Animal experiment device for emotional diseases caused by virus infection and modeling method

Abstract

The invention relates to the technical field of animal scientific experiments, in particular to an animal experiment device for emotional diseases caused by virus infection and a modeling method. The invention provides an animal experiment device for emotional diseases caused by virus infection, which is characterized by comprising a temperature control chassis, an organic glass cover is arranged on the temperature control chassis, a ventilation pipeline is arranged on the organic glass cover, and the ventilation pipeline is communicated with the temperature control chassis. A four-limb fixing device fixed on the temperature control chassis is arranged in the organic glass cover, and a brain stereotaxic instrument is arranged on the four-limb fixing device; and an injector and a shaver are also arranged in the organic glass cover. The method aims at solving the technical problems that in the prior art, a modeling method capable of truly simulating an emotional disease animal model caused by novel coronavirus infection does not exist at present, and a novel coronavirus central infection model cannot be researched in a P2 laboratory.

Description

Technical Field

[0001] The invention relates to the technical field of animal scientific experiments, and in particular to an animal experimental device and a modeling method for emotional diseases caused by viral infection. Background Art

[0002] The spread of the novel coronavirus around the world has had a huge impact on people's physical and mental health. In addition to lung symptoms, novel coronavirus infection can often cause depression, cognitive and other emotional symptoms. However, due to the requirements of the P3 laboratory for novel coronavirus research, there is currently no classic method for modeling animal models of novel coronavirus-induced emotional diseases suitable for P2 laboratories.

[0003] At present, the new coronavirus infection is mostly carried out by establishing a SARS-CoV-2 pseudovirus. This method requires the construction of a SARS-CoV-2 pseudovirus and the screening of mutant strains. The experimental cost is high and the cycle is long. In particular, it cannot truly simulate the disease process of the new coronavirus central infection in vivo, and it cannot simulate the pathogenesis of emotional diseases after central infection. Therefore, the construction of a standard P2 laboratory animal modeling method has become a key issue in the study of emotional diseases caused by new coronavirus infection. At the same time, due to the experimental technical requirements of experimental personnel in the study of central system diseases, the establishment of a standardized device and method for animal models of emotional diseases caused by new coronavirus infection has become an important issue in the in vivo study of emotional diseases caused by new coronavirus infection. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and to provide an animal experimental device and modeling method for emotional diseases caused by viral infection, which aims to solve the technical problems in the prior art that there is currently no modeling method for accurately simulating the animal model of emotional diseases caused by new coronavirus infection, and that it is impossible to study the new coronavirus central infection model in a P2 laboratory.

[0005] To achieve the above-mentioned purpose, the present invention proposes an animal experiment device for emotional diseases caused by viral infection, which is characterized by: comprising a temperature control chassis, a plexiglass cover is arranged on the temperature control chassis, a ventilation duct is arranged on the plexiglass cover, a limb fixing device fixed to the temperature control chassis is arranged in the plexiglass cover, a brain stereotaxic instrument is arranged on the limb fixing device; a syringe and a shaver are also arranged in the plexiglass cover.

[0006] Preferably, a mechanical arm is arranged in the organic glass cover, and a syringe and a shaver are connected to the mechanical arm.

[0007] Preferably, the syringe comprises a micro-syringe and a sterile PE50 tube connected to a Hamilton syringe, and the organic glass cover is fixed to the temperature-controlled chassis by a plurality of locks.

[0008] Preferably, a fluorescent lamp is arranged on the top of the organic glass cover; and an article placement plate is arranged on the temperature control chassis.

[0009] Preferably, a digital control switch is provided on the temperature control chassis, and the digital control switch is used to switch on and off various gases and fluorescent lamps and accurately control the gas volume; the digital control switch can control the movement of the robotic arm and the injection of the syringe.

[0010] Preferably, the limb fixing device comprises a fixing plate, which cannot be higher than the neck of the experimental mouse, and is provided with limb binding straps and a tail binding strap, and the limb binding straps are movably arranged on the fixing plate.

