Veterinary motherwort biochemical mixture and preparation method thereof

Abstract

The invention discloses a veterinary motherwort biochemical mixture and a preparation method thereof, and belongs to the field of livestock pharmaceutical preparations, the veterinary motherwort biochemical mixture comprises the following components by weight: 6-10 parts of motherwort, 3-6 parts of Chinese angelica, 3-6 parts of Ligusticum wallichii, 3-6 parts of peach kernel, 3-6 parts of Radix Astragali, 3-6 parts of baked ginger and 3-6 parts of honey-fried licorice root. The invention overcomes the defects that the existing motherwort biochemical mixture as a veterinary drug is incomplete in drug effect and slow in effect.

Description

Technical Field

[0001] The invention belongs to the field of livestock drug preparations, and in particular relates to a veterinary Leonurus biochemical mixture and a preparation method thereof. Background Art

[0002] During pregnancy, especially in the later stages of pregnancy, the rapid development of the fetus will consume a large amount of the mother's Qi and blood. Childbirth consumes Qi and blood. Postpartum lactation mobilization and lactation will both consume the body's Qi and blood. In addition, the residual blood and turbid fluid retained in the uterus after delivery will cause uterine contraction weakness, which can easily lead to postpartum diseases in postpartum animals with pathological characteristics of "many blood stasis, many deficiencies, and a mixture of deficiency and excess", or the so-called postpartum syndrome.

[0003] Postpartum female animals often experience abdominal pain due to retained lochia and internal blood stasis; Qi and blood deficiency, insufficient labor force, resulting in retained placenta and internal blood stasis; insufficient labor force and weak Qi and blood leading to incomplete uterine involution or even uterine and vaginal prolapse; coupled with poor feeding and management, exposure to wind and cold leading to postpartum cold; or because of the cold entering the body and turning into heat, or exposure to heat evil, which can also easily cause ewes to experience postpartum fever (including postpartum infection and febrile diseases such as postpartum vaginitis and endometritis), yellow milk (jaundice, mastitis), lack of milk or little milk in postpartum female animals, postpartum Qi and blood deficiency, gastrointestinal paralysis leading to less food, no food and postpartum constipation; postpartum Qi and blood weakness leading to no estrus, infertility and difficulty in conceiving. From the perspective of modern medicine, the main reasons for postpartum diseases in female animals are damage to the birth canal, decreased immune function caused by delivery stress, drastic changes in hormones after delivery, and factors of the body itself such as lactation mobilization and lactation, as well as poor feeding and management. These diseases include postpartum infection (puerperal fever), postpartum paralysis, lack of milk / little milk, postpartum constipation, postpartum indigestion (little food / no food after delivery), incomplete involution of the uterus after delivery (lochia not exhausted), postpartum endometritis, postpartum estrus, mastitis and other diseases. The most common postpartum diseases in ewes include little food / no food after delivery; lochia not exhausted (lochia should be exhausted within a week under normal circumstances, and ewes that still discharge lochia beyond this time are suffering from lochia not exhausted); little milk / no milk; dampness in the lower jiao / damp heat in the lower jiao; internal invasion of heat and toxicity; postpartum paralysis and other symptoms. Among them, modern medicine often refers to the "endometritis, postpartum mastitis and lack of milk" suffered by postpartum livestock as the postpartum "triad".

[0004] In the prevention and treatment of postpartum "triad syndrome", modern medicine often uses antibiotics, hormones and other drugs for prevention or treatment. However, with the stricter control of antibiotics, hormones and other drugs and their residues, and the deepening implementation of the awareness of "banning and reducing antibiotics", the use of these drugs has been questioned, abandoned or used with special caution. Therefore, there is an urgent need for a better Chinese medicine to prevent and treat postpartum lochia, promote uterine contraction and involution, prevent and treat postpartum infection, and have a broad spectrum of drugs without residual harmful substances such as hormones. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a veterinary Leonurus biochemical mixture and a preparation method thereof in view of the deficiencies of the above-mentioned prior art, thereby overcoming the defects of the existing Leonurus biochemical mixture as a veterinary medicine, that is, the efficacy is not comprehensive and the effect is slow.

