Medical paste dressing for promoting wound healing and preparation method and application thereof

Abstract

The invention discloses a medical paste dressing for promoting wound healing and a preparation method and application thereof. The medical paste dressing is prepared from the following raw materials: carbomer, hyaluronic acid, glycerol, tridecapeptide, vaseline, Tween, a manganese element additive, a natural plant extract, L-gluconic acid-1, 5-lactone and the balance of purified water, and the healing speed of a wound surface is increased by reasonably proportioning the various components. Wherein the manganese element promotes collagen synthesis and oxidation resistance, the plant extract provides anti-inflammatory and antibacterial support, the L-gluconic acid-1, 5-lactone further accelerates the healing process by promoting extracellular matrix synthesis and keeping a moist environment, and the various beneficial components have a synergistic effect. The medical paste dressing for promoting wound healing disclosed by the invention not only has a relatively good healing promoting effect on common wounds, but also has a very remarkable healing promoting effect on wounds caused by burns, and scar hyperplasia is relieved.

Description

Technical Field

[0001] The invention relates to the technical field of medical dressings, and in particular to a medical paste dressing for promoting wound healing, and a preparation method and application thereof. Background Art

[0002] Wound healing is a process of self-repair of the body, involving multiple biological mechanisms, including inflammatory response, cell proliferation and remodeling. Effective wound healing is of great significance for maintaining body health, preventing infection, reducing complications and accelerating recovery. Poor wound healing not only prolongs treatment time, but may also lead to chronic trauma, infection or other serious complications, causing pain to patients and increasing medical costs. Therefore, the development of efficient wound healing products is crucial to improving the quality of life of patients and reducing the medical burden. With the development of science and technology, a variety of products that promote wound healing have appeared on the market. According to the characteristics and functions of the materials, these products can be roughly divided into the following categories: (1) Traditional dressings, such as gauze and cotton balls, are mainly used to protect wounds and absorb exudate. However, they lack the function of promoting healing and may adhere to the wound during use, causing secondary damage; (2) Hygroscopic dressings, including hydrocolloid dressings and foam dressings, can keep the wound moist and create a suitable healing environment; (3) Antimicrobial dressings contain silver ions, iodine or other antimicrobial ingredients, which can effectively inhibit wound infection. Although such dressings can reduce the risk of infection in the short term, long-term use may lead to drug resistance problems and may cause allergic reactions in some patients; (4) Biomaterial dressings, such as collagen dressings and alginate dressings, use biocompatible materials to promote cell proliferation and tissue regeneration; (5) Drug-releasing dressings can gradually release growth factors, antibiotics and other drugs into the wound to promote cell proliferation and tissue regeneration in a targeted manner.

[0003] Despite the wide variety of wound healing products, there are still some problems that need to be solved, mainly in the following aspects: (1) Some chemical components may irritate the wound, affect the healing effect, and even cause allergic reactions; (2) Insufficient humidity control, some dressings may not be effective in keeping the wound moist, resulting in dry or overly moist wounds, thus affecting the healing process; (3) Some synthetic materials are difficult to degrade after use, causing environmental pollution and affecting the patient's comfort; (4) The instability of drug release leads to uncertainty in its therapeutic effect. Therefore, it is urgent to develop a medical dressing that promotes wound healing, and through the regulation of the formula, it can achieve multiple ways to promote rapid wound healing and accelerate the healing speed. Summary of the invention

[0004] In view of this, the present invention proposes a medical paste dressing for promoting wound healing and a preparation method and application thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A medical paste dressing for promoting wound healing comprises the following raw material components by weight percentage: 0.1% to 1% carbomer, 0.05% to 0.25% hyaluronic acid, 1% to 5% glycerol, 0.05% to 0.1% tridecapeptide, 2% to 5% vaseline, 1% to 3% Tween, 0.05% to 0.15% manganese element additive, 0.2% to 0.6% natural plant extract, 0.02% to 0.08% L-glucono-1,5-lactone, and the balance purified water.

[0007] Preferably, the natural plant extract is a mixture of carnosic acid and epimedium polysaccharide.

[0008] Preferably, the mass ratio of carnosic acid to epimedium polysaccharide is 2 to 5:1.

[0009] Preferably, the preparation method of the epimedium polysaccharide is: extracting the dried leaves of epimedium with hot water, filtering, adding ethanol to the filtrate after concentrating the filtrate, centrifuging after standing, separating the precipitate, deproteinizing the precipitate, dialyzing and freeze-drying to obtain epimedium polysaccharide; the hot water extraction conditions include: temperature control at 80-100°C, water-to-material ratio of 20-30:1 (mL / g), and extraction time of 2-3 hours.

