Methods, compositions and crystalline forms of substituted pyridone-pyridyl compounds

Abstract

The present invention relates to methods, compositions, and crystalline forms of substituted pyridone-pyridyl compounds. The present invention provides crystalline forms of methyl / fluoro-pyridyl-methoxyl substituted pyridone-pyridyl compounds of the following structure: XRPD, TGA and DSC data of # imgabs0 # crystalline form, as well as methods of making the crystalline forms, including multi-kilogram-scale preparation. Also provided are pharmaceutical compositions and methods of treating p38-mediated diseases, such as lymphoma and autoinflammatory diseases, including rheumatoid arthritis, and methods of maximizing the yield thereof, comprising the administration of a compound. Also provided is a method for maximizing the yield of the compound via a recovery procedure.

Description

[0001] This application is a divisional application of the PCT international application PCT / US2021 / 024316 filed on March 26, 2021, which entered the Chinese national phase on November 1, 2022, and the Chinese patent application number is 202180032410.2, and the invention name is "Methods, compositions and crystalline forms of substituted pyridone-pyridine compounds".

[0002] CROSS-REFERENCE TO RELATED APPLICATIONS

[0003] This application claims U.S. Provisional Patent Application Serial No. 63,000,746 filed on March 27, 2020; Serial No. 63 / 015,241 filed on April 24, 2020; Serial No. 63,018 / 954 filed on May 01, 2020; Serial No. 63 / 022,301 filed on May 08, 2020; Serial No. 63 / 022,298 filed on May 08, 2020; Serial No. 63 / 024,160 filed on May 13, 2020; Serial No. 63 / 053,903 filed on July 20, 2020; Serial No. 63 / 024,160 filed on May 13, 2020; Serial No. 63 / 053,903 filed on July 20, 2020; Serial No. 63 / 024,160 filed on May 13, 2020; Serial No. 63 / 024,160 filed on July 20, 2020; Serial No. 63 / 024,160 filed on September 10, 2020; Serial No. 63 / 024,160 filed on May 13 ... 76,689; Serial No. 63 / 126,173 filed on December 16, 2020; Serial No. 63 / 128,523 filed on December 21, 2020; Serial No. 63 / 136,080 filed on January 11, 2021; Serial No. 63 / 136,967 filed on January 13, 2021; Serial No. 63 / 138,672 filed on January 18, 202; Serial No. 63 / 140,116 filed on January 21, 2021; and the benefit of U.S. Provisional Patent Application Serial No. 63 / 149,230 filed on February 13, 2021 (each of which is incorporated herein by reference in its entirety). Technical Field

[0004] The present application relates to methods, compositions and crystalline forms of substituted pyridone-pyridyl compounds. Background Art

[0005] Atropisomers are stereoisomers arising from hindered rotation about a single bond, where the steric strain barrier to rotation is high enough to allow separation of the conformers. Oki (Oki, M; Topics in Stereochemistry 1983, 1) defines atropisomers as conformers that interconvert with a half-life of more than 1000 seconds at a given temperature. The scope of the embodiments described and claimed herein encompasses the racemic forms of the compounds as well as individual atropisomers (atropisomers that are "substantially free" of their corresponding atropisomers) and stereoisomerically enriched mixtures (i.e., mixtures of atropisomers).

[0006] Separation of atropisomers may be performed by chiral resolution methods such as selective crystallization. In atropisomer-enantioselective synthesis or atropisomer-selective synthesis, one atropisomer is formed at the expense of another atropisomer. When the isomerization reaction favors one atropisomer over the other atropisomer, atropisomer-selective synthesis may be performed by using a chiral auxiliary such as a Corey-Bakshi-Shibata (CBS) catalyst (an asymmetric catalyst derived from proline) in the total synthesis of knipholone or by methods based on thermodynamic equilibrium.

[0007] The term "atropisomerism" refers to a type of isomerism caused by hindered rotation about a single bond due to steric strain of a substituent. This phenomenon produces stereoisomers that exhibit axial chirality.

[0008] Atropisomers are generally stable but can often be in thermal equilibrium. Atropisomers will have the same but opposite optical rotations. Each atropisomer can have different properties when binding to an enzyme or receptor, with one isomer often being more potent than the other. Atropisomers are often used as pharmaceutical agents. Known examples include vancomycin and derivatives.

[0009] Pharmaceutical compositions are usually formulated with a crystalline solid of the active ingredient (API). The specific crystalline form of the API can have a significant effect on properties such as stability, dissolution rate, and bioavailability. Instability and poor solubility characteristics can limit the ability to formulate a composition with sufficient shelf life or to effectively deliver a desired amount of drug within a given dosing time frame. One strategy for achieving the desired physical parameters is to determine the most stable polymorphic form of the API and define a crystallization process that can consistently produce stable crystalline polymorphs and avoid producing alternative solid forms, mixtures of polymorphs, hydrates, or solvates that can compromise the stability and performance of the composition. Summary of the invention

[0010] The present disclosure encompasses embodiments directed to compounds of Formula (P)-Ia or derivatives thereof, wherein:

[0011] X is CH or N,

[0012] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0013] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0014] R 3 Selected from the group consisting of:

[0015]

[0016] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0017] R 5 Selected from H or C1-C3 alkyl;

[0018] m is 1 or 2;

[0019] n is 0 or 1;

[0020] p is 1; and

[0021] q is 0 or 1.

[0022] Some embodiments are directed to a pharmaceutical composition comprising a compound of Formula (P)-Ia or a derivative thereof, and a pharmaceutically acceptable carrier.

[0023] In various embodiments, the pharmaceutical compositions further include one or more additional pharmaceutically active compounds.

[0024] In other embodiments, methods for treating a condition are provided, comprising administering to a subject a therapeutically effective amount of a compound of the invention (alone or in combination with other pharmaceutically active compounds). In any embodiment, suitable conditions to be treated include, but are not limited to, autoimmune disorders, chronic inflammatory disorders, acute inflammatory disorders, autoinflammatory disorders, pain, atherosclerosis, diabetes, fibrotic diseases, metabolic disorders, cancer, neoplasia, leukemia, lymphoma, rheumatoid arthritis, and idiopathic pulmonary fibrosis.

[0025] The present disclosure also provides a method for maximizing the synthetic yield of a compound having a structure of Formula (P)-Ia by equilibration or interconversion of a compound having a structure of Formula (M)-Ib:

[0026]

[0027] The method comprises heating a solution comprising a compound having a structure of Formula (M)-Ib to a temperature at which the compound equilibrates to form a mixture of atropisomers.

[0028] The present disclosure encompasses embodiments directed to a crystalline form of Compound 49a of the following structure:

[0029]

[0030] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0031] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0032] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0033] Compound 49a.

[0034] The crystalline form of compound 49a can be characterized by one or more methods, including but not limited to X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Raman spectroscopy, infrared spectroscopy, solid state nuclear magnetic resonance, and other analytical characterization techniques known in the art.

[0035] The present disclosure includes embodiments directed to pharmaceutical compositions comprising a crystalline form of Compound 49a:

[0036]

[0037] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0038] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0039] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0040] Compound 49a,

[0041] and pharmaceutically acceptable excipients.

[0042] In some embodiments, compound 49a is the free base.

[0043] In some embodiments, the crystalline form of Compound 49a is Form A.

[0044] In some embodiments, Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78. In some embodiments, Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78 and about 15.51. In some embodiments, Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78, about 15.51, about 19.6, and about 25.92. In some embodiments, Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.48.

[0045] In some embodiments, Form A is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C, and a final sharp endothermic melting event at about 254°C.

[0046] In some embodiments, the crystalline form further comprises no more than about 20 mol% of the corresponding M isomer of compound 49a. In some embodiments, the crystalline form further comprises no more than about 0.25 mol% of the corresponding M isomer of compound 49a. In some embodiments, the crystalline form is substantially free of the corresponding M isomer of compound 49a.

[0047] In some embodiments, Compound 49a has a chemical purity of about 95% or greater.

[0048] In some embodiments, the crystalline form of compound 49a contains no more than about 20mol% of other solid forms. In some embodiments, the crystalline form of compound 49a contains no more than about 0.25mol% of other solid forms. In some embodiments, the crystalline form of compound 49a is substantially free of other solid forms.

[0049] In some embodiments, the pharmaceutical composition further comprises no more than about 20 mol% of the corresponding M isomer of compound 49a. In some embodiments, the pharmaceutical composition further comprises no more than about 0.25 mol% of the corresponding M isomer of compound 49a. In some embodiments, the pharmaceutical composition is substantially free of the corresponding M isomer of compound 49a.

[0050] In some embodiments, the therapeutically effective amount is about 10 mg to about 300 mg. In some embodiments, the therapeutically effective amount is about 50 mg.

[0051] In some embodiments, the pharmaceutical composition is an oral pharmaceutical composition.

[0052] The present disclosure includes embodiments directed to tablets comprising the pharmaceutical compositions of the present disclosure.

[0053] The present disclosure includes embodiments directed to methods of separating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib:

[0054]

[0055] in:

[0056] X is CH or N;

[0057] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0058] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0059] R 3 Selected from the group consisting of:

[0060]

[0061] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0062] R 5 Selected from H or C1-C3 alkyl;

[0063] m is 1 or 2;

[0064] n is 0 or 1;

[0065] p is 1;

[0066] q is 0 or 1;

[0067] The method comprises heating a solution comprising a compound of Formula (M)-Ib to an interconversion temperature to form a mixture of atropisomers of a compound of Formula (M)-Ib and a compound of Formula (P)-Ia.

[0068] In some embodiments, the solution is heated to the interconversion temperature for 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, or any range between two of these values. In some embodiments, the solution is heated to the interconversion temperature for 3 hours.

[0069] In some embodiments, the interconversion temperature is 110°C-210°C. In some embodiments, the interconversion temperature is 110°C-170°C. In some embodiments, the interconversion temperature is 110°C-150°C. In some embodiments, the interconversion temperature is 110°C-140°C. In some embodiments, the interconversion temperature is 110°C-125°C. In some embodiments, the interconversion temperature is 145°C-150°C.

[0070] In some embodiments, the solution further comprises a solvent that undergoes an interconversion temperature of 110°C-170°C.

[0071] In some embodiments, the solvent is aprotic. In some embodiments, the solvent is protic.

[0072] In some embodiments, the solvent is N-methylpyrrolidone, or dimethylacetamide, or a combination thereof. In some embodiments, the solvent is ethanol. In some embodiments, the solvent is ethylene glycol. In some embodiments, the solvent is n-butanol.

[0073] In some embodiments, the solvent has a boiling point of about 110°C to about 150°C.

[0074] In some embodiments, the solution including the compound of Formula (M)-Ib is heated to the boiling point of the solvent.

[0075] In some embodiments, the method of separating the compound of formula (P)-Ia by interconverting the compound of formula (M)-Ib is repeated multiple times. In some embodiments, the method of separating the compound of formula (P)-Ia by interconverting the compound of formula (M)-Ib is repeated once, twice, three times, or four times. In some embodiments, the method of separating the compound of formula (P)-Ia by interconverting the compound of formula (M)-Ib is repeated twice.

[0076] In some embodiments, the method of separating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib produces a ratio of about 2:1, about 3:1, about 4:1, about 5:1, about 6:1, about 7:1, about 8:1, about 9:1, or about 10:1 of compounds having a structure of Formula (P)-Ia substantially free of the corresponding M isomer to a compound having a structure of Formula (M)-Ib substantially free of the corresponding P isomer.

[0077] In some embodiments, the method of separating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib produces a ratio of approximately 5:1 of a compound having a structure of Formula (P)-Ia substantially free of the corresponding M isomer to a compound having a structure of Formula (M)-Ib substantially free of the corresponding P isomer.

[0078] In some embodiments, the compound of formula (P)-Ia is (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one, and the compound of formula (M)-Ib is (M)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0079] Pharmaceutical compositions are usually formulated with a crystalline solid of the active ingredient (API). The specific crystalline form of the API can have a significant effect on properties such as stability, dissolution rate, and bioavailability. Instability and poor solubility characteristics can limit the ability to formulate a composition with sufficient shelf life or to effectively deliver a desired amount of drug within a given dosing time frame. One strategy for achieving the desired physical parameters is to determine the most stable polymorphic form of the API and define a crystallization process that can consistently produce stable crystalline polymorphs and avoid producing alternative solid forms, mixtures of polymorphs, hydrates, or solvates that can compromise the stability and performance of the composition.

[0080] Some embodiments are directed to a pharmaceutical composition comprising a crystalline form of Compound 49a and a pharmaceutically acceptable carrier.

[0081] In various embodiments, the pharmaceutical composition further comprises one or more additional pharmaceutically active compounds.

[0082] In other embodiments, methods for treating a condition are provided, comprising administering to a subject a therapeutically effective amount of a crystalline form of compound 49a of the present invention (alone or in combination with other pharmaceutically active compounds). In any embodiment, suitable conditions to be treated include, but are not limited to, autoimmune disorders, chronic inflammatory disorders, acute inflammatory disorders, autoinflammatory disorders, pain, atherosclerosis, diabetes, fibrotic diseases, metabolic disorders, cancer, neoplasia, leukemia, lymphoma, rheumatoid arthritis, and idiopathic pulmonary fibrosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0083] Figure 1Illustration of the Oak Ridge Thermal Ellipsoid Program (ORTEP) plot of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one. The isomer was identified as (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0084] Figure 2 The diagram shows that the racemization of compound 49b in NMP proceeds relatively smoothly, where T 起始 ≈114°C, and racemization is complete at about 40°C below the boiling point.

[0085] Figure 3 The diagram shows that the racemization of compound 49b in ethylene glycol proceeds relatively smoothly, wherein T 起始 ≈138°C, and racemization is complete before reaching reflux.

[0086] Figure 4 Shown is an X-ray powder diffraction pattern of crystalline Form A of compound 49a showing the positions of prominent Bragg time-sharing, their 2θ positions and intensity values.

[0087] Figure 5 Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) thermogram curves of crystalline Form A of Compound 49a are shown.

[0088] Figure 6 The characteristic spectral absorbance curve of Fourier transform infrared spectroscopy (FT-IR) of crystalline Form A of compound 49a is shown, which shows the location of the significant IR active region.

[0089] Figure 7 DSC and TGA thermograms showing expanded results for the pure phase of compound 49a.

[0090] Figure 8 Shown is the arrangement of a tiny-TIM system.

[0091] Fig. 9 Cumulative crystalline Form A bioaccessibility of Compound 49a over time at 50 mg dose level under fasted and fed state conditions is shown (presented as mean ± SD).

[0092] Fig.10 Shown is the bioaccessibility of crystalline Form A of Compound 49a over time at a 50 mg dose level under fasting and fed state conditions (presenting mean ± SD).

[0093] Fig.11 Shown are the changes in the concentration of luminal crystalline Form A of compound 49a in the intestinal compartment over time at the 50 mg dose level under fasting and fed conditions (presenting mean ± SD). DETAILED DESCRIPTION

[0094] definition

[0095] Before describing the compositions and methods of the present invention, it should be understood that the present invention is not limited to the specific processes, preparations, compositions, or methods described, because these can change. It should also be understood that the terms used in the specification are only for the purpose of describing a specific type or embodiment, rather than being intended to limit the scope of the embodiments herein, and the scope of the embodiments herein will be limited only by the appended claims. Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as those generally understood by those of ordinary skill in the art. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the embodiments of the embodiments herein, preferred methods, devices, and materials are now described. All publications mentioned herein are incorporated by reference as a whole. Any content herein should not be interpreted as admitting that the embodiments herein are not entitled to be disclosed earlier than such disclosure by means of prior inventions.

[0096] It must also be noted that, as used herein and in the appended claims, the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise. Thus, for example, a reference to "p38 MAP kinase" is a reference to one or more p38 MAP kinase inhibitors known to those skilled in the art, and equivalents thereof, and so forth.

[0097] The transitional term "comprising," which is synonymous with "including," "containing," or "characterized by," is inclusive or open-ended and does not exclude additional, unrecited elements or method steps.

[0098] As used herein, the terms "consists of" or "consisting of" mean that a composition, formulation or method comprises only those elements, steps, or ingredients specifically recited in a particular claimed embodiment or claim.

[0099] As used herein, the term "consisting essentially of" or "consists essentially of" means that the composition, formulation, or method contains only the elements, steps, or ingredients specifically listed in the particular claimed embodiment or claim, and may optionally contain additional elements, steps, or ingredients that do not substantially affect the basic and novel characteristics of the particular embodiment or claim. For example, one or more of the only active ingredients in a formulation or method for treating a particular condition (e.g., nutrient depletion) are one or more therapeutic agents specifically listed in the particular embodiment or claim.

[0100] As used herein, when one embodiment is defined as being different from another embodiment, the two embodiments are "mutually exclusive." For example, an embodiment in which two groups combine to form a cycloalkyl is mutually exclusive with an embodiment in which one group is ethyl and the other group is hydrogen. Similarly, an embodiment in which one group is CH2 is mutually exclusive with an embodiment in which the same group is NH.

[0101] As used herein, the term "derivative thereof" refers to a salt thereof, a pharmaceutically acceptable salt thereof, an ester thereof, a free acid form thereof, a free base form thereof, a solvate thereof, a deuterated derivative thereof, a hydrate thereof, an N-oxide thereof, a clathrate thereof, a prodrug thereof, an isotope thereof (e.g., tritium, deuterium), or a combination thereof.

[0102] As used herein, the term "pharmaceutically acceptable salt" refers to a salt prepared from a base or acid that is acceptable for administration to a patient (such as a mammal). The term "pharmaceutically acceptable salt" includes salts that are commonly used to form alkali metal salts and to form addition salts of free acids or free bases. The nature of the salt is not critical, provided that it is pharmaceutically acceptable. Such salts can be derived from pharmaceutically acceptable inorganic or organic bases and from pharmaceutically acceptable inorganic or organic acids.

[0103] The term "inhibit" means to limit, prevent or block the action or function of a target enzyme and / or prevent, alleviate or eliminate the onset of one or more symptoms associated with a disease, condition or disorder, or to prevent, alleviate or eliminate a disease, condition or disorder.

[0104] When a numerical range is disclosed and the notation "from n1 ... to n2" or "between n1 ... and n2" (where n1 and n2 are numbers) is used, then unless otherwise specified, the notation is intended to include the numbers themselves and the range between them. The range can be integer or continuous between the end values ​​(including the end values). For example, the range "from 2 to 6 carbon atoms" is intended to include two, three, four, five, and six carbon atoms, because carbon atoms appear in integer units. In contrast, for example, the range "from 1 μM (micromolar) to 3 μM" is intended to include 1 μM, 3 μM, and any significant numbers therebetween (e.g., 1.255 μM, 2.1 μM, 2.9999 μM, etc.).

[0105] As used herein, the term "about" is intended to qualify the numerical value it modifies, thereby representing such a value as a variable within a range of error. When no specific range of error (such as the standard deviation of the mean value given in a chart or data table) is listed, the term "about" should be understood as the mean value of the numerical value of the number used with it plus or minus 10%. Therefore, about 50% means within the range of 45%-55%.

[0106] As used herein, alone or in combination, the term "acyl" refers to a carbonyl group attached to an alkenyl, alkyl, aryl, cycloalkyl, heteroaryl, heterocycle, or any other moiety wherein the atom attached to the carbonyl group is carbon. An "acetyl" group refers to a -C(O)CH3 group. An "alkylcarbonyl" group or an "alkanoyl" group refers to an alkyl group attached to the parent molecular moiety via a carbonyl group. Examples of such groups include methylcarbonyl and ethylcarbonyl. Examples of acyl groups include formyl, alkanoyl, and aroyl.

[0107] As used herein, alone or in combination, the term "alkenyl" refers to a straight or branched hydrocarbon group having one or more double bonds and containing from 2 to 20 carbon atoms. In certain embodiments, the alkenyl will include from 2 to 6 carbon atoms. The term "alkenylene" refers to a carbon-carbon double bond system connected at two or more positions, such as vinylene [(-CH=CH-), (-C::C-)]. Examples of suitable alkenyl groups include vinyl, propenyl, 2-methylpropenyl, 1,4-butadienyl, etc. Unless otherwise specified, the term "alkenyl" may include an "alkenylene" group.

[0108] As used herein, alone or in combination, the term "alkoxy" refers to an alkyl ether group, wherein the term alkyl is as defined below. Examples of suitable alkyl ether groups include methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, tert-butoxy, and the like.

[0109] As used herein, alone or in combination, the term "alkyl" refers to a straight or branched chain alkyl group containing from 1 to 20 carbon atoms. In certain embodiments, the alkyl will include from 1 to 10 carbon atoms. In other embodiments, the alkyl will include from 1 to 8 carbon atoms. Alkyl groups may be optionally substituted as defined herein.

[0110] Examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, octyl, nonyl, etc. As used herein, alone or in combination, the term "alkylene" refers to a saturated aliphatic group derived from a straight or branched saturated hydrocarbon connected at two or more positions, such as methylene (-CH2-). Unless otherwise specified, the term "alkyl" may include an "alkylene" group.

[0111] As used herein, alone or in combination, the term "alkylamino" refers to an alkyl group attached to the parent molecular moiety through an amino group. Suitable alkylamino groups can be monoalkylated or dialkylated groups such as, for example, N-methylamino, N-ethylamino, N,N-dimethylamino, N,N-ethylmethylamino, etc.

[0112]

[0046] The term "alkylidene," as used herein, alone or in combination, refers to an alkenyl group wherein one carbon atom of the carbon-carbon double bond belongs to the portion to which the alkenyl group is attached.

[0113] As used herein, alone or in combination, the term "alkylthio" refers to an alkylthioether (RS-) group, wherein the term alkyl is as defined above, and wherein the sulfur may be singly or doubly oxidized. Examples of suitable alkylthioether groups include methylthio, ethylthio, n-propylthio, isopropylthio, n-butylthio, isobutylthio, sec-butylthio, tert-butylthio, methanesulfinyl, ethanesulfinyl, and the like.

[0114] As used herein, alone or in combination, the term "alkynyl" refers to a straight or branched hydrocarbon group having one or more triple bonds and containing from 2 to 20 carbon atoms. In certain embodiments, the alkynyl includes from 2 to 6 carbon atoms. In other embodiments, the alkynyl includes from 2 to 4 carbon atoms. The term "alkynylene" refers to a carbon-carbon triple bond connected at two positions, such as ethynylene (-C:::C-, -C≡C-).

[0115] Examples of alkynyl groups include ethynyl, propynyl, hydroxypropynyl, butyn-1-yl, butyn-2-yl, pentyn-1-yl, 3-methylbutyn-1-yl, hexyn-2-yl, etc. Unless otherwise specified, the term "alkynyl" may include "alkynylene" groups.

[0116] As used herein, alone or in combination, the terms "amido" and "carbamoyl" refer to an amino group attached to a parent molecular moiety via a carbonyl group as described below, or vice versa. As used herein, alone or in combination, the term "C-amido" refers to a -C(O)N(RR') group, wherein R and R' are as defined herein or as defined by a specific enumerated "R" group as specified. As used herein, alone or in combination, the term "N-amido" refers to a RC(O)N(R')- group, wherein R and R' are as defined herein or as defined by a specific enumerated "R" group as specified. As used herein, alone or in combination, the term "amido" includes an acyl group attached to a parent moiety via an amino group. An example of an "amido" group is acetamido (CH3C(O)NH-).

[0117] As used herein, alone or in combination, the term "amino" refers to -NRR', wherein R and R' are independently selected from hydrogen, alkyl, acyl, heteroalkyl, aryl, cycloalkyl, heteroaryl, and heterocycloalkyl, any of which may itself be optionally substituted. In addition, R and R' may combine to form heterocycloalkyl, any of which may be optionally substituted.

[0118] As used herein, alone or in combination, the term "aryl" means a carbocyclic aromatic system containing one, two or three rings, wherein such polycyclic ring systems are fused together. The term "aryl" includes aromatic groups such as phenyl, naphthyl, anthracenyl, and phenanthrenyl.

[0119]

[0063] The term "arylalkenyl" or "aralkenyl," as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an alkenyl group.

[0120]

[0063] The term "arylalkoxy" or "aralkoxy," as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an alkoxy group.

[0121]

[0043] The term "arylalkyl" or "aralkyl," as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an alkyl group.

[0122]

[0063] The term "arylalkynyl" or "aralkynyl," as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an alkynyl group.

[0123] The term "arylalkanoyl" or "aralkanoyl" or "aroyl" as used herein, alone or in combination, refers to an acyl group derived from an aryl-substituted alkanecarboxylic acid (e.g., benzoyl, naphthoyl, phenylacetyl, 3-phenylpropionyl(hydrocinnamoyl), 4-phenylbutyroyl, (2-naphthyl)acetyl, 4-chlorohydrocinnamoyl, and the like).

[0124] The term aryloxy, as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an oxy group.

[0125] As used herein, alone or in combination, the terms "benzo" and "benz" refer to a divalent radical C6H4= derived from benzene. Examples include benzothiophene and benzimidazole.

[0126] The term "carbamate," as used herein, alone or in combination, refers to an ester of a carbamic acid (-NHCOO-), which may be attached to the parent molecular moiety from either the nitrogen terminus or the acid terminus, and which may be optionally substituted as defined herein.

[0127] The term "O-carbamyl," as used herein, alone or in combination, refers to a -OC(O)NRR' group, with R and R' as defined herein.

[0128] The term "N-carbamyl," as used herein, alone or in combination, refers to a ROC(O)NR'- group, with R and R' as defined herein.

[0129] As used herein, the term "carbonyl" when used alone encompasses the formyl group [-C(O)H] and when used in combination, the term "carbonyl" is a -C(O)- group.

[0130] As used herein, the term "carboxyl" or "carboxy" refers to -C(O)OH or the corresponding "carboxylate" anion (e.g., in carboxylates). An "O-carboxyl" group refers to a RC(O)O- group, where R is as defined herein. A "C-carboxyl" group refers to a -C(O)OR group, where R is as defined herein.

[0131] The term "cyano," as used herein, alone or in combination, refers to -CN.

[0132] As used herein individually or in combination, the term "cycloalkyl" or alternatively "carbocycle" refers to a saturated or partially saturated monocyclic, bicyclic or tricyclic alkyl group, wherein each ring portion contains from 3 to 12 carbon atom ring members, and can be alternatively a benzo-fused ring system optionally substituted as defined herein. In certain embodiments, the cycloalkyl will include from 5 to 7 carbon atoms. Examples of such cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, tetrahydronaphthyl, indanyl, octahydronaphthyl, 2,3-dihydro-1H-indenyl, adamantyl, etc. As used herein, "bicyclic" and "tricyclic" are intended to include fused ring systems, such as decalin, octahydronaphthalene, and polycyclic (polycentric) saturated or partially unsaturated types. The latter type of isomers are typically exemplified by bicyclo[1,1,1]pentane, camphor, adamantane, and bicyclo[3,2,1]octane.

[0133]

[0063] The term "ester," as used herein, alone or in combination, refers to a carboxyl group bridging two moieties linked at carbon atoms.

[0134]

[0063] The term "ether," as used herein, alone or in combination, refers to an oxy group that bridges two moieties connected at carbon atoms.

[0135]

[0043] The term "halogen" or "halogen element" as used herein, alone or in combination, refers to fluorine, chlorine, bromine, or iodine.

[0136] The term "haloalkoxy," as used herein, alone or in combination, refers to a haloalkyl group attached to the parent molecular moiety through an oxygen atom.

[0137] As used herein individually or in combination, the term "haloalkyl" refers to an alkyl group with the meaning as defined above, in which one or more hydrogens are replaced by halogen elements. Specifically comprising monohaloalkyl groups, dihaloalkyl groups and polyhaloalkyl groups. For example, monohaloalkyl groups can have iodine atoms, bromine atoms, chlorine atoms or fluorine atoms in the group. Dihaloalkyl groups and polyhaloalkyl groups can have a combination of two or more identical halogen atoms or different halogen groups. The example of a haloalkyl group includes fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl, pentafluoroethyl, heptafluoropropyl, difluorochloromethyl, dichlorofluoromethyl, difluoroethyl, difluoropropyl, dichloroethyl and dichloropropyl. "Haloalkylene" refers to a haloalkyl group connected at two or more positions. Examples include fluoromethylene (-CFH-), difluoromethylene (-CF2-), chloromethylene (-CHCl-) etc.

