Continuous production process for preparing fertilizer from leucine fermentation liquor

Abstract

The invention discloses a continuous production process for preparing a fertilizer from a leucine fermentation broth, and relates to the technical field of fertilizer preparation, and the continuous production process comprises the steps of pretreatment of the leucine fermentation broth, nutrient element blending, continuous fermentation and quality detection.The continuous production process has the advantages that through the mode of combining high-speed centrifugation and activated carbon adsorption, the quality of the leucine fermentation broth is improved; solid impurities, thallus fragments and the like in the leucine fermentation liquor are effectively removed, the purity of the leucine fermentation liquor is remarkably improved, a high-quality raw material basis is provided for subsequent processing treatment, the stability of the whole production process and the reliability of the product quality are guaranteed, the adverse effects of the impurities on equipment and the reaction process are reduced, and the production cost is reduced. By adding nitrogen, phosphorus, potassium and trace elements, the nutritional ingredients of the fertilizer are more accurate and balanced, the diversified requirements of different crops for nutrients in various growth stages can be met, comprehensive nutrient support is provided for plant growth, and the growth speed, yield and quality of the crops are improved.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, in particular to a continuous production process for preparing fertilizer from leucine fermentation liquid. Background Art

[0002] In the field of modern agriculture, fertilizer plays a vital role in the growth of crops. With the continuous development of science and technology and the pursuit of efficient use of resources, it is increasingly important to explore new fertilizer production processes. Leucine, as an important amino acid, has a wide range of application values ​​in many fields. In the fermentation industry, leucine fermentation broth is a common product in the production of leucine. In the past, the treatment of leucine fermentation broth often faced many problems. On the one hand, most traditional treatment methods did not make full use of the effective ingredients in the fermentation broth. In many cases, the fermentation broth was treated as waste, which not only caused a waste of resources, but also may cause certain pressure on the environment. From the perspective of fertilizer production, although there are many types of fertilizers on the market, there is still room for improvement in terms of the balance of nutrients and efficient absorption and utilization. Applying leucine fermentation liquid to the field of fertilizer preparation is an innovative attempt. Leucine itself contains nutrients such as nitrogen. After proper treatment, it can provide necessary nutrients for plant growth. In addition, the fermentation liquid may also contain some microbial metabolites and other substances that help improve soil structure and soil fertility. However, the existing fertilizer production process mostly adopts intermittent production when processing leucine fermentation broth. This production method has disadvantages such as low production efficiency and unstable product quality. For example, in the intermittent production process, it is difficult to accurately control the process parameters between different batches, resulting in differences in the composition and performance of the produced fertilizers. At the same time, intermittent operation makes the utilization rate of equipment low and increases production costs. Therefore, it is of great practical significance to develop a continuous production process for preparing fertilizers from leucine fermentation broth, which can achieve effective utilization of resources, improve fertilizer production efficiency and ensure the stability of product quality. To this end, we propose a continuous production process for preparing fertilizers from leucine fermentation broth. Summary of the invention

[0003] The object of the present invention is to provide a continuous production process for preparing fertilizer from leucine fermentation liquid.

[0004] In order to solve the problems raised in the above background technology, the present invention provides the following technical solutions: a continuous production process for preparing fertilizer from leucine fermentation broth, comprising pretreatment of leucine fermentation broth, blending of nutrient elements, continuous fermentation and quality inspection operations, wherein the specific operation method of the continuous production process for preparing fertilizer from leucine fermentation broth is as follows: Step 1: pre-treating the leucine fermentation broth by placing it in a high-speed separator for high-speed separation, taking the supernatant and adding activated carbon particles for adsorption, and centrifuging after the adsorption is completed to obtain a clarified leucine fermentation broth pre-treated liquid; Step 2: Send the pre-treated liquid to the blending tank through a corrosion-resistant pipeline, add nitrogen, phosphorus, and potassium source materials according to the established molar ratio to make each element reach the predetermined concentration range, and add a variety of trace elements at the same time. Use a stirring device to stir for a period of time to complete the blending of nutrient elements and obtain a mixed solution; Step 3: Clean and disinfect the reactor in advance, and pump the mixed solution into the reactor through a metering pump and a corrosion-resistant pipe, start the hot water circulation device to heat and control the temperature, then start the stirring device, regularly detect the uniformity of the solution components, and after the components are uniform, monitor with a pH monitor and adjust the pH value to a neutral range with a sodium hydroxide solution to obtain a mixed pre-solution; Step 4: After activating multiple microbial strains respectively, mix them in a specific ratio and place them in a strain storage tank. The storage tank is equipped with a stirring and cooling device to maintain the activity of the strains. Use a sterile pipeline and a metering pump to inoculate the strains into the mixed pre-solution and turn on the pipeline mixer to mix. After the inoculation, the solution flows into a special fermentation pipeline. After the fermentation pipeline is strictly treated, the environmental conditions in the pipeline are controlled to carry out solution fermentation; Step 5: After the fermentation is completed, the liquid fertilizer is transferred to a post-processing device, filtered through three sets of filtering devices, and a preservative and a surfactant are added after the filtration is completed. After the mixing is completed, the liquid fertilizer is activated; Step 6: Conduct quality inspection on the activated liquid fertilizer, fill and bag it continuously after inspection, and store it in a dry and ventilated warehouse.

[0005] As a further solution of the present invention: in the step 1, the leucine fermentation liquid is pumped into a high-speed centrifuge, the centrifugal speed is set to 10000r / min-13000r / min, the centrifuge is started, and the leucine fermentation liquid is centrifuged at a high speed for 20min-30min, solid impurities and bacterial fragments in the leucine fermentation liquid are centrifuged to the edge of the centrifuge, and after the centrifugation is completed, the supernatant in the centrifuge is transferred to a storage tank, and coconut shell activated carbon particles are added thereto for adsorption, the particle size of the coconut shell activated carbon particles is 0.2mm-0.4mm, and the amount of coconut shell activated carbon particles added is 3%-5% of the total mass of the supernatant, stirring is continued during the addition process, the stirring rate is set to 60r / min-80r / min, and stirring and adsorption are continued for 30min-45min. After the adsorption operation is completed, the supernatant with the coconut shell activated carbon particles is transferred to a high-speed centrifuge, the centrifugal speed is set to 8000r / min-10000r / min, and after centrifugation for 15min-20min, a clarified leucine fermentation liquid pretreatment liquid is obtained.

[0006] As a further scheme of the present invention: in the step 2, the leucine fermentation broth pretreatment liquid is transported to the mixing tank through a corrosion-resistant pipeline, and elements are added according to the molar ratio of nitrogen, phosphorus and potassium elements of 4:2:3, the nitrogen source is ammonium nitrate, wherein the nitrogen concentration is 0.4mol / L-0.6mol / L, the phosphorus source is potassium dihydrogen phosphate, wherein the phosphorus concentration is 0.3mol / L-0.5mol / L, and the potassium source is potassium chloride, wherein the potassium concentration is 0.4mol / L-0.6mol / L, and trace elements are added at the same time, including chelated iron, zinc, copper and manganese, and the concentrations thereof are 5mg / L-10mg / L, 3mg / L-8mg / L, 1mg / L-3mg / L and 2mg / L-6mg / L, respectively, and the mixture is stirred by a stirring device at a stirring rate of 100r / min-120r / min for 30min-45min to obtain a mixed solution.

