Low-energy-consumption green preparation process of alanine fermentation liquor fertilizer

Abstract

The invention discloses a low-energy-consumption green preparation process of an alanine fermentation broth fertilizer, and relates to the technical field of bio-fertilizer preparation, and the low-energy-consumption green preparation process comprises the operation flow of pretreatment, blending, concentration and mixing of an alanine fermentation broth. The method has the advantages that the filling material and the lignocellulose nanoparticles are doped into the concentrated alanine fermentation liquor, so that the stability and slow release performance of the fertilizer can be improved, meanwhile, loss and change of nutritional ingredients in the storage and use process are reduced, nitrogen, phosphorus, potassium and trace elements are added into the alanine fermentation liquor, and the yield of the fertilizer is increased. Nutrient requirements of different crops in different growth stages are met, the pertinence of the alanine fermentation liquor fertilizer is improved, it is ensured that nutritional ingredients are evenly distributed in fermentation liquor, the biological activity of the fertilizer is enhanced by introducing a plant growth regulator and a beneficial microbial agent, and through low-temperature standing, a siphoning method and solar evaporation concentration, the yield of the fertilizer is increased. The influence of high energy consumption of traditional mechanical filtration is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of biological fertilizer preparation, in particular to a green preparation process of alanine fermentation liquid fertilizer with low energy consumption. Background Art

[0002] With the increasing attention paid to environmental protection and sustainable development around the world, the demand for green and efficient fertilizers in the agricultural field is growing. Traditional fertilizer preparation processes often have problems such as high energy consumption, waste of resources, and great pressure on the environment. Under such circumstances, it is imperative to develop low-energy fertilizer preparation processes. Alanine is an important amino acid, and its fermentation broth has great potential in the fertilizer field. However, the existing alanine fermentation broth fertilizer preparation process has many shortcomings. On the one hand, the existing process has high energy consumption. In the pretreatment of the fermentation broth, mechanical filtration and other methods are often used. These methods not only consume a lot of energy, but may also destroy the effective ingredients in the fermentation broth. In the concentration stage, it mostly relies on traditional evaporation and concentration methods, which consume a lot of heat energy and increase production costs. In the fertilizer molding process, some processes may require high temperature and high pressure conditions, which further aggravates energy consumption. On the other hand, traditional processes may introduce some chemicals or produce by-products during the preparation process, which may cause potential harm to the soil and ecological environment. For example, some additives may remain in the soil, affecting the ecological balance of the soil. If the wastewater and waste gas generated by some processes are not properly treated, it will cause pollution to the environment.

[0003] In addition, the existing process needs to be improved in terms of the formulation and stability of nutrients. It is often unable to reasonably add nutrients according to the precise needs of different crops at different growth stages, resulting in the fertilizer being not very targeted. At the same time, nutrients are easily lost or changed during storage and use, affecting the effect and durability of the fertilizer. For this reason, we propose a low-energy green preparation process for alanine fermentation liquid fertilizer. Summary of the invention

[0004] The purpose of the present invention is to provide a low-energy-consumption green preparation process of alanine fermentation liquid fertilizer.

[0005] In order to solve the problems raised in the above background technology, the present invention provides the following technical solutions: a low-energy consumption alanine fermentation broth fertilizer green preparation process, including the operation process of pretreatment, preparation, concentration and mixing of alanine fermentation broth, and the specific operation steps of the low-energy consumption alanine fermentation broth fertilizer green preparation process are as follows:

[0006] Step 1, pre-treating the crude alanine fermentation broth, transferring the crude alanine fermentation broth into a low-temperature static tank for static, taking the supernatant after impurities are precipitated, ultrasonically treating the supernatant of the alanine fermentation broth to obtain the alanine fermentation broth, and transferring the supernatant into a container for standby use;

[0007] Step 2: slowly add nitrogen, phosphorus, potassium and trace elements to the container containing the alanine fermentation liquid, stir until the substances are mixed in the alanine fermentation liquid, and place the alanine fermentation liquid container in a well-ventilated area for natural fermentation;

[0008] Step 3: Build a solar evaporation device to use the heat of solar energy to slowly evaporate and concentrate the fermentation liquid, and continuously stir the alanine fermentation liquid during the evaporation process;

[0009] Step 4: Put the concentrated alanine fermentation liquid into a stirring tank, add the filling material and the lignocellulose nanoparticles, and continue stirring to mix. After the stirring is completed, a crude alanine fermentation liquid fertilizer is obtained, and the crude alanine fermentation liquid fertilizer is pressed into a regular shape as required;

[0010] Step 5: Use chemical and physical analysis methods to test the quality of the crude alanine fermentation broth fertilizer and determine the nutrient content therein.

[0011] As a further scheme of the present invention: in the step 1, the alanine fermentation broth is transferred to a static tank at 4°C-6°C, and each 1000L of alanine fermentation broth needs to be left to stand for 24h-36h. After the standing is completed, the supernatant in the static tank is taken out by siphoning and transferred to a corrosion-resistant container for standby use. The flow rate should be controlled at 5L / min-10L / min during the siphoning process. After the supernatant is transferred, an ultrasonic treatment step is introduced to treat the alanine fermentation broth for 10min-20min. After the ultrasonic treatment is completed.

