A twin black soil, its preparation method and application

Abstract

This invention provides a twin-species black soil, its preparation method, and its application. It belongs to the field of regenerated black soil technology. This invention utilizes a self-developed multifunctional matrix rod fermentation system, combined with a cross-species dual-biological food chain, to decompose and consume waste plants, transforming them into regenerated black soil in a very short time. It achieves the goal of converting waste plant humus into characteristic black soil within 14-17 days, greatly improving the efficiency and effectiveness of industrialized black soil production. It simplifies the treatment of waste plants and straw, facilitates the rapid restoration of degraded black soil, and increases soil biological activity, fertility, organic matter, and organic carbon content.

Description

Technical Field

[0001] This invention relates to the field of regenerated black soil technology, and more specifically to a twin black soil, its preparation method, and its application. Background Technology

[0002] China has 1.8 billion mu of arable land, of which 520 million mu are black soil, accounting for 28% of the total arable land. Black soil is a unique treasure bestowed upon humankind by nature. It is a clayey soil with strong expansion, contraction, and disturbance characteristics, possessing excellent properties and high fertility, making it ideal for plant growth. The formation of black soil in nature is slow; it takes 300-400 years of ecological cycles and natural accumulation to complete a 1-centimeter layer of black soil. Even ordinary waste plants take at least 1-2 years to naturally transform into humus black soil. With rapid agricultural development and excessive exploitation of black soil, the trend of continuous reduction in high-quality arable land and black soil is intensifying. Over-cultivation, excessive use of chemical fertilizers, and abnormal climate have led to soil erosion, soil degradation, a sharp decline in organic matter, and increasingly serious problems of black soil becoming thinner, less fertile, and harder. Curbing black soil degradation and rapidly restoring and conserving black soil have attracted significant attention from society.

[0003] Since natural black soil is located in cold and temperate zones, it is technically challenging to artificially modify and create black soil using biological methods under low temperatures. Currently, there are no publicly available, mature, industrialized methods for artificially regenerated humus black soil, either domestically or internationally.

[0004] Therefore, how to develop a method for preparing recycled humic black soil with lower raw material costs, greater safety, and faster and more efficient production is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a dual-biological black soil, its preparation method, and its application. With the help of dual biological processes and advanced treatment technology, waste plant materials are transformed into regenerated black soil in a very short time, achieving the goal of converting waste plant humus into characteristic black soil within 14-17 days. This is beneficial for the rapid restoration of degraded black soil.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A method for preparing twin black soil includes the following steps:

[0008] (1) Low-temperature fermentation: Take plant waste, crush it, add it to the multi-functional matrix rod fermentation system, inject black soil inducing bacteria and low-temperature fermentation bacteria, and control the fermentation temperature within the range of 35-40℃ through the heat recovery effect of the matrix rod. Low-temperature fermentation for 72 hours yields low-temperature fermented material.

[0009] (2) High-temperature fermentation: High-temperature fermentation bacteria and air are injected into the low-temperature fermentation material through a multi-functional matrix rod fermentation system, and the temperature is controlled at 70-75℃. High-temperature fermentation is carried out for 24 hours to obtain high-temperature fermented material.

[0010] (3) Low-temperature fermentation again: The temperature is adjusted to 35-40℃ through the heat recovery effect of the matrix rod. Black soil inducing bacteria and low-temperature fermentation bacteria are injected into the obtained high-temperature fermentation material. Low-temperature fermentation is carried out for 72 hours. The material temperature is adjusted to 28℃ again through the heat recovery effect of the matrix rod and then put into use.

[0011] (4) Dual-organism culture: After injecting black soil inducing bacteria, low-temperature fermentation bacteria and air into the material obtained in step (3) through a multi-functional matrix rod fermentation system, the material is transferred to a culture bed and low-temperature resistant saprophytic earthworms are placed in it. Under the action of dual organisms, the material is cultured at 15-28℃ for 7-10 days. The earthworms and soil are separated to obtain regenerated black soil.

[0012] Preferably, the composition of the black soil inducing bacteria in steps (1), (3) and (4) is: 35% Aspergillus niger and 65% Bacillus subtilis; the composition of the low-temperature fermentation bacteria is: 25% Rhizobium, 45% Acetobacter stenosis, 20% Lactobacillus and 10% Methanococcus sanguinalis.

