Sample processing and scheduling system and control method
By optimizing the rotation cycle of the reaction disk assembly and the movement of the needle assembly, the problems of slow sample processing speed and high cost were solved, achieving efficient and low-cost sample processing.
Patent Information
- Application Number
- CN202411593838.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-08
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2044-11-08
AI Technical Summary
Existing sample processing methods are slow and costly, especially in sample dilution and retesting operations, which leads to reduced testing speed and shorter consumable lifespan.
By controlling the rotation cycle and rotation phase of the reaction disk assembly, combined with the actions of the sampling needle and reagent needle assemblies, the sample processing flow is optimized, reducing pretreatment and retesting dilution operations, thus achieving efficient sample processing.
It improved sample processing speed, reduced costs, decreased the amount of samples and consumables used, and extended component lifespan.
Smart Images
Figure CN119438609B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of sample processing, in particular to a sample processing and scheduling system and a control method. BACKGROUND
[0002] In the sample testing of a medical equipment in a clinical laboratory, a general need exists for pretreatment operations such as dilution or hemolysis of samples and retesting operations.
[0003] In the pretreatment operation of sample dilution in the prior art, a manufacturer usually uses the next testing period to perform a pretreatment operation, i.e., a dilution or hemolysis pretreatment is performed using a testing period originally planned for a new sample. This control method reduces the testing speed of the entire machine, and each time a pretreatment operation is added, the testing speed is reduced by 50%. In addition, in the retesting operation of sample dilution, the manufacturer usually performs a second dilution operation by again taking a sample, which is equivalent to performing two tests. Not only does this increase the use of samples and the demand for individual samples, but it also increases the use of components such as sampling needles and reduces the service life of various consumables.
[0004] In summary, the existing sample processing speed is low and the cost is high. SUMMARY
[0005] Therefore, the embodiments of the present application provide a sample processing and scheduling system and a control method, which can effectively solve the problems of low sample processing speed and high cost.
[0006] In a first aspect, the embodiments of the present application provide a control method of a sample processing and scheduling system, the sample processing and scheduling system comprising a reaction disc assembly, a sampling needle assembly, and a reagent needle assembly; the method comprising:
[0007] controlling rotation of the reaction disc assembly according to a rotation period, and each rotation period controlling each cup in the reaction disc assembly to progress by a preset cup position number; wherein the rotation period comprises a plurality of rotation stages; the length of the rotation period and the interval length of the plurality of rotation stages are determined according to a target reaction system;
[0008] determining a target dilution cup and a target reaction cup in the reaction disc assembly according to a first target rotation period, the preset cup position number, and a total number of cup positions in the reaction disc assembly;
[0009] controlling the sampling needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup according to target rotation periods of corresponding actions of sample processing tasks, such as sample taking and adding, reagent adding, diluent adding, and dilution sample adding, to complete the sample processing tasks.
[0010] In some embodiments, the control of the sampling needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup comprises:
[0011] According to the corresponding execution positions of the sampling needle assembly and the reagent needle assembly, the sampling needle assembly and the reagent needle assembly are controlled to perform corresponding actions on the target dilution cup and the target reaction cup, respectively; wherein the execution positions of the sampling needle assembly and the reagent needle assembly are determined according to each target rotation period, the preset number of cup positions, the total number of cup positions, and the number of cup positions rotated in each rotation stage, respectively; each target rotation period is determined according to the target reaction system;
[0012] The sample processing and scheduling system further comprises a wiping assembly; the wiping position of the wiping assembly is fixedly arranged at the preset cup position of the reaction disc assembly.
[0013] In some embodiments, each rotation period comprises three rotation stages; the control of the rotation of the reaction disc assembly according to the rotation period comprises:
[0014] In each rotation period, the rotation of the reaction disc assembly is controlled by C1 cup positions in the first rotation stage, C2 cup positions in the second rotation stage, and C3 cup positions in the third rotation stage.
[0015] And / or,
[0016] According to the target rotation period corresponding to the actions of sample suction and sample, reagent, diluent, and diluted sample in the sample processing task, the sampling needle assembly and the reagent needle assembly are controlled to perform corresponding actions on the target dilution cup and the target reaction cup, respectively, comprising:
[0017] In the nth rotation period, the diluent needle is controlled to add diluent to the target dilution cup, and the wiping assembly is controlled to wipe the target reaction cup at the preset cup position;
[0018] In the n2th rotation period, the sampling needle assembly is controlled to add the original sample to the target dilution cup at the sample adding position;
[0019] In the n3th rotation period, the reagent needle assembly is controlled to add reagent to the target reaction cup at the reagent adding position;
[0020] after determining retesting, after the end of the first rotation stage of the nth4 rotation cycle, controlling the sampling needle assembly to suck the retest-use dilution sample from the target dilution cup at the retest sample suction position; after the end of the second rotation stage of the nth4 rotation cycle, adding the sucked retest-use dilution sample to the target reaction cup; wherein the retest sample suction position is calculated according to the nth1 rotation cycle, the nth4 rotation cycle, the preset cup position number, the number of cups rotated in the first rotation stage, and the total number of cup positions.
