Integrated incubation and washing module for a fully automated single molecule immunoassay device

Abstract

The application discloses an integrated incubation and cleaning module of a full-automatic single-molecule immune analysis device, which comprises an outer shell, a cleaning disc and an incubation disc rotatably connected in the outer shell, the incubation disc is annular, the cleaning disc is located at the inner circumferential side of the annular incubation disc, an adjustable support structure is further arranged in the outer shell, and the annular incubation disc is rotatably connected in the outer shell through the adjustable support structure; a reaction cup internal transfer mechanism is further arranged on the outer shell, the reaction cup internal transfer mechanism is used for transferring the reaction cup between the cleaning disc and the incubation disc in the integrated incubation and cleaning module, and the reaction cup internal transfer mechanism is used for discarding the reaction cup after detection from the integrated incubation and cleaning module. When the reaction cup is transferred and discarded, the operation is carried out in the integrated incubation and cleaning module, so that the overall structure of the immune analysis device is simplified, and the overall cost of the immune analysis device is reduced.

Description

TECHNICAL FIELD

[0001] The application relates to an integrated incubation and cleaning module, in particular to an integrated incubation and cleaning module of a full-automatic single-molecule immune analysis device, and belongs to the technical field of medical examination. BACKGROUND

[0002] Since the first automatic chemical analysis instrument was manufactured, more than half a century has passed, and full-automatic immune analysis instruments have been mature in technology. The full-automatic immune analysis instruments have the following characteristics: 1, a multi-degree-of-freedom mechanical arm is adopted to coordinate the actions between modules; 2, the instrument has strong flexibility and can meet various analysis requirements; 3, the test speed is high, and the instrument can continuously operate for a long time without human intervention; 4, various technologies are combined in the detection technology, and complete automation is adopted in processing, so that the detection result is more accurate and the precision is higher. The full-automatic immune analysis instrument can realize the steps of taking a reaction cup, adding a sample, adding a reaction liquid, shaking, promoting a reaction, measuring, operation and analysis, cleaning and the like in the experimental test process, and the instrument replaces manual operation, not only saving labor cost, but more importantly, eliminating human errors and ensuring the accuracy of data. The full-automatic immune analysis instrument has the advantages of rapidness, high efficiency, high precision, repeatability and the like, and is widely applied in the fields of processing, production, testing and life assistance, and will inevitably become a trend in the medical detection field.

[0003] In the previous technology, the main process of the incubation and cleaning device in the immune analysis instrument is that the reaction cup added with a sample and a reagent is placed in an independent incubation module to be heated and incubated, and then the reaction cup is taken out by a mechanical hand and placed in another cleaning module to be magnetically separated and cleaned. Since the incubation and the cleaning are two completely independent modules, the overall volume of the immune analysis device is large, the structure is complex, and the cost is also high. Therefore, in the prior art, the incubation disc responsible for incubation and the cleaning disc responsible for cleaning are coaxially arranged and integrated into an incubation and cleaning module. In this way, the overall volume of the immune analysis device is reduced compared with the previous one.

[0004] During the working process, the position of the reaction cup needs to be exchanged between the cleaning disc and the incubation disc, and in the integrated incubation and cleaning module, the prior art adopts an external mechanical hand, that is, the external mechanical hand is used to take out the reaction cup on the cleaning disc and place it in the incubation disc or take out the reaction cup on the incubation disc and place it in the cleaning disc. In addition, when the reaction cup in the integrated incubation and cleaning module needs to be discarded after detection, the external mechanical hand is also needed to take it out and then discard it. Therefore, in the prior art, when the reaction cup is transported and discarded, the mechanical hand arranged outside the incubation and cleaning module is used to complete the transportation and discarding, so that the problem of the large overall volume of the entire immune analysis device is still caused to a certain extent.

[0005] The relevant patent documents retrieved are as follows:

[0006] I. The Chinese utility model patent with the authorization announcement number CN207636603U and the authorization announcement date of July 20, 2018 discloses an incubation and cleaning device, which comprises an incubation disc, a cleaning disc, a driving assembly and a heating assembly. The incubation disc comprises an incubation disc body for incubating samples in reaction cups. The cleaning disc is coaxial with the incubation disc and is arranged in concentric circles. The cleaning disc is used for multi-stage cleaning of magnetic microparticles in the reaction cups. The driving assembly is a coaxial driving structure comprising two driving motors for driving the incubation disc and the cleaning disc to move independently. The heating assembly is located below the incubation disc and the cleaning disc for heating the incubation disc and the cleaning disc.

[0007] II. The Chinese utility model patent with the authorization announcement number CN217688992U and the authorization announcement date of October 28, 2022 discloses a sample analysis device, which comprises: a reaction container supply unit for providing unused reaction containers; a dispensing unit for injecting reagents or / and samples into the reaction containers; an incubation disc unit for incubating liquids in the reaction containers; a cleaning disc unit for removing unbound components in the reaction system in the reaction containers; a detection unit for detecting analytes in the reaction containers; the cleaning disc unit is coaxially arranged outside or inside the incubation disc unit, and the cleaning disc unit and the incubation disc unit independently operate; a reaction container transfer mechanism for transferring the reaction containers between the reaction container supply unit, the incubation disc unit and the cleaning disc unit; the reaction container supply unit has a reaction container supply unit cup grabbing position, the incubation disc unit has an incubation disc cup grabbing position, and the cleaning disc unit has a cleaning disc cup grabbing position; the reaction container supply unit cup grabbing position, the incubation disc cup grabbing position and the cleaning disc cup grabbing position are located on the movement track of the reaction container transfer unit.

[0008] The above-mentioned patent documents disclose incubation and cleaning modules, but do not disclose technical solutions to solve the above-mentioned technical problems.

[0009] In summary, how to design an integrated incubation and cleaning module of a full-automatic single-molecule immunoassay device to minimize external operation and reduce the setting of external related components when transferring and discarding reaction cups, thereby minimizing the overall volume of the immunoassay device, simplifying the overall structure of the immunoassay device and reducing the overall cost of the immunoassay device is a technical problem that needs to be solved. SUMMARY

[0010] The technical problem solved by the present application is to provide an integrated incubation and cleaning module of a full-automatic single-molecule immunoassay device, which changes the current external operation mode, reduces the setting of external related components, thereby minimizing the overall volume of the immunoassay device, simplifying the overall structure of the immunoassay device, and reducing the overall cost of the immunoassay device.