[0013] Preferably, the plant waste in step (1) includes, but is not limited to, plant stalks, straw, discarded tree branches and leaves, wormwood, weeds and sawdust.

[0014] Preferably, the particle size of the plant waste in step (1) is 100-200 mesh; the addition ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria is 20% and 80%, respectively; based on plant waste with 40% humidity, 0.5L of mixed bacterial solution of black soil inducing bacteria and low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8 cfu / ml.

[0015] Preferably, the high-temperature fermentation bacteria in step (2) include thermophilic bacteria and / or thermophilic actinomycetes. The amount of high-temperature fermentation bacteria added is calculated based on the material with 40% humidity, with 0.5L of high-temperature fermentation bacteria liquid added per ton, and the concentration of the high-temperature fermentation bacteria liquid is 10^8 cfu / ml. The amount of air added is calculated based on the material with 40% humidity, with 20 cubic meters of fresh air added per ton.

[0016] Preferably, the addition ratios of black soil-inducing bacteria and low-temperature fermentation bacteria in step (3) are 30% and 70%, respectively; based on a material with 40% humidity, 0.5L of mixed bacterial solution of black soil-inducing bacteria and low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8 cfu / ml.

[0017] Preferably, the addition ratio of black soil inducing bacteria and low-temperature fermentation bacteria in step (4) is 20% and 80%, respectively. Based on the material with 40% humidity, 0.5L of mixed bacterial solution of black soil inducing bacteria and low-temperature fermentation bacteria is added per ton; the concentration of the mixed bacterial solution is 10^8 cfu / ml.

[0018] Preferably, in step (4), the amount of low-temperature resistant saprophytic earthworms added shall not be less than 10% of the total amount of material; the amount of air added shall be calculated based on the material with 40% humidity, with 20 cubic meters of fresh air added per ton.

[0019] Another object of the present invention is to provide the application of the above method in the preparation of twin black soils.

[0020] Another object of the present invention is to provide: twin black soil prepared by the above method.

[0021] Another object of the present invention is to provide the application of the above-mentioned twin black soil in soil improvement and crop cultivation.

[0022] As can be seen from the above technical solution, compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) This invention utilizes a self-developed multifunctional matrix rod fermentation system and combines a cross-species dual-biological food chain to decompose and consume waste plants, transforming waste plants into regenerated black soil in a very short time. This achieves the goal of transforming waste plant humus into characteristic black soil in 15-20 days, greatly improving the efficiency and benefits of industrialized black soil production. It also makes the treatment of waste plants and straw simple and easy, which is conducive to the rapid restoration of degraded black soil, increasing soil biological activity, soil fertility, organic matter, and organic carbon content.

[0024] (2) It avoids a series of environmental problems such as carbon dioxide emissions and air pollution caused by burning waste plants and straw; and fills the gap in the black soil industry supply chain caused by the inability to trade black soil after the implementation of the Black Soil Protection Law.

[0025] (3) In the decomposition and transformation of waste plants in black soil by the microbial community in the dual organisms, the black soil inducing bacteria play a role in establishing the organic carbon source structure of black soil and producing humic acid; the low-temperature fermentation bacteria decompose waste plant materials to provide edible nutrition for earthworms and act as a catalyst for the auxiliary digestion and decomposition of food in the food chain of carrion earthworms. Low-temperature tolerant carrion earthworms are mixed earthworms of two or more species. The mixed treatment of waste plant materials catalyzed by low-temperature bacteria by two earthworm species can stimulate the territorial attributes of earthworms, making them accelerate the competition for food and improve the feeding efficiency of earthworms. The two earthworm species excrete a large amount of substances rich in humic acid, humic acid, organic carbon and other substances after consuming the material catalyzed by low-temperature bacteria. After digestion in the intestinal cavity, the earthworms excrete humus, mucus and mineral colloids. The organic colloids constitute the granular structure of earthworm castings black soil. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0027] Figure 1 For example: Method flowchart;

[0028] Figure 2 Here is a plan view of the insulated fermentation tank.

[0029] Figure 3 Here is a top view of the insulated fermentation tank.

[0030] Figure 4 Here is a three-dimensional cross-sectional view of an insulated fermentation tank.