[0007] As a further scheme of the present invention: in the step three, the continuous reactor is pre-cleaned and disinfected to ensure that the interior of the reactor is clean and sterile, the mixed solution is pumped into the continuous reactor through a metering pump and a corrosion-resistant pipe, the hot water circulation device is turned on, the hot water temperature is set between 45°C and 55°C, the temperature of the mixed solution in the reactor is controlled to rise steadily and be maintained in the range of 40°C to 50°C, after the mixed solution is pumped in, the stirring device is started to continuously stir at a stirring rate of 120r / min-150r / min, the solution in the reactor is tested for component uniformity at intervals of 15min, and stirring is continued until the components in the solution are uniformly mixed.

[0008] As a further scheme of the present invention: in the step three, after the components in the mixed solution are stirred evenly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 0.8 mol / L-1.2 mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 5 min-8 min to perform the pH value detection again. When the pH value of the solution is stabilized in the range of 6.5-7.5, pour out to obtain a mixed pre-solution.

[0009] As a further scheme of the present invention: in the step 4, before inoculation, the composite microbial strains are activated and cultured, and Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris are respectively activated in suitable culture media for an activation time of 12h-18h. The activated composite microbial strains are mixed under sterile conditions at a quantity ratio of 3:2:1. The mixing process needs to be carried out in a biological safety cabinet to ensure that the operating environment is sterile. After mixing, the mixed strains are transferred to a strain storage tank, which is equipped with a stirring and cooling device at a stirring speed of 20r / min-30r / min, and the temperature is controlled at 4°C-8°C to maintain the activity of the mixed strains. The strain storage tank is connected to the outlet pipe of the continuous reactor through a sterile pipeline, and a sterile quick connector is used at the pipeline connection. The mixed strains are continuously injected into the mixed pre-solution according to an inoculation amount of 4%-6 of the solution volume using a high-precision metering pump. During the inoculation process, the pipeline mixer is turned on at the same time, and the speed of the mixer is 150r / min-200r / min, so that the strains and the solution are quickly and evenly mixed.

[0010] As a further solution of the present invention: in the step 4, the fermentation pipeline is made of food-grade stainless steel, the inner wall is polished, the roughness is less than 0.4um, the pipeline is spirally coiled, the total length is 30m-50m, the pipeline diameter is 20cm-30cm, and the outside of the pipeline is wrapped with insulation material. The insulation material is polyurethane foam with a thickness of 3cm-5cm to ensure the temperature stability in the pipeline. Before the pipeline is installed, it is strictly cleaned and disinfected, and sterilized with high-pressure steam. The steam pressure is controlled within the range of 0.12MPa-0.15MPa, and the sterilization time lasts for 30min-45min.

[0011] As a further solution of the present invention: in the step 5, the three groups of filtering devices are respectively a primary microfiltration membrane filtering device, a secondary ultrafiltration membrane filtering device and a tertiary nanofiltration membrane filtering device, which respectively select polyvinylidene fluoride microfiltration membrane with a pore size of 0.1um-0.2um, a polyethersulfone ultrafiltration membrane with a pore size of 0.01um-0.03um and a ceramic nanofiltration membrane with a pore size of 0.001um-0.005um, and add a preservative potassium sorbate to the liquid fertilizer after the three groups of filtration, and the addition amount is 0.05%-0.1% of the total mass of the liquid fertilizer. During the addition process , turn on the agitator, the stirring speed is 50r / min-60r / min, the stirring time is 15min-20min, the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, the addition amount is 0.1%-0.3% of the total mass of the liquid fertilizer, and the stirring is continued for 20min-30min, the stirring speed is 60r / min-80r / min, ensure that the surfactant and the fertilizer solution are fully mixed, and use plasma activation treatment equipment to treat the mixed liquid fertilizer to obtain activated liquid fertilizer.

[0012] As a further solution of the present invention: in the step six, the quality inspection includes nutrient component inspection, microbial index inspection and pH value inspection. The nutrient component is inspected by high performance liquid chromatography, the trace elements are inspected by inductively coupled plasma emission spectroscopy, the microbial index is inspected by plate count method, and the pH value of the liquid fertilizer is inspected by a pH meter. After the inspection is qualified, a soft packaging bag made of food-grade plastic material is selected for filling. After the filling is completed, the packaging bag is sealed by a heat sealer, and the packaged liquid fertilizer is transported to a dry and ventilated warehouse for storage.

[0013] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are: 1. The present invention effectively removes solid impurities, bacterial fragments, etc. in leucine fermentation broth by combining high-speed centrifugation and activated carbon adsorption, significantly improves the purity of leucine fermentation broth, provides a high-quality raw material basis for subsequent processing, helps to ensure the stability of the entire production process and the reliability of product quality, reduces the adverse effects of impurities on equipment and reaction processes, and makes the nutritional components of fertilizers more accurate and balanced by adding nitrogen, phosphorus, potassium and trace elements, which can meet the diverse nutritional needs of different crops at various growth stages, provides comprehensive nutrient support for plant growth, and helps to improve the growth rate, yield and Quality. By adding activated cultured composite microbial strains to liquid fertilizer, the liquid fertilizer can have strong activity and function. After mixing them in a specific proportion and inoculating them into the fermentation liquid, a variety of beneficial metabolites are produced during the fermentation process, which can stimulate plant growth, enhance plant disease resistance, and improve soil microecological environment. Special fermentation pipelines and strict environmental control conditions provide an ideal place for microbial fermentation, ensuring that microorganisms can reproduce and metabolize quickly and give full play to their role, further converting organic matter in the fermentation liquid into nutrients that are more easily absorbed and utilized by plants, thereby improving the biological activity and fertilizer efficiency of the fertilizer; 2. The present invention can effectively remove impurities, microbial cells and possible macromolecular harmful substances generated during the fermentation process by using three sets of filtering devices, further improving the purity and stability of the fertilizer, and adding preservatives and surfactants to extend the shelf life of the liquid fertilizer. At the same time, the adhesion and permeability of the fertilizer on the plant surface and in the soil are improved, which helps the fertilizer to play a better role. By using plasma activation treatment on the liquid fertilizer, the molecular structure of the fertilizer is changed, the active groups are increased, the biological activity and absorption utilization rate of the fertilizer are improved, and the fertilizer can provide nutrients to the plants faster and more effectively, thereby enhancing the effect of the fertilizer on promoting crop growth. By using leucine fermentation liquid to prepare fertilizer, the waste of resources caused by the direct disposal of the fermentation liquid is avoided, the leucine and other potentially useful components contained therein are fully retained, the resource utilization rate is improved, and it is in line with the concept of sustainable development. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The figure is a schematic diagram of the process of preparing fertilizer from leucine fermentation broth in an embodiment of the present invention. DETAILED DESCRIPTION