[0011] To solve the above technical problems, the technical solution adopted by the present application is as follows: an integrated incubation and cleaning module of a full-automatic single-molecule immunoassay device, comprising a housing, a cleaning disc and an incubation disc rotatably connected inside the housing, the incubation disc being annular, the cleaning disc being located at the inner circumferential side of the annular incubation disc, and an adjustable support structure being further provided inside the housing, the annular incubation disc being rotatably connected inside the housing by the adjustable support structure; a reaction cup internal transfer mechanism is further provided on the housing, which is used to transfer the reaction cup between the cleaning disc and the incubation disc inside the integrated incubation and cleaning module and to discard the reaction cup after detection from the integrated incubation and cleaning module.

[0012] Preferably, the adjustable support structure comprises at least two fixed-position horizontal guide wheel structures and at least one position-adjustable horizontal guide wheel structure provided inside the housing, which are used to be in transmission connection with the inner circumferential side of the annular incubation disc, so that the incubation disc is rotatably connected inside the housing.

[0013] Preferably, the fixed-position horizontal guide wheel structure comprises a support column one and a horizontal guide wheel one provided on the top of the support column one, a guide groove being formed on the circumferential surface of the horizontal guide wheel one and being used to be in connection with the annular incubation disc, and the position-adjustable horizontal guide wheel structure comprises a support column two and a support column three, a swing arm being hinged on the top of the support column two, one end of the swing arm being hinged on the top of the support column two, a swing arm waist hole being formed on the other end of the swing arm, a screw one being screwed into the top of the support column three through the swing arm waist hole, so that the other end of the swing arm is locked on the top of the support column three, a horizontal guide wheel two being provided on the swing arm, and a guide groove being also formed on the circumferential surface of the horizontal guide wheel two and being used to be in connection with the annular incubation disc.

[0014] Preferably, the incubation disc comprises an upper ring body and a lower ring body, the upper ring body and the lower ring body being connected into one body, the inner circumferential side of the upper ring body being clamped into the guide grooves of the horizontal guide wheel one and the horizontal guide wheel two, so that the incubation disc is rotatably connected inside the housing.

[0015] Preferably, an outer gear is arranged on the outer circumferential surface of the upper ring body; a driving mechanism two is arranged on the outer shell body, and a transmission gear is arranged on the output shaft of the driving mechanism two, which is in transmission connection with the outer gear of the upper ring body through the transmission gear, so that the incubation disc can be driven to rotate by the driving mechanism two.

[0016] Preferably, the cleaning disc comprises an upper disc body and a lower disc body, and the upper disc body is connected with the lower disc body to form a whole; a driving mechanism one is arranged on the outer shell body, and the output shaft of the driving mechanism one is connected with the central position of the lower disc body, so that the cleaning disc can be driven to rotate under the driving of the driving mechanism one.

[0017] Preferably, a plurality of U-shaped grooves one for supporting the reaction cup are arranged on the lower disc body along the circumferential direction of the cleaning disc, and the U-shaped grooves one are arranged along the radial direction of the lower disc body; a plurality of U-shaped grooves two for supporting the reaction cup are arranged on the lower ring body of the incubation disc along the circumferential direction of the incubation disc, and the U-shaped grooves two are arranged along the radial direction of the lower ring body.

[0018] When the reaction cup is transported between the cleaning disc and the incubation disc by the reaction cup internal transport mechanism, one U-shaped groove one of the cleaning disc and one U-shaped groove two of the incubation disc are located at the same radial line.

[0019] Preferably, the reaction cup internal transport mechanism comprises a connecting seat arranged on the outer shell body, an X-axis moving device arranged on the connecting seat, and a Z-axis moving device arranged on the X-axis moving device, and a transport cylinder is arranged on the Z-axis moving device, and the X-axis moving device and the Z-axis moving device form a two-dimensional moving mechanism, and the X-axis is arranged along the radial direction of the cleaning disc and the incubation disc in the integrated incubation and cleaning module.

[0020] Preferably, the X-axis moving device comprises a motor one arranged on the connecting seat and a lead screw one rotatably connected to the connecting seat through a bearing, the output shaft of the motor one is in transmission connection with the lead screw one, a nut seat one is further slidably connected to the connecting seat, and the nut seat one is in connection with the lead screw one to form a lead screw nut mechanism one, so that the nut seat one can be driven to move along the X-axis direction under the action of the motor one; the Y-axis moving device comprises a motor two arranged on the nut seat one and a lead screw two rotatably connected to the nut seat one through a bearing, the output shaft of the motor two is in transmission connection with the lead screw two, a nut seat two is further slidably connected to the nut seat one, and the nut seat two is in connection with the lead screw two to form a lead screw nut mechanism two, so that the nut seat two can be driven to move up and down along the Y-axis direction under the action of the motor two; and the transport cylinder is connected to the nut seat two.

[0021] Preferably, the transport cylinder comprises an outer frame and a cylinder body arranged inside the outer frame, the cylinder body is a two-piece structure comprising a left half cylinder body and a right half cylinder body, a first support rod and a second support rod are arranged on the two sides of the left half cylinder body respectively, a third support rod and a fourth support rod are arranged on the two sides of the right half cylinder body respectively, a first spring is arranged between the first support rod and the third support rod, a second spring is arranged between the second support rod and the fourth support rod, the left half cylinder body and the right half cylinder body are folded to form the cylinder body through the first spring and the second spring, a first guide rod and a second guide rod are further arranged on the two sides of the left half cylinder body respectively, a third guide rod and a fourth guide rod are further arranged on the two sides of the right half cylinder body respectively, and a guide wheel is connected to each guide rod;

[0022] A first guide groove and a second guide groove are further arranged on the opposite sides of the outer frame, when the folded cylinder body is placed into the outer frame, the guide wheels on the first guide rod and the third guide rod are in contact with the first guide groove, and the guide wheels on the second guide rod and the fourth guide rod are in contact with the second guide groove, so that the cylinder body is connected to the outer frame, a first frame waist hole and a second frame waist hole are further arranged on the opposite sides of the outer frame, one end of the first support rod and the third support rod extends out of the first frame waist hole, and one end of the second support rod and the fourth support rod extends out of the second frame waist hole;

[0023] A conical opening and closing guide plate is further arranged on the bottom of the integrated incubation and cleaning module, the opening and closing guide plate is located below the transport cylinder, a roller is arranged on one end of the first support rod and the third support rod, and the opening and closing guide plate cooperates with the rollers of the transport cylinder, so that the left half cylinder body and the right half cylinder body of the transport cylinder can be separated.