[0011] Preferably, a brain stereotaxic instrument is arranged on the fixing plate, and the brain stereotaxic instrument includes ear fixators, wherein there are two ear fixators, which are symmetrically arranged on both sides of the experimental mouse.

[0012] Preferably, the ear holder comprises a fixing rod, on which a fixing ball is rotatably arranged.

[0013] Preferably, a hollow rod is provided on the fixed ball, and an expansion structure is provided on the hollow rod.

[0014] To achieve the above object, the present invention provides an animal modeling method, the above animal experiment device, First, on the first day, a quiet room was selected to place rats / mice in a plexiglass cover. After inhalation anesthesia, rats and mice were set up in equal groups, and their limbs were fixed on a temperature-controlled chassis. The animal's head was fixed on a brain stereotaxic instrument. The shaver was connected by a digital control device to shave the dorsal side of the experimental animal's skull, and the robotic arm was controlled by the digital control device to go to the object placement tray to take an alcohol cotton ball for disinfection to disinfect the dorsal side of the experimental animal's skull. Then, the location of the cisterna magna was determined by the brain stereotaxic instrument, and the robotic arm was controlled by the digital control device to connect a sterile PE50 tube to a Hamilton syringe to inject the recombinant SARS-CoV-2S protein into the cisterna magna. Secondly, from the 2nd day to the 28th day, the mice were placed in a 1-glass cover and anesthetized by inhalation. The four limbs were fixed on a temperature-controlled chassis, and the temperature-controlled chassis was controlled by a digital control device to tilt the body of the experimental animal downward by 45 degrees. The mechanical arm was connected to a micro-syringe by a digital control device to inject LPS intraperitoneally, and their conditions were observed and recorded every day; Then, one rat / mice were selected as the control group to observe and record their conditions.

[0015] Compared with the prior art, the animal experimental device and modeling method for emotional diseases caused by viral infection provided by the present invention have the following beneficial effects: The present invention controls the opening and closing of the ventilation duct by a digital controller, controls the switch of the fluorescent lamp by a digital controller, controls the inclination of the chassis by a digital controller, controls the mechanical arm to implement the injection of drugs in the cisterna magna and the peritoneal cavity by a digital controller, controls the mechanical arm to implement the shaving of the head of the experimental animal by a digital controller, determines the injection site of the cisterna magna by a brain stereotaxic instrument, and tightly connects the glass cover to the base by a buckle, which can prevent the escape of the experimental animal during the experiment. Thus, under the premise of ensuring biological safety, the standardization and repeatability of the modeling process of the animal model of emotional diseases caused by novel coronavirus infection can be guaranteed, thereby ensuring the safety and accuracy of the experiment.

[0016] The features and advantages of the present invention will be described in detail through embodiments in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a front view of an animal experimental device and modeling method for emotional diseases caused by viral infection in Example 1 of the present invention.

[0018] Figure 2 It is a top view of an animal experimental device and modeling method for emotional diseases caused by viral infection according to Example 1 of the present invention.

[0019] Figure 3 1 is a schematic structural diagram of a smoothing device according to the first embodiment.

[0020] Figure 4 2 is a schematic structural diagram of a limb fixing device according to the first embodiment.

[0021] Figure 5 It is a schematic structural diagram of a common limb fixing device of Example 2.

[0022] Figure 6 It is a schematic structural diagram of a limb fixing device with fixing balls according to the second embodiment.

[0023] Figure 7 It is a schematic structural diagram of the ear holder of the second embodiment.

[0024] Figure 8 It is a schematic diagram of the structure of the roller of the second embodiment.

[0025] Fig. 9 It is a schematic structural diagram of the fixed ball in the second embodiment from a side view.

[0026] Fig.10 It is a schematic diagram of the structure of the head adjustment piece of the second embodiment.

