A repair and anti-aging composition specially developed for dry and sensitive skin and its application

Abstract

This disclosure provides a repair and anti-aging composition specifically formulated for dry and sensitive skin, and its application, belonging to the field of cosmetic technology. The disclosure selects a specific mass ratio of inositol, ceramide NP, phytosphingosine, golden camellia extract, bayberry bark extract, and cnidium monnieri extract for the anti-aging composition. These six components, when used together, form a powerful anti-aging network, acting in multiple ways to enhance the anti-aging composition's penetration-promoting, repairing, moisturizing, anti-aging, and soothing effects.

Description

Technical Field

[0001] The present disclosure relates to the technical field of cosmetics, and in particular to a repairing and anti-aging composition specially developed for dry and sensitive skin and its application. Background Art

[0002] Dry, sensitive skin is a common skin type characterized by dryness, itching, redness, swelling, and hypersensitivity to external stimuli. This skin type's characteristics primarily stem from a damaged epidermal barrier, leading to water loss and a decreased ability to resist external stimuli. Due to insufficient moisture and a damaged barrier, dry, sensitive skin is more susceptible to aging symptoms such as fine lines, wrinkles, and sagging, increasing the urgent demand for anti-aging products.

[0003] Among existing technologies, many anti-aging products focus on using highly active ingredients such as retinol, peptides, and antioxidants to promote skin cell regeneration and reduce wrinkles. However, many of these ingredients can irritate dry and sensitive skin, causing redness, swelling, or burning. Traditional moisturizing ingredients such as glycerin and hyaluronic acid, while effective at moisturizing, have limited effects on deep skin repair. Furthermore, many existing products contain ingredients such as fragrances and alcohol, which can exacerbate skin irritation and dryness. Therefore, consumers with dry and sensitive skin often face a dilemma when using anti-aging products: effectiveness versus safety. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and to provide a repair and anti-aging composition specifically designed for dry and sensitive skin and its application.

[0005] To achieve the above-mentioned objectives, the technical solution adopted by the present invention is as follows: in the first aspect, an anti-aging composition is provided, which comprises the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of golden camellia extract, 0.1-5 parts of bayberry bark extract, and 0.1-5 parts of cnidium monnieri extract.

[0006] In some embodiments, the anti-aging composition comprises the following components in parts by weight: 0.5-2 parts of inositol, 0.05-0.5 parts of ceramide-NP, 0.05-0.5 parts of phytosphingosine, 0.5-3 parts of camellia chrysantha extract, 0.5-3 parts of bayberry bark extract, and 1-4 parts of cnidium monnieri extract.

[0007] In some embodiments, the anti-aging composition comprises the following components in parts by weight: 1-1.5 parts of inositol, 0.1-0.2 parts of ceramide-NP, 0.2-0.3 parts of phytosphingosine, 1-2 parts of camellia chrysantha extract, 1-2 parts of bayberry bark extract, and 2-3 parts of cnidium monnieri extract.

[0008] In some embodiments, in the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%.

[0009] In some embodiments, the mass percentage of myricitrin in the bayberry bark extract is 5-15%; and / or the mass percentage of osthole in the cnidium monnieri extract is 2-10%.

[0010] In some embodiments, the method for preparing the Camellia chrysantha extract comprises the following steps:

[0011] The flowers of Camellia chrysantha are dried and crushed, and passed through an 80-150 mesh sieve to obtain Camellia chrysantha powder;

[0012] The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL.

[0013] In some embodiments, the volume fraction of the ethanol solution is 60-90%; and / or the extraction is ultrasonic extraction, the ultrasonic extraction temperature is 30-40°C, the ultrasonic extraction time is 20-50 min, and the ultrasonic extraction power is 80-200W.

[0014] In a second aspect, the invention provides the use of the anti-aging composition in the preparation of cosmetics.

[0015] In a third aspect, a cosmetic is provided, comprising the anti-aging composition, wherein the content of the anti-aging composition in the cosmetic is 1-20 wt %.

[0016] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH regulator, and the balance water.

[0017] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0018] And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0019] and / or, the pH regulator comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0020] and / or, the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0021] And / or, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: the anti-aging composition disclosed herein selects inositol, ceramide-NP, phytosphingosine, golden camellia extract, bayberry bark extract and cnidium monnieri extract in a specific mass ratio. The combined use of these six components can form a powerful anti-aging network, which works together from multiple aspects to improve the penetration-promoting, repairing, moisturizing, anti-aging and soothing effects of the anti-aging composition. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a comparison of eye wrinkles before and after 28 days of use in the blank application example and application example 1 in effect example 4. DETAILED DESCRIPTION

[0024] To facilitate understanding of the present disclosure, a more comprehensive description will be given below. However, the present disclosure can be implemented in many different forms and is not limited to the embodiments described herein. Rather, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of the present disclosure.