[0024] The beneficial effects of the present application are that the reaction cup transport and discarding are carried out from the inside of the integrated incubation and cleaning module, the external operation mode is changed, the external related components are reduced, the overall volume of the immunoassay device is minimized, the overall structure of the immunoassay device is simplified, and the overall cost of the immunoassay device is reduced. Through the overall design of the present application, the reaction cup enters the inside of the integrated incubation and cleaning module, and does not need to repeatedly enter and exit the integrated incubation and cleaning module during the entire detection process, the entire operation process including the discarding process is completed in the integrated incubation and cleaning module, thereby realizing a brand-new internal operation mode, greatly optimizing the overall structure and operation program of the immunoassay device, and avoiding the occurrence of reaction cup pollution problems caused by external environment. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the integrated incubation and cleaning module in the embodiment of the present application.

[0026] Figure 2This is an axial cross-sectional three-dimensional structural diagram of the integrated incubation and cleaning module in an embodiment of the present invention;

[0027] Figure 3 This is a three-dimensional structural diagram of the cleaning disc in an embodiment of the present invention;

[0028] Figure 4 for Figure 3 A partial three-dimensional structural diagram of a location in the U-shaped groove;

[0029] Figure 5 This is a three-dimensional structural diagram of the incubation tray in an embodiment of the present invention;

[0030] Figure 6 This is a three-dimensional structural diagram of the integrated incubation and cleaning module after removing the cleaning tray in an embodiment of the present invention;

[0031] Figure 7 for Figure 6 A partial three-dimensional structural diagram of the part located at point two of the U-shaped groove;

[0032] Figure 8 This is a three-dimensional structural diagram of the internal transfer mechanism of the reaction vessel in an embodiment of the present invention;

[0033] Figure 9 This is a partial top view of the U-shaped groove one in the cleaning tray and the U-shaped groove two in the incubation tray in the integrated incubation and cleaning module of this invention.

[0034] Figure 10 This is a schematic diagram illustrating the principle of the adjustable support method for the incubation tray in an embodiment of the present invention;

[0035] Figure 11 for Figure 6 Enlarged structural diagram of section C;

[0036] Figure 12 This is a schematic diagram illustrating the principle and structure of the internal transport method of the reaction vessel in this embodiment of the invention. Figure 1 ;

[0037] Figure 13 This is a schematic diagram illustrating the principle and structure of the internal transport method of the reaction vessel in this embodiment of the invention. Figure 2 ;

[0038] Figure 14 This is a schematic diagram illustrating the principle and structure of the internal transport method of the reaction vessel in this embodiment of the invention. Figure 3 ;

[0039] Figure 15 This is a schematic diagram illustrating the principle and structure of the internal transport method of the reaction vessel in this embodiment of the invention. Figure 4 ;

[0040] Figure 16This is a schematic diagram illustrating the principle and structure of the internal transport method of the reaction vessel in this embodiment of the invention. Figure 5 ;

[0041] Figure 17 This is a schematic diagram of the main structure of the transfer cylinder lifting the reaction cup in an embodiment of the present invention;

[0042] Figure 18 This is a schematic diagram of the three-dimensional structure of the transfer cylinder in an embodiment of the present invention. Figure 1 ;

[0043] Figure 19 This is a schematic diagram of the three-dimensional structure of the transfer cylinder in an embodiment of the present invention. Figure 2 ;

[0044] Figure 20 This is a three-dimensional structural diagram of the inner cylinder of the transfer cylinder according to an embodiment of the present invention;

[0045] Figure 21 for Figure 2 Enlarged structural diagram of section A in the middle;

[0046] Figure 22 This is a schematic diagram illustrating the principle structure when discarding the reaction cup in an embodiment of the present invention. Figure 1 ;

[0047] Figure 23 This is a schematic diagram illustrating the principle structure when discarding the reaction cup in an embodiment of the present invention. Figure 2 ;

[0048] Figure 24 This is a schematic diagram illustrating the principle structure when discarding the reaction cup in an embodiment of the present invention. Figure 3 .

[0049] In the figure: 1. outer shell, 2. foot, 3. washing disc, 311. upper disc body, 312. lower disc body, 313. U-shaped groove one, 3131. step one, 4. incubation disc, 411. upper ring body, 4111. outer teeth, 412. lower ring body, 413. U-shaped groove two, 4131. step two, 414. ring body through hole, 5. drive mechanism one, 6. drive mechanism two, 611. transmission gear, 7. reaction cup, 711. reaction cup body, 712. flange, 811. support column one, 812. support column two, 813. support column three, 814. swing arm, 815. swing arm waist hole, 816. horizontal guide wheel two, 9. horizontal guide wheel one, 911. guide groove, 10. reaction cup internal transfer mechanism, 101. connecting seat, 11. transfer cylinder, 111. transfer cylinder inner cavity, 112. outer frame, 113. cylinder body, 1131. left half cylinder body, 1132. right half cylinder body, 12. stepper motor one, 13. lead screw one, 14. nut seat one, 15. stepper motor two, 16. lead screw two, 17. nut seat two, 18. support rod one, 19. support rod two, 20. support rod three, 21. support rod four, 22. spring one, 23. spring two, 24. guide rod one, 25. guide rod two, 26. guide rod three, 27. guide rod four, 28. guide wheel, 29. guide groove one, 30. guide groove two, 31. frame waist hole one, 32. frame waist hole two, 33. opening and closing guide plate, 34. roller, 35. cup dropping guide notch. DETAILED DESCRIPTION

[0050] The technical solutions of the present application will be further described in detail below in combination with the drawings and specific embodiments. EMBODIMENT

[0051] The present application first describes the structure of the integrated incubation and washing module.

[0052] As shown in Figure 1 and Figure 2 , an integrated incubation and washing module includes a circular outer shell 1, a plurality of feet 2 are further provided at the bottom of the outer shell 1 for the support of the entire module; a washing disc 3 and an incubation disc 4 are rotatably arranged inside the outer shell 1, the incubation disc 4 is arranged in a ring shape, the washing disc 3 is located inside the ring-shaped incubation disc 4, and the washing disc 3 and the incubation disc 4 are arranged on the same central axis; a drive mechanism one 5 and a drive mechanism two 6, the drive mechanism one 5 is a washing disc driving motor, which is arranged on the inner bottom surface of the outer shell 1, and its output shaft is connected with the washing disc 3 for driving the rotation of the washing disc, the drive mechanism two 6 is an incubation disc driving motor, which is arranged on the outer shell 1, and a transmission gear 611 is arranged on its output shaft, which is in tooth transmission connection with the incubation disc 4 through the transmission gear 611 for driving the rotation of the incubation disc 4, and the specific tooth transmission structure will be described later.

