Composition and application thereof in preparation of product with vagina shrinking effect

Abstract

The invention provides a composition, which is prepared from the following components in parts by weight: 3 to 6 parts of fish collagen peptide, 4 to 8 parts of modified component A, 10 to 20 parts of plant extracting solution, 0.5 to 1.5 parts of xanthan gum and 1 to 2 parts of trehalose, the modified component A is sodium hyaluronate, L-lactic acid, scalp absorbing peptide and lactose water modified prasterone sodium sulfate. The plant extracting solution is obtained by extracting kadsura longepedunculata, orchid, garden balsam stem, fructus cnidii, mangacani, hairyvein agrimony, herba epimedii and motherwort herb. When the composition is applied to the preparation process of the vagina shrinking product, the obtained vagina shrinking product is safe and non-irritant, the vagina shrinking effect is good, and the vagina shrinking product is not easy to rebound.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of vaginal tightening products, and specifically relates to a composition and application thereof in the preparation of products with vaginal tightening effect. Background Art

[0002] The vagina is a muscular hollow organ composed of mucosa, muscle layer and outer membrane. It is about 11 cm long and 2.5 cm in diameter. It is highly stretchable and connects the uterus and external genitalia. It is the female sexual organ and also the channel for menstruation and fetal delivery. There are many muscles and ligaments around the vagina of a normal woman to keep it in a certain tension. There are also many folds on the mucosa of the inner wall of the vagina to increase friction during sexual intercourse.

[0003] Vaginal relaxation is a physiological condition that occurs in women as they age, and the main group of people is postpartum women. Severe vaginal relaxation will reduce the quality of life of the couple. Western medicine believes that this is because natural childbirth or even abortion will cause elastic fibers to break and shrink, resulting in muscle relaxation. Multiparous women have repeated injuries to the birth canal, so the vagina is naturally loose. There are also other cases where the wife's vaginal opening is enlarged by the husband's penis during long-term sexual life, causing the vagina to relax. Vaginal tightening has become a way for women to improve the quality of sexual life of couples. Common methods include vaginal tightening exercises, vaginal tightening products and vaginal tightening surgery. However, vaginal tightening through surgery is risky and can easily cause re-expansion, bleeding, and easy injury.

[0004] Existing vaginal tightening products on the market have side effects during use, are irritating to the human body, have unstable vaginal tightening effects, and have a high rebound rate after the drug effect wears off. Therefore, it is of practical significance to develop a composition for use in vaginal tightening products. Summary of the invention

[0005] The purpose of the present invention is to provide a composition and a preparation method thereof, which uses fish collagen peptides, modified components, plant extracts, etc. as main components of the composition, and applies it to the preparation of vaginal tightening products. The obtained vaginal tightening products are safe, non-irritating, have good vaginal tightening effects, and are not prone to rebound.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A composition comprises the following components in parts by weight: 3-6 parts of fish collagen peptide, 4-8 parts of modified component A, 10-20 parts of plant extract, 0.5-1.5 parts of xanthan gum and 1-2 parts of trehalose.

[0007] Furthermore, the modified component A is sodium prasterone sulfate modified with sodium hyaluronate, L-lactic acid, scalp absorption peptide and lactose.

[0008] Furthermore, the plant extract is extracted from Schisandra chinensis, Herba Cynanchum, Herba Polygoni Multiflori, Fructus Cnidii, Herba Mangakani, Herba Agrimoniae, Herba Epimedii and Herba Leonuri.

[0009] Furthermore, in terms of weight, it includes 3-8 parts of Schisandra chinensis, 1-4 parts of orchid grass, 4-8 parts of radix polygoni lobatae, 3-7 parts of cnidium monnieri, 1-3 parts of mangakani, 2-6 parts of agrimony, 1-3 parts of epimedium and 2-5 parts of Leonurus japonicus.

[0010] A method for preparing a composition comprises the following steps: S1: preparing fish collagen peptide, modified component A and plant extract respectively; S2: First, mix 3-6 parts of fish collagen peptide and 4-8 parts of modified component A, and stir at a temperature of 65-75°C for 30-50 minutes; then add 10-20 parts of plant extract, and continue stirring for 20-40 minutes to obtain a mixed solution A; S3: 0.5-1.5 parts of xanthan gum and 1-2 parts of trehalose are stirred and reacted at a temperature of 90-100° C. for 20-40 minutes; then added into the mixed solution A in step S2, and stirred at a temperature of 45-55° C. for 1-3 hours to obtain the composition.