[0012] As a further scheme of the present invention: in the step 2, the masses of nitrogen, phosphorus, potassium and trace elements are adjusted according to the nutritional requirements of different crops and the volume of the fermentation liquid. The masses of nitrogen, phosphorus, potassium and trace elements added to every 1000L of fermentation liquid are 10kg-15kg, 7kg-10kg, 8kg-12kg and 1kg-2kg, respectively. The trace elements include iron, zinc and copper, and the masses of the three trace elements are 0.5kg-1kg, 0.3kg-0.6kg and 0.2kg-0.4kg, respectively.

[0013] As a further solution of the present invention: in the step 2, the calculated nutrient elements are added to the container containing the alanine fermentation liquid by slow manual addition, and a wooden stick is used for continuous stirring during the addition process, the stirring rate is 30r / min-50r / min, and the stirring time is 10min-15min. Plant growth regulators and beneficial microbial agents are introduced during stirring, and the amount of added biologically active substances is controlled.

[0014] As a further solution of the present invention: in the step three, a simple solar evaporation device is built, which is mainly composed of a shallow-bottomed black metal container, a transparent glass cover and a heat-insulating material. The alanine fermentation liquid is poured into the solar evaporation device. Under sufficient sunlight, every 1000L of alanine fermentation liquid needs to be evaporated and concentrated for 3d-5d. During the evaporation process, the alanine fermentation liquid is stirred at a stirring rate of 50r / min-80r / min, stirred once every 1h-2h, and stirred for 5min-10min each time.

[0015] As a further scheme of the present invention: in the step 4, the concentrated alanine fermentation broth is loaded into a stirring tank, and a filler material is added according to 30%-50% of the volume of the alanine fermentation broth and 5%-10% of the volume of the alanine fermentation broth. The lignocellulose nanoparticles are mixed by combining low-speed mechanical stirring and natural wind-assisted stirring, the mechanical stirring speed is controlled between 80r / min-120r / min, and the stirring time is 1h-2h.

[0016] As a further solution of the present invention: in the step four, the filling material is selected from natural degradable plant fiber powder, specifically one of rice husk powder and wheat bran powder, the particle size of the added filling material is controlled between 20-50 mesh, and the selected wood cellulose nanoparticles have a diameter between 40nm-80nm and a length between 200nm-400nm.

[0017] As a further solution of the present invention: in the step 4, the crude alanine fermentation liquid fertilizer after stirring is pressed into a spherical shape with a diameter of 2cm-3cm.

[0018] As a further solution of the present invention: in the step five, the quality of the crude alanine fermentation broth fertilizer is monitored using chemical and physical analysis methods. The chemical analysis method includes using pH test paper to determine the pH value of the fertilizer and controlling it between 6.5 and 7.5. At the same time, a chemical titration method is used to determine the nutrient content and monitor the nitrogen, phosphorus, potassium and trace element contents to ensure that they meet the expected standards. The physical analysis method includes determining the parameters of the particle size, density and porosity of the fertilizer.

[0019] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are:

[0020] 1. The present invention can improve the stability and slow-release performance of the fertilizer by adding filler materials and lignocellulose nanoparticles into the concentrated alanine fermentation broth, and reduce the loss and change of nutrients during storage and use. By adding nitrogen, phosphorus, potassium and trace elements to the alanine fermentation broth, the nutritional requirements of different crops at different growth stages are met, the pertinence of the alanine fermentation broth fertilizer is improved, and the nutrients are ensured to be evenly distributed in the fermentation broth. By introducing plant growth regulators and beneficial microbial agents, the biological activity of the fertilizer is enhanced. By low-temperature standing, siphon method and solar evaporation concentration, the influence of high energy consumption of traditional mechanical filtration is avoided, and the damage to the effective ingredients of the fermentation broth and the production cost of the preparation process are reduced, the dependence on traditional energy sources is reduced, and carbon emissions are reduced;

[0021] 2. The present invention conducts comprehensive quality monitoring on the crude alanine fermentation broth fertilizer by chemical and physical analysis methods to ensure that the fertilizer is in an appropriate pH range, which is beneficial to the absorption and growth of crops. The nutrient content is determined by chemical titration, and the contents of nitrogen, phosphorus, potassium and trace elements are strictly monitored to meet the expected standards, thereby ensuring the nutritional value of the alanine fermentation broth fertilizer. The physical analysis method is used to determine the parameters such as the particle size, density and porosity of the fertilizer, which is helpful to understand the physical properties of the fertilizer and provide a basis for further optimizing the preparation process of the fertilizer. In the mixing and molding stages, the filling materials are selected from natural degradable plant fiber powder and wood cellulose nanoparticles. The added materials can be naturally degraded in the soil and will not cause long-term pollution to the soil and the environment. The entire preparation process focuses on the recycling of resources, reduces the negative impact on the environment, and meets the current global requirements for environmental protection and sustainable development. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the process flow of preparing alanine fermentation liquid fertilizer in an embodiment of the present invention;