[0027] in: 1-plexiglass cover; 2-ventilation duct; 3-fluorescent lamp; 4-temperature control chassis; 5-limb fixation device; 6-brain stereotaxic instrument; 7-mechanical arm; 8-digital control device; 9-item placement tray; 10-sterile PE; 50-tube connected to Hamilton syringe; 11-micro syringe; 12-shaver; 20-smoothing device; 21-flat plate; 22-ball; 51-semicircular glass cover; 52-injection hole; 53-head injection hole; 54-fixing plate; 55-limb binding strap; 56-tail binding strap; 61-ear fixer; 611-fixing rod; 612-fixing ball; 613-hollow rod; 614-expansion structure; 615-head adjustment plate; 616-telescopic rod; 617-articulated rod; 618-roller. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below through the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0029] In the description of the present invention, it should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or indirectly connected to the other element.

[0030] In the description of the present invention, it should be noted that the terms "center", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invention product is usually placed when in use, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used to distinguish descriptions, and cannot be understood as indicating or implying relative importance. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined. "Several" means one or more, unless otherwise clearly and specifically defined.

[0031] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] Example 1

[0033] See also Figure 1 , Figure 2 The present invention discloses an experimental device for an animal model of an emotional disease caused by a novel coronavirus infection, comprising an organic glass cover 1, a ventilation duct 2, a fluorescent lamp 3, a temperature control chassis 4, a limb fixing device 5, a brain stereotaxic instrument 6, a mechanical arm 7, a digital control device 8, an article placement tray 9, a sterile PE50 tube connected to a Hamilton syringe 10, a micro-injection syringe 11, and a shaver 12. The organic glass cover 1 has a specification of length × width × height: 60cm × 60cm × 60cm, which can meet the modeling experiment of rats and mice. The organic glass cover 1 has 4 locks and can be fixed on the temperature control chassis 4 to prevent the animal from escaping. The ventilation duct 2 and the fluorescent lamp 3 are fixed on the organic glass cover 1. The limb fixing device 5, the brain stereotaxic instrument 6, and the article placement tray 9 are fixed on the temperature control chassis 4. And the switch and movement are controlled by the digital control switch 8. The micro-injection syringe 11 and the shaver 12 are connected and fixed by different branches of the mechanical arm 7, and the switch, movement, and injection are controlled by the digital control switch 8. The organic glass cover 1 contains 4 locks to prevent the experimental animals from escaping, and the temperature control chassis 4 can control the tilt and heat preservation, which is beneficial to the control of the animal body function during the experiment. The mechanical arm 7 is connected to the shaver and the micro-injector and is controlled by a digital switch, which is beneficial to the shaving of the experimental animals and the injection of drugs.

[0034] Working process of the present invention: The modeling method and experimental device of an animal model of emotional diseases caused by novel coronavirus infection of the present invention are as follows: first, a quiet room is selected on the first day to place rats / mice in a plexiglass cover 1 for inhalation anesthesia, and then the limbs are fixed on a temperature-controlled chassis 4, and the animal head is fixed on a brain stereotaxic instrument 6. The shaver 12 is controlled by a digital control device 8 to shave the dorsal side of the skull of the experimental animal, and the mechanical arm 7 is controlled by the digital control device 8 to go to the object placement tray to take an alcohol cotton ball for disinfection to disinfect the dorsal side of the skull of the experimental animal, and then the position of the cerebral cisterna magna is determined by the brain stereotaxic instrument 6, and the mechanical arm 7 is controlled by the digital control device 8 to connect a sterile PE50 tube to connect a Hamilton syringe 10 to inject the recombinant SARS-CoV-2S protein into the cerebral cisterna magna.

[0035] From the 2nd day to the 28th day, the mice were placed in a 1-glass cover and anesthetized by inhalation. The four limbs were fixed on a 4-temperature-controlled chassis. The head of the experimental animal was tilted downward by 45° on the 4-temperature-controlled chassis controlled by an 8-digital control device. The 7-mechanical arm was connected to a microsyringe and LPS was injected intraperitoneally by an 8-digital control device.

