Pleurotus eryngii strain with light color and high yield

Abstract

The invention provides a light-color and high-yield pleurotus eryngii strain, and belongs to the field of agricultural microorganisms. The Pleurotus eryngii strain is Pleurotus eryngii Fu Pleurotus eryngii 64, and is preserved in the China Center for Type Culture Collection on November 7, 2024, the preservation number is CCTCC NO: M 20242460, and the preservation address is No. 299 on eight road in Wuchang District, Wuhan, Hubei Province. The pileus of the 'Fu Pleurotus eryngii 64' is concave, the 'Fu Pleurotus eryngii 64' is light gray, the stipe color is pure white, and good development and application prospects are achieved.

Description

Technical Field

[0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a light-colored and high-yield Pleurotus eryngii strain. Background Art

[0002] Pleurotus eryngii is the trade name of Pleurotus eryngii, also known as dried scallop mushroom, snow mushroom, almond abalone mushroom, grassland boletus, etc. It belongs to the Basidiomycetes, Basidiomycetes, Hymecomycetes, Agaricales, Pleurotus, and Pleurotus in taxonomy. Pleurotus eryngii is rich in nutrition, with thick flesh and crispy taste. It has the taste of abalone and the flavor of dried scallop.

[0003] The King Oyster Mushroom variety 'Riyin No. 1' is currently the main variety of King Oyster Mushroom factory cultivation in my country, with a variety coverage rate of more than 85%. However, the cap and stipe of this variety are dark in color, which can easily lead consumers to mistakenly believe that it has been stored for a long time and is not fresh. The color of King Oyster Mushroom fruiting body is an important commodity trait. For the same category of King Oyster Mushroom, consumers often give priority to King Oyster Mushroom with good sensory perception. Therefore, it is necessary to select King Oyster Mushroom varieties with better colors to improve the market competitiveness of King Oyster Mushroom. Summary of the invention

[0004] The purpose of the present invention is to provide a light-colored and high-yield Pleurotus eryngii strain, which can improve the market competitiveness of Pleurotus eryngii.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] A Pleurotus eryngii strain 'Fuxingbao 64', the Pleurotus eryngii strain 'Fuxingbao 64' is classified and named Pleurotus eryngii Fuxingbao 64, and was deposited in the China Center for Type Culture Collection on November 7, 2024, with the preservation number CCTCC NO: M 20242460, and the preservation address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0007] Furthermore, the mycelium growth temperature range of Pleurotus eryngii strain Fuxingbao 64 is 10°C to 30°C, the optimum growth temperature is around 25°C, and when cultured at 30°C, the mycelium growth rate of Fuxingbao 64 is faster than that of 'Riyin 1'.

[0008] After the pilot cultivar comparison test, the fruiting body of 'Fuxingbao 64' is bowling ball-shaped, the cap is concave and light gray, the stipe is white, the yield is high, and it is resistant to storage and transportation. The average yield of 5 batches of 'Fuxingbao 64' is 622.9 grams per bag, which is 15.31% higher than the control variety 'Riyin No. 1' and 37.06% higher than the control variety 'DUS24'.

[0009] The present invention has the following advantages:

[0010] The strain Fuxingbao 64 bred by the present invention has a concave cap, light gray, a white stipe and a dense texture, and has good commercial properties. The mycelium growth temperature range of Fuxingbao 64 is 10°C to 35°C, and the optimum growth temperature is 25°C. The total average yield of 'Fuxingbao 64' is 15.31% higher than that of the main cultivated variety 'Riyin No. 1' and 37.06% higher than that of the parent strain DUS24, and has a good development and application prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 : Fruiting bodies of Pleurotus eryngii strain 'Fuxingbao 64' and its parents 'Riyin 1' and DUS24.

[0012] Figure 2 : PCR electrophoresis of specific fragments of Pleurotus eryngii strain 'Fuxingbao 64' and its parent 'Riyin 1' and parent DUS24, where M. 2000bp Marker; 1. Riyin 1; 2. Fuxingbao 64; 3. DUS24. DETAILED DESCRIPTION

[0013] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further explained below in combination with specific implementation methods. However, the following examples are merely examples of the present invention and do not represent the scope of protection of the rights limited by the present invention. The scope of protection of the present invention shall be subject to the claims.

[0014] In the following examples, the tested Pleurotus eryngii strain 'Riyin No. 1' (experimental number Pl.e0100) and strain DUS24 were both derived from the Fujian Provincial Center for the Collection and Management of Edible and Medicinal Fungi Germplasm Resources (Wei Chuanzheng, Wang Meng, Zhang Peng, Liu Fang, Yan Junjie, Xie Baogui, Deng Youjin, Xie Luyu. Identification of Pleurotus eryngii strains by MNP markers based on second-generation sequencing technology [J]. Journal of Edible Fungi, 2023, 30(01): 1-9.).