[0021] In some embodiments, the target rotation cycle corresponding to the actions of sucking and adding samples, reagents, diluents, and diluted samples in the sample processing task is used to control the sampling needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup, respectively, including:
[0022] after the end of the third rotation stage of the nth1 rotation cycle, controlling the diluent needle to add diluent to the target dilution cup, and controlling the wiping assembly to wipe the target reaction cup at the preset cup position;
[0023] after the end of the second rotation stage of the nth2 rotation cycle, controlling the sampling needle assembly to add the original sample to the target dilution cup at the sample addition position; wherein the sample addition position is calculated according to the nth1 rotation cycle, the nth2 rotation cycle, the preset cup position number, the number of cups rotated in the third rotation stage, and the total number of cup positions;
[0024] after the end of the first rotation stage of the nth3 rotation cycle, controlling the reagent needle assembly to add reagent to the target reaction cup at the reagent addition position; wherein the reagent addition position is calculated according to the nth1 rotation cycle, the nth3 rotation cycle, the preset cup position number, the number of cups rotated in the second rotation stage, the number of cups rotated in the third rotation stage, and the total number of cup positions;
[0025] after determining retesting, after the end of the first rotation stage of the nth4 rotation cycle, controlling the sampling needle assembly to suck the retest-use dilution sample from the target dilution cup at the retest sample suction position; after the end of the second rotation stage of the nth4 rotation cycle, adding the sucked retest-use dilution sample to the target reaction cup; wherein the retest sample suction position is calculated according to the nth1 rotation cycle, the nth4 rotation cycle, the preset cup position number, the number of cups rotated in the first rotation stage, and the total number of cup positions.
[0026] In some embodiments, the sample processing and scheduling system further includes a cleaning assembly; the method further includes:
[0027] After the third rotation stage of the n5th rotation cycle ends, the cleaning assembly is controlled to clean the target dilution cup at a cleaning position, wherein the cleaning position is calculated according to the n1th rotation cycle, the n5th rotation cycle, the preset cup number and the total cup number.
[0028] After the second rotation stage of the n5th rotation cycle ends, the cleaning assembly is controlled to clean the target reaction cup at the cleaning position.
[0029] In some embodiments, the cleaning position is calculated by the following formula:
[0030] W = ((n5 + 1 - n1) x g + 1) % C
[0031] wherein W is the cup number corresponding to the cleaning position in the reaction disc assembly, n5 is the serial number of the n5th rotation cycle, n1 is the serial number of the n1th rotation cycle, g is the preset cup number, and C is the total cup number in the reaction disc assembly.
[0032] In some embodiments, the preset cup number is calculated by the following formula:
[0033] g = | (C1 + C2 + C3) % C |
[0034] wherein C1 is the number of cups rotated in the first rotation stage, C2 is the number of cups rotated in the second rotation stage, C3 is the number of cups rotated in the third rotation stage, C is the total cup number in the reaction disc assembly, and g is the preset cup number.
[0035] The sample adding position is calculated by the following formula:
[0036] S = ((n2 - n1) x g + C3 + 1) % C
[0037] wherein S is the cup number corresponding to the sample adding position in the reaction disc assembly, n1 is the serial number of the n1th rotation cycle, and n2 is the serial number of the n2th rotation cycle.
[0038] The reagent adding position is calculated by the following formula:
[0039] R = ((n3 - n1) x g + C2 + C3) % C
[0040] wherein R is the cup number corresponding to the reagent adding position in the reaction disc assembly, and n3 is the serial number of the n3th rotation cycle.
[0041] The sample weighing and measuring position is calculated by the following formula:
[0042] S1 = ((n4 + 1 - n1) x g - C1 + 1) % C
[0043] Wherein, S1 is the sample site corresponding to the cup number in the reaction disc assembly; n4 is the sequence number of the n4th rotation cycle.
[0044] In some embodiments, the method further comprises:
[0045] In some embodiments, the method further comprises:
[0046] In some embodiments, the method further comprises:
[0047] In some embodiments, the method further comprises:
[0048] In some embodiments, the method further comprises:
[0049] In some embodiments, the method further comprises:
[0050] x1=(n1×g)%C
[0051] x2=x1-1
[0052] Wherein, x1 is the cup number of the target dilution cup; x2 is the cup number of the target reaction cup; n1 is the sequence number of the first target rotation cycle; C is the total number of cups in the reaction disc assembly; g is the preset cup number.
[0053] In some embodiments, the method further comprises:
[0054] Embodiments of the present application have the following beneficial effects:
[0055] In the application, the reaction disc assembly is controlled to rotate according to a rotation period, and each rotation period controls the cups in the reaction disc assembly to advance by a preset cup position number; wherein, the rotation period includes multiple rotation stages; the length of the rotation period and the interval length of the multiple rotation stages are determined according to a target reaction system; according to a first target rotation period, the preset cup position number, and the total number of cup positions in the reaction disc assembly, a target dilution cup and a target reaction cup are determined in the reaction disc assembly; according to the target rotation period of the corresponding actions of sample suction and sample, reagent, diluent, and diluted sample in a sample processing task, the sampling needle assembly and the reagent needle assembly are controlled to perform corresponding actions on the target dilution cup and the target reaction cup, so as to complete the sample processing task. In the application, the reaction cup and the dilution cup are included, without additional pre-treatment, and when retesting, the diluted sample is directly sucked from the dilution cup, without re-dilution operation, so that the sample processing speed is high, and the cost is low. BRIEF DESCRIPTION OF DRAWINGS
[0056] In order to more clearly illustrate the technical solutions of the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some of the embodiments of the application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.