[0006] The technical solution adopted by the present invention is: a veterinary Leonurus biochemical mixture, which comprises the following components by weight: 6-10 parts of Leonurus japonicus, 3-6 parts of Angelica sinensis, 3-6 parts of Chuanxiong, 3-6 parts of peach kernel, 3-6 parts of Astragalus membranaceus, 3-6 parts of roasted ginger and 3-6 parts of roasted licorice.

[0007] In one embodiment, the veterinary Leonurus biochemical mixture includes the following components by weight: 4 parts of Leonurus herb, 2 parts of Angelica sinensis, 2 parts of Ligusticum chuanxiong, 2 parts of peach kernel, 1 part of Astragalus membranaceus, 2 parts of roasted ginger and 2 parts of roasted licorice.

[0008] In one embodiment, the veterinary Leonurus biochemical mixture includes the following components by weight: 8 parts of Leonurus herb, 4 parts of Angelica sinensis, 4 parts of Ligusticum chuanxiong, 4 parts of peach kernel, 2 parts of Astragalus, 4 parts of Rhizoma Zingiberis Preparata and 4 parts of Radix Glycyrrhizae Preparata.

[0009] The present invention also discloses a method for preparing a veterinary Leonurus biochemical mixture, which is specifically as follows:

[0010] Step 1, weigh the raw materials required for the veterinary Leonurus biochemical mixture according to the weight parts and set aside;

[0011] Step 2: Grind the astragalus into medium powder, extract with 10 times the volume of calcium oxide aqueous solution, reflux and concentrate to obtain an astragalus extract;

[0012] Step 3, pulverize angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction with 5 times volume of ethanol, evaporate the ethanol, and obtain a mixed extract;

[0013] Step 4, crushing the motherwort, performing Soxhlet extraction with 10 times the volume of ethanol, and evaporating the ethanol to obtain a motherwort extract;

[0014] Step 5, respectively mixing the astragalus extract, the mixed extract and the motherwort extract obtained in step 2 to step 5, adding an equal amount of distilled water, stirring evenly, filtering, and obtaining a mixed medicinal solution;

[0015] Step 6: sampling and testing the mixed liquid medicine obtained in step 5, and after passing the test, filling and sterilizing to obtain the veterinary Leonurus biochemical mixture.

[0016] In one embodiment, the step 2 is specifically as follows:

[0017] The astragalus was crushed into medium powder, soaked in 10 times the volume of 1% calcium oxide aqueous solution for 30 minutes, refluxed and extracted twice, each time for 1 hour, the filtrates were combined and concentrated to obtain the astragalus extract.

[0018] In one embodiment, the step three is as follows:

[0019] Grind angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction multiple times with 5 times volume of 80% ethanol, each time for 5-7 minutes, combine the multiple flash extractions, concentrate under reduced pressure to recover ethanol, and obtain a mixed extract.

[0020] In one embodiment, the concentration of ethanol during Soxhlet extraction is 90%-95%.

[0021] In one of the embodiments, in step five, in every 300 ml of the mixed medicinal liquid, the raw drug amount of Astragalus is 20 g, the raw drug amount of Leonurus japonicus is 80 g, the raw drug amount of Angelica sinensis is 40 g, the raw drug amount of Ligusticum chuanxiong is 40 g, the raw drug amount of peach kernel is 40 g, the raw drug amount of Zingiber officinale is 40 g, and the raw drug amount of Licorice root is 40 g.

[0022] The beneficial effects of the present invention are:

[0023] 1. The mixture is a Chinese medicine compatibility formula with Leonurus japonicus as the main drug, Angelica sinensis, Chuanxiong, Peach Kernel and Astragalus membranaceus as the assistant drugs, Paojiang as the adjuvant drug, and Zhigancao as the guiding drug. It significantly improves the original Leonurus japonicus biochemical mixture in treating a series of postpartum syndromes such as lochia retention, blood stasis and abdominal pain, and qi and blood deficiency in female livestock. The combination of these drugs has the effects of promoting blood circulation, removing blood stasis, warming the meridians and relieving pain.