[0053] The structure of the washing disc 3 is described as follows: as shown in Figure 3 the washing disc 3 comprises an upper disc body 311 and a lower disc body 312, and the upper disc body 311 is connected with the lower disc body 312 into an integrated body by a connecting member such as a screw. The output shaft of the washing disc driving motor is connected with the central position of the lower disc body 312, so that the washing disc 3 can be driven to rotate under the driving of the washing disc driving motor. A plurality of U-shaped grooves 313 for supporting the reaction cup are arranged on the lower disc body 312 along the circumferential direction of the washing disc, and the U-shaped grooves 313 are arranged towards the radial direction of the lower disc body 312. As shown in Figure 4 the reaction cup 7 comprises a reaction cup body 711 and a flange 712 arranged on the reaction cup body 711, and the flange 712 is close to the opening position of the reaction cup body 711. When the reaction cup 7 is placed into the U-shaped groove 313, the flange 712 of the reaction cup 7 is placed on the groove bottom of the U-shaped groove 313, so as to support the reaction cup. In the embodiment, the groove bottom of the U-shaped groove 313 is an arc-shaped groove bottom, and a step portion 3131 is arranged on the lower disc body 312 and located at the groove bottom of the U-shaped groove 313, and the arc degree of the step portion 3131 matches the arc degree of the flange 712 of the reaction cup 7. When the reaction cup 7 is placed into the U-shaped groove 313, the flange 712 of the reaction cup 7 is placed on the step portion 3131 of the groove bottom of the U-shaped groove 313, so as to support the reaction cup. The step portion 3131 can limit the flange 712 of the reaction cup 7, so as to position the position of the reaction cup 7 on the washing disc.

[0054] The structure of the incubation disc 4 is described as follows: as shown in Figure 5 to Figure 7 the incubation disc 4 comprises an upper ring body 411 and a lower ring body 412, and the upper ring body 411 is connected with the lower ring body 412 into an integrated body by a connecting member such as a screw.

[0055] The outer teeth 4111 are arranged on the outer circumferential surface of the upper ring body 411, and the outer teeth 4111 are arranged in a whole circle along the circumferential direction of the upper ring body 411. The transmission gear 611 on the output shaft of the driving mechanism two 6 is in meshing transmission connection with the outer teeth 4111 of the upper ring body 411, so that the incubation disc 4 can be driven to rotate by the driving mechanism two.

[0056] The structure of the incubation disc for supporting the reaction cup is the same as the structure of the washing disc for supporting the reaction cup, as shown in Figure 5 and Figure 7As shown, a plurality of U-shaped grooves two 413 for supporting the reaction cups are arranged on the lower ring body 412 of the incubation tray in the circumferential direction of the incubation tray, and the U-shaped grooves two 413 are arranged towards the radial direction of the lower ring body 412. When the incubation tray and the cleaning tray are assembled, the grooves of the U-shaped grooves two 413 and the grooves of the U-shaped grooves one 313 are arranged opposite to each other, that is, the direction of the grooves of the U-shaped grooves two 413 is opposite to the direction of the grooves of the U-shaped grooves one 313, and through the rotation of the incubation tray and the cleaning tray, one of the U-shaped grooves two 413 and one of the U-shaped grooves one 313 can be arranged on the same radial line.

[0057] When the reaction cup 7 is placed into the U-shaped groove two 413, the flange 712 of the reaction cup 7 is placed on the groove bottom of the U-shaped groove two 413, so as to support the reaction cup. In the embodiment, the groove bottom of the U-shaped groove two 413 is an arc-shaped groove bottom, and a step portion two 4131 is arranged on the lower ring body 412 and located at the groove bottom of the U-shaped groove two 413, and the arc of the step portion two 4131 matches the arc of the flange 712 of the reaction cup 7, when the reaction cup 7 is placed into the U-shaped groove two 413, the flange 712 of the reaction cup 7 is placed on the step portion two 4131 of the groove bottom of the U-shaped groove two 413, so as to support the reaction cup. By arranging the step portion two 4131, the flange 712 of the reaction cup 7 can be limited, so as to position the position of the reaction cup 7 on the incubation tray.

[0058] In addition, it should be noted that before incubation and cleaning, in order to place the reaction cup into the integrated incubation and cleaning module, a ring body through hole 414 is arranged on the upper ring body 411 and located at the position corresponding to each U-shaped groove two 413, when the reaction cup is placed into the U-shaped groove two 413 through the ring body through hole 414 before incubation and cleaning, the reaction cup is placed into the incubation tray, so that the reaction cup enters the integrated incubation and cleaning module.

[0059] As shown in the drawings, Figure 6 The integrated incubation and cleaning module in the embodiment further comprises a reaction cup internal transfer mechanism 10 arranged at the bottom of the outer shell 1, and the reaction cup is transferred back and forth between the cleaning tray 3 and the incubation tray 4 through the reaction cup internal transfer mechanism 10. As shown in the drawings, Figure 8As shown, the internal transfer mechanism 10 of the reaction cup includes a connecting seat 101 connected to the bottom of the outer shell 1, an X-axis moving device disposed on the connecting seat 101, and a Z-axis moving device disposed on the X-axis moving device. A transfer cylinder 11 is disposed on the Z-axis moving device. The X-axis moving device and the Z-axis moving device constitute a two-dimensional moving mechanism. The X-axis is arranged radially along the cleaning plate 3 and the incubation plate 4, and the Z-axis is perpendicular to the X-axis direction. Through the action of the two-dimensional moving mechanism, the transfer cylinder 11 is driven to move in the two-dimensional direction, and finally the reaction cup is transferred back and forth between the cleaning plate 3 and the incubation plate 4 by the transfer cylinder 11. In this embodiment, the X-axis moving device includes a stepper motor 12 mounted on the connecting seat 101 and a lead screw 13 rotatably connected to the connecting seat 101 via bearings. The output shaft of the stepper motor 12 is connected to the lead screw 13 for transmission. A nut seat 14 is also slidably connected to the connecting seat 101. The nut seat 14 and the lead screw 13 are connected to form a lead screw and nut mechanism, so that the nut seat 14 can be driven to move back and forth along the X-axis direction when the stepper motor 12 is in motion. The Y-axis moving device includes a stepper motor 15 mounted on the nut seat 14 and a lead screw 16 rotatably connected to the nut seat 14 via bearings. The output shaft of the stepper motor 15 is connected to the lead screw 16 for transmission. A nut seat 17 is also slidably connected to the nut seat 14, and the nut seat 17 and the lead screw 16 are connected to form a lead screw-nut mechanism. Thus, under the action of the stepper motor 15, the nut seat 17 can move up and down along the Y-axis. The transfer cylinder 11 is connected to the nut seat 17. It should be noted that the X-axis and Y-axis moving devices can also adopt other structures, as long as they can achieve two-dimensional movement in the X and Y axes.