[0057] Figure 1 A structural schematic diagram of a sample processing and scheduling system according to an embodiment of the application is shown;
[0058] Figure 2 A flowchart of a control method of a sample processing and scheduling system according to an embodiment of the application is shown;
[0059] Figure 3 A timing diagram of a control method of a sample processing and scheduling system according to an embodiment of the application is shown;
[0060] Figure 4 Another timing diagram of a control method of a sample processing and scheduling system according to an embodiment of the application is shown;
[0061] Figure 5 An execution position schematic diagram of various components in a control method of a sample processing and scheduling system according to an embodiment of the application is shown;
[0062] Figure 6 A structural schematic diagram of a control device of a sample processing and scheduling system according to an embodiment of the application is shown.
[0063] Main element symbol explanation:
[0064] 110 - reaction disc assembly; 120 - sampling needle assembly; 130 - reagent needle assembly; 140 - wiping assembly; 150 - cleaning assembly; 610 - reaction disc control module; 620 - dilution cup reaction cup determination module; 630 - reagent sample operation module. DETAILED DESCRIPTION
[0065] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all of the embodiments of the present application.
[0066] The components of the embodiments of the present application generally described and illustrated herein can be arranged and designed in a wide variety of different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the application, but merely represents selected embodiments of the application. All other embodiments obtained from the embodiments of the present application by those skilled in the art without creative work are within the scope of the present application.
[0067] Hereinafter, the terms "include", "have", and their conjugates used in the various embodiments of the present application are only intended to denote a certain characteristic, number, step, operation, element, component, or combination of the foregoing, and should not be construed as excluding the presence or addition of one or more other characteristics, numbers, steps, operations, elements, components, or combinations thereof. In addition, the terms "first", "second", "third", and the like are used only to distinguish the description, and cannot be understood as indicating or implying relative importance.
[0068] Unless otherwise defined, all terms used herein (including technical terms and scientific terms) have the same meaning as commonly understood by those skilled in the art to which the various embodiments of the present application belong. The terms (such as those defined in a generally used dictionary) will be interpreted as having the same meaning as the contextual meaning in the relevant technical field and will not be interpreted as having an idealized or overly formal meaning, unless clearly defined in the various embodiments of the present application.
[0069] Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments and features in the embodiments can be combined with each other without conflict.
[0070] The prior art is used to dilute or hemolyze the test cycle originally planned for new samples, but each additional pretreatment reduces the speed by 50%. In addition, when the sample is diluted and retested, a second dilution operation is required, which increases the use of samples, increases the hospital's blood sampling requirements for individuals, and also increases the use of components such as sampling needles and reduces the service life of various consumables. Therefore, the present application provides a sample processing and scheduling system and a control method to improve sample processing speed and reduce costs.
[0071] As shown in Figure 1 The present application provides a sample processing and scheduling system, which exemplarily comprises a controller, a reaction disc assembly 110, a sampling needle assembly 120, and a reagent needle assembly 130. Further, the system also comprises a wiping assembly 140 and a cleaning assembly 150. The controller implements sample processing tasks using a sample processing and scheduling system control method provided by the present application.
[0072] The sample processing and scheduling system control method will be described below in conjunction with some specific embodiments.
[0073] Figure 2 A flowchart of the sample processing and scheduling system control method of the present application is shown. The sample processing and scheduling system control method exemplarily comprises the following steps:
[0074] S100, according to the rotation period, the reaction disc assembly 110 is controlled to rotate, and each rotation period controls the cups in the reaction disc assembly 110 to advance by a preset number of cup positions; wherein the rotation period comprises a plurality of rotation stages; the length of the rotation period and the interval length of the plurality of rotation stages are determined according to the target reaction system. Each rotation period controls the cups in the reaction disc assembly 110 to advance or retreat by a preset number of cup positions.
[0075] Exemplarily, each rotation period comprises 3 rotation stages; the reaction disc assembly 110 is controlled to rotate according to the rotation period, which comprises:
[0076] In each rotation period, the reaction disc assembly 110 is controlled to rotate by C1 cup positions in the first rotation stage, by C2 cup positions in the second rotation stage, and by C3 cup positions in the third rotation stage.
[0077] Further, the preset number of cup positions is calculated using the following formula:
[0078] g = | (C1 + C2 + C3) % C |
[0079] C1+C2+C3) % C = 2, if the reaction disc rotates clockwise. If the reaction disc rotates anticlockwise, the preset cup number is negatively progressed, (C1+C2+C3) % C = -2, and C1, C2, C3 are all negative values when the reaction disc rotates anticlockwise.
[0080] The reaction disc assembly 110 progresses 2 cups per rotation cycle. That is, the preset cup number includes two cups, one dilution cup and one reaction cup. If the reaction disc rotates clockwise, the preset cup number is positively progressed, (C1+C2+C3) % C = 2. If the reaction disc rotates anticlockwise, the preset cup number is negatively progressed, (C1+C2+C3) % C = -2, and C1, C2, C3 are all negative values when the reaction disc rotates anticlockwise.
[0081] S200, determining a target dilution cup and a target reaction cup in the reaction disc assembly 110 according to the first target rotation cycle, the preset cup number, and the total number of cups in the reaction disc assembly 110.
[0082] S300, controlling the sampling needle assembly 120 and the reagent needle assembly 130 to perform corresponding actions on the target dilution cup and the target reaction cup according to the target rotation cycles corresponding to the actions of aspirating and dispensing samples, reagents, diluents, and diluted samples in the sample processing task, so as to complete the sample processing task.