[0023] Figure 2 Schematic diagram for comparing the fertilizer properties of alanine fermentation broth in an embodiment of the present invention. DETAILED DESCRIPTION

[0024] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0025] The present invention discloses a low-energy consumption green preparation process of alanine fermentation liquid fertilizer, which includes the operation process of pretreatment, preparation, concentration and mixing of alanine fermentation liquid. The specific operation steps of the low-energy consumption green preparation process of alanine fermentation liquid fertilizer are as follows:

[0026] Step 1, pre-treating the crude alanine fermentation broth, transferring the crude alanine fermentation broth into a low-temperature static tank for static, taking the supernatant after impurities are precipitated, ultrasonically treating the supernatant of the alanine fermentation broth to obtain the alanine fermentation broth, and transferring the supernatant into a container for standby use;

[0027] Step 2: slowly add nitrogen, phosphorus, potassium and trace elements to the container containing the alanine fermentation liquid, stir until the substances are mixed in the alanine fermentation liquid, and place the alanine fermentation liquid container in a well-ventilated area for natural fermentation;

[0028] Step 3: Build a solar evaporation device, use the heat of solar energy to slowly evaporate and concentrate the fermentation liquid, and continuously stir the alanine fermentation liquid during the evaporation process;

[0029] Step 4: Put the concentrated alanine fermentation liquid into a stirring tank, add the filling material and the lignocellulose nanoparticles, and continue stirring to mix. After the stirring is completed, a crude alanine fermentation liquid fertilizer is obtained, and the crude alanine fermentation liquid fertilizer is pressed into a regular shape as required;

[0030] Step 5: Use chemical and physical analysis methods to test the quality of the crude alanine fermentation broth fertilizer and determine the nutrient content therein.

[0031] In one embodiment of the present invention: in step 1, the alanine fermentation broth is transferred to a static tank at 4°C-6°C, and each 1000L of alanine fermentation broth needs to be left to stand for 24h-36h. After the standing is completed, the supernatant in the static tank is taken out by siphoning and transferred to a corrosion-resistant container for standby use. The flow rate should be controlled at 5L / min-10L / min during the siphoning process. After the supernatant is transferred, an ultrasonic treatment step is introduced to treat the alanine fermentation broth for 10min-20min. After the ultrasonic treatment is completed.

[0032] In one embodiment of the present invention: in step 2, the masses of nitrogen, phosphorus, potassium and trace elements are adjusted according to the nutritional requirements of different crops and the volume of fermentation liquid. The masses of nitrogen, phosphorus, potassium and trace elements added to every 1000L of fermentation liquid are 10kg-15kg, 7kg-10kg, 8kg-12kg and 1kg-2kg, respectively. The trace elements include iron, zinc and copper, and the masses of the three trace elements are 0.5kg-1kg, 0.3kg-0.6kg and 0.2kg-0.4kg, respectively.

[0033] In one embodiment of the present invention: in step 2, the calculated nutrient elements are added to the container containing the alanine fermentation liquid by slow manual addition, and a wooden stick is used for continuous stirring during the addition process, the stirring rate is 30r / min-50r / min, and the stirring time is 10min-15min. During the stirring, plant growth regulators and beneficial microbial agents are introduced, and the amount of bioactive substances added is controlled.

[0034] In one embodiment of the present invention: in step three, a simple solar evaporation device is built, which is mainly composed of a shallow-bottomed black metal container, a transparent glass cover and a heat-insulating material. The alanine fermentation liquid is poured into the solar evaporation device. Under sufficient sunlight, every 1000L of alanine fermentation liquid needs to be evaporated and concentrated for 3d-5d. During the evaporation process, the alanine fermentation liquid is stirred at a stirring rate of 50r / min-80r / min, stirred every 1h-2h, and stirred for 5min-10min each time.

[0035] In one embodiment of the present invention: in step 4, the concentrated alanine fermentation broth is charged into a stirring tank, and a filler material is added according to 30%-50% of the volume of the alanine fermentation broth and 5%-10% of the volume of the alanine fermentation broth. The lignocellulose nanoparticles are mixed by a combination of low-speed mechanical stirring and natural wind-assisted stirring. The mechanical stirring speed is controlled between 80r / min-120r / min, and the stirring time is 1h-2h.

[0036] In one embodiment of the present invention: in step four, the filling material is selected from natural degradable plant fiber powder, specifically one of rice husk powder and wheat bran powder, the particle size of the added filling material is controlled between 20-50 mesh, and the selected wood cellulose nanoparticles have a diameter between 40nm-80nm and a length between 200nm-400nm.

[0037] In one embodiment of the present invention: in step 4, the crude alanine fermentation broth fertilizer after stirring is pressed into a spherical shape with a diameter of 2 cm-3 cm.

[0038] In one embodiment of the present invention: in step five, the quality of the crude alanine fermentation broth fertilizer is monitored using chemical and physical analysis methods. The chemical analysis method includes using pH test paper to determine the pH value of the fertilizer and controlling it between 6.5 and 7.5. At the same time, the chemical titration method is used to determine the nutrient content and monitor the nitrogen, phosphorus, potassium and trace element contents to meet the expected standards. The physical analysis method includes determining the parameters of the particle size, density and porosity of the fertilizer.