[0015] In the following examples, the PDA medium has a formula of: 200 g potato, 20 g glucose, 20 g agar, and 1000 mL water.

[0016] In the following embodiment, the culture medium formula used for the bag planting is: 42.28% sawdust, 42.28% corn cobs, 6.67% wheat bran, 3.7% corn flour, 3.7% soybean meal, 0.93% lime, 0.44% calcium bicarbonate, 67% ± 2% moisture content, and each bag weighs 1500g (465g dry material).

[0017] Example 1: Breeding of Pleurotus eryngii 'Fuxingbao 64'

[0018] The breeding of Pleurotus eryngii 'Fuxingbao 64' includes the following steps:

[0019] (1) Creation of breeding materials: The parents 'Riyin No. 1' and DUS24 were subjected to fruiting. After the fruiting bodies grew out, mature fruiting bodies were selected for collecting spores. Spore germination tests were performed separately. Finally, 50 single-spore strains of 'Riyin No. 1' and 30 single-spore strains of DUS24 were obtained.

[0020] (2) Single-spore hybridization: 26 single-spore strains of 'Riyin No. 1' and 11 single-spore strains of DUS24 with good growth potential were selected and paired in pairs, with a total of 286 paired hybrid combinations. They were cultured in a 25°C constant temperature incubator. When the hyphae covered the inclined surface of the test tube, the hyphae at the front base were taken to observe the lock-like union. The hybrids with lock-like union were identified as hybrids. After microscopic examination, 63 hybrids with lock-like union were found. Based on the growth rate and growth potential of the hyphae, 14 hybrids with abnormal growth were eliminated, and the remaining 49 were retained for subsequent screening.

[0021] (3) Screening of hybrids: The 49 hybrid strains with normal growth obtained from hybridization were screened for fruiting test at Zhangzhou Tianhua Edible Fungi Co., Ltd. The fruiting management was carried out according to the company's mushroom bag cultivation method. One hybrid strain (named Fuxingbao 64) with better quality traits (color and yield) than the control varieties 'Riyin No. 1' and DUS24 was selected for re-screening test.

[0022] (4) The hybrid strain Fuxingbao 64 was cultivated and managed in the bag cultivation mode in the re-screening experiment. The fruiting characteristics of the hybrid strain were better. The average single-bottle yield was greater than 560 grams, the cap was light in color and round, the stem was white and dense, and the commercial characteristics were good.

[0023] The hybrid strain Fuxingbao 64 was bred by hybridizing Pleurotus eryngii 'Riyin No. 1' and 'DUS24' as parents. 'Riyin No. 1' has the characteristics of high yield, firm mushroom body, and good consistency of mushroom production. It is a variety widely used in factory cultivation of Pleurotus eryngii. However, this variety has the defect of dark cap color, and some consumers mistakenly believe that it is not fresh. The other parent 'DUS24' has the characteristics of lighter cap color and good commercial traits, but lower yield. After cultivation experiments, 'Fuxingbao 64' showed high and stable yield, light and round cap color, white stipe and dense texture, and better commercial traits than 'Riyin No. 1' and 'DUS 24'( Figure 1 ).

[0024] The Pleurotus eryngii strain 'Fuxingbao 64' was classified and named Pleurotus eryngii Fuxingbao 64, and was deposited in the China Center for Type Culture Collection on November 7, 2024, with the preservation number CCTCC NO: M 20242460, and the preservation address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0025] In the early stage of breeding, the parent 'Riyin No. 1' was hybridized with DUS series strains (numbered DUS 01~DUS 32) respectively. The hybrid strain Fuxingbao 64 finally screened out had the lightest cap and stipe color and the best traits.

[0026] Example 2: Effects of different temperatures on mycelial growth of Pleurotus eryngii 'Fuxingbao 64'

[0027] The hybrid strains of Pleurotus eryngii, Fuxingbao 64 and 'Riyin 1', were inoculated on PDA culture medium and cultured at constant temperatures of 5℃, 10℃, 15℃, 20℃, 25℃, 30℃ and 35℃. As can be seen from Table 1, the mycelium growth temperature range of Fuxingbao 64 is between 10℃ and 35℃, and the optimum growth temperature is about 25℃. At 30℃ and 35℃, the mycelium growth rate of Fuxingbao 64 is faster than that of 'Riyin 1', but at other temperatures, the mycelium growth rate is slower than that of 'Riyin 1'. Fuxingbao 64 and 'Riyin 1' are both weak in low temperature resistance, and the mycelium of both did not grow at 5℃.

