A method for improving plant drought tolerance, sericin liquid and application thereof

Abstract

The present invention relates to the field of plant cultivation technology, and specifically discloses a method for improving plant drought resistance, a sericin solution and an application thereof, comprising the following steps: preparing a sericin solution with a concentration of 0.1 g / L to 0.5 g / L, and then spraying it on the leaves of tomato plants. The method for improving tomato plant drought resistance provided by the present invention significantly improves the survival rate, biomass and water content of tomato plants under drought stress environment.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, and specifically discloses a method for improving drought resistance of plants and application of sericin solution. Background Art

[0002] Continuous high temperatures and reduced rainfall have caused varying degrees of drought stress on plants, seriously affecting crop growth and agricultural production. There are many causes of drought stress: high temperature, strong light, and low atmospheric humidity may lead to strong transpiration of plants, causing a large amount of water loss in plants; lack of available water in the soil makes it difficult for plants to absorb water, resulting in water deficit; factors such as low soil temperature, lack of oxygen, high ion concentration, or accumulation of toxic substances hinder the physiological activities of plant roots, and they cannot absorb water normally, causing physiological drought.

[0003] Drought stress is mainly manifested as a decrease in water content in plants, resulting in water deficit, which harms plant growth, including hindering plant cell division, inhibiting plant cell elongation, reducing plant photosynthesis, blocking protein synthesis, and causing metabolic disorders, leading to slow plant growth, developmental disorders, aging and shedding of plant organs, and even withering and death of the entire plant. How to improve the tolerance of plants to drought adversity is an urgent problem to be solved. Summary of the invention

[0004] In order to improve the tolerance of plants to drought stress, the present invention provides a method for improving plant drought tolerance and the application of sericin solution. The method for improving drought tolerance of tomato plants provided by the present invention significantly improves the survival rate, biomass and water content of tomato plants under drought stress environment.

[0005] The present invention provides a method for improving plant drought tolerance, comprising the following steps:

[0006] Preparation of sericin solution: crush the silkworm cocoons and boil them in distilled water for 10 to 15 minutes to obtain a sericin solution, which is then diluted with distilled water to a concentration of 0.1 g / L to 0.5 g / L to obtain a sericin solution;

[0007] When the tomato plants grow to five leaves and one bud, spray the front of the leaves with sericin solution at a rate of 10 mL / plant to 15 mL / plant.

[0008] Silk protein fiber is mainly composed of outer sericin wrapped in inner fibroin, of which the outer sericin component accounts for about 20%. As a natural protein material, sericin has good moisturizing, anti-oxidation, anti-aging and other functions, and is used in many fields such as cosmetics, food and biomedical materials. Sericin is rarely used in agriculture. The present invention uses silk protein as a spraying agent for the first time to spray on plant leaves to improve plant drought tolerance, improve plant survival rate under drought stress, plant root biomass and water content, help drought seedling protection, and reduce the risk of drought to crop growth and yield.

[0009] Furthermore, the spraying time is: after the tomato plants grow to five leaves and one heart, spraying is performed at the time when the culture environment changes from darkness to light;

[0010] The culture environment is repeated culture with 16 h of light and 8 h of darkness.

[0011] Furthermore, the silk cocoons are crushed and then boiled in distilled water, and the ratio of the silk cocoons to the distilled water is 5 g to 10 g: 200 mL.

[0012] Furthermore, the concentration of the sericin solution is 0.1 g / L.

[0013] The present invention also provides a sericin solution prepared by the above steps.

[0014] The present invention also provides a use of the sericin solution in tomato plant cultivation, wherein the sericin solution is used to improve the tolerance of tomato plants to drought.

[0015] Furthermore, the sericin solution is used to improve the survival rate of tomato plants under drought stress.

[0016] Furthermore, the sericin solution is used to increase the root biomass and water content of tomato plants under drought stress.

[0017] Furthermore, the sericin solution is used to increase the aboveground biomass and water content of tomato plants under drought stress.

