Instant recombinant collagen nanofiber mask as well as preparation method and application thereof

Abstract

The invention discloses an instant recombinant collagen nanofiber mask as well as a preparation method and application thereof. The nanofiber facial mask in the facial mask is a layered substance formed by continuously stacking fibers by taking small-molecular-weight recombinant collagen, high-molecular-weight recombinant collagen and water-soluble polymers as matrixes and utilizing an electrostatic spinning technology, and has the advantages of large specific surface area, high porosity, high active substance loading rate and the like; the active ingredients are attached or embedded into the nanofibers in the form of small particles, so that the absorption efficiency is higher, and the active ingredients can easily permeate into the deep layer of the skin, so that the functions of moisturizing, resisting oxidation, resisting inflammation, resisting aging, whitening, relieving and the like are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of fiber membranes, and in particular to a quick-dissolving recombinant collagen nanofiber facial mask and a preparation method and application thereof. Background Art

[0002] In the cosmetics market, facial masks are important functional cosmetics for personal care. 70% of the facial masks in the market are ordinary sheet masks with non-woven fabric as the backing cloth. There are the following problems when using them: (1) The design of non-woven masks is not good enough to fit the facial curve or the size is not suitable, resulting in poor mask effect; (2) The essence is unevenly distributed and cannot be completely absorbed by the skin, resulting in ingredient waste; (3) The active substances in wet masks are easy to deteriorate, have a short shelf life, and contain preservatives that may cause allergies; (4) It is inconvenient to use and takes a long time, ranging from 15 to 20 minutes, and the skin care efficiency is low. With the growth of national income, the requirements for self-image continue to increase. Consumers not only hope that facial mask products have better absorption performance, but also have further requirements for the safety performance of products. New functions such as simple use steps, convenience, personalized use, and no added preservatives have become the focus of innovation in facial mask care products. Solid instant masks can solve the above problems to a certain extent and meet consumer needs. They are safe and do not require the addition of preservatives. They are simple to use, dissolve quickly in water, and can be personalized according to skin needs. They have broad application prospects.

[0003] At present, the preparation of solid instant facial masks can be carried out by electrospinning, facial mask liquid drying and curing, etc. Among them, electrospinning technology is a new type of nanofiber manufacturing technology. The conventional electrospinning device is mainly composed of a high-voltage power supply, a propulsion pump, a nozzle and a receiver. This technology uses the electric field force to form a jet of charged polymer solution or melt, and finally forms nanofibers as the solvent evaporates and deposits into a fiber film at the end of the receiving device. By adjusting process parameters such as voltage, liquid propulsion speed, collection distance, etc., the fiber diameter and microscopic morphology can be effectively controlled. Compared with other preparation methods, the electrospinning method has the advantages of simple equipment, low cost, and easy control of fiber morphology. The mask based on nanofibers has a huge specific surface area and can absorb more active ingredients, making it have a high loading rate and far superior to other mask carriers. The active ingredients are attached or embedded in the nanofibers in the form of tiny particles, have strong mutual penetration with other substances, and have higher absorption efficiency. This also provides a direction for the research and development of solid nanofiber masks, realizing nano-level skin care and launching high-efficiency products.

[0004] Chinese patent application CN202010797121.9 (publication date: 2020-10-27) discloses a chitosan-human recombinant collagen electrospinning nanofiber scaffold and a preparation method. In this method, hexafluoroisopropanol is used as the electrospinning liquid solvent, which has certain toxicity and is corrosive to the skin when it comes into contact with the skin when it is not completely volatilized, increasing the difficulty of production and application.

