Application of OsMORN1 gene in regulation and control of cold tolerance and / or yield of rice

Abstract

The invention discloses application of an OsMORN1 gene in regulation and control of cold tolerance and / or yield of rice. The invention proves that the method for knocking out the OsMORN1 gene enables the low-temperature germination rate of the mutant at 16 DEG C to be obviously lower than that of a wild type, and meanwhile, the cold resistance of the mutant in the seedling stage is also reduced compared with that of the wild type. Therefore, the OsMORN1 has a positive regulation and control effect in the aspects of regulating the low-temperature germination of the rice seeds and the cold resistance in the seedling stage, and has important research significance in researching a rice cold resistance regulation and control network. Meanwhile, the OsMORN1 gene can be over-expressed or up-regulated, so that the yield, including plant height, spike length, grain length, grain width and thousand grain weight, of crops can be remarkably improved. In general, overexpression or up-regulation of the OsMORN1 gene can improve yield and grain size at the same time.

Description

Technical Field

[0001] The present invention relates to the field of plant gene breeding application technology, and in particular to OsMORN1 Application of genes in regulating rice cold tolerance and yield. Background Art

[0002] Rice is one of the most important food crops, and more than half of the world's population relies on rice as their staple food. Since rice originated in tropical and subtropical regions, it is very sensitive to low temperatures and cold damage. Low temperatures limit the expansion of rice to high altitudes and high latitudes, thereby limiting the rice planting area. Frequent low temperatures in rice-growing areas also seriously affect rice yield and quality. Rice may be attacked by low temperatures throughout its life. The direct impact of cold damage is a decrease in rice fruiting rate, resulting in a significant reduction in yield, which threatens regional food security. Therefore, it is of great significance to improve the cold tolerance of rice.

[0003] In this era of climate change, biotic and abiotic environmental stresses pose a serious threat to continued food production. Other worrisome factors are the decreasing availability of arable land, deteriorating soil quality, and unsustainable farming practices. Therefore, the development of high-yielding crop plants with substantial stress tolerance is a top priority. Therefore, there is an urgent need to breed new varieties of rice that are stress-tolerant and high-yielding. Summary of the invention

[0004] The purpose of the present invention is to overcome the current problems related to the cold tolerance of rice and the difficulty in increasing the yield, and to provide OsMORN1 Application of gene-related biomaterials.

[0005] OsMORN1 The gene is located on chromosome 10, Chr10:22316008-22320296, and the gene locus number is LOC_ Os10g41534 (phytozome database), its full-length genomic sequence is 4288 bp, including 3 exons, 2 introns, 5′ untranslated region (5′UTR), and 3′ untranslated region (3′UTR). Its cDNA is 1404 bp long (SEQ ID NO.1) and encodes 468 amino acids (SEQ ID NO.3).

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions: OsMORN1 Applications of genetically related biomaterials, including: The biological material is any one or more of the following substances A, B, C, D, E, and F: A. OsMORN1 Full-length genomic DNA of a gene or its homologous nucleic acid molecule; B. OsMORN1Gene cDNA or its homologous nucleic acid molecule; C. OsMORN1 Genetically encoded OsMORN1 Protein or its homologous amino acid sequence; D. OsMORN1 A promoter of gene expression or a homologous nucleic acid molecule thereof; E. Substances that can increase the expression and / or activity of substance A, B or D; F. A substance that can increase the expression level and / or activity of substance C.

[0007] Further, the nucleotide sequence of the full-length genomic DNA described in A is as shown in the phytozome database gene locus number: LOC_Os10g41534 sequence is shown.

[0008] Furthermore, the homologous nucleic acid molecule described in A refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology with the full-length genomic DNA.

[0009] Furthermore, the nucleotide sequence of the cDNA described in B is shown in SEQ ID NO.1.

[0010] Furthermore, the homologous nucleic acid molecule described in B refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.1.