[0036] Then, one rat / mice were selected as the control group to observe and record their conditions.

[0037] The organic glass cover 1 can be fixed on the temperature control chassis 4 with a lock, the ventilation duct 2 and the fluorescent lamp 3 are connected to the glass cover, the brain stereotaxic instrument 6, the mechanical arm 7, and the object placement tray are fixed on the temperature control chassis 4, the sterile PE50 tube is connected to the Hamilton syringe 10, the micro syringe 11, and the shaver 12 are connected to the mechanical arm 7, and the ventilation duct 2, the fluorescent lamp 3, and the mechanical arm 7 are connected to the digital control device 8 through wires.

[0038] When the digital control device is used to control the robotic arm to switch on and off the fluorescent lamp and the ventilation duct, when the brain stereotaxic instrument is used to locate the cisterna magna of the experimental animal, when the digital control device is used to control the robotic arm to perform injection into the cisterna magna and intraperitoneal injection, when the digital control device is used to control the robotic arm to connect the shaver to shave the head of the experimental animal, the digital control device can control the tilt of the chassis to facilitate the intraperitoneal injection of the experimental animal.

[0039] The present invention controls the opening and closing of the ventilation duct by a digital controller, controls the switch of the fluorescent lamp by a digital controller, controls the inclination of the chassis by a digital controller, controls the mechanical arm to implement the injection of drugs in the cisterna magna and the peritoneal cavity by a digital controller, controls the mechanical arm to implement the shaving of the head of the experimental animal by a digital controller, determines the injection site of the cisterna magna by a brain stereotaxic instrument, and tightly connects the glass cover to the base by a buckle, which can prevent the escape of the experimental animal during the experiment. Thus, under the premise of ensuring biological safety, the standardization and repeatability of the modeling process of the animal model of emotional diseases caused by novel coronavirus infection can be guaranteed, thereby ensuring the safety and accuracy of the experiment.

[0040] Under anesthesia, the body of the experimental mouse will bend when tied to the limb fixture 5, which will affect the binding and subsequent injection. When the experimental mouse is placed on the limb fixture 5, it is placed supine. The body is smoothed by using a smoothing device 20. The smoothing device 20 includes a flat plate 21, on which a plurality of balls 22 are arranged. The balls are rubber balls, and the distance between the balls 22 is not less than 1 mm.

[0041] The limb fixing device 5 is provided with a semicircular glass cover 51, and injection holes 52 are evenly distributed on the semicircular glass cover 51. The injection holes 52 correspond to each injection acupoint, which is convenient for injection. The semicircular glass cover 51 is provided with a head injection hole 53, and the head injection hole 53 corresponds to the head injection acupoint. Example 2

[0042] In Example 1, the hair of the experimental mouse needs to be shaved. Since the hair is easy to fly everywhere when shaving, a hair suction device needs to be added, and the head needs to be fixed when shaving. Example 2 provides a new limb fixing device and brain stereotaxic device structure. The specific structure is as follows: Referring to 5-6, the limb fixing device 5 of this embodiment includes a fixing plate 54. The fixing plate 54 cannot be higher than the neck of the experimental mouse, and the neck and head must be exposed. The fixing plate 54 is provided with limb binding straps 55 and tail binding straps 56. The limb binding straps 55 can be movably set on the fixing plate 54, which is convenient for fixing experimental mice of different sizes. The tail binding strap 56 can be set to be movable or fixed. The tail of the experimental mouse is generally long, and even if it is fixed, it can cover most large and small mice. The limb binding straps 55 are symmetrical up and down with respect to the center line of the fixing plate 54, and the tail binding strap 56 is just set on the center strap of the fixing plate 54.

[0043] The fixing plate 54 can be raised and lowered and can be rotated, so the experimental mouse can be turned to any angle for easy injection.