[0039] In one embodiment of the present invention: the ferrous metal container can absorb more solar energy heat, the transparent glass cover plays a role in heat preservation and preventing water from evaporating too quickly, and the thermal insulation material wrapped around the container can further improve the utilization efficiency of solar energy. During the evaporation process, a condensation recovery device can be set to condense and recover the water vapor generated by evaporation. On the one hand, it can reduce the loss of water, and on the other hand, it can collect pure water for other production links.

[0040] In one embodiment of the present invention, the crude alanine fermentation liquid fertilizer is pressed into a cylindrical shape with a length of 3cm-5cm and a diameter of 1cm-3cm. The pressure is adjusted according to the hardness requirement of the fertilizer. The density of the pressed alanine fermentation liquid fertilizer is 5kg / cm 2 -10kg / cm 2 .

[0041] In one embodiment of the present invention, nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria are added to the concentrated alanine fermentation broth, and the concentrated alanine fermentation broth is placed in a sterile stirring tank at a rate of 1×10 6 -1×10 8 Slowly add the microbial agent into the sterile stirring tank, continue stirring for 30min-60min, fully mix the microbial agent and the fermentation liquid, and replace the preparation of the alanine fermentation liquid with the bio-augmentation step.

[0042] In one embodiment of the present invention: the chemical excitation method is to add humic acid to the alanine fermentation broth and stir it evenly, the addition amount is 0.1g / L-1g / L, and the physical excitation method is to use ultrasonic equipment to treat the fermentation broth, the ultrasonic power is 100W-500W, the treatment time is 10min-30min, and the alanine fermentation broth is used to stimulate activity.

[0043] Embodiment 1

[0044] (1) Pretreatment of crude alanine fermentation broth:

[0045] 3500L of crude alanine fermentation broth was transferred into a low-temperature static tank at 5°C and allowed to stand for 32h;

[0046] The supernatant was removed by siphoning at a flow rate of 7 L / min and transferred into a corrosion-resistant container;

[0047] The ultrasonic treatment was performed for 16 min at a frequency of 32 kHz.

[0048] (2) Nutrient addition and natural fermentation,

[0049] For vegetable crops, 11 kg of nitrogen (in terms of ammonium nitrate), 8 kg of phosphorus (in terms of potassium dihydrogen phosphate), 9 kg of potassium (in terms of potassium sulfate) and trace elements (0.7 kg of iron, 0.4 kg of zinc and 0.25 kg of copper) were added to every 1000 L of fermentation liquid. According to the mass of 3500 L of fermentation liquid, 38.5 kg of nitrogen, 28 kg of phosphorus, 31.5 kg of potassium, 2.45 kg of iron, 1.4 kg of zinc and 0.875 kg of copper were added accordingly. The nutrients were added slowly by hand and stirred at a rate of 42 r / min for 12 min with a wooden stick. 0.6 kg of indolebutyric acid, a plant growth regulator, and 6 × 10 Bacillus amyloliquefaciens, a beneficial microbial agent, were added to every liter of fermentation liquid. 6 Colony unit, natural fermentation 38h.

[0050] (3) Solar evaporation concentration,

[0051] A solar evaporation device was built, consisting of a black iron basin, a transparent glass cover and thermal insulation rock wool. The fermentation liquid was poured into the device and evaporated and concentrated for 4 days under sufficient sunlight. The stirring rate was 70 r / min, and the mixture was stirred every 1.6 hours for 8 minutes each time. A condensation recovery device was set up to recover water for irrigation of greenery in the plant area.

[0052] (4) Mixing, molding,

[0053] Add rice husk powder accounting for 40% of the total volume of the alanine fermentation liquid to the stirring tank, and the particle size of the rice husk powder is controlled at 32 meshes. Then add cellulose nanoparticles accounting for 8% of the total volume of the alanine fermentation liquid. The diameter of the cellulose nanoparticles is 65nm and the length is 310nm. Stir at a mechanical stirring speed of 95r / min combined with natural wind assisted stirring for 1.5h, and press the crude fertilizer into a sphere with a diameter of 2.4cm.

[0054] (5) Quality monitoring,

[0055] Chemical analysis: pH 7.1;

[0056] The nitrogen content of alanine fermentation liquid fertilizer is 3.7%, the phosphorus content is 2.5%, the potassium content is 2.8%, the iron content is 0.19%, the zinc content is 0.12%, and the copper content is 0.09%.

[0057] Physical analysis: The fertilizer particle size is mainly concentrated in 1.2-3.2mm, and the density is 1.25g / cm 3 , porosity 46%

[0058] Embodiment 2

[0059] (1) Pretreatment of crude alanine fermentation broth,

[0060] 4200L of crude alanine fermentation broth was transferred to a low-temperature static tank at 4.5°C and allowed to stand for 34 hours;

[0061] The supernatant was removed by siphoning at a flow rate of 8.5 L / min and transferred into a corrosion-resistant container;

[0062] The ultrasonic treatment was performed for 18 min at a frequency of 34 kHz.