[0011] Furthermore, the amino acid sequence of the OsMORN1 protein described in C is shown in SEQ ID NO.3.

[0012] Furthermore, the homologous amino acid sequence described in C refers to an amino acid sequence that has at least 60%, at least 65%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.3.

[0013] Furthermore, the nucleotide sequence of the promoter described in D is shown in SEQ ID NO.2.

[0014] Furthermore, the homologous nucleic acid molecule described in D refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.2.

[0015] Furthermore, the application is any one or more of the following applications 1), 2), and 3): 1) Application of positive regulation of rice cold tolerance; 2) Application of positive regulation of rice yield; 3) Application of improved rice grain shape.

[0016] Furthermore, application 1) includes any one or more of: an application of improving the germination rate of rice seeds at low temperatures, and an application of improving the survival rate of rice seedlings at low temperatures.

[0017] Furthermore, application 2) includes any one or more of the following: an application for improving the fertility of rice spikelets, an application for improving the grain yield per rice plant, an application for increasing the number of effective panicles of rice, an application for increasing the number of filled grains per panicle of rice, an application for increasing the fruit setting rate of rice, an application for increasing the plant height of rice, and an application for increasing the thousand-grain weight of rice.

[0018] Furthermore, application 3) includes any one or more of an application of increasing the grain length of rice and an application of increasing the grain width of rice.

[0019] Furthermore, the substance E is selected from: a recombinant vector, an expression cassette, a transgenic cell line, a transgenic plant tissue or a recombinant bacterium containing a corresponding nucleic acid molecule.

[0020] Furthermore, the vector used in the recombinant vector is selected from the pEarleyGate100 plant expression vector containing the CaMV 35S promoter.

[0021] Furthermore, the substance F is selected from: a recombinant vector, an expression cassette, a transgenic cell line, a transgenic plant tissue or a recombinant bacterium containing a gene encoding a corresponding amino acid sequence.

[0022] Furthermore, the rice includes Nipponbare.

[0023] Compared with the prior art, the present invention has the following advantages and effects: Experiments have shown that OsMORN1 The gene was knocked out in the wild-type variety Nipponbare. The seed germination rate and seedling survival rate of rice at 16°C were significantly lower than those of the wild type. At the same time, the yield was significantly lower than that of the wild type. OsMORN1After the gene was overexpressed in the wild-type variety Nipponbare, the yield was significantly increased compared with the wild type. Specifically, compared with the wild type, the plant height, number of primary / secondary branches, grain length, grain width and thousand-grain weight of the overexpressed plants were significantly increased, which proved that OsMORN1 The possible application potential of genes in agricultural production. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Picture 1 for OsMORN1 Relative expression of genes in their knockout mutants and overexpression strains; ACTIN1 As an internal reference, the wild type OsMORN1 The gene expression level was taken as 1 for comparison; t-test was used for significance analysis, * P<0.05, ** P<0.01.

[0025] Picture 2 The results of the study on the germination rate statistics of OsMORN1 knockout mutants under low temperature stress and the regulation of cold tolerance in rice seedlings are shown in Figure 1. (ab). osmorn1 Cumulative germination rate of mutant seeds under normal temperature and low temperature changes over time; (c). WT, osmorn1 Morphological phenotypes of seedlings after cold treatment; seedlings were grown at 30°C for 3 weeks and then treated at 4°C for 48 h, followed by recovery at 30°C for 7 days; plants were photographed after cold treatment and recovery; scale bar = 5 cm; (d). Survival rates were determined for the mutants and WT shown in (a); values ​​are expressed as the mean ± SD of three biological replicates (n = 30 for each replicate); t-test was used for significance analysis, * P < 0.05, ** P < 0.01.

[0026] Picture 3 Figure 2 shows the results of the study on the regulatory effect of OsMORN1 on rice seed yield; (a). Plant morphology of mutants and WT plants; BAR = 20 cm; (bf). Spikelet fertility (b), grain yield per plant (c), number of effective panicles (d), number of grains per panicle (e) and seed setting rate (f); agronomic traits of 30 rice plants of each line were measured; values ​​obtained from 30 biological replicates are expressed as mean ± SD; asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01and ***P<0.001).