[0025] As used herein:

[0026] "Prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variations thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0027] The conjunction "consisting of" excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed, excluding materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim, rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0028] When amount, concentration or other value or parameter are represented with range, preferred range or the range that a series of upper preferred value and lower preferred value limit are expressed, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, and no matter whether this scope is disclosed separately.For example, when disclosing scope " 1-5 ", described scope should be interpreted as including scope " 1-4 ", " 1-3 ", " 1-2 ", " 1-2 and 4-5 ", " 1-3 and 5 " etc.When numerical range is described in this article, unless otherwise stated, otherwise this scope is intended to include its end value and all integers and fractions within this range.

[0029] In these examples, parts and percentages are by mass unless otherwise indicated.

[0030] "Parts by mass" refers to the basic unit of measurement used to express the mass ratio of multiple components. One part can represent any unit of mass, such as 1g or 2.689g. If we say that the mass of component A is a parts and the mass of component B is b parts, this means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, we could say that the mass of component A is aK and the mass of component B is bK (K is an arbitrary number representing a multiplication factor). It's important to note that, unlike parts by mass, the sum of the mass of all components is not limited to 100 parts.

[0031] "And / or" is used to indicate that one or both of the stated situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0032] In order to solve the dilemma between the irritation and anti-aging effect of anti-aging compositions for dry and sensitive skin.

[0033] In a first aspect of the present disclosure, an anti-aging composition is provided, comprising the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of camellia chrysanthemi extract, 0.1-5 parts of myrica rubra bark extract, and 0.1-5 parts of cnidium monnieri extract.

[0034] The anti-aging composition disclosed herein selects inositol, ceramide-NP, phytosphingosine, golden camellia extract, bayberry bark extract and cnidium monnieri extract in a specific mass ratio. The six components are used in combination to form a powerful anti-aging network, which works together from multiple aspects to improve the penetration-promoting, repairing, moisturizing, anti-aging and soothing effects of the anti-aging composition. Among them, inositol, ceramide-NP and phytosphingosine are added to target the key lipid components lacking in dry and sensitive skin. Inositol is a precursor of phosphatidylinositol and its metabolites in human skin, which helps to repair the damaged skin lipid layer, increase the skin's moisturizing ability, promote the healthy growth of skin cells, and delay the aging process; ceramide-NP is an important skin barrier component that can directly repair the skin barrier damaged by dehydration, reduce the damage to the skin caused by external stimuli, relieve discomfort symptoms such as dryness and redness, and has anti-aging effects, reducing the appearance of fine lines and wrinkles; phytosphingosine keeps the skin soft and smooth by enhancing the skin's own defense capabilities; while inositol, ceramide-NP and phytosphingosine have low absorption rates in dry and sensitive skin, the present disclosure found that adding Camellia chrysantha extract to the anti-aging composition can promote the absorption of the above three components and increase their content in dry and sensitive skin. The synergistic effect of the four enables the skin to effectively resist external stimuli and water loss, thereby enhancing the skin barrier of dry and sensitive skin. Myricetin, a powerful antioxidant and anti-inflammatory agent, can specifically remove senescent cells, promote skin cell regeneration, and improve skin elasticity and firmness, further enhancing the anti-aging and soothing effects of the anti-aging composition. Cnidium monnieri extract, rich in osthole, has excellent anti-inflammatory and anti-allergic effects, can inhibit skin itching and allergic reactions, and alleviate discomfort caused by dry and sensitive skin. Together with the above components, it further enhances the repair, moisturizing, and soothing effects of the anti-aging composition.

[0035] For example, in different embodiments, the weight portion of the inositol can be, but is not limited to, 0.1, 0.2, 0.5, 0.7, 1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7, or 3 parts, preferably 0.5-2 parts, and more preferably 1-1.5 parts.

[0036] For example, in different embodiments, the weight portion of the ceramide-NP can be, but is not limited to, 0.01 part, 0.05 part, 0.1 part, 0.2 part, 0.5 part, 0.7 part, or 1 part, preferably 0.05-0.5 part, and more preferably 0.1-0.2 part.

[0037] For example, in different embodiments, the weight portion of the phytosphingosine can be, but is not limited to, 0.01 part, 0.05 part, 0.1 part, 0.2 part, 0.4 part, 0.6 part, 0.8 part, or 1 part, preferably 0.05-0.5 part, and more preferably 0.2-0.3 part.

[0038] For example, in different embodiments, the weight portion of the Camellia chrysantha extract can be, but is not limited to, 0.1, 0.3, 0.5, 0.7, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or 5 parts, preferably 0.5-3 parts, and more preferably 1-2 parts.

[0039] For example, in different embodiments, the weight portion of the bayberry bark extract can be, but is not limited to, 0.1, 0.2, 0.4, 0.6, 0.8, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or 5 parts, preferably 0.5-3 parts, and more preferably 1-2 parts.

[0040] For example, in different embodiments, the weight proportion of the Cnidium monnieri extract can be, but is not limited to, 0.1, 0.5, 0.8, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or 5 parts, preferably 1-4 parts, and more preferably 2-3 parts.