[0063] (2) Nutrient addition and natural fermentation,

[0064] For fruit crops, 13 kg of nitrogen (in terms of ammonium nitrate), 9 kg of phosphorus (in terms of potassium dihydrogen phosphate), 11 kg of potassium (in terms of potassium sulfate) and trace elements (0.8 kg of iron, 0.5 kg of zinc and 0.3 kg of copper) were added to every 1000 L of fermentation liquid. For 4200 L of fermentation liquid, 54.6 kg of nitrogen, 37.8 kg of phosphorus, 46.2 kg of potassium, 3.36 kg of iron, 2.1 kg of zinc and 1.26 kg of copper were added accordingly. Nutrient elements were added slowly by hand and stirred at a rate of 46 r / min for 13 min with a wooden stick. 0.8 kg of salicylic acid, a plant growth regulator, and 7 × 10 Bacillus polymyxa were added per liter of fermentation liquid. 6 Colony unit, natural fermentation 42h.

[0065] (3) Solar evaporation concentration,

[0066] A solar evaporation device was built, consisting of a black aluminum basin, a transparent glass cover and an insulating sponge. The fermentation liquid was poured into the device and evaporated and concentrated for 4 days under sufficient sunlight. The stirring rate was 75 r / min, and the mixture was stirred every 1.7 hours for 9 minutes each time. A condensation recovery device was set up to recover water for flushing the equipment.

[0067] (4) Mixing, molding,

[0068] Wheat bran powder (particle size controlled at 38 mesh) accounting for 45% of the volume of the fermentation liquid and wood cellulose nanoparticles (diameter 72nm, length 340nm) accounting for 9% of the volume of the fermentation liquid were added to the stirring tank. The mechanical stirring speed was 105r / min combined with natural wind assisted stirring for 1.7h, and the crude fertilizer was pressed into a sphere with a diameter of 2.6cm.

[0069] (5) Quality monitoring,

[0070] Chemical analysis: pH 7.0;

[0071] The nitrogen content of alanine fermentation liquid fertilizer is 4.4%, the phosphorus content is 3.2%, the potassium content is 3.6%, the iron content is 0.23%, the zinc content is 0.15%, and the copper content is 0.11%;

[0072] Physical analysis: The fertilizer particle size is mainly concentrated in 1.4-3.6mm, and the density is 1.3g / cm 3 , porosity 48%

[0073] Embodiment 3

[0074] (1) Pretreatment of crude alanine fermentation broth,

[0075] 3800L of crude alanine fermentation broth was transferred into a low-temperature static tank at 5.5°C and allowed to stand for 30 hours. The supernatant was taken out by siphoning at a flow rate of 7.5L / min and transferred into a corrosion-resistant container for ultrasonic treatment for 15 minutes at an ultrasonic frequency of 33kHz.

[0076] (2) Nutrient addition and natural fermentation,

[0077] For flower crops, 12 kg of nitrogen (in terms of ammonium nitrate), 8.5 kg of phosphorus (in terms of potassium dihydrogen phosphate), 10 kg of potassium (in terms of potassium sulfate) and trace elements (0.75 kg of iron, 0.45 kg of zinc and 0.28 kg of copper) were added to every 1000 L of fermentation liquid. For 3800 L of fermentation liquid, 45.6 kg of nitrogen, 32.3 kg of phosphorus, 38 kg of potassium, 2.85 kg of iron, 1.71 kg of zinc and 1.064 kg of copper were added accordingly. Nutrient elements were added slowly by hand and stirred at a rate of 44 r / min for 12.5 min with a wooden stick. 0.7 kg of jasmonic acid, a plant growth regulator, and 6.5 × 10- ... 6 Colony unit, natural fermentation 39h.

[0078] (3) Solar evaporation concentration,

[0079] A solar evaporation device was built, consisting of a black stainless steel basin, a transparent glass cover and thermal insulation foam. The fermentation liquid was poured into the device and evaporated and concentrated for 4.2 days under sufficient sunlight. The stirring rate was 72 r / min, and the mixture was stirred every 1.5 hours for 8.5 minutes each time. A condensation recovery device was set up to recover water for container cleaning.

[0080] (4) Mixing, molding,

[0081] Rice husk powder accounting for 42% of the volume of the fermentation liquid, the particle size of the rice husk powder was controlled at 34 mesh, and wood cellulose nanoparticles accounting for 8.5% of the volume of the fermentation liquid, the diameter of the wood cellulose nanoparticles was 68nm, and the length was 320nm. The crude fertilizer was pressed into a sphere with a diameter of 2.5cm at a mechanical stirring speed of 100r / min combined with natural wind-assisted stirring for 1.6h.

[0082] (5) Quality monitoring:

[0083] Chemical analysis: pH 7.2;

[0084] The nitrogen content of alanine fermentation liquid fertilizer is 4.2%, the phosphorus content is 3.1%, the potassium content is 3.5%, the iron content is 0.21%, the zinc content is 0.14%, and the copper content is 0.1%.