[0027] Picture 4Figures 2 show the plant and panicle types of OsMORN1 overexpressing plants; (a) Plant type of WT plants (Nipponbare NIP) and overexpressing plants, BAR=20 cm; (b) Panicle type of WT plants and overexpressing plants, BAR=5 cm; (c) Panicle length of WT plants and overexpressing plants; (d) Plant height of WT plants and overexpressing plants; Agronomic traits of 30 rice plants of each line were measured; Values ​​obtained from 30 biological replicates are expressed as mean ± SD; Asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01 and ***P<0.001).

[0028] Picture 5 Figure 1 shows the regulation of OSMORN1 on rice yield; (a) Schematic diagram of grain length of WT plants, mutants and overexpression plants, BAR=1 cm; (b) Schematic diagram of grain width of WT plants, mutants and overexpression plants, BAR=1 cm; (c) Grain length of WT plants, mutants and overexpression plants; (d) Grain width of WT plants, mutants and overexpression plants; (e) 1000-grain weight of WT plants, mutants and overexpression plants; Values ​​obtained from 30 biological replicates are expressed as mean ± standard deviation; Asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01 and ***P<0.001). DETAILED DESCRIPTION

[0029] The present invention is further described in detail below in conjunction with embodiments and drawings, but the embodiments of the present invention are not limited thereto.

[0030] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0031] The various raw materials and equipment used in the present invention are all conventional commercially available products and can be directly purchased from the market. The primer sequences used are all synthesized by Shanghai Jierui Bioengineering Co., Ltd.

[0032] OsMORN1 The gene is located on chromosome 10, Chr10:22316008-22320296, and the gene locus number is LOC_ Os10g41534 (phytozome database), its full-length genomic sequence is 4288 bp, including 3 exons, 2 introns, 5′ untranslated region (5′UTR), and 3′ untranslated region (3′UTR). Its cDNA is 1404 bp long (SEQ ID NO.1), encoding 468 amino acids (SEQ ID NO.3).

[0033] The following examples used pEarley Gate 100 The plasmid was purchased from Biovector Plasmid Vector Bacteria Cell Protein Antibody Gene Collection Center (NTCC).

[0034] Embodiment 1, OsMORN1 Construction of gene knockout and / or overexpression vectors 1) OsMORN1 CDS amplification of genes Using cultivated rice Nipponbare leaves as materials, total RNA from seedlings was extracted using a plant total RNA extraction kit (TIANGEN, DP432); using total RNA as a template, a cDNA sequence was synthesized using the lnRcute lncRNA cDNA first-strand synthesis kit (TIANGEN, KR202); using cDNA as a template, M1-F and M1-R as primers, PCR amplification was performed to obtain the PCR amplification product OsMORN1. After sequencing, the nucleotide sequence of the PCR product is shown in SEQ ID NO.1.

[0035] The sequences of M1-F and M1-R are as follows: M1-F: 5'-ATGCACCACCACCACCACCTC-3' M1-R: 5'-TCACACTACTGACAACGGTAG-3'.

[0036] 2) OsMORN1 Construction of knockout vectors and / or overexpression vectors according to OsMORN1 Based on the characteristics of gene structure, two specific targets were selected from exon 1 and exon 2, and the knockout vector was constructed using the CRISPR / Cas9 system described in “CRISPR-GE: A convenient software toolkit for CRISPR-based genome editing”. OsMORN1 Knockout vector. The primer sequences used are as follows: gRT1-MORN1: 5'-CCCCGTCATGACCTCCTCCTGTTTTAGAGCTAGAAAT-3' U3T1-MORN1: 5'-AGGAGGAGGTCATGACGGGTGCCACGGATCATCTGC-3' gRT2-MORN1: 5'-TCACAGCTATAGGGGACCCAGTTTTAGAGCTAGAAAT-3'.