[0060] like Figure 9 As shown, the transfer method used in this embodiment utilizes the transfer cylinder 11 of the transfer mechanism 10 inside the reaction cup to drive the reaction cup 7 along the radial direction (i.e., the X-axis direction) of the cleaning plate 3 and the incubation plate 4, moving it from the U-shaped groove 313 on the cleaning plate 3 to the U-shaped groove 413 on the incubation plate 4, or from the U-shaped groove 413 on the incubation plate 4 to the U-shaped groove 313 on the cleaning plate 3, thereby realizing the transfer of the reaction cup between the cleaning plate 3 and the incubation plate 4.

[0061] from Figure 9As can be seen, in order to adapt to the action of the reaction cup internal transfer mechanism, the reaction cup internal transfer mechanism moves along the radial direction (i.e. the X-axis direction) of the washing disc 3 and the incubation disc 4. Therefore, it is required to ensure that, after the incubation disc 4 is installed, one of the U-shaped grooves two 413 on the incubation disc 4 and one of the U-shaped grooves one 313 on the washing disc 3 can be located on the same radial line B through the rotation of the incubation disc and the washing disc, so as to ensure that the reaction cup internal transfer mechanism drives the reaction cup 7 to move back and forth between the U-shaped groove one 313 on the washing disc 3 and the U-shaped groove two 413 on the incubation disc 4, and complete the transfer work. However, in reality, after the intermediate washing disc 3 is installed, due to other reasons such as manufacturing and processing of the incubation disc 4, the position of the installed incubation disc 4 cannot meet the above requirements, and therefore needs to be adjusted.

[0062] As shown in Figure 6 and Figure 7 , the incubation disc 4 in the embodiment is arranged in a ring shape and is supported and connected to the inner bottom of the outer housing 1 by three or more horizontal guide wheels 9. Therefore, as shown in Figure 9 and Figure 10 , the ring-shaped incubation disc 4 can actually be regarded as a circle, and each horizontal guide wheel 9 can be regarded as a point C. Since three points C can determine the position of a circle, in order to adjust the position of the incubation disc 4, the applicant designs at least two horizontal guide wheels 9 in the incubation disc 4 as fixed-position horizontal guide wheel structures (i.e. points C1) to keep their positions unchanged, and at least one horizontal guide wheel 9 as a position-adjustable horizontal guide wheel structure (i.e. point C2). After the washing disc 3 is installed, the position of the ring-shaped incubation disc 4 is adjusted by adjusting the position of the position-adjustable horizontal guide wheel structure (i.e. point C2), so as to ensure that, during the work process, through the rotation of the incubation disc and the washing disc, one of the U-shaped grooves two 413 on the incubation disc 4 and one of the U-shaped grooves one 313 on the washing disc 3 can be located on the same radial line B, so as to adapt to the action of the reaction cup internal transfer mechanism. Therefore, in the embodiment, there is at least one position-adjustable horizontal guide wheel structure (i.e. point C2) and at least two fixed-position horizontal guide wheel structures (i.e. points C1).

[0063] As shown in Figure 7 , the fixed-position horizontal guide wheel structure includes a support column one 811 arranged on the inner bottom of the outer housing 1 and a horizontal guide wheel one 9 arranged on the top of the support column one 811. A guide groove 911 is opened on the peripheral surface of the horizontal guide wheel one 9, and the guide groove 911 is arranged in a whole circle along the peripheral direction of the horizontal guide wheel one 9. The inner peripheral part of the upper ring body 411 is clamped into the guide groove 911 of the horizontal guide wheel one 9 to be in transmission connection with the guide groove 911, so that the incubation disc 4 is arranged to rotate in the inner part of the outer housing 1.

[0064] As shown in Figure 11 The position-adjustable horizontal guide wheel structure includes support column two 812 and support column three 813 arranged on the inner bottom of the outer shell 1, a swing arm 814 is hinged on the top of the support column two 812, one end of the swing arm 814 is hinged on the top of the support column two 812, a swing arm waist hole 815 is opened on the other end of the swing arm 814, a screw one (not shown in the figure) is screwed into the top of the support column three 813 through the swing arm waist hole 815, so as to lock the other end of the swing arm 814 on the top of the support column three 813, and a horizontal guide wheel two 816 is arranged on the swing arm 814. A guide groove is also opened on the peripheral surface of the horizontal guide wheel two 816, and the guide groove is arranged in a whole circle along the circumference of the horizontal guide wheel two 816. By clamping the inner circumferential part of the upper ring body 411 into the guide groove of the horizontal guide wheel two 816 and cooperating with the guide groove, the incubation tray 4 is arranged to rotate inside the outer shell 1.

[0065] The specific steps of the adjustable support method of the incubation tray in the embodiment are as follows: as shown in Figure 7 and Figure 11 Clamp the inner circumferential part of the upper ring body 411 of the incubation tray into the guide groove 911 of the horizontal guide wheel one 9 of the at least two position-fixed horizontal guide wheel structures and the guide groove of the horizontal guide wheel two 816 of the at least one position-adjustable horizontal guide wheel structure, adjust the position of the swing arm 814 of the at least one position-adjustable horizontal guide wheel structure, so that one of the U-shaped grooves two 413 on the incubation tray 4 and one of the U-shaped grooves one 313 on the cleaning tray 3 can be in the same radial line B, then lock the screw one, lock the swing arm 814, so that the incubation tray 4 is arranged to rotate inside the outer shell 1.

[0066] In the embodiment, one of the three connection points is set as a position-adjustable connection point, and the position of the annular incubation tray is determined by the three adjusted connection points, so that after the installation of the incubation tray in the integrated incubation and cleaning module is completed, the internal transfer mechanism of the reaction cup can be adapted, the internal transfer operation of the reaction cup is realized, the existing mode of transferring the reaction cup from the outside is changed, the setting of the external related components is reduced, the overall volume of the immune analysis device is reduced, the overall structure of the immune analysis device is simplified, and the overall cost of the immune analysis device is reduced.