[0041] The present disclosure found that the weight of the components in the anti-aging composition will affect the comprehensive performance of the anti-aging composition. When the weight of each component is within the above preferred range, the obtained anti-aging composition is better in enhancing the skin barrier, alleviating sensitivity and anti-aging.

[0042] In some embodiments, in the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%, for example, but not limited to 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%.

[0043] The present disclosure study found that in the anti-aging composition, the mass percentage of Camellia chrysantha extract not only affects the penetration and absorption of the anti-aging composition, but also affects the anti-aging effect of the anti-aging composition. When the mass percentage of Camellia chrysantha extract is further selected to be within the above-mentioned range, the comprehensive effect of the obtained anti-aging composition is better, and the soothing and repairing anti-aging effect on dry and sensitive skin is better.

[0044] In some embodiments, the weight percent content of myricitrin in the bayberry bark extract is 5-15%, for example, but not limited to 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%.

[0045] In some embodiments, the mass percentage of osthole in the Cnidium monnieri extract is 2-10%, for example, but not limited to 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%.

[0046] The present disclosure found that when the mass percentage of myricitrin in the bayberry bark extract or the mass percentage of osthole in the cnidium monnieri extract is further selected within the above range, it can not only further enhance the anti-aging effect of the anti-aging composition and the soothing effect on dry skin.

[0047] In some embodiments, the method for preparing the Camellia chrysantha extract comprises the following steps:

[0048] The flowers of Camellia chrysantha are dried and crushed, and passed through an 80-150 mesh sieve to obtain Camellia chrysantha powder;

[0049] The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL.

[0050] For example, in different embodiments, the ratio of the Camellia chrysantha powder and ethanol solution can be, but is not limited to, 1g:10mL, 1g:15mL, 1g:20mL, 1g:25mL, 1g:30mL;

[0051] For example, in different embodiments, the mesh size of the Camellia chrysantha powder is 80-150 mesh; for example, it can be but not limited to 80 mesh, 100 mesh, 120 mesh, and 150 mesh.

[0052] For example, in different embodiments, the volume fraction of the ethanol solution is 60-90%, for example, but not limited to 60%, 65%, 70%, 75%, 80%, 85%, 90%.

[0053] For example, in different embodiments, the power of the ultrasonic extraction is 80-200W; for example, it can be but is not limited to 80W, 100W, 120W, 140W, 160W, 180W, and 200W.

[0054] For example, in different embodiments, the ultrasonic extraction time is 20-50 min; for example, it can be but not limited to 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min;

[0055] For example, in different embodiments, the temperature of the ultrasonic extraction is 30-40°C; for example, it can be but not limited to 30°C, 32°C, 34°C, 36°C, 38°C, or 40°C.

[0056] The present disclosure study found that the preparation method of Camellia chrysantha extract will affect the content and type of active ingredients in the Camellia chrysantha extract, and thus affect its interaction with other components. The inventors found in the actual research process that the Camellia chrysantha extract prepared using the above parameters can significantly improve the absorption of inositol, ceramide-NP and phytosphingosine, and increase the content of the three in the skin, so that the anti-aging composition has a higher repair, anti-aging and sensitivity relief effect.

[0057] In a second aspect, the invention provides the use of the anti-aging composition in the preparation of cosmetics.

[0058] In a third aspect, a cosmetic is provided, comprising the anti-aging composition, wherein the content of the anti-aging composition in the cosmetic is 1-20 wt%, for example, but not limited to 1 wt%, 3 wt%, 5 wt%, 7 wt%, 9 wt%, 11 wt%, 13 wt%, 15 wt%, 18 wt%, or 20 wt%.

[0059] In addition to the extracts mentioned above, the composition used according to the invention generally comprises a physiologically acceptable and preferably cosmetically acceptable medium, meaning a medium suitable for contact with human skin without toxicity, incompatibility, instability or allergic reactions, in particular without causing discomfort (redness, tightness, stinging). Advantageously, the cosmetic can be in the form of a powder, emulsion, microemulsion, nanoemulsion, suspension, solution, lotion, cream, aqueous or hydroalcoholic gel, foam, solution or dispersion for aerosol or lipid vesicle dispersion. In the case of an emulsion, it can be a water-in-oil or oil-in-water emulsion.

[0060] In addition to the anti-aging composition of the present disclosure, the cosmetic may further comprise:

[0061] One or more moisturizing agents, for example, selected from amino acids, chondroitin sulfate, diglycerin, erythritol, fructose, glucose, glycerin, glycerol polymers, ethylene glycol, 1,2,6-hexanetriol, honey, hyaluronic acid, hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol, maltitol, maltose, mannitol, natural moisturizing factor, PEG-15 butylene glycol, polyglyceryl sorbitol, salts of pyrrolidone carboxylic acid, potassium PCA, propylene glycol, sodium glucuronate, sodium PCA, sorbitol, sucrose, trehalose, urea, xylitol and mixtures thereof.

[0062] In certain aspects of the present invention, the cosmetics do not include an emulsifier. However, in other aspects, the cosmetics may include one or more emulsifiers. Non-limiting examples include esters of glycerol, esters of propylene glycol, fatty acid esters of polyethylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, esters of sorbitol dehydrate, carboxylic acid copolymers, esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acid amides, acyl lactylates, soaps, DEA oleyl polyoxyethylene-3 ether phosphates, and mixtures thereof.