[0010] Preferably, the manganese element additive includes an organic manganese compound.

[0011] Preferably, the organic manganese compound includes one or more of manganese gluconate and manganese acetate.

[0012] Preferably, the Tween is one or more of Tween-80, Tween-60 and Tween-40.

[0013] The present invention also provides a method for preparing a medical paste dressing for promoting wound healing, comprising the following steps:

[0014] S1. Take 40% to 60% of the total amount of purified water, slowly add carbomer, hyaluronic acid, tridecapeptide, manganese element additive, natural plant extract, and L-glucono-1,5-lactone in proportion, stir at room temperature at a stirring speed of 80 to 100 r / min for 20 to 30 minutes, and let stand for 1 to 2 hours to obtain a premixed solution A;

[0015] S2. Take 60% to 40% of the total amount of purified water, add glycerin, vaseline and Tween in proportion, stir at room temperature at a stirring speed of 100 to 150 r / min for 20 to 30 minutes, and let it stand for 1 to 2 hours to obtain a premixed solution B;

[0016] S3. Slowly pour the premixed liquid A into the container containing the premixed liquid B, stir evenly, and perform high-pressure homogenization to obtain a medical ointment dressing that promotes wound healing.

[0017] Preferably, the high-pressure homogenization pressure is 150-200 MPa, the homogenization temperature is 50-60° C., and the homogenization speed is 1200-2000 r / min.

[0018] The present invention provides application of the above-mentioned medical paste dressing for promoting wound healing in preparing products for reducing scars and promoting wound healing.

[0019] The present invention provides the use of the medical paste dressing for promoting wound healing in the preparation of a product for promoting wound healing caused by burns.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention provides a medical paste dressing for promoting wound healing. On the basis of carbomer, hyaluronic acid, glycerol, tridecapeptide, vaseline, tween, and purified water, manganese element additives, natural plant extracts, and L-glucono-1,5-lactone are added for combined use to achieve synergistic synergy and jointly improve the wound healing speed. Manganese element promotes collagen synthesis and anti-oxidation, plant extracts provide anti-inflammatory and antibacterial support, and L-glucono-1,5-lactone further accelerates the healing process by promoting extracellular matrix synthesis and maintaining a moist environment, thereby achieving multi-pathway promotion of wound healing. The preparation process of the medical paste dressing for promoting wound healing provided by the present invention is simple. When it is prepared into solutions A and B, high-pressure homogenization and emulsification can be performed, so that each component is fully dissolved and emulsified, and the dressing is continuously moisturized, moisturized, and stable. The oily component wraps the aqueous phase active component so that the effective component is slowly released, the biological activity is stable, the duration of the drug effect is extended, and the wound healing is accelerated. DETAILED DESCRIPTION

[0022] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0023] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0024] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0025] The preparation method of the epimedium polysaccharide of the invention comprises the following steps: taking the dried leaves of the epimedium, extracting them with hot water, controlling the extraction temperature to be 90 DEG C, the water-to-material ratio to be 25:1 (mL / g), extracting for 2 hours, filtering, adding 5 times the volume of 75% ethanol to the filtrate, standing at 5 DEG C for 24 hours, centrifuging, separating the precipitate, re-dissolving the precipitate, adding 1 / 5 volume of Sevage solution (the mass ratio of chloroform to n-butanol is 5:1) to remove protein, shaking on a shaker for 30 minutes, standing and centrifuging to obtain the upper polysaccharide solution, repeating the steps for multiple times until no protein is detected, rotating and concentrating, dialyzing (with a molecular weight cutoff of 2000Da), and freeze-drying for storage to obtain the epimedium polysaccharide.

[0026] Example 1

[0027] A medical paste dressing for promoting wound healing comprises the following raw material components by weight percentage: 0.1% carbomer, 0.25% hyaluronic acid, 1% glycerol, 0.1% tridecapeptide, 2% vaseline, 1% Tween-80, 0.05% manganese acetate, 0.6% natural plant extract, 0.08% L-glucono-1,5-lactone, and the balance purified water; wherein the natural plant extract is a mixture of carnosic acid and epimedium polysaccharide in a mass ratio of 2:1.