[0067] The specific transfer operation of the internal transfer mechanism of the reaction cup is described below:

[0068] As shown in Figure 9As shown, when the reaction cup is transferred from the incubation tray 4 to the washing tray 3, first, the incubation tray 4 and the washing tray 3 are controlled to rotate so that the notch of one U-shaped groove two 413 on the incubation tray 4 and the notch of one U-shaped groove one 313 on the washing tray 3 are aligned along the radial direction of the incubation tray 4 and the washing tray 3, and then the specific operation steps are as follows:

[0069] 1), as shown in Figure 12 and Figure 13 , the Y-axis moving device is controlled to act so that the transfer cylinder 11 moves upward, so that the transfer cylinder 11 contacts the reaction cup 7 located in the U-shaped groove two 413 and in the process of continuous upward movement of the transfer cylinder 11, the reaction cup 7 is lifted from the U-shaped groove two 413, so that the flange 712 of the reaction cup leaves a vertical distance H1 from the notch plane of the U-shaped groove two 413, to avoid the reaction cup being hindered when moving radially;

[0070] 2), as shown in Figure 14 and Figure 15 , the X-axis moving device is controlled to act so that the transfer cylinder 11 moves along the radial direction towards the washing tray 3, driving the reaction cup 7 to move out of the notch of the U-shaped groove two 413 and then move into the notch of the U-shaped groove one 313, until it moves to a position above the bottom of the notch of the U-shaped groove one 313, at this time, the flange 712 of the reaction cup leaves a vertical distance H2 from the notch plane of the U-shaped groove one 313;

[0071] 3), as shown in Figure 16 , the Y-axis moving device is controlled to act so that the transfer cylinder 11 moves downward, so that the flange 712 of the reaction cup is placed into the U-shaped groove one 313, thereby placing the reaction cup 7 into the washing tray 3; when the transfer cylinder 11 is moved to the position, the transfer cylinder 11 is separated from the reaction cup 7, so that it does not hinder the rotation of the tray body in the subsequent detection process.

[0072] Conversely, when the reaction cup is transferred from the washing tray 3 to the incubation tray 4, the specific operation steps are just the opposite of the above, that is:

[0073] First, the incubation tray 4 and the washing tray 3 are controlled to rotate so that the notch of one U-shaped groove two 413 on the incubation tray 4 and the notch of one U-shaped groove one 313 on the washing tray 3 are aligned along the radial direction of the incubation tray 4 and the washing tray 3, and then the specific operation steps are as follows:

[0074] S1, the Y-axis moving device is controlled to act so that the transfer cylinder 11 moves upward, so that the transfer cylinder 11 contacts the reaction cup 7 located in the U-shaped groove one 313 and in the process of continuous upward movement of the transfer cylinder 11, the reaction cup 7 is lifted from the U-shaped groove one 313, so that the flange 712 of the reaction cup leaves a vertical distance H2 from the notch plane of the U-shaped groove one 313, to avoid the reaction cup being hindered when moving radially;

[0075] S2, control the X-axis moving device to move so that the transfer cylinder 11 moves along the radial direction to the incubation tray 4, drives the reaction cup 7 to move out of the slot of the U-shaped groove one 313, and then moves into the slot of the U-shaped groove two 413, until it moves to the position above the bottom of the U-shaped groove two 413, at this time, the flange 712 of the reaction cup leaves a vertical distance H1 with the slot plane of the U-shaped groove two 413;

[0076] S3, control the Y-axis moving device to move so that the transfer cylinder 11 moves downward, so that the flange 712 of the reaction cup is placed in the U-shaped groove two 413, thereby putting the reaction cup 7 into the incubation tray 4; when the transfer cylinder 11 is moved to the position, the transfer cylinder 11 is separated from the reaction cup 7, so that it does not hinder the rotation of the tray in the subsequent detection process.

[0077] Through the above specific operation steps, the transfer operation of the reaction cup in the integrated incubation and cleaning module is realized without the help of an external mechanical hand. Under the premise of ensuring the normal transfer of the reaction cup, the existing mode of transferring the reaction cup from the outside is changed, the setting of external related components is reduced, thereby reducing the overall volume of the immune analysis device, simplifying the overall structure of the immune analysis device, and reducing the overall cost of the immune analysis device.

[0078] As shown in Figure 4 and Figure 17 , the cup body 711 of the reaction cup is a conical cylinder, the large end is located at the upper position (i.e. close to the flange 712 side), and the lower end is located at the lower position (i.e. away from the flange 712 side, correspondingly, the transfer cylinder inner cavity 111 of the transfer cylinder 11 is also designed as a conical shape with a large upper end and a small lower end, as shown in Figure 12 When the reaction cup is lifted by the transfer cylinder 11, the taper surface of the transfer cylinder inner cavity 111 is matched and contacted with the taper surface of the reaction cup cup body 711, thereby lifting the reaction cup. When the taper of the taper surface of the transfer cylinder inner cavity 111 is designed to be different from the taper of the taper surface of the reaction cup cup body 711, the position where the taper surface of the transfer cylinder inner cavity 111 is matched and contacted with the taper surface of the reaction cup cup body 711 is set as the contact part S, and the position of the contact part S is adjusted up and down by adjusting the taper of the taper surface of the transfer cylinder inner cavity 111 and the taper of the taper surface of the reaction cup cup body 711. When the position of the contact part S is different, the stroke and other parameters of the upward movement of the transfer cylinder will actually be different, thereby adapting to various working conditions.

[0079] When the detection is completed, the reaction cup needs to be discarded, and the existing technology also uses an external mechanical hand to take out the reaction cup for discarding. Therefore, it must rely on the external mechanical hand to complete the operation, which leads to excessive setting of external related components and increases the overall volume of the immune analysis device. Therefore, the applicant further improves the internal transfer mechanism of the reaction cup and related positions, which are described as follows:

