Separation and purification method of hericium erinaceus polysaccharide

Abstract

The invention belongs to the technical field of hericium erinaceus processing, and particularly relates to a hericium erinaceus polysaccharide separation and purification method. A specific buffer solution is selected, so that the pH stability of a reaction system is favorably maintained, and the soybean lecithin and the hericium erinaceus polysaccharide are more stably and firmly combined until the reaction reaches a saturated state. The carrier is added to play a catalytic role, so that the binding efficiency of the soybean lecithin and the hericium erinaceus polysaccharide is accelerated, and the separation and purification time is effectively shortened. Through the synergistic effect of the buffer solution and the carrier, the lipidation effect of the soybean lecithin and the hericium erinaceus polysaccharide can be improved, purification is carried out in a soybean lecithin embedding mode, pollution is avoided, green and environment-friendly effects are achieved, reagent residues are avoided, technological parameters are easy to control, and industrial production can be achieved. Compared with the existing hericium erinaceus polysaccharide content which can only reach 30-60% in the market, the hericium erinaceus polysaccharide content obtained through separation and purification can reach 85% or above.

Description

Technical Field

[0001] The invention belongs to the technical field of Hericium erinaceus processing, and particularly relates to a method for separating and purifying Hericium erinaceus polysaccharide. Background Art

[0002] Hericium erinaceus is a large fleshy mushroom commonly found in the cross section of broad-leaved tree trunks or tree holes. When mature, it turns yellow-brown and fuzzy, very similar to the head of a hairy monkey. It is a precious medicinal and edible fungus. As a medicinal and edible fungus, Hericium erinaceus has always been highly praised by nutritionists and medical scientists, and is a food that is deeply loved by consumers.

[0003] Hericium erinaceus polysaccharide is an effective active ingredient extracted from high-quality Hericium erinaceus fruiting bodies. Hericium erinaceus polysaccharide extracted by hot water has a significant inhibitory effect on mouse sarcoma and Ehrlich ascites cancer. It also has the function of aiding digestion and benefiting the five internal organs. It has a good therapeutic effect on various digestive tract diseases such as chronic gastritis and duodenal ulcers, and can improve human immunity. It also has anti-ulcer and anti-inflammatory, liver protection, anti-aging effects, can improve the body's ability to tolerate hypoxia, increase cardiac blood output, accelerate the body's blood circulation, and reduce blood sugar and blood lipids.

[0004] At present, a variety of methods for extracting Hericium erinaceus polysaccharides have been developed, such as hot water extraction, acid extraction, alkali extraction, enzyme extraction, etc. Among them, acid extraction and alkali extraction have high polysaccharide extraction rates, but have high requirements for equipment, and acid and alkali conditions can easily destroy the molecular structure of polysaccharides; hot water extraction is the most commonly used, but the polysaccharide yield obtained by single hot water extraction is low, and long-term high-temperature treatment may destroy the molecular structure of polysaccharides; enzyme extraction reaction conditions are relatively mild, and the polysaccharide yield is relatively high, but the cost of enzyme preparations is high, which limits its large-scale application. Summary of the invention

[0005] The present invention aims to overcome the defects of the prior art of Hericium erinaceus polysaccharide extraction process, such as high cost, low polysaccharide extraction rate and easy destruction of the molecular structure of the polysaccharide, and provides a method for separating and purifying Hericium erinaceus polysaccharide.

[0006] To achieve the above-mentioned purpose, the present invention is implemented by the following technical solutions: A method for separating and purifying Hericium erinaceus polysaccharide comprises the following steps: (S.1) adding water to the raw material of Hericium erinaceus and heating and stirring to extract and obtain a crude Hericium erinaceus product; (S.2) placing the crude Hericium erinaceus obtained in step (S.1) in a buffer solution, adding a carrier, mixing evenly, then adding soybean lecithin, controlling the speed and performing a lipidation reaction under heat preservation conditions, cooling after the reaction is complete, filtering and drying the precipitate to obtain lipidated Hericium erinaceus polysaccharide; (S.3) Add the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) into an ethanol solution, filter and dry the precipitate to obtain a refined Hericium erinaceus polysaccharide.