[0024] 2. The present invention is a pure Chinese medicine preparation, does not contain hormone components, has no toxic side effects, has good efficacy, and is quick in effect. It has the functions of exciting the uterus, promoting uterine contraction, improving blood flow rheology, and regulating cardiovascular function; it has good effects on promoting postpartum uterine involution, repairing postpartum endometrium, anti-inflammatory, enhancing immunity, and antibacterial; it has a significant effect on the prevention and treatment of postpartum diseases;

[0025] 3. The veterinary Yimu biochemical mixture of the present invention carries out targeted extraction of Astragalus membranaceus, and can purify the effective components, thereby significantly improving the prevention and treatment effect of the veterinary Yimu biochemical mixture on postpartum syndrome in female livestock;

[0026] 4. The raw materials of the mixture are all common substances with wide sources, low prices and low production costs. It can effectively prevent and treat postpartum syndrome in female livestock and can be applied to a variety of animals, suitable for large-scale promotion and application;

[0027] 5. The preparation method of the veterinary Leonurus biochemical mixture of the present invention is simple and reliable, and is suitable for large-scale production of the veterinary Leonurus biochemical mixture. DETAILED DESCRIPTION

[0028] The present invention will be further described in detail below with reference to specific embodiments.

[0029] Embodiment 1:

[0030] The invention discloses a veterinary Leonurus biochemical mixture, which comprises the following components by weight: 4 parts of Leonurus herb, 2 parts of Angelica sinensis, 2 parts of Chuanxiong rhizome, 2 parts of peach kernel, 1 part of Astragalus membranaceus, 2 parts of roasted ginger and 2 parts of roasted liquorice root.

[0031] The present invention also discloses a method for preparing a veterinary Leonurus biochemical mixture, which is specifically as follows:

[0032] Step 1, weigh the raw materials required for the veterinary Leonurus biochemical mixture according to the weight parts and set aside;

[0033] Step 2: Grind the astragalus into medium powder, extract with 10 times the volume of calcium oxide aqueous solution, reflux and concentrate to obtain an astragalus extract;

[0034] Step 3, pulverize angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction with 5 times volume of ethanol, evaporate the ethanol, and obtain a mixed extract;

[0035] Step 4, crushing the motherwort, performing Soxhlet extraction with 10 times the volume of ethanol, and evaporating the ethanol to obtain a motherwort extract;

[0036] Step 5, respectively mixing the astragalus extract, the mixed extract and the motherwort extract obtained in step 2 to step 5, adding an equal amount of distilled water, stirring evenly, filtering, and obtaining a mixed medicinal solution;

[0037] Step 6: sampling and testing the mixed liquid medicine obtained in step 5, and after passing the test, filling and sterilizing to obtain the veterinary Leonurus biochemical mixture.

[0038] In this embodiment, the step 2 is specifically as follows:

[0039] The astragalus was crushed into medium powder, soaked in 10 times the volume of 1% calcium oxide aqueous solution for 30 minutes, refluxed and extracted twice, each time for 1 hour, the filtrates were combined and concentrated to obtain the astragalus extract.

[0040] In this embodiment, the step three is as follows:

[0041] Grind angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction multiple times with 5 times volume of 80% ethanol, each time for 5-7 minutes, combine the multiple flash extractions, concentrate under reduced pressure to recover ethanol, and obtain a mixed extract.

[0042] In this embodiment, the concentration of ethanol during Soxhlet extraction is 90%-95%.

[0043] Embodiment 2:

[0044] The invention discloses a veterinary Leonurus biochemical mixture, which comprises the following components by weight: 8 parts of Leonurus herb, 4 parts of Angelica sinensis, 4 parts of Chuanxiong rhizome, 4 parts of peach kernel, 2 parts of Astragalus membranaceus, 4 parts of roasted ginger and 4 parts of roasted liquorice root.

[0045] The present invention also discloses a method for preparing a veterinary Leonurus biochemical mixture, which is specifically as follows:

[0046] Step 1, weigh the raw materials required for the veterinary Leonurus biochemical mixture according to the weight parts and set aside;

[0047] Step 2: Grind the astragalus into medium powder, extract with 10 times the volume of calcium oxide aqueous solution, reflux and concentrate to obtain an astragalus extract;

[0048] Step 3, pulverize angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction with 5 times volume of ethanol, evaporate the ethanol, and obtain a mixed extract;

[0049] Step 4, crushing the motherwort, performing Soxhlet extraction with 10 times the volume of ethanol, and evaporating the ethanol to obtain a motherwort extract;

[0050] Step 5, respectively mixing the astragalus extract, the mixed extract and the motherwort extract obtained in step 2 to step 5, adding an equal amount of distilled water, stirring evenly, filtering, and obtaining a mixed medicinal solution;

[0051] Step 6: sampling and testing the mixed liquid medicine obtained in step 5, and after passing the test, filling and sterilizing to obtain the veterinary Leonurus biochemical mixture.

