Machine-washing dishwashing liquid composition containing sophorolipid as well as preparation method and application of machine-washing dishwashing liquid composition

Abstract

The invention discloses a machine-washing dishwashing liquid composition containing sophorolipid as well as a preparation method and application thereof, and relates to the technical field of daily chemical products. The machine-washing dishwashing liquid composition is prepared from the following components in percentage by mass: 1 to 5 percent of sophorolipid or high-acid type sophorolipid, 0.3 to 5 percent of biological enzyme, 2 to 15 percent of alcohol solvent, 5 to 20 percent of strong alkali aid, 0.02 to 2 percent of pH regulator and 10 to 20 percent of chelating agent. The machine-washing dishwashing liquid composition containing sophorolipid has excellent detergency, is green and low-carbon, has efficient and stable enzyme activity in the composition, has good foamability, is easy to rinse, and has extremely low cytotoxicity. The invention discloses novel application of sophorolipid, sophorolipid or high-acid sophorolipid is used as a surfactant, so that the stability and decontamination effect of biological enzyme in a machine-washing dishwashing liquid composition system can be improved, and the sophorolipid or the high-acid sophorolipid also has the benefits of being easy to rinse and enabling the composition to be milder.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and more specifically relates to a machine-washing dishwashing liquid composition containing sophorolipids, and a preparation method and application thereof. Background Art

[0002] Dishwashers have greatly reduced the time and physical labor people spend on dishwashing, and are a huge potential incremental market in China. Therefore, it is very necessary to develop new special products that match the usage scenarios of dishwashers. In addition to the basic degreasing function of traditional dishwashing detergents, machine dishwashing liquids must also have higher protein and starch dirt removal functions, fast foaming but easy rinsing, and mild and non-toxic characteristics.

[0003] However, the current dishwashing liquid system has the following three main problems: 1) Both proteases and amylases have poor stability in dishwashing detergent systems; 2) Traditional surfactants have good foaming properties, but they will cause certain damage to the dishwasher and are not easy to rinse, and surfactants with poor foaming properties are easy to rinse but often have weak decontamination capabilities; 3) The mild and non-toxic properties of the product need to be improved. Therefore, it is necessary to develop a machine-washing dishwashing liquid that can solve the above problems to meet the use requirements. Based on this, the present invention provides a machine-washing dishwashing liquid composition containing sophorolipids, and a preparation method and application thereof. Summary of the invention

[0004] In order to overcome the shortcomings and deficiencies of the prior art, the present invention provides a machine dishwashing liquid composition containing sophorolipids and a preparation method and application thereof. The machine dishwashing liquid composition has excellent decontamination ability, good stability, good foam, easy rinsing, extreme mildness and extremely low cytotoxicity.

[0005] Another object of the present invention is to provide a method for preparing a machine dishwashing liquid composition containing sophorolipids.

[0006] Another object of the present invention is to provide a use of a sophorolipid or a high-acid sophorolipid as an additive for improving the stability of an enzyme preparation in a dishwashing liquid composition system.

[0007] Another object of the present invention is to provide a sophorolipid or a high-acid sophorolipid for use in improving the mildness of a dishwashing liquid composition system and reducing the cytotoxicity of the composition.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] In a first aspect, a machine dishwashing liquid composition containing sophorolipids is provided, comprising the following components in percentage by weight:

[0010] Sophorolipid or high-acid sophorolipid 1-5%, biological enzyme 0.3-5%, alcohol solvent 2-15%, strong alkali auxiliary agent 5-20%, pH regulator 0.02-2%, chelating agent 10-20%.

[0011] Among them, sophorolipids are mainly used in personal care products, and their application in household cleaning products is still very rare. Sophorolipids are generally used as surfactants and moisturizing ingredients, and there are no reports on their use in machine dishwashing liquids and improving the stability of enzyme preparations in machine dishwashing liquid compositions. The present invention adds sophorolipids to a dishwashing liquid composition whose main components are a strong alkali auxiliary and a chelating agent. Sophorolipids or high-acid sophorolipids (the weight of the acid-type structure accounts for >80%) can improve the stability and decontamination ability of the enzyme preparation (biological enzyme) in the dishwashing liquid composition. The prepared dishwashing liquid composition foams quickly and is easy to defoam and rinse, greatly improving the mildness of the dishwashing liquid composition.

[0012] Among them, compared with conventional sophorolipid raw materials, high-acid sophorolipids have a better stabilizing effect on the enzyme preparations (biological enzymes) in the system, making the system milder and easier to rinse.

[0013] Furthermore, the biological enzyme is selected from one or more of proteases and amylases.