[0005] Chinese patent application CN202310672941.9 (publication date: 2023-08-29) discloses a method for preparing a recombinant collagen nano instant mask using electrospinning. The main components of the mask prepared by this patent are yeast recombinant collagen, sodium hyaluronate, budding stalk enzyme polysaccharide and surfactant. The average molecular weight of the yeast recombinant collagen used is between 20,000-100,000, the average molecular weight of sodium hyaluronate is between 20,000-60,000, and the molecular weight of budding stalk enzyme polysaccharide is between 100,000-300,000. The penetration effect is low. Most of the recombinant collagen, sodium hyaluronate, and budding stalk enzyme polysaccharide will stay on the surface of the skin, thereby hindering the penetration of other active ingredients, resulting in poor overall product effect. In addition, in order to improve the toughness of the spinning membrane, 0.1-4 parts by weight of high molecular weight polyethylene glycol is added so that the product is not easily destroyed by external forces during storage and transportation. Although polyethylene glycol is widely used in skin care products due to its good moisturizing, permeability and stability, it may also cause allergies as a sensitizing ingredient. In addition, polyethylene glycol may reduce the skin barrier function and increase the skin's sensitivity to external stimuli. Moreover, people with sensitive skin should use 0.001-2 parts by weight of functional substances, such as preservatives, with caution.

[0006] Therefore, providing a product with stable properties, better effects, safer and gentler ingredients will help meet consumers' needs in daily care. Summary of the invention

[0007] The collagen nano instant facial mask of recombinant collagen in the prior art has poor penetration and absorption effect. The high molecular weight recombinant collagen will stay on the skin surface, thereby hindering the penetration of other active ingredients, resulting in poor product absorption effect. In order to solve the problems existing in the prior art, the primary purpose of the present invention is to provide a method for preparing an instant recombinant collagen nanofiber facial mask.

[0008] Another object of the present invention is to provide a fast-dissolving recombinant collagen nanofiber mask obtained by the above-mentioned preparation method.

[0009] Another object of the present invention is to provide an application of the instant recombinant collagen nanofiber mask.

[0010] The purpose of the present invention is achieved through the following technical solutions: A method for preparing a quick-dissolving recombinant collagen nanofiber facial mask comprises the following steps: S1. Use sterile water as solvent, add 0.1-15wt.% of low molecular weight recombinant collagen, 0.1-25wt.% of high molecular weight recombinant collagen, and 1-30wt.% of water-soluble polymer, and stir evenly in a constant temperature water bath at 20°C to 60°C; after the solution is cooled to room temperature, add 0.1-10wt.% of active ingredient, 0.1-5wt.% of emollient, and 0.1-1wt.% of thickener, and stir evenly at room temperature until the solution is uniform and free of particles to obtain a spinning solution; S2, selecting a receiving substrate, using the spinning solution obtained in step S1, spinning at a temperature of 20-40°C and a humidity of 30%-40%, and controlling the spinning conditions: the spinning voltage is 10-40 kV, the automatic liquid supply speed is 0.2-2 mL / h, the reel collection speed is 100-150 rpm, and the moving speed of the electrospinning equipment is set to 15-25 mm / s; S3. Eliminate static electricity on the spinning product obtained in step S2 and dry and solidify it to obtain the instant recombinant collagen nanofiber mask; wherein the mass fraction of the recombinant collagen in the nanofiber mask after solidification is greater than 30%.

[0011] Furthermore, in step S1, the recombinant collagen is recombinant human type III collagen; further preferably, it is recombinant human type III collagen obtained by methanol-induced Pichia pastoris fermentation; wherein, the average molecular weight of small molecular weight recombinant collagen is 2000-6000Da, and the average molecular weight of large molecular weight recombinant collagen is 20000-70000Da; further preferably, the average molecular weight of small molecular weight recombinant collagen is 4000-6000Da, and the average molecular weight of large molecular weight recombinant collagen is 30000-50000Da; most preferably, the average molecular weight of small molecular weight recombinant collagen is 5000Da, and the average molecular weight of large molecular weight recombinant collagen is 33000Da.

[0012] Furthermore, in step S1, the water-soluble polymer includes any one or more combinations of pullulan, polyvinyl alcohol, polyethylene oxide, polyvinyl pyrrolidone, sodium alginate, chitosan, carboxymethyl chitosan, starch, carboxymethyl cellulose, gelatin, hyaluronic acid and sodium hyaluronate.