[0028] The method for preparing the medical paste dressing for promoting wound healing comprises the following steps:

[0029] S1. Take 40% of the total amount of purified water, slowly add carbomer, hyaluronic acid, tridecapeptide, manganese acetate, natural plant extract, and L-glucono-1,5-lactone in proportion, stir well at room temperature at a stirring speed of 80 r / min for 30 min, and let stand for 1 h to obtain a premixed solution A;

[0030] S2. Take 60% of the total amount of purified water, add glycerol, vaseline and Tween in proportion, stir thoroughly at room temperature at a stirring speed of 100 r / min for 30 minutes, and let stand for 1 hour to obtain premixed solution B;

[0031] S3. Slowly pour the premixed liquid A into the container containing the premixed liquid B, stir evenly, and perform high-pressure homogenization. The high-pressure homogenization pressure is 150 MPa, the homogenization temperature is 50°C, and the homogenization speed is 2000 r / min to obtain a medical ointment dressing that promotes wound healing.

[0032] Example 2

[0033] A medical paste dressing for promoting wound healing comprises the following raw material components by weight percentage: 0.5% carbomer, 0.1% hyaluronic acid, 3% glycerol, 0.08% tridecapeptide, 4% vaseline, 2% Tween-60, 0.1% manganese gluconate, 0.4% natural plant extract, 0.04% L-glucono-1,5-lactone, and the balance purified water; wherein the natural plant extract is a mixture of carnosic acid and epimedium polysaccharide in a mass ratio of 4:1.

[0034] The method for preparing the medical paste dressing for promoting wound healing comprises the following steps:

[0035] S1. Take 50% of the total amount of purified water, slowly add carbomer, hyaluronic acid, tridecapeptide, manganese gluconate, natural plant extract, and L-glucono-1,5-lactone in proportion, stir well at room temperature at a stirring speed of 100 r / min for 30 minutes, and let stand for 2 hours to obtain a premixed solution A;

[0036] S2. Take 50% of the total amount of purified water, add glycerol, vaseline and Tween in proportion, stir thoroughly at room temperature at a stirring speed of 150 r / min for 20 min, and let stand for 2 h to obtain premix B;

[0037] S3. Slowly pour the premixed liquid A into the container containing the premixed liquid B, stir evenly, and perform high-pressure homogenization. The high-pressure homogenization pressure is 160MPa, the homogenization temperature is 60°C, and the homogenization speed is 1500r / min, so as to obtain a medical ointment dressing that promotes wound healing.

[0038] Example 3

[0039] A medical ointment dressing for promoting wound healing comprises the following raw material components by weight percentage: 1% carbomer, 0.05% hyaluronic acid, 5% glycerol, 0.05% tridecapeptide, 5% vaseline, 3% Tween-40, 0.05% manganese acetate, 0.1% manganese gluconate, 0.2% natural plant extract, 0.02% L-glucono-1,5-lactone, and the balance purified water; wherein the natural plant extract is a mixture of carnosic acid and epimedium polysaccharide in a mass ratio of 5:1.

[0040] The method for preparing the medical paste dressing for promoting wound healing comprises the following steps:

[0041] S1. Take 60% of the total amount of purified water, slowly add carbomer, hyaluronic acid, tridecapeptide, manganese gluconate, natural plant extract, and L-glucono-1,5-lactone in proportion, stir well at room temperature at a stirring speed of 100 r / min for 20 minutes, and let stand for 1 hour to obtain a premixed solution A;

[0042] S2. Take 40% of the total amount of purified water, add glycerol, vaseline and Tween in proportion, stir at room temperature at a stirring speed of 100 r / min for 30 minutes, and let it stand for 1 hour to obtain premixed solution B;

[0043] S3. Slowly pour the premixed liquid A into the container containing the premixed liquid B, stir evenly, and perform high-pressure homogenization. The high-pressure homogenization pressure is 200 MPa, the homogenization temperature is 50°C, and the homogenization speed is 1200 r / min to obtain a medical ointment dressing that promotes wound healing.

[0044] Comparative Example 1

[0045] The difference between this comparative example and Example 2 is that the medical paste dressing for promoting wound healing in this example does not contain manganese element additives; the preparation method of the paste dressing in this example is basically the same as that of Example 2.

[0046] Comparative Example 2

[0047] The difference between this comparative example and Example 2 is that the medical paste dressing for promoting wound healing in this example does not contain L-glucono-1,5-lactone; the preparation method of the paste dressing in this example is basically the same as that in Example 2.