[0014] Furthermore, the mass percentage of the protease in the machine-washing dishwashing liquid composition containing sophorolipids is 0.5-3%, and the mass percentage of the amylase in the machine-washing dishwashing liquid composition containing sophorolipids is 0.3-2%.

[0015] Among them, sophorolipids or high-acid sophorolipids can improve the stability and detergency of proteases and some amylases in dishwashing liquid compositions. Sophorolipids also improve the mildness of dishwashing liquid compositions and reduce the cytotoxicity of dishwashing liquid compositions.

[0016] Furthermore, the alcohol solvent includes one or more of glycerol and propylene glycol.

[0017] Furthermore, the strong base auxiliary agent is heavy soda ash.

[0018] Furthermore, the pH regulator is citric acid.

[0019] The role of the chelating agent is to eliminate the influence of metal ions in water through chelation. The chelating agent can be a chelating agent commonly used in the art.

[0020] Furthermore, the chelating agent includes one or more of glutamic acid diacetate sodium salt, sodium citrate, and methylglycine diacetate trisodium salt.

[0021] Furthermore, the sophorolipid-containing machine dishwashing liquid composition also includes a suspending agent and a preservative, and the suspending agent includes one or more of xanthan gum, cross-linked polyacrylic acid salt, and cross-linked polyacrylic acid ester.

[0022] Furthermore, the machine washing dishwashing liquid composition containing sophorolipids is composed of the following components in mass percentage: sophorolipids or high-acid sophorolipids 1-5%, biological enzymes 0.3-5%, alcohol solvents 2-15%, strong alkali additives 5-20%, pH regulators 0.02-2%, chelating agents 10-20%, preservatives 0.01-0.1%, suspending agents 0.1-1.0%, and the balance is water.

[0023] The sophorolipids described in the present invention are a type of secondary metabolites with surface activity, which are synthesized by non-pathogenic Candida albicans using C12-C22 fatty acids / oils and glucose as substrates, and the weight proportion of the acid-type structure in the product is between 40-55%; the high-acid sophorolipids refer to sophorolipids synthesized by fermentation of the engineered strains in the Chinese patent CN115851472A using C12-C22 fatty acids / oils and glucose as substrates, and the weight proportion of the acid-type structure is greater than 80%.

[0024] The function of the preservative is to prevent bacteria from growing and causing the detergent to spoil and deteriorate. The preservative can be a preservative commonly used in the art.

[0025] Furthermore, the preservative is one or more of benzisothiazolinone, methylisothiazolinone, and methylchloroisothiazolinone.

[0026] In a second aspect, a method for preparing the machine dishwashing liquid composition containing sophorolipids as described in the first aspect is provided, comprising the following steps:

[0027] S1. The chelating agent, alcohol solvent and suspending agent are mixed and dispersed evenly until there are no particles and clumps to obtain a mixture A; water is added to the mixture A and stirring is started;

[0028] S2. Add a strong base additive, sophorolipid or high-acid sophorolipid and stir evenly;

[0029] S3. Add a pH adjuster to adjust the pH to 6.8-8.0, then add a biological enzyme and a preservative, and stir evenly to obtain a machine-washing dishwashing liquid composition containing sophorolipids.

[0030] In a third aspect, there is provided a use of the machine-washing dishwashing liquid composition containing sophorolipids as described in the first aspect in the preparation of a special detergent for a dishwasher.

[0031] The present invention provides a machine dishwashing liquid composition containing sophorolipids, and a preparation method and application thereof. Compared with the existing technical solutions, the machine dishwashing liquid composition of the present invention has the following advantages:

[0032] The present invention discloses a new application of sophorolipids. Sophorolipids or sophorolipids as surfactants can not only improve the stability and decontamination effect of enzyme preparations (biological enzymes) in machine dishwashing liquid composition systems, but also have the benefits of easy rinsing and making the composition milder.

[0033] The invention discloses a machine-washing dishwashing liquid composition containing sophorolipids, which has excellent detergency, is green and low-carbon, has efficient and stable enzyme activity in the composition, has good foaming properties and is easy to rinse, has extremely low cytotoxicity and other effects that are significantly better than those of the prior art solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying any creative work.

[0035] Figure 1 These are the human skin keratinocyte toxicity evaluation results of Examples 1-2 of the present invention and Comparative Examples 1-2. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0038] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0039] It should be further understood that the term "and / or" used in the present description and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0040] In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further introduced and illustrated in conjunction with specific embodiments below.

