Oral care composition as well as preparation method and application thereof
By developing an oral nursing composition containing di metacraifice and copper ions, and mixing it with sodium alginate and water, it forms a hydrogel -like composition to solve the problem of poor efficacy of existing periodontal disease treatment. A better bacteriostatic and anti -inflammatory effects are achieved, which provides new application value for periodontal disease treatment.
Patent Information
- Application Number
- CN202411014912.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-26
- Publication Date
- 2025-01-28
AI Technical Summary
The existing treatment methods for periodontal disease are poorly effective and have great side effects, and lack a safe and effective oral care composition to improve the therapeutic effect of periodontal disease.
Develop a oral nursing composition, including two metformin and copper ions, and mix it with sodium alginate and water by preparing to form a hydrogel -like composition to improve its antibacterial and anti -inflammatory effects.
This composition has significantly improved the effect of bacteriostatic effects by adding di metarium and copper ions. It has a better relieving effect on the inflammation and damage of the periodontal tissue. Treatment of periodontal disease drugs.
Smart Images
Figure CN119367395A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of oral care technology, and in particular to an oral care composition and a preparation method and application thereof. Background Art
[0002] Periodontal disease is a common oral disease, which is mainly manifested by inflammation and destruction of periodontal tissues. At present, the methods for treating periodontal disease mainly include mechanical treatment and drug treatment. However, there are some problems with existing treatment methods, such as poor efficacy and large side effects. Therefore, it is of great significance to develop a new, safe and effective composition for improving the treatment effect of periodontal disease. Summary of the invention
[0003] In view of the above-mentioned deficiencies in the prior art, the purpose of the present application is to provide a safe and effective oral care composition for improving the treatment effect of periodontal disease, and a preparation method and application thereof.
[0004] The technical solution of this application is as follows:
[0005] In a first aspect of the present application, an oral care composition is provided, comprising metformin and copper ions.
[0006] Preferably, the molar ratio of metformin to copper ions is 1:1-1:10.
[0007] Preferably, the oral care composition further comprises sodium alginate and water.
[0008] Preferably, the concentration of the copper ions in the oral care composition is 10-400 μg / mL.
[0009] The second aspect of the present application provides a method for preparing an oral care composition, comprising the steps of:
[0010] Copper chloride, metformin hydrochloride, anhydrous ethanol, and a sodium alginate aqueous solution are provided; the copper chloride is dissolved in the anhydrous ethanol, the metformin hydrochloride is added, and the mixture is heated and dried to obtain copper metformin; the copper metformin is added to the sodium alginate aqueous solution, and the mixture is mixed evenly to obtain the oral care composition.
[0011] Preferably, the molar ratio of the metformin hydrochloride to the copper chloride is 1:1-1:10.
[0012] Preferably, the heating temperature is 50-60°C.
[0013] Preferably, the concentration of metformin copper in the oral care composition is 10-400 μg / mL.
[0014] The third aspect of the present application provides a use of the oral care composition of the present application in the preparation of an oral care product.
[0015] The fourth aspect of the present application provides a use of the oral care composition of the present application in the preparation of a drug for treating periodontal disease.
[0016] Beneficial effects of this application:
[0017] The oral care composition in the present application has a good antibacterial effect by adding metformin and copper ions, and has a relieving effect on inflammation and damage of periodontal tissues. Its effect is better than using metformin alone or using copper ions alone, and has a high application value in the preparation of drugs for treating periodontal disease. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for describing the embodiments are briefly introduced below.
[0019] Figure 1 This is a graph showing the in vitro antibacterial test of the oral care composition provided in the embodiments of the present application on Streptococcus sanguis;
[0020] Figure 2 This is a graph showing the in vitro antibacterial test of the oral care composition provided in the embodiments of the present application against Streptococcus mutans;
[0021] Figure 3 This is a graph showing the in vitro antibacterial test of the oral care composition provided in the examples of the present application on Actinomyces viscosus;
[0022] Figure 4 This is a graph showing the in vitro antibacterial test of the oral care composition provided in the embodiments of the present application on Escherichia coli;
[0023] Figure 5 This is a graph showing the in vitro antibacterial test of the oral care composition provided in the examples of the present application on Staphylococcus aureus. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the drawings and embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the protection scope of the present application. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.
[0025] It should be noted that if there are descriptions involving "first", "second", etc. in the implementation of this application, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance and implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that ordinary technicians in the field can implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this application.
[0026] In a first aspect of an embodiment of the present application, an oral care composition is provided, comprising metformin and copper ions, wherein the metformin may be a compound containing metformin, such as metformin hydrochloride, and the copper ions may be an oxide or compound containing copper ions, such as copper chloride.