[0037] Using the above OsMORN1 The full-length CDS sequence of the gene was constructed using the Gateway vector system. OsMORN1 The full-length CDS of the gene was constructed in the presence of the CaMV 35S promoter. pEarley Gate 100 Plant expression vectors are then used to obtain OsMORN1 Overexpression vector.

[0038] The obtained OsMORN1 The knockout vector and / or overexpression vector are transformed into rice callus by Agrobacterium, and the rice callus containing the knockout vector is selectively cultured on a medium containing Hyg; the rice callus containing the overexpression vector is selectively cultured on a medium containing Bar, and the culture is carried out to obtain OsMORN1 Mutant and / or overexpressed transgenic plants. Replace the selection medium after 2 weeks, and culture for another 2-3 weeks. Select the vigorously growing and dense callus and transfer it to the pre-differentiation medium. Culture it in the dark at 26-28℃ for about 1 week. Then transfer the callus to the differentiation medium and culture it in the light at 26-28℃ until the regenerated plants are differentiated. Extract the total DNA of the transgenic rice, use the DNA as a template, and design specific primers based on the tag gene of the vector and our target gene for PCR amplification. The transgenic positive plants are judged based on the amplified bands and sequencing. The selected transgenic positive plants are OsMORN1 Mutant and / or overexpressing transgenic plants.

[0039] 3) OsMORN1 Expression verification of mutants and / or overexpression plants Based on the above (2) OsMORN1 Total RNA was extracted from seedlings of mutants and / or overexpressed transgenic plants using a plant total RNA extraction kit (TIANGEN, DP432); total RNA was used as a template to synthesize cDNA sequences using the lnRcute lncRNAcDNA first-strand synthesis kit (TIANGEN, KR202). OSMORN1 Real-time quantitative fluorescence PCR was performed to detect the OSMORN1 The expression level of the gene at the transcriptional level. The experiment was repeated 3 times and the results were averaged. The primer sequences used are as follows: OsActin-F: 5'- TGGCATCTCTCAGCACATTCC-3' OsActin-R: 5'- TGCACAATGGATGGGTCAGA-3' OsMORN1-qPCR-F: 5'-TGGCCACTACCATTTCAGG-3' OsMORN1-qPCR-R: 5'-GACCATTGGCAAAACTGTAGACT-3'.

[0040] Example 2: Cold tolerance test of mutants and wild type 1) Evaluation criteria for seed germination The panicles of mature plants in the experimental field were cut off, and the seeds were dried in a glass greenhouse for one week before threshing to obtain seeds. Seed germination was carried out according to the method described in "Influence of isopropylmalate synthase OsIPMS1 on seedvigour associated with amino acidand energy metabolism in rice". When the radicle protruded the seed coat, the seeds were considered to have germinated. When the root length reached the seed length and the shoot length reached half the seed length, the seedlings were considered to have survived. Each experiment had at least three biological replicates.

[0041] 2) Cold resistance test at the budding stage Cold tolerance at the germination stage was evaluated by the germination rate of 30 seeds. Homozygous mutant seeds and wild-type seeds were placed in moist petri dishes and cultured at 16°C for 13 days. The control group was also cultured at 30°C for 10 days. The germination rate of seeds in the treatment and control was recorded every day. Three biological replicates were performed for each sample.

[0042] 3) Cold tolerance test at the seedling stage To evaluate cold tolerance at the seedling stage, 3-week-old mutant and wild-type seedlings were treated at 4 °C for 2 days. After 7 days of recovery at 30 °C, the survival rate was determined as the percentage of surviving seedlings to the total number of seedlings. Sixteen biological replicates were performed for each sample. All experiments were repeated at least three times.