[0063] One or more thickening agents, non-limiting examples of which include carboxylic acid polymers, cross-linked polyacrylate polymers, polyacrylamide polymers, polysaccharides, and gums. Examples of commercially available carboxylic acid polymers include carbomers, which are homopolymers of acrylic acid cross-linked with allyl ethers of sucrose or pentaerythritol. Non-limiting examples of polysaccharides include cellulose, carboxymethyl hydroxyethyl cellulose, cellulose acetate propionate carboxylate, hydroxyethyl cellulose, hydroxyethyl methyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl hydroxyethyl cellulose, microcrystalline cellulose, sodium cellulose sulfate, and mixtures thereof. Non-limiting examples of gums include gum arabic, agar, algin, alginic acid, ammonium alginate, branched starch, calcium alginate, hydrated silicon dioxide, hydroxypropyl chitosan, hydroxypropyl guar gum, karaya gum, kelp, carob bean gum, natto gum, potassium alginate, potassium carrageenan, propylene glycol alginate, sclerotium gum, sodium carboxymethyl dextran, sodium carrageenan, tragacanth gum, xanthan gum, and mixtures thereof.

[0064] Non-limiting examples of preservatives that can be used in the context of the present invention include quaternary ammonium preservatives (e.g., polyquaternium-73), parabens (e.g., methylparaben), phenoxyethanol, sorbic acid, thimerosal, 1,2-hexanediol, 1,2-pentanediol, and other polyols, or combinations thereof.

[0065] Non-limiting examples of oils that can be used in the context of the present invention include coriander oil, thyme oil, allspice oil, rose oil, impatiens oil, bergamot oil, cedar oil, chamomile oil, sage oil, clary sage oil, clove oil, fennel oil, frankincense oil, geranium oil, meadowfoam seed oil, caprylic / capric triglyceride, or combinations thereof.

[0066] In some embodiments, the cosmetic is a facial cream, and the facial cream includes the following components in percentage by weight: 1-20% anti-aging composition, 0.05-0.3% thickener, 0.5-1% moisturizer, 0.5-5% emulsifier, 0.5-3% preservative, 0.01-0.3% pH regulator, and the balance water.

[0067] For example, in different embodiments, the mass percentage of the thickener may be, but is not limited to, 0.05%, 0.1%, 0.3%, 0.5%, 1%, 1.5%, 2%, 2.5%, or 3%.

[0068] For example, in different embodiments, the mass percentage of the moisturizing agent may be, but is not limited to, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.8%, 0.9%, 0.95%, or 1%.

[0069] For example, in different embodiments, the mass percentage of the emulsifier can be, but is not limited to, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%.

[0070] For example, in different embodiments, the mass percentage of the preservative may be, but is not limited to, 0.5%, 1%, 1.5%, 2%, 2.5%, or 3%.

[0071] For example, in different embodiments, the mass percentage of the pH regulator can be, but is not limited to, 0.01%, 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, or 0.3%.

[0072] In some embodiments, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0073] In some embodiments, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0074] In some embodiments, the pH adjuster comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0075] In some embodiments, the emulsifier includes at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0076] In some embodiments, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

[0077] In order to further understand the present application, the antioxidant composition of the present application, its preparation method and its effects are further described in detail below with reference to specific examples. All raw materials involved in the present application can be obtained through commercial purchase.

[0078] The raw materials used in the examples and comparative examples are described below, but are not limited to these materials:

[0079] Camellia chrysantha extract-1 is homemade, and its preparation method includes the following steps:

[0080] Collecting Camellia chrysantha flowers, cleaning them, drying them in a 50°C constant temperature oven to constant weight, grinding them, and passing them through an 80-mesh sieve to obtain Camellia chrysantha flower powder;

[0081] Take 1g of Camellia chrysantha powder, add 20mL of 70% ethanol by volume, and perform ultrasonic extraction at 40°C for 30min with an ultrasonic extraction power of 120W. The obtained product is centrifuged and the supernatant is concentrated to 5mL to obtain Camellia chrysantha extract-1.

[0082] Camellia chrysantha extract-2 is homemade, and its preparation method includes the following steps:

[0083] Collecting Camellia chrysantha flowers, cleaning them, drying them in a 50°C constant temperature oven to constant weight, grinding them, and passing them through an 80-mesh sieve to obtain Camellia chrysantha flower powder;

[0084] Take 1g of Camellia chrysantha powder, add 30mL of 90% ethanol by volume, and perform ultrasonic extraction at 30°C for 50min with an ultrasonic extraction power of 200W. The obtained product is centrifuged and the supernatant is concentrated to 5mL to obtain Camellia chrysantha extract-2.