[0015] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0016] The present invention discloses a continuous production process for preparing fertilizer from leucine fermentation liquid, comprising pretreatment of leucine fermentation liquid, blending of nutrient elements, continuous fermentation and quality inspection operations. The specific operation method of the continuous production process for preparing fertilizer from leucine fermentation liquid is as follows: Step 1: pre-treating the leucine fermentation broth by placing it in a high-speed separator for high-speed separation, taking the supernatant and adding activated carbon particles for adsorption, and centrifuging after the adsorption is completed to obtain a clarified leucine fermentation broth pre-treated liquid; Step 2: Send the pre-treated liquid to the blending tank through a corrosion-resistant pipeline, add nitrogen, phosphorus, and potassium source materials according to the established molar ratio to make each element reach the predetermined concentration range, and add a variety of trace elements at the same time. Use a stirring device to stir for a period of time to complete the blending of nutrient elements and obtain a mixed solution; Step 3: Clean and disinfect the reactor in advance, and pump the mixed solution into the reactor through a metering pump and a corrosion-resistant pipe, start the hot water circulation device to heat and control the temperature, then start the stirring device, regularly detect the uniformity of the solution components, and after the components are uniform, monitor with a pH monitor and adjust the pH value to a neutral range with a sodium hydroxide solution to obtain a mixed pre-solution; Step 4: After activating multiple microbial strains respectively, mix them in a specific ratio and place them in a strain storage tank. The storage tank is equipped with a stirring and cooling device to maintain the activity of the strains. Use a sterile pipeline and a metering pump to inoculate the strains into the mixed pre-solution and turn on the pipeline mixer to mix. After the inoculation, the solution flows into a special fermentation pipeline. After the fermentation pipeline is strictly treated, the environmental conditions in the pipeline are controlled to carry out solution fermentation; Step 5: After the fermentation is completed, the liquid fertilizer is transferred to a post-processing device, filtered through three sets of filtering devices, and a preservative and a surfactant are added after the filtration is completed. After the mixing is completed, the liquid fertilizer is activated; Step 6: Conduct quality inspection on the activated liquid fertilizer, fill and bag it continuously after inspection, and store it in a dry and ventilated warehouse.

[0017] In one embodiment of the present invention: in step 1, the leucine fermentation broth is pumped into a high-speed centrifuge, the centrifugal speed is set to 10000r / min-13000r / min, the centrifuge is started, and the leucine fermentation broth is centrifuged at high speed for 20min-30min, solid impurities and bacterial fragments in the leucine fermentation broth are centrifuged to the edge of the centrifuge, and after the centrifugation is completed, the supernatant in the centrifuge is transferred to a storage tank, and coconut shell activated carbon particles are added thereto for adsorption, the particle size of the coconut shell activated carbon particles is 0.2mm-0.4mm, and the amount of coconut shell activated carbon particles added is 3%-5% of the total mass of the supernatant, stirring is continued during the addition process, the stirring rate is set to 60r / min-80r / min, and stirring and adsorption are continued for 30min-45min. After the adsorption operation is completed, the supernatant with the coconut shell activated carbon particles is transferred to a high-speed centrifuge, the centrifugal speed is set to 8000r / min-10000r / min, and after centrifugation for 15min-20min, a clarified leucine fermentation broth pretreatment liquid is obtained.

[0018] In one embodiment of the present invention: in step 2, the leucine fermentation broth pretreatment liquid is transported to a blending tank through a corrosion-resistant pipeline, and elements are added according to a molar ratio of nitrogen, phosphorus and potassium of 4:2:3. The nitrogen source is ammonium nitrate, wherein the nitrogen concentration is 0.4mol / L-0.6mol / L, the phosphorus source is potassium dihydrogen phosphate, wherein the phosphorus concentration is 0.3mol / L-0.5mol / L, and the potassium source is potassium chloride, wherein the potassium concentration is 0.4mol / L-0.6mol / L. At the same time, trace elements are added, including chelated iron, zinc, copper and manganese, and their concentrations are 5mg / L-10mg / L, 3mg / L-8mg / L, 1mg / L-3mg / L and 2mg / L-6mg / L, respectively. The mixture is stirred by a stirring device at a stirring rate of 100r / min-120r / min for 30min-45min to obtain a mixed solution.

[0019] In one embodiment of the present invention: in step three, the continuous reactor is pre-cleaned and disinfected to ensure that the interior of the reactor is clean and sterile, the mixed solution is pumped into the continuous reactor through a metering pump and a corrosion-resistant pipe, the hot water circulation device is turned on, the hot water temperature is set between 45°C and 55°C, the temperature of the mixed solution in the reactor is controlled to rise steadily and be maintained in the range of 40°C to 50°C, after the mixed solution is pumped in, the stirring device is started to continuously stir at a stirring rate of 120r / min-150r / min, the solution in the reactor is tested for component uniformity at intervals of 15min, and stirring is continued until the components in the solution are uniformly mixed.

[0020] In one embodiment of the present invention: in step three, after the components in the mixed solution are stirred evenly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 0.8 mol / L-1.2 mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 5 min-8 min to perform pH detection again. When the pH value of the solution is stabilized in the range of 6.5-7.5, pour out to obtain a mixed pre-solution.

[0021] In one embodiment of the present invention: in step 4, before inoculation, the composite microbial strains are activated and cultured, Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris are activated in suitable culture media respectively, the activation time is 12h-18h, the activated composite microbial strains are mixed under sterile conditions at a quantity ratio of 3:2:1, the mixing process needs to be carried out in a biological safety cabinet to ensure the sterility of the operating environment, after mixing, the mixed strains are transferred to a strain storage tank, the storage tank is equipped with a stirring and cooling device, the stirring speed is 20r / min-30r / min, the temperature is controlled at 4°C-8°C to maintain the activity of the mixed strains, the strain storage tank is connected to the outlet pipe of the continuous reactor through a sterile pipeline, a sterile quick connector is used at the pipeline connection, and a high-precision metering pump is used to continuously inject the mixed strains into the mixed pre-solution according to the inoculation amount of 4%-6 of the solution volume, during the inoculation process, the pipeline mixer is turned on at the same time, and the speed of the mixer is 150r / min-200r / min, so that the strains and the solution are quickly and evenly mixed.

[0022] In one embodiment of the present invention: in step four, the fermentation pipeline is made of food-grade stainless steel, the inner wall is polished, the roughness is less than 0.4um, the pipeline is spirally coiled, the total length is 30m-50m, the pipeline diameter is 20cm-30cm, and the outside of the pipeline is wrapped with insulation material. The insulation material is polyurethane foam with a thickness of 3cm-5cm to ensure that the temperature in the pipeline is stable. Before the pipeline is installed, it is strictly cleaned and disinfected, and sterilized with high-pressure steam. The steam pressure is controlled within the range of 0.12MPa-0.15MPa, and the sterilization time lasts for 30min-45min.

[0023] In one embodiment of the present invention: in step 5, the three groups of filtering devices are respectively a primary microfiltration membrane filtering device, a secondary ultrafiltration membrane filtering device and a tertiary nanofiltration membrane filtering device, which respectively select polyvinylidene fluoride microfiltration membrane with a pore size of 0.1um-0.2um, a polyethersulfone ultrafiltration membrane with a pore size of 0.01um-0.03um and a ceramic nanofiltration membrane with a pore size of 0.001um-0.005um, and add a preservative potassium sorbate to the liquid fertilizer after the three groups of filtration, and the addition amount is 0.05%-0.1% of the total mass of the liquid fertilizer. During the addition process , turn on the agitator, the stirring speed is 50r / min-60r / min, the stirring time is 15min-20min, the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, the addition amount is 0.1%-0.3% of the total mass of the liquid fertilizer, and the stirring is continued for 20min-30min, the stirring speed is 60r / min-80r / min, ensure that the surfactant and the fertilizer solution are fully mixed, and use plasma activation treatment equipment to treat the mixed liquid fertilizer to obtain activated liquid fertilizer.

[0024] In one embodiment of the present invention: in step six, the quality inspection includes nutrient component inspection, microbial index inspection and pH value inspection. The nutrient component is inspected by high performance liquid chromatography, the trace elements are inspected by inductively coupled plasma emission spectroscopy, the microbial index is inspected by plate count method, and the pH value of the liquid fertilizer is inspected by a pH meter. After the inspection is qualified, a soft packaging bag made of food grade plastic material is selected for filling. After the filling is completed, the packaging bag is sealed by a heat sealer, and the packaged liquid fertilizer is transported to a dry and ventilated warehouse for storage.