[0138] As used herein, individually or in combination, the term "heteroalkyl" refers to a stable straight or branched chain, or a combination thereof, fully saturated or containing from 1 to 3 degrees of unsaturation, consisting of a specified number of carbon atoms and one to three heteroatoms selected from N, O, and S, and wherein the N and S atoms may be optionally oxidized, and the N heteroatom may be optionally quaternized. One or more heteroatoms may be located at any interior position of the heteroalkyl group. Up to two heteroatoms may be consecutive (such as, for example, -CH2-NH-OCH3).

[0139] As used herein, alone or in combination, the term "heteroaryl" refers to a 5- to 15-membered unsaturated heteromonocyclic ring, or a fused monocyclic ring system, a bicyclic ring system, or a tricyclic ring system, wherein at least one of the fused rings is aromatic, containing at least one atom selected from N, O, and S. In certain embodiments, the heteroaryl will include from 1 to 4 heteroatoms as ring members. In other embodiments, the heteroaryl will include from 1 to 2 heteroatoms as ring members. In certain embodiments, the heteroaryl will include from 5 to 7 atoms. The term also includes fused polycyclic groups, wherein the heterocycle is fused to an aryl ring, wherein the heteroaryl ring is fused to other heteroaryl rings, wherein the heteroaryl ring is fused to a heterocycloalkyl ring, or wherein the heteroaryl ring is fused to a cycloalkyl ring. Examples of heteroaryl groups include pyrrolyl, pyrrolinyl, imidazolyl, pyrazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazolyl, pyranyl, furanyl, thienyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, thiadiazolyl, isothiazolyl, indolyl, isoindolyl, indolizinyl, benzimidazolyl, quinolyl, isoquinolyl, quinoxalinyl, quinazolinyl, indazolyl, benzotriazole, The invention also includes but is not limited to benzophenone, benzothiophene ...

[0140] As used herein individually or in combination, the term "heterocycloalkyl" and interchangeable "heterocycle" each refer to a saturated, partially unsaturated, or completely unsaturated (but non-aromatic) monocyclic, bicyclic, or tricyclic heterocyclic group containing at least one heteroatom as a ring member, wherein each of the heteroatoms can be independently selected from nitrogen, oxygen, and sulfur. In certain embodiments, heterocycloalkyl will include from 1 to 4 heteroatoms as ring members. In other embodiments, the heterocycloalkyl will include from 1 to 2 heteroatoms as ring members. In certain embodiments, the heterocycloalkyl will include from 3 to 8 ring members in each ring. In other embodiments, the heterocycloalkyl will include from 3 to 7 ring members in each ring. In yet other embodiments, the heterocycloalkyl will include from 5 to 6 ring members in each ring. "Heterocycloalkyl" and "heterocycle" are intended to include sulfones, sulfoxides, N-oxides having a tertiary nitrogen ring member, and carbocyclic fused ring systems and benzofused ring systems; in addition, both terms also include systems in which a heterocycle is fused to an aryl group as defined herein or to an additional heterocyclic group. Examples of heterocyclic groups include aziridinyl, azetidinyl, 1,3-benzodioxolanyl, dihydroisoindolyl, dihydroisoquinolinyl, dihydrocinnolinyl, dihydrobenzodioxinyl, dihydro[1,3]oxazolo[4,5-b]pyridinyl, benzothiazolyl, dihydroindolyl, dihydropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, isoindolyl, morpholinyl, piperazinyl, pyrrolidinyl, tetrahydropyridinyl, piperidinyl, thiomorpholinyl, and the like. Unless explicitly prohibited, heterocyclic groups may be optionally substituted.

[0141] The term "hydrazino," as used herein, alone or in combination, refers to two amino groups connected by a single bond (ie, -NN-).

[0142] The term "hydroxy," as used herein, alone or in combination, refers to -OH.

[0143] The term "hydroxyalkyl," as used herein, alone or in combination, refers to a hydroxy group attached to the parent molecular moiety through an alkyl group.

[0144] The term "imino," as used herein, alone or in combination, refers to =N-.

[0145] The term "iminohydroxy," as used herein, alone or in combination, refers to =N(OH) and =NO-.

[0146] The phrase "in the backbone" refers to the longest continuous or adjacent chain of carbon atoms starting at the point of attachment of a group to the compound having any of the formulae disclosed herein.

[0147] The term "isocyanato" refers to an -NCO group.

[0148] The term "isothiocyanato" refers to a -NCS group.

[0149] The phrase "linear chain of atoms" refers to the longest straight chain of atoms independently selected from the group consisting of carbon, nitrogen, oxygen, and sulfur.

[0150] As used herein, alone or in combination, unless specifically defined otherwise, the term "lower" means containing from 1 to 6 carbon atoms (including 6 carbon atoms) (ie, C1-C6 alkyl).

[0151] The term "lower aryl" as used herein, alone or in combination, means phenyl or naphthyl, either of which may be optionally substituted as specified.

[0152] As used herein, the term "lower heteroaryl" alone or in combination means: 1) a monocyclic heteroaryl comprising five or six ring members, wherein between one and four of the ring members may be heteroatoms selected from N, O, and S; or 2) a bicyclic heteroaryl wherein each of the fused rings comprises five or six ring members, wherein between the ring members there are one to four heteroatoms selected from N, O, and S.

[0153] As used herein, alone or in combination, the term "lower cycloalkyl" means a monocyclic cycloalkyl having between three and six ring members (i.e., C3-C6 cycloalkyl). Lower cycloalkyl can be unsaturated. Examples of lower cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

[0154] As used herein, alone or in combination, the term "lower heterocycloalkyl" means a monocyclic heterocycloalkyl having between three and six ring members, one and four of which may be heteroatoms selected from N, O, and S (i.e., C3-C6 heterocycloalkyl). Examples of lower heterocycloalkyls include pyrrolidinyl, imidazolidinyl, pyrazolidinyl, piperidinyl, piperazinyl, and morpholinyl. Lower heterocycloalkyls may be unsaturated.

[0155] The term "lower amino" as used herein, alone or in combination, refers to -NRR', wherein R and R' are independently selected from hydrogen and lower alkyl, either of which may be optionally substituted.

[0156] The term "thiol," as used herein, alone or in combination, refers to an RS- group, with R as defined herein.

[0157] The term "nitro," as used herein, alone or in combination, refers to -NO2.

[0158] As used herein, "N-oxides" are formed from tertiary basic amines or imines present in the molecule using a convenient oxidizing agent.

[0159]

[0063] The term "oxy" or "oxa," as used herein, alone or in combination, refers to -O-.

[0160] The term "oxo," as used herein, alone or in combination, refers to =0.

[0161] The term "perhaloalkoxy" refers to an alkoxy group in which all of the hydrogen atoms are replaced by halogen atoms.

[0162] The term "perhaloalkyl," as used herein, alone or in combination, refers to an alkyl group in which all of the hydrogen atoms are replaced with halogen atoms.

[0163] Or as used herein, alone or in combination, and used interchangeably with the term "substantially pure", the term "substantially free" refers to a compound that is free of all other compounds within the detection limit measured by any means, including nuclear magnetic resonance (NMR), gas chromatography / mass spectrometry (GC / MS), or liquid chromatography / mass spectrometry (LC / MS). In embodiments, substantially free can be less than about 1.0%, less than about 0.5%, less than about 0.4%, less than about 0.3%, less than about 0.2%, less than about 0.1%, less than about 0.05%, or less than about 0.01%.

[0164] As used herein, the terms "sulfonate," "sulfonic acid," and "sulfonic," alone or in combination, refer to a -SO3H group, and are used in salt form as the anion of the sulfonic acid.

[0165] The term "sulfanyl," as used herein, alone or in combination, refers to -S-.

[0166] The term "sulfinyl," as used herein, alone or in combination, refers to -S(O)-.

[0167] The term "sulfonyl," as used herein, alone or in combination, refers to -S(O)2-.

[0168] The term "N-sulfonamido" refers to an RS(=0)2NR'- group, where R and R' are as defined herein.

[0169] The term "S-sulfonamido" refers to a -S(=0)2NRR' group, where R and R' are as defined herein.

[0170] As used herein, alone or in combination, the terms "thia" and "thio" refer to an -S- group or an ether in which the oxygen is replaced by sulfur. The oxidized derivatives of the thio group (ie, sulfinyl and sulfonyl) are included in the definition of thia and thio.

[0171] The term "thiol" as used herein, alone or in combination, refers to a -SH group.

[0172] As used herein, the term "thiocarbonyl" when used alone encompasses the thioformyl group -C(S)H and when used in combination, the term "thiocarbonyl" is a -C(S)- group.

[0173] The term "N-thiocarbamyl" refers to a ROC(S)NR'- group, where R and R' are as defined herein.

[0174] The term "O-thiocarbamyl" refers to a -OC(S)NRR' group, where R and R' are as defined herein.

[0175] The term "thiocyanato" refers to a -CNS group.

[0176] The term "trihalomethanesulfonamido" refers to a X3CS(O)2NR- group, where X is a halogen element and R is as defined herein.

[0177] The term "trihalomethanesulfinyl" refers to a X3CS(O)2- group, where X is a halogen element.

[0178] The term "trihalomethoxy" refers to a X3CO- group, where X is a halogen element.

[0179] As used herein, alone or in combination, the term "trisubstituted silyl" refers to a silicone group substituted at its three free valencies by groups as listed herein under the definition of substituted amino. Examples include trimethylsilyl, tert-butyldimethylsilyl, triphenylsilyl, and the like.

[0180] The term "3,5-difluoropyridin-2-yl" refers to a moiety of the following structure:

[0181]

[0182] The term "3-fluoropyridin-2-yl" refers to a moiety of the following structure:

[0183]

[0184] The term "5-fluoro-3-methylpyridin-2-yl" refers to a moiety of the following structure:

[0185]

[0186] The term "6-fluoropyridin-2-yl" refers to a moiety of the following structure:

[0187]

[0188] The term "6-fluoro-4-methylpyridin-2-yl" refers to a moiety of the following structure:

[0189]

[0190] The term "3-fluoro-5-methylpyridin-2-yl" refers to a moiety of the following structure:

[0191]

[0192] The term "5-fluoropyridin-2-yl" refers to a moiety of the following structure:

[0193]

[0194] The term "4-fluoropyridin-3-yl" refers to a moiety of the following structure:

[0195]

[0196] The term "5-fluoropyrimidin-4-yl" refers to a moiety of the following structure:

[0197]

[0198] Any definition herein may be used in combination with any other definition to describe a composite structural group. By convention, the trailing element of any such definition is the element connected to the parent moiety. For example, the composite group alkylamido represents an alkyl group connected to the parent molecule via an amide group, and the term alkoxyalkyl represents an alkoxy group connected to the parent molecule via an alkyl group.

[0199] When a group is defined as "null", it means that the group does not exist.

[0200] The term "optionally substituted" means that the preceding group may be substituted or unsubstituted. When substituted, the substituent of the "optionally substituted" group may include (but not limited to) one or more substituents independently selected from the following groups or specifically designated groups (alone or in combination): lower alkyl, lower alkenyl, lower alkynyl, lower alkanoyl, lower heteroalkyl, lower heterocycloalkyl, lower haloalkyl, lower haloalkenyl, lower haloalkynyl, lower perhaloalkyl, lower perhaloalkoxy, lower cycloalkyl, phenyl, aryl, aryloxy, lower alkoxy, lower haloalkoxy, oxo, Lower acyloxy, carbonyl, carboxyl, lower alkyl carbonyl, lower carboxylate, lower formamide, cyano, hydrogen, halogen, hydroxyl, amino, lower alkyl amino, aryl amino, amide, nitro, thiol, lower alkyl thio, lower haloalkyl thio, lower perhaloalkyl thio, aryl thio, sulfonic acid, sulfonic acid, trisubstituted silyl, N3, SH, SCH3, C(O)CH3, CO2CH3, CO2H, pyridyl, thiophene, furyl, lower carbamate, and lower urea. Where structurally feasible, two substituents may be linked together to form a fused five-membered, six-membered, or seven-membered carbocyclic or heterocyclic ring consisting of zero to three heteroatoms, such as forming methylenedioxy or ethylenedioxy. The optionally substituted group may be unsubstituted (e.g., -CH2CH3), completely substituted (e.g., -CF2CF3), monosubstituted (e.g., -CH2CH2F), or substituted at any level between completely substituted and monosubstituted (e.g., -CH2CF3). Where substituents are listed but not characterized as substituted, both substituted and unsubstituted forms are included. Where substituents are characterized as "substituted", substituted forms are particularly intended. In addition, different groups of optional substituents for a particular moiety may be defined as desired; in these cases, the optional substitution will typically be defined immediately following the phrase as "optionally substituted by...".

[0201] Unless otherwise defined, the term R or the term R' refers to a portion selected from hydrogen, alkyl, cycloalkyl, heteroalkyl, aryl, heteroaryl and heterocycloalkyl (any of which can be optionally substituted) when it appears alone and without a numerical designation. Such R and R' groups should be understood to be optionally substituted as defined herein. Whether or not the R group has a numerical designation, it should be understood that each R group (including R, R' and Rn (where n = (1, 2, 3, ... n)), each substituent and each term are independent of each other in terms of selection from the group. If any variable, substituent, or term (e.g., aryl, heterocycle, R, etc.) appears more than once in a formula or family structure, its definition at each occurrence is independent of the definition at each other occurrence. Those skilled in the art will also recognize that certain groups can be connected to the parent molecule or can occupy a position in the chain of an element from either end as written. For example, an asymmetric group (such as -C (O) N (R) -) can be connected to the parent part at carbon or nitrogen.

[0202] Asymmetric centers are present in the compounds disclosed herein. These centers are designated by the symbol "R" or "S", depending on the configuration of the substituents around the chiral carbon atom. It should be understood that the present invention includes all stereochemical isomeric forms (including diastereomeric forms, enantiomeric forms and epimeric forms, as well as d-isomers and l-isomers, and mixtures thereof). Individual stereoisomers of compounds can be prepared synthetically from commercially available starting materials containing chiral centers, or by preparing a mixture of enantiomeric products, followed by separation, such as conversion into a mixture of diastereoisomers, followed by separation or recrystallization, chromatography, by direct separation of enantiomers on a chiral chromatographic column, or any other suitable method known in the art. The starting compound of a specific stereochemistry is commercially available or can be manufactured and split by techniques known in the art. In addition, compounds disclosed herein can exist as geometric isomers. The present invention includes all cis isomers, trans isomers, isomers, retro isomers, cis (E) isomers, and hetero (Z) isomers, and appropriate mixtures thereof. In addition, compounds may exist as tautomers; the present invention provides all tautomeric isomers. In addition, the compounds disclosed herein may exist in a non-solvated form, as well as in a solvated form with a pharmaceutically acceptable solvent (such as water, ethanol, etc.). Generally, the solvated form is considered to be equivalent to the non-solvated form.

[0203] When atoms connected by a bond are considered to be part of a larger substructure, the term "bond" refers to a covalent bond between two atoms, or two moieties. Unless otherwise specified, a bond can be a single bond, a double bond, or a triple bond. A dotted line between two atoms in a molecular diagram indicates that an additional bond may or may not be present at that position.

[0204] As used herein, the term "disease" is intended to be generally synonymous with and used interchangeably with the terms "disorder," "symptom," and "condition" (in the medical context), as all reflect an abnormal condition of the human or animal body, or one of its parts, which impairs normal function, is usually manifested by distinctive signs and symptoms, and causes the human or animal to have a reduced lifespan or quality of life.

[0205] The term "combination therapy" means the administration of two or more therapeutic agents to treat the conditions or disorders described in the present disclosure. Such administration comprises co-administering these therapeutic agents in a substantially simultaneous manner, such as in a single capsule with a fixed ratio of active ingredients, or in multiple separate capsules of each active ingredient. In addition, such administration also comprises the use of each type of therapeutic agent in a sequential manner. In either case, the treatment regimen will provide a beneficial effect of the drug combination in treating the conditions or disorders described herein.

[0206] "P38 MAP kinase inhibitor" is used herein to refer to an inhibitor that exhibits an IC for p38 MAP kinase activity of no more than about 100 μM and more typically no more than about 50 μM. 50 Compounds that have an IC of 1. 50 The concentration of an inhibitor that reduces the activity of an enzyme (e.g., p38 MAP kinase) to half-maximal levels. It has been found that certain compounds disclosed herein exhibit inhibition against p38 MAP kinase. In some embodiments, the compound will exhibit an IC of no more than about 1 nM against p38 MAP kinase. 50 In some embodiments, the compound will exhibit an IC against p38 MAP kinase of no more than about 1 μM. 50 In some embodiments, the compound will exhibit an IC against p38 MAP kinase of about 1 μM to about 50 μM. 50 In certain embodiments, the compound will exhibit an IC against p38 MAP kinase of no more than about 10 μM. 50 In further embodiments, the compound will exhibit an IC against p38 MAP kinase of no more than about 5 μM 50 In yet further embodiments, the compound will exhibit an IC against p38 MAP kinase of no more than about 1 μM 50 In yet further embodiments, the compound will exhibit an IC against p38 MAP kinase of no more than about 300 nM 50 (as measured in the p38 MAP kinase assay described herein).

[0207] The phrase "therapeutically effective" is intended to qualify the amount of active ingredient used for the treatment of a disease or disorder, or the effect of a clinical endpoint.

[0208] As used herein, the term "therapeutic" or "therapeutic agent" or "pharmaceutically active agent" means an agent used to treat, combat, modify, prevent or ameliorate an undesirable condition or disease in a patient. In part, embodiments of the present invention are directed to the treatment of p38 MAP kinase-mediated diseases.

[0209] The "therapeutically effective amount" or "effective amount" of the composition is a predetermined amount calculated to achieve the desired effect (e.g., inhibition, retardation, reversal of cell activation, migration, or proliferation). The activity expected by the present method includes (as the case may be) both medical therapeutic treatment and / or the treatment of preventive diseases. The specific dosage of the compound administered according to the present invention to obtain a therapeutic effect and / or a preventive effect will of course be determined by the specific circumstances surrounding the case, including, for example, the compound administered, the route of administration, and the condition being treated. The compound is effective over a wide dosage range, and for example, the daily dosage will generally be in the range of 0.001 mg / kg to 10 mg / kg, more generally in the range of 0.01 mg / kg to 5 mg / kg. However, it will be understood that the effective amount administered will be determined by the physician according to the relevant circumstances (including the condition to be treated, the selection of the compound to be administered, and the selected route of administration), and therefore, the above dosage range is not intended to limit the scope of the present invention in any way. A therapeutically effective amount of a compound of the invention is typically an amount sufficient to achieve an effective systemic or local concentration in a tissue when the compound is administered in a physiologically tolerable excipient composition.

[0210] The term "therapeutically acceptable" refers to those compounds and derivatives thereof that are suitable for contact with the tissues of patients without excessive toxicity, irritation and allergic response, commensurate with a reasonable benefit / risk ratio, and effective for their intended use.

[0211] As used herein, the terms "treat," "treated," "treating," or "treatment" refer to both therapeutic treatment and / or prophylactic measures or preventative measures, wherein the object is to prevent or slow down (mitigate) an undesirable physiological condition, disorder, or disease, or to obtain a beneficial or desired clinical result. For purposes of the present invention, beneficial or desired clinical results include, but are not limited to, alleviation of symptoms; attenuation of the extent of the condition, disorder, or disease; stabilization of the state of the condition, disorder, or disease (i.e., not worsening); delay of the onset of the condition, disorder, or disease or slowing of the progression of the condition, disorder, or disease; alleviation of the condition, disorder, or disease state; and remission (whether partial or total, whether induced or maintained), whether detectable or undetectable, of the condition, disorder, or disease, or enhancement or improvement of the condition, disorder, or disease. Treatment includes inducing a clinically significant response without excessive levels of side effects. Treatment also includes prolonging survival compared to expected survival if not receiving treatment. Treatment can also be preemptive in nature, that is, it can comprise the prevention of disease.The prevention of disease can be related to being free from disease completely (for example, as in the case of preventing pathogen infection), or can be related to the prevention of disease progression.For example, the prevention of disease may not mean the complete elimination of any impact relevant to the disease at any level, but may mean that the symptoms of disease are prevented to clinically significant or detectable levels.The prevention of disease can also mean preventing disease progression to late stage of disease, and prolonging disease-free survival compared with untreated disease-free survival, and prolonging disease-free survival compared with untreated disease-free survival.

[0212] When used in conjunction with a treatment, "administering" means administering a therapeutic agent directly into or onto a target tissue, or administering a therapeutic agent to a patient, whereby the therapeutic agent positively affects the tissue to which it is targeted. Therefore, as used herein, when used in conjunction with the compounds of the embodiments herein, the term "administering" may include (but is not limited to): providing a compound into or onto a target tissue; providing a compound systemically to a patient, such as by intravenous injection, whereby the therapeutic agent reaches the target tissue; providing a compound in a form encoding its sequence to the target tissue (e.g., by so-called gene therapy techniques). "Administering" a composition may be achieved by injection, topically, orally, or by any of these methods in combination with other known techniques.

[0213] The term "patient" is generally synonymous with the term "subject" and includes all mammals (including humans). Examples of patients include humans, livestock (such as cattle, goats, sheep, pigs, and rabbits), and companion animals (such as dogs, cats, rabbits, and horses). Preferably, the patient is a human.

[0214] As used herein, the terms "excipient" and "pharmaceutically acceptable excipient" are intended to be generally synonymous with the terms "carrier," "pharmaceutically acceptable carrier," "diluent," "pharmaceutically acceptable diluent," and may be used interchangeably.

[0215] The term "prodrug" refers to a compound that becomes more active in vivo. Certain compounds disclosed herein may also exist as prodrugs, as described in Hydrolysis in Drug and Prodrug Metabolism: Chemistry, Biochemistry, and Enzymology (Testa, Bernard and Mayer, Joachim M. Wiley-VHCA, Zurich, Switzerland 2003). The prodrug of the compound described herein is a structurally modified form of a compound that is easily subjected to chemical changes under physiological conditions and provides a compound. In addition, the prodrug can be converted into a compound by a chemical method or a biochemical method in an ex vivo environment. For example, when the prodrug is placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent, the prodrug can be slowly converted into a compound. Prodrugs are generally useful because in some cases, prodrugs can be easier to apply than compounds or parent drugs. For example, prodrugs can be bioavailable by oral administration, while parent drugs are not. Compared with parent drugs, prodrugs can also have improved solubility in pharmaceutical compositions. A variety of prodrug derivatives are known in the art, such as those that rely on hydrolytic cleavage or oxidative activation of the prodrug. Examples of prodrugs (not limited to) are compounds that are administered as esters ("prodrugs") but are subsequently metabolically hydrolyzed to carboxylic acids (active entities). Additional examples include peptidyl derivatives of compounds.

[0216] As used herein, the term "therapeutically acceptable salt" means a salt or zwitterionic form of a compound disclosed herein, which is water-soluble or oil-soluble or dispersible and as defined herein for therapeutically acceptable. Salt can be prepared during the final separation and purification of the compound, or prepared individually by reacting the appropriate compound of the free alkali form with a suitable acid. Representative acid addition salts include acetate, adipate, alginate, L-ascorbate, aspartate, benzoate, benzenesulfonate (benzenesulfonate) (benzenesulfonate (besylate)), bisulfate, butyrate, camphorate, camphorsulfonate, citrate, digluconate, formate, fumarate, gentisate, glutarate, glycerophosphate, glycolate, hemisulfate, enanthate, caproate, hippurate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethylsulfonate (esylate), lactate, maleic acid The compounds disclosed herein can be quaternized with the following: methyl, ethyl, propyl and butyl chlorides, bromides, and iodides; dimethyl, diethyl, dibutyl and diamyl sulfides; decyl, lauryl, myristyl and steryl chlorides, bromides, and iodides; and benzyl and phenethyl bromides. Examples of acids that can be used to form therapeutically acceptable addition salts include inorganic acids (such as hydrochloric acid, hydrobromic acid, sulfuric acid, and phosphoric acid) and organic acids (such as oxalic acid, maleic acid, succinic acid, and citric acid). Salts can also be formed by coordination of the compound with an alkali metal or alkaline earth ion. Thus, the present invention contemplates sodium salts, potassium salts, magnesium salts, and calcium salts of the compounds disclosed herein, and the like.

[0217] List of abbreviations:

[0218] ACN Acetonitrile

[0219] Boc tert-Butyloxycarbonyl

[0220] Bu Butyl

[0221] Bpy 2,2'-Bipyridine

[0222] DCA Dichloroacetic acid

[0223] DCI Dicyclohexylcarbodiimide

[0224] DCM Dichloromethane or Methylene Chloride

[0225] DIPEA Diisopropylethylamine

[0226] DMA Dimethylacetamide

[0227] DMAP 4-dimethylaminopyridine or N,N-dimethylaminopyridine

[0228] DME 1,2-Dimethoxyethane

[0229] DMF N,N-Dimethylformamide

[0230] DMSO Dimethyl sulfoxide

[0231] CuBr2 Copper(II) Bromide

[0232] EDAC N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride

[0233] eq. equivalent

[0234] Et Ethyl

[0235] EtOAC Ethyl acetate

[0236] EtOH

[0237] HPLC High Pressure Liquid Chromatography

[0238] h one or more hours

[0239] IPA Isopropyl Alcohol

[0240] K2CO3 Potassium carbonate

[0241] KOtBu Potassium tert-butoxide

[0242] LAH Lithium Aluminum Hydride

[0243] LC / MS Liquid Chromatography Mass Spectrometry

[0244] LC / MS / MS Liquid chromatography tandem mass spectrometry

[0245] mCPBA Meta-chloroperbenzoic acid

[0246] Me Methyl

[0247] MeCN Acetonitrile

[0248] MeOH Methanol

[0249] MgSO4 Magnesium sulfate

[0250] mL milliliters

[0251] mmol millimole

[0252] NaH Sodium hydride

[0253] NaN(TMS)2 Sodium bis(trimethylsilyl)amide

[0254] NCS N-Chlorosuccinimide

[0255] NMR Nuclear Magnetic Resonance

[0256] NMP N-Methylpyrrolidone

[0257] Pd / C Palladium Carbon

[0258] Ph Phenyl

[0259] PPA Polyphosphoric acid

[0260] TEA Triethylamine

[0261] TFA Trifluoroacetic acid

[0262] THF Tetrahydrofuran

[0263] TOSMIC Tosylmethyl isocyanide

[0264] TSA p-Toluenesulfonic acid.

[0265] Compounds and crystalline forms of compounds

[0266] Embodiments herein are directed to compounds, crystalline forms of compounds, and pharmaceutical compositions, some of which have been found to inhibit p38 MAP kinase, and methods of synthesizing and using the compounds. Some embodiments include methods for treating a disease in a patient by administering thereto the compounds, crystalline forms of compounds, and pharmaceutical compositions described herein.

[0267] Certain compounds and crystalline forms of compounds disclosed herein may have useful p38MAP kinase inhibitory activity and may be used to treat or prevent diseases or conditions in which p38MAP kinase plays an active role. Therefore, embodiments also provide pharmaceutical compositions comprising one or more compounds disclosed herein and a pharmaceutically acceptable carrier, as well as methods of making and using compounds and compositions. Certain embodiments provide methods of inhibiting p38MAP kinase using compounds of the embodiments herein. Other embodiments provide methods for treating p38MAP kinase-mediated disorders in patients in need of such treatment, the methods comprising administering to the patient a therapeutically effective amount of a compound or composition according to the present disclosure. Also provided is the use of certain compounds disclosed herein in the manufacture of a medicament for treating a disease or condition improved by inhibition of p38MAP kinase.

[0268] Embodiments are also provided wherein any embodiment herein can be combined with any one or more of the other embodiments unless otherwise specified and provided that the combinations are not mutually exclusive.

[0269] Also provided are compounds selected from the Examples disclosed herein.The compounds of the embodiments herein may also refer to derivatives thereof, or the foregoing combinations of the compounds of the embodiments herein.

[0270] Conventional techniques for the preparation / separation of individual enantiomers include chiral synthesis from suitable enantiomerically enriched or optically pure precursors or separation of racemates using, for example, chiral high pressure liquid chromatography (HPLC). Alternatively, the racemate (or racemic precursor) can be reacted with a suitable optically active compound (e.g., alcohol, or an acid or base (such as tartaric acid or 1-phenylethylamine) (when the compound contains an acidic or basic moiety). The resulting diastereomeric mixture can be separated by chromatography and / or fractional crystallization, and one or both of the diastereomers can be converted into corresponding one or more pure enantiomers by means well known to those skilled in the art. The chiral compounds of the embodiments herein (and chiral precursors thereof) can be obtained in enantiomerically enriched form on an asymmetric resin using chromatography (typically HPLC) wherein the mobile phase consists of a hydrocarbon (typically heptane or hexane), containing from 0 to 50% (typically from 2 to 20%) isopropanol and from 0 to 5% alkylamine (typically 0.1% diethylamine). Concentration of the eluent provides an enriched mixture. Stereoisomers can be separated by conventional techniques known to those skilled in the art. See, for example, "Stereochemistry of Organic Compounds" by Ernest L. Eliel (Wiley, New York, 1994).