[0085] Camellia chrysantha extract-3 is homemade, and its preparation method includes the following steps:

[0086] Collecting Camellia chrysantha flowers, cleaning them, drying them in a 50°C constant temperature oven to constant weight, grinding them, and passing them through an 80-mesh sieve to obtain Camellia chrysantha flower powder;

[0087] Take 1 g of Camellia chrysantha powder, add 20 mL of 70% ethanol by volume, and reflux extract at 80° C. for 1 h to obtain Camellia chrysantha extract-3.

[0088] Camellia chrysantha extract-4 is homemade, and its preparation method includes the following steps:

[0089] Collecting Camellia chrysantha flowers, cleaning them, drying them in a 50°C constant temperature oven to constant weight, grinding them, and passing them through an 80-mesh sieve to obtain Camellia chrysantha flower powder;

[0090] 1 g of Camellia chrysantha flower powder was added with 20 mL of 70% ethanol by volume, and microwave extraction was performed for 30 min at a microwave power of 200 W to obtain Camellia chrysantha extract-4.

[0091] Camellia chrysantha extract-5 is homemade. Its preparation method is different from that of Camellia chrysantha extract-1 in that ethyl acetate is used instead of ethanol. The other components, weight parts, steps and preparation parameters are exactly the same.

[0092] Camellia chrysantha extract-6 is homemade. The only difference between its preparation method and the preparation method of Camellia chrysantha extract-1 is that petroleum ether is used to replace ethanol. The other components, weight parts, steps and preparation parameters are exactly the same.

[0093] Myricetin extract-1: The mass percentage of myricitrin is 10%, purchased from Xi'an Four Seasons Biotechnology Co., Ltd.

[0094] Myricetin extract-2: The mass percentage of myricitrin is 0.5%, purchased from Shaanxi Huikangyuan Biomedicine Technology Co., Ltd.

[0095] Cnidium monnieri extract-1: The mass percentage of osthole is 5%, purchased from Xi'an Shenghecui Biotechnology Co., Ltd.

[0096] Cnidium monnieri extract-2: The mass percentage of osthole is 0.5%, and it was purchased from Shaanxi Jiangu Bioengineering Co., Ltd.

[0097] Camellia extract: purchased from Shaanxi Yiruinong Biotechnology Co., Ltd.

[0098] Horse chestnut bark extract was purchased from Xi'an Meihe Biotechnology Co., Ltd.

[0099] Cassia seed extract was purchased from Shaanxi Taike Biotechnology Co., Ltd.

[0100] Inositol: purchased from Shanghai Yuanfa Biotechnology Co., Ltd.

[0101] Ceramide-NP: purchased from Guangzhou Snoco Biotechnology Co., Ltd.

[0102] Phytosphingosine: purchased from Shaanxi Peptide Source Biotechnology Co., Ltd.

[0103] Examples 1-13 and Comparative Examples 1-7

[0104] The present invention provides an anti-aging composition specifically formulated for repairing dry and sensitive skin. The components of the anti-aging composition are shown in Tables 1 and 2.

[0105] The preparation method of the anti-aging composition of Examples 1-13 and Comparative Examples 1-7 is as follows: uniformly mix the components according to the weight parts in Table 1 and Table 2 to obtain the anti-aging composition.

[0106] Table 1

[0107]

[0108]

[0109] Table 2

[0110]

[0111] Application Examples 1-16, Comparative Application Examples 1-7, and Blank Application Examples

[0112] The application examples, comparative application examples, and blank application examples of the present invention provide an anti-aging cream, the components (mass percentage) of which are shown in Table 3; wherein the anti-aging compositions used in Application Examples 1-13 are the anti-aging compositions prepared in Examples 1-13, respectively; the anti-aging compositions used in Comparative Application Examples 1-7 are the anti-aging compositions prepared in Comparative Examples 1-7, respectively; and the anti-aging compositions used in Application Examples 14-16 are the anti-aging compositions prepared in Example 1.

[0113] The preparation methods of the anti-aging creams of the application examples, comparative application examples, and blank application examples are as follows:

[0114] (1) Mixing the moisturizer, thickener, and water, heating to 85° C., and homogenizing at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component A;

[0115] (2) Mix the emulsifier, heat to 85°C, and homogenize at 1200 rpm for 4 minutes. After homogenization, keep the mixture warm for later use to obtain prefabricated component B;

[0116] (3) Mix the preservatives and heat to 60°C to melt to obtain prefabricated component C;

[0117] (4) Heat the preformed component A to 80°C, add the preformed component B at a speed of 250 rpm, stir and mix evenly, then cool to 60°C, add the preformed component C at a speed of 250 rpm, stir and mix, then cool to below 40°C, add the anti-aging composition and continue stirring for 5 minutes, finally add a pH regulator to adjust the pH to 6.0, stop stirring, discharge the material, and obtain the anti-aging cream.

[0118] Table 3

[0119]

[0120]

[0121] Effect Example 1 Penetration-enhancing and Repairing Effect Test

[0122] This effect example explores the penetration-enhancing effect and repairing effect of the anti-aging composition prepared in Examples 1-13 and Comparative Examples 1-7.

[0123] Experimental materials: The cell line used was human keratinocyte HaCaT (Beina Biotechnology).