[0025] In one embodiment of the present invention: after the mixed solution is stirred in the reactor, the pH value of the mixed solution is detected in real time using a pH detector. When the pH value is higher than 7.5, the mixed solution is alkaline, and a dilute sulfuric acid solution with a concentration of 0.6 mol / L-1.0 mol / L is slowly added using a metering pump. The amount of addition is changed according to the mass of the total mixed solution. After each addition of the regulator, stirring is continued for 5 min-8 min, and the pH value is detected again until the pH value of the solution is stabilized between 6.5-7.5, thereby obtaining a mixed pre-solution that meets the requirements.

[0026] In one embodiment of the present invention: controlling the fermentation environment in the pipeline, Temperature control: Set electric heating belts and temperature sensors on the outside of the pipeline to achieve precise temperature control. Wrap the electric heating belts evenly around the pipeline, set a temperature sensor every 2m-3m, and automatically adjust the power of the heating belts according to the data fed back by the sensors to stabilize the temperature inside the pipeline at 30-35℃, and control the temperature fluctuation range within ±0.5℃; Dissolved oxygen control: Use a microporous aeration device to introduce sterile air into the fermentation liquid. The air is filtered and sterilized through a 0.22um filter. According to the dissolved oxygen demand in the fermentation liquid, the dissolved oxygen content is controlled to be between 4mg / L and 6mg / L. At the same time, a dissolved oxygen sensor is installed in the pipeline to collect dissolved oxygen data every 5min-8min, record it, and adjust the fermentation environment according to the dissolved oxygen data; Fermentation time control: Install 6-8 sets of timed sampling valves on the pipeline, and take samples every 1-2 hours to detect the number of microorganisms and the concentration of metabolites. When the number of microorganisms reaches 1×10 8 CFU / mL-5×10 8 CFU / mL, and the concentration of the main metabolites (organic acids, amino acids, etc.) reaches a stable state, the fermentation is completed, and the fermentation time is 24h-36h.

[0027] In one embodiment of the present invention: in the filtering and fermentation operation stage, the fermented liquid fertilizer is pumped through a feed pump at 2m 3 / h-3m 3 / h flow rate pumped into the first-stage microfiltration membrane filtration device, after the first-stage microfiltration of liquid fertilizer into the second-stage ultrafiltration membrane filtration device, the feed pressure is 0.2MPa-0.3MPa, through the circulation pump to make the liquid circulate in the membrane assembly filtration, the circulation flow rate is 3m 3 / h-5m 3 / h. During the ultrafiltration process, the turbidity and macromolecular organic matter content of the filtrate are tested every 20min-30min. When the turbidity of the filtrate is less than 0.5NTU and the removal rate of macromolecular organic matter reaches 80%-90%, the next step of filtration is entered. The secondary ultrafiltration filtrate enters the tertiary nanofiltration membrane filtration device. Under the action of the high-pressure pump, the filtrate is filtered at a speed of 1m3 / h-2m 3 / h flow rate for filtration. During the nanofiltration process, the contents of nutrients and impurity ions in the filtrate are monitored by online conductivity meters and concentration sensors. The operating pressure and flow rate are adjusted according to the monitoring results to effectively concentrate the nutrients. The impurity ion removal rate of the filtered liquid fertilizer reaches 70%-80%.

[0028] Embodiment 1 The leucine fermentation liquid was pumped into a high-speed centrifuge, and the centrifugal speed was set to 10000r / min. After starting, the centrifugation was continued for 30 minutes. After the centrifugation was completed, the supernatant was transferred to a storage tank and 0.2mm coconut shell activated carbon particles were added. The addition amount was 3% of the total mass of the supernatant. During the addition process, the stirring was continued for 45 minutes at a stirring rate of 60r / min. After the adsorption operation was completed, the supernatant with the coconut shell activated carbon particles was transferred to the high-speed centrifuge again, and the centrifugal speed was set to 8000r / min. The centrifugation operation was performed for 20 minutes to obtain a clarified leucine fermentation liquid pretreatment liquid; The pretreatment liquid is transported to the blending tank through a corrosion-resistant pipeline, and elements are added according to the molar ratio of nitrogen, phosphorus and potassium elements of 4:2:3. Ammonium nitrate is selected as the nitrogen source to make the nitrogen concentration reach 0.4 mol / L, potassium dihydrogen phosphate is selected as the phosphorus source to make the phosphorus concentration reach 0.3 mol / L, potassium chloride is selected as the potassium source to make the potassium concentration reach 0.4 mol / L, and trace elements are added at the same time, wherein the concentration of chelated iron is 5 mg / L, the concentration of zinc is 3 mg / L, the concentration of copper is 1 mg / L and the concentration of manganese is 2 mg / L, and the mixture is stirred at a stirring rate of 100 r / min for 45 minutes to complete the nutrient element blending and obtain a mixed solution; The mixed solution is pumped into a continuous reactor through a metering pump and a corrosion-resistant pipe, a hot water circulation device is turned on, and the hot water temperature is set to 45°C. The temperature of the mixed solution in the reactor is controlled to rise steadily and maintained at 40°C. After the mixed solution is pumped in, stirring is continued at a stirring rate of 120r / min. The solution in the reactor is tested for component uniformity every 15 minutes. After the components in the mixed solution are stirred uniformly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 0.8mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 8 minutes to test the pH value again. When the pH value of the solution is stabilized in the range of 6.5-7.5, a mixed pre-solution is obtained; Before inoculation, the composite microbial strains were activated and cultured. Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris were activated in appropriate culture media for 12 hours respectively. The activated composite microbial strains were mixed under sterile conditions at a ratio of 3:2:1. After mixing, the mixed strains were transferred to a strain storage tank. The storage tank was equipped with a stirring and cooling device with a stirring speed of 20r / min and a temperature controlled at 4°C to maintain the activity of the mixed strains. A high-precision metering pump was used to continuously inoculate the mixed strains at an inoculation amount of 4% of the solution volume. The mixture was continuously injected into the mixed pre-solution. During the inoculation process, the pipeline mixer was turned on at the same time. The speed of the mixer was 150r / min. Sterile air was introduced into the fermentation liquid through a microporous aeration device. The air was filtered and sterilized by a 0.22um filter to keep the dissolved oxygen content at 4mg / L. A dissolved oxygen sensor was set in the pipeline to collect dissolved oxygen data every 8min. The fermentation environment was adjusted according to the data. Six sets of timed sampling valves were installed on the pipeline to sample and detect the number of microorganisms and the concentration of metabolites every 2h. When the number of microorganisms reached 1×10 8 CFU / mL-5×10 8 CFU / mL, and the concentration of major metabolites (organic acids, amino acids, etc.) reached a stable state, and the fermentation was completed in 36h; The liquid fertilizer after fermentation is transferred to the post-processing device and pumped at a speed of 2m 3 / h flow rate pumped into the 0.1um polyvinylidene fluoride microfiltration membrane, the liquid fertilizer after the first-level microfiltration enters the second-level ultrafiltration membrane filtration device, the feed pressure is 0.2MPa, the liquid is circulated and filtered in the membrane assembly through the circulation pump, the circulation flow rate is 3m 3 / h, during the ultrafiltration process, the turbidity and macromolecular organic matter content of the filtrate are detected every 20 minutes. When the turbidity of the filtrate is less than 0.5NTU and the removal rate of macromolecular organic matter reaches 80%, the next step of filtration is entered. The secondary ultrafiltration filtrate enters the tertiary nanofiltration membrane filtration device and is filtered at a flow rate of 1m³ / h under the action of a high-pressure pump. During the nanofiltration process, the content of nutrients and impurity ions in the filtrate is monitored by an online conductivity meter and a concentration sensor. The operating pressure and flow rate are adjusted according to the monitoring results to effectively concentrate the nutrients. The impurity ion removal rate of the filtered liquid fertilizer reaches 70%, and the liquid fertilizer is sent to the third stage of the ultrafiltration membrane filtration device. After three groups of filtration, the preservative potassium sorbate is added to the liquid fertilizer in an amount of 0.05% of the total mass of the liquid fertilizer. During the addition process, the agitator is turned on with a stirring speed of 50 r / min and a stirring time of 20 min to evenly disperse the preservative in the fertilizer solution. Then the surfactant alkyl polyglycoside is added in an amount of 0.1% of the total mass of the liquid fertilizer. The stirring is continued for 30 min at a stirring speed of 60 r / min to ensure that the surfactant and the fertilizer solution are fully mixed. Finally, the mixed liquid fertilizer is treated with plasma activation treatment equipment to obtain activated liquid fertilizer.