[0052] In this embodiment, the step 2 is specifically as follows:

[0053] The astragalus was crushed into medium powder, soaked in 10 times the volume of 1% calcium oxide aqueous solution for 30 minutes, refluxed and extracted twice, each time for 1 hour, the filtrates were combined and concentrated to obtain the astragalus extract.

[0054] In this embodiment, the step three is as follows:

[0055] Grind angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction multiple times with 5 times volume of 80% ethanol, each time for 5-7 minutes, combine the multiple flash extractions, concentrate under reduced pressure to recover ethanol, and obtain a mixed extract.

[0056] In this embodiment, the concentration of ethanol during Soxhlet extraction is 90%-95%.

[0057] In this embodiment, in step five, in every 300 ml of mixed medicinal liquid, the raw drug amount of Astragalus is 20 g, the raw drug amount of Leonurus is 80 g, the raw drug amount of Angelica is 40 g, the raw drug amount of Ligusticum chuanxiong is 40 g, the raw drug amount of peach kernel is 40 g, the raw drug amount of Zingiber officinale is 40 g and the raw drug amount of Radix Glycyrrhizae Radix is ​​40 g.

[0058] In the above two embodiments, the astragalus root is sweet in taste and slightly warm in nature; it has the effects of replenishing qi and strengthening the exterior, promoting diuresis, strengthening the heart, lowering blood pressure, resisting bacteria, detoxifying, draining pus, promoting tissue regeneration, strengthening capillary resistance, stopping sweating and acting like sex hormone. The herb Leonurus is pungent in nature, bitter in taste, and has the effects of activating blood circulation and removing blood stasis, and promoting new blood circulation; the angelica is sweet and pungent in taste and warm in nature; it enters the liver, heart and spleen, and has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation; the ligusticum chuanxiong is pungent in taste and warm in nature; it enters the liver, gallbladder and pericardium meridians, and has the effects of activating blood circulation and promoting qi, and dispelling wind and relieving pain; the peach kernel is bitter, sweet and neutral in taste, enters the heart, liver and large intestine meridians, and has the effects of activating blood circulation and removing blood stasis, moistening the intestines and relieving constipation, and relieving cough and asthma; the roasted ginger is pungent and hot in nature, enters the spleen, stomach and kidney meridians, and has the effects of warming the meridians and stopping bleeding, and warming the middle and relieving pain; the roasted liquorice is sweet and neutral in nature, enters the spleen, lung and stomach meridians, and has the effects of sweetness, slowing down and tonifying the middle; all the medicines are used together to achieve the effects of activating blood circulation and removing blood stasis, and warming the meridians and relieving pain. The results of modern pharmacological research show that: stachydrine and leonurine contained in motherwort, angelica polysaccharides and ferulic acid contained in angelica and Chuanxiong, and amygdalin contained in peach kernel all have the functions of stimulating the uterus, promoting uterine contraction, improving blood rheology, and regulating cardiovascular function.

[0059] Effect verification:

[0060] 1. Identification of Astragaloside IV:

[0061] Take 30ml of the veterinary Yimu biochemical mixture sample prepared in Example 2, shake and extract twice with n-butanol saturated with water, 20ml each time, combine the n-butanol solution, wash once with 15ml of ammonia test solution, and then wash once with 15ml of water saturated with n-butanol. Take the n-butanol solution and evaporate it to dryness, add 1ml of methanol to dissolve the residue, as the solution of the test sample; take another astragaloside reference substance, add methanol to make a solution containing 1mg per 1ml, as the reference substance solution. According to the thin layer chromatography method (Chinese Veterinary Pharmacopoeia 2020 Edition Part II), take 5μl of each of the above two solutions and spot them on the same silica gel G thin layer plate with sodium carboxymethyl cellulose as the adhesive, and use the lower layer solution of chloroform-methanol-water (13:7:2) as the developing agent, develop, take out, dry, spray with 10% sulfuric acid ethanol solution, and heat until the spots are clearly colored. In the chromatogram of the test sample, a spot of the same color appears at the corresponding position in the chromatogram of the reference sample; when examined under ultraviolet light (365nm), a fluorescent spot of the same color appears.