[0044] In an alternative embodiment, see Figure 6-10A brain stereotaxic instrument 6 is provided on the fixing plate 54. Figure 5 It is an ordinary brain stereotaxic instrument. When the structure is fixed, if the experimental mouse wakes up and shakes its head, it cannot be fixed well, which damages the hearing of the experimental mouse. In addition, the present invention requires the experimental mouse to be rotated, and the pressure on the ear will change. An ear fixer that does not damage hearing and has a good fixing effect is needed. The brain stereotaxic instrument 6 includes an ear fixer 61. There are two ear fixers 61, which are symmetrically arranged on both sides of the experimental mouse. The ear fixer 61 fixes the head by pressing against the ears of the experimental mouse. The position of the ear fixer 61 can be moved along the height direction of the mouse. After moving, the ear fixer 61 can fix the ear. The ear fixer 61 includes a fixing rod 611, and a fixing ball 612 is rotatably arranged on the fixing rod 611. A hollow rod 613 is arranged on the fixing ball 612, and an expansion structure 614 is arranged on the hollow rod 613. The hollow rod 613 is penetrated by a telescopic rod 616.

[0045] The fixed ball 612 is provided with a head adjustment piece 615. The head adjustment piece 615 can rotate with the fixed ball 612. The head adjustment piece 615 is detachably fixed to the fixed ball 612. The fixed ball 612 is provided with two mounting grooves symmetrically arranged up and down. Generally, one mounting groove can be selected for placement. The head adjustment piece 615 can be mounted on the fixed ball 612 by magnetic attraction. The side of the head adjustment piece 615 close to the experimental mouse is curved, so that the skin will not be scratched when the fixed ball 612 rotates.

[0046] The hollow rod 613 penetrates the ear holder 61 and the two are fixed. The telescopic rod 616 penetrates the hollow rod 613 and the ear holder 61. When the telescopic rod 616 moves in the axial direction, it affects the expansion shape of the expansion structure 614.

[0047] The expansion structure 614 includes an articulated rod 617, one end of which is articulated on the telescopic rod 616, and the middle end is articulated on the end of the hollow rod 613. The other end of the articulated rod 617 is a roller 618. The roller 618 is rotatably arranged on the articulated rod 617, and the rotation direction of the roller 618 is consistent with the rotation direction of the fixed ball 612. The cross-section of the roller 618 presents a runway shape. When the hollow rod 613 extends toward the ear, the articulated rod 617 rotates, and the roller 618 moves axially. During the rotation process, the two tops of the roller 618 may not contact the ear wall. However, arc surfaces are provided on the upper and lower sides of the roller 618, and the arc surfaces can also play a fixing role when they contact the ear canal. When the hollow rod 613 rotates, the roller 618 can also rotate on its own, reducing friction and preventing damage to the ear canal of the experimental mouse.

[0048] During use: the ear holder 61 is moved to the ear of the experimental mouse, and the fixing rod 611 is extended to extend the hollow rod 613 into the ear of the experimental mouse. When the fixing ball 612 is blocked outside the ear, the hollow rod 613 cannot move further into the ear. The telescopic rod 616 begins to telescope toward the inside of the ear, and the originally horizontal hinged rod 617 spreads out in the circumferential direction. The roller 618 on the hinged rod 617 presses against the inside of the ear to achieve fixation.

[0049] Since the ear holder 61 is arranged in the direction of the fixing ball 612 close to the ear, during the rotation of the hollow rod 613, the roller 618 moves circumferentially along the ear, and the head adjustment piece 615 pushes the head to tilt to the left or right or slightly deflect, so as to facilitate injection from different angles.