[0031] Figure 5 For example: Matrix bar disassembly and assembly diagram;

[0032] Figure 6 Diagram of an integrated cultivation and harvesting device;

[0033] Figure 7 Diagram of a photosensitive earthworm repellent device;

[0034] Figures 2-5 In the middle, 1-Insulated fermentation tank, 2-Material inlet and outlet opening, 3-Pit base, 4-Matrix rod hoisting and landing frame, 5-Hoisting cable, 6-Matrix rod, 7-Water inlet pipe, 8-Water outlet pipe, 9-Liquid supply and gas supply pipe, 61-Shell, 62-Inner upper end cover, 63-Bottom cover, 64-Heat absorption and heat transfer copper pipe, 65-Heat absorption and heat transfer copper pipe inlet, 66-Heat absorption and heat transfer copper pipe outlet, 67-Interface for heat absorption and heat transfer copper pipe connection installation, 68-Heat absorption and heat transfer copper pipe connection, 69-Liquid supply and gas supply pipe connection hole, 70-Gas outlet and liquid outlet hole, 71-Hanging ring, 72-Heat absorption and heat transfer copper pipe fixing clamp, and 73-Sensing and control system;

[0035] Figures 6-7 In the middle, 1-harvesting equipment, 2-photosensitive earthworm repellent equipment, 3-connecting rod, 4-culture bed, 11-harvesting box, 12-harvesting impeller, 13-roller conveyor belt, 14-conveyor auger, 15-lifting device, 21-light source, 22-roller cage, 23-shaft head seat, 24-bearing, 25-fiber optic column, 41-bed frame, 42-trough, 43-track, 44-track wheel. Detailed Implementation

[0036] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0037] The multifunctional matrix rod fermentation system and the integrated cultivation and harvesting device used in the embodiments of this invention are two devices independently developed by the inventor. They were applied for invention patents on the same day as this application, and the corresponding patent names are: a multifunctional matrix rod fermentation system and its application; and a photosensitive earthworm interactive separation and harvesting device, system and its application.

[0038] The composition of the black soil inducing bacteria used in Examples 1-3 is: 35% Aspergillus niger and 65% Bacillus subtilis; the composition of the low-temperature fermentation bacteria is: 25% Rhizobium, 45% Acetobacter stenosis, 20% Lactobacillus and 10% Methanococcus pyogenes; the high-temperature fermentation bacteria include thermophilic bacteria and / or thermophilic actinomycetes.

[0039] Example 1

[0040] A method for preparing twin black soil includes the following steps:

[0041] (1) Low-temperature fermentation: Take 1 ton of plant waste and crush it to 200 mesh, adjust the material moisture to 40%, add it to the multi-functional matrix bar fermentation system, inject 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80%, the bacterial solution concentration is 10^8 cfu / ml, control the temperature at 40℃ through the heat recovery effect of the matrix bar, and ferment at low temperature for 72 hours to obtain low-temperature fermented material;

[0042] (2) High-temperature fermentation: 0.5L of high-temperature actinomycetes are injected into the low-temperature fermentation material through a multi-functional matrix rod fermentation system. The bacterial concentration is 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air are injected and the temperature is controlled at 75℃. High-temperature fermentation is carried out for 24 hours to obtain high-temperature fermented material.

[0043] (3) Low-temperature fermentation again: The temperature is controlled at 40°C by the heat recovery effect of the matrix rod. 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% is injected into the high-temperature fermentation material. The bacterial solution concentration is 10^8 cfu / ml. Low-temperature fermentation is carried out for 72 hours. The material temperature is adjusted to 28°C by the heat recovery effect of the matrix rod again and then put into use.

[0044] (4) Dual-biological culture: 0.5L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria at a ratio of 20% and 80% was injected into the material obtained in step (3) through a multifunctional matrix rod fermentation system. The bacterial solution concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air were injected. The material was then transferred to a culture bed, and 10% of the total weight of Eisenia fetida and Heterolipa worms were added. Under the action of the dual-biological system, the material was cultured at 15℃ for 10 days. The earthworms were then separated using a photosensitive earthworm interactive separation and harvesting device to obtain regenerated black soil. Figure 1 ).

[0045] In this embodiment, steps (1)-(3) use a multifunctional matrix bar fermentation system. Figures 2-5 The system is designed to perform low-temperature-high-low-temperature fermentation on plant waste materials. It includes an insulated fermentation tank and a matrix rod. The insulated fermentation tank has several pits that are flush with the ground, which can be used to vertically install the matrix rods.