[0085] Physical analysis: The fertilizer particle size is mainly concentrated in 1.3-3.4mm, and the density is 1.28g / cm 3 , porosity 47%

[0086] Embodiment 4:

[0087] (1) Pretreatment of crude alanine fermentation broth,

[0088] 4500L of crude alanine fermentation broth was transferred to a low-temperature static tank at 4.8°C and allowed to stand for 35 hours. The supernatant was removed by siphoning at a flow rate of 9L / min, transferred to a corrosion-resistant container, and subjected to ultrasonic treatment for 17 minutes at a frequency of 35kHz.

[0089] (2) Nutrient addition and natural fermentation,

[0090] For economic crops, 14 kg of nitrogen (in terms of ammonium nitrate), 9.5 kg of phosphorus (in terms of potassium dihydrogen phosphate), 12 kg of potassium (in terms of potassium sulfate) and trace elements (0.85 kg of iron, 0.55 kg of zinc and 0.35 kg of copper) were added to every 1000 L of fermentation liquid. For 4500 L of fermentation liquid, 63 kg of nitrogen, 42.75 kg of phosphorus, 54 kg of potassium, 3.825 kg of iron, 2.475 kg of zinc and 1.575 kg of copper were added accordingly. Nutrient elements were added slowly by hand and stirred at a rate of 48 r / min for 14 min with a wooden stick. 0.9 kg of plant growth regulator ethephon and 8 × 10 mixed beneficial microbial agents of Bacillus were added per liter of fermentation liquid. 6 Colony unit, natural fermentation 44h.

[0091] (3) Solar evaporation concentration,

[0092] A solar evaporation device was built, consisting of a black galvanized basin, a transparent glass cover and thermal insulation rubber and plastic. The fermentation liquid was poured into the device and evaporated and concentrated for 4.8 days under sufficient sunlight. The stirring rate was 78 r / min, and the mixture was stirred every 1.8 hours for 9.5 minutes each time. A condensation recovery device was set up to recover water for irrigation of surrounding farmland.

[0093] (4) Mixing, molding,

[0094] Wheat bran powder accounting for 48% of the volume of the fermentation liquid, the particle size of the wheat bran powder was controlled at 42 mesh, and wood cellulose nanoparticles accounting for 9.5% of the volume of the fermentation liquid were added to the stirring tank. The diameter of the wood cellulose nanoparticles was 78nm and the length was 360nm. The mechanical stirring speed was 110r / min combined with natural wind assisted stirring for 1.8h, and the crude fertilizer was pressed into a sphere with a diameter of 2.7cm.

[0095] (5) Quality monitoring,

[0096] Chemical analysis: pH 7.3;

[0097] The nitrogen content is 4.8%, the phosphorus content is 3.6%, the potassium content is 4.0%, the iron content is 0.25%, the zinc content is 0.17%, and the copper content is 0.12%;

[0098] Physical analysis: The fertilizer particle size is mainly concentrated in 1.5-3.8mm, and the density is 1.32g / cm 3 , the porosity is 49%.

[0099] Embodiment 5

[0100] (1) Pretreatment of crude alanine fermentation broth,

[0101] 4000L of crude alanine fermentation broth was transferred to a low-temperature static tank at 5.2°C and allowed to stand for 33 hours. The supernatant was removed by siphoning at a flow rate of 8L / min and transferred to a corrosion-resistant container for 16 minutes of ultrasonic treatment at an ultrasonic frequency of 34kHz.

[0102] (2) Nutrient addition and natural fermentation,

[0103] For general crops, 11.5 kg of nitrogen (in terms of ammonium nitrate), 8.2 kg of phosphorus (in terms of potassium dihydrogen phosphate), 9.5 kg of potassium (in terms of potassium sulfate) and trace elements (0.72 kg of iron, 0.42 kg of zinc and 0.26 kg of copper) were added to every 1000 L of fermentation liquid. For 4000 L of fermentation liquid, 46 kg of nitrogen, 32.8 kg of phosphorus, 38 kg of potassium, 2.88 kg of iron, 1.68 kg of zinc and 1.04 kg of copper were added accordingly. The nutrients were added slowly by hand and stirred at a rate of 45 r / min for 13 min with a wooden stick. 0.75 kg of plant growth regulator was added as ammonium ethyl ester and 7×10 Bacillus laterosporus, a beneficial microbial agent, was added to every liter of fermentation liquid. 6 Colony unit, natural fermentation for 40h.

[0104] (3) Solar evaporation concentration,

[0105] A solar evaporation device was built, consisting of a black copper basin, a transparent glass cover and thermal insulation felt. The fermentation liquid was poured into the device and evaporated and concentrated for 4.4 days under sufficient sunlight. The stirring rate was 74r / min, and the mixture was stirred every 1.7h for 9min each time. A condensation recovery device was set up to recover water for replenishing the plant's landscape pool.

[0106] (4) Mixing, molding,

[0107] Rice husk powder accounting for 44% of the volume of the fermentation liquid, the particle size of the rice husk powder was controlled at 36 mesh, and wood cellulose nanoparticles accounting for 8.8% of the volume of the fermentation liquid, the diameter of the wood cellulose nanoparticles was 70nm, and the length was 330nm. The crude fertilizer was pressed into a sphere with a diameter of 2.6cm at a mechanical stirring speed of 102r / min combined with natural wind-assisted stirring for 1.7h.