[0027] Metformin has anti-inflammatory and antibacterial effects, and copper ions have antibacterial and wound healing effects. The oral care composition prepared by mixing metformin and copper ions has an antibacterial effect that can effectively relieve inflammation of periodontal tissues and promote the recovery of periodontal tissue damage compared to the use of metformin alone or copper ions alone.
[0028] In some embodiments, the molar ratio of metformin to copper ions is 1:1-1:10. For example, the molar ratio of metformin to copper ions is 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 or 1:10, etc. By controlling the molar ratio of metformin to copper ions, the oral care composition can achieve the best relief effect on periodontal tissue inflammation and damage. In some embodiments, metformin and copper ions can be metformin copper.
[0029] Furthermore, in some embodiments, the oral care composition further comprises sodium alginate and water. Metformin and copper are mixed with the sodium alginate aqueous solution to prepare the oral care composition into a hydrogel. Using sodium alginate as a carrier, the active ingredients in the oral care composition can be better attached to the oral cavity, slowly released, and continuously exert anti-inflammatory, antibacterial and wound healing effects, relieve inflammation of periodontal tissues, and promote the recovery of periodontal tissue damage.
[0030] In some embodiments, the concentration of copper in the oral care composition is 10-400 μg / mL. For example, the concentration of copper in the oral care composition is 10 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL, 250 μg / mL, 300 μg / mL, 350 μg / mL, or 400 μg / mL, etc. The concentration of metformin in the oral care composition is 20-800 μg / mL. For example, the concentration of metformin in the oral care composition is 20 μg / mL, 50 μg / mL, 100 μg / mL, 200 μg / mL, 300 μg / mL, 400 μg / mL, 500 μg / mL, 600 μg / mL, 700 μg / mL, or 800 μg / mL, etc.
[0031] A second aspect of the present invention provides a method for preparing an oral care composition, comprising the steps of:
[0032] Provide copper chloride, metformin hydrochloride, anhydrous ethanol, and sodium alginate aqueous solution;
[0033] Dissolving copper chloride in anhydrous ethanol, adding metformin hydrochloride, heating and drying to obtain metformin copper;
[0034] Copper metformin is added into the sodium alginate aqueous solution and mixed evenly to obtain an oral care composition.
[0035] In some embodiments, the mass ratio of metformin hydrochloride to copper chloride is 2:1.
[0036] In some embodiments, the heating temperature is 50-60° C. For example, the heating temperature is 50° C., 51° C., 52° C., 53° C., 54° C., 55° C., 56° C., 57° C., 58° C., 59° C. or 60° C., etc. Preferably, the heating temperature is 55° C.
[0037] In some embodiments, the concentration of metformin copper in the oral care composition is 10-400 μg / mL. For example, the concentration of metformin copper in the oral care composition is 10 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL, 250 μg / mL, 300 μg / mL, 350 μg / mL, 400 μg / mL, 450 μg / mL or 500 μg / mL, etc.
[0038] In some embodiments, after adding copper metformin to the sodium alginate aqueous solution, an appropriate amount of 10% copper chloride solution may be added. Copper metformin may undergo a cross-linking reaction with the sodium alginate solution to form a hydrogel. However, when the amount of copper metformin added is not enough to completely cross-link the sodium alginate solution, 10% copper chloride may be slowly added to the mixed solution of copper metformin and sodium alginate while stirring to obtain a hydrogel. Subsequently, the residual copper chloride may be washed off with deionized water to obtain an oral care composition.
[0039] A third aspect of the embodiments of the present application provides a use of the above-mentioned oral care composition in the preparation of oral care products.
[0040] A fourth aspect of the embodiments of the present application provides a use of the above-mentioned oral care composition in the preparation of a drug for treating periodontal disease.
[0041] The following will provide further explanation through specific examples.
[0042] Example 1
[0043] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.02 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0044] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 10 μg / mL copper metformin, thereby obtaining an oral care composition.
[0045] Example 2
[0046] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.02 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0047] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 50 μg / mL copper metformin, thereby obtaining an oral care composition.
[0048] Example 3
[0049] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.02 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0050] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 100 μg / mL copper metformin, thereby obtaining an oral care composition.
[0051] Example 4
[0052] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.02 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0053] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 200 μg / mL copper metformin, thereby obtaining an oral care composition.
[0054] Example 5
[0055] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.02 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0056] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0057] Example 6
[0058] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.01 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0059] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0060] Example 7
[0061] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.03 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0062] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0063] Example 8
[0064] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.05 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0065] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0066] Example 9
[0067] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.07 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0068] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0069] Example 10
[0070] Take 0.01 mol of copper chloride in 100 ml of anhydrous ethanol and stir until it is completely dissolved, add 0.1 mol of metformin hydrochloride, heat and stir until it is completely dissolved, and continue to maintain 55°C until the liquid evaporates and blue crystals are precipitated. Wash the product with anhydrous ethanol and dry it to obtain metformin copper.