[0046] The multi-functional matrix bar fermentation system is equipped with a sensing and control system. During the fermentation process, it controls the flow of water medium in the heat absorption and heat transfer copper tubes of the matrix bars, and outputs or pours in biological heat energy to realize the recovery of biological heat energy and temperature control of fermentation materials. This can reduce the fermentation time by 24 hours, which is both energy-saving and improves work efficiency.

[0047] Meanwhile, the matrix rod is also equipped with connection holes for liquid supply and gas filling pipes, as well as multiple liquid supply and gas filling outlets located at different heights on the surface of the matrix rod.

[0048] When the multi-functional matrix bar fermentation system is running, the plant waste to be fermented is placed in the insulated fermentation tank. Under the command of the temperature sensor, the heat-absorbing and heat-transferring copper pipes transfer the heat energy of the microorganisms through the water medium, or introduce the stored heat energy, so that the plant waste can quickly ferment at the optimal temperature. At this time, the matrix bars play a role in heat recovery of microbial energy and temperature regulation and maintenance of fermentation materials. At the same time, the matrix bars supply liquid and aeration channels provide real-time aeration, water injection for humidity adjustment, bacterial solution replacement, and temperature control for the waste plant materials in fermentation tanks at different depths. All fermentation processes are completed in one stop, eliminating the need for complex steps such as turning, turning, repeated splashing of bacterial solution, and multiple stirring in traditional fermentation processes. This reduces the investment in production equipment such as turning machines, mixers, and ventilation and cooling systems, saving equipment investment funds, greatly shortening fermentation time, and improving work efficiency.

[0049] In this embodiment, step (4) involves dual biological culture and separation harvesting, and the equipment used is an integrated culture and harvesting device. Figures 6-7 ):

[0050] The cultivation bed used includes: a bed frame, a trough, and a track. The trough is fixed on the bed frame. The track consists of two tubular tracks, which are set above the bed frame and the trough and are integrally formed with the bed frame. The photosensitive vermicomposting interactive separation and harvesting equipment moves along the track above the trough to separate and harvest the twin black soil.

[0051] The photosensitive earthworm interactive separation and harvesting equipment used includes a harvesting device and a photosensitive earthworm repelling device. The harvesting device is located behind the photosensitive earthworm repelling device and is connected to the photosensitive earthworm repelling device via a connecting rod.

[0052] The photosensitive earthworm repellent device is equipped with a roller cage, a shaft head seat, a bearing, and a light source; the shaft head seat and bearing are located at the center of the circular cross-section of the roller cage, so that the roller cage rolls around the shaft head seat as the center by passing through the bearing.

[0053] The light source is located inside the drum; a beam light source is installed on the outer wall of the drum.

[0054] The light source is a 100-200W 760nm lamp;

[0055] The harvesting equipment includes a harvesting box, a harvesting impeller, a roller conveyor belt, and a conveying auger. The harvesting impeller, roller conveyor belt, and conveying auger are connected together. The harvesting impeller transfers the harvested material to the roller conveyor belt, and the conveying auger transfers the harvested material to the harvesting box.

[0056] When the photosensitive earthworm interactive separation and harvesting equipment is running, the harvesting equipment moves at a constant speed on the track. Simultaneously, a photosensitive earthworm-repelling device, located in front of the machine and placed on the black soil in the trough, rolls forward. During its movement, the nano-lights inside the rolling cage are illuminated. The light emitted from the 760-nanometer spectrum has extremely strong penetrating power and is also the spectrum range most sensitive to earthworms. The light is introduced into the soil layer through fiber optic columns. The soil layer is light-sensitive at a depth of 3-5 cm. Upon sensing the light, earthworms will actively burrow downwards to avoid it. By the time the soil-removing impeller reaches the area passed by the photosensitive earthworm-repelling rolling cage, the earthworms have already burrowed to a depth of 3 mm. The harvesting impeller removes this 3 cm of soil and transfers it to the synchronously running harvesting box. With each round trip, the photosensitive earthworm interactive separation and harvesting equipment moves 3 cm deeper into the soil layer. Once the earthworms' avoidance limit is reached, the equipment stops working, and new material is added to the culture bed to begin the next cycle of dual-biological culture. This allows for the natural separation of earthworms and soil without harming the earthworms, and the earthworms can be recycled multiple times.