[0271] Atropisomers are stereoisomers arising from hindered rotation about a single bond, where the steric strain barrier to rotation is high enough to allow separation of the conformers. Oki (Oki, M; Topics in Stereochemistry 1983, 1) defines atropisomers as conformers that interconvert with a half-life of more than 1000 seconds at a given temperature. The scope of the embodiments described and claimed herein encompasses the racemic forms of the compounds as well as individual atropisomers (atropisomers that are "substantially free" of their corresponding atropisomers) and stereoisomerically enriched mixtures (i.e., mixtures of atropisomers).

[0272] Separation of atropisomers may be performed by chiral resolution methods such as selective crystallization. In atropisomer-enantioselective synthesis or atropisomer-selective synthesis, one atropisomer is formed at the expense of another atropisomer. When the isomerization reaction favors one atropisomer over the other atropisomer, atropisomer-selective synthesis may be performed by using a chiral auxiliary such as a Corey-Bakshi-Shibata (CBS) catalyst (an asymmetric catalyst derived from proline) in the total synthesis of knipholone or by methods based on thermodynamic equilibrium.

[0273] The term "atropisomerism" refers to a type of isomerism caused by hindered rotation about a single bond due to steric strain of a substituent. This phenomenon produces stereoisomers that exhibit axial chirality.

[0274] The following schemes illustrate "atropisomers" for specific pyridone-pyridine compounds of the present invention:

[0275]

[0276] The bond between the B ring and the C ring of the title compound is hindered and does not allow easy rotation. The steric strain barrier of rotation is high enough to separate single conformational isomers. The compounds of the present invention can also exist as atropisomers (i.e., chiral rotamers). The present invention includes racemates, split atropisomers, and mixtures thereof. Atropisomers can be separated via supercritical fluid chromatography using a mobile phase of carbon dioxide and ethanol / methanol.

[0277] Atropisomers are generally stable but can often be in thermal equilibrium. Atropisomers will have the same but opposite optical rotations. Each atropisomer can have different properties when binding to an enzyme or receptor, with one isomer often being more potent than the other. Atropisomers are often used as pharmaceutical agents. Known examples include vancomycin and derivatives.

[0278] As described in Bringmann, G. et.al., Angew. Chem. Int. Ed. 2005, 44, 5384 and references cited therein, the configuration of atropisomers can be described using the nomenclature (M)- and (P)- to describe the relative positions of substituents. The structures are designated as drawn, but it should be understood that either the (P)-isomer or the (M)-isomer may be desired, and the methods described will be useful for interconversion of (P)-stereoisomers or (M)-stereoisomers.

[0279] The term "interconversion" or "conformational interconversion" refers to any change between atropisomers of the present disclosure, including but not limited to equilibration.

[0280] The term "equilibrium" refers to a chemical reaction in which the forward and reverse specific rates cancel each other out. Equilibrium can be dynamic or static. A reaction in equilibrium need not contain equal amounts of reactants and products.

[0281] Suitable pharmaceutically acceptable acid addition salts of the compounds of the embodiments herein may be prepared from inorganic or organic acids. All of these salts may be prepared from the corresponding compounds of the embodiments herein by conventional means, for example, by treating the compounds with an appropriate acid or base.

[0282] Pharmaceutically acceptable acids include inorganic acids (e.g., hydrochloric acid, hydrobromic acid, hydroiodic acid, nitric acid, carbonic acid, sulfuric acid, phosphoric acid and pyrophosphoric acid) and organic acids (e.g., formic acid, acetic acid, trifluoroacetic acid, propionic acid, succinic acid, glycolic acid, enbolic acid (pamoic acid), methanesulfonic acid, ethanesulfonic acid, 2-hydroxyethanesulfonic acid, pantothenic acid, benzenesulfonic acid, toluenesulfonic acid, sulfanilic acid, mesylic acid, cyclohexylaminosulfonic acid, stearic acid, alginic acid, β-hydroxybutyric acid, malonic acid, galactic acid, galacturonic acid, citric acid, fumaric acid, gluconic acid, glutamic acid, lactic acid, maleic acid, malic acid, mandelic acid, mucic acid, ascorbic acid, oxalic acid, pantothenic acid, succinic acid, tartaric acid, benzoic acid, acetic acid, xinafoic acid (1-hydroxy-2-naphthoic acid), naphthalene disulfonic acid (napadisilic acid) acid) (1,5-naphthalenedisulfonic acid, etc.).

[0283] Salts derived from pharmaceutically acceptable inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, zinc, and the like. Salts derived from pharmaceutically acceptable organic bases include salts of primary, secondary and tertiary amines, including alkylamines, arylalkylamines, heterocyclic amines, cyclic amines, naturally occurring amines, and the like, such as arginine, betaine, caffeine, choline, chloroprocaine, diethanolamine, N-methylglucamine, N,N'-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucosamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purine, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.

[0284] Other preferred salts according to embodiments of the present invention are quaternary ammonium compounds, wherein the equivalent of the anion (X-) is related to the positive charge of the N atom. X- can be an anion of various inorganic acids (such as, for example, chloride, bromide, iodide, sulfate, nitrate, phosphate), or an anion of an organic acid (such as, for example, acetate, maleate, fumarate, citrate, oxalate, succinate, tartrate, malate, mandelate, trifluoroacetate, methanesulfonate and p-toluenesulfonate). X- is preferably an anion selected from chloride, bromide, iodide, sulfate, nitrate, acetate, maleate, oxalate, succinate or trifluoroacetate. More preferably, X- is chloride, bromide, trifluoroacetate or methanesulfonate.

[0285] As used herein, N-oxides are formed from tertiary basic amines or imines present in the molecule using a convenient oxidizing agent.

[0286] The compounds of the embodiments herein may exist in unsolvated and solvated forms. The term solvate is used herein to describe a molecular complex including a compound of the embodiments herein and a certain amount of one or more pharmaceutically acceptable solvent molecules. When the solvent is water, the term hydrate is used. Examples of solvate forms include, but are not limited to, compounds of the embodiments herein associated with water, acetone, dichloromethane, 2-propanol, ethanol, methanol, dimethyl sulfoxide (DMSO), ethyl acetate, acetic acid, ethanolamine, or a mixture thereof. It is particularly contemplated that, in embodiments herein, a solvent molecule may be associated with a molecule of a compound of the embodiments herein, such as a hydrate.

[0287] In addition, it is specifically contemplated that, in embodiments herein, more than one solvent molecule may be associated with one molecule of a compound of embodiments herein, such as a dihydrate. In addition, it is specifically contemplated that, in embodiments herein, less than one solvent molecule may be associated with one molecule of a compound of embodiments herein, such as a hemihydrate. In addition, solvates of embodiments herein are contemplated as solvates of compounds of embodiments herein that retain the biological effectiveness of the non-solvated form of the compound.

[0288] The embodiments herein also include isotopically labeled compounds of the embodiments herein, wherein one or more atoms are replaced by atoms having the same atomic number but an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes suitable for inclusion in the compounds of the embodiments herein include isotopes of hydrogen (e.g. 2 H and 3 H), carbon isotopes (such as 11 C. 13 C and 14 C), isotopes of chlorine (such as 31Cl), fluorine isotopes (such as 18 F), isotopes of iodine (such as 123 I and 125 I), nitrogen isotopes (such as 13 N and 15 N), oxygen isotopes (such as 15 O. 17 O and 18 O), phosphorus isotopes (such as 32 P), and sulfur isotopes (such as 35 S). Certain isotopically labeled compounds of the embodiments herein (e.g., those incorporating a radioactive isotope) are useful in drug and / or substrate tissue distribution studies. 3 H) and carbon-14 ( 14 C) are easily incorporated and readily available for detection, which is particularly useful for this purpose. 2 H) substitution may offer certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and thus may be preferred in some circumstances. 11 C. 18 F. 15 O and 13 N) substitutions may be useful in positron emission tomography (PET) studies for examining substrate receptor occupancy.

[0289] Isotopically-labeled compounds of the embodiments herein can generally be prepared by conventional techniques known to those skilled in the art or by methods analogous to those described herein, using an appropriate isotopically-labeled reagent in place of the non-labeled reagent otherwise employed.

[0290] Preferred isotopically labeled compounds include deuterated derivatives of the compounds of the embodiments herein. As used herein, the term deuterated derivatives includes compounds of the embodiments herein in which at least one hydrogen atom at a specific position is replaced by deuterium. Deuterium (D or 2 H) is a stable isotope of hydrogen that occurs at a natural abundance of 0.015 mol %.

[0291] A hydrogen-deuterium exchange (deuterium incorporation) is a chemical reaction in which a covalently bonded hydrogen atom is replaced by a deuterium atom. The exchange (incorporation) reaction may be complete or partial.

[0292] Typically, a deuterated derivative of a compound of the embodiments herein has an isotopic enrichment factor (the ratio between the isotopic abundance and the natural abundance of that isotope, i.e., the percentage of deuterium incorporated in place of hydrogen at a given position in a molecule) of at least 3500 (52.5% deuterium incorporation) for each deuterium present at a site on the compound designated as a potential deuteration site.

[0293] In some embodiments, the isotopic enrichment factor is at least 5000 (75% deuterium). In some embodiments, the isotopic enrichment factor is at least 6333.3 (95% deuterium incorporation). In some embodiments, the isotopic enrichment factor is at least 6633.3 (99.5% deuterium incorporation). It should be understood that the isotopic enrichment factor of each deuterium present at a site designated as a deuterated site is independent of the other deuterated sites.

[0294] The isotopic enrichment factor can be determined using conventional analytical methods known to those of ordinary skill in the art, including mass spectrometry (MS) and nuclear magnetic resonance (NMR).

[0295] Prodrugs of compounds described herein are also within the scope of embodiments herein. Therefore, some derivatives of compounds of embodiments herein (the derivatives themselves may have little pharmacological activity or no pharmacological activity) can be converted into compounds of embodiments herein with desired activity, for example, by hydrolytic cleavage when applied to or on the body. Such derivatives are referred to as 'prodrugs'. Further information on the application of prodrugs can be found in Pro-drugs as Novel Delivery Systems, Vol. 14, ACS Symposium Series (T. Higuchi and W. Stella) and Bioreversible Carriers in Drug Design, Pergamon Press, 1987 (ed. EB Roche, American Pharmaceutical Association).

[0296] Prodrugs according to the embodiments herein can be prepared, for example, by replacing appropriate functional groups present in the compounds of the embodiments herein with certain moieties known to those skilled in the art as 'pro-moieties', as described, for example, in Design of Prodrugs by H. Bundgaard (Elsevier, 1985).

[0297] The compounds disclosed herein may exist as therapeutically acceptable salts. The present invention includes compounds listed above in the form of salts (including acid addition salts). Suitable salts include salts formed with organic or inorganic acids. Such acid addition salts are generally pharmaceutically acceptable. However, salts of non-pharmaceutically acceptable salts may have utility in the preparation and purification of the compounds in question. Base addition salts may also be formed and are pharmaceutically acceptable. For a more complete discussion of the preparation and selection of salts, refer to Pharmaceutical Salts: Properties, Selection, and Use (Stahl, P. Heinrich. Wiley-VCHA, Zurich, Switzerland, 2002).

[0298] Base addition salts can be prepared during the final separation and purification of the compound by reacting the carboxyl group with a suitable base (such as hydroxide, carbonate, or bicarbonate of a metal cation) or with ammonia or an organic primary amine, secondary amine, or tertiary amine. The cation of the therapeutically acceptable salt includes lithium, sodium, potassium, calcium, magnesium, and aluminum, and nontoxic quaternary amine cations (such as ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, diethylamine, ethylamine, tributylamine, pyridine, N, N-dimethylaniline, N-methylpiperidine, N-methylmorpholine, dicyclohexylamine, procaine, dibenzylamine, N, N-dibenzylphenethylamine, 1-diphenylhydroxymethylamine, and N, N'-dibenzylethylenediamine). Other representative organic amines useful for the formation of base addition salts include ethylenediamine, ethanolamine, diethanolamine, piperidine, and piperazine.

[0299] The present disclosure provides compounds having the structure of Formula (P)-Ia:

[0300]

[0301] in:

[0302] X is CH or N;

[0303] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0304] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0305] R 3 Selected from the group consisting of:

[0306]

[0307] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0308] R 5 Selected from H or C1-C3 alkyl;

[0309] m is 1 or 2;

[0310] n is 0 or 1;

[0311] p is 1;

[0312] q is 0 or 1; or

[0313] Derivatives of compounds having the structure of Formula (P)-Ia.

[0314] In certain embodiments, R 3 Selected from the group consisting of 3,5-difluoropyridin-2-yl, 3-fluoropyridin-2-yl, 5-fluoro-3-methylpyridin-2-yl, 6-fluoropyridin-2-yl, 6-fluoro-4-methylpyridin-2-yl, 3-fluoro-5-methylpyridin-2-yl and 5-fluoropyridin-2-yl.

[0315] Some embodiments are directed to compounds having the structure of Formula (P)-IIa:

[0316]

[0317] in:

[0318] X is CH or N;

[0319] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0320] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0321] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0322] R 5 is H or C1-C3 alkyl;

[0323] m is 1 or 2;

[0324] n is 0 or 1; or

[0325] Derivatives of compounds having the structure of Formula (P)-IIa.

[0326] Non-limiting examples of compounds of formula (P)-IIa include the following compounds or derivatives thereof:

[0327]

[0328]

[0329]

[0330]

[0331]

[0332]

[0333]

[0334]

[0335]

[0336]

[0337]

[0338]

[0339] Some embodiments are directed to compounds having the structure of Formula (P)-IIIa:

[0340]

[0341] in:

[0342] X is CH or N;

[0343] R 1 is chlorine or bromine;

[0344] R 2 is H or methyl; and

[0345] Derivatives of compounds having the structure of Formula (P)-IIIa.

[0346] Non-limiting examples of compounds of formula (P)-IIIa include the following compounds or derivatives thereof:

[0347]

[0348]

[0349]

[0350] Some embodiments are directed to compounds having the structure of Formula (P)-IVa:

[0351]

[0352] in:

[0353] X is CH or N;

[0354] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0355] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0356] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0357] R 5 is H or C1-C3 alkyl;

[0358] m is 1 or 2;

[0359] n is 0 or 1; or

[0360] Derivatives of compounds having the structure of Formula (P)-IVa.

[0361] Some embodiments are directed to compounds having the structure of Formula (P)-Va:

[0362]

[0363] in:

[0364] X is CH or N;

[0365] R 1 is chlorine or bromine;

[0366] R 2 is H or methyl; or

[0367] Derivatives of compounds having the structure of Formula (P)-Va.

[0368] Non-limiting examples of compounds of formula (P)-Va include the following compounds or derivatives thereof:

[0369]

[0370]

[0371]

[0372] Some embodiments are directed to compounds having the structure of Formula (P)-VIa:

[0373]

[0374] in:

[0375] X is CH or N;

[0376] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0377] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0378] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0379] R 5 is H or C1-C3 alkyl;

[0380] p is 1;

[0381] q is 0 or 1; or

[0382] A derivative of a compound having a structure of Formula (P)-VIa.

[0383] Some embodiments are directed to compounds having the structure of Formula (P)-VIIa:

[0384]

[0385] in:

[0386] X is CH or N;

[0387] R 1 is chlorine or bromine;

[0388] R 2 is -H or methyl; or

[0389] Derivatives of the compound having the structure of Formula (P)-VIIa.

[0390] Non-limiting examples of compounds of formula (P)-VIIa include the following compounds or derivatives thereof:

[0391]

[0392]

[0393]

[0394] In certain embodiments, each of the compounds of Formula (P)-Ia, Formula (P)-IIa, Formula (P)-IIIa, Formula (P)-Iva, Formula (P)-Va, Formula (P)-VIa, and Formula (P)-VIIa is substantially free of its corresponding M isomer.

[0395] In some embodiments, the chemical purity of any of the compounds of Formula (P)-Ia, Formula (P)-IIa, Formula (P)-IIIa, Formula (P)-IVa, Formula (P)-Va, Formula (P)-VIa, or Formula (P)-VIIa is about 95% or more, about 96% or more, about 97% or more, about 98% or more, about 98.5% or more, about 99% or more, about 91% or more, about 93% or more, about 97% or more, about 98% or more, about 99% or more, about 91% or more, about 91% or more, about 93 ... About 99.5% or more, about 99.8% or more, about 99.9% or more, about 99.91% or more, about 99.92% or more, about 99.93% or more, about 99.94% or more, about 99.95% or more, about 99.96% or more, about 99.97% or more, about 99.98% or more, or about 99.99% or more.

[0396] The present disclosure includes embodiments directed to a crystalline form of Compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof:

[0397]

[0398] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0399] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0400] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0401] Compound 49a.

[0402] In some embodiments, compound 49a is the free base.

[0403] In some embodiments, Compound 49a is a pharmaceutically acceptable salt.

[0404] In some embodiments, the pharmaceutically acceptable salt is the HCl salt.

[0405] In some embodiments, the crystalline form of Compound 49a is Form A.

[0406] In some embodiments, the crystalline Form A of Compound 49a is anhydrous.

[0407] In some embodiments, the crystalline Form A of compound 49a is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78. In some embodiments, Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78 and about 15.51. In some embodiments, the crystalline Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78, about 15.51, about 19.6, and about 25.92. In some embodiments, the crystalline Form A is characterized by having an XRPD pattern with peaks represented by angle 2θ (± 0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.48. In some embodiments, the crystalline Form A of compound 49a is characterized by Figure 4 XRPD pattern of .

[0408] In one embodiment, provided herein is a crystalline Form A of Compound 49a having substantially the same Figure 5 The TGA thermal curve corresponding to the representative TGA thermogram depicted in . In some embodiments, negligible weight loss is observed. For crystalline Form A of compound 49a, weight loss (0.7%) was observed between 25-256° C. by TGA.

[0409] In one embodiment, provided herein is a crystalline Form A of Compound 49a having substantially the same Figure 5 In certain embodiments, the crystalline Form A of compound 49a is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C and a final sharp endothermic melting event at about 254°C.

[0410] In some embodiments, the crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, contains no more than about 0.01 mol%, about 0.02 mol%, about 0.03 mol%, about 0.04 mol%, about 0.05 mol%, about 0.06 mol%, about 0.07 mol%, about 0.08 mol%, about 0.09 mol%, about 0.1 mol%, about 0.15 mol%, about 0.2 mol%, about 0.25 mol%, about 0.3 mol%, about 0.35 mol%, about 0.4 mol%, about 0.45 mol%, about 0.5 mol%, about 0.55 mol%, about 0.6 mol%, about 0.65 mol%. In some embodiments, the crystalline form of compound 49a, or its pharmaceutically acceptable salt or its free alkali contain no more than about 0.25mol% of the corresponding M isomer of compound 49a, or its pharmaceutically acceptable salt or its free alkali. In some embodiments, the crystalline form of Compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, is substantially free of the corresponding M isomer of Compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof.

[0411] In some embodiments, compound 49a has a chemical purity of about 95% or more, about 96% or more, about 97% or more, about 98% or more, about 98.5% or more, about 99% or more, about 99.5% or more, or about 99.8% or more. In some embodiments, compound 49a is substantially pure.

[0412] In some embodiments, the crystalline Form A of compound 49a contains no more than about 0.01 mol%, about 0.02 mol%, about 0.03 mol%, about 0.04 mol%, about 0.05 mol%, about 0.06 mol%, about 0.07 mol%, about 0.08 mol%, about 0.09 mol%, about 0.1 mol%, about 0.15 mol%, about 0.2 mol%, about 0.25 mol%, about 0.3 mol%, about 0.35 mol%, about 0.4 mol%, about 0.45 mol%, about 0.5 mol%, about 0.55 mol%, about 0.6 mol%, about 0.65 mol%. In some embodiments, the crystalline form A of compound 49a is substantially free of other solid forms.

[0413] Pharmaceutical composition

[0414] Some embodiments herein are directed to pharmaceutical compositions, which include a compound of the embodiments herein or a crystalline form of a compound and a pharmaceutically acceptable excipient. In some embodiments, each of the compositions includes a compound selected from formula (P)-Ia, formula (P)-IIa, formula (P)-IIIa, formula (P)-IVa, formula (P)-Va, formula (P)-VIa, and formula (P)-VIIa. In some embodiments, the pharmaceutical composition is substantially free of any corresponding M isomer.

[0415] The present disclosure includes embodiments directed to pharmaceutical compositions comprising a crystalline form of Compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof:

[0416]

[0417] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0418] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0419] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0420] Compound 49a,

[0421] and pharmaceutically acceptable excipients.

[0422] The present disclosure also includes a pharmaceutical composition comprising compound 49a, or a pharmaceutically acceptable salt thereof or a free base thereof, and compound 49b, or a pharmaceutically acceptable salt thereof or a free base thereof:

[0423]

[0424] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0425] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0426] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0427] Compound 49a,

[0428]

[0429] (M)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0430] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0431] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0432] Compound 49b,

[0433] wherein the molar ratio of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, to compound 49b, or a pharmaceutically acceptable salt thereof, or a free base thereof, is about 4:1;

[0434] and pharmaceutically acceptable excipients.

[0435] In some embodiments, the pharmaceutical compositions disclosed herein include a pharmaceutically acceptable salt of Compound 49a, wherein the pharmaceutically acceptable salt is an HCl salt.

[0436] In some embodiments, the pharmaceutical compositions disclosed herein include the free base of compound 49a. In some embodiments, the pharmaceutical compositions disclosed herein include the free base of compound 49b.

[0437] In some embodiments, the pharmaceutical compositions disclosed herein include a crystalline form of Compound 49a. In some embodiments, the crystalline form of Compound 49a is Form A.

[0438] In some embodiments, the pharmaceutical compositions disclosed herein include crystalline Form A of Compound 49a, and the crystalline Form A of Compound 49a is anhydrous.

[0439] In some embodiments, the pharmaceutical compositions disclosed herein include crystalline Form A of compound 49a, which is characterized by having an XRPD pattern with peaks represented by angles 2θ (± 0.2) at about 9.78. In some embodiments of the pharmaceutical compositions disclosed herein, Form A is characterized by having an XRPD pattern with peaks represented by angles 2θ (± 0.2) at about 9.78 and about 15.51. In some embodiments of the pharmaceutical compositions disclosed herein, crystalline Form A is characterized by having an XRPD pattern with peaks represented by angles 2θ (± 0.2) at about 9.78, about 15.51, about 19.6, and about 25.92. In some embodiments of the pharmaceutical compositions disclosed herein, the crystalline Form A is characterized by an XRPD pattern having peaks represented by angles 2θ (±0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.48.

[0440] In some embodiments, the pharmaceutical compositions disclosed herein include crystalline Form A of Compound 49a, wherein the crystalline Form A of Compound 49a is characterized by Figure 4 XRPD pattern of .

[0441] In one embodiment, the crystalline Form A of compound 49a of the pharmaceutical composition has substantially the same Figure 5 The TGA thermal curve corresponding to the representative TGA thermogram depicted in . In some embodiments of the pharmaceutical compositions disclosed herein, for crystalline Form A of compound 49a, negligible weight loss was observed by TGA between 25-256° C., and weight loss (0.7%) was observed.

[0442] In one embodiment, the crystalline Form A of compound 49a of the pharmaceutical composition has substantially the same Figure 5 In certain embodiments, the crystalline Form A of compound 49a is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C and a final sharp endothermic melting event at about 254°C.

[0443] In some embodiments, the pharmaceutical compositions disclosed herein include a crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, and contain no more than about 0.01 mol%, about 0.02 mol%, about 0.03 mol%, about 0.04 mol%, about 0.05 mol%, about 0.06 mol%, about 0.07 mol%, about 0.08 mol%, about 0.09 mol%, about 0.1 mol%, about 0.15 mol%, about 0.2 mol%, about 0.25 mol%, about 0.3 mol%, about 0.35 mol%, about 0.4 mol%, about 0.45 mol%, about 0.5 mol%, about 0.55 mol%, about 0.6 mol%. %, about 0.65 mol%, about 0.7 mol%, about 0.75 mol%, about 0.8 mol%, about 0.85 mol%, about 0.9 mol%, about 0.95 mol%, about 1 mol%, about 2 mol%, about 3 mol%, about 4 mol%, about 5 mol%, about 6 mol%, about 7 mol%, about 8 mol%, about 9 mol%, about 10 mol%, about 11 mol%, about 12 mol%, about 13 mol%, about 14 mol%, about 15 mol%, about 16 mol%, about 17 mol%, about 18 mol%, about 19 mol%, about 20 mol% of the corresponding M isomer of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof. In some embodiments, the pharmaceutical compositions disclosed herein include a crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, and contain no more than about 0.25 mol% of the corresponding M isomer of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof. In some embodiments, the pharmaceutical compositions disclosed herein include a crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, and are substantially free of the corresponding M isomer of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof.

[0444] In some embodiments, the pharmaceutical compositions disclosed herein include Compound 49a having a chemical purity of about 95% or more, about 96% or more, about 97% or more, about 98% or more, about 98.5% or more, about 99% or more, about 99.5% or more, or about 99.8% or more. In some embodiments, the pharmaceutical compositions disclosed herein include Compound 49a in substantially pure form.

[0445] In some embodiments, the pharmaceutical compositions disclosed herein include crystalline Form A of compound 49a, and the crystalline Form A of compound 49a contains no more than about 0.01 mol%, about 0.02 mol%, about 0.03 mol%, about 0.04 mol%, about 0.05 mol%, about 0.06 mol%, about 0.07 mol%, about 0.08 mol%, about 0.09 mol%, about 0.1 mol%, about 0.15 mol%, about 0.2 mol%, about 0.25 mol%, about 0.3 mol%, about 0.35 mol%, about 0.4 mol%, about 0.45 mol%, about 0.5 mol%, about 0.55 mol%, about 0.6 In some embodiments, the pharmaceutical compositions disclosed herein include crystalline Form A of compound 49a that is substantially free of other solid forms.

[0446] In some embodiments, the pharmaceutical compositions disclosed herein include a therapeutically effective amount of a crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof. In some embodiments, the pharmaceutical compositions disclosed herein include a therapeutically effective amount of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, and compound 49b, or a pharmaceutically acceptable salt thereof, or a free base thereof.

[0447] In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is from about 1 mg to about 1000 mg, from about 1 mg to about 900 mg, from about 1 mg to about 800 mg, from about 1 mg to about 700 mg, from about 1 mg to about 600 mg, from about 1 mg to about 500 mg, from about 1 mg to about 400 mg, from about 1 mg to about 300 mg, from about 1 mg to about 200 mg, from about 1 mg to about 100 mg, from about 10 mg to about 1000 mg, from about 50 mg to about 1000 mg, from about 100 mg to about 1000 mg, from about 200 mg to about 1000 mg, From about 300 mg to about 1000 mg, from about 400 mg to about 1000 mg, from about 500 mg to about 1000 mg, from about 10 mg to about 500 mg, from about 50 mg to about 500 mg, from about 100 mg to about 500 mg, from about 5 mg to about 300 mg, from about 10 mg to about 300 mg, from about 50 mg to about 300 mg, from about 100 mg to about 300 mg, from about 10 mg to about 150 mg, from about 50 mg to about 150 mg, from about 60 mg to about 120 mg, from about 50 mg to about 120 mg, or a range between any two of these values. Specific examples include, for example, about 1000 mg, about 900 mg, about 800 mg, about 700 mg, about 750 mg, about 600 mg, about 500 mg, about 400 mg, about 450 mg, about 300 mg, about 250 mg, about 240 mg, about 200 mg, about 175 mg, about 160 mg, about 150 mg, about 125 mg, about 120 mg, about 110 mg, about 100 mg, about 90 mg, about 80 mg, about 70 mg, about 60 mg, about 50 mg, about 40 mg, about 30 mg, about 20 mg, about 10 mg or any numerical value between the ranges disclosed above. In some embodiments of the pharmaceutical composition disclosed herein, the therapeutically effective amount is about 5 mg to about 300 mg. In some embodiments of the pharmaceutical composition disclosed herein, the therapeutically effective amount is about 240 mg. In some embodiments of the pharmaceutical composition disclosed herein, the therapeutically effective amount is about 200 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 160 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 120 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 100 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 80 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 60 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 50 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 40 mg. In some embodiments of the pharmaceutical compositions disclosed herein, the therapeutically effective amount is about 10 mg.