[0007] Soybean lecithin is an amphiphilic substance, and its molecular structure has one end that is hydrophilic and the other end that is lipophilic. Under the synergistic effect of the buffer solution and the carrier, the hydrophilic end of soybean lecithin is easily combined with Hericium erinaceus polysaccharide, so that soybean lecithin can wrap the Hericium erinaceus polysaccharide molecules, while the lipophilic end of soybean lecithin is exposed to the outside, causing it to be insoluble in water, forming a precipitate, and obtaining lipidated Hericium erinaceus polysaccharide. Then the lipidated Hericium erinaceus polysaccharide is put into an ethanol solution, and since soybean lecithin is easily soluble in the ethanol solution, the Hericium erinaceus polysaccharide is released, and finally the effect of separation and purification is achieved.

[0008] The present invention uses a specific buffer solution, which helps to maintain the pH stability of the reaction system and promotes the combination of soybean lecithin and Hericium erinaceus polysaccharide to be more stable and firm until the reaction reaches a saturated state. The present invention plays a catalytic role by adding a carrier, which helps to accelerate the combination efficiency of soybean lecithin and Hericium erinaceus polysaccharide and effectively shorten the separation and purification time. The present invention helps to improve the lipidation effect of soybean lecithin and Hericium erinaceus polysaccharide through the synergistic effect of the buffer solution and the carrier. The present invention adopts the soybean lecithin embedding method for purification, which is pollution-free, green and environmentally friendly, has no reagent residue, and the process parameters are easy to control, which helps to achieve industrial production. Compared with the Hericium erinaceus polysaccharide content that can only reach 30-60% on the current market, the Hericium erinaceus polysaccharide content obtained by separation and purification of the present invention can reach more than 85%.

[0009] Preferably, the mass ratio of the Hericium erinaceus raw material to water added in step (S.1) is 1:8-12.

[0010] As a further preference, the mass ratio of the Hericium erinaceus raw material to water added in step (S.1) is 1:10.

[0011] Preferably, the extraction temperature during the heating and stirring process in step (S.1) is 70 to 90°C, and the extraction time is 1 to 3 hours.

[0012] As a further preference, the extraction temperature in the heating and stirring process in step (S.1) is 80°C, and the extraction time is 2 hours.

[0013] Preferably, the buffer solution in step (S.2) is any one or more combinations of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, acetic acid-sodium acetate buffer solution, and sodium bicarbonate-sodium carbonate buffer solution.

[0014] As further preferred, the buffer solution in step (S.2) is a sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution.

[0015] Preferably, the pH of the buffer solution in step (S.2) is 7-8.

[0016] As further preferred, the pH of the buffer solution in step (S.2) is 7.5.

[0017] Preferably, the carrier in step (S.2) is any one or more combinations of calcium chloride, magnesium chloride and silicon dioxide.

[0018] As further preferred, the carrier in step (S.2) is calcium chloride.

[0019] Preferably, the amount of the carrier added in step (S.2) is 0.5 to 1.5% of the mass of the Hericium erinaceus raw material.

[0020] As further preferred, the amount of the carrier added in step (S.2) is 1% of the mass of the Hericium erinaceus raw material.

[0021] Preferably, the mass ratio of the crude Hericium erinaceus to soybean lecithin added in step (S.2) is 1:2-4.

[0022] As a further preference, the mass ratio of the crude Hericium erinaceus to soybean lecithin added in step (S.2) is 1:3.

[0023] Preferably, the lipidation reaction process in step (S.2) is carried out at a holding temperature of 40 to 60°C, a controlled rotation speed of 6000 to 10000 r / min, and a reaction time of 1 to 3 h.

[0024] As a further preference, the lipidation reaction process in step (S.2) is carried out at a holding temperature of 50°C, a controlled rotation speed of 8000 r / min, and a reaction time of 2 h.

[0025] Preferably, the reaction temperature in the cooling process in step (S.2) is 0 to 8°C, and the reaction time is 2 to 6 hours.

[0026] As a further preference, the reaction temperature of the cooling process in step (S.2) is 4°C and the reaction time is 4h.

[0027] Preferably, the mass ratio of lipidated Hericium erinaceus polysaccharide to ethanol solution added in step (S.3) is 1:4-6.

[0028] As a further preference, the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution added in step (S.3) is 1:5.