[0013] Further, in step S1, the active ingredients include niacinamide, arbutin, dipotassium glycyrrhizinate, glabridin, purslane extract, chamomile extract, roxburghii extract, Centella asiatica extract, honeysuckle extract, salvia miltiorrhiza extract, glycerin, urea capsule, betaine, squalane,β - Any one or more combinations of glucan, ceramide NP, panthenol, acetyl glucosamine, chitosan, tocopherol, and tocopherol acetate. The active ingredients and recombinant collagen have complementary effects. The ingredients are natural, mild, and have moisturizing, soothing, anti-oxidation, anti-inflammatory and other effects. They can be used by people with sensitive skin and other susceptible skin. By selecting different base materials and water-soluble polymers, the needs of different skin types can be met, and nanofiber masks of various bases can be produced.

[0014] Further, in step S1, the emollient includes any one or more combinations of soybean lecithin, ethylhexylglycerin, triethylhexanoin, polydimethylsiloxane, bis-PEG-18 methyl ether dimethyl silane, caprylic / capric triglyceride, bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate, polyglyceryl-10 myristate, polyglyceryl-10 laurate, C14-22 alcohol, and C18-21 alkane. By mixing one or more emollients, the solid instant mask can be given a more lubricated feel and the stickiness caused by the nanofiber skeleton can be reduced.

[0015] Furthermore, in step S1, the thickener component includes any one or more combinations of carbomer, xanthan gum, sclerotium gum, guar gum, carageenan, methylcellulose, hydroxypropyl methylcellulose, and sodium carboxymethyl starch. The thickener imparts moderate viscosity to the electrospinning solution, which is beneficial to electrospinning and makes the obtained nanofiber layer have a good morphology.

[0016] Furthermore, in step S1, sterile water is used as a solvent, 10 to 15 wt.% of a low molecular weight recombinant collagen, 15 to 20 wt.% of a high molecular weight recombinant collagen, and 5 to 15 wt.% of a water-soluble polymer are added, and the mixture is stirred evenly in a constant temperature water bath at 20°C to 60°C; after the solution is cooled to room temperature, 3 to 8 wt.% of an active ingredient, 0.5 to 2 wt.% of an emollient, and 0.1 to 0.5 wt.% of a thickener are added, and the mixture is stirred evenly at room temperature until the solution is uniform and free of particles to obtain a spinning solution.

[0017] Furthermore, in step S2, the substrate includes any one or more combinations of non-woven fabric, snowflake mesh fabric, meltblown fabric, release film, silicone oil paper and hot pressed fabric.

[0018] Furthermore, in step S2, the spinning solution obtained in step S1 is used to carry out spinning at a temperature of 25 to 35°C and a humidity of 35% to 45%, and the spinning conditions are controlled as follows: the spinning voltage is 20 to 35 kV, the automatic liquid supply speed is 0.5 to 0.8 mL / h, the reel collection speed is 110 to 130 rpm, and the moving speed of the electrospinning equipment is set to 18 to 23 mm / s; further preferably, spinning is carried out at a temperature of 30°C and a humidity of 40%, and the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / h, the reel collection speed is 120 rpm, and the moving speed of the electrospinning equipment is set to 20 mm / s.

[0019] A method for preparing a quick-dissolving recombinant collagen nanofiber facial mask is obtained by the above-mentioned preparation method.

[0020] The instant recombinant collagen nanofiber mask is used in the preparation of moisturizing, anti-oxidation, anti-inflammatory, anti-aging, whitening and / or soothing mask products.

[0021] The recombinant collagen used in the present invention is a fermentation product derived from yeast. Compared with collagen from animal sources, it has good biological activity, and has high sequence similarity and good affinity with human collagen. It has the effects of repairing skin barrier, promoting cell growth, removing wrinkles and moisturizing, and has no immunogenicity. By using a ratio of low molecular weight recombinant collagen and high molecular weight recombinant collagen, the skeleton structure of the nanofiber mask can be improved, making the nanofiber mask light and thin, easily soluble in water, large molecular weight recombinant collagen and other skin care ingredients stay on the skin surface to form a skin protective film, and small molecular weight recombinant collagen penetrates into the deep skin.