[0448] In some embodiments of the pharmaceutical compositions disclosed herein, the molar ratio of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, to compound 49b, or a pharmaceutically acceptable salt thereof, or a free base thereof, is about 4.3:1, about 4.6:1, about 4.9:1, about 5.25:1, about 5.7:1, about 6.1:1, about 6.7:1, about 7.3:1, about 8.1:1, about 9:1, about 10:1, about 11.5:1, about 13.3:1, about 15.7:1, about 19:1, about 24:1, about 32.3:1, about 49:1, about 91:1, about 110.1:1, about 124:1, about 141.9:1, about 165.7:1, about 199:1, about 249:1, about 332.3:1, about 399:1, about 499:1 and about 999:1.

[0449] In some embodiments, the pharmaceutical compositions disclosed herein are oral pharmaceutical compositions.

[0450] In some embodiments, the oral pharmaceutical composition is in the form of a tablet.

[0451] Although the compounds described herein may be administered in the form of raw materials, it is also possible to present them as pharmaceutical compositions. Therefore, pharmaceutical compositions are provided herein, and pharmaceutical compositions include one or more of certain compounds disclosed herein and one or more pharmaceutically acceptable excipients and optional one or more other therapeutic ingredients. One or more excipients must be "acceptable" in the sense that they are compatible with the other ingredients of the preparation and harmless to the recipient thereof. The appropriate formulation of the pharmaceutical composition depends on the selected route of administration. Any of the known techniques and excipients can be used as suitable and understood in the art. Pharmaceutical compositions disclosed herein can be manufactured in any manner known in the art, for example, by means of conventional mixing processes, dissolution processes, granulation processes, dragee manufacturing processes, grinding processes, emulsification processes, encapsulation processes, entrapping processes or compression processes.

[0452] In some embodiments, pharmaceutical compositions for use in accordance with the embodiments herein may be formulated in conventional manner using one or more physiologically acceptable excipients.

[0453] Although the most suitable approach may depend on, for example, the patient's condition and disorder, the composition includes compositions suitable for oral administration, parenteral (including subcutaneous, intradermal, intramuscular, intravenous, intraarticular and intramedullary) administration, intraperitoneal administration, intrathecal administration, intradural administration, transmucosal administration, transdermal administration, rectal administration, intranasal administration, topical (including, for example, skin, cheek, sublingual and intraocular) administration, intravitreal administration or intravaginal administration. The composition may include compositions suitable for injection by reservoir or administration by implantation. The composition may include compositions suitable for administration by suction (such as, for example, gas, steam or powder). The composition may include compositions suitable for administration in aerosol form, for example, via a nebulizer, humidifier, inhaler and vaporizer. The composition may be conveniently presented in unit dosage form and may be prepared by any method known in the pharmaceutical field. Typically, these methods include steps of combining the compound disclosed herein ("active ingredient") with a carrier constituting one or more auxiliary components. In general, the compositions are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired composition.

[0454] Compositions of the compounds disclosed herein suitable for oral administration can be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient can also be presented as a bolus, electuary or paste.

[0455] Pharmaceutical compositions that can be used orally include tablets, push-fit capsules made of gelatin, and sealed soft capsules made of gelatin and plasticizers (such as glycerol or sorbitol). Tablets can be made by compression or molding, optionally with one or more auxiliary ingredients. Compressed tablets can be prepared by compressing the active ingredient (such as powder or granules) in a free-flowing form (optionally mixed with a binder, an inert diluent, or a lubricant, a surfactant or a dispersant) in a suitable machine. Die-stamped tablets can be made by molding a mixture of powdered compounds moistened with an inert liquid diluent in a suitable machine. Tablets can be optionally coated or scored, and can be formulated to provide a slow release or controlled release of the active ingredient therein. The entire composition for oral administration should be in a dosage suitable for such administration. Push-fit capsules can contain active ingredients mixed with fillers (such as lactose), binders (such as starch), and / or lubricants (such as talc or magnesium stearate), and optional stabilizers. In soft capsules, the active compound can be dissolved in or suspended in a suitable liquid (such as fatty oil, liquid paraffin or liquid polyethylene glycol). In addition, a stabilizing agent can be added. The dragee core is provided with a suitable coating. For this reason, concentrated sugar solution can be used, and concentrated sugar solution can contain gum arabic, talcum, polyvinyl pyrrolidone, carbomer gel, polyethylene glycol, and / or titanium dioxide, lacquer solution and a suitable organic solvent or solvent mixture alternatively. Coloring agent or pigment can be added to tablet or dragee coating to identify or characterize the different combinations of active compound dosage.

[0456] The compound can be formulated for parenteral administration by injection (e.g., by bolus injection or continuous infusion). Compositions for injection can be present in unit dosage form, for example, in ampoules or multidose containers with added preservatives. Pharmaceutical compositions can take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and can contain formulatory agents (e.g., suspending agents, stabilizers and / or dispersants). Compositions can be present in unit dose containers or multidose containers (e.g., sealed ampoules and vials), and can be stored in powder form or stored under freeze drying (lyophilization) conditions that require only the addition of a sterile liquid carrier (e.g., saline or sterile pyrogen-free water) prior to use. Extemporaneous injections and suspensions can be prepared from sterile powders, granules and tablets of the aforementioned types.

[0457] Pharmaceutical compositions for parenteral administration include aqueous and non-aqueous (oily) sterile injections of the active compound (which may contain antioxidants, buffers, bacteriostats, and solutes that make the composition isotonic with the blood of the intended recipient); and aqueous and non-aqueous sterile suspensions (which may contain suspending agents and thickening agents). Suitable lipophilic solvents or vehicles include fatty oils (such as sesame oil), or synthetic fatty acid esters (such as ethyl oleate or triglycerides), or liposomes. Aqueous injection suspensions may contain substances that increase the viscosity of the suspension (such as sodium carboxymethylcellulose, sorbitol, or dextran). Optionally, the suspension may also contain a suitable stabilizer or an agent that increases the solubility of the compound to allow the preparation of a highly concentrated solution.

[0458] In addition to the pharmaceutical compositions described previously, the compound can also be formulated into a depot preparation. Such a long-acting composition can be administered by implantation (e.g., subcutaneously or intramuscularly) or by intramuscular injection. Therefore, for example, the compound can be formulated with a suitable polymeric material or hydrophobic material (e.g., as an emulsion in an acceptable oil) or an ion exchange resin, or be formulated into a slightly soluble derivative (e.g., formulated into a slightly soluble salt).

[0459] For buccal or sublingual administration, the pharmaceutical composition may take the form of tablets, lozenges, pastilles, or gels formulated in conventional manner. Such compositions may include the active ingredient in a flavored basis such as sucrose and acacia or tragacanth.

[0460] The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, eg, containing conventional suppository bases such as cocoa butter, polyethylene glycol, or other glycerides.

[0461] Certain compounds disclosed herein can be administered topically (i.e., by non-systemic administration). This includes applying compounds disclosed herein externally to the epidermis or buccal cavity, and dropping such compounds into the ears, eyes, and nose. In contrast, systemic administration refers to oral administration, intravenous administration, intraperitoneal administration, and intramuscular administration.

[0462] In some embodiments, pharmaceutical compositions suitable for topical administration include liquid or semi-liquid preparations suitable for penetration through the skin to reach the site of inflammation (such as solutions, powders, liquid emulsions, liquid suspensions, semisolids, ointments, pastes, creams, gels, jelly, foams, liniments, lotions, and drops suitable for administration to the eye, ear, or nose). The active ingredient for topical administration may account for, for example, from 0.001% w / w to 10% w / w (by weight) of the composition. In certain embodiments, the active ingredient may account for up to 10% w / w. In other embodiments, it may account for less than 5% w / w. In certain embodiments, the active ingredient may account for from 2% w / w to 5% w / w. In other embodiments, it may account for from 0.1% w / w to 1% w / w of the composition.

[0463] Gels for topical or transdermal administration may generally include a mixture of a volatile solvent, a nonvolatile solvent, and water. In certain embodiments, the volatile solvent component of the buffered solvent system may include lower (C1-C6) alkyl alcohols, lower alkyl glycols, and lower glycol polymers. In other embodiments, the volatile solvent is ethanol. The volatile solvent component is considered to act as a penetration enhancer, and also produces a cooling effect on the skin when evaporated. The nonvolatile solvent portion of the buffered solvent system is selected from lower alkylene glycols and lower glycol polymers. In certain embodiments, propylene glycol is used. The nonvolatile solvent slows down the evaporation of the volatile solvent and reduces the vapor pressure of the buffered solvent system. As with volatile solvents, the amount of this nonvolatile solvent component is determined by the pharmaceutical compound or medicine used. When the nonvolatile solvent in the system is too little, the pharmaceutical compound may crystallize due to the evaporation of the volatile solvent, and excessive amounts may cause a lack of bioavailability due to poor release of the drug from the solvent mixture. The buffer component of the buffered solvent system can be selected from any buffer commonly used in the art; in certain embodiments, water is used. Common proportions of the ingredients are about 20% non-volatile solvent, about 40% volatile solvent, and about 40% water. There are several optional ingredients that can be added to the topical composition. These include, but are not limited to, chelating agents and gelling agents. Suitable gelling agents can include, but are not limited to, semisynthetic cellulose derivatives (such as hydroxypropyl methylcellulose) and synthetic polymers and cosmetic agents.

[0464] Lotions include those suitable for application to the skin or eyes. Eye lotions may include sterile aqueous solutions optionally containing bactericides, and may be prepared by methods similar to those for preparing drops. Lotions or liniments for application to the skin may also include agents (such as alcohol or acetone) that accelerate drying and cool the skin, and / or humectants (such as glycerol) or oils (such as castor oil or peanut oil).

[0465] Cream, ointment or paste is a semisolid pharmaceutical composition of an active ingredient for external use. It can be made by mixing the active ingredient in finely crushed or powdered form (alone or in a solution or suspension in an aqueous or non-aqueous fluid) with an oily or non-oily matrix by means of a suitable machine. The matrix can include hydrocarbons, such as hard paraffin, soft paraffin or liquid paraffin, glycerol, beeswax, metal soaps; mucilage; oils of natural origin, such as almond oil, corn oil, peanut oil, castor oil or olive oil; lanolin or its derivatives or fatty acids (such as stearic acid or oleic acid) and alcohols (such as propylene glycol) or macrogranular gels. The pharmaceutical composition can incorporate any suitable surfactant, such as anionic surfactants, cationic surfactants or nonionic surfactants (such as sorbitan esters or their polyethylene oxide derivatives). It can also include suspending agents (such as natural gums), cellulose derivatives or inorganic materials (such as siliceous silica), and other ingredients (such as lanolin).

[0466] Drops can include aseptic aqueous or oily solutions or suspensions, and can be prepared by dissolving active ingredients in a suitable aqueous solution of bactericide and / or fungicide and / or any other suitable preservative (and comprising surfactant in certain embodiments). Then, the resulting solution can be clarified by filtration, transferred to a suitable container, sealed container afterwards, and sterilized by autoclaving or keeping at 98 ℃-100 ℃ for half an hour. Alternatively, the solution can be sterilized by filtration, and transferred to a container by aseptic technique. Examples of bactericides and fungicides suitable for inclusion in drops are phenylmercuric nitrate or acetate (0.002%), benzalkonium chloride (0.01%) and chlorhexidine acetate (0.01%). Suitable solvents for the preparation of oily solutions include glycerol, diluted alcohol and propylene glycol.

[0467] Pharmaceutical compositions for topical administration in the mouth, for example buccal or sublingual, include lozenges comprising the active ingredient in a flavored base such as sucrose and acacia or tragacanth and pastilles comprising the active ingredient in a base such as gelatin and glycerin or sucrose and acacia.

[0468] For administration by inhalation, the compound can be conveniently delivered from an insufflator, atomizer pressurized packaging or other convenient means of delivering an aerosol spray. The pressurized packaging can include a suitable propellant, such as dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit can be determined by providing a valve to deliver a metered amount. Alternatively, for administration by inhalation or insufflation, the compound according to the present invention can take the form of a dry powder composition (e.g., a powder mixture of the compound and a suitable powder matrix (e.g., lactose or starch)). The powder composition can be present in, for example, a capsule, a cartridge, a gelatin package or a blister package in a unit dosage form, from which the powder can be administered by means of an insufflator or insufflator.

[0469] Preferred unit dosage pharmaceutical compositions are those containing a therapeutically effective amount, or an appropriate fraction, of the active ingredient as recited herein below.

[0470] It should be understood that in addition to the ingredients particularly mentioned above, the pharmaceutical compositions described above may include other agents conventional in the art having regard to the type of pharmaceutical composition in question, for example those suitable for oral administration may include flavoring agents.

[0471] The compound can be administered at a dosage of from 0.1 mg / kg to 500 mg / kg per day. The dosage range for adults is generally from 5 mg / day to 2 g / day. The tablet or other presentation provided in discrete units can conveniently contain one or more compounds in such a dosage or as a multiple effective amount of an equal dose, for example, a unit containing 5 mg to 500 mg (usually about 10 mg to 200 mg).

[0472] The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

[0473] When used as a medicine, the compound can be administered in the form of a pharmaceutical composition. These compositions can be prepared in a manner known to the pharmaceutical field, and can be administered by a variety of routes depending on whether local treatment or systemic treatment and the area to be treated are desired. The administration of the disclosed compound or composition can be oral administration, parenteral (including subcutaneous, intradermal, intramuscular, intravenous, intraarticular and intramedullary) administration, pulmonary (for example, by inhalation or insufflation of powders or aerosols (including by a nebulizer); intratracheal or intranasal) administration, intraperitoneal administration, intracapsular administration, intradural administration, transmucosal administration, transdermal administration, rectal administration, local (including skin, cheek, sublingual and intraocular) administration or intravaginal administration. Parenteral administration includes intravenous administration, intraarterial administration, subcutaneous administration, intraperitoneal administration, intramuscular administration or injection administration or infusion administration; or intracranial (for example, intrathecal or intraventricular) administration. Parenteral administration can be in the form of a single dose, or can be performed, for example, by a continuous infusion pump. The pharmaceutical composition for topical application can include foams, transdermal patches, ointments, lotions, creams, gels, solutions, liquid emulsions, liquid suspensions, semisolids, pastes, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous powders or oily bases, thickeners, etc. may be necessary or desirable. Coated condoms, gloves, etc. may also be useful. In some embodiments, the compound may be contained in such a pharmaceutical composition together with a pharmaceutically acceptable diluent, filler, disintegrant, binder, lubricant, surfactant, hydrophobic solvent, water-soluble solvent, emulsifier, buffer, wetting agent, humectant, solubilizer, preservative, etc. Technicians may refer to various pharmaceutical references as guidance. For example, one may refer to Modern Pharmaceutics, 5th Edition, Banker & Rhodes, CRC Press (2009); and Goodman & Gilman's The Pharmaceutical Basis of Therapeutics, 13th Edition, McGraw Hill, New York (2018).

[0474] In some embodiments, the method of treating a p38 MAP kinase-mediated disease comprises administering a pharmaceutical composition of an embodiment disclosed herein. In some embodiments, the compound is in a therapeutically effective amount. In some embodiments, the therapeutically effective amount is an amount disclosed herein.

[0475] In some embodiments, the method of making a pharmaceutical composition includes mixing the active ingredient with an excipient, diluting the active ingredient with an excipient, or enclosing the active ingredient in a carrier, such as in the form of a capsule, sachet, paper, or other container. When the excipient acts as a diluent, it can be a solid material, a semisolid material, or a liquid material, which acts as a vehicle, carrier, or medium for the active ingredient. Thus, the pharmaceutical composition can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols (as solids or in liquid media), ointments (containing, for example, up to 10% by weight of the active compound), soft and hard gelatin capsules, suppositories, sterile injectable solutions, and sterile packaged powders.

[0476] Some examples of suitable excipients include the following: lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum arabic, calcium phosphate, alginate, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup and methylcellulose containing a deep eutectic solvent, a deep eutectic ionic liquid, or an ionic liquid. The pharmaceutical composition may additionally include: lubricants such as talc, magnesium stearate and mineral oil; wetting agents; emulsifiers and suspending agents; preservatives such as methyl hydroxybenzoate and propyl hydroxybenzoate; sweeteners; and flavoring agents. By using procedures known in the art, the pharmaceutical composition can be formulated to provide rapid release, sustained release or delayed release of the active ingredient after administration to the patient.

[0477] The pharmaceutical compositions can be formulated in unit dosage form. The term "unit dosage form" refers to physically discrete units suitable as unit dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.

[0478] The active compound can be effective over a wide dosage range and can generally be administered in a therapeutically effective amount. However, it will be understood that the amount of compound actually administered will generally be determined by a physician based on relevant circumstances (including the condition to be treated, the route of administration selected, the compound actually administered, the age, weight and response of the individual patient, the severity of the patient's symptoms, etc.).

[0479] In some embodiments, a pharmaceutical composition may include about 0.01% to about 50% of one or more compounds disclosed herein. In some embodiments, the content of one or more compounds is about 0.01% to about 50%, about 0.01% to about 45%, about 0.01% to about 40%, about 0.01% to about 30%, about 0.01% to about 20%, about 0.01% to about 10%, about 0.01% to about 5%, about 0.05% to about 50%, about 0.05% to about 45%, about 0.05 ...10%, about 0.01% to about 10%, about 0.0 .05% to about 30%, about 0.05% to about 20%, about 0.05% to about 10%, about 0.1% to about 50%, about 0.1% to about 45%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 5%, about 0.5% to about 50%, about 0.5% to about 45%, about 0.5% to about 40%, From about 0.5% to about 30%, from about 0.5% to about 20%, from about 0.5% to about 10%, from about 0.5% to about 5%, from about 1% to about 50%, from about 1% to about 45%, from about 1% to about 40%, from about 1% to about 35%, from about 1% to about 30%, from about 1% to about 25%, from about 1% to about 20%, from about 1% to about 15%, from about 1% to about 10%, from about 1% to about 5%, from about 5% to about 45%, from about 5 % to about 40%, about 5% to about 35%, about 5% to about 30%, about 5% to about 25%, about 5% to about 20%, about 5% to about 15%, about 5% to about 10%, about 10% to about 45%, about 10% to about 40%, about 10% to about 35%, about 10% to about 30%, about 10% to about 25%, about 10% to about 20%, about 10% to about 15%, or a value within one of these ranges. Specific examples can include about 0.01%, about 0.05%, about 0.1%, about 0.25%, about 0.5%, about 0.75%, about 1%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 60%, about 70%, about 80%, about 90% or the range between any two of these values. The aforementioned all represent the weight percentage of the pharmaceutical composition. In some embodiments, the pharmaceutical composition is suitable for topical application. In some embodiments, the pharmaceutical composition is suitable for oral administration, parenteral (including subcutaneous, intradermal, intramuscular, intravenous, intraarticular and intramedullary) administration, intraperitoneal administration, intrathecal administration, intradural administration, transmucosal administration, transdermal administration, rectal administration, intranasal administration, local (including, for example, skin, buccal, sublingual and intraocular) administration, intravitreal administration, or intravaginal administration.

[0480] In some embodiments, the therapeutically effective amount can vary according to, for example, the specific application for treatment, the mode of administration of the compound, the health and condition of the patient, and the judgment of the prescribing physician. The ratio or concentration of the compound in the pharmaceutical composition can vary according to many factors (including dosage, chemical properties (e.g., hydrophobicity), and route of administration). For example, the compound can be provided in a physiological buffered aqueous solution containing about 0.1% w / v to about 10% w / v of the compound for parenteral administration. Some typical dosage ranges of the compound are from about 1 μg / kg body weight per day to about 1g / kg body weight per day. In some embodiments, the dosage range is from about 0.01 mg / kg body weight per day to about 100 mg / kg body weight per day. In some embodiments, the dosage range is from about 0.1 mg / kg body weight per day to about 10 mg / kg body weight per day. The dosage may depend on variables such as the type and degree of progression of the disease or disorder, the overall health of a particular patient, the relative biological efficacy of the selected compound, the drug composition of the excipient, and its route of administration. The effective dose can be inferred from a dose response curve derived from an in vitro or animal model test system.

[0481] The amount of compound or composition administered to a patient will vary depending on what is being administered, the purpose of the administration (e.g., prevention or treatment), the state of the patient, the mode of administration, etc. In therapeutic applications, the composition can be administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications.

[0482] In order to prepare solid compositions (such as tablets), the main active ingredient can be mixed with a pharmaceutical excipient to form a solid preformulation composition containing a uniform mixture of the compounds of the present invention. When these preformulation compositions are referred to as uniform, the active ingredient is usually evenly dispersed throughout the pharmaceutical composition so that the pharmaceutical composition can be easily and evenly divided into therapeutically effective unit dosage forms (such as tablets, pills and capsules). Then, the solid preformulation is subdivided into the above-mentioned type of unit dosage forms containing, for example, from about 0.1 mg to about 1000 mg of the active ingredient.

[0483] Tablets or pills of the present invention can be coated or otherwise compounded to provide a dosage form that gives the advantage of prolonged action. For example, tablets or pills can include an inner dosage component and an outer dosage component, the latter being the form of a wrapper on the former. These two components can be separated by an enteric layer, which is used to resist disintegration in the stomach, and allows the inner component to enter the duodenum intact or to be delayed for release. Various materials can be used for such enteric layers or coatings, and such materials comprise a mixture of many polymeric acids and polymeric acids and materials (such as shellac, cetyl alcohol and cellulose acetate).

[0484] Liquid forms in which the compounds and compositions of the invention may be incorporated for oral administration or by injection include aqueous solutions, appropriately flavored syrups, aqueous or oily suspensions, and flavored emulsions with edible oils (such as cottonseed oil, sesame oil, coconut oil, or peanut oil), as well as elixirs and similar pharmaceutical vehicles.

[0485] Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable aqueous or organic solvents, or mixtures thereof, and powders. Liquid compositions or solid compositions may contain suitable pharmaceutically acceptable excipients as described above. In some embodiments, the pharmaceutical composition is administered by oral or nasal respiratory routes to produce local or systemic effects. The composition can be atomized by using an inert gas. The atomized solution can be breathed directly from the atomizing device, or the atomizing device can be connected to a face mask tent or an intermittent positive pressure breathing machine. Solution compositions, suspension compositions, or powder compositions can be administered orally or nasally from a device that delivers the composition in an appropriate manner.

[0486] In some embodiments, the pharmaceutical composition used to the patient can be in the form of the above-mentioned pharmaceutical composition. In some embodiments, these compositions can be sterilized by conventional sterilization techniques, or can be aseptically filtered. Aqueous solutions can be packaged to be used as is or lyophilized (lyophilized preparations are combined with sterile aqueous carriers before administration). In some embodiments, the pH of the compound preparation is about 3 to about 11, about 5 to about 9, about 5.5 to about 6.5, or about 5.5 to about 7.5. It will be understood that the use of some of the aforementioned excipients, carriers, or stabilizers will cause the formation of drug salts.

[0487] The composition may further comprise one or more additional agents as described herein, such as a chemotherapeutic agent, a steroid, an anti-inflammatory compound, or an immunosuppressant.

[0488] How to use

[0489] The present invention relates to a method for modulating p38 MAP kinase-mediated function in a subject, the method comprising administering a therapeutically effective amount of a compound disclosed herein. In certain embodiments, each of the compounds of Formula (P)-Ia, Formula (P)-IIa, Formula (P)-IIIa, Formula (P)-IVa, Formula (P)-Va, Formula (P)-VIa, and Formula (P)-VIIa disclosed herein, or a pharmaceutical composition comprising the same, is substantially free of its corresponding M isomer.

[0490] In some embodiments, the method of modulating p38 MAP kinase-mediated function comprises administering a therapeutically effective amount of a crystalline form of compound 49a, or a pharmaceutically acceptable salt thereof or a free base thereof, or a pharmaceutical composition comprising the same. In some embodiments, the compound 49a administered according to these methods is substantially free of its corresponding M isomer. In some embodiments, the crystalline form of compound 49a is Form A.

[0491] The present invention also relates to a method of inhibiting at least one p38 MAP kinase function, the method comprising the step of contacting the p38 MAP kinase with a compound described herein. Cell phenotype, cell proliferation, activity of the p38 MAP kinase, changes in biochemical output produced by active p38 MAP kinase, expression of the p38 MAP kinase, or binding of the p38 MAP kinase to a natural binding partner can be monitored to determine the level of p38 MAP kinase regulation achieved with the compounds described herein. Such methods can be treatment modalities for a disease, biological assays, cell assays, biochemical assays, and the like.

[0492] Also provided herein is a method for treating a p38 MAP kinase-mediated disease, comprising administering a therapeutically effective amount of a compound disclosed herein or a combination thereof to a patient in need thereof. In certain embodiments, a therapeutically effective amount of a compound disclosed herein or a combination thereof may be in the form of a pharmaceutical composition. In an embodiment, the pharmaceutical composition may include a pharmaceutically acceptable excipient.

[0493] In embodiments, the diseases or disorders associated with p38MAP kinase treated by the compounds of the invention include autoimmune disorders, chronic inflammatory disorders, acute inflammatory disorders, autoinflammatory disorders, fibrotic disorders, metabolic disorders, neoplasia, or cardiovascular disorders or cerebrovascular disorders. Thus, in some embodiments, the invention provides methods for treating a p38MAP kinase-mediated disease or disorder in a patient in need thereof, wherein the method comprises administering to the patient a therapeutically effective amount of a provided compound or composition thereof. Such p38MAP kinase-mediated diseases or disorders include, but are not limited to, those described herein.

[0494] In any embodiment, the pharmaceutical compositions disclosed herein are useful for treating chronic or acute inflammatory or autoimmune gastrointestinal disorders, inflammatory or autoimmune skin disorders, neuroinflammatory disorders, inflammatory heart diseases, inflammatory lung diseases, inflammatory myopathies, inflammatory bone disorders or diseases, periodic fever syndromes, and pain or pruritus associated with any of the above diseases.

[0495] In any embodiment, the pharmaceutical compositions disclosed herein may also be used to treat scarring / fibrotic diseases or disorders as well as various types of cancers and hyperproliferative disorders.

[0496] In any embodiment, the pharmaceutical compositions disclosed herein are suitable for treating inflammatory arthritis (such as rheumatoid arthritis (RA), spondyloarthritis (such as ankylosing spondylitis), psoriatic arthritis, reactive arthritis and Reiter's syndrome, juvenile rheumatoid arthritis (JIA), systemic onset juvenile rheumatoid arthritis, idiopathic arthritis (JIA) (including systemic (SJIA)) and gout; cryopyrin-associated autoinflammatory syndrome (CAPS) (including Muckle-Wells syndrome (MWS)), neonatal The following are some of the most common diseases: cold-onset multisystem inflammatory disease (NOMID) and familial cold autoinflammatory syndrome (FCAS)); chronic obstructive pulmonary disease (COPD) (including emphysema, chronic bronchitis and asthma (allergic and non-allergic), hidradenitis suppurativa (HS); psoriasis, such as plaque psoriasis; colitis due to inflammatory bowel disease (IBD) (such as Crohn's disease) or ulcerative colitis, and IBD-related arthritis; pericarditis, including acute pericarditis, recurrent pericarditis and chronic pericarditis; lung inflammation or fibrosis, including idiopathic pulmonary fibrosis; metastatic breast cancer and pancreatic cancer.

[0497] In any embodiment, the pharmaceutical compositions disclosed herein are useful for treating familial Mediterranean fever (FMF); tumor necrosis factor receptor-associated periodic syndrome (TRAPS); adult-onset Still's disease; pyoderma gangrenosum; bone resorption disorders (such as disorders associated with cancer (e.g., breast cancer)); metastatic melanoma; Castleman's disease; and chronic atypical neutrophilic and lipodystrophy (CANDLE).

[0498] In any embodiment, the condition treated according to the methods described herein is pruritus, which may be associated with any other condition, such as pruritus associated with hidradenitis suppurativa, pruritus associated with inflammation, pruritus associated with rheumatoid arthritis, pruritus associated with psoriasis, and pruritus associated with TH17-related inflammation.