[0011] Furthermore, the preparation process of the fish collagen peptide in step S1 is as follows: taking the defatted fish meat and sending it into an enzymolysis tank, adding 0.5-1% of papain to the total mass, and enzymolysis for 1-2 hours; controlling the temperature of the enzymolysis tank to 45-55°C, and then adding 0.6-1.2% of the total mass of the composite protease, and continuing the enzymolysis for 1.5-2.5 hours to obtain an enzymolysis mixture; removing the waste residue by centrifugation and filtration to obtain a centrifugal clear liquid; microfiltration of the centrifugal clear liquid can obtain fish collagen peptide.

[0012] Furthermore, the preparation process of the modified component A in step S1 is as follows: by weight, 2-4 parts of sodium hyaluronate, 0.5-1.5 parts of L-lactic acid, 0.6-1.2 parts of scalp absorption peptide, 1-3 parts of lactose hydrate and 5-10 parts of sodium prasterone sulfate are taken, and the reaction is stirred at a temperature of 65-75°C for 65-85 minutes.

[0013] Furthermore, the preparation process of the plant extract in step S1 is: by weight, 3-8 parts of Schisandra chinensis, 1-4 parts of orchid grass, 4-8 parts of Radix Polygoni Multiflori, 3-7 parts of Cnidium monnieri, 1-3 parts of Mangakani, 2-6 parts of Agrimoniae, 1-3 parts of Epimedium and 2-5 parts of Leonurus japonicus are mixed, 80-120 parts of ethanol solution are added thereto, and the mixture is extracted at a temperature of 85-95°C for 2-4h, and the mixture is allowed to stand and filtered to obtain the plant extract.

[0014] Furthermore, 3-6 parts of alkyl glucoside are added in step S3.

[0015] The composition prepared by any one of the above methods is used in the preparation of products for vaginal tightening.

[0016] Fish collagen peptides are small molecule peptides obtained by enzymatic hydrolysis or other methods of processing collagen extracted from fish. Compared with traditional collagen, collagen peptides are more easily absorbed and utilized by the human body. Fish collagen peptides have been widely used in health care products and food industries due to their unique nutritional value and multiple health benefits. Fish collagen peptides are rich in amino acids, especially glycine, proline and hydroxyproline, which are essential for the formation of collagen.

[0017] Sodium hyaluronate is a sodium salt derived from hyaluronic acid (HA) with good water solubility. Hyaluronic acid is a polysaccharide naturally present in the human body, mainly in the skin, connective tissue and eyes, and has important biological functions. Sodium hyaluronate is widely used in cosmetics, skin care products, ophthalmology and joint disease treatment due to its excellent moisturizing properties and various biological effects.

[0018] The main mechanism of action of prasterone sodium sulfate is as an anticholinesterase drug, used to antagonize the neurotoxicity caused by organophosphorus compounds. When organophosphorus pesticides are poisoned, organophosphorus compounds inhibit the activity of cholinesterase, causing acetylcholine to accumulate in nerve synapses, thereby triggering poisoning symptoms. Prasterone sodium can bind to cholinesterase inhibited by organophosphorus, restore its activity, and thus relieve poisoning symptoms.

[0019] Scalp absorption peptides have multiple effects, including promoting blood circulation, promoting hair growth, anti-inflammatory effects, antioxidant effects, repairing damaged tissues, etc. Compared with the prior art, the advantages and beneficial effects of the present invention are: The invention uses sodium hyaluronate, L-lactic acid, scalp absorption peptide and lactose to modify sodium prasterone sulfate, so that the vaginal tightening components can have a sustained release effect, increase the residence time and continuous release, and avoid peak and valley phenomena and side effects; the extract is prepared by using Schisandra chinensis, Herba Cynanchi, Herba Polygoni Multiflori, Fructus Cnidii, Herba Mangakani, Herba Agrimoniae, Herba Epimedii and Herba Leonuri as raw materials, which is harmless to the human body, hygienic in use, and has good vaginal tightening effect and no rebound; the composition has synergistic effects of fish collagen peptide, modified component A, plant extract and other components, is applied to the preparation of vaginal tightening products, and the products have good vaginal tightening effect. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The sources of raw materials used in the present invention are as follows: Papain was from Shanghai Yuanye Biotechnology Co., Ltd., item number S10011; compound protease was from Shanghai Hushi Pharmaceutical Technology Co., Ltd., item number 5311; sodium hyaluronate was from Shanghai Yuanye Biotechnology Co., Ltd. S12034; L-lactic acid was from Shanghai Yuanye Biotechnology Co., Ltd. S11075; lactose was from Zhengzhou Givaudan Chemical Products Co., Ltd., item number 1497; sodium prasterone sulfate was from Wuhan Xinxin Jiali Biotechnology Co., Ltd., item number 5125; xanthan gum was from Jiangsu Weizhirun Biotechnology Co., Ltd., item number 3326; trehalose was from Wuhan Dingcheng Biochemical Reagent Co., Ltd. 1328.