[0083] Further, in step S300, the control of the sampling needle assembly 120 and the reagent needle assembly 130 to perform corresponding actions on the target dilution cup and the target reaction cup comprises:
[0084] According to the execution positions of the sampling needle assembly 120 and the reagent needle assembly 130, the sampling needle assembly 120 and the reagent needle assembly 130 are controlled to perform corresponding actions on the target dilution cup and the target reaction cup, respectively; wherein the execution positions of the sampling needle assembly 120 and the reagent needle assembly 130 are determined according to each target rotation cycle, the preset cup number, the total number of cups, and the number of cups rotated in each rotation stage, respectively; each target rotation cycle is determined according to the target reaction system. The sample processing and scheduling system further comprises a wiping assembly 140; the wiping position of the wiping assembly 140 is fixedly arranged at a preset cup position of the reaction disc assembly 110. Illustratively, the wiping position of the wiping assembly 140 is fixedly arranged at the 0th cup position. The total number of cups of the reaction disc assembly 110 is C, that is, the total number of cups in the reaction disc assembly 110 is C, and one cup is placed in one cup position. The sample loading position is S, S1 is the sample weight measurement position, the reagent loading position is R, and the cleaning position is W.
[0085] In one rotation cycle, the reaction disc assembly 110 rotates C1 cups for the first time,Figure 3 10a; the second time C2 cups are turned, Figure 3 10b; the third time C3 cups are turned, Figure 3 10c. The wiping position is set as the 0th cup, and S, R and W are all positions relative to the wiping position. The position of the sample needle assembly 120 performing the injecting original sample action is the sample adding position S, the position of the sample needle assembly 120 performing the sucking re-measuring action is the re-measuring sample sucking position S1, the position of the reagent needle assembly 130 performing the injecting reagent action is the reagent adding position R, and the position of the washing assembly 150 performing the washing action is the washing position W.
[0086] According to the different reaction times in the reaction system, the application sets the target rotation periods corresponding to the sucking and injecting samples, reagents, diluents and diluted samples, i.e., sets the subsequent target rotation periods n1, n2, n3, n4 and n5. Understandably, the method further comprises:
[0087] Before controlling the rotation of the reaction disc assembly 110, the reaction disc assembly 110 is also initialized and reset, specifically including setting the cup numbers of the cups in the reaction disc assembly 110 to be equal to the cup position numbers of the reaction disc assembly 110; and controlling the wiping assembly 140 in the sample processing and scheduling system to be at the 0th cup position. Demonstratively, after the instrument is started, when the reset is completed, the 0th cup position corresponds to the 0th cup, and the 1st cup position corresponds to the 1st cup, i.e., the cup positions are arranged in counterclockwise order from the 0th cup position. After the reset, it further includes: controlling the wiping assembly to wipe the 0th cup at the 0th cup position, and controlling the diluent needle to add diluent to the 1st cup at the 1st cup position. Wherein, the wiping assembly is fixed at the 0th cup position.
[0088] According to the first target rotation period, the preset cup number and the total number of cup positions in the reaction disc assembly 110, the target dilution cup and the target reaction cup are determined in the reaction disc assembly 110, including:
[0089] The cup numbers of the target dilution cup and the target reaction cup are calculated according to the following calculation formula:
[0090] x1 = (n1 x g) % C
[0091] x2 = x1 - 1
[0092] Wherein, x1 is the cup number of the target dilution cup; x2 is the cup number of the target reaction cup; n1 is the serial number of the first target rotation period; C is the total number of cup positions contained in the reaction disc assembly 110, and g is the preset cup number. Preferably, g = 2, which includes one reaction cup and one dilution cup. That is, x1 = (n1 x 2) % C, x2 = x1 - 1, and the reaction cup and the dilution cup are adjacent.
[0093] Further, each of the rotation periods comprises three rotation stages; and the rotating the reaction disc assembly according to the rotation periods comprises:
[0094] controlling the reaction disc assembly to rotate C1 cup positions in a first rotation stage, C2 cup positions in a second rotation stage, and C3 cup positions in a third rotation stage in each rotation period.
[0095] controlling the sampling needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup according to target rotation periods corresponding to actions of drawing and adding samples, reagents, diluents, and diluted samples in the sample processing task, comprises:
[0096] in the nth1 rotation period, controlling the diluent needle to add diluents to the target dilution cup, and controlling the wiping assembly to wipe the target reaction cup at the preset cup position.
[0097] in the nth2 rotation period, controlling the sampling needle assembly to add the original sample to the target dilution cup at the sample adding position.
[0098] in the nth3 rotation period, controlling the reagent needle assembly to add the reagent to the target reaction cup at the reagent adding position.
[0099] after determining to retest, in the nth4 rotation period, controlling the sampling needle assembly to draw the diluted sample from the target dilution cup at the retest sample drawing position; wherein n4>n3>n2>nl.
[0100] Further, before the reaction disc assembly is controlled to rotate, the reaction disc assembly is also controlled to initialize and reset. If after the reset, cup No. 2 is at cup position No. 0 and cup No. 3 is at cup position No. 1, then after the third rotation stage of the 0th rotation period ends, cup No. 0 is at cup position No. 0 and cup No. 1 is at cup position No. 1, as Figure 3 corresponding to the operation of 13a in the middle, cup No. 0 is wiped and cup No. 1 is added with diluents. If after the reset, cup No. 0 is at cup position No. 0 and cup No. 1 is at cup position No. 1, then before the 0th rotation period rotates, cup No. 0 is immediately at cup position No. 0 and cup No. 1 is at cup position No. 1, cup No. 0 is wiped and cup No. 1 is added with diluents.