[0029] Therefore, the present invention has the following beneficial effects: (1) The present invention uses a specific buffer solution, which helps to maintain the pH stability of the reaction system and promotes the combination of soybean lecithin and Hericium erinaceus polysaccharide to be more stable and firm until the reaction reaches a saturated state; (2) The present invention plays a catalytic role by adding a carrier, which helps to accelerate the binding efficiency of soybean lecithin and Hericium erinaceus polysaccharide and effectively shorten the separation and purification time; (3) The present invention helps to improve the lipidation effect of soybean lecithin and Hericium erinaceus polysaccharide through the synergistic effect of the buffer solution and the carrier, and purifies by soybean lecithin embedding, which is pollution-free, green and environmentally friendly, has no reagent residue, and the process parameters are easy to control, which helps to achieve industrial production; (4) Compared with the Hericium erinaceus polysaccharide content of only 30-60% in the current market, the Hericium erinaceus polysaccharide content separated and purified by the present invention can reach more than 85%. DETAILED DESCRIPTION

[0030] The present invention is further described below in conjunction with specific embodiments. Those of ordinary skill in the art will be able to implement the present invention based on these descriptions. In addition, the embodiments of the present invention involved in the following description are generally only embodiments of a part of the present invention, rather than all embodiments. Therefore, based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work should fall within the scope of protection of the present invention.

[0031] Example 1 This embodiment provides a method for separating and purifying Hericium erinaceus polysaccharide, comprising the following steps: (S.1) adding water to the raw material of Hericium erinaceus (the mass ratio of the raw material of Hericium erinaceus to water is 1:10), stirring and heating to 80°C for extraction for 2 hours, extracting twice, combining the extracts, filtering to remove impurities, and then precipitating with alcohol, filtering and drying to obtain a crude Hericium erinaceus product; (S.2) placing the crude Hericium erinaceus obtained in step (S.1) in a sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution at pH = 7.5, adding calcium chloride (the amount of calcium chloride added is 1% of the mass of the Hericium erinaceus raw material), mixing evenly, and then adding soybean lecithin (the mass ratio of the crude Hericium erinaceus to soybean lecithin is 1:3), controlling the speed to 8000r / min, keeping the temperature at 50°C for lipidation reaction for 2h, cooling to 4°C after the reaction is complete, keeping the temperature for precipitation for 4h, filtering and drying the precipitate to obtain lipidated Hericium erinaceus polysaccharide; (S.3) Add the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) into a 95% ethanol solution (the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution is 1:5), filter and dry the precipitate to obtain Hericium erinaceus polysaccharide concentrate.

[0032] Example 2 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the mass ratio of Hericium erinaceus raw material to water added in step (S.1) is 1:8. Others are the same as in Example 1.

[0033] Example 3 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the mass ratio of Hericium erinaceus raw material to water added in step (S.1) is 1:12. Others are the same as in Example 1.

[0034] Example 4 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 70°C and stirred for extraction for 2 hours. The rest is the same as in Example 1.

[0035] Example 5 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 90°C and stirred for extraction for 2 hours. The rest is the same as in Example 1.

[0036] Example 6 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 80°C and stirred for extraction for 1 hour. The rest is the same as in Example 1.

[0037] Example 7 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 80°C and stirred for extraction for 3 hours. The rest is the same as in Example 1.

[0038] Comparative Example 1 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the mass ratio of Hericium erinaceus raw material to water added in step (S.1) is 1:6. Others are the same as in Example 1.

[0039] Comparative Example 2 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the mass ratio of Hericium erinaceus raw material to water added in step (S.1) is 1:14. Others are the same as in Example 1.

[0040] Comparative Example 3 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 60°C and stirred for extraction for 2 hours. The rest is the same as in Example 1.

[0041] Comparative Example 4 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 100° C. and extracted for 2 h with stirring. The rest is the same as in Example 1.

[0042] Comparative Example 5 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 80°C and stirred for extraction for 0.5 h. Other aspects are the same as those in Example 1.

[0043] Comparative Example 6 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.1), the mixture is heated to 80°C and stirred for 4 hours. The rest is the same as in Example 1.

[0044]

Extraction process of crude Hericium erinaceus

[0045] Table 1

[0046] From the data analysis in Table 1, it can be seen that the best extraction process of Hericium erinaceus crude product is 10 times the amount of water of Hericium erinaceus raw material, 80℃ extraction for 2h, extraction twice, and the extraction rate can reach more than 98%. At this time, the dry powder content after alcohol precipitation and drying is about 30%.

[0047] Comparative Example 7 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the crude Hericium erinaceus obtained in step (S.1) is placed in water. Other steps are the same as those in Example 1.

[0048] Comparative Example 8 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein calcium chloride is not added in step (S.2). Other steps are the same as those in Example 1.

[0049]

Determination of lipid buffer solution system and carrier

[0050] Table 2 Group Yield of Hericium erinaceus polysaccharide (%) Example 1 85.75 Comparative Example 7 31.18 Comparative Example 8 71.87 .