[0022] Example 1 This embodiment provides a method for preparing a composition, comprising the following steps: S1: Put the defatted fish meat into an enzymolysis tank, add 0.5% of papain to the fish meat, and perform enzymolysis at room temperature for 1 hour; control the temperature of the enzymolysis tank to 45°C, add 0.6% of composite protease to the fish meat, and continue enzymolysis for 1.5 hours to obtain an enzymolysis mixture; remove the waste residue by centrifugation and filtration to obtain a centrifugal clear liquid; treat the centrifugal clear liquid with 50um microfiltration to obtain fish collagen peptide; Take 2 parts of sodium hyaluronate, 0.5 parts of L-lactic acid, 0.6 parts of scalp absorption peptide, 1 part of lactose hydrate and 5 parts of sodium prasterone sulfate, stir and react at a temperature of 65°C and a speed of 500r / min for 65 minutes to obtain modified component A; Mix 3 parts of Schisandra chinensis, 1 part of Orchidaceae, 4 parts of Radix Polygoni Multiflori, 3 parts of Fructus Cnidii, 1 part of Mangakani, 2 parts of Agrimoniae Herba, 1 part of Epimedium and 2 parts of Leonurus japonicus, add 80 parts of ethanol solution thereto, extract at 85°C for 2h, let stand, filter to obtain plant extract; S2: First, 3 parts of fish collagen peptide and 4 parts of modified component A were mixed, and stirred at a temperature of 65°C and a speed of 500r / min for 30min; then 10 parts of plant extract were added, and stirring was continued for 20min to obtain mixed solution A; S3: 0.5 parts of xanthan gum and 1 part of trehalose are stirred at 90°C and 600 r / min for 20 minutes; then added into the mixed solution A in step S2, and stirred at 45°C for 1 hour to obtain the composition.

[0023] Example 2 This embodiment provides a method for preparing a composition, comprising the following steps: S1: Take the defatted fish meat and put it into an enzymolysis tank, add papain accounting for 1% of the total mass, and enzymolyze for 2 hours; control the temperature of the enzymolysis tank to 55°C, add a composite protease accounting for 1.2% of the total mass, and continue enzymolysis for 2.5 hours to obtain an enzymolysis mixture; remove the waste residue by centrifugation and filtration to obtain a centrifugal clear liquid; filter the centrifugal clear liquid with 80um microfiltration to obtain fish collagen peptide; Take 4 parts of sodium hyaluronate, 1.5 parts of L-lactic acid, 1.2 parts of scalp absorption peptide, 3 parts of lactose hydrate and 10 parts of sodium prasterone sulfate, stir and react at a temperature of 75°C and a speed of 500r / min for 85min to obtain modified component A; Take 8 parts of Schisandrae Chinensis, 4 parts of Orchidaceae, 8 parts of Radix Polygoni Multiflori, 7 parts of Fructus Cnidii, 3 parts of Mangakani, 6 parts of Herba Agrimoniae, 3 parts of Herba Epimedii and 5 parts of Herba Leonuri, add 120 parts of ethanol solution thereto, extract at 95°C for 4h, let stand, filter to obtain plant extract; S2: First, 6 parts of fish collagen peptide and 8 parts of modified component A were mixed, and stirred at a temperature of 75°C and a speed of 500r / min for 50min; then 20 parts of plant extract were added, and stirring was continued for 40min to obtain mixed solution A; S3: 1.5 parts of xanthan gum and 2 parts of trehalose were stirred at 100° C. and 600 r / min for 40 min; then added into the mixed solution A in step S2, and stirred at 55° C. for 3 h to obtain the composition.