[0101] Further, in step S300, controlling the sampling needle assembly 120 and the reagent needle assembly 130 to perform corresponding actions on the target dilution cup and the target reaction cup according to target rotation periods corresponding to actions of drawing and adding samples, reagents, diluents, and diluted samples in the sample processing task, comprises:
[0102] S310, after the end of the third rotation stage of the nth1 rotation period, control the diluent needle to add diluent into the target dilution cup; control the wiping assembly 140 to wipe the target reaction cup. In a counterclockwise direction, the diluent needle is in the next cup position of the wiping position. Illustratively, after resetting, cup No. 2 is in cup position No. 0 and cup No. 3 is in cup position No. 1, then after the end of the third rotation stage of the 0th rotation period, cup No. 0 is in cup position No. 0 and cup No. 1 is in cup position No. 1, as shown in FIG. 6B. Figure 3 the operation corresponding to 13a in FIG. 11B.
[0103] S320, after the end of the second rotation stage of the nth2 rotation period, control the sampling needle assembly 120 to add the original sample to the target dilution cup at the sample adding position, as shown in Figure 3 the operation corresponding to 11b in FIG. 11B; wherein the sample adding position is calculated according to the nth1 rotation period, the nth2 rotation period, the preset number of cup positions, the number of cups rotated in the third rotation stage, and the total number of cup positions.
[0104] Illustratively, the sample adding position is calculated using the following formula:
[0105] S = ((n2-n1) x g + C3 + 1) % C
[0106] wherein S is the cup position number corresponding to the sample adding position in the reaction disc assembly 110, n1 is the serial number of the nth1 rotation period, and n2 is the serial number of the nth2 rotation period.
[0107] S330, after the end of the first rotation stage of the nth3 rotation period, control the reagent needle assembly 130 to add reagent to the target reaction cup at the reagent adding position, as shown in Figure 3 the operation corresponding to 12a in FIG. 12B; wherein the reagent adding position is calculated according to the nth1 rotation period, the nth3 rotation period, the preset number of cup positions, the number of cups rotated in the second rotation stage, the number of cups rotated in the third rotation stage, and the total number of cup positions. Illustratively, the reagent adding position is calculated using the following formula:
[0108] R = ((n3-n1) x g + C2 + C3) % C
[0109] wherein R is the cup position number corresponding to the reagent adding position in the reaction disc assembly 110, and n3 is the serial number of the nth3 rotation period.
[0110] S340, after determining to retest, after the end of the first rotation stage of the nth4 rotation period, control the sampling needle assembly to suck the dilution sample for retesting from the target dilution cup at the weight measurement and sampling position, as shown in Figure 3corresponding to the 11a in the middle; after the end of the second rotation stage of the n4th rotation period, the diluted sample sucked by the re-measurement sample site is added to the target reaction cup; wherein, the re-measurement sample site is calculated according to the n1th rotation period, the n4th rotation period, the preset number of cup sites, the number of cups rotated in the first rotation stage, and the total number of cup sites. Wherein, the re-measurement sample site is calculated according to the n1th rotation period, the n4th rotation period, the preset number of cup sites, the number of cups rotated in the first rotation stage, and the total number of cup sites; wherein, n4>n3>n2>n1. The re-measurement sample site is calculated using the following formula:
[0111] S1=((n4+1-n1)×g-C1+1)%C
[0112] Wherein, S1 is the cup site number corresponding to the re-measurement sample site in the reaction disc assembly 110, and n4 is the serial number of the n4th rotation period.
[0113] The method further comprises:
[0114] S350, after the end of the third rotation stage of the next rotation period of the n3th rotation period, controlling the sampling needle assembly 120 to suck diluted sample from the target diluted cup at the re-measurement sample site, and then controlling the sampling needle to discharge the diluted sample into the target reaction cup at the sample adding site.
[0115] Further, the sample processing and scheduling system further comprises a cleaning assembly 150. The method further comprises:
[0116] S360, after the end of the third rotation stage of the n5th rotation period, controlling the cleaning assembly 150 to clean the target diluted cup at the cleaning site, such as Figure 3 corresponding to the 14b in the middle; wherein, the cleaning site is calculated according to the n1th rotation period, the n5th rotation period, the preset number of cup sites, and the total number of cup sites. After the end of the second rotation stage of the n5th rotation period, control the cleaning assembly 150 to clean the target reaction cup according to the cleaning site, such as Figure 3 corresponding to the 14a in the middle.
[0117] Further, the cleaning site is calculated using the following formula:
[0118] W=((n5+1-n1)×g+1)%C
[0119] Wherein, W is the corresponding cup position number of the cleaning position in the reaction disc assembly 110, n5 is the serial number of the n5th rotation cycle, n1 is the serial number of the n1th rotation cycle, g is the preset cup position number, and C is the total number of cup positions in the reaction disc assembly 110.