[0080] As shown in Figure 18 to Figure 20 the transport cylinder 11 includes an outer frame 112 and a cylinder body 113 arranged inside the outer frame 112, the cylinder body 113 is a two-half structure, including a left half cylinder 1131 and a right half cylinder 1132, a first support rod 18 and a second support rod 19 are arranged on the two sides of the left half cylinder 1131 respectively, a third support rod 20 and a fourth support rod 21 are arranged on the two sides of the right half cylinder 1132 respectively, a first spring 22 is arranged between the first support rod 18 and the third support rod 20, and a second spring 23 is arranged between the second support rod 19 and the fourth support rod 21, both the first spring 22 and the second spring 23 are tension springs, the left half cylinder 1131 and the right half cylinder 1132 are folded together to form the cylinder body 113 by the first spring 22 and the second spring 23, a first guide rod 24 and a second guide rod 25 are arranged on the two sides of the left half cylinder 1131 respectively, a third guide rod 26 and a fourth guide rod 27 are arranged on the two sides of the right half cylinder 1132 respectively, and a guide wheel 28 is connected to each guide rod. A guide groove 29 and a guide groove 30 are respectively opened on the opposite sides of the outer frame 112, when the folded cylinder body 113 is placed into the outer frame 112, the guide wheels on the first guide rod 24 and the third guide rod 26 are in contact with the guide groove 29, and the guide wheels on the second guide rod 25 and the fourth guide rod 27 are in contact with the guide groove 30, so as to connect the cylinder body 113 to the outer frame 112. In this way, when the cylinder body 113 is separated or folded, the guide wheels on the left half cylinder 1131 move along the guide groove 29 and the guide groove 30, and the guide wheels on the right half cylinder 1132 move along the guide groove 29 and the guide groove 30, so that the left half cylinder 1131 and the right half cylinder 1132 can be guided during the separation or folding movement, ensuring stability. In addition, a frame waist hole 31 and a frame waist hole 32 are respectively opened on the opposite sides of the outer frame 112, one end of the first support rod 18 and the third support rod 20 extends out of the frame waist hole 31, and one end of the second support rod 19 and the fourth support rod 21 extends out of the frame waist hole 32, so as to avoid the support rods and reduce the overall volume of the transport cylinder.

[0081] As shown in Figure 21 a conical opening and closing guide plate 33 is further arranged on the inner bottom surface of the outer shell 1 at the transport mechanism 10 inside the reaction cup, the opening and closing guide plate 33 is located below the transport cylinder 11, a roller 34 is arranged on one end of the first support rod 18 and the third support rod 20, and the opening and closing guide plate 33 cooperates with the roller 34 of the transport cylinder 11, so that the left half cylinder 1131 and the right half cylinder 1132 of the transport cylinder can be separated.

[0082] When detection is performed, the left half-cylinder body 1131 and the right half-cylinder body 1132 of the transfer cylinder are folded to form the cylinder body 113, so that the transfer cylinder inner cavity 111 is used to drive the reaction cup 7 to move; when the detection is completed, the left half-cylinder body 1131 and the right half-cylinder body 1132 of the transfer cylinder are separated by cooperating with the opening and closing guide plate of the cone, so that the reaction cup falls out of the left half-cylinder body 1131 and the right half-cylinder body 1132, that is, when the reaction cup needs to be discarded, the transfer cylinder 11 is first controlled to move to the position between the washing disc 3 and the incubation disc 4, and then the specific operation steps are as follows:

[0083] A1: As shown in Figure 22 to Figure 24 , the Y-axis moving device is controlled to act, so that the transfer cylinder 11 moves downward, and in the process of moving downward, the rollers 34 on the support rod one 18 on the left half-cylinder body 1131 and the rollers 34 on the support rod three 20 on the right half-cylinder body 1132 are respectively in contact with the two side tapered surfaces of the opening and closing guide plate of the cone and are rolled, so that the left half-cylinder body 1131 and the right half-cylinder body 1132 are relatively moved away and separated, at this time, the spring one 22 and the spring two 23 are in the stretched state, and when the separation is completed, the reaction cup 7 in the transfer cylinder falls out;

[0084] A2: The Y-axis moving device is controlled to act, so that the transfer cylinder 11 moves upward, and in the process of moving upward, the rollers 34 on the support rod one 18 on the left half-cylinder body 1131 and the rollers 34 on the support rod three 20 on the right half-cylinder body 1132 are respectively separated from the opening and closing guide plate of the cone, at this time, under the restoring force of the spring one 22 and the spring two 23, the left half-cylinder body 1131 and the right half-cylinder body 1132 are relatively moved close to each other and are folded together again.

[0085] Through the above steps, the embodiment can automatically discard the reaction cup after the detection is completed inside the integrated incubation and cleaning module, without the need for an external mechanical hand to take out the reaction cup and then discard it, further reducing the setting of external related components, thereby further reducing the overall volume of the immune analysis device, simplifying the overall structure of the immune analysis device, and reducing the overall cost of the immune analysis device.

[0086] In addition, it should be noted that through the overall design of the embodiment, the reaction cup enters the integrated incubation and cleaning module, and does not need to repeatedly enter and exit the integrated incubation and cleaning module during the entire detection process. The entire operation process including the discarding process is completed inside the integrated incubation and cleaning module, thereby realizing a brand-new internal operation mode, greatly optimizing the overall structure and operation program of the immune analysis device and avoiding the occurrence of reaction cup pollution problems caused by external environment.

[0087] AsFigure 21 As shown, a cup-dropping guide notch 35 is also arranged on the outer housing 1 at the position of the opening and closing guide plate 33, and the cup-dropping guide notch 35 is also located below the transfer cylinder 11. When the reaction cup 7 falls from the transfer cylinder 11, the reaction cup 7 falls into the cup-dropping guide notch 35 and is guided out of the integrated incubation and cleaning module through the cup-dropping guide notch 35. In this way, the discarded reaction cup can be guided out.

[0088] In summary, according to the present application, the reaction cup transfer and the reaction cup discarding are performed from inside the integrated incubation and cleaning module, which changes the current external operation mode, reduces the setting of external related components, and thus minimizes the overall volume of the immunoassay device, simplifies the overall structure of the immunoassay device, and reduces the overall cost of the immunoassay device. Through the overall design of the present application, the reaction cup enters the integrated incubation and cleaning module and does not need to repeatedly enter and exit the integrated incubation and cleaning module during the entire detection process. The entire operation process, including the discarding process, is completed inside the integrated incubation and cleaning module, thereby realizing a brand-new internal operation mode, greatly optimizing the overall structure and operation procedure of the immunoassay device, and avoiding the occurrence of the problem of reaction cup pollution caused by the external environment.

[0089] The above embodiments are only used to illustrate the present application, but not to limit the present application. Those skilled in the art can make various changes or modifications without departing from the spirit and scope of the present application. Therefore, all equivalent technical solutions should belong to the protection scope of the present application, and the protection scope of the present application should be defined by the claims.