[0022] Compared with the prior art, the present invention has the following advantages and effects: The nanofiber mask in the mask of the present invention uses low molecular weight recombinant collagen, high molecular weight recombinant collagen, and water-soluble polymer as a matrix, and uses electrostatic spinning technology to manufacture a layered object formed by continuous fiber accumulation, which has the advantages of large specific surface area, high porosity, and high active substance loading rate, and the active ingredients are attached or embedded in the nanofibers in the form of tiny particles, and the absorption efficiency is higher, and it is easy to penetrate the deep layer of the skin, thereby playing the functions of moisturizing, anti-oxidation, anti-aging, whitening, and soothing. Since the mask of the present invention is a solid dry mask, no preservative ingredients need to be added, and the formula ingredients are more gentle and safe. In addition, it also has the characteristics of portability and simple and convenient use. When used, it only needs to moisten the skin with ordinary moisturizing spray products or clean the face. If there is water left, peel off the nanofiber layer and apply it to various parts of the face according to demand, such as as a mask, eye mask, forehead and neck patch, etc., which will melt when exposed to water, and the skin will be evenly distributed after patting the skin. No need to wash after use, it can keep the skin moist and firm.

[0023] The experimental results of scanning electron microscopy confirm that the nanofiber layer of the facial mask of the present invention is very uniform, without any defects such as clumping, nodes, broken fibers, and voids, thereby ensuring uniform distribution of active substances, high loading rate, and close fit to the skin, ensuring that the formed fiber structure is close to the arrangement of skin cells, and is more conducive to fully exerting the care effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The invention provides a flowchart of the steps of the instant recombinant collagen nanofiber mask and its preparation method. DETAILED DESCRIPTION

[0025] The present invention provides a method for preparing an instant recombinant nanofiber facial mask, which comprises the following specific steps: (1) Preparation of spinning solution: using sterile water as solvent, adding 0.1-15wt.% of low molecular weight recombinant collagen, 0.1-25wt.% of high molecular weight recombinant collagen, and 1-30wt.% of water-soluble polymer, and stirring uniformly, the stirring temperature is 20-60°C; (2) cooling the spinning solution of (1) to room temperature, adding 0.1 to 10 wt.% of active ingredient, 0.1 to 5 wt.% of emollient, and 0.1 to 1 wt.% of thickener, and stirring evenly at room temperature; (3) selecting a receiving substrate, wherein the substrate includes one or a combination of non-woven fabric, snowflake mesh fabric, release film, silicone oil paper and hot pressed fabric; (4) using the receiving substrate in (3) and the spinning solution prepared in (2), electrospinning is performed in an environment with a temperature of 25 to 40° C. and a humidity of 30 to 50%; (5) The product obtained in (4) is subjected to static elimination, and vacuum drying and solidification to obtain a solid nanofiber mask, wherein the mass fraction of the recombinant collagen in the nanofiber mask after solidification is greater than 30%.

[0026] The present invention is further described in detail below through specific examples.

[0027] The recombinant collagen used in the following examples or comparative examples is a product obtained by methanol-induced Pichia yeast fermentation, wherein the average molecular weight of the small molecular weight recombinant collagen is 5000Da, and the average molecular weight of the large molecular weight recombinant collagen is 33000Da. The above-mentioned recombinant collagen is mainly rich in amino acids such as glycine, proline, alanine, glutamic acid, lysine, arginine, aspartic acid, and serine, which meets the high repetition of the Gly-XY sequence of the collagen domain of natural collagen, and is mainly classified as type III human recombinant collagen. Recombinant collagen has good biocompatibility, high activity, no endotoxin, rejection reaction, etc. Among them, recombinant collagen with a smaller molecular weight is easily absorbed by the skin, can moisturize the skin, supplement the amino acids required by the skin, repair damaged skin, and restore skin elasticity; recombinant collagen with a larger molecular weight can be used as a nanofiber skeleton component to load recombinant collagen with smaller molecules and active substances.