[0499] In any embodiment, the pharmaceutical compositions disclosed herein are suitable for treating: Lyme disease; cytokine release syndrome (CRS); adult respiratory distress syndrome (ARDS); chronic or acute bronchitis; epidermolysis bullosa (EB); bullous pemphigoid; juvenile dermatomyositis; inflammatory vitiligo (including marginal); pemphigus vulgaris; enterocolitis; polymyositis; myositis, bone cancer; lung cancer; inflammatory bone disorders (such as chronic relapsing multiple osteomyelitis (CRMO), synovitis, acne, impetigo, osteophyte and osteitis (SAPHO) syndrome, Magid syndrome, interleukin-1 receptor antagonist (DIRA) deficiency and familial macrosomia jaw disorders; bone resorption (e.g., associated with autoimmune diseases); neuroinflammatory diseases (e.g., Alzheimer's disease (AD), Parkinson's disease (PD), multiple sclerosis (MS), acute disseminated encephalomyelitis (ADEM), acute optic neuritis (AON), transverse myelitis, and neuromyelitis optica (NMO); Behcet's disease; endotoxin shock (e.g., toxic shock syndrome (TSS) and other systemic gram-negative bacterial infections); enthesitis; polyarteritis nodosa (PAN); chronic pain; polymyalgia rheumatica; chronic allograft rejection; Sjögren's syndrome; and Schnitzler syndrome (SchS).

[0500] In any embodiment, the p38 MAP kinase mediated disease or disorder is selected from the group consisting of skin disorders, pruritus, hair loss disorders, cancer, neoplasms, Alzheimer's disease, inflammatory conditions, connective tissue diseases, and autoimmune conditions.

[0501] In any embodiment, the p38 MAP kinase mediated disease or disorder is a neoplasm, a malignancy, a myeloproliferative disorder, a hematopoietic neoplasm, a spinal cord neoplasm, a lymphoid neoplasm (including myelofibrosis, primary myelofibrosis, polycythemia vera, essential thrombocythemia, acute leukemia and chronic leukemia, lymphoma, cutaneous lymphoma (including mycosis fungoides), other myeloid malignancies and myelodysplastic syndrome).

[0502] In some embodiments, the methods described herein are used to treat patients with disorders caused by cytokines, enzymes and / or inflammatory mediators produced, stability, secretion, and post-translational processing. In some embodiments, the methods described herein are used to treat patients with cytokine release syndrome, which is a systemic inflammatory response triggered by multiple factors (including infection (e.g., viral infection) and certain drugs (CAR T cell therapy)). Examples of cytokines that may be unregulated in the above-mentioned disorders include interleukin 1, interleukin 2, interleukin 6, interleukin 8, interleukin 10, interleukin 12, interleukin 17, interleukin 22 and interleukin 23, and tumor necrosis factor α and interferon α, interferon β and interferon γ. Examples of inflammatory mediators that may be unregulated include nitric oxide, prostaglandins and leukotrienes. The example of enzyme includes cyclooxygenase, nitric oxide synthase and matrix metalloproteinase.

[0503] Also provided herein are compounds disclosed herein for use as medicaments.

[0504] Also provided herein are compounds disclosed herein for use as medicaments for the treatment of p38 MAP kinase mediated diseases.

[0505] Also provided are compounds disclosed herein for use as medicaments.

[0506] Also provided are the uses of the compounds disclosed herein as medicaments for treating p38 MAP kinase mediated diseases.

[0507] Also provided are compounds disclosed herein for use in the manufacture of a medicament for treating a p38 MAP kinase-mediated disease.

[0508] Also provided are uses of the compounds disclosed herein for treating p38 MAP kinase-mediated diseases.

[0509] Also provided herein are methods of inhibiting p38 MAP kinase, the methods comprising contacting the p38 MAP kinase with a compound disclosed herein.

[0510] The compounds and compositions disclosed herein can be administered in various ways, for example, orally, parenterally (including subcutaneous, intradermal, intramuscular, intravenous, intraarticular, and intramedullary), intraperitoneally, intrathecally, intradurally, transmucosally, transdermally, rectly, intranasally, topically (including, for example, skin, cheek, sublingual, and intraocular), intravitreal, or intravaginally. The specific dosage level for any particular patient will depend on a variety of factors, including the activity of the particular compound employed, age, body weight, general health, sex, diet, administration time, administration route, excretion rate, drug combination, the exact disorder being treated, and the severity of the indication or condition being treated. In addition, the route of administration can vary depending on the condition and its severity.

[0511] In addition to being useful for human treatment, certain compounds and compositions disclosed herein may also be useful for veterinary treatment of companion animals, exotic animals, and farm animals, including mammals, rodents, etc. More preferred animals include horses, dogs, and cats.

[0512] Combination therapy

[0513] The compounds and pharmaceutical compositions of the present disclosure can be used to prevent or treat p38 MAP kinase-mediated diseases by sequential administration or co-administration with additional pharmaceutical agents.

[0514] The compounds of the present invention can be used alone or in combination with other pharmaceutically active compounds to treat conditions (such as those previously described above). One or more compounds of the present invention and other one or more pharmaceutically active compounds can be administered simultaneously (in the same dosage form or in separate dosage forms) or sequentially. Therefore, in one embodiment, the present invention includes methods of treating conditions by administering to a subject a therapeutically effective amount of one or more compounds of the present invention and one or more additional pharmaceutically active compounds.

[0515] In some cases, it may be appropriate to administer at least one of the compounds described herein in combination with another pharmaceutical agent. By way of example only, if one of the side effects experienced by a patient when receiving one of the compounds herein is hypertension, it may be appropriate to administer an antihypertensive agent in combination with an initial pharmaceutical agent. Alternatively, by way of example only, the therapeutic effect of one of the compounds described herein may be enhanced by the administration of an adjuvant (i.e., the adjuvant itself may only have minimal therapeutic benefit, but when combined with another pharmaceutical agent, the overall therapeutic benefit to the patient is enhanced). Alternatively, by way of example only, the benefit experienced by the patient may be increased by administering one of the compounds described herein together with another pharmaceutical agent (which also includes a treatment regimen) that also has a therapeutic benefit. By way of example only, in a treatment for diabetes involving the administration of one of the compounds described herein, an increased therapeutic benefit may also be generated by providing another pharmaceutical agent for diabetes to the patient. In any case, regardless of the disease, disorder, or condition being treated, the overall benefit experienced by the patient may be only the sum of the two pharmaceutical agents, or the patient may experience a synergistic benefit.

[0516] In any case, multiple drug agents (at least one of which is a compound disclosed herein) can be administered in any order or even simultaneously. If administered simultaneously, multiple drug agents can be provided in a single unified form or in multiple forms (as a single pill or as two separate pills, for example only). One of the drug agents can be administered in multiple doses, or two drug agents can be administered in multiple doses. If not administered simultaneously, the time between multiple doses can be any duration from a few minutes to eight weeks, or at any interval suitable for maintaining the desired therapeutic efficacy. In some embodiments, the time between multiple doses can be one minute, one hour, six hours, one day, two days, three days, four days, five days, six days, one week, two weeks, three weeks, four weeks, five weeks, six weeks, seven weeks or eight weeks.

[0517] Thus, in another aspect, certain embodiments provide methods for treating a p38MAP kinase-mediated disorder in a human or animal subject in need of such treatment, the method comprising administering to the subject an amount of a compound disclosed herein in combination with at least one additional agent known in the art for treating the disorder that is effective to reduce or prevent the disorder in the subject. In a related aspect, certain embodiments provide therapeutic compositions comprising at least one compound disclosed herein in combination with one or more additional agents for treating a p38MAP kinase-mediated disorder.

[0518] In additional embodiments, pharmaceutical compositions are provided that include one or more compounds of the present invention, one or more additional pharmaceutically active compounds, and a pharmaceutically acceptable carrier.

[0519] In other embodiments, the one or more additional pharmaceutically active compounds are selected from the group consisting of anti-inflammatory drugs, anti-atherosclerotic drugs, immunosuppressive drugs, immunomodulatory drugs, cytostatic drugs, antiproliferative agents, angiogenesis inhibitors, kinase inhibitors, cytokine blockers, and inhibitors of cell adhesion molecules.

[0520] The p38 MAP kinase inhibitor compositions described herein are also optionally used in combination with other therapeutic agents, which are selected based on the therapeutic value of the condition to be treated. Generally, in embodiments employing combination therapy, the pharmaceutical compositions described herein and other agents do not have to be administered in the same pharmaceutical composition, and are optionally administered by different routes due to different physical and chemical properties. Initial administration is usually performed according to an established regimen, and the dosage, mode of administration, and time of administration are subsequently changed based on the observed effects. In some cases, it is appropriate to administer the p38 MAP kinase inhibitor compositions described herein in combination with additional therapeutic agents. By way of example only, if one of the side effects experienced by a patient when receiving the p38 MAP kinase inhibitor compositions described herein is a rash, it is appropriate to administer an antihistamine agent in combination with the initial therapeutic agent. Alternatively, by way of example only, the therapeutic effect of the p38 MAP kinase inhibitor is enhanced by the administration of an additional pharmaceutical agent (which also comprises a therapeutic regimen) that also has a therapeutic benefit. In any case, regardless of the disease, disorder, or condition being treated, the overall benefit experienced by the patient is simply the sum of the two therapeutic agents, or the patient experiences a synergistic benefit.

[0521] When drugs are used in a therapeutic combination, the therapeutically effective doses vary. Methods for experimentally determining therapeutically effective doses of drugs and other agents used in a combination therapy regimen are documented methodologies. Combination therapy also includes periodic treatments that are started and stopped at various times to assist in the clinical management of the patient. In any case, the multiple therapeutic agents (one of which is a p38 MAP kinase inhibitor as described herein) are administered in any order or even simultaneously. If administered simultaneously, the multiple therapeutic agents are optionally provided in a single unified form or in multiple forms (by way of example only, as a single pill or as two separate pills).

[0522] In some embodiments, one of the therapeutic agents is administered in multiple doses, or both therapeutic agents are administered in multiple doses. If not administered simultaneously, the time between multiple doses optionally varies from more than zero weeks to less than twelve weeks.

[0523] In addition, combined methods and compositions are not limited to using only two medicaments, and the use of multiple therapeutic combinations is also contemplated. It should be understood that the dosage regimen for treating, preventing or improving one or more conditions for which relief is sought may be optionally changed according to a variety of factors. These factors include the disorder suffered by the subject, as well as the age, weight, sex, diet and physical condition of the subject. Therefore, in some embodiments, the dosage regimen actually used varies over a wide range, and is therefore different from the dosage regimen set forth herein.

[0524] The pharmaceutical agent that forms the combination therapy disclosed herein is optionally a combination dosage form or a separate dosage form that is intended to be administered substantially simultaneously. The pharmaceutical agent that forms the combination therapy is also optionally administered sequentially, wherein any of the agents is administered by requiring a two-step administration regimen. The two-step administration regimen optionally requires the sequential administration of active agents or the interval administration of active agents alone. According to the properties of each pharmaceutical agent (such as the efficacy, solubility, bioavailability, plasma half-life and kinetic properties of the pharmaceutical agent), the scope of the time period between multiple administration steps is from a few minutes to a few hours. The circadian rhythm variation of the target molecule concentration is optionally used to determine the optimal dose interval.

[0525] In other embodiments, the p38 MAP kinase inhibitor is optionally used in conjunction with a program that provides additional benefits or synergistic benefits to the patient. Before the occurrence of a disease or condition, during the occurrence of a disease or condition, or after the occurrence of a disease or condition, the p38 MAP kinase inhibitor and one or more additional therapies are optionally administered, and in some embodiments, the time of administering the composition containing the p38 MAP kinase inhibitor is different. Thus, for example, the p38 MAP kinase inhibitor is used as a prophylactic and is continuously administered to a subject with a tendency to develop a condition or disease to prevent the occurrence of the disease or condition. The p38 MAP kinase inhibitor and composition are optionally administered to the subject during or immediately after the onset of symptoms. Although embodiments of the present invention have been shown and described herein, it will be apparent to those skilled in the art that such embodiments are provided only by way of example. Without departing from the present invention, those skilled in the art will then think of many changes, modifications, and substitutions. It should be understood that in some embodiments of the present invention, various alternatives to the embodiments described herein are employed in practicing the present invention.

[0526] The p38MAP kinase inhibitors can be administered in combination with drugs from the following classes: NSAIDs, immunosuppressive drugs, immunomodulatory drugs, cytostatic drugs, antiproliferative agents, angiogenesis inhibitors, biologics, steroids, vitamin D3 analogs, retinoids, other kinase inhibitors, cytokine blockers, corticosteroids, and inhibitors of cell adhesion molecules. In the case where the subject suffers from atherosclerosis or a condition associated with atherosclerosis or the subject is at risk of suffering from atherosclerosis or a condition associated with atherosclerosis, the p38MAP kinase inhibitor compositions described herein are optionally used in any combination with one or more agents or methods for treating atherosclerosis or a condition associated with atherosclerosis. Examples of therapeutic agents / treatments for treating atherosclerosis or conditions associated with atherosclerosis include, but are not limited to, any of the following: torcetrapib, aspirin, niacin, HMG CoA reductase inhibitors (e.g., atorvastatin, fluvastatin, lovastatin, pravastatin, rosuvastatin, and simvastatin), colesevelam, cholestyramine, colestipol, gemfibrozil, probucol, and clofibrate.

[0527] Where the subject has or is at risk of having an inflammatory condition, the p38 MAP kinase inhibitor compositions described herein are optionally used in any combination with one or more agents or methods for treating the inflammatory condition.

[0528] Specific non-limiting examples of possible combination therapies include the use of a compound of the embodiments herein with a chemotherapeutic or anti-proliferative agent, an anti-inflammatory agent, an immunomodulatory or immunosuppressive agent, a neurotrophic factor, an agent for treating cardiovascular disease, an agent for treating diabetes, or an agent for treating an immunodeficiency disorder.

[0529] Specific non-limiting examples of possible combination therapies for inflammation include the use of certain compounds of the present disclosure with: (1) corticosteroids, including but not limited to cortisone, dexamethasone, and methylprednisolone; (2) nonsteroidal anti-inflammatory drugs (NSAIDs), including but not limited to ibuprofen, naproxen, acetaminophen, aspirin, fenoprofen (NALFON); TM ), Flurbiprofen (ANSAID TM ), ketoprofen, oxaprozin (DAYPROTM )、Diclofenac Sodium (VOLTAREN TM )、Diclofenac Potassium (CATAFLAM TM ), etodolac (LODINE TM ), indomethacin TM ), TORADOL TM ), sulindac (CLINORIL TM ), TOLECTIN TM )、Meclofenamic acid (MECLOMEN TM ), mefenamic acid (PONSTEL TM ), nabumetone (RELAFEN TM ) and piroxicam (FELDENE TM ); (3) immunosuppressants, including but not limited to methotrexate (RHEUMATREX TM ), leflunomide (ARAVA TM ), azathioprine (IMURAN TM ), cyclosporine (NEORAL TM 、SANDIMMUNE TM ), tacrolimus and cyclophosphamide (CYTOXAN TM ); (4) CD20 blockers, including but not limited to rituximab (RITUXAN TM ); (5) tumor necrosis factor (TNF) blockers, including but not limited to etanercept (ENBREL TM ), infliximab (REMICADE TM ) and adalimumab (HUMIRA TM ); (6) interleukin-1 receptor antagonists, including but not limited to anakinra (KINERET TM ); (7) interleukin-6 inhibitors, including but not limited to tocilizumab (ACTEMRA TM ); (8) interleukin-17 inhibitors, including but not limited to AIN457; (9) Janus kinase inhibitors, including but not limited to tasocitinib; and (10) SYK inhibitors, including but not limited to fostamatinib.

[0530] Specific non-limiting examples of possible combination therapies for the treatment of cancer include the use of certain compounds of the present disclosure with: (1) alkylating agents, including but not limited to cisplatin (PLATIN TM ), carboplatin (PARAPLATIN TM), oxaliplatin (ELOXATIN TM ), ZANOSAR TM ), busulfan (MYLERAN TM ) and cyclophosphamide (ENDOXAN); (2) antimetabolites, including but not limited to mercaptopurine (PURINETHOL TM ), thioguanine, pentostatin (NIPENT TM ), cytosine arabinoside (ARA-C TM ), gemcitabine (GEMZAR TM ), fluorouracil (CARAC TM ), folinic acid (FUSILEV TM ) and methotrexate (RHEUMATREX TM ); (3) plant alkaloids and terpenoids, including but not limited to vincristine (ONCOVIN TM ), vinblastine and taxol (TAXOL TM ); (4) topoisomerase inhibitors, including but not limited to irinotecan (CAMPTOSAR TM ), Topotecan (HYCAMTIN TM ) and etoposide (EPOSIN TM ); (5) cytotoxic antibiotics, including but not limited to actinomycin D (COSMEGEN TM ), ADRIAMYCIN TM ), bleomycin (BLENOXANE TM ) and mitomycin (MITOSOL TM ); (6) angiogenesis inhibitors, including but not limited to sunitinib (SUTENT TM ) and bevacizumab (AVASTIN TM ); (7) tyrosine kinase inhibitors, including but not limited to imatinib (GLEEVEC TM ), erlotinib (TARCEVA TM ), lapatinib (TYKERB TM ) and axitinib (INLYTA TM ); and (8) immune checkpoint inhibitors, including but not limited to atezolizumab (TECENTRIQ TM ), avelumab (BAVENCIO TM )、durvalumab (IMFINZI TM ), ipilimumab (YERVOY TM), pembrolizumab (KEYTRUDA TM ), nivolumab (OPDIVO TM ), tremelimumab; and combinations thereof (e.g., FOLFIRINOX (which is a combination of folinic acid (also known as folinic acid), fluorouracil, irinotecan, and oxaliplatin)).

[0531] In some embodiments, the compounds disclosed in the embodiments herein may also be co-administered (simultaneously or sequentially) with a variety of other pharmaceutical agents or treatments, e.g., pharmaceutical agents or treatments that are administered systemically (e.g., orally or parenterally). Examples of such systemic treatments include topical or systemic corticosteroids (e.g., prednisone), antibiotics (e.g., erythromycin, tetracycline, and dicloxacillin), antifungal agents (e.g., those sold under the trade name Diflucan TM ketoconazole and fluconazole), antivirals (such as those sold under the brand name Valtrex TM Valacyclovir, acyclovir, and Famvir sold under the brand name TM famciclovir, sold under the brand name Cytoxan TM Cyclophosphamide, azathioprine, methotrexate, mycophenolate mofetil, biologics (such as Rituxan TM Rituximab is sold under the brand name Enbrel TM Etanercept is sold under the brand name Humira TM Adalimumab is sold under the brand name Remicade TM Infliximab is sold under the brand name Stelara TM Ustekinumab, sold under the brand name Amevive TM alefacept, which is sold on the market, and / or thyroid hormone replacement.

[0532] In some embodiments, other therapies that can be used in combination with the compounds disclosed herein include, for example, mercaptopurine, topical or systemic corticosteroids (such as prednisone, methylprednisolone and prednisolone), alkylating agents (such as cyclophosphamide), calcineurin inhibitors (such as cyclosporine, sirolimus and tacrolimus), inhibitors of inosine monophosphate dehydrogenase (IMPDH) (such as mycophenolate mofetil, mycophenolate mofetil, azathioprine), various antibodies (e.g., anti-lymphocyte globulin (ALG), anti-thymocyte globulin (ATG), monoclonal anti-T cell antibody (OKT3)), and radiation. These various agents can be used according to their standard doses or commonly used doses (as specified in the prescribing information accompanying the commercially available drug form (see also the prescribing information in The Physician's Desk Reference (2006 edition)). In some embodiments, the standard doses of these agents can be reduced when used in combination with the compounds of the embodiments herein. Without limiting the scope of the present disclosure, it is believed that such a combination can produce a synergistic result with better efficacy, less toxicity, longer duration of action, or faster response to therapy. In some embodiments, the combination therapy of the embodiments herein can be administered in the form of a subtherapeutic amount of a compound of the embodiments herein, or an additional pharmaceutical agent, or both a compound of the embodiments herein and an additional pharmaceutical agent. Azathioprine is currently available under the brand name Azasan TM Available from Salix Pharmaceuticals, Inc.; mercaptopurine is currently available under the brand name Purinethol TM Available from Gate Pharmaceuticals, Inc.; prednisone and prednisolone are currently available from Roxane Laboratories, Inc.; methylprednisolone is currently available from Pfizer; sirolimus (rapamycin) is currently available under the brand name Rapamune TM Available from Wyeth-Ayerst; tacrolimus is currently available under the brand name Prograf TM Obtained from Fujisawa; cyclosporine is currently available under the brand name Sandimmune TM Acquired from Novartis and under the brand name Gengraf TM Obtained from Abbott; IMPDH inhibitors (such as mycophenolate mofetil and mycophenolic acid) are currently available under the brand name Cellcept TM Obtained from Roche and under the brand name Myfortic TM Obtained from Novartis; azathioprine is currently available under the brand name Imuran TMobtained from Glaxo Smith Kline; and the antibody is currently available under the brand name Orthoclone TM Obtained from Ortho Biotech under the brand name Simulect TM (basiliximab) from Novartis and under the brand name Zenapax TM (daclizumab) was obtained from Roche.

[0533] In some embodiments, the compound of the embodiments herein is administered in combination, concomitantly or adjunctively with the above pharmaceutical agents or therapies and / or with pharmaceutical agents or therapies for other diseases. For example, the compound of the embodiments herein can be combined with thyroid hormone replacement therapy or with anti-inflammatory therapy or immunomodulatory therapy.

[0534] In some embodiments, the combination therapy of the embodiments herein may be administered in the form of a subtherapeutic amount of a compound of the embodiments herein, or an additional pharmaceutical agent, or both a compound of the embodiments herein and an additional pharmaceutical agent.

[0535] For use in cancer and tumor diseases, the p38 MAP kinase inhibitor is best used with one or more of the following drug classes: wherein the anticancer agent is an EGFR kinase inhibitor, a MEK inhibitor, a VEGFR inhibitor, an anti-VEGFR2 antibody, a KDR antibody, an AKT inhibitor, a PDK-1 inhibitor, a PI3K inhibitor, a c-kit / Kdr tyrosine kinase inhibitor, a Bcr-Abl tyrosine kinase inhibitor, a VEGFR2 inhibitor, a PDGFR-β inhibitor, a KIT inhibitor, a Flt3 tyrosine kinase inhibitor, a PDGF receptor family inhibitor, a Flt3 tyrosine kinase inhibitor, a RET tyrosine kinase receptor family inhibitor, a VEGF-3 receptor antagonist, a Raf protein kinase family inhibitor, an angiogenesis inhibitor, an Erb2 inhibitor, an mTOR inhibitor, an IGF-1R antibody, a NFkB inhibitor, a proteasome inhibitor, a chemotherapeutic agent or a glucose reducing agent.

[0536] In any case, multiple drug agents (at least one of which is a compound disclosed herein) can be administered in any order or even simultaneously. If administered simultaneously, multiple drug agents can be provided in a single unified form or in multiple forms (as a single pill or as two separate pills, for example only). One of the drug agents can be administered in multiple doses, or two drug agents can be administered in multiple doses. If not administered simultaneously, the time between multiple doses can be any duration from a few minutes to eight weeks, or at any interval suitable for maintaining the desired therapeutic efficacy. In some embodiments, the time between multiple doses can be one minute, one hour, six hours, one day, two days, three days, four days, five days, six days, one week, two weeks, three weeks, four weeks, five weeks, six weeks, seven weeks or eight weeks.

[0537] In certain embodiments, the additional pharmaceutical agent is selected from a taxane, an inhibitor of bcr-abl, an inhibitor of EGFR, a DNA damaging agent, an antimetabolite, paclitaxel, imatinib, dasatinib, nilotinib, erlotinib, gefitinib, cisplatin, oxaliplatin, carboplatin, anthracyclines, AraC, 5-FU, camptothecin, doxorubicin, idarubicin, paclitaxel, docetaxel, vincristine, a MEK inhibitor, U0126, a KSP inhibitor, vorinostat, pembrolizumab, nivolumab, atezolizumab, avelumab, tesimumab, and durvalumab.

[0538] In some embodiments, the composition further comprises an additional pharmaceutical agent selected from a chemotherapeutic or antiproliferative agent, an antiviral agent, an antibiotic, an antihistamine, a lubricant, systemic phototherapy, psoralen photochemotherapy, laser therapy, hormone replacement therapy, an anti-inflammatory agent, an immunomodulator or immunosuppressant, a neurotrophic factor, an agent for treating cardiovascular disease, an agent for treating diabetes, and an agent for treating an immunodeficiency disorder.

[0539] In some embodiments, one or more compounds of the embodiments herein can be used in combination with one or more other therapeutic agents used in the treatment of p38MAP kinase-mediated disorders, and the therapeutic response can be improved compared to the response to the other therapeutic agents alone without exacerbating their toxic effects. In some embodiments, the compounds of the embodiments herein can be used in combination with one or more JAK1 inhibitors and / or JAK3 inhibitors and / or JAK2 inhibitors and / or TYK2 inhibitors for the treatment of p38MAP kinase-mediated disorders. An additive effect or synergistic effect is the desired result of such a combination. The additional agent can be combined with the compounds of the present invention in a single dosage form or a continuous dosage form, or the agent can be administered simultaneously or sequentially as a separate dosage form. In some embodiments, one or more additional agents can be administered to a patient in combination with at least one p38MAP kinase inhibitor / antagonist described herein, wherein the additional agent is administered intermittently (rather than continuously).

[0540] General synthetic methods for preparing compounds

[0541] Some embodiments herein are directed to methods of maximizing the synthetic yield of a compound having a structure of Formula (P)-Ia (as described herein) or a derivative thereof by interconverting a compound having a structure of Formula (M)-Ib:

[0542]

[0543] in:

[0544] X is CH or N;

[0545] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0546] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0547] R 3 Selected from the group consisting of:

[0548]

[0549] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0550] R 5 Selected from H or C1-C3 alkyl;

[0551] m is 1 or 2;

[0552] n is 0 or 1;

[0553] p is 1;

[0554] q is 0 or 1; and

[0555] The method comprises heating a solution comprising a compound of Formula (M)-Ib to a temperature at which the compounds undergo interconversion.

[0556] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-Ia (as described herein) by interconverting a compound having a structure of Formula (M)-Ib, wherein the solution comprises a solvent that withstands an interconversion temperature of 110°C-170°C.

[0557] In further embodiments, methods are provided for maximizing the synthetic yield of compounds having the structure of Formula (P)-IIa (as described herein) or derivatives thereof by interconverting compounds having the structure of Formula (M)-IIb:

[0558]

[0559] in:

[0560] X is CH or N;

[0561] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0562] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0563] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0564] R 5 is H or C1-C3 alkyl;

[0565] m is 1 or 2;

[0566] n is 0 or 1; and

[0567] The method comprises heating a solution comprising a compound of Formula (M)-Ib to an interconversion temperature to form a mixture of atropisomers of the compound of Formula (M)-Ib and the compound of Formula (P)-Ia.

[0568] In other embodiments, the solution is heated to the interconversion temperature for 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, or a range between any two of these values.

[0569] In other embodiments, the solution is heated to the interconversion temperature for 3 hours.

[0570] In other embodiments, the interconversion temperature is from 110°C to 210°C.

[0571] In other embodiments, the interconversion temperature is from 110°C to 170°C.

[0572] In other embodiments, the interconversion temperature is from 110°C to 150°C.

[0573] In other embodiments, the interconversion temperature is 110°C to 140°C.

[0574] In other embodiments, the interconversion temperature is 110°C-125°C.

[0575] In other embodiments, the interconversion temperature is 145°C-150°C.

[0576] In other embodiments, the solvent is ethanol.

[0577] In further embodiments, the solvent is N-methylpyrrolidone, or dimethylacetamide, or a combination thereof.

[0578] In further embodiments, the solvent has a boiling point of 110°C-150°C.

[0579] In other embodiments, the solvent is ethylene glycol.

[0580] In other embodiments, the solvent is n-butanol.

[0581] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-Ia by interconverting a compound having a structure of Formula (M)-Ib, wherein the method comprises heating a solution comprising a compound of Formula (M)-Ib to the boiling point of a solvent.

[0582] In other embodiments, the solvent is ethanol.

[0583] In further embodiments, the solvent is N-methylpyrrolidone, or dimethylacetamide, or a combination thereof.

[0584] In further embodiments, the solvent has a boiling point of 110°C-150°C.

[0585] In other embodiments, the solvent is ethylene glycol.

[0586] In other embodiments, the solvent is n-butanol.