[0071] Copper metformin and an aqueous sodium alginate solution are mixed in a certain proportion, and stirred to obtain a hydrogel containing 400 μg / mL copper metformin, thereby obtaining an oral care composition.
[0072] Comparative Example 1
[0073] Metformin and sodium alginate aqueous solution were mixed in a certain proportion and stirred to obtain a hydrogel containing 400 μg / mL metformin.
[0074] Comparative Example 2
[0075] The copper chloride and sodium alginate aqueous solution were mixed in a certain proportion and stirred to obtain a hydrogel containing 400 μg / mL copper chloride.
[0076] Test Example 1
[0077] The oral care compositions prepared in Examples 1-5 and the hydrogels prepared in Comparative Examples 1 and 2 were used to conduct in vitro antibacterial tests on Streptococcus sanguis (S.sg), Streptococcus mutans (Sm), Actinomyces viscosus (Av), Escherichia coli (K12), and Staphylococcus aureus (SA). The specific test method is as follows:
[0078] The tested fungi were cultured at 37°C, 220 rpm, overnight for about 16 hours. The bacterial OD was measured. 600 The value is about 1, and it is inoculated into fresh culture medium at a ratio of 1:50 (MRS medium is used for Lactobacillus Ls, BHI liquid medium is used for Streptococcus sanguis Ssg, Streptococcus salivarius Ssl, Actinomyces viscosus Av, and Streptococcus mutans Sm, and LB medium is used for Escherichia coli K12 MG1655 and Staphylococcus aureus ATCC25923).
[0079] Add 1 mL of fresh culture medium / drug of corresponding concentration (prepared with the same culture medium) and 10 μL of bacterial solution to 1.5 mL EP tubes and mix well. Then dispense into 96-well plates, 100 μL per well (set up 8 groups for each bacteria, 3 replicates per group). Use an ELISA reader to measure the OD value of each well on the 96-well plate at 30 °C, and determine the bacterial growth status based on the OD value.
[0080] Test results such as Figure 1-5 As shown. Figure 1-5 It can be seen that the oral care compositions prepared in Examples 1-5 have obvious antibacterial effects on Streptococcus sanguis (S.sg), Streptococcus mutans (Sm), Actinomyces viscosus (Av), Escherichia coli (K12), and Staphylococcus aureus (SA), and can continue to inhibit bacteria within 12 hours. In addition, the oral care composition prepared in Example 5 has an antibacterial effect on Streptococcus sanguis (S.sg), Streptococcus mutans (Sm), and Actinomyces viscosus (Av) that is significantly better than that of the hydrogels prepared in Comparative Examples 1 and 2, indicating that the oral care composition containing metformin and copper in the present application has a better antibacterial effect on some bacteria than the antibacterial effect of the hydrogel containing only metformin or only copper.
[0081] In summary, the oral care composition in the present application has a good antibacterial effect by adding metformin and copper, and has a relieving effect on inflammation and damage of periodontal tissues. Its effect is better than using metformin alone or using copper alone, and has a high application value in the preparation of drugs for treating periodontal disease.
[0082] It should be understood that the application of the present application is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to this application.
Claims
1. An oral care composition, characterized in that The oral care composition includes metformin and copper ions.
2. The oral care composition according to claim 1, characterized in that The molar ratio of metformin to copper ions is 1:1-1:
10.
3. The oral care composition according to claim 2, characterized in that The oral care composition also includes sodium alginate and water.
4. The oral care composition according to claim 3, characterized in that The concentration of the copper ions in the oral care composition is 10-400 μg / mL.
5. A method for preparing an oral care composition, characterized in that: Includes steps: Provide copper chloride, metformin hydrochloride, anhydrous ethanol, and sodium alginate aqueous solution; Dissolving the copper chloride in the anhydrous ethanol, adding the metformin hydrochloride, heating and drying to obtain metformin copper; The metformin copper is added into the sodium alginate aqueous solution and mixed evenly to obtain the oral care composition.
6. The preparation method according to claim 5, characterized in that: The molar ratio of the metformin hydrochloride to the copper chloride is 1:1-1:
10.
7. The preparation method according to claim 5, characterized in that: The heating temperature is 50-60°C.
8. The preparation method according to claim 5, characterized in that: The concentration of the metformin copper in the oral care composition is 10-400 μg / mL.
9. Use of the oral care composition according to any one of claims 1 to 4 in the preparation of oral care products.
10. Use of the oral care composition according to any one of claims 1 to 4 in the preparation of a drug for treating periodontal disease.