[0024] Example 3 This embodiment provides a method for preparing a composition, comprising the following steps: S1: Put the defatted fish meat into an enzymolysis tank, add 0.8% of papain to the fish meat, and perform enzymolysis for 1.5 hours; control the temperature of the enzymolysis tank to 50°C, add 0.8% of composite protease to the fish meat, and continue enzymolysis for 2 hours to obtain an enzymolysis mixture; remove waste residues by centrifugation and filtration to obtain a centrifugal clear liquid; and perform microfiltration on the centrifugal clear liquid to obtain fish collagen peptide; Take 3 parts of sodium hyaluronate, 1.2 parts of L-lactic acid, 1 part of scalp absorption peptide, 2 parts of lactose hydrate and 8 parts of sodium prasterone sulfate, stir and react at a temperature of 72°C and a speed of 500r / min for 75 minutes to obtain modified component A; Take 6 parts of Schisandra chinensis, 2 parts of orchid grass, 5 parts of Radix Polygoni Multiflori, 5 parts of Fructus Cnidii, 2 parts of Mangakani, 4 parts of Agrimoniae Herba, 2 parts of Epimedium Herba and 4 parts of Leonurus japonicus, mix them, add 100 parts of ethanol solution thereto, extract at a temperature of 90°C for 3.5h, let stand, filter to obtain plant extract; S2: First, 4 parts of fish collagen peptide and 6 parts of modified component A were mixed, and stirred at a temperature of 70°C and a speed of 600r / min for 45min; then 15 parts of plant extract were added, and stirring was continued for 25min to obtain mixed solution A; S3: 1.2 parts of xanthan gum and 1.5 parts of trehalose were stirred at 95°C and 600 r / min for 30 min; then added to the mixed solution A in step S2, and stirred at 50°C for 2.5 h to obtain the composition.

[0025] Comparative Example 1 The difference between this comparative example and Example 1 is that fish collagen peptide is not added in step S1, and the other steps are the same.

[0026] Comparative Example 2 The difference between this comparative example and Example 1 is that the modified component A is not added in step S1, and the other steps are the same.

[0027] Comparative Example 3 The difference between this comparative example and Example 1 is that no plant extract is added in step S1, and the other steps are Comparative Example 4 The difference between this comparative example and Example 1 is that xanthan gum is not added in step S3, and the other steps are the same.

[0028] Comparative Example 5 The difference between this comparative example and Example 1 is that trehalose is not added in step S3, and the other steps are the same.

[0029] Comparative Example 6 Commercially available vaginal tightening products were used for comparison.

[0030] The compositions obtained in various embodiments and comparative examples are applied to the preparation of vaginal tightening products, specifically as follows: 30 g of the composition is dissolved in 10 g of glycerol, and then 0.5 g of 1,2-hexanediol, 0.3 g of caprylhydroxamic acid, 0.6 g of disodium edetate, and 1 g of polyethylene glycol are added, and the mixture is stirred at 45°C, and then 0.2 g of pH regulator triethanolamine is added, and the stirring is continued to obtain a vaginal tightening gel product.

[0031] Performance Test: Mucosal irritation test: According to the WHO recognized vaginal mucosal irritation test method and evaluation criteria, healthy, newly-adult female rabbits of the same species were selected and randomly divided into 3 groups with the same number of people in each group. 2.5 ml of the drug was administered vaginally once a day, and a normal saline blank control was used for continuous medication for 10 days. On the 11th day, the vaginal tissue of the rabbit was taken out, fixed and embedded with 10% formalin, and tissue sections were made for pathological histological examination. According to the different degrees of edema, congestion, leukocyte infiltration, and epithelial ulceration in the vaginal mucosal tissue, each item was recorded with 1-4 points, for a total of 16 points. If the total score is <1, it means that the drug has no vaginal mucosal irritation reaction intensity; 1-<5 means that the drug has very mild vaginal mucosal irritation reaction intensity; 5-<9 means that the drug has mild vaginal mucosal irritation reaction intensity; 9-<12 means that the drug has moderate vaginal mucosal irritation reaction intensity; ≥12 means that the drug has severe vaginal mucosal irritation reaction intensity.

[0032] Vaginal shrinkage test: 30 volunteers are organized to try the product once a day, with 1 ml used each time. The trial period is three months. After the trial, volunteers fill out a questionnaire on the vaginal shrinkage and cleaning effects of the product. The results are rated as good, average, or no effect. Average or above is considered a pass, and a statistically effective rate of more than 75% is considered qualified.