[0048] Comparative Example 3

[0049] The difference between this comparative example and Example 2 is that the natural plant extract in the medical paste dressing for promoting wound healing in this example is a single carnosic acid; the preparation method of the paste dressing in this example is basically the same as that in Example 2.

[0050] Comparative Example 4

[0051] The difference between this comparative example and Example 2 is that the natural plant extract in the medical paste dressing for promoting wound healing in this example is a single epimedium polysaccharide; the preparation method of the paste dressing in this example is basically the same as that in Example 2.

[0052] Comparative Example 5

[0053] The difference between this comparative example and Example 2 is that the natural plant extract in the medical paste dressing for promoting wound healing in this example is a mixture of carnosic acid and epimedium polysaccharide in a mass ratio of 1:4; the preparation method of the paste dressing in this example is basically the same as that in Example 2.

[0054] Comparative Example 6

[0055] The difference between this comparative example and Example 2 is that the manganese gluconate in the medical paste dressing for promoting wound healing in this example is replaced by manganese chloride; the preparation method of the paste dressing in this example is basically the same as that in Example 2.

[0056] Test Example 1 Safety Experiment

[0057] The medical ointment dressings for promoting wound healing of Examples 1-3 and Comparative Examples 1-6 were subjected to animal skin irritation test, skin sensitization test and in vitro cytotoxicity test, respectively, and the results showed that they were safe and non-toxic.

[0058] Test Example 2 Antibacterial Experiment

[0059] The antibacterial efficacy was determined according to the "Antibacterial Efficacy Test Method" of the 2020 edition of the Chinese Pharmacopoeia, and the number of colonies surviving 24 hours in the test solution Sabouraud grape liquid culture medium was determined. The test bacteria were: Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, Propionibacterium acnes. The antibacterial rates of the medical ointment dressings prepared in Examples 1 to 3 and Comparative Examples 1 to 6 are shown in Table 1 below.

[0060] Table 1 Antibacterial effect of medical paste dressings

[0061]

[0062] It can be seen from the above table that the medical ointment dressing for promoting wound healing prepared by the present invention can have an inhibition rate of greater than 99.9% on Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, and Propionibacterium acnes; the results of Comparative Examples 1 and 6 show that organic manganese compounds have better biocompatibility and antibacterial activity than inorganic manganese compounds; Comparative Example 2 does not add L-glucono-1,5-lactone, and its antibacterial effect is significantly weakened; The results of Comparative Examples 3 to 5 show that the introduction of natural plant extracts carnosic acid and epimedium polysaccharide has a good synergistic effect under a reasonable ratio.

[0063] Test Example 3 General Wound Repair Performance

[0064] 100 SPF SD rats were randomly divided into 10 groups, each with 10 rats, and the wound healing-promoting ointment dressings prepared in Examples 1-3 and Comparative Examples 1-6 and the commercially available medical care ointment dressing (Lan Ke Fu Ning) were applied respectively. Each rat was anesthetized by intraperitoneal injection of 1.5 ml / kg 10% chloral hydrate, and then the dorsal hair was removed and the skin was disinfected. A 1.5 cm2 wound was cut off from the back of each rat. 2 A wound of full thickness. Then, the medical paste dressings of Examples 1-3, Comparative Examples 1-6 and commercially available medical care paste dressings were applied to the wound surface, once in the morning and once in the evening every day. The healing of the wounds of the rats and the scars after complete healing were observed on the 2nd, 4th and 6th days after application. Wound healing rate = (initial wound area - wound area measured on the nth day) / initial wound area × 100%; after complete healing, the wound surface is closed, no scars are seen, and the skin surface is smooth, which is judged to be well repaired, and the proportion of well-repaired rats is counted; the experimental results are shown in Table 2.

[0065] Table 2 General wound repair performance of medical paste dressings

[0066]

[0067] The above results show that the medical ointment dressings for promoting wound healing prepared in Examples 1-3 of the present invention have a good repair effect on skin damage, fast skin healing speed and high repair efficiency. Comparative Example 1 does not add organic manganese compounds, and its wound healing rate is significantly slowed down. Comparative Example 6 adds inorganic manganese compounds and the repair effect is weakened to a certain extent, indicating that organic manganese compounds can significantly promote skin wound repair, have excellent biocompatibility, biological activity and the ability to promote new tissue formation, stimulate cell proliferation and migration, and thus accelerate wound healing. Comparative Example 2 does not add L-glucono-1,5-lactone, and its wound healing rate is significantly slowed down; the results of Comparative Examples 3 to 5 show that the addition of a certain ratio of natural plant extracts carnosic acid and epimedium polysaccharide can synergistically significantly improve the repair efficiency, shorten the healing time, and increase the proportion of good repairs.