[0028] Table 1 Mycelium growth rate test results (unit: cm / day)

[0029]

[0030] Example 3: Batch planting of Pleurotus eryngii strain 'Fuxingbao 64'

[0031] Taking the main cultivated variety of Pleurotus eryngii 'Riyin No. 1' as the control, the 'Fuxingbao 64', 'Riyin No. 1' and another parent DUS24 strains were activated and liquid strains were made, and then production and planting were carried out. Bag planting was adopted. The planting location was Zhangzhou Tianhua Edible Fungi Co., Ltd., and the mushroom production management was carried out in the production method of Pleurotus eryngii planted by the company. Five batches were planted continuously, and 5000 bags of each variety were planted in each batch. The yield, cap diameter, cap thickness, stipe diameter, and stipe length were measured at the time of harvesting each batch. The measurement data of 'Fuxingbao 64' and the control variety 'Riyin No. 1' and the parent DUS24 were summarized and analyzed for significant differences. The results are listed in Table 2.

[0032] Table 2 Results of multiple batches of cultivation experiments

[0033]

[0034] After five batches of cultivation trials, the total average yield of 'Fuxingbao 64' was 624.2 g / bag, the average cap diameter was 61.45 mm, the thickness was 30.13 mm, the average stem diameter was 55.27 mm, and the length was 11.16 cm. The main variety 'Riyin No. 1' had a total average yield of 541.3 g / bag, the average cap diameter was 63.63 mm, the thickness was 38.52 mm, the average stem diameter was 50.57 mm, and the length was 14.68 cm. The parent strain DUS24 had a total average yield of 455.4 g / bag, the average cap diameter was 51.10 mm, the thickness was 36.05 mm, the average stem diameter was 49.76 mm, and the length was 14.06 cm. The total average yield of 'Fuxingbao 64' was 15.31% higher than that of the main cultivated variety 'Riyin No. 1' (the difference reached an extremely significant level), and 37.06% higher than that of the parent strain DUS24 (the difference reached an extremely significant level).

[0035] Example 4: DNA fingerprints of Pleurotus eryngii 'Fuxingbao 64' and its parent 'Riyin 1'

[0036] The DNA samples were sent to Beijing Novogene Bioinformatics Technology Co., Ltd. for whole genome sequencing. After obtaining the sequencing data, the whole genome sequencing data of 'Fuxingbao 64', the parent 'Riyin No. 1', and the parent DUS24 were compared pairwise using Kmer subtractive assembly technology to obtain differential fragments (SVs), and then these differential fragment sequences were used to design specific primers. The primer sequences are shown in Table 3. After the primers were synthesized, PCR verification was performed on the samples to be tested, and the Pleurotus eryngii 'Fuxingbao 64' and the parent 'Riyin No. 1' and the parent DUS24 were amplified. For specific detection methods, see (A strain of Pleurotus eryngii CN202010860991.6, announcement date 2022-03-22).

[0037] Table 3 SV primer sequences

[0038]

[0039]

[0040] The PCR amplification results of each specific fragment are shown in Figure 2As shown: P1~P5 are common fragments of 'Fuxingbao 64' and 'Riyin 1', with sizes of 1327bp, 1048bp, 1382bp, 992bp and 1296bp respectively, which are different from 'DUS24'; P6~P10 are common fragments of 'Fuxingbao 64' and 'DUS24', with sizes of 983bp, 897bp, 1252bp, 917bp and 995bp respectively, which are different from 'Riyin 1'; P11~P13 are unique fragments of 'Riyin 1' that are different from 'Fuxingbao 64' and 'DUS24', with sizes of 1005bp, 1024bp and 1372bp respectively; P14~P16 are unique fragments of 'DUS24' that are different from 'Fuxingbao 64' and 'Riyin 1', with sizes of 1203bp, 1203bp and 629bp respectively. From the results, it can be concluded that there are obvious differences in DNA fingerprints between Pleurotus eryngii 'Fuxingbao 64' and its parents 'Riyin No. 1' and DUS24, and they are determined to be different varieties.

[0041] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A light-colored and high-yield Pleurotus eryngii strain, characterized by: For Pleurotus eryngii ( Pleurotus eryngii ) Fuxingbao 64 was deposited in the China Center for Type Culture Collection on November 7, 2024, with the deposit number CCTCC NO: M20242460, and the deposit address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

2. The light-colored and high-yield Pleurotus eryngii strain according to claim 1, characterized in that: The mycelium growth temperature of the abalone 64 is in the range of 10°C to 30°C.

3. The light-colored and high-yield Pleurotus eryngii strain according to claim 1, characterized in that: The fruiting body of the abalone 64 is in the shape of a bowling ball, the cap is concave and light grey, and the stipe is white.

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