation angle of the crossbeam (12) in the through slot (111) in the second direction.

5. The fixing device according to claim 3, characterized in that: A through wedge-shaped groove (121) is provided on the crossbeam (12) along a first direction, and a screw hole (122) is provided in the wedge-shaped groove (121) and passes through in a third direction.

6. The fixing device according to claim 3, characterized in that: The second component comprises: an adapter plate (22), the adapter plate (22) being mounted on the crossbeam (12) via a connecting piece, and enabling the adapter plate (22) to rotate along a third direction; and A fixed plate (23), wherein the fixed plate (23) is mounted on the end of the adapter plate (22) via a connecting piece, and the fixed plate (23) is capable of rotating along a first direction.

7. The fixing device according to claim 5, characterized in that: The second adjustment component (2) comprises: An adjusting neck (21), the adjusting neck (21) being installed in the wedge-shaped groove (121); an adapter plate (22), the adapter plate (22) being mounted on the adjustment neck (21) via a connecting piece, and enabling the adapter plate (22) to rotate along a third direction; and A fixed plate (23), wherein the fixed plate (23) is mounted on the end of the adapter plate (22) via a connecting piece, and the fixed plate (23) is capable of rotating along a first direction.

8. The fixing device according to claim 7, characterized in that: The adjusting neck (21) is provided with a through hole (210), and the adapter plate (22) is provided with a connecting hole (220) corresponding to the through hole (210); a bolt is passed through the through hole (210) and the connecting hole (220), so that the adjusting neck (21) and the adapter plate (22) are connected, so that the adapter plate (22) can rotate along a third direction; a screw is passed through the screw hole (122) and abutted against the adjusting neck (21) in the wedge-shaped groove (121), so that the adjusting neck (21) is locked and positioned on the crossbeam (12), so that the adjusting neck (21) can move along the first direction.

9. The fixing device according to claim 6 or 7, characterized in that: The fixing plate (23) is provided with a U-shaped groove (231) penetrating the first direction, and the fixing plate (23) on both sides of the U-shaped groove (231) is provided with a plurality of pairs of positioning holes (232) penetrating the first direction.

10. The fixing device according to claim 9, characterized in that: The window patch (3) comprises a central hole (31) and positioning grooves (32) located on both sides of the central hole (31); the central hole (31) is used to expose an imaging observation area; and the window patch (3) is mounted on a plurality of pairs of positioning holes (232) via the positioning grooves (32).

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