[0124] The specific test methods are as follows:

[0125] (1) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of culture medium was added to each well. After culturing for 24 hours, the supernatant was discarded.

[0126] (2) 100 μL of DMEM medium (2105341, Gibco) was added to the blank control group, and 100 μL of DMEM medium (2105341, Gibco) and 0.01% of the anti-aging composition was added to the sample group. The cells were incubated in a 37°C, 5% CO2 incubator for 5 h and 24 h, respectively, and the cells were collected and washed three times with PBS buffer. 50 μL of RIPA cell lysis buffer containing protease inhibitors was added to each well. The lysed samples were centrifuged at 12,000 g for 5 minutes, and the supernatant was collected.

[0127] (3) Determination of tight junction protein Claudin-1 content: The Claudin-1 protein content in the cell supernatant of each group was detected using a human Claudin-1 ELISA kit (Cat. No. BLL106520E, Baililai Biotechnology).

[0128] Tight junctions are the main mode of cell connection and regulate the permeability of the skin barrier. The lower the expression level of the tight junction protein Claudin-1 after 5 hours of sample treatment, the higher the permeability of the skin barrier, and the better the penetration-promoting effect of the anti-aging composition; the higher the expression level of the tight junction protein Claudin-1 after 24 hours of sample treatment, the lower the permeability of the skin barrier, the better the effect of isolating external irritants from entering the skin, and the better the repair effect of the anti-aging composition. The penetration-promoting effect of the anti-aging composition is represented by the reduction rate of Claudin-1 in 5 hours, and the repair effect of the anti-aging composition is represented by the increase rate of Claudin-1 in 24 hours. The formula is as follows:

[0129] Claudin-1 reduction rate at 5 hours = 1 - Claudin-1 content in the sample group / Claudin-1 content in the blank group * 100%;

[0130] 24-hour Claudin-1 increase rate = (Claudin-1 content in the sample group / Claudin-1 content in the blank group - 1) * 100%;

[0131] The test results are shown in Table 4.

[0132] Table 4

[0133]

[0134] From the test results in Table 4, it can be seen that after applying the anti-aging composition of Examples 1-13 provided by the present invention, the Claudin-1 reduction rate is 30.5-54.6% in 5 hours and the Claudin-1 increase rate is 51.4-79.1% in 24 hours, indicating that the anti-aging composition of the present invention has good penetration-promoting effect and repair effect.

[0135] By comparing Example 1 with Comparative Examples 1-7, it can be seen that when any one of the camellia chrysantha extract, myrica rubra bark extract, and cnidium monnieri extract is missing or replaced with other functional analogs, the Claudin-1 reduction rate in 5 hours is less than 13%, and the Claudin-1 increase rate in 24 hours is less than 25%, indicating that the lack of any one of the camellia chrysantha extract, myrica rubra bark extract, and cnidium monnieri extract or the use of other functional analogs to replace them will lead to a decrease in the penetration-promoting effect and repair effect of the anti-aging composition.

[0136] Effect Example 2 Anti-aging Effect Test

[0137] This effect example explores the anti-aging effects of the anti-aging compositions prepared in Examples 1-13 and Comparative Examples 1-7.

[0138] Experimental materials: The cell line used was human dermal fibroblast HDF (Cat. No. PC-202h, Wuhan Saios Biotechnology Co., Ltd.), and the β-galactosidase activity assay kit was used (Cat. No. ab287846, abcam).

[0139] The specific test methods are as follows:

[0140] (1) Inoculation: Cells were inoculated into culture flasks at a density of 1×10 6 cells / mL, using DMEM high-glucose culture medium (Gibco) containing 10% fetal bovine serum, and incubated in a 37°C, 5% CO2 incubator for 24 h;

[0141] (2) Induction: Treat with PBS buffer containing 50 μM hydrogen peroxide for 30 minutes, discard the PBS containing hydrogen peroxide, and then wash with PBS three times;

[0142] (3) Sampling: The blank control group was added with DMEM high-glucose culture medium containing 10% fetal bovine serum, and the sample group was added with DMEM high-glucose culture medium containing 10% fetal bovine serum and 0.01% anti-aging composition. The cells were cultured in a 37°C, 5% CO2 incubator for 24 hours, and the supernatant was collected.

[0143] (4) β-galactosidase activity assay: A β-galactosidase activity assay kit (Cat. No. ab287846, abcam) was used to detect the senescence level of cells.

[0144] Hydrogen peroxide induces cellular senescence, which is marked by an increase in β-galactosidase activity. Therefore, after sample treatment, the lower the β-galactosidase activity, the lower the level of cellular senescence and the stronger the anti-aging ability of the repair and anti-aging composition. The anti-aging ability of the repair and anti-aging composition is represented by the decrease in β-galactosidase activity, and the calculation formula is as follows:

[0145] Cell senescence improvement rate = 1-β-galactosidase activity 样品组 / β-galactosidase activity 对照组 *100%;

[0146] The test results are shown in Table 5.