[0108] (5) Quality monitoring,

[0109] Chemical analysis: pH 7.1;

[0110] The nitrogen content is 4.0%, the phosphorus content is 3.0%, the potassium content is 3.4%, the iron content is 0.20%, the zinc content is 0.13%, and the copper content is 0.1%;

[0111] Physical analysis: The fertilizer particle size is mainly concentrated in 1.4-3.5mm, and the density is 1.27g / cm 3 , the porosity is 48%.

[0112] In the above five groups of embodiments, the specific methods of quality monitoring are as follows:

[0113] pH value detection,

[0114] Test conditions: Use a broad range of pH test paper or a precision pH meter. After the fertilizer is prepared, take a certain amount of fertilizer sample and dissolve it in an appropriate amount of distilled water to form a fertilizer solution.

[0115] Detection method: Use a precision pH meter, insert the pH electrode into the fertilizer solution, wait for the reading to stabilize, record the displayed pH value, and take the average value after multiple measurements.

[0116] Nitrogen, phosphorus, potassium and trace elements content detection:

[0117] (1) Nitrogen content detection (taking Kjeldahl method as an example),

[0118] Testing conditions: Kjeldahl nitrogen determination device is required, including digestion furnace, distillation device, absorption device, and reagents include concentrated sulfuric acid, copper sulfate, sodium hydroxide solution, boric acid solution, etc.

[0119] Detection method: Weigh a certain amount of fertilizer sample and put it into a Kjeldahl flask. Add concentrated sulfuric acid and a catalyst for digestion to convert the organic nitrogen in the fertilizer into ammonium nitrogen. After the digestion is completed, distill it to distill out the ammonium nitrogen, absorb it with boric acid solution, and finally titrate the absorption liquid with standard hydrochloric acid solution. Calculate the nitrogen content based on the amount of hydrochloric acid used. Through multiple parallel experiments, the specific value of the nitrogen content is obtained.

[0120] (2) Phosphorus content detection (taking molybdenum antimony colorimetric method as an example),

[0121] Detection conditions: spectrophotometer, molybdenum antimony anti-color developing agent, related chemical reagents, etc.;

[0122] Detection method: Weigh the fertilizer sample, dissolve it with acid, add molybdenum antimony anti-color developer, make phosphorus react with the color developer to form a blue complex, measure the absorbance of the solution with a spectrophotometer at a specific wavelength, calculate the phosphorus content according to the standard curve, and determine the specific value of the phosphorus content after multiple measurements.

[0123] (3) Potassium content detection (flame photometry as an example),

[0124] Detection conditions: flame photometer, related chemical reagents, etc.;

[0125] Detection method: Dissolve the fertilizer sample to make a test solution, use a flame photometer to measure the intensity of light of a specific wavelength emitted by the potassium element in the solution, determine the potassium content based on the relationship curve between light intensity and potassium content, and take the average value of multiple measurements to obtain the specific value of the potassium content.

[0126] (4) Detection of trace elements (iron, zinc, copper) content (atomic absorption spectrometry as an example), detection conditions: atomic absorption spectrometer, related chemical reagents, etc.;

[0127] Detection method: After the fertilizer sample is digested, it is made into a test solution. An atomic absorption spectrometer is used to measure the absorbance of iron, zinc, and copper elements at specific wavelengths. The content of each element is calculated based on the standard curve. After multiple experiments, the specific values ​​of iron, zinc, and copper are obtained.

[0128] Particle size detection,

[0129] Testing conditions: one set of standard sieves, electronic balance, etc.

[0130] Detection method: Weigh a certain amount of fertilizer sample, sieve it through standard sieves with different apertures, record the mass of fertilizer particles retained on the sieves with different apertures, calculate the proportion of particles in each particle size range, and determine the particle size of the fertilizer after multiple screening experiments.

[0131] Density detection,

[0132] Testing conditions: electronic balance, container of known volume;

[0133] Detection method: Weigh a certain amount of fertilizer sample, put it into a container of known volume, measure the mass and volume of the fertilizer, calculate the density of the fertilizer according to the density formula (density = mass ÷ volume), measure multiple times and take the average value to get the specific value of the density.

[0134] Porosity testing,

[0135] Testing conditions: container of known volume, clean water, electronic balance, etc.

[0136] Detection method: First, measure the volume V1 of the container, put a certain amount of fertilizer sample into the container, then add a known volume of liquid, measure the total volume V2, then weigh the total mass M1 of the fertilizer and liquid in the container, weigh the same volume of liquid mass M2 separately, set the porosity to X, the liquid density to N according to the porosity formula:

[0137]

[0138] The porosity is calculated according to the formula, and the average value is taken from multiple experiments to obtain the specific value of the porosity.

[0139] According to the conclusions drawn from the above five groups of embodiments, the process proposed by the present invention has the advantages of low energy consumption, environmental protection, precise nutrition, reliable quality and wide applicability, etc., which provides an effective way for the sustainable development of agriculture. While meeting the demand of agricultural production for green and efficient fertilizers, it reduces the negative impact on the environment and realizes the efficient utilization of resources.