[0587] In additional embodiments, methods are provided for maximizing the synthetic yield of compounds having the structure of Formula (P)-Ia by adding a polar solvent to the interconverted mixture to precipitate the interconverted product including the compound of Formula (P)-Ia.

[0588] In other embodiments, the ratio (% V添加的 / V初始 ) Add a polar solvent to the mixture after interconversion.

[0589] In other embodiments, the polar solvent is added prior to cooling of the interconverted mixture.

[0590] In other embodiments, the polar solvent is added after cooling of the interconverted mixture.

[0591] In other embodiments, the polar solvent is water.

[0592] In other embodiments, water is added to about 75% v / v 添加的 / v 初始 .

[0593] In another embodiment, water is added to 80% v 添加的 / v 初始 .

[0594] In other embodiments, water is added to about 85% v / v 添加的 / v 初始 .

[0595] In further embodiments, the process of isolating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib is repeated multiple times.

[0596] In further embodiments, the process of isolating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib is repeated one, two, three, or four times.

[0597] In further embodiments, the process of isolating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib is repeated twice.

[0598] In additional embodiments, the method of separating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib produces a ratio of about 2:1, about 3:1, about 4:1, about 5:1, about 6:1, about 7:1, about 8:1, about 9:1 or about 10:1 of compounds having the structure of Formula (P)-Ia, substantially free of the corresponding M isomer, to compounds having the structure of Formula (M)-Ib, substantially free of the corresponding P isomer.

[0599] In another embodiment, the method of separating compounds of Formula (P)-Ia by interconverting compounds of Formula (M)-Ib produces a ratio of approximately 5:1 of a compound having a structure of Formula (P)-Ia substantially free of the corresponding M isomer to a compound having a structure of Formula (M)-Ib substantially free of the corresponding P isomer.

[0600] Non-limiting examples of compounds of formula (M)-IIb include the following compounds or derivatives thereof:

[0601]

[0602]

[0603]

[0604]

[0605]

[0606]

[0607]

[0608]

[0609]

[0610]

[0611]

[0612]

[0613] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-IIIa by interconverting a compound having a structure of Formula (M)-IIIb or a derivative thereof:

[0614]

[0615] in:

[0616] X is CH or N;

[0617] R 1 is chlorine or bromine;

[0618] R 2 is -H or methyl; and

[0619] Wherein the method comprises heating a solution comprising a compound of formula (M)-IIIb to a temperature at which the compound interconverts. Non-limiting examples of compounds of formula (M)-IIIb include the following compounds or derivatives thereof:

[0620]

[0621]

[0622] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-IVa by interconverting a compound having a structure of Formula (M)-IVb or a derivative thereof:

[0623]

[0624] in:

[0625] X is CH or N;

[0626] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0627] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0628] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0629] R 5 is H or C1-C3 alkyl;

[0630] m is 1 or 2;

[0631] n is 0 or 1; and

[0632] The method comprises heating a solution comprising a compound of formula (M)-IVb to a temperature at which the compounds interconvert.

[0633] In other embodiments, methods are provided for maximizing the synthetic yield of compounds having a structure of Formula (P)-Va by interconverting compounds having a structure of Formula (M)-Vb or derivatives thereof:

[0634]

[0635] in:

[0636] X is CH or N;

[0637] R 1 is chlorine or bromine;

[0638] R 2 is H or methyl; and

[0639] Wherein the method comprises heating a solution comprising a compound of Formula (M)-Vb to a temperature at which the compounds interconvert.

[0640] Non-limiting examples of compounds of formula (M)-Vb include the following compounds and their derivatives:

[0641]

[0642]

[0643]

[0644] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-VIa by interconverting a compound having a structure of Formula (M)-VIb or a derivative thereof:

[0645]

[0646] in:

[0647] X is CH or N;

[0648] R 1 Selected from the group consisting of H, C1-C6 alkyl, fluorine, chlorine, bromine, cyano or -CF3;

[0649] R 2 selected from the group consisting of H, methyl, cyano or fluorine;

[0650] R 4 Selected from the group consisting of H, methyl, OH and -OCH3;

[0651] R 5 is H or C1-C3 alkyl;

[0652] p is 1;

[0653] q is 0 or 1; and

[0654] The method comprises heating a solution comprising a compound of Formula (M)-VIb to a temperature at which the compounds interconvert.

[0655] In another embodiment, a method is provided for maximizing the synthetic yield of a compound having a structure of Formula (P)-VIIa by interconverting a compound having a structure of Formula (M)-VIIb or a derivative thereof:

[0656]

[0657] in:

[0658] X is CH or N;

[0659] R 1 is chlorine or bromine;

[0660] R 2 is -H or methyl; and

[0661] The method comprises heating a solution comprising a compound of Formula (M)-VIb to a temperature at which the compounds interconvert.

[0662] Non-limiting examples of compounds of formula (M)-VIIb include the following compounds and their derivatives:

[0663]

[0664]

[0665]

[0666] In certain embodiments, each of the compounds of Formula (M)-Ib, Formula (M)-IIb, Formula (M)-IIIb, Formula (M)-IVb, Formula (M)-Vb, Formula (M)-VIb, and Formula (M)-VIIb is substantially free of its corresponding P isomer.

[0667] The compounds of the present invention can be prepared using the methods described in the general synthesis schemes and experimental procedures described in detail below. The general synthesis schemes and experimental procedures are presented for illustrative purposes and are not intended to be restrictive. The starting materials for preparing the compounds of the present invention are commercially available or can be prepared using conventional methods known in the art. Representative procedures for preparing the compounds of the present invention are outlined in Schemes 1 and 2 below. Solvents and reagents for the synthesis preparation not described below can be purchased at Sigma-Aldrich or Fisher Scientific.

[0668] The compounds of the present invention can be prepared using the methods illustrated in the general synthetic schemes and experimental procedures described in detail below. These general synthetic schemes and experimental procedures are presented for illustrative purposes and are not intended to be restrictive. The starting materials used to prepare the compounds of the present invention are commercially available or can be prepared using conventional methods known in the art.

[0669] The exemplary method of maximizing yield by conformational interconversion is also illustrated below. It is not intended to be restrictive that exemplary solvents, solvent mixtures and azeotropes are used for interconversion by reflux. It is not intended to be restrictive that exemplary solvents, solvent mixtures, azeotropes and processes used for interconversion or recovery of product or reduction of residual solvent level are not intended to be restrictive. Any solvent or solvent mixture with appropriate thermal properties, ICH Class II or above, low residual solvent level and some mechanisms that allow product recovery after interconversion are suitable for the purpose of the method. Using some solvents may cause more impurities than other solvents in the mixture after racemization. Preferably, solvents of the present disclosure have a more desirable relative impurity distribution.

[0670] With a T higher than the interconversion 起始 Any solvent or azeotrope having a boiling point of 100°C and below the decomposition temperature would be preferred.

[0671] For example, compound 49b has a decomposition temperature range of 240°C-300°C and was found to interconvert in NMP at 138°C. The boiling point of NMP is 138°C (at 1 atm); which is lower than the decomposition temperature range of compound 49b. Therefore, NMP would be a preferred solvent for interconversion of compound 49b.

[0672] In another example, compound 49b was found to interconvert in ethylene glycol at ~114°C. The boiling point of ethylene glycol is 197°C (at 1 atm); below the decomposition temperature range of compound 49b. Therefore, ethylene glycol would be a preferred solvent for the interconversion of compound 49b.

[0673] ICH Class II or Class III includes any solvent or azeotrope that is non-genotoxic in animals, or at most, a possible cause of irreversible toxicity that meets this criterion. These solvents or azeotropes may be suspected of having other significant but reversible toxicities and meet this criterion. The entire ICH Q3C series of guidance publications are incorporated by reference.

[0674] Class II solvents: acetonitrile, chlorobenzene, chloroform, cumene, cyclohexane, 1,2-dichloroethylene, dichloromethane, 1,2-dimethoxyethane, N,N-dimethylacetamide, N,N-dimethylformamide, 1,4-dioxane, 2-ethoxyethanol, ethylene glycol, formamide, hexane, methanol, 2-methoxyethanol, methyl butyl ketone, methylcyclohexane, N-methylpyrrolidone, nitromethane, pyridine, sulfolane, tetrahydrofuran, tetralin, toluene, 1,1,2-trichloroethylene and xylene.

[0675] ICH Class III: Any solvent or azeotrope that is not considered to be hazardous to human health at levels normally accepted in pharmaceuticals.

[0676] Class III solvents: acetic acid, acetone, anisole, 1-butanol, 2-butanol, butyl acetate, tert-butyl methyl ether, dimethyl sulfoxide, ethanol, ethyl acetate, ethyl ether, ethyl formate, formic acid, heptane, isobutyl acetate, isopropyl acetate, methyl acetate, 3-methyl-1-butanol, methyl ethyl ketone, methyl isobutyl ketone, 2-methyl-1-propanol, pentane, 1-pentanol, 1-propanol, 2-propanol, and propyl acetate.

[0677] Any solvent or azeotrope that produces a drug product containing no more residual solvents than the safety data can support would be preferred. Based on the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines, specifically option 1 in the Q3C (R5) Impurities: Residual Solvents guidelines (assuming 10 g or less per day of administration). This document is incorporated by reference.

[0678] For example, the maximum limit for ethylene glycol is 620 ppm.

[0679] As a further example, NMP has a maximum limit of 530 ppm.

[0680] Any solvent, solvent mixture or azeotrope with the characteristic that allows to recycle the product after interconversion will be preferred. For example, adding a polar solvent to the mixture after interconversion can precipitate a certain amount of interconverted product. Certain ratios of polar solvent to interconversion solvent can provide higher precipitation level of the interconverted product.

[0681] For example, compound 49b can be interconverted using ethylene glycol, and pure water is added to the interconverted mixture including ethylene glycol to about 75% v / v (based on the starting solution), allowing precipitation of at least 75-85% of the interconverted product.

[0682] In another example, when compound 49b is interconverted in NMP, the addition of purified water to the interconverted mixture including NMP allows precipitation of about at least 70% of the interconverted product.

[0683] In the present invention, the interconversion compounds are balanced, no or relatively few impurities are produced, and no or relatively few mass balance losses are produced. For example, based on TGA and DSC of exemplary compound 49b, the destructive melting decomposition starting temperature is about 240°C-300°C. Based on TGA and DSC of exemplary compound 49b, the interconversion temperature based on dry solid is about 170°C-208°C. However, for exemplary compound 49b in ethylene glycol, the interconversion temperature (or T of interconversion) based on liquid is about 170°C-208°C. 起始) is ~113 ℃. For exemplary compound 49b, the gap between the dry solid interconversion temperature and the destructive melting decomposition temperature is about 20 ℃, while the gap between the liquid-based interconversion temperature and the destructive melting decomposition temperature in ethylene glycol is 125 ℃. The increase in the gap between the dry solid interconversion temperature and the destructive melting decomposition onset temperature to the gap between the liquid-based interconversion temperature and the destructive melting decomposition onset temperature is unexpected. In addition, the interconversion temperature in NMP is about 138 ℃, which provides a gap of about 67 ℃ between the interconversion and destructive decomposition temperatures. This is another example of an increase in the workable temperature gap relative to the interconversion temperature based on dry solids.

[0684] The present disclosure outlines a method for significantly increasing the recovery of atropisomers with negative optical rotation by recycling discarded atropisomers with positive optical rotation without modifying the original synthesis outlined in Schemes 1 or 2. Such recycling will greatly increase the cumulative maximum recovery of atropisomers with negative optical rotation with relatively few unit operations.

[0685] Representative Synthesis Methods

[0686] Representative procedures for preparing compounds of the present disclosure are outlined in Schemes 1 and 2. Substituted pyridine starting materials, which are provided as intermediates, can be purchased or prepared using methods known in the art with representative procedures. Scheme 1 highlights the synthesis of 1,4'-bipyridin-2-one in full detail. The synthesis of pyridone 1c can be accomplished by reaction of acetal 1a and pyridine 1b in a solvent such as dioxane. Substitution of the desired heteroaryl substituent (R 3 ) alkylation of phenol 1c affords alkylated 1d. 2 and X-substituents, pyridone 1d can be converted to the title compound in one of three ways. For example, if R 2 If X is methyl, then reaction of 1d with a vinyltin reagent in the presence of a palladium catalyst provides the methyl ketone 1i. Halogenation of 1i using N-chlorosuccinimide (or N-bromosuccinimide if the corresponding bromine is desired) in a solvent such as isopropanol provides 1j. In situ enamine formation by reaction of 1j with N,N-dimethylformamide dimethyl acetal provides an intermediate which is subsequently reacted with 2-hydroxy-2-methylpropionamidine in a solvent such as DMF to produce the pyridinone 1g. Alternatively, if X is N, then 1d can be carboxylated by treating the halide with carbon monoxide in ethanol in the presence of a palladium catalyst to produce the ester 1e. Hydrolysis of the ester 1e with lithium hydroxide in water followed by treatment of the intermediate carboxylic acid with CDI and subsequent treatment with methoxymethylamine and an amine base such as diisopropylethylamine under Weinreb conditions produces 1h. The Weinreb amide 1h reacts with the desired R 2Reaction of the Grinard reagent in a solvent such as THF provides 1i. The ketone 1i is then converted to 1j and, as shown above, subsequently converted to the final compound 1g. Another option for setting the pyridine or pyrimidine D ring is to react 1d with the desired boronic acid using an appropriate palladium catalyst under Suzuki conditions to produce a coupling intermediate, which is then halogenated using NCS or NBS to provide 1f. Addition of methylmagnesium bromide to 1f in a solvent such as THF provides the title compound 1g. The resulting equilibrium mixture of atropisomers can be resolved by supercritical fluid chromatography using a mobile phase of carbon dioxide and ethanol. The individual atropisomer conformations can then be interconverted by dissolving in a solvent such as NMP or ethylene glycol and heating to an appropriate internal temperature for the interconversion. The product is recovered by adding water to the interconverted solution and precipitating, and then the product is resolved by supercritical fluid chromatography using a mobile phase of carbon dioxide and ethanol.

[0687] Solution 1:

[0688]

[0689] Scheme 2 shows that the benzyl substituent R is added in the last step. 3 Synthesis of the desired compound. As described in Scheme 1, pyridone 2c can be achieved by reaction of acetal 2a and pyridine 2b in a solvent such as dioxane. Protection of the phenol of 2c with p-methoxybenzyl bromide produces benzylated 2d. Reaction of 2d with a vinyl tin reagent in the presence of a palladium catalyst provides methyl ketone 2e. Halogenation of 1e using N-chlorosuccinimide (or N-bromosuccinimide if the corresponding bromine is desired) in a solvent such as isopropanol provides 2f. In situ enamine formation by reaction of 2f with N,N-dimethylformamide dimethyl acetal provides an intermediate, which is subsequently reacted with 2-hydroxy-2-methylpropionamidine in a solvent such as DMF to produce pyrimidone 2g. Deprotection of the benzyl group by treating 2g with an acid such as TFA or HCl provides 2h. Reaction with the desired benzyl halide substituent (R 3 CH2Br or R 3 CHCl) alkylation of phenol 2h provides the desired pyridone 2i. The resulting atropisomer equilibrium mixture can be resolved by supercritical fluid chromatography with a mobile phase of carbon dioxide and ethanol. The single atropisomer conformation can then be interconverted by dissolving in a solvent (such as NMP or ethylene glycol) and being heated to a suitable internal temperature for interconversion. The product is recovered by adding water to the solution after the interconversion and precipitating, and then the product is resolved by supercritical fluid chromatography with a mobile phase of carbon dioxide and ethanol.

[0690] Solution 2:

[0691]

[0692] Example 1: Synthesis of Compound 49a

[0693] Preparation of 2-chloromethyl-3,5-difluoro-pyridine

[0694]

[0695] Step A: Preparation of 3,5-difluoro-pyridine-2-carboxylic acid ethyl ester

[0696]

[0697] Using ice-water bath cooling, thionyl chloride (2 mL) was added dropwise to a suspension of 3,5-difluoropyridine-2-carboxylic acid (2.0 g, 12.6 mmol) in ethanol (5 mL). The solution was heated at 60 ° C for 3 h. The reaction was returned to ambient temperature and concentrated in vacuo to provide ethyl ester hydrochloride (2.5 g) as a yellow oil.

[0698] Step B: Preparation of (3,5-difluoro-pyridin-2-yl)-methanol

[0699]

[0700] Using ice-water bath cooling, sodium borohydride (1.43g, 37.8mmol) solution of 3,5-difluoro-pyridine-2-carboxylic acid ethyl ester (2.5g, 12.6mmol) of part A in ethanol (10mL) was added in batches. The solution was stirred at 0°C for thirty minutes and at ambient temperature for 2h. The reaction returned to 0°C and saturated ammonium chloride was added dropwise. The solvent was removed in a vacuum and the resulting residue was distributed between ethyl acetate and water. The organic layer was washed with saturated ammonium chloride, water and brine, and dried over magnesium sulfate. The slurry was filtered and concentrated to provide alcohol (1.8g) as a yellow oil: MS (ES) m / e146 (M+H).

[0701] Step C: Preparation of 2-chloromethyl-3,5-difluoro-pyridine

[0702]

[0703] To a solution of (3,5-difluoro-pyridin-2-yl)-methanol (1.8 g, 12.3 mmol) from Part B in dichloromethane (20 mL) was added three drops of N,N-dimethylformamide and cooled using an ice-water bath. Thionyl chloride (2 mL) was added dropwise and the solution was stirred at ambient temperature for one hour. The solution was concentrated in vacuo to provide the chloro compound (1.75 g) as a light brown liquid.

[0704] Compound 49a: Preparation of 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0705]

[0706] Step A: Preparation of 2'-chloro-4-hydroxy-6,5'-dimethyl-[1,4']bipyridinyl-2-one

[0707]

[0708] To a screw cap vial with a rubber septum was added 2,2-dimethyl-6-(2-oxo-propyl)-[1,3]dioxin-4-one (prepared as described in Organic Letters, 11(21), 4910-4913; 2009) (500 mg, 2.7 mmol) and 2-chloro-5-methyl-pyridin-4-ylamine (575 mg, 4 mmol, 1.5 equiv). The mixture was dissolved in anhydrous 1,4-dioxane (10 mL). Once the mixture was uniform, the vial was placed on a stirrer / hot plate preset to 90°C. The reaction vessel was heated at this temperature for 3.5 h. The reaction vial was removed from the heat source and analyzed by HPLC, which showed that the reaction was >95% complete. The vial was placed back on the hot plate. H2SO4 (250 μL) was added to the heated mixture and the reaction was heated for 1 h. The reaction bottle was removed from the heat source and, after cooling to ambient temperature, dioxane was removed by passing air stream through the top of the open bottle to produce a brown residue. Water (~4 mL) was added to the bottle and the mixture was stirred for 30 min. The resulting tan solid was washed and filtered out with additional water and diethyl ether to produce the desired product (531 mg, 57% based on sulfate) as a tan solid with a purity of ~95% by HPLC: MS (ES) m / e 250 (M+H).

[0709] Step B: Preparation of 2'-chloro-4-((4-methoxybenzyl)oxy)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0710]

[0711] To a solution of 2'-chloro-4-hydroxy-6,5'-dimethyl-[1,4']bipyridinyl-2-one (6.0 g, 20.1 mmol) of Part A in N,N-dimethylformamide (20 mL) was added 4-methoxybenzyl chloride (2.73 mL, 20.1 mmol), potassium carbonate (6.93 g, 50.2 mmol) and 18-crown-6 (100 mg). The slurry was heated at 60 ° C for 3 h and stirred at ambient temperature for 18 h. The reaction mixture was partitioned between ethyl acetate and water. The organic layer was washed with water and brine and dried over magnesium sulfate. The solution was concentrated in vacuo to provide a brown oil. Normal phase chromatography (ethyl acetate / heptane) provided the alkylated product (4.6 g) as a pale yellow solid: MS (ES) m / e 371 (M+H).

[0712] Step C: Preparation of 2'-acetyl-4-((4-methoxybenzyl)oxy)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0713]

[0714] Using CEM Explorer at 130°C TM A solution of 2'-chloro-4-((4-methoxybenzyl)oxy)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (4.6 g, 12.4 mmol), tributyl(1-ethoxyethylene)tin (4.6 mL, 13.6 mmol) and PdCl2(PPh3)2 (87 mg, 0.12 mmol) of Part B in 1,4-dioxane (30 mL) was microwaved for 2 h. The resulting dark solution was filtered through diatomaceous earth rinsed with ethyl acetate. The filtrate was concentrated and the residue was dissolved in tetrahydrofuran (5 mL) and treated with concentrated HCl until hydrolysis was complete. The solution was concentrated in vacuo and purified using normal phase chromatography (ethyl acetate / heptane) to provide the acetyl compound (3.3 g) as a yellow oil: MS (ES) m / e 379 (M+H).

[0715] Step D: Preparation of 2'-acetyl-3-chloro-4-hydroxy-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0716]

[0717] To a solution of 2'-acetyl-4-((4-methoxybenzyl)oxy)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (3.3 g, 8.7 mmol) of Part C in 2-propanol (100 mL) was added N-chlorosuccinimide (1.27 g, 9.6 mmol) and 10 drops of dichloroacetic acid. The slurry was heated at 60°C for 3 h. The resulting slurry was concentrated and the residue was partitioned between ethyl acetate and water. The organic layer was washed with water and brine and dried over magnesium sulfate. The solution was concentrated in vacuo. The residue was suspended in dichloromethane and the resulting white solid was collected by vacuum filtration to provide the chlorinated deprotected product (1.16 g): MS (ES) m / e 293 (M+H).

[0718] Step E: Preparation of 2'-acetyl-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one

[0719]

[0720] To a solution of 2'-acetyl-3-chloro-4-hydroxy-5',6-dimethyl-2H-[1,4'-bipyridine]-2-one (500 mg, 1.7 mmol) of part D in N,N-dimethylformamide (3 mL) was added 2-chloromethyl-3,5-difluoro-pyridine (277 mg, 1.7 mmol), potassium carbonate (590 mg, 4.28 mmol) and 18-crown ether-6 (10 mg), and the reaction was stirred at 60 ° C for 4 h. After cooling, the solution was partitioned between ethyl acetate and water. The organic layer was washed with water and brine, and dried over magnesium sulfate. The solution was filtered and concentrated in vacuo. The crude material was purified using normal phase chromatography (ethyl acetate / heptane) to provide an alkylated product (397 mg) as a yellow solid: MS (ES) m / e 420 (M+H).

[0721] Step F: Preparation of (±)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0722]

[0723] To a solution of 2'-acetyl-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (397 mg, 0.95 mmol) from step E in N,N-dimethylformamide (3 mL) was added N,N-dimethylformamide dimethyl acetal (0.18 mL, 1.42 mmol) and the solution was heated to 55° C. for 18 h. The solution was concentrated to half the volume and 2-hydroxy-2-methylpropionamidine HCl (195 mg, 1.42 mmol) and potassium carbonate (393 mg, 2.85 mmol) were added. The slurry was heated at 75° C. for 18 h. The slurry was returned to ambient temperature and distributed between ethyl acetate and water. The organic layer was washed with brine and dried over magnesium sulfate. The solution was concentrated and purified using normal phase chromatography (ethyl acetate / heptane) to provide the title compound (255 mg, 46%) as a light yellow solid: MS (ES) m / e 514 (M+H).

[0724] Chiral resolution of 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0725] Racemic 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (250 mg, 0.49 mmol) was separated using supercritical fluid chromatography (Thar 80, preparative SFC, ChiralCel OD-H, 250×30 mm ID column) with a mobile phase of carbon dioxide and ethanol. The separation method used an isocratic method with 40% ethanol at a flow rate of 50 mL / min and a cycle time of 10 min. Optical rotation was determined using a WZZ-2S polarimeter.

[0726] The faster isomer eluted at 1.77 minutes, yielding 115 mg of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one in ethylene glycol: [α] D 20 -46°(CH3OH); 1H NMR(400MHz,DMSO-d6)δppm 8.97(d,J=5.09Hz,1H),8.86(s,1H),8.69(s,1H),8.61(s,1H),8.24(d,J=5.08Hz,1H),8.10(t,1H),6.85( s,1H),5.50(s,2H),5.26(s,1H),2.11(s,3H),1.98(s,3H),1.54(s,3H),1.52(s,3H); MS(ES)m / e514(M+H).

[0727] The slower isomer eluting at 3.68 minutes yielded 112 mg of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one in ethylene glycol: [α] D 20 +45°(CH3OH); 1 H NMR(400MHz,DMSO-d6)δppm 8.97(d,J=5.09Hz,1H),8.86(s,1H),8.69(s,1H),8.61(s,1H),8.24(d,J=5.08Hz,1H),8.10(t,1H),6.85( s,1H),5.50(s,2H),5.26(s,1H),2.11(s,3H),1.98(s,3H),1.54(s,3H),1.52(s,3H); MS(ES)m / e514(M+H).

[0728] Conformational interconversion of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one in ethylene glycol.

[0729] In a 4-neck RBF equipped with a mechanical stirrer, a thermometer bag, a reflux condenser and a 10-liter oil bath for heating, (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (40g, 77.8mmol) was suspended in ethylene glycol (4L) at room temperature. The mixture was heated to 145-150°C (internal temperature) and kept for ~3h. The reaction mixture was allowed to cool to 80°C and water (2.8L) was added. The reaction mixture was cooled to RT (25-28°C), and the precipitated solid was filtered and washed with water (1L). It was sucked dry, air dried for 12 h, and then vacuum dried at 40 °C for 5 h to provide 30.5 g of a mixture of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one and (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one. The aqueous portion was extracted with ethyl acetate (2 x 2 L). The combined extracts were washed with water (2 L), dried over sodium sulfate and concentrated to give a mixture of the product and ethylene glycol. It was stirred with water (500 mL) for 1 h, filtered and dried to yield an additional 3.5 g of (±)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one. The crude product was 97.84% pure by LCMS analysis. Chiral HPLC analysis showed a ratio of 53:47 of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one:(+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0730] Alternative procedure for the chiral resolution of 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0731] 68 g of (±)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one were submitted to SFC purification using the conditions described below; 27 g of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one were obtained. )-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one and 32 g of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one. 32 g of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one recovered from this batch were racemized for two or more cycles. At the end of three racemization cycles and three SFC purifications, 44.9 g of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one, 9 g of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one were obtained. yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one and 4 g of (±)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0732] Preparative SFC conditions

[0733]

[0734] The conformational interconversion of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one in NMP.

[0735] (+)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (250.2 mg, 0.49 mmol) was dissolved in NMP (24 mL, Alfa Aesar, J29Z048) at room temperature. The mixture was refluxed at an angle for more than 3 hours, with 100 μL aliquots periodically withdrawn by vacuum. Each aliquot was diluted with Chromasolv ethanol (900 μL) and purified by chiral HPLC (Agilent LC1100, Phenomenex Lux Cellulose-1, 4.6×100 mm, 5 μm 1.5 mL min -1 flow rate) and achiral HPLC (Waters LC 2695, Waters XBridge C18, 4.6 × 150 mm, 3.5 μm, 35 °C column temperature, 1.0 mL min -1 The results are Figure 2 Shown in.

[0736] Conformational interconversion of (+)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one in ethylene glycol.

[0737] (+)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one (250.2 mg, 0.49 mmol) was dispersed in ethylene glycol (25 mL, Beantown Chemical, 50009421) at room temperature. The mixture was tilted at reflux for more than 3 hours, and 100 μL aliquots were periodically withdrawn by vacuum. Each aliquot was diluted with Chromasolv ethanol (900 μL) and analyzed by chiral HPLC (Agilent LC 1100, Phenomenex Lux Cellulose-1, 4.6×100 mm, 5 μm 1.5 mL min -1 flow rate) and achiral HPLC (Waters LC 2695, Waters XBridge C18, 4.6 × 150 mm, 3.5 μm, 35 °C column temperature, 1.0 mL min -1 The results are Figure 3 Shown in.

[0738] Water-induced precipitation of 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one in ethylene glycol.

[0739] Ethylene glycol (12.0 mL, 9.8 mg mL) with pyridine (120 μL) was added. -1 ) in the solution of 3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one from T 内部 The mixture was cooled from 142°C to 90°C. Water was added in portions of approximately 1 mL to a total added volume of 9.02 mL (75.2% v / v based on the starting solution). No precipitation was observed during or after the addition of any water. The solution was allowed to cool to room temperature and aged for more than 96 hours. Crystallization began during the cooling process, with T nucleation ≈ 75°C. The mother liquor was sampled, filtered through a 0.45 μm filter and an aliquot (100 μL) was withdrawn, diluted with Chromasolv ethanol (900 μL) and purified by non-chiral HPLC (Waters LC 2695, Waters XBridge C18, 4.6×150 mm, 3.5 μm, 35°C column temperature, 1.0 mL min -1 The relative proportion of the precipitated product was calculated by comparing the ratio of the peak area (pyridine: product) in the sample to the completely dissolved starting material. 84.2% of the initially dissolved product was determined to have crystallized. Note that a small amount of additional crystallization was observed in the filtrate upon prolonged standing.