[0057] Example 2

[0058] A method for preparing twin black soil includes the following steps:

[0059] (1) Low-temperature fermentation: Take 1 ton of corn stalks, crush them to 100 mesh, adjust the material moisture to 40%, add them to the multi-functional matrix bar fermentation system, inject 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80%, the bacterial solution concentration is 10^8 cfu / ml, control the temperature at 35℃ through the heat recovery effect of the matrix bar, and ferment at low temperature for 72h to obtain low-temperature fermented material;

[0060] (2) High-temperature fermentation: 0.5L of thermophilic bacteria are injected into the low-temperature fermentation material through a multi-functional matrix rod fermentation system. The bacterial concentration is 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air are injected and the temperature is controlled at 70℃. High-temperature fermentation is carried out for 24 hours to obtain high-temperature fermented material.

[0061] (3) Low-temperature fermentation again: The temperature is controlled at 35°C by the heat recovery effect of the matrix rod. 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% is injected into the high-temperature fermentation material. The bacterial solution concentration is 10^8 cfu / ml. Low-temperature fermentation is carried out for 72 hours. The material temperature is adjusted to 28°C by the heat recovery effect of the matrix rod again and then put into use.

[0062] (4) Dual-biological culture: 0.5L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria at a ratio of 20% and 80% was injected into the material obtained in step (3) using a multifunctional matrix rod fermentation system. The bacterial solution concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air were injected. The material was then transferred to a culture bed, and 10% of the total weight of Eisenia fetida and Heterolipa worms were added. Under the action of the dual-biological system, the material was cultured at 15-28℃ for 7-10 days. The earthworms were then separated using a photosensitive earthworm-interactive separation and harvesting device to obtain regenerated black soil. The equipment used in this embodiment is the same as in embodiment 1.

[0063] The effective components of the twin black soil prepared in Example 2 were determined, and the experimental results are as follows: the organic matter content was 119 g / kg, the total nitrogen was 0.513%, the total phosphorus was 1.92 g / kg, and the total potassium was 2.13%.

[0064] Example 3

[0065] A method for preparing twin black soil includes the following steps:

[0066] (1) Low-temperature fermentation: Take 1 ton of soybean straw, crush it to 150 mesh, adjust the material moisture to 40%, add it to the multi-functional matrix bar fermentation system, inject 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 20% and 80%, the bacterial solution concentration is 10^8 cfu / ml, control the temperature at 37℃ through the heat recovery effect of the matrix bar, and ferment at low temperature for 72 hours to obtain low-temperature fermented material;

[0067] (2) High-temperature fermentation: 0.5L of thermophilic bacteria are injected into the low-temperature fermentation material through a multi-functional matrix rod fermentation system. The bacterial concentration is 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air are injected and the temperature is controlled at 73℃. High-temperature fermentation is carried out for 24 hours to obtain high-temperature fermented material.

[0068] (3) Low-temperature fermentation again: The temperature is controlled at 38°C by the heat recovery effect of the matrix rod. 0.5L of mixed bacterial solution composed of black soil inducing bacteria and low-temperature fermentation bacteria in a ratio of 30% and 70% is injected into the high-temperature fermentation material. The bacterial solution concentration is 10^8 cfu / ml. Low-temperature fermentation is carried out for 72 hours. The material temperature is adjusted to 28°C again by the heat recovery effect of the matrix rod and then put into use.

[0069] (4) Dual-biological culture: 0.5L of a mixed bacterial solution consisting of black soil inducing bacteria and low-temperature fermentation bacteria at a ratio of 20% and 80% was injected into the material obtained in step (3) using a multifunctional matrix rod fermentation system. The bacterial solution concentration was 10^8 cfu / ml. At the same time, 20 cubic meters of fresh air were injected. The material was then transferred to a culture bed, and 10% of the total weight of Eisenia fetida and Heterolipa worms were added. Under the action of the dual-biological system, the material was cultured at 15-28℃ for 7-10 days. The earthworms were then separated using a photosensitive earthworm-interactive separation and harvesting device to obtain regenerated black soil. The equipment used in this embodiment is the same as in embodiment 1.

[0070] The effective components of the twin black soil prepared in Example 3 were determined, and the experimental results are as follows: the organic matter content was 380 g / kg, the total nitrogen was 1.426%, the total phosphorus was 2.71 g / kg, and the total potassium was 1.52%.