[0140] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

[0141] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0142] The above contents are merely examples and explanations of the present invention. Various modifications or additions to the specific embodiments described or replacements in similar ways by technicians in the technical field shall fall within the protection scope of the present invention as long as they do not deviate from the invention or exceed the scope defined by the claims.

[0143] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A low-energy green preparation process for alanine fermentation broth fertilizer, including the operation process of pretreatment, preparation, concentration and mixing of alanine fermentation broth, characterized in that: The specific operation steps of the low-energy consumption alanine fermentation liquid fertilizer green preparation process are as follows: Step 1: Pretreatment of crude alanine fermentation broth: transferring the crude alanine fermentation broth into a low-temperature static tank for static treatment, taking the supernatant after impurities are precipitated, and transferring the supernatant into a container for standby use; Step 2: slowly adding nitrogen, phosphorus, potassium and trace elements to the container containing the alanine fermentation liquid, placing the alanine fermentation liquid container in a well-ventilated area, and fermenting naturally; Step 3: Build a solar evaporation device to use the heat of solar energy to slowly evaporate and concentrate the fermentation liquid, and continuously stir the alanine fermentation liquid during the evaporation process; Step 4: Put the concentrated alanine fermentation liquid into a stirring tank, add the filling material and the lignocellulose nanoparticles, and continue stirring to mix. After the stirring is completed, a crude alanine fermentation liquid fertilizer is obtained, and the crude alanine fermentation liquid fertilizer is pressed into a regular shape as required; Step 5: Use chemical and physical analysis methods to test the quality of the crude alanine fermentation broth fertilizer and determine the nutrient content therein.

2. A low-energy consumption green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 1, the alanine fermentation liquid is transferred into a static tank at 4°C-6°C. Each 1000L of alanine fermentation liquid needs to be left to stand for 24h-36h. After the standing is completed, the supernatant in the static tank is taken out by siphoning and transferred into a corrosion-resistant container for standby use. The flow rate should be controlled at 5L / min-10L / min during the siphoning process. After the supernatant is transferred, an ultrasonic treatment step is introduced to treat the alanine fermentation liquid for 10min-20min. After the ultrasonic treatment is completed.

3. A low-energy consumption green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 2, the masses of nitrogen, phosphorus, potassium and trace elements are adjusted according to the nutritional requirements of different crops and the volume of fermentation liquid. The masses of nitrogen, phosphorus, potassium and trace elements added to every 1000L of fermentation liquid are 10kg-15kg, 7kg-10kg, 8kg-12kg and 1kg-2kg, respectively. The trace elements include iron, zinc and copper, and the masses of the three trace elements are 0.5kg-1kg, 0.3kg-0.6kg and 0.2kg-0.4kg, respectively.

4. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 2, the calculated nutrient elements are added to the container containing the alanine fermentation liquid by slow manual addition, and a wooden stick is used for continuous stirring during the addition process. The stirring rate is 30r / min-50r / min, and the stirring time is 10min-15min. Plant growth regulators and beneficial microbial agents are introduced during stirring, and the amount of added bioactive substances is controlled.

5. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step three, a simple solar evaporation device is built, which is mainly composed of a shallow-bottomed black metal container, a transparent glass cover and a heat-insulating material. The alanine fermentation liquid is poured into the solar evaporation device. Under sufficient sunlight, every 1000L of alanine fermentation liquid needs to be evaporated and concentrated for 3d-5d. During the evaporation process, the alanine fermentation liquid is stirred at a stirring rate of 50r / min-80r / min, stirred once every 1h-2h, and stirred for 5min-10min each time.

6. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 4, the concentrated alanine fermentation broth is placed in a stirring tank, and a filler material accounting for 30%-50% of the volume of the alanine fermentation broth and 5%-10% of the volume of the alanine fermentation broth are added. The broth is mixed by a combination of low-speed mechanical stirring and natural wind-assisted stirring. The mechanical stirring speed is controlled between 80 r / min and 120 r / min, and the stirring time is 1 h to 2 h.

7. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 4, the filling material is selected from natural degradable plant fiber powder, specifically one of rice husk powder and wheat bran powder, the particle size of the added filling material is controlled between 20-50 mesh, and the selected wood cellulose nanoparticles have a diameter between 40nm-80nm and a length between 200nm-400nm.

8. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 4, the crude alanine fermentation liquid fertilizer after stirring is pressed into a spherical shape with a diameter of 2cm-3cm.

9. The low-energy green preparation process of alanine fermentation liquid fertilizer according to claim 1, characterized in that: In the step 5, the quality of the crude alanine fermentation broth fertilizer is monitored using chemical and physical analysis methods. The chemical analysis method includes using pH test paper to determine the pH value of the fertilizer and controlling it between 6.5 and 7.

5. At the same time, the chemical titration method is used to determine the nutrient content and monitor the nitrogen, phosphorus, potassium and trace elements to meet the expected standards. The physical analysis method includes determining the parameters of the particle size, density and porosity of the fertilizer.

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