Claims

1. An integrated incubation and washing module for a fully automated single molecule immunoassay device, comprising a housing, and a washing disc and an incubation disc rotatably connected inside the housing, characterized in that: The incubation disc is annular, the washing disc is located at the inner circumferential side of the annular incubation disc, an adjustable support structure is further arranged inside the outer shell, and the annular incubation disc is rotationally connected inside the outer shell through the adjustable support structure; a reaction cup internal transfer mechanism is further arranged on the outer shell, the reaction cup internal transfer mechanism is used for transferring the reaction cup between the washing disc and the incubation disc inside the integrated incubation and washing module, and the reaction cup internal transfer mechanism is used for discarding the reaction cup after detection from the integrated incubation and washing module; The adjustable support structure comprises at least two fixed-position horizontal guide wheel structures and at least one position-adjustable horizontal guide wheel structure arranged inside the outer shell, the at least two fixed-position horizontal guide wheel structures and the at least one position-adjustable horizontal guide wheel structure are used for transmission connection with the inner circumferential side of the annular incubation disc, so that the incubation disc is rotationally connected inside the outer shell; The fixed-position horizontal guide wheel structure comprises a support column one and a horizontal guide wheel one arranged on the top of the support column one, guide grooves for matching connection with the annular incubation disc are formed in the circumferential surface of the horizontal guide wheel one, the position-adjustable horizontal guide wheel structure comprises a support column two and a support column three, a swing arm is hinged to the top of the support column two, one end of the swing arm is hinged to the top of the support column two, a swing arm waist hole is formed in the other end of the swing arm, a screw one is screwed into the top of the support column three through the swing arm waist hole, so that the other end of the swing arm is locked on the top of the support column three, a horizontal guide wheel two is arranged on the swing arm, and guide grooves for matching connection with the annular incubation disc are also formed in the circumferential surface of the horizontal guide wheel two; The incubation disc comprises an upper ring body and a lower ring body, and the upper ring body and the lower ring body are connected into one body; the inner circumferential side of the upper ring body is clamped into the guide grooves of the horizontal guide wheel one and the horizontal guide wheel two, so that the incubation disc is rotationally connected inside the outer shell; The washing disc comprises an upper disc body and a lower disc body, and the upper disc body and the lower disc body are connected into one body; a driving mechanism one is arranged on the outer shell, and the output shaft of the driving mechanism one is matched with the central position of the lower disc body, so that the washing disc can be driven to rotate under the driving of the driving mechanism one; A plurality of U-shaped grooves one for supporting the reaction cup are arranged on the lower disc body in the circumferential direction of the washing disc, and the U-shaped grooves one are arranged in the radial direction of the lower disc body; a plurality of U-shaped grooves two for supporting the reaction cup are arranged on the lower ring body of the incubation disc in the circumferential direction of the incubation disc, and the U-shaped grooves two are arranged in the radial direction of the lower ring body; When the reaction cup is transferred between the washing disc and the incubation disc by the reaction cup internal transfer mechanism, one U-shaped groove one of the washing disc and one U-shaped groove two of the incubation disc are located at the same radial line position; The reaction cup internal transport mechanism comprises a connecting seat arranged on the outer shell, an X-axis moving device arranged on the connecting seat, and a Z-axis moving device arranged on the X-axis moving device, and a transport cylinder is arranged on the Z-axis moving device, and the X-axis moving device and the Z-axis moving device constitute a two-dimensional moving mechanism, and the X-axis is arranged along the radial direction of the washing disc and the incubation disc in the integrated incubation and cleaning module.

2. The integrated incubation and wash module of claim 1, wherein: An outer gear is arranged on the outer circumferential surface of the upper ring body; a driving mechanism two is arranged on the outer shell, and a transmission gear is arranged on the output shaft of the driving mechanism two, and the transmission gear is in transmission connection with the outer gear of the upper ring body in a meshing mode, so that the incubation disc can be driven to rotate by the driving mechanism two.

3. The integrated incubation and wash module of claim 1, wherein: The X-axis moving device comprises a motor one arranged on the connecting seat and a lead screw one rotatably connected to the connecting seat through a bearing, the output shaft of the motor one is in transmission connection with the lead screw one in a matched mode, a nut seat one is further slidably connected to the connecting seat, the nut seat one is in matched connection with the lead screw one to form a lead screw nut mechanism one, so that the nut seat one can be driven to move along the X-axis direction under the action of the motor one; the Z-axis moving device comprises a motor two arranged on the nut seat one and a lead screw two rotatably connected to the nut seat one through a bearing, the output shaft of the motor two is in transmission connection with the lead screw two in a matched mode, a nut seat two is further slidably connected to the nut seat one, the nut seat two is in matched connection with the lead screw two to form a lead screw nut mechanism two, so that the nut seat two can be driven to move up and down along the Z-axis direction under the action of the motor two; and the transport cylinder is connected to the nut seat two.

4. The integrated incubation and wash module of claim 3, wherein: The transport cylinder comprises an outer frame body and a cylinder body arranged in the outer frame body, the cylinder body is a two-half structure comprising a left half cylinder body and a right half cylinder body, a first supporting rod and a second supporting rod are respectively arranged on the two sides of the left half cylinder body, a third supporting rod and a fourth supporting rod are respectively arranged on the two sides of the right half cylinder body, a first spring is arranged between the first supporting rod and the third supporting rod, a second spring is arranged between the second supporting rod and the fourth supporting rod, the left half cylinder body and the right half cylinder body are folded to form the cylinder body through the first spring and the second spring, a first guide rod and a second guide rod are respectively arranged on the two sides of the left half cylinder body, a third guide rod and a fourth guide rod are respectively arranged on the two sides of the right half cylinder body, and a guide wheel is connected to each guide rod. Guide grooves one and two are respectively formed in the opposite two side surfaces of the outer frame body, when the folded cylinder body is placed into the outer frame body, the guide wheels on the first guide rod and the third guide rod are in matched contact with the guide groove one, and the guide wheels on the second guide rod and the fourth guide rod are in matched contact with the guide groove two, so that the cylinder body is connected to the outer frame body; frame waist holes one and two are respectively formed in the opposite two side surfaces of the outer frame body, one end of the first supporting rod and the third supporting rod extends out of the frame waist hole one, and one end of the second supporting rod and the fourth supporting rod extends out of the frame waist hole two. The bottom of the integrated incubation and cleaning module is also provided with a conical opening and closing guide plate, which is located below the transfer cylinder. Rollers are arranged at one end of the first and third supporting rods. The opening and closing guide plate cooperates with the rollers of the transfer cylinder, so that the left and right half cylinder bodies of the transfer cylinder can be separated.

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