[0071] According to the soil nutrient grading standards of the Second National Soil Survey, soil with organic matter >4% meets the standard for Grade I soil. As shown in Examples 2 and 3, the organic matter content of the regenerated black soil prepared by the dual-biological method of this invention is 2.9-9.5 times higher than the minimum standard for Grade I soil.

[0072] The various embodiments in the specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0073] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a twin black soil, characterized by, It comprises the following steps: (1) low-temperature fermentation: plant waste is crushed and added to a multifunctional matrix rod fermentation system, black soil inducing bacteria and low-temperature fermentation bacteria are injected, the fermentation temperature is controlled in the range of 35-40℃ through the heat recovery effect of the matrix rod, low-temperature fermentation is carried out for 72h, and low-temperature fermented material is obtained; (2) high-temperature fermentation: high-temperature fermentation bacteria and air are injected into the low-temperature fermented material through the multifunctional matrix rod fermentation system, the temperature is controlled at 70-75℃, high-temperature fermentation is carried out for 24h, and high-temperature fermented material is obtained; (3) low-temperature fermentation again: the temperature is adjusted to 35-40℃ through the heat recovery effect of the matrix rod, black soil inducing bacteria and low-temperature fermentation bacteria are injected into the obtained high-temperature fermented material, low-temperature fermentation is carried out for 72h, the material temperature is adjusted to 28℃ again through the heat recovery effect of the matrix rod, and standby is prepared; (4) double-biological culture: after black soil inducing bacteria, low-temperature fermentation bacteria and air are injected into the material obtained in step (3) through the multifunctional matrix rod fermentation system, the material is transferred to a culture bed, low-temperature resistant and saprobic earthworms are put in, under the action of double organisms, the material is cultured at 15-28℃ for 7-10d, the earthworms and soil are separated, and regenerated black soil is obtained. The black soil inducing bacteria in steps (1), (3) and (4) are composed of 35% Aspergillus niger and 65% Bacillus subtilis; the low-temperature fermentation bacteria are composed of 25% Rhizobium, 45% Acetobacter xylinum, 20% Lactobacillus and 10% Methanosarcina.

2. The method for preparing the twin black soil according to claim 1, characterized in that, The plant waste in step (1) has a particle size of 100-200 mesh; the black soil inducing bacteria and low-temperature fermentation bacteria are added in a ratio of 20% and 80% respectively; 0.5L of mixed bacteria liquid of black soil inducing bacteria and low-temperature fermentation bacteria is added per ton of plant waste with a humidity of 40%; the concentration of the mixed bacteria liquid is 10 8 cfu / ml.

3. The method of claim 1, wherein the twin black soil is prepared by the steps of: The high-temperature fermentation bacteria in step (2) include thermophilic bacteria and / or high-temperature actinomycetes, and the addition amount of the high-temperature fermentation bacteria is 0.5 L of high-temperature fermentation bacteria liquid per ton of material calculated at 40% humidity, and the concentration of the high-temperature fermentation bacteria liquid is 10 8 cfu / ml; and the addition amount of air is 20 cubic meters of fresh air per ton of material calculated at 40% humidity.

4. The method of claim 1, wherein the twin black soil is prepared by the steps of: The adding ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria in step (3) is 30% and 70% respectively, and 0.5L of mixed bacteria liquid of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton of material with 40% humidity, wherein the concentration of the mixed bacteria liquid is 10 8 cfu / ml.

5. The method of claim 1, wherein the twin black soil is prepared by the steps of: The adding ratio of the black soil inducing bacteria and the low-temperature fermentation bacteria in step (4) is 20% and 80% respectively, and 0.5 L of mixed bacteria liquid of the black soil inducing bacteria and the low-temperature fermentation bacteria is added per ton of material with 40% humidity, wherein the concentration of the mixed bacteria liquid is 10 8 cfu / ml.

6. The method for preparing the twin black soil according to claim 1, characterized in that, The amount of low-temperature resistant and saprobic earthworms put in in step (4) is not less than 10% of the total weight of the material; the amount of air added is 20 cubic meters of fresh air per ton of material with 40% humidity.

7. The use of the method according to any one of claims 1-6 in the preparation of double black soil.

8. Double black soil prepared by the method according to any one of claims 1-6.

9. The use of the double black soil according to claim 8 in soil improvement and crop cultivation.

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