[0062] 2. Identification of Stachydrine Hydrochloride:

[0063] Take 20ml of the veterinary Yimu biochemical mixture sample prepared in Example 1, evaporate it on a water bath until it is nearly dry, add 5ml of ethanol to the residue to dissolve it, filter it, and concentrate the filtrate to 2ml as the test solution. Take another reference substance of stachydrine hydrochloride, add ethanol to make a solution containing 5mg per 1ml as a reference solution. According to the thin layer chromatography method (Part II of the 2020 edition of the Chinese Pharmacopoeia of Veterinary Medicine), 10μl of each of the above two solutions are taken and spotted on the same silica gel G thin layer plate, with n-butanol-hydrochloric acid-water (4:1:0.5) as the developing agent, develop it, take it out, dry it, spray it with dilute potassium bismuth iodide test solution, and heat it at 105°C until the spots are clearly colored. In the test sample chromatogram, spots of the same color appear at the corresponding position of the reference chromatogram.

[0064] The properties of the veterinary Leonurus biochemical mixture provided in Example 2 are: light orange-yellow to brown-yellow liquid; fragrant and slightly sweet.

[0065] The inspection of the veterinary Leonurus biochemical mixture provided in Example 2 meets the following standards:

[0066] (1) Relative density should not be less than 1.04 (Chinese Veterinary Pharmacopoeia 2020 Edition Part II);

[0067] (ii) pH value, which should be between 5.0 and 7.0 (Chinese Pharmacopoeia of Veterinary Medicine 2020 Edition, Part II);

[0068] (3) Others must comply with the relevant provisions under the mixture (Part II of the Chinese Pharmacopoeia of Veterinary Medicine 2020 Edition).

[0069] 3. Verification results:

[0070] The functions and indications of the veterinary Leonurus biochemical mixture provided in Example 2 are: promoting blood circulation and removing blood stasis, warming the meridians and relieving pain, and tonifying the middle and replenishing qi; it is used for postpartum lochia, abdominal pain due to blood stasis, and physical weakness and weak qi.

[0071] In order to verify the preventive and therapeutic effect of the veterinary Yimu biochemical mixture on a series of syndromes such as postpartum lochia retention and recovery in animals, the following experiments were conducted:

[0072] During pregnancy, especially in the later stages of pregnancy, the rapid development of the fetus will consume a large amount of the mother's Qi and blood. Childbirth consumes Qi and blood. Postpartum lactation mobilization and lactation will both consume the body's Qi and blood. In addition, the residual blood and turbid fluid retained in the uterus after delivery will cause uterine contraction weakness, which can easily lead to postpartum diseases in postpartum animals with pathological characteristics of "many blood stasis, many deficiencies, and a mixture of deficiency and excess", or the so-called postpartum syndrome.

[0073] Postpartum recovery of sows has always been a major problem that plagues the pig farming industry. The experiment selected 80 sows to be born, and each sow to be born was randomly divided into a control drug group and a drug administration group, with 40 in each group, and there was no significant difference in body size between the groups. After the sows gave birth, all sows in the drug administration group were given the medicine of Example 2 respectively, and all sows in the control drug group were given the control drug. The control drug selected the Yimu Biochemical Mixture prepared according to the pharmacopoeia. The drug administration group selected the Yimu Biochemical Mixture for Animal Use, which was administered orally at 1 ml per 1.5 kg of body weight, twice a day, for 7 consecutive days. The control drug Yimu Biochemical Mixture was administered in the same way for 7 consecutive days. During the administration period and within 20 days of drug withdrawal, the conditions such as breast milk, feeding, and lochia discharge of the sows were observed. The results show that in the drug administration group, most of the sows can quickly expel lochia, eat and drink water to return to normal, and have a good mental state after administration, and the effect of the drug administration group is much better than that of the control group; a small number of sows in the control drug group can quickly expel lochia, eat and drink water to return to normal, and have a good mental state. After stopping the drug and continuing to observe for 20 days, there is still postpartum syndrome; it can be seen that the drug in Example 2 has a good and long-term preventive effect on the postpartum recovery of sows, and the Yimu Biochemical Mixture prescribed in the pharmacopoeia has no such effect. The results are shown in Table 1. The control drug did not have a good treatment for postpartum syndrome in some sows, and the treatment was not thorough; the effect of treating postpartum lochia was not as strong as that of the drug administration group, and the pharmacological effect was significantly better than that of the control drug group.