[0120] The control method of the sample processing and scheduling system of the present application will be described below in combination with a specific example. In the present example, C = 60, C1 = -22, C2 = -41, C3 = -59, and each rotation cycle is negatively progressive by (C1 + C2 + C3) % C cups, i.e. 2 cups. After the reaction disc is reset, the 0th cup position corresponds to the 0th cup, the wiping assembly 140 is located at the 0th cup position, the 1st cup corresponds to the 1st cup position, and the cup positions are arranged in counterclockwise order. After resetting, the 0th and 1st cups are wiped and diluted. The cup positions are fixed on the reaction disc, and the cups are rotated. As shown in FIG. 12a, according to the reaction system, the diluent is added before the 0th rotation cycle, the sample and the reagent are added in the 2nd cycle, the diluted sample is added in the 3rd cycle, the sample is weighed and measured in the 21st cycle, the first cleaning is performed in the 24th cycle, the second cleaning is performed in the 25th cycle, and the third cleaning is performed in the 26th cycle. Therefore, n1 = 0, n2 = 2, n3 = 2, n4 = 21, and n5 = 24. As shown in FIG. 12b, the wiping position is at the 0th cup position, the sample adding position is at S, the sample weighing and measuring position is at S1, the reagent adding position is at R, and the cleaning position is at W, all of which are relative to the wiping position. Figure 4 Figure 5
[0121] As shown in FIG. 12a, the specific steps include the following steps: Figure 6
[0122] S410, before the 0th rotation cycle, according to x1 = (0 x 2) % 60 and x2 = x1 - 1, n1 = 0, x1 = 1, the 0th cup is a reaction cup, referred to as reaction cup 0, located at the 0th cup position; the 1st cup is a dilution cup, referred to as dilution cup 1, located at the 1st cup position. That is, the dilution needle is controlled to add dilution liquid to the 1st cup at the 1st cup position, and the wiping assembly 140 is controlled to wipe the 0th cup at the 0th cup position. That is, after resetting, the 0th cup is immediately wiped, and the dilution cup is added to the 1st cup. If the 0th cup is wiped and the dilution liquid is added to the 1st cup after the end of the third rotation of the 0th rotation cycle as shown in FIG. 13a, the 2nd cup should be stopped at the 0th cup position when resetting. The present application does not limit the cup positions and cup numbers, and only needs to infer according to the correspondence between the cup position number and the cup number when resetting. Figure 3
[0123] S420, after the end of the third rotation phase of the 1st rotation cycle, the reagent needle assembly 130 is controlled to suck the reagent from the reagent box, as shown in FIG. 12b. Figure 3
[0124] S430, if after reset, cup 2 is in cup 0 and cup 3 is in cup 1, then in the second rotation cycle, n2 = 2, control the sampling needle assembly 120 to pick up the original sample, such as... Figure 3 As shown in Figure 11a; after the second rotation phase of the second rotation cycle, dilution cup 1 rotates to the sample addition position S, and the sampling needle assembly 120 controls the original sample to be dispensed into the dilution cup. That is, the original sample is added to cup 1, S = ((2-0)×2-59+1)%60 = 6. That is, the original sample is added to cup 6 of the reaction plate. Also, because in the 0th cycle... Figure 3 In step 13a, the diluent has been added to dilution cup 1, and the sample dilution operation is now complete.
[0125] S440, if after reset, cup 2 is in cup position 0 and cup 3 is in cup position 1, then when n3 = 2, after the first rotation stage of the second rotation cycle, dilution cup 1 rotates to the reagent addition position R, and the reagent needle assembly 130 is controlled to add reagent to dilution cup 1, R = ((2-0)×2-41-59)%60 = 24. That is, the reagent needle assembly 130 is controlled to add reagent to dilution cup 1 at position 24 of the reaction plate.
[0126] S450, after the third rotation phase of the third rotation cycle, the sampling needle assembly 120 is positioned at the sample aspiration and weighing position to draw diluted sample from dilution cup 1. That is, the sampling needle is in... Figure 3 The action is executed at 11c. Figure 5 At position S1, a diluted sample is drawn from dilution cup 1. After drawing the diluted sample from dilution cup 1, the sampling needle assembly 120 is controlled to... Figure 3 At 11d, Figure 5 At position S, drain the diluted sample into reaction vessel 0. After this step, the sample begins to react with the reagent, which is in the second cycle. Figure 3 12a was added. Reaction data was continuously read during the reaction.
[0127] S460, in the 21st rotation cycle, n4 = 21. Before the 21st cycle, the reaction result can be calculated, and a decision can be made regarding whether to retest. If retesting is required, after the first rotation phase of the 21st cycle, dilution cup 1 is positioned at S1 on the reaction disk, and the sampling needle is controlled to draw the retest sample from dilution cup 1. That is, at this point, after the first rotation of the reaction disk assembly 110 in the 21st cycle... Figure 3 After 10a (the end of the first rotation phase of 21 cycles), the control sampling needle executes... Figure 3 Step 11a involves drawing a retest sample from dilution cup 1. The retest sample is drawn from cup 0, and S1 = ((22-0)×2+22+1)%60 = 7. That is, the retest sample is drawn from cup 7 of the reaction pan. If a retest is not required, then...Figure 3 At 11a of FIG. 1, the control unit controls the sampling needle assembly 120 to suck the original sample from the sample tube.
[0128] At S470, n5=24, after the end of the second rotation stage of the 24th rotation cycle (dilution cup 1: (24x2+22+41+1) % 60=52; reaction cup: (24x2+22+41) % 60=51), the control unit controls the washing assembly 150 to perform the first washing on the dilution cup 1, as shown in 14a of FIG. 1. Figure 3 At 14a of FIG. 1, the control unit controls the washing assembly 150 to perform the first washing on the dilution cup 1. Figure 3 At 14b of FIG. 1 (dilution cup 1: (24x2+22+41+59+1) % 60=51; reaction cup: (24x2+22+41+59) % 60=50), the control unit controls the washing assembly 150 to perform the first washing on the reaction cup 0 at the W position, as shown in 14b of FIG. 1. Figure 3 At 14b of FIG. 1, the control unit controls the washing assembly 150 to perform the first washing on the reaction cup 0. The washing position W= ((24+1-0) x2+1) % 60=51. If three washings are needed in the example, the next two cycles are performed, with one washing per cycle.
