Preparation method and application of American cockroach extract
By using gradually increasing cold-impregnation and ultrasonic reflux extraction technology in the American cockroach extraction process, the active ingredient content in the extract was successfully improved, and the problems of incomplete extraction of components and destruction of biologically active substances in the existing process were solved. Achieve efficient and side-effect treatment effect.
Patent Information
- Application Number
- CN202310997981.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-09
- Publication Date
- 2025-02-18
AI Technical Summary
The existing American cockroach extraction process cannot effectively extract active ingredients such as water-soluble amino acids and oligopeptides, and traditional alcohol extraction will destroy more biologically active substances, resulting in unsatisfactory efficacy of the extract and side effects.
Cold-immersion using an ethanol solution with a concentration from low to high, combined with ultrasonic reflux extraction technology, the free amino acids, oligopeptides and other active ingredients in the American cockroach were gradually extracted, and the concentration was reduced and the macropore adsorption resin was elution. A high content of active ingredients.
It significantly improves the active ingredient content in the American cockroach extract, improves the treatment effect on diabetic peripheral neuropathy, has obvious analgesic effects and no side effects, and has broad application prospects.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method and application of an American cockroach extract. Background Art
[0002] American cockroach (Periplaneta americana) is an insect of the Blattidae family and the genus Periplaneta, commonly known as "cockroach". It is widely distributed in my country and is a traditional Chinese medicinal material. It is often used as a dried or fresh adult. It is cold in nature, salty in taste, extremely smelly, and poisonous. It can strengthen the spleen and eliminate malnutrition, promote blood circulation and unblock meridians, promote diuresis and reduce swelling, and heal sores and promote tissue regeneration. It is used for hypochondriac pain, abdominal masses, malnutrition, palpitations, asthma, and edema. It is used externally to treat burns caused by water or fire, various wounds, and ulcers.
[0003] Diabetic peripheral neuropathy (DPN) is a microvascular complication of diabetes. The lesions involve the peripheral nervous system including sensory nerves, motor nerves and autonomic nerves. It is characterized by the gradual degeneration of peripheral nerves from far to near, resulting in the reduction or disappearance of sensory symptoms. Clinical features include pain, paresthesia and sensory loss. Paresthesias include numbness, ants, insects, fever, and electric shock-like sensations, which often extend from the distal toes to above the knees. Patients feel like wearing socks and gloves. At present, the pathogenesis of DPN has not been determined. Existing studies have shown that there is no other effective treatment except for lifestyle improvement and strict blood sugar control. Moreover, blood sugar control can only slow down the course of neuropathy in patients with type 1 diabetes, but it cannot improve type 2 DPN.
[0004] At present, the drugs used clinically to treat diabetic peripheral neuropathy include nerve growth factor, neurotrophic drug methylcobalamin, antiplatelet aggregation, vasodilator prostaglandin E1, cilostazol, oxidative stress inhibitor α-lipoic acid, pancreatic kallikrein, antidepressants, antiepileptic drugs, antipsychotic drugs, etc. However, the long-term effects of these drugs are not ideal and have side effects.
[0005] The existing American cockroach extraction process uses the traditional alcohol extraction method, and most of the water-soluble amino acids are not extracted. The invention patent "American cockroach medicinal extract extraction process" with patent number 031178049 uses: 1) inactivating the raw cockroaches with 65-75℃ hot water; 2) drying at 65-75℃, coarsely crushing and sieving; 3) soaking the raw material surface in 80-degree alcohol in a container for 48-50 hours. This method will destroy more biologically active substances. Summary of the invention
[0006] The first object of the present invention is to provide a method for preparing an American cockroach extract. The American cockroach extract prepared by the method of the present invention has a high content of active ingredients.
[0007] The method of the present invention comprises the following steps:
[0008] a, adding 45% to 60% ethanol solution to fresh American cockroach for the first cold soak, first ultrasonic reflux extraction, filtering to obtain medicinal residue 1, adding 65% to 80% ethanol solution to medicinal residue 1 for the second cold soak, second ultrasonic reflux extraction, filtering to obtain medicinal residue 2, adding 95% ethanol solution to medicinal residue 2 for the third cold soak, third ultrasonic reflux extraction, and filtering;
[0009] b. Combine the filtrates, concentrate under reduced pressure, and obtain an extract;
[0010] c. Pass the degreasing solution obtained by degreasing the extract through a macroporous adsorption resin, wash with water and ethanol solution to obtain an eluent, concentrate under reduced pressure, and dry.
[0011] The invention sequentially cold-immerses fresh American cockroaches in ethanol solutions with increasing concentrations, and uses ultrasonic reflux extraction technology to significantly increase the content of active ingredients such as free amino acids and oligopeptides in the American cockroaches. The medicine prepared by using the American cockroach extract of the invention has a significant effect in treating diabetic peripheral neuropathy.
[0012] Furthermore, in step a, during the first cold soaking, the amount of 45% to 60% ethanol solution added is 2 to 5 times the mass of fresh American cockroaches; and the cold soaking time is 6 to 8 hours.
[0013] For the second cold soaking, add 65% to 80% ethanol solution in an amount of 2 to 4 times the mass of the medicinal residue; the cold soaking time is 7 to 8 hours.
[0014] For the third cold soaking, the amount of 95% ethanol solution added is 2 to 3.5 times the mass of the residue 2; the cold soaking time is 6 to 7 hours.