[0043] Example 3: Agronomic trait measurement and statistical analysis All plants were planted in the field with a vertical spacing of 10-15 cm and a horizontal spacing of 13-20 cm. After the seeds matured, phenotypic photos and measurements were taken as follows: Analysis of ears: 30 individual plants were collected for statistics on individual plant yield, number of effective ears, number of grains per ear, ear length, primary stalks, secondary stalks, and fruit setting rate.

[0044] Analysis of plant height: Collect 30 individual plants for plant height measurement, start measuring from the above-ground part of the rice, and straighten the ear close to a ruler until the highest point of the ear is the plant height data of the individual plant.

[0045] Biomass analysis: All above-ground tissues of 30 individual plants were collected and dried at 45-50°C. After drying, the tissues were weighed and their length and / or width were measured.

[0046] All data were analyzed using Graphpad Prism8 software, standard deviation was expressed as SD, and t-test was used for analysis of variance.

[0047] Picture 1 for OsMORN1 Relative expression of genes in their knockout mutants and overexpression strains; ACTIN1 As an internal reference, the wild type OsMORN1 The gene expression level was taken as 1 for comparison; t-test was used for significance analysis, * P<0.05, ** P<0.01.

[0048] Picture 2 The results of the study on the germination rate statistics of OsMORN1 knockout mutants under low temperature stress and the regulation of cold tolerance in rice seedlings are shown in Figure 1. (ab). osmorn1 Cumulative germination rate of mutant seeds under normal temperature and low temperature changes over time; (c). WT, osmorn1 Morphological phenotypes of seedlings after cold treatment; seedlings were grown at 30°C for 3 weeks and then treated at 4°C for 48 h, followed by recovery at 30°C for 7 days; plants were photographed after cold treatment and recovery; scale bar = 5 cm; (d). Survival rates were determined for the mutants and WT shown in (a); values ​​are expressed as the mean ± SD of three biological replicates (n = 30 for each replicate); t-test was used for significance analysis, * P < 0.05, ** P < 0.01.

[0049] Picture 3 Figure 2 shows the results of the study on the regulatory effect of OsMORN1 on rice seed yield; (a). Plant morphology of mutants and WT plants; BAR = 20 cm; (bf). Spikelet fertility (b), grain yield per plant (c), number of effective panicles (d), number of grains per panicle (e) and seed setting rate (f); agronomic traits of 30 rice plants of each line were measured; values ​​obtained from 30 biological replicates are expressed as mean ± SD; asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01and ***P<0.001).

[0050] Picture 4Figures 2 show the plant and panicle types of OsMORN1 overexpressing plants; (a) Plant type of WT plants (Nipponbare NIP) and overexpressing plants, BAR=20 cm; (b) Panicle type of WT plants and overexpressing plants, BAR=5 cm; (c) Panicle length of WT plants and overexpressing plants; (d) Plant height of WT plants and overexpressing plants; Agronomic traits of 30 rice plants of each line were measured; Values ​​obtained from 30 biological replicates are expressed as mean ± SD; Asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01 and ***P<0.001).

[0051] Picture 5 Figure 1 shows the regulation of OSMORN1 on rice yield; (a) Schematic diagram of grain length of WT plants, mutants and overexpression plants, BAR=1 cm; (b) Schematic diagram of grain width of WT plants, mutants and overexpression plants, BAR=1 cm; (c) Grain length of WT plants, mutants and overexpression plants; (d) Grain width of WT plants, mutants and overexpression plants; (e) 1000-grain weight of WT plants, mutants and overexpression plants; Values ​​obtained from 30 biological replicates are expressed as mean ± standard deviation; Asterisks are significantly different from WT values ​​by t-test (*P<0.05, **P<0.01 and ***P<0.001).