[0074] Table 1 Comparison of sows treated with medication and control groups

[0075] Group Number of experimental animals Average days to complete lochia discharge Number of postpartum syndrome Average weight after postpartum test kg Drug group 40 2.9 4 +1.8 Control group 40 4.2 11 +0.4

[0076] Ewes also often experience abdominal pain caused by lochia not being exhausted and blood stasis after delivery; Qi and blood are weak, and the placenta is not down due to insufficient production force, and blood stasis is also seen. The experiment selected 100 ewes to be born, and each of the ewes to be born was randomly divided into a control drug group and a drug administration group, with 50 in each group, and there was no significant difference in the size of the body between the groups. After the ewe gave birth, all the ewes in the drug administration group were given the medicine of embodiment 2 respectively, and all the ewes in the control drug group were given the control drug. The control drug selected the Yimu biochemical mixture prepared by the pharmacopoeia. The drug administration group selected the Yimu biochemical mixture for animals, and it was administered by gavage of 1ml per 1.5 kg body weight, 2 times a day, for 7 consecutive days. The control drug Yimu biochemical mixture was administered in the same way for 7 consecutive days. During the administration period and within 20 days of drug withdrawal, the conditions such as breast milk, feeding, and lochia discharge of the ewe were observed. The results show that in the drug administration group, most of the ewes can quickly expel lochia, eat and drink water to return to normal, and have a good mental state after administration, and the effect of the drug administration group is much better than that of the control group; a small number of ewes in the control drug group can quickly expel lochia, eat and drink water to return to normal, and have a good mental state. After stopping the drug and continuing to observe for 20 days, there is still postpartum syndrome; it can be seen that the drug of Example 2 has a good and long-term preventive effect on the postpartum recovery of ewes, and the Yimu Biochemical Mixture prescribed in the pharmacopoeia has no such effect. The results are shown in Table 2. The control drug did not have a good treatment for postpartum syndrome in some ewes, and the treatment was not thorough; the effect of treating postpartum lochia was not as strong as that of the drug administration group, and the pharmacological effect was significantly better than that of the control drug group.

[0077] Table 2 Experimental comparison of ewe medication group and control group

[0078] Group Number of experimental animals Average days to complete lochia discharge Number of postpartum syndrome Average weight after postpartum test kg Drug group 50 3.2 6 +2.2 Control group 50 4.8 15 +0.6

[0079] The veterinary Yimu Biochemical Combination is a representative prescription for treating postpartum syndrome in female livestock, and is mainly used to treat a series of postpartum syndromes such as postpartum lochia retention, abdominal pain caused by blood stasis, and insufficient qi and blood in female livestock. The veterinary Yimu Biochemical Combination is pungent and bitter in nature, and is a laxative, and can activate blood circulation and remove blood stasis, and is the main drug; Angelica, Chuanxiong, and peach kernel can activate blood circulation and remove blood stasis to enhance the function of Yimu grass, and can replenish blood and activate blood circulation, and Astragalus can replenish the spleen and qi and regulate qi, and are the ministers; Paojiang warms the meridians and dispels cold as an adjuvant, and Zhigancao harmonizes all the drugs, is sweet and slow to replenish the middle, and is the guiding drug, which significantly improves the original Yimu Biochemical Combination in treating a series of postpartum syndromes such as postpartum lochia retention, abdominal pain caused by blood stasis, and insufficient qi and blood in female livestock. The combination of all the drugs can play the effects of activating blood circulation and removing blood stasis, warming the meridians and relieving pain. The results of modern pharmacodynamics studies show that the stachydrine and leonurine contained in motherwort, the angelica polysaccharides and ferulic acid contained in angelica and Chuanxiong, and the amygdalin contained in peach kernel all have the functions of exciting the uterus, promoting uterine contraction, improving blood flow rheology, and regulating cardiovascular function. It has good effects on promoting postpartum uterine involution, repairing postpartum endometrium, anti-inflammatory, enhancing immunity, and antibacterial. The above pharmacological research results show that the veterinary motherwort biochemical mixture has the ability to fundamentally prevent and treat a series of postpartum syndromes such as postpartum lochia, blood stasis and abdominal pain, and insufficient qi and blood in female livestock, which fully reflects the basic concept of "treating the root cause of the disease" in traditional Chinese medicine, and also reflects the concept of "preventing disease before it occurs" in traditional Chinese medicine, while improving body shape to achieve postpartum prevention and treatment of female livestock.