[0028] Example 1 Receiving substrate: The release film is used as the receiving substrate of the nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 10wt.% of low molecular weight recombinant collagen, 10wt.% of high molecular weight recombinant collagen, 8wt.% of polyvinyl alcohol, and 4wt.% of pullulan, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% of betaine, 1wt.% of squalane, 0.5wt.% of polydimethylsiloxane, and 0.1wt.% of xanthan gum, stirring evenly at room temperature until the solution is uniform and free of particles, thereby obtaining a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, thereby obtaining a solid instant recombinant collagen nanofiber mask. The obtained product has the effect of moisturizing.

[0029] Comparative Example 1 Receiving substrate: The release film is used as the receiving substrate of the nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 15wt.% low molecular weight recombinant collagen, 8wt.% pullulan, and 2wt.% polyvinyl alcohol, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% betaine, 1wt.% squalane, 0.5wt.% polydimethylsiloxane, and 0.1wt.% xanthan gum, stirring evenly at room temperature until the solution is uniform and free of particles, thereby obtaining a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The nanofiber membrane prepared in this comparative example cannot identify the nanofiber morphology. It can be seen that it is difficult to prepare a nanofiber membrane with good morphology from a single small molecule recombinant collagen.

[0030] Comparative Example 2 Receiving substrate: The release film is used as the receiving substrate of the nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 30wt.% low molecular weight recombinant collagen, 5wt.% pullulan, and 5wt.% polyvinyl alcohol, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% betaine, 1wt.% squalane, 0.5wt.% polydimethylsiloxane, and 0.1wt.% xanthan gum thickener, stirring evenly at room temperature until the solution is uniform and free of particles, thereby obtaining a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The nanofiber membrane prepared in this comparative example has defects such as agglomeration, nodes, and beaded structure. It can be seen that it is difficult to prepare a nanofiber membrane with good morphology from a single small molecule recombinant collagen.

[0031] Comparative Example 3 Receiving substrate: Silicone oil paper is used as the receiving substrate of nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 15wt.% high molecular weight recombinant collagen, 5wt.% pullulan, and 5wt.% polyvinyl alcohol, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% betaine, 1wt.% squalane, 0.5wt.% polydimethylsiloxane, and 0.1wt.% xanthan gum thickener, stirring evenly at room temperature until the solution is uniform and free of particles, thereby obtaining a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The nanofiber membrane prepared in this comparative example has defects such as agglomeration, nodes, broken fibers, voids, and beaded structures. It can be seen that it is difficult to prepare a nanofiber membrane with good morphology by preparing a spinning solution from a single macromolecular recombinant collagen at this concentration.

[0032] Example 2 Receiving substrate: Silicone oil paper is used as the receiving substrate of nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 15wt.% of low molecular weight recombinant collagen, 15wt.% of high molecular weight recombinant collagen, 6wt.% of polyvinyl alcohol, 4wt.% of polyethylene oxide, and 2wt.% of pullulan, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% of betaine, 2wt.% of purslane extract, 1wt.% of squalane, 0.5wt.% of polydimethylsiloxane, and 0.1wt.% of xanthan gum thickener, stirring evenly at room temperature until the solution is uniform and free of particles, to obtain a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The obtained product has the effect of moisturizing and soothing. In this embodiment, the spinning solution is prepared by compounding macromolecular and small molecule recombinant collagen, and a nanofiber membrane without any defects such as agglomeration, node, broken wire, and void can be obtained, with good morphology and uniform thickness of nanofibers.