[0051] From the data analysis in Table 2, it can be seen that the sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution system and the addition of calcium chloride as a carrier are more conducive to the lipidation of Hericium erinaceus polysaccharide.

[0052] Example 8 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 40°C for 2 hours. The rest is the same as in Example 1.

[0053] Example 9 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 60°C for 2 hours. The rest is the same as in Example 1.

[0054] Example 10 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the rotation speed in step (S.2) is controlled to be 6000 r / min. Other conditions are the same as those in Example 1.

[0055] Embodiment 11 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the rotation speed in step (S.2) is controlled to be 10000 r / min. Other conditions are the same as those in Example 1.

[0056] Example 12 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 50°C for 1 hour. The rest is the same as in Example 1.

[0057] Embodiment 13 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 50°C for 3 hours. The rest is the same as in Example 1.

[0058] Embodiment 14 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein soybean lecithin is added in step (S.2) (the mass ratio of crude Hericium erinaceus to soybean lecithin is 1:2). Other aspects are the same as those in Example 1.

[0059] Embodiment 15 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein soybean lecithin is added in step (S.2) (the mass ratio of crude Hericium erinaceus to soybean lecithin is 1:4). Other aspects are the same as those in Example 1.

[0060] Comparative Example 9 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 35°C for 2 hours. The rest is the same as in Example 1.

[0061] Comparative Example 10 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 65°C for 2 hours. The rest is the same as in Example 1.

[0062] Comparative Example 11 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the rotation speed in step (S.2) is controlled to be 4000 r / min. Other steps are the same as those in Example 1.

[0063] Comparative Example 12 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein the rotation speed in step (S.2) is controlled to be 12000 r / min. Other steps are the same as those in Example 1.

[0064] Comparative Example 13 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 50°C for 0.5h. The rest is the same as in Example 1.

[0065] Comparative Example 14 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), the lipidation reaction is carried out at 50°C for 3.5 hours. The rest is the same as in Example 1.

[0066] Comparative Example 15 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein soybean lecithin is added in step (S.2) (the mass ratio of crude Hericium erinaceus to soybean lecithin is 1:1). Other aspects are the same as those in Example 1.

[0067] Comparative Example 16 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein soybean lecithin is added in step (S.2) (the mass ratio of crude Hericium erinaceus to soybean lecithin is 1:5). Other aspects are the same as those in Example 1.

[0068] Determination of lipid parameters Lipidated Hericium erinaceus polysaccharides were prepared according to the methods in Example 1, Examples 8 to 15 and Comparative Examples 9 to 16, and their yields were determined. The results are shown in Table 3 below.

[0069] Table 3 Group Yield of Hericium erinaceus polysaccharide (%) Example 1 85.75 Example 8 80.18 Example 9 81.23 Example 10 80.79 Embodiment 11 83.98 Example 12 80.59 Embodiment 13 83.37 Embodiment 14 82.17 Embodiment 15 84.16 Comparative Example 9 71.19 Comparative Example 10 72.31 Comparative Example 11 70.84 Comparative Example 12 71.95 Comparative Example 13 71.28 Comparative Example 14 73.55 Comparative Example 15 72.43 Comparative Example 16 73.11 .

[0070] From the data analysis in Table 3, it can be seen that the optimal lipid conditions are as follows: the insulation temperature is 50°C, the amount of soybean lecithin is 3 times the quality of the crude Hericium erinaceus product, the control speed is 8000r / min, and the lipid reaction time is 2h.

[0071] Example 16 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 0°C and kept warm for precipitation for 4 hours. The rest is the same as in Example 1.

[0072] Embodiment 17 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 8°C and kept warm for 4 hours for precipitation. The rest is the same as in Example 1.

[0073] Embodiment 18 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 4°C and kept warm for precipitation for 2 hours. The rest is the same as in Example 1.

[0074] Embodiment 19 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 4°C and kept warm for precipitation for 6 hours. The rest is the same as in Example 1.

[0075] Comparative Example 17 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 12° C. and kept warm for 4 hours for precipitation. The rest is the same as in Example 1.

[0076] Comparative Example 18 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 4°C and kept warm for precipitation for 1 hour. The rest is the same as in Example 1.

[0077] Comparative Example 19 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.2), after the reaction is complete, the temperature is lowered to 4°C and the temperature is kept for precipitation for 7 hours. The rest is the same as in Example 1.

