Anti-photoaging active composition and application thereof

Abstract

The invention discloses an anti-photoaging active composition which is composed of 75-97 parts of tea extract, 1-19 parts of hexapeptide-9 and 1-8 parts of carnosine, oxidative stress reaction is inhibited by reducing the ROS content in the body according to different proportions of the three active substances, cascade reaction of mitogen activated protein kinase signal channels is further inhibited, and the anti-photoaging active composition has a good anti-photoaging effect. Aging damage caused by ultraviolet radiation is effectively reduced, and a good anti-wrinkle effect is achieved. Meanwhile, the optimal compatibility proportion of the three active matters for exerting the anti-photoaging effect is determined, the anti-photoaging active composition can be used in the field of cosmetics, the formula cost can be reduced, the anti-photoaging effect can be effectively improved, and the anti-photoaging active composition has high market popularization value. The invention also discloses application of the anti-photoaging active composition in preparation of cosmetics for preventing skin photoaging.

Description

Technical Field

[0001] The invention relates to an anti-photoaging active composition and application thereof. Background Art

[0002] Skin aging is generally divided into physiological aging and photoaging. Photoaging is mainly caused by long-term and repeated ultraviolet radiation, which leads to the reduction of dermal collagen fibers, degeneration of elastic fibers and abnormal deposition, and is mainly manifested as loose and rough skin in exposed areas, and deeper and thicker wrinkles. Ultraviolet radiation mainly causes skin photoaging by producing reactive oxygen species (ROS). Excessive ultraviolet radiation can induce the skin to produce a large amount of ROS and reduce the production of intracellular peroxidase and glutathione reductase. ROS can cause telomere shortening, mitochondrial damage, protein isomerization, and cell membrane lipid oxidation. At the same time, ROS-mediated oxidative stress reactions can activate cell signaling pathways such as mitogen-activated protein kinases, promote the expression of matrix metalloproteinases, inhibit collagen synthesis, and form a series of skin manifestations of photoaging damage.

[0003] At present, the effects of individual anti-photoaging active ingredients are single and insufficient, so seeking to use multiple components in combination to reduce costs and improve effects is a current research hotspot. For example, patent CN 113143818 A provides a synergistic effect of two active compositions of wax gourd seed extract and Flammulina velutipes extract in terms of anti-photoaging efficacy. The prior art generally mixes different active ingredients, and verifies the efficacy of the active ingredient ratio through physical and chemical experiments, cell experiments, and consumer tests, but does not fit an optimal compatibility ratio. Therefore, seeking more synergistic composition ratios, optimizing the optimal compatibility ratio, thereby reducing the formula cost and improving the anti-photoaging effect has important application value. Summary of the invention

[0004] The purpose of the present invention is to overcome the defects of the prior art and provide an anti-photoaging active composition, which uses three active substances to reduce the ROS content in the body, thereby inhibiting the oxidative stress reaction, further inhibiting the cascade reaction of the mitogen-activated protein kinase signaling pathway, effectively reducing the aging damage caused by ultraviolet radiation, and having a good anti-wrinkle effect. At the same time, the optimal compatibility ratio of the three active substances to exert the anti-photoaging effect is determined, and the anti-photoaging active composition can be used in the field of cosmetics and has high market promotion value.

[0005] Another object of the present invention is to provide an application of the anti-photoaging active composition.

[0006] A technical solution to achieve the above purpose is: an anti-photoaging active composition, which is composed of 75 to 97 parts of tea extract, 1 to 19 parts of hexapeptide-9 and 1 to 8 parts of carnosine by weight.

[0007] In the above-mentioned anti-photoaging active composition, the mass ratio of the tea extract, hexapeptide-9 and carnosine is preferably 0.87:0.08:0.05.

[0008] The present invention also provides the use of the above-mentioned anti-photoaging active composition in the preparation of cosmetics for preventing skin photoaging.

[0009] In the application of the above-mentioned anti-photoaging active composition, the skin photoaging refers to the manifestation of skin aging damage caused by ultraviolet radiation.

[0010] In the technical scheme of the anti-photoaging active composition of the present invention, tea leaves and their extracts are rich in active substances such as polysaccharides, polyphenols, caffeine, amino acids, etc., and have antioxidant, anti-aging, antibacterial, free radical scavenging and other effects. Hexapeptide-9 is a very stable collagen peptide, and its structure exists simultaneously in the structure of human collagen IV and XVII (two key basement membrane collagens), and has the effects of promoting the differentiation and maturation of epidermal cells, promoting skin regeneration, anti-wrinkle repair, etc. Carnosine is a natural antioxidant component in animal muscle tissue, which is used to regulate the pH of the skin, scavenge free radicals and their metabolites, and can effectively delay skin aging. At the same time, it can also regulate the growth of human fibroblasts and repair aging human cells, and is a good natural skin anti-aging and antioxidant raw material.