[0080] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A veterinary Leonurus biochemical mixture, characterized in that: The veterinary Leonurus biochemical mixture comprises the following components by weight: 6-10 parts of Leonurus herb, 3-6 parts of Angelica sinensis, 3-6 parts of Chuanxiong, 3-6 parts of peach kernel, 3-6 parts of Astragalus, 3-6 parts of roasted ginger and 3-6 parts of roasted liquorice.

2. The veterinary Leonurus biochemical mixture according to claim 1, characterized in that: The veterinary Leonurus biochemical mixture comprises the following components by weight: 4 parts of Leonurus herb, 2 parts of Angelica sinensis, 2 parts of Chuanxiong, 2 parts of Peach Kernel, 1 part of Astragalus, 2 parts of Paojiang and 2 parts of Zhigancao.

3. The veterinary Leonurus biochemical mixture according to claim 1, characterized in that: The veterinary Leonurus biochemical mixture comprises the following components by weight: 8 parts of Leonurus herb, 4 parts of Angelica sinensis, 4 parts of Chuanxiong, 4 parts of Peach Kernel, 2 parts of Astragalus, 4 parts of Paojiang and 4 parts of Zhigancao.

4. A method for preparing a veterinary Leonurus biochemical mixture, characterized in that: The details are as follows: Step 1, weigh the raw materials required for the veterinary Leonurus biochemical mixture according to the weight parts and set aside; Step 2: Grind the astragalus into medium powder, extract with 10 times the volume of calcium oxide aqueous solution, reflux and concentrate to obtain an astragalus extract; Step 3, pulverize angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction with 5 times volume of ethanol, evaporate the ethanol, and obtain a mixed extract; Step 4, crushing the motherwort, performing Soxhlet extraction with 10 times the volume of ethanol, and evaporating the ethanol to obtain a motherwort extract; Step 5, respectively mixing the astragalus extract, the mixed extract and the motherwort extract obtained in step 2 to step 5, adding an equal amount of distilled water, stirring evenly, filtering, and obtaining a mixed medicinal solution; Step 6: sampling and testing the mixed medicinal solution obtained in step 5, and after passing the test, filling and sterilizing to obtain the veterinary Leonurus biochemical mixture.

5. The method for preparing the veterinary Leonurus biochemical mixture according to claim 4, characterized in that: The step 2 is specifically as follows: The astragalus was crushed into medium powder, soaked in 10 times the volume of 1% calcium oxide aqueous solution for 30 minutes, refluxed and extracted twice, each time for 1 hour, the filtrates were combined and concentrated to obtain the astragalus extract.

6. The method for preparing the veterinary Leonurus biochemical mixture according to claim 4, characterized in that: The step three is as follows: Grind angelica, chuanxiong, peach kernel, roasted ginger and roasted licorice into medium powder, perform flash extraction multiple times with 5 times volume of 80% ethanol, each time for 5-7 minutes, combine the multiple flash extractions, concentrate under reduced pressure to recover ethanol, and obtain a mixed extract.

7. The method for preparing the veterinary Leonurus biochemical mixture according to claim 6, characterized in that: The concentration of ethanol during Soxhlet extraction is 90%-95%.

8. The method for preparing the veterinary Leonurus biochemical mixture according to any one of claims 4 to 7, characterized in that: In step five, in every 300 ml of mixed medicinal liquid, the raw drug amount of Astragalus is 20 g, the raw drug amount of Leonurus japonicus is 80 g, the raw drug amount of Angelica sinensis is 40 g, the raw drug amount of Ligusticum chuanxiong is 40 g, the raw drug amount of Peach Kernel is 40 g, the raw drug amount of Zingiber officinale is 40 g and the raw drug amount of Radix Glycyrrhizae uralensis is 40 g.