[0078] Selection of lipid precipitation parameters Lipidated Hericium erinaceus polysaccharides were prepared according to the methods in Example 1, Examples 16 to 19 and Comparative Examples 17 to 19, and their yields were determined. The results are shown in Table 4 below.

[0079] Table 4 Group Yield of Hericium erinaceus polysaccharide (%) Example 1 85.75 Example 16 84.12 Embodiment 17 80.21 Embodiment 18 81.55 Embodiment 19 83.25 Comparative Example 17 74.12 Comparative Example 18 71.55 Comparative Example 19 73.28 .

[0080] From the data analysis in Table 4, it can be seen that the optimal conditions for lipid precipitation are a reaction temperature of 4°C and a precipitation reaction time of 4h during the cooling precipitation process.

[0081] Embodiment 20 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.3), the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) is added to a 95% ethanol solution (the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution is 1:4). Others are the same as in Example 1.

[0082] Embodiment 21 The difference between this embodiment and embodiment 1 is that: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.3), the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) is added to a 95% ethanol solution (the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution is 1:6). Others are the same as in Example 1.

[0083] Comparative Example 20 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.3), the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) is added to a 95% ethanol solution (the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution is 1:3). Others are the same as in Example 1.

[0084] Comparative Example 21 The difference between this comparative example and Example 1 is: A method for separating and purifying Hericium erinaceus polysaccharide, wherein in step (S.3), the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) is added to a 95% ethanol solution (the mass ratio of the lipidated Hericium erinaceus polysaccharide to the ethanol solution is 1:7). Others are the same as in Example 1.

[0085]

Purification conditions determined

[0086] Table 5 Group Yield of fine Hericium erinaceus polysaccharide (%) Hericium erinaceus polysaccharide content (%) Example 1 85.75 87.24 Embodiment 20 81.24 83.66 Embodiment 21 82.88 85.21 Comparative Example 20 72.59 74.89 Comparative Example 21 73.71 75.64 .

[0087] From the data analysis in Table 5, it can be seen that when the mass of the added 95% ethanol solution is 5 times the mass of the lipidated Hericium erinaceus polysaccharide, the purification effect is the best.

[0088] The above description is only a detailed description of the preferred embodiments and principles of the present invention. For ordinary technicians in this field, according to the ideas provided by the present invention, there will be changes in the specific implementation methods, and these changes should also be regarded as the protection scope of the present invention.

Claims

1. A method for separating and purifying Hericium erinaceus polysaccharide, characterized in that: The following steps are involved: (S.1) adding water to the raw material of Hericium erinaceus and heating and stirring to extract and obtain a crude Hericium erinaceus product; (S.2) placing the crude Hericium erinaceus obtained in step (S.1) in a buffer solution, adding a carrier, mixing evenly, then adding soybean lecithin, controlling the speed and performing a lipidation reaction under heat preservation conditions, cooling after the reaction is complete, filtering and drying the precipitate to obtain lipidated Hericium erinaceus polysaccharide; (S.3) Add the lipidated Hericium erinaceus polysaccharide obtained in step (S.2) into an ethanol solution, filter and dry the precipitate to obtain a refined Hericium erinaceus polysaccharide.

2. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The mass ratio of the Hericium erinaceus raw material to water added in step (S.1) is 1:8-12.

3. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The extraction temperature during the heating and stirring process in step (S.1) is 70 to 90° C., and the extraction time is 1 to 3 hours.

4. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The buffer solution in step (S.2) is any one or more combinations of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, acetic acid-sodium acetate buffer solution, and sodium bicarbonate-sodium carbonate buffer solution.

5. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The carrier in step (S.2) is any one or more combinations of calcium chloride, magnesium chloride and silicon dioxide.

6. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1 or 5, characterized in that: The amount of the carrier added in step (S.2) is 0.5-1.5% of the mass of the Hericium erinaceus raw material.

7. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The mass ratio of the crude Hericium erinaceus product to soybean lecithin added in step (S.2) is 1:2-4.

8. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The lipidation reaction process in step (S.2) is carried out at a holding temperature of 40 to 60°C, a controlled rotation speed of 6000 to 10000 r / min, and a reaction time of 1 to 3 h.

9. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The reaction temperature of the cooling process in step (S.2) is 0 to 8°C, and the reaction time is 2 to 6 hours.

10. The method for separating and purifying Hericium erinaceus polysaccharide according to claim 1, characterized in that: The mass ratio of the lipidated Hericium erinaceus polysaccharide added in step (S.3) to the ethanol solution is 1:4-6.

Images