[0033] Example 3 Receiving substrate: meltblown cloth is used as the receiving substrate of nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 10wt.% low molecular weight recombinant collagen, 20wt.% high molecular weight recombinant collagen, 6wt.% polyethylene oxide, 4wt.% polyvinyl alcohol, 2wt.% pullulan, and stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% betaine, 1wt.% roxburghii extract, 1wt.% squalane, 0.5wt.% polydimethylsiloxane, and 0.1wt.% xanthan gum thickener, and stirring evenly at room temperature until the solution is uniform and free of particles, to obtain a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the nanofiber mask is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The obtained product has the effect of moisturizing and anti-oxidation. In this embodiment, the spinning solution is prepared by compounding macromolecular and small molecule recombinant collagen, and a nanofiber membrane without any defects such as agglomeration, node, broken wire, and void can be obtained, with good morphology and uniform thickness of nanofibers.

[0034] Example 4 Receiving substrate: Snowflake mesh pressed cloth is used as the receiving substrate of nanofibers; Preparation of spinning solution: using sterile water as solvent, adding 20wt.% of low molecular weight recombinant collagen, 10wt.% of high molecular weight recombinant collagen, 5wt.% of pullulan, 4wt.% of polyvinyl alcohol, and 3wt.% of polyethylene oxide, stirring evenly in a constant temperature water bath at 60°C; after the solution is cooled to room temperature, adding 2wt.% of betaine, 0.5wt.% of β-glucan, 0.1wt.% of ceramide NP, 0.5wt.% of polydimethylsiloxane, 0.1wt.% of soybean lecithin, and 0.1wt.% of xanthan gum thickener, stirring evenly at room temperature until the solution is uniform and free of particles, to obtain a spinning solution; Electrospinning: Using the above-mentioned receiving substrate and spinning solution, the spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / L, the reel collection speed is 120 rpm, the moving speed of the electrospinning equipment is set to 20 mm / s, and the spinning is carried out at a temperature of 30°C and a humidity of 40%; The static electricity is eliminated and the mixture is dried and solidified, so that the mass fraction of the recombinant collagen in the nanofiber mask after solidification is more than 30%, and a solid instant recombinant collagen nanofiber mask is obtained. The obtained product has the effect of moisturizing and repairing. In this embodiment, the spinning solution is prepared by compounding macromolecular and small molecule recombinant collagen, and a nanofiber membrane without any defects such as agglomeration, node, broken wire, and void can be obtained, with good morphology and uniform thickness of nanofibers.

[0035] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the described embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention shall be equivalent replacement methods and shall be included in the protection scope of the present invention.

Claims

1. A method for preparing an instant recombinant collagen nanofiber facial mask, characterized in that: The steps include: S1. Use sterile water as solvent, add 0.1-15wt.% of low molecular weight recombinant collagen, 0.1-25wt.% of high molecular weight recombinant collagen, and 1-30wt.% of water-soluble polymer, and stir evenly in a constant temperature water bath at 20°C to 60°C; after the solution is cooled to room temperature, add 0.1-10wt.% of active ingredient, 0.1-5wt.% of emollient, and 0.1-1wt.% of thickener, and stir evenly at room temperature until the solution is uniform and free of particles to obtain a spinning solution; S2, selecting a receiving substrate, using the spinning solution obtained in step S1, spinning at a temperature of 20-40°C and a humidity of 30%-50%, and controlling the spinning conditions: the spinning voltage is 10-40 kV, the automatic liquid supply speed is 0.2-1 mL / h, the reel collection speed is 100-150 rpm, and the moving speed of the electrospinning equipment is set to 15-25 mm / s; S3, eliminating static electricity from the spinning product obtained in step S2 and drying and curing it, so as to obtain the instant recombinant collagen nanofiber mask.

2. The method for preparing the instant recombinant collagen nanofiber mask according to claim 1, characterized in that: In step S1, the recombinant collagen is recombinant type III human collagen; wherein the average molecular weight of the small molecular weight recombinant collagen is 2000-6000 Da, and the average molecular weight of the large molecular weight recombinant collagen is 20000-70000 Da.