[0033] Microbial antibacterial test: The antibacterial performance was tested according to the "GB15979-2002 Hygiene Standard for Disposable Sanitary Products". The results are shown in Table 1.

[0034] Table 1 Performance test results

[0035] From the above performance test results, it can be seen that Examples 1-3 have good vaginal tightening effect and antibacterial effect without irritation; in particular, the comprehensive performance of Example 3 is the most outstanding. However, because Comparative Examples 1-6 do not adopt the necessary technical solutions, their corresponding performance tests are significantly worse than those of the Examples. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0036] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition, characterized in that The invention comprises the following components in parts by weight: 3-6 parts of fish collagen peptide, 4-8 parts of modified component A, 10-20 parts of plant extract, 0.5-1.5 parts of xanthan gum and 1-2 parts of trehalose.

2. A composition according to claim 1, characterized in that The modified component A is sodium prasterone sulfate modified with sodium hyaluronate, L-lactic acid, scalp absorption peptide and lactose.

3. A composition according to claim 1, characterized in that The plant extract is obtained by extracting Schisandra chinensis, orchid grass, radix polygoni lobatae, fructus cnidii, mangakani, herba agrimonii, epimedium and herba leonuri.

4. A composition according to claim 3, characterized in that In terms of weight, the invention comprises 3-8 parts of Schisandra chinensis, 1-4 parts of Orchidaceae, 4-8 parts of Radix Penicillae, 3-7 parts of Fructus Cnidium monnieri, 1-3 parts of Mangakani, 2-6 parts of Herba Agrimoniae, 1-3 parts of Herba Epimedii and 2-5 parts of Leonurus japonicus.

5. A method for preparing a composition, characterized in that: The steps include: S1: preparing fish collagen peptide, modified component A and plant extract respectively; S2: First, mix 3-6 parts of fish collagen peptide and 4-8 parts of modified component A, and stir at a temperature of 65-75°C for 30-50 minutes; then add 10-20 parts of plant extract, and continue stirring for 20-40 minutes to obtain a mixed solution A; S3: 0.5-1.5 parts of xanthan gum and 1-2 parts of trehalose are stirred and reacted at a temperature of 90-100° C. for 20-40 minutes; then added into the mixed solution A in step S2, and stirred at a temperature of 45-55° C. for 1-3 hours to obtain the composition.

6. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the fish collagen peptide in step S1 is as follows: taking the defatted fish meat and sending it into an enzymolysis tank, adding 0.5-1% of papain to the total mass, and enzymolysis for 1-2 hours; controlling the temperature of the enzymolysis tank to 45-55° C., and then adding 0.6-1.2% of the total mass of a composite protease, and continuing the enzymolysis for 1.5-2.5 hours to obtain an enzymolysis mixture; removing waste residues by centrifugation and filtration to obtain a centrifugal clear liquid; and microfiltration of the centrifugal clear liquid to obtain fish collagen peptide.

7. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the modified component A in step S1 is as follows: by weight, 2-4 parts of sodium hyaluronate, 0.5-1.5 parts of L-lactic acid, 0.6-1.2 parts of scalp absorption peptide, 1-3 parts of lactose hydrate and 5-10 parts of sodium prasterone sulfate are taken, and the mixture is stirred and reacted at a temperature of 65-75°C for 65-85 minutes.

8. The method for preparing a composition according to claim 5, characterized in that: The preparation process of the plant extract in step S1 is as follows: by weight, 3-8 parts of Schisandra chinensis, 1-4 parts of orchid grass, 4-8 parts of Radix Polygoni Multiflori, 3-7 parts of Cnidium monnieri, 1-3 parts of Mangakani, 2-6 parts of Agrimoniae, 1-3 parts of Epimedium and 2-5 parts of Leonurus japonicus are mixed, 80-120 parts of ethanol solution are added thereto, the mixture is extracted at a temperature of 85-95°C for 2-4h, and the mixture is allowed to stand and filtered to obtain the plant extract.

9. The method for preparing a composition according to claim 5, characterized in that: In step S3, 3-6 parts of alkyl glucoside are further added.

10. The composition prepared according to any one of claims 1 to 9 is used in the preparation of products for vaginal tightening.

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