[0041] The high-acid sophorolipids described in the embodiment of the present invention are fermented and synthesized by the engineered strain in Chinese patent CN115851472A (the acid-type structure accounts for 90% by weight of the sophorolipids). The sophorolipids described in the embodiment of the present invention are fermented and synthesized by non-pathogenic Candida with C18 fatty acids and glucose as substrates, wherein the acid-type structure accounts for 50% by weight.

[0042] The raw materials except the high-acid sophorolipids involved in the present invention can be directly purchased from the market. For process parameters not specifically noted, conventional techniques can be used.

[0043] Unless otherwise stated, the raw materials and formulations of the examples and comparative examples of the present invention are all active ingredient concentrations.

[0044] According to the formulation in Table 1, the machine dishwashing liquid compositions containing sophorolipids of Examples 1-4 were prepared.

[0045] Table 1 Formula composition of Examples 1-4 (mass percentage %)

[0046]

[0047] Note: The table specifies the content of active ingredients.

[0048] The preparation method of the sophorolipid-containing machine dishwashing liquid composition of Examples 1-4 comprises the following steps:

[0049] S1. The chelating agent, alcohol solvent and suspending agent are mixed and dispersed evenly until there are no particles and clumps to obtain a mixture A; water is added to the mixture A and stirring is started;

[0050] S2. Add a strong base additive, sophorolipid or high-acid sophorolipid (or other surfactant) and stir evenly;

[0051] S3. Add a pH adjuster to adjust the pH to 7.0, then add a biological enzyme and a preservative, and stir evenly to obtain a machine-washing dishwashing liquid composition containing sophorolipids.

[0052] According to the formula composition in Table 2, the machine dishwashing liquid compositions of Comparative Examples 1-4 were prepared.

[0053] Table 2 Formula composition of comparative examples 1-4 (mass percentage %)

[0054]

[0055] Note: The table specifies the content of active ingredients.

[0056] The preparation methods of the machine dishwashing liquid compositions of Comparative Examples 1-4 are the same as that of Example 1.

[0057] Specific application effect experiment:

[0058] The machine dishwashing liquid compositions of the above examples and comparative examples were tested for relevant properties:

[0059] (I) Enzyme activity stability test: Place in a 37°C oven for 14 weeks, then test the enzyme activity.

[0060] (1) Protease activity test method

[0061] Principle of protease activity test method: Dimethyl casein (DMC) is hydrolyzed by protease into small molecular amino acids, and the released amino acid clusters form colored complexes with trinitrobenzene sulfonic acid (TNBS). The absorbance value of the solution after the reaction is tested at a wavelength of 405nm by the fully automatic biochemical analyzer Konelab, and the change in absorbance per unit time is calculated to determine the size of the enzyme activity.

[0062] Protease test conditions:

[0063] The wavelength was 405 nm, the reaction time was 200 seconds, the temperature was 50°C, and the pH was 9.0.

[0064] The calculation method of enzyme activity retention rate is: actual enzyme activity value / theoretical enzyme activity value*100%.

[0065] The protease activity stability test was performed on the machine dishwashing liquid compositions of Examples 1-2 and Comparative Examples 1-2. The test results are shown in Table 3 below:

[0066] Table 3 Protease activity stability test results of Example 1-2 and Comparative Example 1-2

[0067]

[0068] (2) Amylase activity test method

[0069] Principle of the amylase activity test method: The substrate 4,6-acetal-(G7)-p-nitrophenyl-(G1)-α-Dmaltoheptaosid (hexylene-G7pNP) is decomposed by amylase into G2pNP, G3pNP, and G4pNP, and these fragments can be completely decomposed into glucose by α-glucoside, further forming yellow p-nitrophenol. The generation rate of p-nitrophenol is detected by the fully automatic biochemical analyzer Konelab, and the absorbance at 405nm is proportional to the amylase activity in the sample.

[0070] Amylase activity test conditions:

[0071] The wavelength was 405 nm, the reaction time was 240 seconds, the measurement time was 195 seconds, the temperature was 30°C, and the pH was 7.0.

[0072] The calculation method of enzyme activity retention rate is: actual enzyme activity value / theoretical enzyme activity value*100%.

[0073] The machine dishwashing liquid compositions of Examples 3-4 and Comparative Examples 3-4 were tested for the stability of amylase activity. The test results are shown in Table 4 below:

[0074] Table 4 Amylase activity stability test results of Example 3-4 and Comparative Example 3-4

[0075]

[0076] From the test results in Tables 3 and 4, it can be seen that in the machine washing dishwashing liquid composition of the present invention, the addition of sophorolipids or high-acid sophorolipids as surfactants significantly improves the enzyme activity stability of amylase and protease. In particular, the machine washing dishwashing liquid composition group containing sophorolipids in Examples 2 and 4 has the best enzyme activity retention rate due to the addition of high-acid sophorolipids.