[0147] Table 5

[0148]

[0149]

[0150] From the test results in Table 5, it can be seen that after applying the anti-aging compositions of Examples 1-13 provided by the present invention, the cell aging improvement rate is 54.1-81.9%, indicating that the anti-aging compositions of the present invention have a good anti-aging effect.

[0151] By comparing Example 1 with Comparative Examples 1-7, it can be seen that the cell aging improvement rate is lower than 26% when any one of the camellia chrysantha extract, myrica rubra bark extract, and cnidium monnieri extract is missing or replaced with other functional analogs, indicating that the anti-aging effect of the anti-aging composition is reduced when any one of the camellia chrysantha extract, myrica rubra bark extract, and cnidium monnieri extract is missing or replaced with other functional analogs.

[0152] Effect Example 3 Human Skin Patch Test

[0153] This effect example explores the irritation of the anti-aging cream prepared in Application Examples 1-16 and Comparative Application Examples 1-7 to the skin.

[0154] Thirty volunteers (15 men and 15 women, aged 20-50 years) were recruited. A closed patch test was performed. An equal amount (0.020 mL to 0.025 mL) of the test sample was placed in a specific patch tester. The patch was then applied to the volunteer's arm with hypoallergenic tape, gently pressing to evenly adhere to the skin. The patch was left on for 24 hours. A blank control group received distilled water, and a negative control group received a blank anti-aging cream. After 24 hours, the patch tester was removed, and skin reactions were observed 0.5, 24, and 48 hours later, with the results recorded. The severity of adverse skin reactions is shown in Table 6 below.

[0155] Table 6

[0156]

[0157] The anti-aging creams provided in Application Examples 1-16 of the present invention and Comparative Application Examples 1-7 all showed negative reactions after human patch tests, indicating that they are safe and non-irritating to human skin, indicating that the anti-aging composition of the present invention has low irritation.

[0158] Effect Example 4 Human Efficacy Test

[0159] This effect example tests the improvement effects of Application Examples 1-16, Comparative Application Examples 1-7 and Blank Application Examples on lipidomics, repair, moisturizing, wrinkle reduction and soothing of dry and sensitive skin.

[0160] The specific test methods are as follows:

[0161] Through lipidomics research on sensitive skin, the inventors found that dry sensitive skin, compared with healthy skin and oily sensitive skin, lacks three lipid components: hemolytic phosphatidylinositol, ceramide-NP and sphingosine, which leads to damage to the skin lipid barrier, dryness, redness and susceptibility to fine lines. Therefore, after volunteers use the repair and anti-aging composition, the higher the content of these three lipid components, the better the effect of the composition in repairing the skin lipid barrier.

[0162] According to the "Technical Specifications for Safety of Cosmetics" (2015), 120 Asian adults aged 45-60 with dry, sensitive skin and wrinkles around the eyes were randomly divided into 24 groups of 5 people each. Under normal circumstances, volunteers continued to use the product on their entire face for 28 days (T28). Every morning and evening, after cleansing and moisturizing, volunteers applied an appropriate amount of anti-aging cream to their face and massaged it in for absorption. This was done once a day and once a night. Data were collected before and 28 days after use. On the day of the visit, volunteers washed their faces with clean water without applying any product. They then sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 2 hours. Sebum was collected from the forehead and cheeks of volunteers five times using skin tape D-SquameTM D100. Quantitative lipidome sequencing was performed using a quantitative lipidome sequencer from Wuhan Maiwei Metabolic Biotechnology Co., Ltd. The average levels of lysophosphatidylinositol, ceramide-NP, and sphingosine in the skin of the volunteers were calculated based on the sequencing results, and the corresponding content increase rates were calculated as follows: lipid increase rate = (T28 样品组 -T0 样品组 ) / T0 样品组 *100%]-[(T28 空白应用例 -T0 空白应用例 ) / T0 空白应用例 *100%].

[0163] At the same time, the transepidermal water loss (TEWL) of the zygomatic bone was measured using the TewaMeter skin moisture loss test probe, the skin moisture content of the zygomatic bone was measured using the Corneometer skin moisture test probe, facial wrinkles were photographed and analyzed using VISIA-CR, and the skin discomfort of the volunteers was assessed using a 10-point self-assessment questionnaire (the higher the score, the stronger the discomfort).

[0164] The skin barrier repair ability is represented by the improvement in TEWL value, the moisturizing effect is represented by the improvement in skin moisture content, the wrinkle reduction effect is represented by the improvement in the area of ​​wrinkles at the corners of the eyes, and the soothing effect is represented by the improvement in the self-assessment score of skin discomfort. The formula is as follows:

[0165] TEWL value improvement rate = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用

[0166] 例 ) / T0 空白应用例 *100%];

[0167] Skin moisture content improvement rate = (T28 样品组 -T0样品组 ) / T0 样品组 *100%]-[(T28 空白应用例 -T0 空白应用例 ) / T0 空白应用例 *100%];

[0168] Improvement rate of eye wrinkles=(T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用例 ) / T0 空白应用例 *100%];

[0169] Skin discomfort improvement rate = (T0 样品组 -T28 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -T28 空白应用例 ) / T0 空白应用例 *100%].