[0011] Compared with the prior art, the anti-photoaging active composition of the present invention has the following beneficial effects:

[0012] (1) Tea extract provides antioxidant, anti-aging, antibacterial, and free radical scavenging effects; hexapeptide-9 provides effects such as promoting differentiation and maturation of epidermal cells, promoting skin regeneration, and anti-wrinkle repair; carnosine provides effects such as delaying skin aging and repairing aging human cells;

[0013] (2) The three active ingredients can reduce the ROS content in the body, thereby inhibiting the oxidative stress response, further inhibiting the cascade reaction of the mitogen-activated protein kinase signaling pathway, effectively reducing the aging damage caused by ultraviolet radiation, and having a good anti-wrinkle effect;

[0014] (3) At the same time, the compatibility ratio of the three active ingredients for optimal anti-photoaging efficacy was determined. The anti-photoaging active composition can be used in the cosmetics field to effectively improve the anti-photoaging effect of the formula and has a high market promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Three-dimensional structure diagram constructed for the D-optimal design model;

[0016] Figure 2It is a quantitative diagram of the effect of different compatibility ratios of the anti-photoaging active composition on the ROS content in wild AB type zebrafish;

[0017] Figure 3 It is a quantitative diagram showing the effects of different compatibility ratios of the anti-photoaging active composition on the tail fin area of ​​wild AB type zebrafish;

[0018] Figure 4 This is a phenotypic diagram of the effects of anti-photoaging active compositions in different compatibility ratios on the tail fin area of ​​wild AB type zebrafish.

[0019] Figure 5 The figure is a phenotypic comparison of the effects of a single-component extract and a photoaging active composition in an optimal compatibility ratio on the tail fin area of ​​zebrafish. DETAILED DESCRIPTION

[0020] In order to enable those skilled in the art to better understand the technical solution of the present invention, the specific implementation methods thereof are described in detail below in conjunction with the accompanying drawings:

[0021] In an embodiment of the present invention, the anti-photoaging active composition is composed of 75 to 97 parts of tea extract, 1 to 19 parts of hexapeptide-9 and 1 to 8 parts of carnosine by weight. The tea extract and carnosine are respectively extracted and purified from natural plants and animals. The tea extract, hexapeptide-9 and carnosine can also be commercially available products.

[0022] The different mass ratios among tea extract, hexapeptide-9 and carnosine in the anti-photoaging active composition are shown in the table below.

[0023] Table 1, different mass ratios of three active ingredients:

[0024]

[0025]

[0026] See also Figure 1 , using the D-optimal design mathematical calculation method, under the premise of controlling the total amount of the anti-photoaging active composition to be fixed, the changes in efficacy and activity are correlated through the changes in the compatibility ratio of each component in the anti-photoaging active composition, and a corresponding mathematical model is constructed to explore the relationship between the compatibility ratio of each component in the anti-photoaging active composition and the anti-photoaging efficacy, and a three-dimensional structure diagram is constructed to predict the optimal compatibility ratio range and the optimal compatibility ratio. Figure 1 In the figure, A is the mass ratio of tea extract; B is the mass ratio of hexapeptide-9; and C is the mass ratio of carnosine. From the perspective of numerical modeling, the three components of the anti-photoaging active composition were coordinated and designed, and it was predicted that the anti-photoaging effect was optimal when the compatibility ratio of tea extract: hexapeptide-9: carnosine = 0.87: 0.08: 0.05.

[0027] Then, the anti-photoaging active compositions with different compatibility ratios were biologically evaluated by the ROS inhibition rate and tail fin area contraction in zebrafish, and the anti-photoaging effect of the anti-photoaging active composition with the optimal compatibility ratio was verified.

[0028] 1. Effects of different ratios of anti-photoaging active compositions on ROS content in zebrafish

[0029] See also Figure 2 AB wild-type zebrafish were used during the experiment. Normally developed 2dpf zebrafish larvae were selected and placed in a 12-well cell culture plate, with 10 fish per well. Egg water was added to the blank control group; eggwater was added to the model control group and exposed to UVB for modeling; N-acetyl-L-cysteine ​​solution was added to the positive control group and exposed to UVB for modeling; the sample group was added with a compatible anti-photoaging active composition and exposed to UVB for modeling. Continue to incubate in a 28.5℃ incubator in the dark for 20 hours. After the incubation, wash 3 times, wash off the test culture medium and transfer to a 24-well plate, add 500μL of CM-H2DCFD staining reagent containing 5μg / mL, incubate in the dark for 3 hours, and wash 3 times to wash off the stain. The state of zebrafish embryos and changes in ROS content were observed, and photos were taken with a fluorescent stereo microscope. The quantitative results of the effects of different compatible ratios of photoaging active compositions on ROS content in zebrafish are shown in the figure. Figure 2 shown.

[0030] The experimental results show that: compared with the model control group, the photoaging active composition with different compatibility ratios can significantly inhibit the generation of ROS in zebrafish. And the optimal compatibility ratio group, that is, tea extract: hexapeptide-9: carnosine = 0.87: 0.08: 0.05, has a significantly better inhibitory effect on the generation of ROS in zebrafish than other compatibility ratio groups. The antioxidant effects of the photoaging active composition with different compatibility ratios are shown in Table 2.