[0129] Figure 6 FIG. 1 shows a structural schematic diagram of a control device of a sample processing and scheduling system according to an embodiment of the present application. The control device of the sample processing and scheduling system includes a reaction disc control module 610, a dilution cup and reaction cup determination module 620, and a reagent and sample operation module 630.
[0130] The reaction disc control module 610 is configured to control the rotation of the reaction disc assembly 110 according to a rotation cycle, and to control the cups in the reaction disc assembly 110 to advance by a preset cup position number per rotation cycle. The rotation cycle includes multiple rotation stages, and the length of the rotation cycle and the interval length of the multiple rotation stages are determined according to a target reaction system.
[0131] The dilution cup and reaction cup determination module 620 is configured to determine a target dilution cup and a target reaction cup in the reaction disc assembly 110 according to a first target rotation cycle, the preset cup position number, and the total number of cup positions in the reaction disc assembly 110.
[0132] The reagent and sample operation module 630 is configured to control the sampling needle assembly 120 and the reagent needle assembly 130 to perform corresponding actions on the target dilution cup and the target reaction cup according to target rotation cycles corresponding to actions of sucking and injecting samples, reagents, diluents, and diluted samples in a sample processing task, so as to complete the sample processing task.
[0133] It can be understood that the device of the embodiment corresponds to the control method of the sample processing and scheduling system of the above-mentioned embodiment, and the optional items in the above-mentioned embodiment are also applicable to the embodiment, and therefore will not be described here again.
[0134] The application further provides a terminal device, which exemplarily comprises a processor and a memory, wherein the memory stores a computer program, and the processor executes the computer program to enable the terminal device to perform the functions of each module in the control method of the sample processing and scheduling system or the control device of the sample processing and scheduling system. The terminal device is a controller in the sample processing and scheduling system.
[0135] The processor can be an integrated circuit chip with a processing capability of signals. The processor can be a general processor, including a central processing unit (CPU), a graphics processing unit (GPU), and a network processor (NP), a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field programmable gate array (FPGA) or other programmable logic devices, a discrete gate or transistor logic device, a discrete hardware component, at least one of them. The general processor can be a microprocessor or the processor can be any conventional processor, etc., which can realize or execute the disclosed methods, steps and logic block diagrams in the embodiments of the application.
[0136] The memory can be, but is not limited to, a random access memory (RAM), a read only memory (ROM), a programmable read only memory (PROM), an erasable programmable read only memory (EPROM), an electrically erasable programmable read only memory (EEPROM) and the like. The memory is used to store a computer program, and the processor can execute the computer program after receiving an execution instruction.
[0137] The application further provides a computer readable storage medium for storing the computer program used in the terminal device.
[0138] In several embodiments provided in the present application, it should be understood that the disclosed apparatus and method can also be implemented by other means. The apparatus embodiments described above are only illustrative, for example, the flowcharts and structural diagrams in the drawings show the possible implementation architecture, function and operation of the apparatus, method and computer program product according to the embodiments of the present application. In this regard, each block in the flowchart or block diagram can represent a module, a program segment or a part of code, which contains one or more executable instructions for implementing the specified logical function. It should also be noted that in alternative implementation manners, the functions annotated in the blocks can also occur in an order different from that annotated in the drawings. For example, two consecutive blocks can actually be executed substantially in parallel, and sometimes they can also be executed in reverse order, depending on the functions involved. It should also be noted that each block in the structural diagram and / or flowchart, and the combination of blocks in the structural diagram and / or flowchart, can be implemented by a dedicated hardware-based system for executing the specified functions or actions, or can be implemented by a combination of dedicated hardware and computer instructions.
[0139] In addition, the functional modules or units in each embodiment of the present application can be integrated together to form an independent part, or each module can exist independently, or two or more modules can be integrated to form an independent part.
[0140] If the functions are implemented in the form of software function modules and sold or used as independent products, they can be stored in a computer readable storage medium. Based on this understanding, the technical solutions of the present application can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including a number of instructions for causing a computer device (which can be a smart phone, a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in the embodiments of the present application.
[0141] The above merely provides the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.