[0029] Embodiment 2 The leucine fermentation liquid was pumped into a high-speed centrifuge, and the centrifugal speed was set to 11000r / min. After starting, the centrifugation was continued for 28 minutes. After the centrifugation was completed, the supernatant was transferred to a storage tank and coconut shell activated carbon particles with a particle size of 0.3mm were added. The addition amount was 4% of the total mass of the supernatant. During the addition process, the stirring rate was continuously stirred at 70r / min for 40 minutes. After the adsorption operation was completed, the supernatant with the coconut shell activated carbon particles was transferred to the high-speed centrifuge again, and the centrifugal speed was set to 9000r / min. The centrifugation operation was performed for 18 minutes to obtain a clarified leucine fermentation liquid pretreatment liquid; The pretreatment liquid is transported to the blending tank through a corrosion-resistant pipeline, and elements are added according to the molar ratio of nitrogen, phosphorus and potassium elements of 4:2:3. Ammonium nitrate is selected as the nitrogen source to make the nitrogen concentration reach 0.5 mol / L, potassium dihydrogen phosphate is selected as the phosphorus source to make the phosphorus concentration reach 0.4 mol / L, potassium chloride is selected as the potassium source to make the potassium concentration reach 0.5 mol / L, and trace elements are added at the same time, wherein the concentration of chelated iron is 7 mg / L, the concentration of zinc is 5 mg / L, the concentration of copper is 2 mg / L and the concentration of manganese is 4 mg / L, and the mixture is stirred at a stirring rate of 110 r / min for 40 min to complete the nutrient element blending and obtain a mixed solution; The mixed solution is pumped into the continuous reactor through a metering pump and a corrosion-resistant pipe, and the hot water circulation device is turned on, and the hot water temperature is set to 50°C. The temperature of the mixed solution in the reactor is controlled to rise steadily and maintained at 45°C. After the mixed solution is pumped in, it is continuously stirred at a stirring rate of 130r / min. The solution in the reactor is tested for component uniformity every 15 minutes. After the components in the mixed solution are stirred uniformly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 1.0 mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 7 minutes to test the pH value again. When the pH value of the solution is stabilized in the range of 6.5-7.5, a mixed pre-solution is obtained; Before inoculation, the composite microbial strains were activated and cultured. Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris were activated in appropriate culture media for 15 hours respectively. The activated composite microbial strains were mixed under sterile conditions at a ratio of 3:2:1. After mixing, the mixed strains were transferred to a strain storage tank. The storage tank was equipped with a stirring and cooling device with a stirring speed of 25r / min and a temperature controlled at 6°C to maintain the activity of the mixed strains. The mixed strains were continuously inoculated with a high-precision metering pump at an inoculation volume of 5% of the solution volume. The mixture was injected into the pre-solution. During the inoculation process, the pipeline mixer was turned on at the same time. The speed of the mixer was 170r / min. Sterile air was introduced into the fermentation liquid through a microporous aeration device. The air was filtered and sterilized by a 0.22um filter to keep the dissolved oxygen content at 5mg / L. A dissolved oxygen sensor was set in the pipeline to collect dissolved oxygen data every 7 minutes. The fermentation environment was adjusted according to the data. Seven groups of timed sampling valves were installed on the pipeline to sample and detect the number of microorganisms and the concentration of metabolites every 1.5 hours. When the number of microorganisms reached 1×10 8 CFU / mL-5×10 8 CFU / mL, and the concentration of major metabolites (organic acids, amino acids, etc.) reaches a stable state, and the fermentation is completed in 30h; The liquid fertilizer after fermentation is transferred to the post-processing device and pumped at 2.5m 3 / h flow rate pumped into the polyvinylidene fluoride microfiltration membrane with a pore size of 0.15um, after the first-stage microfiltration, the liquid fertilizer enters the second-stage ultrafiltration membrane filtration device, the feed pressure is 0.25MPa, the liquid is circulated and filtered in the membrane assembly through a circulation pump, the circulation flow rate is 4m³ / h, during the ultrafiltration process, the turbidity and macromolecular organic content of the filtrate are tested every 25min, when the turbidity of the filtrate is less than 0.5NTU and the removal rate of macromolecular organic matter reaches 85%, it enters the next step of filtration, the second-stage ultrafiltration filtrate enters the third-stage nanofiltration membrane filtration device, under the action of the high-pressure pump, at a rate of 1.5m 3 / h flow rate for filtration, in the process of nanofiltration, the nutrient content and impurity ion content in the filtrate are monitored by an online conductivity meter and a concentration sensor, the operating pressure and flow rate are adjusted according to the monitoring results, so that the nutrient content is effectively concentrated, and the impurity ion removal rate of the filtered liquid fertilizer reaches 75%, and the preservative potassium sorbate is added to the liquid fertilizer after three groups of filtration, and the added amount is 0.08% of the total mass of the liquid fertilizer. During the adding process, the agitator is turned on, the stirring speed is 55r / min, and the stirring time is 18min, and the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, and the added amount is 0.2% of the total mass of the liquid fertilizer. Stirring is continued for 25min, and the stirring speed is 70r / min to ensure that the surfactant and the fertilizer solution are fully mixed, and finally the mixed liquid fertilizer is treated with a plasma activation treatment equipment to obtain an activated liquid fertilizer.