[0068] Test Example 4 Burn Wound Repair Performance

[0069] Take 110 SPF SD rats; randomly divide them into 11 groups, 10 rats in each group, and apply the medical paste dressings for promoting wound healing prepared in Examples 1-3 and Comparative Examples 1-6 and the commercially available medical burn dressing (Cuineng) respectively. Each rat is anesthetized by intraperitoneal injection of 1.5ml / kg 10% chloral hydrate, then the back hair is removed and the skin is disinfected. One day after depilation, a burn model is created on the depilated back of the rat using a scald instrument (95°C for 10 seconds). After the wound is cooled naturally for 1 hour, the products of Examples 1-3, Comparative Examples 1-6 and the control group are applied respectively, and physiological saline is used as a blank group. Apply once in the morning and evening every day, and observe the healing of the wound surface of the rat in different time periods. The relevant results are shown in Table 3.

[0070] Table 3 Burn wound repair performance of medical paste dressings

[0071]

[0072] The above results show that the medical ointment dressings for promoting wound healing prepared in Examples 1-3 of the present invention have a good repairing effect on skin damage caused by burns.

[0073] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A medical paste dressing for promoting wound healing, characterized in that: The invention comprises the following raw material components by weight percentage: 0.1% to 1% of carbomer, 0.05% to 0.25% of hyaluronic acid, 1% to 5% of glycerol, 0.05% to 0.1% of tridecapeptide, 2% to 5% of vaseline, 1% to 3% of Tween, 0.05% to 0.15% of manganese element additive, 0.2% to 0.6% of natural plant extract, 0.02% to 0.08% of L-glucono-1,5-lactone, and the balance of purified water.

2. A medical paste dressing for promoting wound healing according to claim 1, characterized in that: The natural plant extract is a mixture of carnosic acid and epimedium polysaccharide.

3. A medical paste dressing for promoting wound healing according to claim 2, characterized in that: The mass ratio of carnosic acid to epimedium polysaccharide is 2-5:

1.

4. The medical paste dressing for promoting wound healing according to claim 2, characterized in that: The preparation method of the epimedium polysaccharide comprises the following steps: extracting the dried leaves of epimedium with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, centrifuging after standing, separating the precipitate, deproteinizing the precipitate, dialyzing and freeze-drying to obtain the epimedium polysaccharide; the hot water extraction conditions include: controlling the temperature to 80-100°C, the water-to-material ratio to 20-30:1 (mL / g), and the extraction time to 2-3 hours.

5. The medical paste dressing for promoting wound healing according to claim 1, characterized in that: The manganese element additive includes an organic manganese compound.

6. The medical paste dressing for promoting wound healing according to claim 5, characterized in that: The organic manganese compound includes one or more of manganese gluconate and manganese acetate.

7. A method for preparing a medical paste dressing for promoting wound healing according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Take 40% to 60% of the total amount of purified water, slowly add carbomer, hyaluronic acid, tridecapeptide, manganese element additive, natural plant extract, and L-glucono-1,5-lactone in proportion, stir at room temperature at a stirring speed of 80 to 100 r / min for 20 to 30 minutes, and let stand for 1 to 2 hours to obtain a premixed solution A; S2. Take 60% to 40% of the total amount of purified water, add glycerin, vaseline and Tween in proportion, stir at room temperature at a stirring speed of 100 to 150 r / min for 20 to 30 minutes, and let it stand for 1 to 2 hours to obtain a premixed solution B; S3. Slowly pour the premixed liquid A into the container containing the premixed liquid B, stir evenly, and perform high-pressure homogenization to obtain a medical ointment dressing that promotes wound healing.

8. The method for preparing a medical paste dressing for promoting wound healing according to claim 7, characterized in that: The high-pressure homogenization pressure is 150-200 MPa, the homogenization temperature is 50-60° C., and the homogenization speed is 1200-2000 r / min.

9. Use of the medical ointment dressing for promoting wound healing according to any one of claims 1 to 6 in the preparation of products for reducing scars and promoting wound healing.

10. Use of the medical paste dressing for promoting wound healing according to any one of claims 1 to 6 in the preparation of a product for promoting wound healing caused by burns.

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