[0170] The results are shown in Table 7.

[0171] Table 7

[0172]

[0173]

[0174] From the experimental results in Table 7, it can be seen that when the anti-aging creams of Application Examples 1-16 of the present invention are used, the increase rate of lysophosphatidylinositol in dry and sensitive skin is 35.8-89.3%, the increase rate of ceramide-NP is 17.2-37.7%, the increase rate of sphingosine is 23.4-44.9%, the TEWL value improvement rate is 25.3-57.2%, the skin moisture content improvement rate is 21.6-41.3%, the improvement rate of wrinkles at the corners of the eyes is 12.6-23%, and the improvement rate of skin discomfort is 54.8-85.5%; this shows that the application of the anti-aging composition of the present invention can effectively increase lysophosphatidylinositol, ceramide-NP and sphingosine in dry and sensitive skin, improve the skin's TEWL, moisture content, wrinkles at the corners of the eyes and discomfort, that is, the anti-aging composition of the present invention has good repairing, moisturizing, anti-aging and soothing effects.

[0175] It can be seen from Application Example 1 and Comparative Application Examples 1-7 that the lack of any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract or the use of other functional analogs for replacement, the increase rate of hemolytic phosphatidyl inositol in dry and sensitive skin is less than 32%, the increase rate of ceramide-NP is less than 10%, the increase rate of sphingosine is less than 9.5%, the TEWL value improvement rate is less than 8%, the skin moisture content improvement rate is less than 9%, the improvement rate of wrinkles at the tail of the eyes is less than 6%, and the skin discomfort improvement rate is less than 14.5%, indicating that the lack of any one of Camellia chrysantha extract, Bayberry bark extract, and Cnidium monnieri extract or the use of other functional analogs for replacement will lead to a decrease in the anti-aging effect of the anti-aging composition.

[0176] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-aging composition, characterized in that The anti-aging composition comprises the following components in parts by weight: 0.1-3 parts of inositol, 0.01-1 parts of ceramide-NP, 0.01-1 parts of phytosphingosine, 0.1-5 parts of golden camellia extract, 0.1-5 parts of myrica rubra bark extract and 0.1-5 parts of cnidium monnieri extract; The preparation method of the Camellia chrysantha extract comprises the following steps: The flowers of Camellia chrysantha are dried and crushed, and passed through an 80-150 mesh sieve to obtain Camellia chrysantha powder; The camellia chrysantha powder is added into an ethanol solution for extraction and concentrated into a paste to obtain the camellia chrysantha extract; the solid-liquid ratio of the camellia chrysantha powder to the ethanol solution is 1g:10-30mL; the extraction is ultrasonic extraction or microwave extraction; and the volume fraction of the ethanol solution is 60-90%.

2. The anti-aging composition according to claim 1, wherein The anti-aging composition comprises the following components in parts by weight: 0.5-2 parts of inositol, 0.05-0.5 parts of ceramide-NP, 0.05-0.5 parts of phytosphingosine, 0.5-3 parts of golden camellia extract, 0.5-3 parts of myrica rubra bark extract and 1-4 parts of cnidium monnieri extract.

3. The anti-aging composition according to claim 2, wherein The anti-aging composition comprises the following components in parts by weight: 1-1.5 parts of inositol, 0.1-0.2 parts of ceramide-NP, 0.2-0.3 parts of phytosphingosine, 1-2 parts of golden camellia extract, 1-2 parts of bayberry bark extract and 2-3 parts of cnidium monnieri extract.

4. The anti-aging composition according to claim 1, wherein In the anti-aging composition, the mass percentage of the Camellia chrysantha extract is 14-30%; and / or the mass percentage of myricitrin in the Myrica rubra bark extract is 5-15%; and / or the mass percentage of osthole in the Cnidium monnieri extract is 2-10%.

5. The anti-aging composition according to claim 1, wherein The temperature of the ultrasonic extraction is 30-40° C., the time of the ultrasonic extraction is 20-50 min, and the power of the ultrasonic extraction is 80-200W.

6. Use of the anti-aging composition according to any one of claims 1 to 5 in the preparation of cosmetics.

7. A cosmetic, characterized in that: The cosmetic comprises the anti-aging composition according to any one of claims 1 to 5, wherein the content of the anti-aging composition in the cosmetic is 1 to 20 wt %.

8. The cosmetic according to claim 7, wherein The cosmetic is a facial cream, and the facial cream comprises the following components in percentage by mass: 1-20% of an anti-aging composition, 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 0.5-5% of an emulsifier, 0.5-3% of a preservative, 0.01-0.3% of a pH regulator, and the balance of water.

9. The cosmetic according to claim 8, wherein The thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; And / or, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerin, budding pullulan polysaccharide, and ceramide; and / or, the pH regulator comprises at least one of arginine, tromethamine, and EDTA-disodium; and / or, the emulsifier comprises at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraisostearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; And / or, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone.

Images