[0740] Chemical purity of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one

[0741] A summary of impurities and related substances observed in the drug substance batches is presented in Table 1. Batch 4, Batch 5, Batch 6, and Batch 7 are all clinical batches and did not have impurities or related substances at levels higher than those tested in toxicology studies. Impurities < 0.05% are not shown in the table.

[0742] There are some method differences between the methods used for batch 1 (toxicology) and the GMP batches. For batch 1, purity was assessed using an unvalidated laboratory UPLC method in development, and chiral purity testing used SFC (%, area). These SFC chirality and UPLC purity testing methods are typically used for early development batches. For GMP batches, impurities and related substances and chiral purity testing were validated using HPLC methods and appropriate methods. Unless otherwise stated, the relative retention times (RRTs) presented in Table 1 below are all from the HPLC method.

[0743] Table 1

[0744]

[0745] N / A = Not tested, reagent only in recovered batches

[0746] ND = Not Detected

[0747] LOQ = 0.03%

[0748] BRT = Below reporting threshold

[0749] * = Recycling batch manufacturing process B

[0750] ** = RRT from UPLC method

[0751] Residual solvent specifications were established according to ICHQ3C guidelines. A tabular summary of the observed levels of solvent levels in (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one is given in Table 2 below.

[0752] Table 2

[0753]

[0754]

[0755] N / A = Not tested, reagent only in recovered batches

[0756] ND = Not Detected

[0757] Inorganic Impurities: Potential inorganic impurities include heavy metals, palladium from coupling reaction catalysts used in chemical synthesis, and drying and filtering agents employed in the manufacturing process. Heavy metals are controlled during the manufacturing process using standard manufacturing equipment and heavy metal tests included in the drug substance specification. Palladium residues in the drug substance manufacturing process are controlled during multiple isolation procedures during the manufacturing process. The recommended palladium specification (not to exceed 50 ppm) is based on the recommended daily exposure for orally administered drug products in the USP chapter on elemental impurity limits. Drying and filtering agent residues are controlled by a subsequent filtration step to remove residues from reagents and heat tests included in the drug substance specification. The data presented in Table 3 confirm that all potential inorganic impurities are well controlled and below the specified limits.

[0758] Table 3

[0759]

[0760] ND = Not Detected

[0761] BLQ = Below Level of Quantification

[0762] Compound No. 65, Example B: Preparation of 3-chloro-4-((5-fluoropyrimidin-4-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one.

[0763]

[0764] The title compound was prepared according to Compound 49, Example A through Step E, except alkylating 3-chloro-4-hydroxy-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one with 4-(chloromethyl)-5-fluoropyrimidine instead of 2-(chloromethyl)-3,5-difluoropyridine to produce the desired benzyl ether. The title compound was prepared according to the general procedure of Compound 49, Example A, Step F.

[0765] Example 2: Single crystal structure determination

[0766] Sample preparation for X-ray crystal structure determination of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one.

[0767] In a 10 mL scintillation vial, 100 mg of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one (6 batches, GVK Bio, Hyderabad, India) was dissolved in 1 mL of gently warmed anhydrous acetonitrile (Sigma-Aldrich). The vial was sealed and the cap loosely screwed on. X-ray quality crystals were obtained in about four days and then shipped to the University of Toledo Instrument Center for data collection.

[0768] The X-ray structure of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one was determined.

[0769] Crystals of suitable size were obtained by slow evaporation of acetonitrile at room temperature. Single needle-shaped crystals were mounted on MiTeGen TM The cryo-loop was used with the central axis of the needle offset from the axis of rotation. Preliminary analysis and data collection was performed at 100 K using copper Kα radiation. and equipped with IμS Cu source and Oxford Cryostream TM Bruker-Nonius Kappa APEX II Duo for cryogenic equipment TM Diffractometer performed.

[0770] The total exposure time for data collection was 67.77 hours. Frames were integrated using the narrow frame algorithm with the Bruker SAINT software package (reference: Bruker Analytical X-Ray, Madison, WI, 2015). Integration of the data using the triclinic unit cell yielded a total of 11,397 reflections with a maximum θ angle of 66.98° ( resolution), of which 11397 are independent (average redundancy 1.000, completeness = 99.4%, Rint = 4.58%, Rsig = 4.38%) and 9621 (84.42%) are greater than 2σ(F2). The final unit cell constant ( α=79.27(3)°, β=81.35(3)°, γ=69.05(3)°, ) XYZ centroid correction based on 8394 reflections above 20σ(I) where 10.26°<2θ<136.1°. The data were corrected for absorption effects using a multi-scan method (SADABS) (reference: Bruker Analytical X-Ray, Madison, WI, 2014). The minimum to maximum apparent transmittance was 0.881.

[0771] The structure was determined and modified using the Bruker SHELXTL software package (reference: Sheldrick, GM (2008). Acta Cryst. A64, 112-122) using space group P1, where Z = 2 for the formula units, C25.25H22.38ClF2N5.12O3. The final anisotropic full matrix least squares correction of F2 had 836 variables, the observed data converged to R1 = 4.90%, and all data converged to wR2 = 13.21%. The goodness of fit was 1.048. The maximum peak of the final difference electron density synthesis was And the maximum hole is have Based on the final model, the density was calculated to be 1.401 g / cm3 and F(000), 538 e-.

[0772] The unit cell contains two crystallographically independent molecules C 25 H 22 N5O3F2Cl and 0.25 acetonitrile molecules. All crystals evaluated were twinned by rotating 180° around the a-axis. The crystals showed a twinning ratio of 0.75:0.25. Both C 25 H 22 The N5O3F2Cl molecule shows identical rotamers. However, one of them is slightly disordered in the cyclo-C(CH3)2(OH) region. The ratio of the two different orientations is about 85:15. A smaller fraction of OH-hydrogen atoms is not included in the correction. By minimizing w(Fo 2 -Fc 2 ) 2 To carry out full matrix least square correction.Non-hydrogen atoms are anisotropically corrected to converge.Fully occupied hydrogen atoms are located and corrected from difference Fourier synthesis, and the hydrogen atoms on disordered part and acetonitrile molecule are calculated on idealized position and processed using appropriate riding model and atomic displacement parameters constrained to bonded atoms.Final residual value and structure correction parameters are listed in Table 4 and Table 5.

[0773] The positions and isotropic displacement coefficients of hydrogen atoms and a complete list of anisotropic displacement coefficients of non-hydrogen atoms are listed in Tables 6-10.

[0774] Table 4. Sample and crystal data of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one

[0775]

[0776] Table 5. Data collection and structure refinement of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0777]

[0778] Table 6. (-)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-

[0779] Atomic coordinates and equivalent isotropic atomic displacement parameters of dimethyl-2H-[1,4'-bipyridyl]-2-one

[0780] U(eq) is defined as the orthogonalized U ij One third of the tensor's trajectory.

[0781]

[0782]

[0783]

[0784]

[0785] Table 7. Bond lengths of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0786]

[0787]

[0788]

[0789] Table 8. Bond angles (°) of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridinyl]-2-one

[0790]

[0791]

[0792]

[0793]

[0794] Table 9. Anisotropic atomic displacement parameters of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0795] The anisotropic atomic displacement factor exponent takes the following form: -2π 2 [h 2 a *2 U 11 +...+2hka * b * U 12 ]

[0796]

[0797]

[0798]

[0799] Table 10. Hydrogen atom coordinates and isotropic atomic displacement parameters of (-)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one

[0800]

[0801]

[0802] Example 3: Solubility Evaluation of Compound 49a

[0803] The solubility of compound 49a in 12 solvents was evaluated to support the solvent selection for subsequent crystal form screening experiments. At room temperature (RT; ~ 23 ° C), the solubility was visually estimated by adding a small aliquot of solvent to a fixed amount of solid (~ 10 mg) until the dissolution point or maximum volume of 1.8 mL was reached. The sample containing undissolved solid was heated to 40 ° C for 1 hour at room temperature, and the dissolution was visually evaluated. Compound 49a showed high solubility (> 100 mg / mL) in DCM, DMSO and THF; moderate solubility in MeCN, MeOH, EtOAc and IPA: water (9: 1); and low solubility (< 10 mg / mL) in five other solvents evaluated at room temperature. The results are shown in Table 11.

[0804] Table 11 - Estimated solubility of compound 49a in 12 solvents at RT and 40°C

[0805]

[0806] Example 4: Screening of crystal forms of compound 49a

[0807] The crystal form screening study involved a total of 48 pure and binary solvent systems that addressed the intermediate solubility of the input materials and provided a diverse set of polarity, dielectric constant, dipole moment, and hydrogen bond donor / acceptor properties. w ) 1 The aqueous solvent was used to detect the formation of hydrates. The temperature range was between 40°C and -20°C.

[0808] Screening studies included the following crystallization modes:

[0809] API slurry is aged at 5-40℃ for 4 days (TC)

[0810] Rapidly cool the clear saturated solution from 40°C to -20°C and keep it at -20°C for three days (RC)

[0811] The clear solution was evaporated slowly at room temperature for 14 days.

[0812] After 14 days, the solvent was rapidly evaporated under reduced pressure from the solution that produced no solid during the slow evaporation.

[0813] (EV).

[0814] A summary of the results of the screening study is shown in Table 12.

[0815] Table 12 Results of crystal form screening

[0816]

[0817]

[0818] Example 5: Single crystal structure determination of compound 49a (Form A)

[0819] The crystalline form of compound 49a has been characterized using single crystal X-ray diffraction relative to the absolute stereochemical configuration of the spatial arrangement of atoms. A detailed description of the determination of the structure by X-ray diffraction is provided in Stout & Jensen, X-Ray Structure Determination: A Practical Guide, Macmillan Co., New York (1968), Chapter 3 (which is incorporated herein by reference). Alternatively, the unique spatial arrangement of atoms in three dimensions within the lattice can be characterized by X-ray powder diffraction analysis. Cullity, BD Elements of X-ray Diffraction. Addison–Wesley, (1978) ISBN 0-201-01174-3 Chapter 14 (which is incorporated herein by reference). XRPD data consists of experimentally determined 2θ position values, intensity values ​​of multiple crystallographic reflections (also referred to as Bragg reflections) and their peak shapes. XRPD data can be analyzed computationally, including by the method of Rietveld correction (Rietveld refinement). A detailed description of the Rietveld correction of X-ray powder diffraction data is provided in Pecharsky, Vitalij K.; Zavalij, Peter Y. (2009) Fundamentals of powder diffraction and structural characterization of materials (2nd ed.). New York: Springer. ISBN 978-0-387-09579-0. OCLC 314182615, which is incorporated herein by reference.

[0820] XRPD data can be collected at various temperatures or pressures to facilitate Rietveld correction. Experimental XRPD data including 2θ values, d-spacing, Bragg reflections, and intensity values ​​can be compared with simulated XRPD patterns derived from single crystal structure determinations representing idealized pure powders using calculation methods such as Macrae, Clare F., et al. "Mercury 4.0: from visualization to analysis, design and prediction." Journal of Applied Crystallography vol.53, 226-235.1Feb.2020, doi: 10.1107 / S1600576719014092.

[0821] It will be appreciated by those of ordinary skill in the art that it is possible to obtain an X-ray powder diffraction pattern with measurement errors that depend on the measurement conditions employed in data collection. It is generally accepted that the peak shapes, intensity values, and 2θ positions derived from an X-ray powder diffraction pattern may fluctuate depending on the type of instrument used, the measurement conditions, and the computational analysis methods performed. It should be further understood that the 2θ values ​​and their relative intensities may also vary, and therefore the true order of the intensity values ​​should not be considered.

[0822] In addition, the experimental error of the diffraction angle measurement of the conventional X-ray powder diffraction pattern is typically about 5% or less. When describing the position of the 2θ diffraction peak, the assessment of the degree of measurement error should be considered. Therefore, it should be understood that the crystalline form described in the present invention is not limited to providing a crystalline form of an X-ray powder diffraction pattern that is exactly the same as the X-ray powder diffraction pattern depicted in the accompanying drawings disclosed herein. Any crystalline form that provides an X-ray powder diffraction pattern that is substantially the same as those disclosed in the accompanying drawings falls within the scope of the present invention. The ability to determine that the X-ray diffraction pattern is substantially the same is within the capabilities of ordinary technicians in the art. Similarly, it should be understood that any crystalline form that provides differential scanning calorimetry (DSC) and / or thermogravimetric analysis (TGA) that is substantially the same as those disclosed in the accompanying drawings falls within the scope of the present invention. The ability to determine that these patterns are substantially the same is within the capabilities of ordinary technicians in the art.

[0823] Crystalline Form A of compound 49a is anhydrous and is obtained from the crystallization conditions described in Example 4 using various organic solvents and organic / aqueous solvent systems.

[0824] X-ray powder diffraction (XRPD) diffractograms were obtained on a PANalytical X'Pert Pro diffractometer and X'celerator using Ni-filtered Cu Ka (45 kV / 40 mA) radiation and a step size of 0.02° 2θ.TM Acquired on a RTMS (Real Time Multiple Stripe) detector. Configuration on the incident beam side: fixed divergence slit (0.25°), 0.04rad Soller slit, anti-scatter slit (0.25°) and 10mm beam mask. Configuration on the diffracted beam side: fixed divergence slit (0.25°) and 0.04rad Soller slit. The sample was placed flat on a zero-background silicon wafer.

[0825] With respect to the results of simulated XRPD data from crystalline Form A of Compound 49a, the values ​​of the prominent Bragg reflections, their 2θ positions and d-spacing values ​​are shown in Table 13 and the XRPD patterns are shown in Figure 4 Shown in.

[0826] Table 13: Experimental and simulated XRPD data

[0827]

[0828]

[0829] Differential scanning calorimetry (DSC) was performed with a TA Instruments Q100 differential scanning calorimeter equipped with an autosampler and a refrigerated cooling system under a 40 mL / min N2 purge.DSC thermograms were obtained at 15°C / min in a crimped Al pan.

[0830] Thermogravimetric analysis (TGA) thermograms were obtained with a TA Instruments Q500 thermogravimetric analyzer at 15°C / min in a Pt or Al pan under a 40 mL / min N2 purge.

[0831] DSC analysis showed that crystalline Form A of compound 49a exhibited a melting / racemization event at 187.92°C, followed by a recrystallization event at 195.8°C, and finally a sharp endotherm at 253.5°C (racemate melting). Negligible weight loss (0.7%) was observed by TGA between 25-256°C. The DSC and TGA thermograms were Figure 5 Shown in.

[0832] Fourier Transform Infrared Spectroscopy (FT-IR): The characteristic spectral absorbance data from FT-IR of crystalline Form A of Compound 49a showed the location of the significant IR active region and its functional group assignment is shown in Table 14. The FT-IR absorbance curve showing the location of the significant IR active region is Figure 6 Shown in.

[0833] Table 14. FT-IR absorbance data

[0834]

[0835]

[0836] Example 6: Expansion of the pure phase of compound 49a

[0837] A scale-up procedure has been developed to produce the phase pure crystalline Form A of compound 49a.

[0838] Compound 49a (4.927 g) was combined with MeOH / 5% water (40 mL) and the temperature was cycled between 40-5° C. with stirring for 4 days. An aliquot was sampled, separated by filtration, air dried for 30 minutes and analyzed by DSC, which showed the presence of the racemate.

[0839] About 0.5mL is transferred to a 2mL vial and heated to 60°C, filtered through a 0.2μm syringe filter, and the filtrate is cooled to 5°C. No solid is observed. Water (60μL) is added dropwise under stirring and solid is observed. Solid is separated by filtration, air-dried and analyzed by DSC, showing that no racemate exists. MeOH / 5% water (20mL) is added and the slurry is heated to 60°C and stirred for 30 minutes. The slurry is filtered into a clean bottle through a 0.2μm syringe filter, naturally cooled to room temperature, and water (6mL) is added in 500μL aliquots so that the filtrate remains turbid. The filtrate is inoculated and stirred at room temperature for 30 minutes. The slurry is sampled aliquots, filtered, air-dried for 15 minutes and analyzed by DSC, showing that no racemate exists. The slurry is separated by filtration using Whatman#1 paper, and the mother liquor is recovered to wash the slurry bottle and added to the filter. The filter cake was air dried for 1 hour and dried in a vacuum oven at 50° C. with slow N 2 venting for 16 hours. Yield: 3.210 g (65%).

[0840] DSC analysis showed that crystalline Form A of compound 49a exhibited a single melting event at 192.6°C. No racemate was observed by DSC analysis. Negligible weight loss (<0.1%) was observed by TGA between 25-200°C. DSC and TGA thermograms were Figure 7 Shown in.

[0841] Example 7: Multi-kilogram crystallization of compound 49a

[0842] A multi-kilogram scale crystallization procedure has been developed to produce crystalline Form A of compound 49a.

[0843] Compound 49a (1.0 equivalent) and isopropanol (19 volumes) were loaded into a reactor at 25-35°C. The reaction mass was then heated to 72-77°C and stirred to a uniform solution. The reaction mass was kept for 1-2 hours. The reaction mass was filtered at 72-77°C. The reaction mass was cooled to 55-58°C, and Form A (0.005T w / w) of compound 49a was added as a seed material. The temperature was raised to 70-75°C and stirred for 1-2h. The reaction mass temperature was adjusted to 57-60°C and stirred at 55-58°C for 3-4h. The reaction mass was cooled to 25-30°C. Stirred for 12-15h. The reaction mass was cooled to 7.5-12.5°C over a period of 2-3h, and stirred for 4-5h. The solid was filtered and washed with IPA (2 volumes). The material was dried at 50-55°C until the sample met the requirements of chiral purity, individual impurity content and total impurities by HPLC to obtain Form A of Compound 49a as an off-white solid. The dried sample was sent for XRPD and DSC analysis. The final product was sampled for determination of water content by Karl Fischer titration. The results of 3 batches are shown in Table 15.

[0844] Table 15

[0845]

[0846] Biological Assessment

[0847] List of Biological Assessment Abbreviations

[0848] p38 Class of mitogen-activated protein kinases that respond to stress stimuli

[0849] MAP mitogen-activated protein kinase

[0850] MK2 is also called MAPKAPK2. It refers to MAP kinase activated protein kinase 2

[0851] PRAK p38 regulatory / activating kinase

[0852] GST Glutathione S-transferase

[0853] Hsp27 Heat shock protein 27

[0854] BSA bovine serum albumin

[0855] DTT Dithiothreitol

[0856] ATP Adenosine triphosphate

[0857] IC 50 The amount of drug needed to inhibit half of the process

[0858] EC 50The concentration of a drug that induces a response halfway between baseline and maximum limit after a specified exposure time

[0859] TNF Tumor necrosis factor

[0860] IL interleukin

[0861] JNK c-Jun N-terminal kinase

[0862] RPMI Roswell Park Memorial Institute medium. Medium for cell and tissue culture

[0863] HWB Human Whole Blood

[0864] DMEM Dulbecco's Modified Eagle's Medium. Enriched with vitamins and nutrients for cell culture.

[0865] FBS Fetal Bovine Serum

[0866] RASF rheumatoid arthritis synovial fibroblasts

[0867] Tiny-TIM TNO gastrointestinal model of stomach and monocompartmental small intestine

[0868] HKW Strong Contraction (House Keeper Wave)

[0869] TMIS Test Material Information Sheet

[0870] BAmax Maximum Bioavailability

[0871] Tmax The time interval for recording BAmax

[0872] Example 8: Release and bioaccessibility of Form A of compound 49a from an oral dosage form during transport through the dynamic gastrointestinal tract model tiny-TIM

[0873] A study was conducted to compare the release, solubility and absorption availability (bioaccessibility) of a formulated 50 mg strength tablet (composed of 12.5 wt% crystalline Form A of compound 49a, 71 wt% silicified microcrystalline cellulose, 10 wt% mannitol, 5 wt% crospovidone, 0.75 wt% hydrophilic fumed silica and 0.75 wt% magnesium stearate) during transit through a dynamic computer-controlled model of the stomach and small intestine (tiny-TIM) under fasting and fed state conditions. The findings from these experiments helped characterize the bioaccessibility profile of the formulation under both conditions.

[0874] Test system: Studies were performed in a dynamic, multicompartmental in vitro system of TNO in the stomach and small intestine (tiny-TIM). The tiny-TIM system consists of a stomach compartment and a small intestinal compartment ( Figure 8 ). The chamber consists of two glass units with flexible silicone inner walls surrounding the luminal material. The space between the inner and outer walls is filled with water. Peristaltic mixing of chyme is the result of alternating compression and relaxation of the flexible inner wall. The chambers are connected by peristaltic valve pumps, which are opened and closed in sequence, allowing chyme to be transported through the chambers over time. In this way, oral dosage forms / APIs are exposed to local changes and physiologically relevant conditions in the stomach and small intestine of the tiny-TIM. The tiny-TIM system simulates the gastrointestinal transport of intraluminal pH, enzyme activity, bile salt concentration, peristaltic motion, and contents. The set points for gastrointestinal simulation are controlled and monitored by a specific computer program. The released and dissolved drug molecules are removed from the intestinal lumen through the semipermeable membrane unit connected to the small intestinal chamber. This allows the evaluation of the so-called bioaccessible fraction, i.e., the fraction of the drug available for absorption in the small intestine.

[0875] In order to simulate fasting state conditions, a cup of water (240mL) was applied to the tiny-TIM system. In order to simulate fed state conditions, a high-fat meal (HFM) was used for clinical research according to the FDA's recommendations. The meal contained approximately 50% of energy in fat, 20% of energy in protein, and 30% of energy in carbohydrate form. The meal consisted of eggs, bacon, toast, potatoes, milk, butter, and margarine. The meal was prepared in batches, divided into 150-gram portions and stored at ≤-18°C. Each time tiny-TIM was run, a meal was used.

[0876] Experiments in tiny-TIM are carried out under average physiological conditions in the gastrointestinal tract described for humans in fasting and feeding states. These conditions especially include dynamics of gastric emptying and pH decline, intestinal transit time, strong contraction (House Keeper Wave), gastric and intestinal pH values ​​(Table 16) and the composition and activity of secretory products. Digested and soluble (low molecular) compounds are continuously removed from the intestinal compartment of the model via a special membrane system. Before each experiment is carried out, fresh secretion fluid (e.g., gastric juice containing enzymes, electrolytes, bile and pancreatic juice) is prepared, pH electrodes are calibrated, and semipermeable membrane (hollow fiber) units are installed. By transferring residual material from the gastric compartment to the intestinal compartment, strong contraction (HKW) is simulated after 60 minutes (fasting state) and 180 minutes (fed state).

[0877] Table 16 Parameters describing average gastrointestinal physiological conditions (HFM = high fat meal) of healthy young adults simulated in tiny-TIM

[0878] Tiny-TIM Feeding state (HFM) Fasting state <![CDATA[ Gastric atrioventricular tiny-TIM ]]> Intake (total) 300g 270g Meals (HFM) 150g - Water and artificial saliva 140g 240g Gastric starting fluid 10g 30g Gastric emptying T1 / 2 80min 20min Strong contraction 180min 60min Gastric pH 6.5 to 1.7 in 180 min 3.0 to 1.8 in 30 min <![CDATA[ Tiny-TIM ]]> pH intestinal compartment 6.5 6.5 Duration of the experiment 6 hours 5 hours

[0879] Through a semipermeable membrane unit (Fresenius The release of the drug molecules from the intestinal cavity (P1dry) and the filtration of dissolved / solubilized drug molecules allow the assessment of the so-called bioaccessible fraction, i.e., the fraction of the drug available for absorption in the small intestine. The analysis of these samples produces bioaccessibility characteristics over time. The filtrate fraction of the intestinal compartment is collected at intervals of 30 minutes (0-30min, 30-60min, 60-90min, 90-120min, 120-150min, 150-180min, 180-210min, 210-240min, 240-270min, 270-300min, 300-330min and 330-360min). The volume collected in each time period is measured and a subsample is taken out, immediately diluted in 50% acetonitrile in Milli-q water and stored in the dark at 2-10°C until analysis.

[0880] Lumen samples from the intestinal compartment were collected to determine the concentration of crystalline Form A of Compound 49a. The samples were collected every 30 minutes (30 min, 60 min, 90 min, 120 min, 150 min, 180 min, 210 min, 240 min, 270 min, 300 min, 330 min, 450 min, 500 min, 600 min, 700 min, 800 min, 900 min, 1000 min, 1500 min, 1800 min, 2100 min, 2400 min, 2700 min, 3000 min, 3300 min, 450 min, 5000 min, 8000 min, 9000 min, 15000 min, 18000 min, 21000 min, 24000 min, 2700 1 min and 360 1 The samples were collected every 30 minutes (30min, 60min, 90min, 120min, 150min, 180min, 210min, 240min, 270min, 300min, 330min and 360min).

[0881] After the experiment was completed, the remnants of the gastric and intestinal compartments were collected separately. Any remnants of the dosage form were also collected separately. Each compartment was rinsed twice with 50% acetonitrile in Milli-q water. This rinse was combined with the remnant sample of the same compartment, the volume was measured and stored at 2-10°C in the dark until analysis.

[0882] The absolute amount of API recovered in the sample was calculated by multiplying the concentration analyzed in the sample by the volume collected (Equation 1).

[0883] A(mg)=C 样品 (μg / ml)·10 -3 ·V 样品 (mL)(1)

[0884] The recovery of the API was determined by the sum of all amounts recovered in the filtrate fractions of the jejunum and ileum, the ileal effluent and the residual and flushing fractions of the gastric, duodenal, jejunal and ileal compartments and the drug product. The total recovery was expressed as a percentage of the added amount (Equation 2).

[0885]

[0886] Bioaccessibility (% ingested) was calculated by expressing the amount of API recovered from the filtrate as % ingested (Equation 3).

[0887]

[0888] The results of replicate runs are presented as mean ± SD. For SD, the STDEVP function was used in Microsoft Excel (Equation 4).

[0889]

[0890] The test product (TP, crystalline Form A of compound 49a) was tested at 50 mg in duplicate under fasting and fed conditions to identify possible food effects. The individual values ​​expressed as a percentage of intake (% of intake) are presented in Tables 17 and 18. For all four tiny-TIM runs performed in Phase 2, the recovery ranged from 97.8%-103.7% of intake (Tables 17 and 18).

[0891] Table 17

[0892]

[0893]

[0894] Table 18

[0895]

[0896] To correct for differences in recoveries, the results described below are expressed as percentage of recovery unless otherwise stated.

[0897] Mean small intestinal (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one bioaccessibility was 84.0±0.7% of recovery under fasting state conditions and 95.4±0.5% of recovery under fed state conditions (Table 19). The increased bioaccessibility of crystalline Form A of compound 49a under fed state conditions suggests a small positive food effect. The individual results of these experiments are expressed as a percentage of intake (% Intake) and the luminal concentrations in the intestinal compartment are presented in Tables 17 and 18. The values ​​expressed as a percentage of recovery (% Recovery) for each test product are found in Fig. 9 and Fig.10 middle.

[0898] Table 19: Total (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0899] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one, cavity-like

[0900] Samples, residue values ​​(% of recovery) and recovery (% of uptake) (presented as mean ± standard deviation (stdevp))

[0901]

[0902] The highest levels of bioaccessibility (BAmax) of (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one were observed in samples collected between 60-90 min in the fasting state and between 90-120 min in the fed state conditions ( Fig.10 and Table 20). Average T max Each BA at the time interval max The values ​​are as follows: 18.7% and 19.7% of the recovery for the fasted state run, and 18.9% and 19.1% of the recovery for the fed state run. This means that although T max shifted, but in both fasting and fed conditions, BA max The values ​​remain similar.