Claims
1. A control method of a sample processing and dispatch system, characterized by, The sample processing and scheduling system comprises a reaction disc assembly, a sampling needle assembly, a reagent needle assembly and a wiping assembly; a wiping position of the wiping assembly is fixedly arranged at a preset cup position of the reaction disc assembly; the method comprises: controlling rotation of the reaction disc assembly according to a rotation period, and each rotation period controls each cup in the reaction disc assembly to advance by a preset cup number; wherein the rotation period comprises three rotation stages; the length of the rotation period and the interval length of the three rotation stages are determined according to a target reaction system; wherein the control of the rotation of the reaction disc assembly according to the rotation period comprises: controlling the rotation of the reaction disc assembly by C1 cup positions in the first rotation stage, by C2 cup positions in the second rotation stage and by C3 cup positions in the third rotation stage in each rotation period; determining a target dilution cup and a target reaction cup in the reaction disc assembly according to a first target rotation period, the preset cup number and the total number of cup positions in the reaction disc assembly; controlling the sampling needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup according to target rotation periods of corresponding actions of sample suction and sample, reagent, diluent and diluted sample in a sample processing task, so as to complete the sample processing task, specifically comprising: after the end of the third rotation stage of the nth1 rotation period, controlling the diluent needle to add diluent to the target dilution cup, and controlling the wiping assembly to wipe the target reaction cup at the preset cup position; after the end of the second rotation stage of the nth2 rotation period, controlling the sampling needle assembly to add the original sample to the target dilution cup at a sample adding position; wherein the sample adding position is calculated according to the nth1 rotation period, the nth2 rotation period, the preset cup number, the number of cups rotated in the third rotation stage and the total number of cup positions; after the end of the first rotation stage of the nth3 rotation period, controlling the reagent needle assembly to add reagent to the target reaction cup at a reagent adding position; wherein the reagent adding position is calculated according to the nth1 rotation period, the nth3 rotation period, the preset cup number, the number of cups rotated in the second rotation stage, the number of cups rotated in the third rotation stage and the total number of cup positions; after determining retesting, after the end of the first rotation stage of the nth4 rotation period, controlling the sampling needle assembly to suck the retest used diluted sample from the target dilution cup at a retest sample suction position; after the end of the second rotation stage of the nth4 rotation period, adding the sucked retest used diluted sample to the target reaction cup; wherein the retest sample suction position is calculated according to the nth1 rotation period, the nth4 rotation period, the preset cup number, the number of cups rotated in the first rotation stage and the total number of cup positions, wherein n4>n3>n2>n1.
2. The control method of the sample processing and dispatching system according to claim 1, characterized by, The sample processing and scheduling system further comprises a cleaning assembly; the method further comprises: After the third rotation stage of the n5th rotation cycle ends, the cleaning assembly is controlled to clean the target dilution cup at a cleaning position; wherein the cleaning position is calculated according to the n1th rotation cycle, the n5th rotation cycle, the preset number of cup positions and the total number of cup positions; After the second rotation stage of the n5th rotation cycle ends, the cleaning assembly is controlled to clean the target reaction cup at the cleaning position.
3. The control method of the sample processing and dispatching system according to claim 2, wherein The cleaning position is calculated by the following formula: W = ((n5 + 1 - n1) × g + 1) % C Wherein, W is the corresponding cup position number of the cleaning position in the reaction disc assembly, n5 is the serial number of the n5th rotation cycle, n1 is the serial number of the n1th rotation cycle, g is the preset number of cup positions, and C is the total number of cup positions in the reaction disc assembly.
4. The control method of the sample processing and dispatching system according to claim 1, characterized by, The preset number of cup positions is calculated by the following formula: g = |(C1 + C2 + C3) % C| Wherein, C1 is the number of cups rotated in the first rotation stage, C2 is the number of cups rotated in the second rotation stage, C3 is the number of cups rotated in the third rotation stage, C is the total number of cup positions in the reaction disc assembly, and g is the preset number of cup positions; The sample adding position is calculated by the following formula: S = ((n2 - n1) × g + C3 + 1) % C Wherein, S is the corresponding cup position number of the sample adding position in the reaction disc assembly, n1 is the serial number of the n1th rotation cycle, and n2 is the serial number of the n2th rotation cycle; The reagent adding position is calculated by the following formula: R = ((n3 - n1) × g + C2 + C3) % C Wherein, R is the corresponding cup position number of the reagent adding position in the reaction disc assembly, and n3 is the serial number of the n3th rotation cycle; The sample weight measurement position is calculated by the following formula: S1 = ((n4 + 1 - n1) × g - C1 + 1) % C Wherein, S1 is the corresponding cup position number of the sample weight measurement position in the reaction disc assembly, and n4 is the serial number of the n4th rotation cycle.
5. The control method of the sample processing and dispatching system according to claim 4, characterized by, The target rotation cycle corresponding to the actions of sample suction and sample, reagent, diluent and diluted sample in the sample processing task is controlled to control the sample needle assembly and the reagent needle assembly to perform corresponding actions on the target dilution cup and the target reaction cup, further comprising: After the third rotation stage of the next rotation cycle of the n3th rotation cycle ends, the sample needle assembly is controlled to suck the diluted sample from the target dilution cup at the sample weight measurement position, and then the sample needle is controlled to discharge the diluted sample into the target reaction cup at the sample adding position to perform the reaction of the diluted sample and the reagent.
6. The control method of a sample processing and dispatching system according to any one of claims 1 to 5, characterized in that, The method further comprises: Before controlling the rotation of the reaction disc assembly, the reaction disc assembly is also controlled to initialize reset, and the operation of the initialization reset comprises: setting the cup number of each cup in the reaction disc assembly to be equal to the cup position number of the reaction disc assembly, and controlling the wiping assembly in the sample processing and scheduling system to be at the 0th cup position; And / or, the target dilution cup and the target reaction cup are determined in the reaction disc assembly according to the first target rotation period, the preset cup number, and the total number of cups in the reaction disc assembly, including: calculating the cup number of the target dilution cup and the target reaction cup according to the following calculation formula: x1 = (n1×g) % C x2 = x1 - 1 Wherein, x1 is the cup number of the target dilution cup; x2 is the cup number of the target reaction cup; n1 is the serial number of the first target rotation period; C is the total number of cups contained in the reaction disc assembly; g is the preset cup number.
7. A sample processing and dispatch system, characterized by, The system comprises a controller, a sampling needle assembly, a reaction disc assembly, and a reagent needle assembly; the controller implements a sample processing task by using the control method of the sample processing and scheduling system according to any one of claims 1-6.
Citation Information
Patent Citations
Sample analytical device and sample analytical method
CN101419240A
Detecting analyzer and using method thereof
CN109444444A