3. The method for preparing the instant recombinant collagen nanofiber facial mask according to claim 1, characterized in that: In step S1, the recombinant collagen is obtained by methanol-induced Pichia pastoris fermentation; wherein the average molecular weight of the small molecular weight recombinant collagen is 4000-6000 Da, and the average molecular weight of the large molecular weight recombinant collagen is 30000-50000 Da.

4. The method for preparing the instant recombinant collagen nanofiber mask according to claim 1, characterized in that: In step S1, the water-soluble polymer includes any one or more combinations of pullulan, polyvinyl alcohol, polyethylene oxide, polyvinyl pyrrolidone, sodium alginate, chitosan, carboxymethyl chitosan, starch, carboxymethyl cellulose, gelatin, hyaluronic acid and sodium hyaluronate; In step S1, the active ingredients include niacinamide, arbutin, dipotassium glycyrrhizinate, glycyrrhizin, purslane extract, chamomile extract, roxburghii extract, centella asiatica extract, honeysuckle extract, salvia miltiorrhiza extract, glycerin, urea capsule, betaine, squalane, β - Any one or more combinations of dextran, ceramide NP, panthenol, acetyl glucosamine, chitosan, tocopherol, and tocopherol acetate.

5. The method for preparing the instant recombinant collagen nanofiber facial mask according to claim 1, characterized in that: In step S1, the emollient includes any one or more combinations of soy lecithin, ethylhexylglycerin, triethylhexanoin, polydimethylsiloxane, bis-PEG-18 methyl ether dimethyl silane, caprylic / capric triglyceride, bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate, polyglyceryl-10 myristate, polyglyceryl-10 laurate, C14-22 alcohol, and C18-21 alkane; In step S1, the thickener component includes any one or more combinations of carbomer, xanthan gum, sclerotium gum, guar gum, carrageenan, methyl cellulose, hydroxypropyl methyl cellulose, and sodium carboxymethyl starch; In step S2, the substrate includes any one or more combinations of non-woven fabric, snowflake mesh fabric, meltblown fabric, release film, silicone oil paper and hot pressed fabric.

6. The method for preparing the instant recombinant collagen nanofiber mask according to claim 1, characterized in that: In step S1, sterile water is used as a solvent, 10 to 15 wt.% of low molecular weight recombinant collagen, 15 to 20 wt.% of high molecular weight recombinant collagen, and 5 to 15 wt.% of a water-soluble polymer are added, and the mixture is stirred evenly in a constant temperature water bath at 20°C to 60°C; after the solution is cooled to room temperature, 3 to 8 wt.% of an active ingredient, 0.5 to 2 wt.% of an emollient, and 0.1 to 0.5 wt.% of a thickener are added, and the mixture is stirred evenly at room temperature until the solution is uniform and free of particles, thereby obtaining a spinning solution.

7. The method for preparing the instant recombinant collagen nanofiber facial mask according to claim 1, characterized in that: In step S2, the spinning solution obtained in step S1 is used to carry out spinning at a temperature of 25 to 35°C and a humidity of 35% to 45%. The spinning conditions are controlled as follows: the spinning voltage is 20 to 35 kV, the automatic liquid supply speed is 0.5 to 0.8 mL / h, the reel collection speed is 110 to 130 rpm, and the moving speed of the electrospinning equipment is set to 18 to 23 mm / s.

8. The method for preparing the instant recombinant collagen nanofiber facial mask according to claim 1, characterized in that: In step S2, the spinning solution obtained in step S1 is used to perform spinning at a temperature of 30°C and a humidity of 40%. The spinning conditions are controlled as follows: the spinning voltage is 25 kV, the automatic liquid supply speed is 0.5 mL / h, the reel collection speed is 120 rpm, and the moving speed of the electrospinning equipment is set to 20 mm / s.

9. A method for preparing a fast-dissolving recombinant collagen nanofiber facial mask, characterized in that: The method is obtained by the preparation method described in any one of claims 1 to 8.

10. Use of the instant recombinant collagen nanofiber mask described in claim 9 in the preparation of moisturizing, anti-oxidation, anti-aging, whitening and / or soothing facial mask products.

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