[0030] Embodiment 3 The leucine fermentation liquid was pumped into a high-speed centrifuge, and the centrifugal speed was set to 12000r / min. After starting, the centrifugation was continued for 26 minutes. After the centrifugation was completed, the supernatant was transferred to a storage tank and coconut shell activated carbon particles with a particle size of 0.35mm were added. The addition amount was 4.5% of the total mass of the supernatant. During the addition process, the stirring was continued for 42 minutes at a stirring rate of 75r / min. After the adsorption operation was completed, the supernatant with the coconut shell activated carbon particles was transferred to the high-speed centrifuge again, and the centrifugal speed was set to 9500r / min. The centrifugation operation was performed for 19 minutes to obtain a clarified leucine fermentation liquid pretreatment liquid; The pretreatment liquid is transported to the blending tank through a corrosion-resistant pipeline, and elements are added according to the molar ratio of nitrogen, phosphorus and potassium elements of 4:2:3. Ammonium nitrate is selected as the nitrogen source to make the nitrogen concentration reach 0.55 mol / L, potassium dihydrogen phosphate is selected as the phosphorus source to make the phosphorus concentration reach 0.45 mol / L, potassium chloride is selected as the potassium source to make the potassium concentration reach 0.5 mol / L, and trace elements are added at the same time, wherein the concentration of chelated iron is 8 mg / L, the concentration of zinc is 6 mg / L, the concentration of copper is 2.5 mg / L and the concentration of manganese is 5 mg / L, and the mixture is stirred at a stirring rate of 115 r / min for 42 minutes to complete the nutrient element blending and obtain a mixed solution; The mixed solution is pumped into a continuous reactor through a metering pump and a corrosion-resistant pipe, and a hot water circulation device is turned on, and the hot water temperature is set to 52°C. The temperature of the mixed solution in the reactor is controlled to rise steadily and maintained at 48°C. After the mixed solution is pumped in, stirring is continued at a stirring rate of 140r / min, and the solution in the reactor is tested for component uniformity at intervals of 15min. After the components in the mixed solution are stirred uniformly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 1.1mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 6min to test the pH value again. When the pH value of the solution is stabilized in the range of 6.5-7.5, a mixed pre-solution is obtained; Before inoculation, the composite microbial strains were activated and cultured. Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris were activated in appropriate culture media for 16 hours respectively. The activated composite microbial strains were mixed under sterile conditions at a ratio of 3:2:1. After mixing, the mixed strains were transferred to a strain storage tank. The storage tank was equipped with a stirring and cooling device with a stirring speed of 28r / min and a temperature controlled at 7°C to maintain the activity of the mixed strains. A high-precision metering pump was used to continuously inject the mixed strains at an inoculation volume of 5.5% of the solution volume. The mixture was added into the pre-solution. During the inoculation process, the pipeline mixer was turned on at the same time. The speed of the mixer was 180r / min. Sterile air was introduced into the fermentation liquid through a microporous aeration device. The air was filtered and sterilized by a 0.22um filter to keep the dissolved oxygen content at 5.5mg / L. A dissolved oxygen sensor was set in the pipeline to collect dissolved oxygen data every 6.5min. The fermentation environment was adjusted according to the data. Seven groups of timed sampling valves were installed on the pipeline to sample and detect the number of microorganisms and the concentration of metabolites every 1.8h. When the number of microorganisms reached 1×10 8 CFU / mL-5×10 8 CFU / mL, and the concentration of major metabolites (organic acids, amino acids, etc.) reaches a stable state, and the fermentation is completed in 28h; The liquid fertilizer after fermentation is transferred to the post-processing device and pumped at 2.8m 3 / h flow rate pumped into the polyvinylidene fluoride microfiltration membrane with a pore size of 0.18um, after the first-stage microfiltration, the liquid fertilizer enters the second-stage ultrafiltration membrane filtration device, the feed pressure is 0.28MPa, the liquid is circulated and filtered in the membrane assembly through a circulation pump, and the circulation flow rate is 4.5m³ / h. During the ultrafiltration process, the turbidity and macromolecular organic content of the filtrate are tested every 28min. When the turbidity of the filtrate is less than 0.5NTU and the removal rate of macromolecular organic matter reaches 88%, it enters the next step of filtration. The second-stage ultrafiltration filtrate enters the third-stage nanofiltration membrane filtration device, and under the action of the high-pressure pump, it is filtered at a rate of 1.8m 3 / h flow rate for filtration, in the process of nanofiltration, the nutrient content and impurity ion content in the filtrate are monitored by an online conductivity meter and a concentration sensor, the operating pressure and flow rate are adjusted according to the monitoring results, so that the nutrient content is effectively concentrated, and the impurity ion removal rate of the filtered liquid fertilizer reaches 78%, and the preservative potassium sorbate is added to the liquid fertilizer after three groups of filtration, and the added amount is 0.09% of the total mass of the liquid fertilizer. During the adding process, the agitator is turned on, the stirring speed is 58r / min, and the stirring time is 19min, and the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, and the added amount is 0.25% of the total mass of the liquid fertilizer. Stirring is continued for 28min, and the stirring speed is 75r / min to ensure that the surfactant and the fertilizer solution are fully mixed, and finally the mixed liquid fertilizer is treated with a plasma activation treatment equipment to obtain an activated liquid fertilizer.

[0031] Embodiment 4: The leucine fermentation liquid was pumped into a high-speed centrifuge, and the centrifugal speed was set to 11500r / min. After starting, the centrifugation was continued for 27 minutes. After the centrifugation was completed, the supernatant was transferred to a storage tank and coconut shell activated carbon particles with a particle size of 0.32mm were added. The addition amount was 4.2% of the total mass of the supernatant. During the addition process, the stirring was continued for 41 minutes at a stirring rate of 72r / min. After the adsorption operation was completed, the supernatant with the coconut shell activated carbon particles was transferred to the high-speed centrifuge again, and the centrifugal speed was set to 9200r / min. The centrifugation operation was performed for 18.5 minutes to obtain a clarified leucine fermentation liquid pretreatment liquid; The pretreatment liquid is transported to the blending tank through a corrosion-resistant pipeline, and elements are added according to the molar ratio of nitrogen, phosphorus and potassium elements of 4:2:3. Ammonium nitrate is selected as the nitrogen source to make the nitrogen concentration reach 0.52 mol / L, potassium dihydrogen phosphate is selected as the phosphorus source to make the phosphorus concentration reach 0.42 mol / L, potassium chloride is selected as the potassium source to make the potassium concentration reach 0.52 mol / L, and trace elements are added at the same time, wherein the concentration of chelated iron is 7.5 mg / L, the concentration of zinc is 5.5 mg / L, the concentration of copper is 2.2 mg / L and the concentration of manganese is 4.5 mg / L, and the mixture is stirred at a stirring rate of 112 r / min for 41 minutes to complete the nutrient element blending and obtain a mixed solution; The mixed solution is pumped into the continuous reactor through a metering pump and a corrosion-resistant pipe, and the hot water circulation device is turned on, and the hot water temperature is set to 51°C. The temperature of the mixed solution in the reactor is controlled to rise steadily and maintained at 47°C. After the mixed solution is pumped in, it is continuously stirred at a stirring rate of 135r / min. The solution in the reactor is tested for component uniformity every 15 minutes. After the components in the mixed solution are stirred uniformly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is higher than 7.5, a dilute sulfuric acid solution with a concentration of 0.8mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 6 minutes to test the pH value again. When the pH value of the solution is stabilized in the range of 6.5-7.5, a mixed pre-solution is obtained; Before inoculation, the composite microbial strains were activated and cultured. Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris were activated in appropriate culture media for 15.5 h, respectively. The activated composite microbial strains were mixed under sterile conditions at a ratio of 3:2:1. After mixing, the mixed strains were transferred to a strain storage tank. The storage tank was equipped with a stirring and cooling device with a stirring speed of 26 r / min and a temperature controlled at 6.5 °C to maintain the activity of the mixed strains. A high-precision metering pump was used to continuously inoculate the mixed strains at an inoculation rate of 5.2% of the solution volume. The mixture was continuously injected into the mixed pre-solution. During the inoculation process, the pipeline mixer was turned on at the same time. The speed of the mixer was 175r / min. Sterile air was introduced into the fermentation liquid through a microporous aeration device. The air was filtered and sterilized by a 0.22um filter to keep the dissolved oxygen content at 5.2mg / L. A dissolved oxygen sensor was set in the pipeline to collect dissolved oxygen data every 6.8min. The fermentation environment was adjusted according to the data. Seven sets of timed sampling valves were installed on the pipeline to sample and detect the number of microorganisms and the concentration of metabolites every 1.6h. When the number of microorganisms reached 1×10 8 CFU / mL-5×10 8 CFU / mL, and the concentration of major metabolites (organic acids, amino acids, etc.) reached a stable state, and the fermentation was completed in 29h; The liquid fertilizer after fermentation is transferred to the post-processing device and pumped at 2.6m 3 / h flow rate pumped into the polyvinylidene fluoride microfiltration membrane with a pore size of 0.16um, the liquid fertilizer after the first-stage microfiltration enters the second-stage ultrafiltration membrane filtration device, the feed pressure is 0.26MPa, the liquid is circulated and filtered in the membrane assembly through a circulation pump, the circulation flow rate is 4.2m³ / h, during the ultrafiltration process, the turbidity and macromolecular organic content of the filtrate are tested every 26min, when the turbidity of the filtrate is less than 0.5NTU and the removal rate of macromolecular organic matter reaches 86%, it enters the next step of filtration, the second-stage ultrafiltration filtrate enters the third-stage nanofiltration membrane filtration device, under the action of the high-pressure pump, at a rate of 1.6m 3 / h flow rate for filtration, in the process of nanofiltration, the nutrient content and impurity ion content in the filtrate are monitored by an online conductivity meter and a concentration sensor, the operating pressure and flow rate are adjusted according to the monitoring results, so that the nutrient content is effectively concentrated, and the impurity ion removal rate of the filtered liquid fertilizer reaches 76%, and the preservative potassium sorbate is added to the liquid fertilizer after three groups of filtration, and the added amount is 0.07% of the total mass of the liquid fertilizer. During the adding process, the agitator is turned on, the stirring speed is 56r / min, and the stirring time is 18.5min, and the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, and the added amount is 0.18% of the total mass of the liquid fertilizer. Stirring is continued for 26min, and the stirring speed is 72r / min to ensure that the surfactant and the fertilizer solution are fully mixed, and finally the mixed liquid fertilizer is treated with a plasma activation treatment equipment to obtain an activated liquid fertilizer.