[0018] Furthermore, the tomato is a wild-type tomato Ailsa craig.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention uses silk protein as a spraying agent for the first time to spray on the leaves of tomato plants, thereby increasing the survival rate of tomato plants under drought stress, increasing the root biomass and water content of tomato plants under drought stress, and increasing the aboveground biomass and water content of tomato plants under drought stress. The method of the present invention improves the drought tolerance of plants, helps to protect seedlings under drought, reduces the risks of drought to crop growth and yield, and can be used to develop uses such as saving irrigation water by improving plant drought tolerance. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0022] Figure 1 The effects of different treatments on tomato plants;

[0023] In the figure, A shows the effects of different treatments on the appearance of tomato plants. The NS group means no spraying of any substance, the HS group means foliar spraying of distilled water, and the DS group means foliar spraying of sericin solution;

[0024] B is a statistical chart showing the effects of treatments in NS, HS and DS groups on the survival rate of tomato plants.

[0025] Figure 2 The effect of sericin solution on biomass and water content of tomato plants under drought stress;

[0026] In the figure, A shows the effect of sericin solution on the root fresh weight of tomato plants under drought stress;

[0027] B is the effect of sericin solution on root dry weight of tomato plants under drought stress;

[0028] C is the effect of sericin solution on the root water content of tomato plants under drought stress;

[0029] D is the effect of sericin solution on the relative reduction rate of root fresh weight biomass of tomato plants under drought stress;

[0030] E is the effect of sericin solution on the relative reduction rate of root dry weight biomass of tomato plants under drought stress;

[0031] F is the effect of sericin solution on the aboveground fresh weight of tomato plants under drought stress;

[0032] G is the effect of sericin solution on the aboveground dry weight of tomato plants under drought stress;

[0033] H is the effect of sericin solution on the aboveground water content of tomato plants under drought stress;

[0034] I is the effect of sericin solution on the reduction rate of fresh weight biomass of the aboveground part of tomato plants under drought stress;

[0035] J is the effect of sericin solution on the reduction rate of aboveground dry weight biomass of tomato plants under drought stress. DETAILED DESCRIPTION

[0036] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0037] Embodiment 1:

[0038] 1. Experimental Preparation

[0039] 1. Tomato preparation

[0040] In this example, tomatoes were used as plant test materials. The variety was wild-type tomato Ailsa craig, purchased from Baige Biotechnology. Tomato seeds were disinfected with 70% alcohol by volume for 30 s, 10% sodium hypochlorite by volume for 10 minutes, rinsed with sterile water 5 times, dried with sterile filter paper, and placed in a dark culture at 28°C until germination. The germinated tomato seeds were transplanted into pots and placed in a light incubator for culture. The culture was divided into two stages: stage one was 22000 xl of light, 25°C, 16 h, and stage two was dark without light, 18°C, 8 h. Subsequent experiments were carried out when the tomatoes grew to five leaves and one heart. The culture conditions in the subsequent experiments were repeated in stages one and two.

[0041] 2. Preparation of sericin solution

[0042] Take an appropriate amount of silk cocoons purchased from a local silk company, remove the outermost and innermost layers of silk fibers, and take the clean middle part of the cocoons for the preparation of sericin solution. Cut 5g of clean cocoons into pieces and boil them in 200mL of distilled water for 10 minutes; remove the remaining undissolved silk fibroin, and the resulting solution is the sericin solution. The concentration of the sericin solution was detected using the BCA method, and diluted with distilled water to a concentration of 0.1g / L to obtain the sericin solution for subsequent experiments.

[0043] 2. Experimental Design

[0044] Five-leaf, one-heart tomato plants with the same growth and size were selected for experimental treatment. Three groups of experimental plants were set up, namely NS group, HS group and DS group, with 15 plants in each group. The NS group was a non-spraying group, that is, no substance was sprayed on the leaves; the HS group was a distilled water group, that is, distilled water was sprayed on the front of the leaves; the DS group was a sericin group, that is, sericin liquid was sprayed on the front of the leaves.

[0045] The spraying treatment was carried out according to the above grouping, and the spraying steps were: spraying the front side of the leaves evenly at the time when darkness turns to light in the light incubator, the spraying amount was 10 mL / plant, and spraying was carried out once every 2 days.

[0046] Drought simulation treatment: Drought stress was simulated by applying 20% ​​PEG6000 by mass fraction. 20% PEG6000 was irrigated every 2 days for 12 consecutive days. Spraying and drought treatment were carried out simultaneously.