[0031] 2. Effects of different ratios of anti-photoaging active compositions on the tail fin area of ​​zebrafish

[0032] See also Figure 3 and Figure 4AB wild-type zebrafish were used during the experiment. Normally developed 2dpf zebrafish fry were selected and placed in a 12-well cell culture plate, with 10 fish per well. Egg water was added to the blank control group; egg water was added to the model control group, and the model was exposed to UVB; snow lotus culture fluid was added to the positive control group, and the model was exposed to UVB; the sample group was added with a compatible ratio of anti-photoaging active composition, and the model was exposed to UVB. Continue to incubate in a 28.5℃ incubator away from light for 20 hours. After the incubation, the state of the zebrafish embryos and the changes in the tail fin area were observed, and photographed with a stereo microscope. The quantitative results of the effects of different compatible ratios of photoaging active compositions on the zebrafish tail fin area are shown in the figure. Figure 3 The phenotypic results are shown in Figure 4 shown.

[0033] The experimental results show that: compared with the model control group, the photoaging active composition with different compatibility ratios can significantly increase the tail fin area of ​​zebrafish. And the optimal compatibility ratio group, that is, tea extract: hexapeptide-9: carnosine = 0.87: 0.08: 0.05, has a significantly better effect on the zebrafish tail fin area than other compatibility ratio groups. The anti-photoaging effects of the photoaging active composition with different compatibility ratios are shown in Table 2.

[0034] Table 2, Antioxidant and anti-photoaging effects of photoaging active compositions with different compatibility ratios:

[0035]

[0036]

[0037] By conducting biological evaluation of the anti-photoaging active composition with different compatibility ratios based on the ROS inhibition rate and tail fin area shrinkage in zebrafish, it can be determined that when tea extract: hexapeptide-9: carnosine = 0.87: 0.08: 0.05, the anti-photoaging active composition has a significantly better inhibitory effect on ROS in zebrafish tail than other compatibility ratio groups, and a significantly better effect on improving the zebrafish tail fin area than other compatibility ratio groups. The above results show that the optimal compatibility ratio group reduces the ROS content in zebrafish, thereby inhibiting the oxidative stress response, further inhibiting the cascade reaction of the mitogen-activated protein kinase signaling pathway, effectively reducing the aging damage caused by ultraviolet radiation, and has a good anti-wrinkle effect.

[0038] Comparative Example

[0039] The difference between the comparative example and the embodiment is that, under the premise of controlling the total amount of the anti-photoaging active composition to be the same, in terms of weight, single component 1 is 100 parts of tea extract, single component 2 is 100 parts of hexapeptide-9, and single component 3 is 100 parts of carnosine. The phenotypic comparison results of the effects of the single-component extract and the photoaging active composition in the optimal compatibility ratio on the caudal fin area of ​​zebrafish are shown in the figure. Figure 5 shown.

[0040] The experimental results show that: compared with the single component group, the photoaging active composition with the optimal compatibility ratio can significantly increase the tail fin area of ​​zebrafish. The anti-photoaging effects of the single component group and the optimal compatibility ratio are shown in Table 3.

[0041] Table 3, anti-light aging effect table of single component group and optimal compatibility ratio:

[0042] name Antioxidant effect (%) Anti-light aging effect (%) Positive control group 63.42 42.57 Single component 1 78.37 70.85 Single component 2 45.77 36.92 Single component 3 52.63 39.14 Optimal compatibility ratio 91.21 93.17

[0043] In summary, the anti-photoaging active composition of the present invention uses three active substances to reduce the ROS content in the body, thereby inhibiting the oxidative stress reaction, further inhibiting the cascade reaction of the mitogen-activated protein kinase signaling pathway, effectively reducing the aging damage caused by ultraviolet radiation, and having a good anti-wrinkle effect. The anti-photoaging active composition can be used in the field of cosmetics, can reduce the formulation cost, effectively improve the anti-photoaging effect, and has a high market promotion value.

[0044] Those skilled in the art should recognize that the above embodiments are only used to illustrate the present invention, and are not intended to limit the present invention. As long as they are within the spirit of the present invention, any changes or modifications to the above embodiments will fall within the scope of the claims of the present invention.

Claims

1. An anti-photoaging active composition, characterized in that: According to weight, it consists of 75 to 97 parts of tea extract, 1 to 19 parts of hexapeptide-9 and 1 to 8 parts of carnosine.

2. The anti-photoaging active composition according to claim 1, characterized in that: The mass ratio of the tea extract, hexapeptide-9 and carnosine is preferably 0.87:0.08:0.

05.

3. Use of the anti-photoaging active composition according to claim 1 in the preparation of cosmetics for preventing skin photoaging.

4. The use of the anti-photoaging active composition according to claim 3, characterized in that: Skin photoaging refers to the manifestation of skin aging damage caused by ultraviolet radiation.

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