[0077] (II) Irritation Evaluation

[0078] The present invention uses a human skin keratinocyte (Hacat cell) exposure experiment to evaluate the irritation of a machine-washing dishwashing liquid composition containing sophorolipids. The cell exposure experiment is a cytotoxicity test, which uses human skin keratinocytes as the target organ of skin irritation. After the test substance intervenes in the cells for 24 hours, the cytotoxicity is determined by the CCK8 method, and the skin irritation of the test substance is determined by the change in cell activity. When the test cell survival rate is 80% (CV80), the corresponding test substance concentration, the higher the concentration, the lower the cytotoxicity of the test substance. The human skin keratinocyte toxicity evaluation results of Examples 1-2 of the present invention and Comparative Examples 1-2 are shown in the figure. Figure 1 shown.

[0079] Depend on Figure 1 The cytotoxicity evaluation results show that the machine-washing dishwasher composition containing sophorolipids in the embodiments of the present invention has low cytotoxicity, especially the machine-washing dishwashing liquid composition containing sophorolipids in Example 2. It can be seen that the machine-washing dishwashing liquid composition containing sophorolipids in the embodiments of the present invention is less irritating than the product in the comparative example, and is milder and safer.

[0080] (III) Foam performance test

[0081] The present invention refers to the GB / T 9985 standard to evaluate the foaming condition of the machine washing dishwashing liquid composition containing sophorolipids when used, weigh 2 grams of the machine washing dishwashing liquid composition containing sophorolipids, add 998 ml of water, stir well, and prepare a washing solution with a mass concentration of 2‰, and then drop the washing solution at a height of 50 cm to generate foam, and test the foam height at 0 min and the foam height after 10 min to characterize the foaming ability and defoaming speed of the machine washing dishwashing liquid composition containing sophorolipids. The machine washing dishwashing liquid composition containing sophorolipids of Examples 1-2 and Comparative Examples 1-2 was tested for foam performance, and the test results are shown in Table 5:

[0082] Table 5 Foam performance test results of Examples 1-2 and Comparative Examples 1-2

[0083]

[0084] From the test results in Table 5, it can be seen that the machine washing dishwashing liquid composition containing sophorolipids prepared in the embodiment of the present invention has better foaming properties than the comparative example, and has the characteristics of faster defoaming, providing good decontamination effect while being easier to rinse and less likely to leave residue, which greatly matches the use characteristics and requirements of machine washing dishwashing liquid.

[0085] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A machine dishwashing liquid composition containing sophorolipids, characterized in that Includes the following mass percentage components: Sophorolipid or high-acid sophorolipid 1-5%, biological enzyme 0.3-5%, alcohol solvent 2-15%, strong alkali auxiliary agent 5-20%, pH regulator 0.02-2%, chelating agent 10-20%.

2. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The biological enzyme is selected from one or more of protease and amylase.

3. The machine dishwashing liquid composition containing sophorolipids according to claim 2, characterized in that The mass percentage of the protease in the machine-washing dishwashing liquid composition containing sophorolipids is 0.5-3%, and the mass percentage of the amylase in the machine-washing dishwashing liquid composition containing sophorolipids is 0.3-2%.

4. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The alcohol solvent includes one or more of glycerol and propylene glycol.

5. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The strong alkali auxiliary agent is heavy soda ash.

6. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The pH regulator is citric acid.

7. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The chelating agent includes one or more of glutamic acid diacetate sodium salt, sodium citrate, and methylglycine diacetate trisodium salt.

8. The machine dishwashing liquid composition containing sophorolipids according to claim 1, characterized in that The invention also comprises a suspending agent and a preservative, wherein the suspending agent comprises one or more of xanthan gum, cross-linked polyacrylic acid salt and cross-linked polyacrylic acid ester.

9. The method for preparing a machine dishwashing liquid composition containing sophorolipids according to claim 8, characterized in that: The following steps are involved: S1. The chelating agent, alcohol solvent and suspending agent are mixed and dispersed evenly until there are no particles and clumps to obtain a mixture A; water is added to the mixture A and stirring is started; S2. Add a strong base additive, sophorolipid or high-acid sophorolipid and stir evenly; S3. Add a pH adjuster to adjust the pH to 6.8-8.0, then add a biological enzyme and a preservative, and stir evenly to obtain a machine-washing dishwashing liquid composition containing sophorolipids.

10. Use of the sophorolipid-containing machine dishwashing liquid composition according to any one of claims 1 to 8 in the preparation of a special detergent for a dishwasher.

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