[0052] from Picture 2 It can be seen that knocking out OsMORN1 After the gene was added, its low-temperature germination rate at 16°C was significantly lower than that of the wild type, and the cold tolerance of the mutant at the seedling stage also showed a downward trend compared with the wild type. OsMORN1 It has a positive regulatory effect on regulating the low-temperature germination of rice seeds and the cold tolerance of the seedling stage, which is of great significance for studying the cold tolerance regulatory network of rice. Picture 3 It can be seen that the mutant has significantly decreased spikelet fertility, grain yield per plant, number of effective ears, number of grains per ear, and fruit setting rate compared with the wild type. Picture 4 Overexpression or upregulation can be seen OsMORN1 The gene can significantly improve crop yields, including significantly increasing plant height and ear length compared to the wild type. Picture 5 Overexpression or upregulation can be seen OsMORN1 / 2 After the gene was upregulated, the grain length, grain width and 1000-grain weight of the overexpressing plants were significantly increased compared with the wild type, while the grain length, grain width and 1000-grain weight of the mutants were not significantly different from those of the wild type. In general, overexpression or upregulation of OsMORN1 / 2 Genes can improve both yield and kernel size. OsMORN1 / 2 Genes have potential in agricultural production.

[0053] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. OsMORN1 Application of gene-related biomaterials, characterized by: The biological material is any one or more of the following substances A, B, C, D, E, and F: A. OsMORN1 Full-length genomic DNA of a gene or its homologous nucleic acid molecule; B. OsMORN1 Gene cDNA or its homologous nucleic acid molecule; C. OsMORN1 OsMORN1 protein encoded by the gene or its homologous amino acid sequence; D. OsMORN1 A promoter of gene expression or a homologous nucleic acid molecule thereof; E. Substances that can increase the expression and / or activity of substance A, B or D; F. A substance that can increase the expression amount and / or activity of substance C; The application is any one or more of the following applications 1), 2), and 3): 1) Application of positive regulation of rice cold tolerance; 2) Application of positive regulation of rice yield; 3) Application of improved rice grain shape.

2. according to claim 1 OsMORN1 Application of gene-related biomaterials, characterized by: Application 1) includes: any one or more of the application of improving the germination rate of rice seeds at low temperatures and the application of improving the survival rate of rice seedlings at low temperatures; Application 2) includes: any one or more of the following: applications for improving the fertility of rice spikelets, applications for improving the grain yield per rice plant, applications for increasing the number of effective rice panicles, applications for increasing the number of grains per panicle of rice, applications for increasing the fruit setting rate of rice, applications for increasing the plant height of rice, and applications for increasing the thousand-grain weight of rice; Application 3) includes: any one or more of an application for increasing the grain length of rice and an application for increasing the grain width of rice.

3. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The nucleotide sequence of the full-length genomic DNA described in A is as shown in the phytozome database gene locus number LOC_ Os10g41534 The sequence is shown; The homologous nucleic acid molecule described in A refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology with the full-length genomic DNA.

4. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The nucleotide sequence of the cDNA described in B is shown in SEQ ID NO.1; The homologous nucleic acid molecule described in B refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.

1.

5. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: Described in C OsMORN1 The amino acid sequence of the protein is shown in SEQ ID NO.3; The homologous amino acid sequence described in C refers to an amino acid sequence that has at least 60%, at least 65%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.

3.

6. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The nucleotide sequence of the promoter described in D is shown in SEQ ID NO.2; The homologous nucleic acid molecule described in D refers to a nucleic acid molecule that has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology to the sequence shown in SEQ ID NO.

2.

7. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The substance E is selected from: a recombinant vector containing a corresponding nucleic acid molecule, an expression cassette, a transgenic cell line, a transgenic plant tissue or a recombinant bacterium.

8. According to claim 7 OsMORN1 Application of gene-related biomaterials, characterized by: The vector used in the recombinant vector is selected from the pEarley Gate100 plant expression vector containing the CaMV 35S promoter.

9. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The substance F is selected from: a recombinant vector, an expression cassette, a transgenic cell line, a transgenic plant tissue or a recombinant bacterium containing a coding gene of a corresponding amino acid sequence.

10. The method according to claim 1 or 2 OsMORN1 Application of gene-related biomaterials, characterized by: The rice includes Nipponbare.

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