[0047] In addition, a group of experimental plants with the same growth potential and size were set up as a control under normal growth conditions without drought simulation, serving as a blank control.

[0048] After the above treatments were completed, the biomass and water content of each group of tomatoes were calculated. The specific method is as follows:

[0049] Analysis of biomass and moisture content: After the treatment, the wilting degree of the plants was observed and the survival rate was calculated; the fresh weight of the roots and aboveground parts was weighed respectively, and the dry weight was weighed respectively after drying at 85°C for 5 days.

[0050] The experimental data obtained are calculated according to the following formula:

[0051] Survival rate = (N t -N d ) / N t ;

[0052] Where N d N is the plant that did not survive. t is the total number of tested plants.

[0053] N d The judgment criteria are: if the whole plant withers and wilts, the top of the aboveground part droops, and the plant falls over, it is recorded as a plant that failed to survive the drought treatment. d If the whole plant remains upright, only some leaves on the aboveground part wilt, and there is no lodging, and the whole plant remains green, it is recorded as a plant that survived the drought treatment.

[0054] Moisture content = (Y fw -Y dw ) / Y fw ;

[0055] Where Y fw Y represents fresh weight,dw Indicates dry weight.

[0056] Relative reduction rate of biomass = (Y1-Y2) / Y1

[0057] Wherein, Y1 represents the fresh weight of blank control plants without drought treatment, and Y2 represents the fresh weight of plants treated with drought simulation.

[0058] 3. Experimental Results Analysis

[0059] 1. Effect of sericin solution on the survival rate of tomato plants under drought stress

[0060] Figure 1 A is the growth of plants in the NS, HS and DS groups after 12 days of drought simulation. Figure 1 As shown in A, the majority of the plants in the NS group without any spraying material became yellow and wilted and died, with only a few plants surviving. Figure 1 B, the survival rate is less than 20%.

[0061] Compared with the NS group without any spraying, the HS group sprayed with distilled water on the leaves had a certain effect on alleviating drought damage, and the number of plants withered, wilted and died was reduced. The statistical number is shown in Figure 1 The survival rate of B was 40%. The tolerance of plants in the DS group sprayed with sericin solution on the leaves was significantly enhanced. Although some plants still withered and died, the group grew well and could still grow upright and remain green. The statistical survival rate was Figure 2 The survival rate of B can reach over 80%.

[0062] It can be seen that the survival rate of tomato plants sprayed with sericin solution under drought conditions is as high as over 80%, which is 40% higher than the survival rate of tomato plants sprayed with distilled water and 60% higher than the survival rate of tomato plants not sprayed with any substances. This shows that spraying sericin solution can significantly improve the tolerance of tomatoes to drought.

[0063] 2. Effects of sericin solution on biomass and water content of tomato plants under drought stress

[0064] (1) Effects of sericin solution on root biomass and water content of drought-stressed tomato plants

[0065] according to Figure 2 As can be seen from A and B, the root fresh weight and dry weight of the HS group sprayed with distilled water were slightly higher than those of the NS group without any spraying, but the difference in group performance was not significant. In contrast, the root fresh weight and dry weight of the DS group sprayed with sericin solution were significantly higher than those of the NS group without any spraying, and the fresh weight was significantly higher than that of the HS group sprayed with distilled water.

[0066] like Figure 2 As shown in Figure C, there was no significant difference in the root water content between the HS group sprayed with distilled water and the NS group not sprayed with any substance; however, the root water content of the DS group sprayed with sericin solution was significantly higher than that of the HS group sprayed with distilled water and the NS group not sprayed with any substance, indicating that spraying distilled water cannot increase the root water content of plants under drought conditions, while spraying sericin solution can significantly increase the root water content of tomato plants under drought stress.

[0067] like Figure 2 As shown in D, the relative reduction rate of root fresh weight biomass of the HS group sprayed with distilled water was slightly lower than that of the NS group not sprayed with any substance, but the difference was not significant; while the relative reduction rate of root fresh weight biomass of the DS group sprayed with sericin solution was significantly lower than that of the HS group and the NS group. Therefore, the sericin solution prepared by the present invention significantly improved the drought resistance of tomato plants and alleviated the impact of drought environment on root biomass.