[0903] Table 20: T for stage 2 tiny-TIM operation max and BA max (For BA maxValues ​​are presented as mean ± standard deviation)

[0904]

[0905] To gain insight into intestinal concentrations of (P)-3-chloro-4-((3,5-difluoropyridin-2-yl)methoxy)-2'-(2-(2-hydroxypropan-2-yl)pyrimidin-4-yl)-5',6-dimethyl-2H-[1,4'-bipyridyl]-2-one during the tiny-TIM experiment, luminal samples from the intestinal compartment were collected at different time points as indicated above. The results of these samples, which provide rapid insight into total luminal API concentrations, are presented in Fig.11 As expected, the total luminal concentration in the fasting state was highest within the first 60 minutes of the experiment, which corresponds to a gastric emptying half-life of 20 minutes in the fasting state. For the fed state, the gastric emptying half-life is 80 minutes, so a luminal concentration curve corresponding to slower gastric emptying under fed state conditions was generated.

[0906] Example 9: p38 Inhibitory Potency and p38 / MK2 Substrate Selectivity

[0907] This study evaluates the effectiveness of the compounds of the present invention in inhibiting p38 pathways. p38 activates MK2 and PRAK via phosphorylation, and then both MK2 and PRAK interact with Hsp27, thereby causing increased inflammation and reduced ability to handle shock. This study measures the amount of the compounds of the present invention necessary to inhibit the activation of MK2 and PRAK by half. This is a measurement of the effectiveness of the compounds of the present invention in helping to reduce inflammatory responses, which helps treat many diseases (including autoimmune conditions, lymphomas, and rheumatoid arthritis). The novel MK2 substrate selective inhibition mechanism of the compound is evaluated in an enzyme assay, and the effectiveness of the inhibitor in blocking p38 / MK2 is compared with the effectiveness of the p38 / PRAK-induced phosphorylation of a peptide substrate derived from HSP-27. The ability of the p38α / MK2 and p38α / PRAK cascades to determine the form of the evaluation compound to inhibit the activated phosphorylated p38α. The kinase activity of p38α is determined by the ability of p38α phosphorylation GST-MK2 or GST-PRAK. The activation of MK2 or PRAK by p38α was quantified by measuring the phosphorylation of fluorescently labeled MK2 / PRAK specific peptide substrate Hsp27 peptide (FITC-KKKALSRQLSVAA). Phosphorylation of Hsp27 peptide was quantified using IMAP technology (Molecular Devices, Sunnyvale, California). Kinase reaction was carried out in 20mM HEPES pH 7.5, 10mM MgCl2, 0.01% Triton X-100, 0.01% BSA, 1mM DTT and 2% DMSO in 384-well plates (Greiner, 781280). Inhibitor concentration varied between 0.02-30000nM, while Hsp27 peptide substrate and MgATP were kept constant at 1 μM and 10 μM, respectively. Activated p38α was added to a final concentration of 30pM for reaction with non-phosphorylated 1nM GST-MK2 in a cascade reaction. For the p38α / PRAK cascade, inactivated GST-PRAK was kept constant at 10 nM, while p38α was added to a final concentration of 200 pM. The kinase reactions were incubated at room temperature and quenched after 120 min by the addition of IMAP binding solution. Under these conditions, approximately 20% of the substrate Hsp27 peptide was phosphorylated. Reactions were initiated by the addition of activated p38α, except for preincubation experiments, in which reactions were initiated by the addition of Hsp27 peptide and MgATP. Preincubations of p38α with inhibitors or p38α with inactivated GST-MK2 or inactivated GST-PRAK with inhibitors were performed at room temperature for 240 min at 2× final assay concentrations before the addition of ATP and Hsp27 peptide to initiate catalysis. The dose response IC 50p38α compound inhibitory potency was quantified using Ki values ​​or Ki values ​​from the p38α / MK2 cascade assay, whereas substrate selectivity was calculated as p38α / PRAK IC 50 Value: p38α / MK2IC 50 The compounds described herein evaluated in this assay are expected to provide therapeutic benefit in the treatment of p38 MAP kinase mediated diseases such as autoimmune diseases and lymphomas.

[0908] Compounds were tested according to the assay described above, generating the IC values ​​described below. 50 value.

[0909]

[0910]

[0911] Example 10: Regulation of cytokines in human monocytes

[0912] The p38 pathway has been shown to be essential for the biosynthesis of many proinflammatory cytokines, including TNFa, IL-1β, and IL-6. Therefore, inhibition of the p38 MAP kinase pathway will reduce inflammatory responses by reducing the biosynthesis of proinflammatory cytokines. This study shows the amount of the compound of the present invention required to inhibit the biosynthesis of TNFα, IL-6, and IL-1β (proinflammatory cytokines) by half. This reflects the role of the compounds of the present invention in helping to reduce inflammation, which helps treat many diseases, including autoimmune conditions, lymphomas, and rheumatoid arthritis. The efficacy and effectiveness of p38 inhibitors in blocking cytokine production were evaluated using the human U937 cell line. The U937 human promonocyte cell line was obtained from the American Type Culture Collection (Rockville, MD). These cells differentiate into a monocyte / macrophage phenotype as described by Burnette (Burnette et al, (2009). SD0006: a potent, selective and orally available inhibitor of p38 MAP Kinase, Pharmacology 84 (1): 42-60). Differentiated U937 cells (human peripheral blood mononuclear cells (hPBMC)) were seeded into 96-well tissue culture plates (200,000 cells / well) in complete medium. After 24 hours, cells were pretreated for 60 minutes in the presence or absence of the compound and then stimulated with LPS (0.1 μg / mL) for 4 hours. The culture medium was then collected for determination of TNFα, IL-6 or IL-1β levels by ELISA. Cytokine concentrations were extrapolated from the recombinant protein standard curve using a four-parameter logistic model and the IC was solved after iteration to the best least squares fit. 50 The compounds described above evaluated in this assay are expected to provide therapeutic benefit in the treatment of p38 MAP kinase mediated diseases such as lymphoma or inflammation.

[0913] Compounds were tested according to the assay described above, generating the IC values ​​described below. 50 value:

[0914]

[0915]

[0916] Example 11: Phosphoprotein Analysis in Human Monocytes

[0917] This study shows the effectiveness and selectivity of the compounds of the present invention in inhibiting the JNK pathway. The JNK pathway causes increased inflammation by promoting the production of inflammatory cytokines. The inhibition of this pathway will lead to reduced inflammation, and therefore will treat many diseases, including autoimmune diseases, lymphomas, and rheumatoid arthritis. Classical p38 inhibitors block the phosphorylation of downstream substrates of p38, while increasing the activity of parallel pathways (such as JNK). Phospho-HSP27 and phospho-JNK were used to evaluate the effects of different classes of p38 inhibitors on p38 and JNK pathway regulation in these two pathways. The efficacy and effect of p38 inhibitors on phosphoprotein levels were evaluated using the human U937 cell line. The U937 human promonocyte cell line was obtained from the American Type Culture Collection (Rockville, MD). These cells were differentiated into a monocyte / macrophage phenotype as described by Burnette (Burnette et al, (2009). SD0006: a potent, selective and orally available inhibitor of p38 MAP kinase, Pharmacology 84(1): 42-60). Suspension cells (in T75cm 2 The cells were grown in RPMI containing 10% fetal bovine serum (FBS) and antibiotics (about 500,000 per ml in tissue culture bottles). On the first day, phorbol 12-myristate 13-acetate (PMA, 20 ng / mL) was added to the culture bottle, and the cells were incubated overnight at 37°C / 5% CO2. The cells were washed by centrifugation on the second day and resuspended in fresh culture medium without PMA. On the third day, adherent cells were harvested by scraping, centrifuging and resuspending in fresh culture medium at a density of 1 million per ml. The U937 cells differentiated by PMA were then distributed to each well of a 96-well flat-bottom tissue culture plate (100 mL / well), and 100,000 cells / well were allowed to recover, incubate, and spend the night. On the day of the assay, fresh culture medium (50 mL / well) was added to the plate, followed by compound (25 mL / well, concentration response) for 1 hour. In the final assay volume of 100 mL, cells were stimulated with LPS (100 ng / mL). After 30 minutes, complete lysis buffer (50 mL / well MSD Tris lysis buffer supplemented with protease inhibitors and phosphatase inhibitors) was added and the plate was placed on a shaker at 4° C. for 30 minutes before freezing and storing at −20° C. Cell lysates (25 mL / well) were thawed and transferred from the assay plate to the Meso Scale detection plate for determination of phospho-Hsp27 / total Hsp27 or phospho-JNK / total JNK.

[0918] Compounds were tested according to the assay described above, generating the IC values ​​described below.50 and EC 50 value:

[0919]

[0920]

[0921] Example 12: Endotoxin-induced cytokine production from human whole blood: Human whole blood (HWB; 25-45 mL) was collected from NSAID-free donors into a vacuum blood collection tube containing sodium heparin (10 mL, 158 USP units), combined and gently shaken, and then distributed into each well of a 96-well round-bottom tissue culture plate (180 mL / well). Compounds (10 mL / well, concentration response) were added and gently mixed for 15-20 seconds using a disposable 96 polypropylene needle tool, and the plate was then incubated at 37°C / 5% CO2 for 1 hour. HWB were stimulated with LPS (100 ng / mL) in a final assay volume of 200 mL. After 3 hours, the plate was spun at 240×g for 5 minutes to precipitate red blood cells. Plasma was carefully transferred to another 96-well round-bottom plate and diluted 2-fold with assay medium (DMEM containing 10% fetal bovine serum (FBS) and antibiotics). Finally, the diluted plasma (25 mL / well) was transferred to the Meso Scal assay plate for the determination of IL-1, IL-6 or TNFα.

[0922] Compounds were tested according to the assay described above, generating the IC values ​​described below. 50 and EC 50 value:

[0923]

[0924]

[0925] Example 13: Determination of IL-1-induced IL-6 production in A549 cells

[0926] Adherent A549 cells (each T75cm 2Tissue culture flasks (approximately 5 million) were grown in F-12K medium containing 10% fetal bovine serum (FBS) and antibiotics. The cells were trypsinized, washed and resuspended at a concentration of 300,000 per milliliter. A549 cells were then distributed to each well of a 96-well flat-bottom tissue culture plate (100 mL / well), and 30,000 cells / well were allowed to recover, incubate, and stay overnight. On the day of the assay, fresh medium (50 mL / well) was added to the plate, followed by compound addition (25 mL / well, concentration response) for 1 hour. In a final assay volume of 100 mL, cells were stimulated with LPS (100 ng / mL). After 3 hours, the culture medium (25 mL / well) was transferred from the assay plate to a Meso Scale custom-coated assay plate for determining IL-6 levels. The assay plate was incubated overnight at 4°C, and then a sulfo-labeled antibody mixture (25 mL / well) was added for 1 hour at room temperature with vigorous shaking. Reading buffer (150mL / well, MSD 4× reading buffer diluted 4 times with dH2O) was added and the plate was read using a Meso Scale Sector Imager 6000. After the detection plate was electrically stimulated, the co-reactants in the reading buffer enhanced the electrochemical reaction, resulting in the release of energy in the form of light. The signal was captured and quantified by an internal CCD camera. The viability of A549 cells was determined using the MTT assay. After the cells were incubated with LPS for 3 hours and the culture medium was collected, the assay plate was inverted and gently tapped to remove any remaining liquid. MTT (1 mg / mL solution prepared in assay culture medium) (100mL / well) was added and the plate was returned to a 37°C / 5% CO2 incubator for 3 hours. The plate was inverted again to remove any liquid and allowed to dry overnight. Isopropanol (100mL / well) was added to dissolve the resulting formazan crystals and the plate was read at 570nm / 650nm using a Molecular Devices SpectraMax spectrophotometer.

[0927] Example 14: IL-1β-induced prostaglandin production in rheumatoid arthritis synovial fibroblasts (RASFs)

[0928] Rheumatoid arthritis synovial fibroblasts (RASFs) were derived from the inflamed synovium of female RA patients undergoing total knee replacement. Synovial tissue was isolated from adjacent cartilage and dispersed into single cells using collagenase. The cells were expanded and stored. RASF cells were further cultured as described by Burnette (supra). RASF cells were seeded into 96-well tissue culture plates (5×10 4Cells / well). After 24 hours, the culture medium was replaced with fresh growth medium containing 1% FBS. Cells were treated with compounds or dimethyl sulfoxide (DMSO) vehicle control with a series of concentrations (30000-0.01nM) for 1 hour, then stimulated with 1ng / mL IL-1β (R&D Systems, Minneapolis, MN) at 37°C for 18-20 hours and conditioned medium collection. PGE2 levels in the culture medium were quantified by ELISA (Cayman Chemical, Ann Arbor, MI). The compounds described above evaluated in this assay are expected to provide therapeutic benefits in the treatment of p38MAP kinase-mediated diseases (such as lymphoma or rheumatoid arthritis).

[0929] Example 15: Substrate selectivity in HUVEC cells

[0930] When identifying compounds by the biochemical characterization step of selective inhibition of p38 / MK2, it is then placed in a cell-based assay to verify the translatability of the enzyme to the cell. These assays use human umbilical vein endothelial cells (HUVEC) to demonstrate the inhibition of Hsp27 phosphorylation (a biomarker of p38 / MK2 activation), while retaining the production of tissue factor (TF) connected to another downstream substrate MSK of p38. In a 96-well format, adherent HUVEC (5 generations or less) are treated with serially diluted compounds (including non-selective p38 inhibitors as reference or a solvent for control) for 1 hour. For Hsp27 phosphorylation, cells are subsequently stimulated with 500pg / mL IL-1β for 0.5 hour, the culture medium is removed, the cells are lysed, and phosphorylation-hsp27 in the lysate is quantitatively measured by enzyme-linked immunosorbent assay (ELISA) (Life Technologies, Carlsbad, CA). The procedure for TF release is a similar ELISA-based assay (American Diagnostica, Stanford, CT), except that IL-1β stimulates for 5 hours. TF Inhibits IC 50 :HSP27 phosphorylation inhibitor IC 50 The ratio of is defined as the substrate selectivity index in these cells. The compounds described above evaluated in this assay are expected to provide therapeutic benefit in the treatment of p38 MAP kinase mediated diseases such as lymphoma and autoinflammatory diseases.

[0931] Example 16: Regulation of Canine B Cell Growth

[0932] p38MAP kinase inhibitors have been shown to uniquely inhibit canine B cell proliferation and survival. This selective effect on canine B cells can be used in the therapeutic treatment of canine B cell lymphoma, a fatal disease affecting >40,000 companion animals in the United States. Quantification of the effect of p38 inhibitors on B cell growth is a cellular indicator of efficacy in B cell lymphoma. The compounds described above evaluated in this assay are expected to provide therapeutic benefit in the treatment of p38MAP kinase-mediated diseases such as lymphoma. These assays use beagle spleens obtained under a protocol approved by the Animal Care and Use Committee of Saint Louis University in collaboration with Seventh Wave Laboratories. Leukocytes are separated from spleen cells by Histopaque 1077 centrifugation. To assess the effect on proliferation, leukocytes are then cultured in 96-well plates for 48 hours in the presence of a solvent or a test compound. Cells were stimulated with LPS for TLR4 stimulation, Staphylococcus aureus B cell mitogens or Concanavalin-AT cell mitogens, and proliferation was quantified using BRDU combined with ELISA (Roche, Mannheim, Germany). For apoptosis experiments, leukocytes were plated on 96-well polypropylene U-bottom plates and treated with p38 MAP kinase inhibitors or staurosporine (as a positive control) in the absence or presence of actinomycin D or cycloheximide (if needed to increase the apoptosis rate) until 24 h. Apoptosis was determined using the Caspase-Glo 3 / 7 luminescent assay (Promega, Madison, WI). In both assays, the values ​​generated after incubation with increasing concentrations of inhibitors were compared to negative controls without inhibitors.

[0933] Example 17: LPS-induced TNFα production in rats

[0934] Rats were fasted for 18 hours before oral administration, and free drinking water was allowed throughout the experiment. Each treatment group consisted of five animals. The compound was prepared as a suspension in a solvent consisting of 0.5% methylcellulose (Sigma Aldrich, St.Louis, MO), 0.025% Tween 20 (Sigma Aldrich). Compounds or vehicles were administered by oral gavage with a volume of 1 mL. Two vehicle groups were used in each experiment to control the variability within the experiment. LPS (Escherichia coli serotype 0111: B4, Sigma Aldrich) was administered with a dose of 1 mg / kg in 0.5 mL sterile saline (Baxter Healthcare, Deerfield, IL) 4 hours after compound intravenous injection. Blood was collected in serum separation tubes via cardiac puncture 90 minutes after LPS injection (corresponding to the time point of maximum TNFα and IL-1β production). After solidification, serum was taken out and stored at -20°C, and IL-1β and TNFα levels were quantitatively measured by ELISA (Burnette the same). The compounds described above evaluated in this assay are expected to provide therapeutic benefit in the treatment of p38 MAP kinase mediated diseases such as lymphoma or inflammation.

[0935] Implementation methods of the disclosure

[0936] While preferred embodiments have been depicted and described herein in detail, it will be apparent to those skilled in the relevant arts that various modifications, additions, substitutions and the like are possible without departing from the spirit of the invention and are therefore considered to be within the scope of the invention as defined in the accompanying claims.

[0937] The present invention also provides the following non-limiting embodiments.

[0938] Embodiment 1 is a crystalline form of compound 49a:

[0939]

[0940] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0941] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0942] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0943] Compound 49a.

[0944] Embodiment 2 is the crystalline form of embodiment 1, wherein compound 49a is the free base.

[0945] Embodiment 3 is the crystalline form of any one of embodiments 1 and 2, wherein the crystalline form of compound 49a is Form A.

[0946] Embodiment 4 is the crystalline form of embodiment 3, wherein Form A is characterized by an XRPD pattern having a peak expressed at an angle 2θ of about 9.78 (± 0.2).

[0947] Embodiment 5 is the crystalline form of embodiment 3, wherein Form A is characterized by an XRPD pattern having peaks expressed at angles 2θ (± 0.2) at about 9.78 and about 15.51.

[0948] Embodiment 6 is the crystalline form of embodiment 3, wherein Form A is characterized by an XRPD pattern having peaks expressed as angles 2θ (± 0.2) at about 9.78, about 15.51, about 19.6, and about 25.92.

[0949] Embodiment 7 is the crystalline form of embodiment 3, wherein Form A is characterized by an XRPD pattern having peaks expressed as angles 2θ (± 0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.48.

[0950] Embodiment 8 is the crystalline form of embodiment 3, wherein Form A is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C and a final sharp endothermic melting event at about 254°C.

[0951] Embodiment 9 is the crystalline form of any one of embodiments 1-8, wherein the crystalline form further comprises no more than about 20 mol % of the corresponding M isomer of compound 49a.

[0952] Embodiment 10 is the crystalline form of any one of embodiments 1-8, wherein the crystalline form further comprises no more than about 0.25 mol % of the corresponding M isomer of compound 49a.

[0953] Embodiment 11 is the crystalline form of any one of embodiments 1-8, wherein the crystalline form is substantially free of the corresponding M isomer of compound 49a.

[0954] Embodiment 12 is the crystalline form of any one of embodiments 1-11, wherein compound 49a has a chemical purity of about 95% or greater.

[0955] Embodiment 13 is the crystalline form of any one of embodiments 1-12, wherein the crystalline form of compound 49a contains no more than about 20 mol % of other solid forms.

[0956] Embodiment 14 is the crystalline form of any one of embodiments 1-12, wherein the crystalline form of compound 49a contains no more than about 0.25 mol % of other solid forms.

[0957] Embodiment 15 is the crystalline form of any one of embodiments 1-12, wherein the crystalline form of compound 49a is substantially free of other solid forms.

[0958] Embodiment 16 is a pharmaceutical composition comprising a crystalline form of compound 49a:

[0959]

[0960] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0961] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0962] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0963] Compound 49a,

[0964] and pharmaceutically acceptable excipients.

[0965] Embodiment 17 is a pharmaceutical composition, which includes compound 49a, or a pharmaceutically acceptable salt thereof or a free base thereof, and compound 49b, or a pharmaceutically acceptable salt thereof or a free base thereof:

[0966]

[0967] (P)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0968] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0969] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0970] Compound 49a,

[0971]

[0972] (M)-3-Chloro-4-((3,5-difluoropyridin-2-yl)methoxy

[0973] yl)-2'-(2-(2-hydroxypropane-2-yl)pyrimidin-4-yl)-5',6-

[0974] Dimethyl-2H-[1,4'-bipyridyl]-2-one

[0975] Compound 49b,

[0976] wherein the molar ratio of compound 49a, or a pharmaceutically acceptable salt thereof, or a free base thereof, to compound 49b, or a pharmaceutically acceptable salt thereof, or a free base thereof, is about 4:1;

[0977] and pharmaceutically acceptable excipients.

[0978] Embodiment 18 is a pharmaceutical composition as described in any one of embodiments 16 and 17, wherein compound 49a is a free base.

[0979] Embodiment 19 is the pharmaceutical composition of any one of embodiments 16-18, wherein the crystalline form of Compound 49a is Form A.

[0980] Embodiment 20 is the pharmaceutical composition of embodiment 19, wherein Form A is characterized by an XRPD pattern having a peak expressed at an angle 2θ of about 9.78 (±0.2).

[0981] Embodiment 21 is the pharmaceutical composition of embodiment 19, wherein Form A is characterized by an XRPD pattern having peaks expressed at angles 2θ (±0.2) at about 9.78 and about 15.51.

[0982] Embodiment 22 is the pharmaceutical composition of embodiment 19, wherein Form A is characterized by an XRPD pattern having peaks expressed as angles 2θ (±0.2) at about 9.78, about 15.51, about 19.6, and about 25.92.

[0983] Embodiment 23 is the pharmaceutical composition of embodiment 19, wherein Form A is characterized by an XRPD pattern having peaks represented by angles 2θ (± 0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.48.

[0984] Embodiment 24 is the pharmaceutical composition of embodiment 19, wherein Form A is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C, and a final sharp endothermic melting event at about 254°C.

[0985] Embodiment 25 is the pharmaceutical composition of any one of embodiments 16-24, wherein the pharmaceutical composition further comprises no more than about 20 mol % of the corresponding M isomer of compound 49a.

[0986] Embodiment 26 is the pharmaceutical composition of any one of embodiments 16-24, wherein the pharmaceutical composition further comprises no more than about 0.25 mol % of the corresponding M isomer of compound 49a.

[0987] Embodiment 27 is the pharmaceutical composition of any one of embodiments 16-24, wherein the pharmaceutical composition is substantially free of the corresponding M isomer of compound 49a.

[0988] Embodiment 28 is the pharmaceutical composition of any one of embodiments 16-27, wherein compound 49a has a chemical purity of about 95% or greater.

[0989] Embodiment 29 is a pharmaceutical composition of any one of embodiments 16-28, wherein the crystalline form of compound 49a contains no more than about 20 mol% of other solid forms.

[0990] Embodiment 30 is a pharmaceutical composition of any one of embodiments 16-28, wherein the crystalline form of compound 49a contains no more than about 0.25 mol % of other solid forms.

[0991] Embodiment 31 is a pharmaceutical composition of any one of embodiments 16-28, wherein the crystalline form of Compound 49a is substantially free of other solid forms.

[0992] Embodiment 32 is the pharmaceutical composition of any one of embodiments 16-31, wherein the therapeutically effective amount is from about 10 mg to about 300 mg.

[0993] Embodiment 33 is the pharmaceutical composition of embodiment 32, wherein the therapeutically effective amount is about 50 mg.

[0994] Embodiment 34 is the pharmaceutical composition of any one of embodiments 16-31, wherein the pharmaceutical composition is an oral pharmaceutical composition.

[0995] Embodiment 35 is a tablet comprising the pharmaceutical composition of any one of embodiments 1-32.

Claims

1. Use of a crystalline form of compound 49a in the manufacture of a medicament for treating a condition:

2. The use as claimed in claim 1, wherein compound 49a is a free base.

3. The use according to claim 1, wherein the crystalline form of compound 49a is Form A.

4. The use of claim 3, wherein Form A is characterized by an XRPD pattern having a peak represented by an angle 2θ (±0.2) at about 9.

78.

5. The use of claim 3, wherein Form A is characterized by an XRPD pattern having peaks expressed at angles 2θ (±0.2) at about 9.78 and about 15.

51.

6. The use of claim 3, wherein Form A is characterized by an XRPD pattern having peaks expressed at angles 2θ (±0.2) at about 9.78, about 15.51, about 19.6, and about 25.

92.

7. The use of claim 3, wherein Form A is characterized by an XRPD pattern having peaks represented by angles 2θ (±0.2) at about 9.78, about 15.34, about 15.51, about 19.6, about 20.57, about 21.01, about 25.92, about 29.05, and about 29.

48.

8. The use of claim 1, wherein Form A is characterized by a DSC curve comprising an initial endothermic melting event with an onset temperature of about 188°C, followed by an exothermic recrystallization event at about 196°C, and a final sharp endothermic melting event at about 254°C.

9. The use of claim 1, wherein Form A is characterized by a DSC curve comprising a single melting event at about 192.6°C.

10. The use of claim 1, wherein Form A is characterized by an FT-IR spectrum comprising a peak at about 3486 cm -1 、3072cm -1 、2982cm -1 、1656cm -1 、1605cm -1 、1592cm -1 、1571cm -1 、1546cm -1 、1525cm -1 、1476cm -1 、1457cm -1 、1429cm -1 、1385cm -1 、1380cm -1 、1350cm -1 、1296cm -1 、1237cm -1 、1214cm -1 、1184cm -1 、1130cm -1 、1103cm -1 、1051cm -1 、1044cm -1 、1005cm -1 、978cm -1 、964cm -1 、860cm -1 、840cm -1 、810cm -1 、793cm -1 、781cm -1 、755cm -1 、741cm -1 、703cm -1 and 669cm -1 The peak.

11. The use of claim 1, wherein the crystalline form further comprises no more than about 20 mol% of the corresponding M isomer of compound 49a.

12. The use of claim 1, wherein the crystalline form further comprises no more than about 0.25 mol% of the corresponding M isomer of compound 49a.

13. The use of claim 1, wherein the crystalline form is substantially free of the corresponding M isomer of compound 49a.

14. The use of claim 1, wherein compound 49a has a chemical purity of about 95% or higher.

15. The use of claim 1, wherein the crystalline form of compound 49a contains no more than about 20 mol% of other solid forms.

16. The use of claim 1, wherein the crystalline form of compound 49a contains no more than about 0.25 mol% of other solid forms.

17. The use of claim 1, wherein the crystalline form of compound 49a is substantially free of other solid forms.

18. The use of claim 1, wherein the condition is an inflammatory condition.

19. Use according to claim 18, wherein the inflammatory condition is rheumatoid arthritis.

20. The use of claim 18, wherein the inflammatory condition is psoriatic arthritis.

21. The use according to claim 18, wherein the inflammatory condition is cryopyrine-associated periodic syndrome.

22. The use of claim 18, wherein the inflammatory condition is hidradenitis suppurativa.

23. The use of claim 18, wherein the inflammatory condition is selected from the group consisting of psoriasis, juvenile rheumatoid arthritis, ulcerative colitis, Crohn's disease, ankylosing spondylitis, gout, recurrent pericarditis, and idiopathic pulmonary fibrosis.

24. The use of claim 1, wherein the condition is cancer.

25. The use according to claim 24, wherein the cancer is selected from bone cancer, breast cancer and pancreatic cancer.

26. The use according to claim 25, wherein the breast cancer is metastatic breast cancer.

27. The use of claim 1, wherein the treatment further comprises administering one or more additional therapeutic agents in combination with the crystalline form of Compound 49.

28. The use of claim 27, wherein the one or more additional therapeutic agents are selected from the group consisting of anti-inflammatory drugs, immunosuppressive drugs, immunomodulatory drugs, cytostatic drugs, angiogenesis inhibitors, kinase inhibitors, cytokine blockers, inhibitors of cell adhesion molecules, chemotherapeutic agents, alkylating agents, antimetabolites, plant alkaloids and terpenoids, topoisomerase inhibitors, cytotoxic antibiotics, angiogenesis inhibitors, tyrosine kinase inhibitors and immune checkpoint inhibitors.

29. The use of claim 27, wherein the one or more additional therapeutic agents are selected from the group consisting of cisplatin, carboplatin, oxaliplatin, streptozocin, busulfan, cyclophosphamide, mercaptopurine, thioguanine, pentostatin, cytosine arabinoside, gemcitabine, fluorouracil, folinic acid, methotrexate, vincristine, vinblastine, paclitaxel, irinotecan, topotecan, etoposide, dactinomycin, doxorubicin, bleomycin, mitomycin, sunitinib, bevacizumab, imatinib, erlotinib, lapatinib, axitinib, atezolizumab, avelumab, durvalumab, ipilimumab, pembrolizumab, nivolumab and tesimumab.

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