[0050] The head adjustment piece 615 is configured to be detachable and is placed by a manipulator. The head adjustment piece 615 is configured to be magnetically engaged. The small notch provided on the fixing ball 612 can directly engage the head adjustment piece 615. Since no excessive force is required to adjust the head, a magnetic method can be used. The head adjustment piece 615 of the fixing ball 612 on the bilaterally symmetrical ear fixer 61 is configured to be symmetrical about the center. If one is on top, the other is on the bottom; if one is in front, the other is in the back. In this way, the fixed shape can be maintained when the head is pushed. The above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An animal experimental device for emotional diseases caused by viral infection, characterized in that: The invention comprises a temperature control chassis (4), a plexiglass cover (1) is arranged on the temperature control chassis (4), a ventilation duct (2) is arranged on the plexiglass cover (1), a limb fixing device (5) fixed on the temperature control chassis (4) is arranged inside the plexiglass cover (1), and a brain stereotaxic instrument (6) is arranged on the limb fixing device (5); a syringe and a shaver (12) are also arranged inside the plexiglass cover (1).

2. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 1, characterized in that: A mechanical arm (7) is arranged inside the organic glass cover (1), and a syringe and a shaver (12) are connected to the mechanical arm (7).

3. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 2, characterized in that: The syringe comprises a micro-syringe (11) and a sterile PE50 tube connected to a Hamilton syringe (10), and the organic glass cover (1) is fixed on the temperature control chassis (4) by a plurality of locks.

4. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 3, characterized in that: A fluorescent lamp (3) is arranged on the top of the organic glass cover (1); and an article placement plate is arranged on the temperature control bottom plate (4).

5. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 3, characterized in that: A digital control switch (8) is provided on the temperature control chassis (4), and the digital control switch (8) is used to switch on and off various gases and fluorescent lamps, and accurately control the gas volume; the digital control switch (8) is capable of moving the mechanical arm (7) and injecting the syringe.

6. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 1, characterized in that: The limb fixing device (5) comprises a fixing plate (54), the fixing plate (54) cannot be higher than the neck of the experimental mouse, and a limb binding belt (55) and a tail binding belt (56) are arranged on the fixing plate (54), and the limb binding belt (55) is movably arranged on the fixing plate (54).

7. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 6, characterized in that: A brain stereotaxic instrument (6) is arranged on the fixing plate (54), and the brain stereotaxic instrument (6) comprises an ear fixer (61). There are two ear fixers (61) which are symmetrically arranged on both sides of the experimental mouse.

8. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 7, characterized in that: The ear fixer (61) comprises a fixing rod (611), on which a fixing ball (612) is rotatably arranged.

9. The animal experiment device for emotional diseases caused by viral infection as claimed in claim 8, characterized in that: A hollow rod (613) is arranged on the fixed ball (612), and an expansion structure (614) is arranged on the hollow rod (613).

10. An animal modeling method, characterized in that: Using the animal experiment device according to any one of claims 1 to 9, First, on the first day, a quiet room was selected to place rats / mice in a plexiglass cover (1). After inhalation anesthesia, rats and mice were set up in equal groups, and their limbs were fixed on a temperature-controlled chassis (4). The animal's head was fixed on a stereotaxic apparatus (6). The shaver (12) was controlled by a digital control device (8) to shave the dorsal side of the experimental animal's skull. The digital control device (8) was used to control a robotic arm (7) to go to the object placement tray and take an alcohol cotton ball to disinfect the dorsal side of the experimental animal's skull. The location of the cisterna magna was then determined by the stereotaxic apparatus (6). The digital control device (8) was used to control the robotic arm (7) to connect a sterile PE50 tube to a Hamilton syringe (10) to perform an intracisternal injection of the recombinant SARS-CoV-2S protein. Secondly, from the 2nd day to the 28th day, the mice were placed in a 1-glass cover and anesthetized by inhalation, and the four limbs were fixed on the temperature-controlled chassis (4). The temperature-controlled chassis (4) was controlled by a digital control device (8) to tilt the body of the experimental animal downward by 45 degrees. The digital control device (8) controlled the mechanical arm (7) to connect the micro-injection syringe (11) to perform intraperitoneal injection of LPS, and their conditions were observed and recorded every day; Then, one rat / mice were selected as the control group to observe and record their conditions.

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