[0068] like Figure 2 As shown in Figure E, the relative reduction rate of root dry weight biomass of the HS group sprayed with distilled water was significantly lower than that of the NS group without any spraying. The relative reduction rate of root dry weight biomass of the DS group sprayed with sericin solution was significantly lower than that of the NS group without any spraying and the HS group sprayed with distilled water. This shows that compared with the test group without any spraying and the distilled water spraying group, spraying sericin solution significantly alleviated the effects of drought stress on the root fresh weight and root dry weight biomass of plants, indicating that spraying sericin solution can significantly reduce the effects of drought on plant root growth.

[0069] (2) Effects of sericin solution on aboveground biomass and water content of tomato plants under drought stress

[0070] The results are as follows Figure 2 As shown in F, the aboveground fresh weight of tomato plants in the DS group sprayed with sericin solution was significantly higher than that in the NS group without any spraying and the HS group sprayed with distilled water; Figure 2 As shown in G, the aboveground dry weight of tomato plants in the DS group sprayed with sericin solution was significantly higher than that in the NS group not sprayed with any substance and the HS group sprayed with distilled water.

[0071] like Figure 2 As shown in Figure 3, the aboveground moisture content of tomato plants in the DS group sprayed with sericin solution was significantly higher than that in the NS group not sprayed with any substance and the HS group sprayed with distilled water.

[0072] like Figure 2As shown in Figure 1, the relative reduction rate of the aboveground fresh weight biomass of tomato plants in the DS group sprayed with sericin solution was significantly lower than the relative reduction rate of the aboveground fresh weight biomass of tomato plants in the NS group not sprayed with any substance and the HS group sprayed with distilled water.

[0073] like Figure 2 As shown in Figure 5J, the relative reduction rate of aboveground dry weight biomass of tomato plants in the DS group sprayed with sericin solution was slightly lower than that in the NS group not sprayed with any substance and the HS group sprayed with distilled water.

[0074] The above results indicate that spraying sericin solution may alleviate the effect of drought stress on the aboveground biomass of plants by increasing the aboveground water content of plants.

[0075] In summary, foliar spraying of sericin solution can significantly improve the tolerance of tomato plants to drought, alleviate the impact of drought on plants, and increase the survival rate of plants under drought stress and the biomass of plants under drought stress. This shows that sericin solution can be used as a spray to improve plant drought resistance, laying the foundation for growing crops in arid environments.

[0076] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0077] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention belong to the scope of equivalent technologies of the present invention, the present invention is also intended to include these modifications and variations.

Claims

1. An application of sericin liquid in tomato plant planting, characterized in that: The sericin solution is used to improve the tolerance of tomato plants to drought; The sericin solution preparation process is as follows: crushing silkworm cocoons and boiling them in distilled water for 10 to 15 minutes to obtain a sericin solution, diluting the solution with distilled water to a concentration of 0.1 g / L to 0.5 g / L to obtain a sericin solution; the ratio of silkworm cocoons to distilled water is 5 g to 10 g: 200 mL; When the tomato plants grow to five leaves and one heart, spray the sericin solution on the front of the leaves at a rate of 10 mL / plant to 15 mL / plant. The spraying time is: after the tomato plants grow to five leaves and one heart, spray at the time when the culture environment changes from darkness to light; The culture environment is repeated culture with 16 hours of light and 8 hours of darkness.

2. The use of the sericin solution in tomato planting according to claim 1, characterized in that: The concentration of the sericin solution is 0.1 g / L.

3. The use of the sericin solution in tomato planting according to claim 1, characterized in that: The sericin solution is used for improving the survival rate of tomato plants under drought stress.

4. The use of the sericin solution in tomato planting according to claim 1, characterized in that: The sericin solution is used to increase the biomass and water content of the root system of tomato plants under drought stress.

5. The use of the sericin solution in tomato planting according to claim 1, characterized in that: The sericin solution is used for increasing the aboveground biomass and water content of tomato plants under drought stress.

6. The use of the sericin solution in tomato planting according to claim 1, characterized in that: The tomato is a wild type tomato Ailsa craig.

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