[0032] The liquid fertilizers prepared in the above four groups of embodiments were tested for quality, and the testing method was as follows: Nutritional testing, Sample pretreatment: Measure 10 mL of the liquid fertilizer to be tested, filter it through a 0.45 um filter membrane, set the mobile phase to a methanol-water system for gradient elution, select a C18 column and set parameters such as column temperature and flow rate, determine the detection wavelength based on the characteristics of the detected components, prepare a standard solution to draw a standard curve, and calculate the nutrient content.

[0033] Trace element detection, Take 5 mL of sample and add dilute nitric acid to digest it, then dilute it to 50 mL. Set the instrument parameters of RF power and gas flow, select the characteristic spectral lines of each element, prepare a series of standard solutions, draw a standard curve, and then conduct detection.

[0034] Microbiological index detection, Prepare the corresponding culture medium and sterilize it, spread the sample on the culture medium plate after gradient dilution, and count after culturing under appropriate conditions.

[0035] pH value detection, Use a pH meter that has been calibrated at three points with a standard pH buffer solution (such as pH 4.00, pH 6.86, and pH 9.18), insert the electrode into the liquid fertilizer sample to be tested, gently stir it to make it uniform, and record the value after the reading stabilizes. Repeat the measurement 3 times for each sample and take the average value.

[0036] The calculation results are as follows: Embodiment 1

[0037] Nutritional ingredients: Leucine content is 1.2g / L, nitrogen content is 40.5g / L, phosphorus content is 30.1g / L, potassium content is 40.2g / L; Trace elements: iron content is 5.2 mg / L, zinc content is 3.1 mg / L, copper content is 1.1 mg / L, and manganese content is 2.2 mg / L; Microbial indicators: The number of Bacillus subtilis is 2.5×10 8 CFU / mL, the number of Lactobacillus acidophilus is 1.8×10 8 CFU / mL, the number of Rhodopseudomonas palustris was 1.2×10 8 CFU / mL; pH: 7.0.

[0038] Embodiment 2 Nutritional composition: Leucine content is 1.4g / L, nitrogen content is 50.2g / L, phosphorus content is 40.3g / L, potassium content is 50.1g / L; Trace elements: iron content is 7.1mg / L, zinc content is 5.3mg / L, copper content is 2.1mg / L, and manganese content is 4.1mg / L; Microbial indicators: The number of Bacillus subtilis is 3.2×10 8 CFU / mL, the number of Lactobacillus acidophilus is 2.3×10 8 CFU / mL, the number of Rhodopseudomonas palustris was 1.5×10 8 CFU / mL; pH: 7.1.

[0039] Embodiment 3 Nutritional ingredients: Leucine content is 1.5g / L, nitrogen content is 55.3g / L, phosphorus content is 45.2g / L, potassium content is 50.5g / L; Trace elements: iron content is 8.3mg / L, zinc content is 6.2mg / L, copper content is 2.4mg / L, and manganese content is 5.1mg / L; Microbial indicators: The number of Bacillus subtilis is 3.8×10 8 CFU / mL, the number of Lactobacillus acidophilus is 2.6×10 8 CFU / mL, the number of Rhodopseudomonas palustris was 1.8×10 8 CFU / mL; pH: 7.2.

[0040] Embodiment 4: Nutritional composition: Leucine content is 1.35g / L, nitrogen content is 52.1g / L, phosphorus content is 42.3g / L, potassium content is 51.8g / L; Trace elements: iron content is 7.6mg / L, zinc content is 5.6mg / L, copper content is 2.3mg / L, and manganese content is 4.6mg / L; Microbial indicators: The number of Bacillus subtilis is 3.5×10 8 CFU / mL, the number of Lactobacillus acidophilus is 2.4×10 8 CFU / mL, the number of Rhodopseudomonas palustris was 1.6×10 8 CFU / mL; pH: 7.1.

[0041] In summary, the feasibility and effectiveness of the continuous production process for preparing fertilizer from leucine fermentation broth in the four groups of embodiments of the present invention have important practical significance and application value.

[0042] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

[0043] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0044] The above contents are merely examples and explanations of the present invention. Various modifications or additions to the specific embodiments described or replacements in similar ways by technicians in the technical field shall fall within the protection scope of the present invention as long as they do not deviate from the invention or exceed the scope defined by the claims.

[0045] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A continuous production process for preparing fertilizer from leucine fermentation broth, comprising pretreatment of leucine fermentation broth, blending of nutrient elements, continuous fermentation and quality inspection operations, characterized in that: The specific operation method of the continuous production process for preparing fertilizer from leucine fermentation liquid is as follows: Step 1, pretreatment of leucine fermentation broth, placing it in a high-speed separator for high-speed separation, adding activated carbon particles for adsorption, and obtaining a clarified leucine fermentation broth pretreatment liquid by centrifugation after adsorption is completed; Step 2: transporting the leucine fermentation broth pretreatment liquid to a blending tank, adding nitrogen, phosphorus, potassium and trace elements, and stirring and mixing to obtain a mixed solution; Step 3, adding the mixed solution into a continuous reactor, controlling the reaction temperature and the stirring rate in the reactor, and adjusting the pH value of the solution to neutral by an acid-base regulator to obtain a mixed pre-solution; Step 4: continuously inoculating the composite microbial strain into the mixed prefabricated liquid through the pipeline, flowing the inoculated solution into the continuous fermentation pipeline, controlling the environmental conditions in the fermentation pipeline, and preparing a fermented liquid solution; Step 5: After the fermentation is completed, the liquid fertilizer is transferred to a post-processing device, filtered through three sets of filtering devices, and a preservative and a surfactant are added after the filtration is completed. After the mixing is completed, the liquid fertilizer is activated; Step 6: Conduct quality inspection on the activated liquid fertilizer, fill and bag it continuously after inspection, and store it in a dry and ventilated warehouse.

2. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step 1, the leucine fermentation liquid is pumped into a high-speed centrifuge, the centrifugal speed is set to 10000r / min-13000r / min, the centrifuge is started, and the leucine fermentation liquid is centrifuged at a high speed for 20min-30min, solid impurities and bacterial fragments in the leucine fermentation liquid are centrifuged to the edge of the centrifuge, and after the centrifugation is completed, the supernatant in the centrifuge is transferred to a storage tank, and coconut shell activated carbon particles are added thereto for adsorption, the coconut shell activated carbon particles have a particle size of 0.2mm-0.4mm, and the amount of coconut shell activated carbon particles added is 3%-5% of the total mass of the supernatant, stirring is continued during the addition process, the stirring rate is set to 60r / min-80r / min, and stirring and adsorption are continued for 30min-45min. After the adsorption operation is completed, the supernatant with the coconut shell activated carbon particles is transferred to a high-speed centrifuge, the centrifugal speed is set to 8000r / min-10000r / min, and after centrifugation for 15min-20min, a clarified leucine fermentation liquid pretreatment liquid is obtained.

3. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step 2, the leucine fermentation broth pretreatment liquid is transported to a blending tank through a corrosion-resistant pipeline, and elements are added according to a molar ratio of nitrogen, phosphorus and potassium of 4:2:

3. Ammonium nitrate is selected as the nitrogen source, wherein the nitrogen concentration is 0.4mol / L-0.6mol / L, potassium dihydrogen phosphate is selected as the phosphorus source, wherein the phosphorus concentration is 0.3mol / L-0.5mol / L, and potassium chloride is selected as the potassium source, wherein the potassium concentration is 0.4mol / L-0.6mol / L. Trace elements are added at the same time, including chelated iron, zinc, copper and manganese, and the concentrations thereof are 5mg / L-10mg / L, 3mg / L-8mg / L, 1mg / L-3mg / L and 2mg / L-6mg / L, respectively. The mixture is stirred by a stirring device at a stirring rate of 100r / min-120r / min for 30min-45min to obtain a mixed solution.

4. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step three, the continuous reactor is pre-cleaned and disinfected to ensure that the interior of the reactor is clean and sterile, the mixed solution is pumped into the continuous reactor through a metering pump and a corrosion-resistant pipe, the hot water circulation device is turned on, the hot water temperature is set between 45°C and 55°C, the temperature of the mixed solution in the reactor is controlled to rise steadily and be maintained in the range of 40°C to 50°C, after the mixed solution is pumped in, the stirring device is started to continuously stir at a stirring rate of 120r / min-150r / min, the solution in the reactor is tested for component uniformity at intervals of 15min, and stirring is continued until the components in the solution are uniformly mixed.

5. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step three, after the components in the mixed solution are stirred evenly, the pH value of the mixed solution is monitored in real time using a pH monitor. When the real-time monitored pH value is lower than 6.5, a sodium hydroxide solution with a concentration of 0.8 mol / L-1.2 mol / L is slowly added using a metering pump. After the sodium hydroxide solution is added, wait for 5 min-8 min to perform the pH value detection again. When the pH value of the solution is stabilized in the range of 6.5-7.5, pour out to obtain a mixed pre-solution.

6. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step 4, before inoculation, the composite microbial strains are activated and cultured, and Bacillus subtilis, Lactobacillus acidophilus and Rhodopseudomonas palustris are activated in suitable culture media respectively, and the activation time is 12h-18h. The activated composite microbial strains are mixed under sterile conditions according to a quantity ratio of 3:2:

1. The mixing process needs to be carried out in a biological safety cabinet to ensure that the operating environment is sterile. After mixing, the mixed strains are transferred to a strain storage tank, and the storage tank is equipped with a stirring and cooling device, the stirring speed is 20r / min-30r / min, and the temperature is controlled at 4°C-8°C to maintain the activity of the mixed strains. The strain storage tank is connected to the outlet pipe of the continuous reactor through a sterile pipeline, and a sterile quick connector is used at the pipeline connection. The mixed strains are continuously injected into the mixed pre-solution according to an inoculation amount of 4%-6 of the solution volume using a high-precision metering pump. During the inoculation process, the pipeline mixer is turned on at the same time, and the speed of the mixer is 150r / min-200r / min, so that the strains and the solution are quickly and evenly mixed.

7. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step 4, the fermentation pipeline is made of food-grade stainless steel, the inner wall is polished, the roughness is less than 0.4um, the pipeline is spirally coiled, the total length is 30m-50m, the pipeline diameter is 20cm-30cm, and the outside of the pipeline is wrapped with insulation material. The insulation material is polyurethane foam with a thickness of 3cm-5cm to ensure the temperature stability in the pipeline. Before the pipeline is installed, it is strictly cleaned and disinfected, and sterilized with high-pressure steam. The steam pressure is controlled within the range of 0.12MPa-0.15MPa, and the sterilization time lasts for 30min-45min.

8. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step 5, the three groups of filtering devices are respectively a first-level microfiltration membrane filtering device, a second-level ultrafiltration membrane filtering device and a third-level nanofiltration membrane filtering device, which respectively select a polyvinylidene fluoride microfiltration membrane with a pore size of 0.1um-0.2um, a polyethersulfone ultrafiltration membrane with a pore size of 0.01um-0.03um and a ceramic nanofiltration membrane with a pore size of 0.001um-0.005um. The preservative potassium sorbate is added to the liquid fertilizer after the three groups of filtration, and the addition amount is 0.05%-0.1% of the total mass of the liquid fertilizer. During the addition process, the agitator is turned on. , stirring speed is 50r / min-60r / min, stirring time is 15min-20min, the preservative is evenly dispersed in the fertilizer solution, and then the surfactant alkyl polyglycoside is added, the addition amount is 0.1%-0.3% of the total mass of the liquid fertilizer, and stirring is continued for 20min-30min, the stirring speed is 60r / min-80r / min, ensure that the surfactant and the fertilizer solution are fully mixed, and use plasma activation treatment equipment to treat the mixed liquid fertilizer to obtain activated liquid fertilizer.

9. The continuous production process for preparing fertilizer from leucine fermentation liquid according to claim 1, characterized in that: In the step six, the quality inspection includes nutrient component inspection, microbial index inspection and pH value inspection. The nutrient component is inspected by high performance liquid chromatography, the trace elements are inspected by inductively coupled plasma emission spectroscopy, the microbial index is inspected by plate count method, and the pH value of the liquid fertilizer is inspected by a pH meter. After the inspection is qualified, a flexible packaging bag made of food-grade plastic material is selected for filling. After the filling is completed, the packaging bag is sealed by a heat sealer, and the packaged liquid fertilizer is transported to a dry and ventilated warehouse for storage.

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