[0015] Furthermore, the fresh American cockroaches are subjected to ultrasonic reflux extraction technology after each cold soak, that is, the power of the first ultrasonic reflux extraction is 450-650W, and the time is 60-90min. The power of the second ultrasonic reflux extraction is 500-600W, and the time is 45-60min. The power of the third ultrasonic reflux extraction is 500-600W, and the time is 45-60min. By controlling the parameters of the ultrasonic reflux extraction within the above range, more biologically active components in the American cockroaches can be extracted.
[0016] Furthermore, in step b, the temperature of the reduced pressure concentration is 60-70°C.
[0017] Furthermore, step c is to add purified water to the extract, then add gelatin solution, let it stand, remove precipitation and grease, obtain a degreasing solution, pass the degreasing solution through a macroporous adsorption resin, wash with water, wash with an ethanol solution with a concentration of 45 to 75% to obtain an eluent, concentrate under reduced pressure at 60 to 70°C to an extract with a relative density of 1.1 to 1.3 at 60°C, and dry to obtain the extract.
[0018] The invention also provides an American cockroach extract prepared by the method.
[0019] The present invention also provides a new medical use of the American cockroach extract prepared by the method, namely, use of the American cockroach extract in preparing a medicine for treating peripheral neuropathy.
[0020] Further, the neuropathy is related to diabetes.
[0021] Furthermore, the diabetes is type 1 diabetes or type 2 diabetes; preferably type 2 diabetes.
[0022] Furthermore, the peripheral neuropathy is diabetic peripheral neuropathy.
[0023] Wherein, the neuropathy is accompanied by any abnormal sensory symptom of ants crawling, burning, pinpricks, stabbing, jumping, knife cutting, and tearing.
[0024] The invention provides medical uses of an American cockroach extract. Pharmacological experiments show that the American cockroach extract has a certain improvement effect on the decrease of MNCV in diabetic rats and a certain shortening effect on the action potential refractory period. The American cockroach extract can prolong the time of the first licking of the foot of mice, indicating that the American cockroach extract can increase the pain threshold of mice, has a significant analgesic effect, has a certain effect of treating diabetic peripheral neuropathy, has no side effects, and has broad application prospects.
[0025] The invention provides a method for preparing an American cockroach extract. The method comprises the following steps: cold soaking fresh American cockroaches in ethanol solutions with increasing concentrations in sequence, and using an ultrasonic reflux extraction technology, so that more biologically active substances in the American cockroaches can be extracted, and the contents of active ingredients such as free amino acids and oligopeptides are significantly increased. DETAILED DESCRIPTION
[0026] The present invention will be further described in detail below through specific examples.
[0027] Example 1
[0028] Weigh 100g of fresh American cockroaches, add 5 times the concentration of 60% ethanol solution, soak for the first time for 8h, perform ultrasonic reflux extraction for the first time, the ultrasonic frequency is 650W, the ultrasonic time is 90min, and filter to obtain drug residue 1; add 4 times the mass of drug residue and 80% ethanol solution for the second cold soak for 7h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 600W, the ultrasonic time is 60min, and filter to obtain drug residue 2; add 3.5 times the mass of drug residue and 95% ethanol solution for the second cold soak for 8h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 600W, the ultrasonic time is 60min, and filter to obtain drug residue 2. % ethanol solution for the third time cold soaking for 7h, the third time ultrasonic reflux extraction, the ultrasonic frequency is 600W, the ultrasonic time is 60min, filtered, the filtrate is combined, and concentrated under reduced pressure at 70℃ to obtain an extract; purified water is added to the extract, and then gelatin solution is added, allowed to stand, precipitation and grease are removed to obtain a degreasing liquid, the degreasing liquid is passed through a macroporous adsorption resin, washed with water, and then washed with a 75% ethanol solution to obtain an eluent, which is concentrated under reduced pressure at 70℃ to an extract with a relative density of 1.2 at 60℃, and dried to obtain.
[0029] Example 2
[0030] Weigh 100g of fresh American cockroaches, add 2 times the concentration of 45% ethanol solution, soak for 6h for the first time, perform ultrasonic reflux extraction for the first time, the ultrasonic frequency is 450W, the ultrasonic time is 60min, and filter to obtain drug residue 1; add 2 times the mass of drug residue and 65% ethanol solution for the second cold soak for 7h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 500W, the ultrasonic time is 45min, and filter to obtain drug residue 2; add 2.5 times the mass of drug residue and 95% ethanol solution for the second cold soak for 7h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 500W, the ultrasonic time is 45min, and filter to obtain drug residue 2. % ethanol solution for the third time cold soaking for 6h, the third time ultrasonic reflux extraction, the ultrasonic frequency is 500W, the ultrasonic time is 45min, filtered, the filtrate is combined, and concentrated under reduced pressure at 60℃ to obtain an extract; purified water is added to the extract, and then gelatin solution is added, allowed to stand, precipitation and grease are removed to obtain a degreasing liquid, the degreasing liquid is passed through a macroporous adsorption resin, washed with water, and then washed with a 45% ethanol solution to obtain an eluent, which is concentrated under reduced pressure at 60℃ to an extract with a relative density of 1.1 at 60℃, and dried to obtain.
[0031] Example 3
[0032] Weigh 100g of fresh American cockroaches, add 4 times the concentration of 50% ethanol solution, soak for the first time for 7.5h, perform ultrasonic reflux extraction for the first time, the ultrasonic frequency is 500W, the ultrasonic time is 75min, and filter to obtain drug residue 1; add 3 times the mass of drug residue and 70% ethanol solution for the second time for 7.5h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 550W, the ultrasonic time is 50min, and filter to obtain drug residue 2; add 2.5 times the mass of drug residue and 9 The extract was concentrated under reduced pressure at 65°C to obtain an extract. Purified water was added to the extract, followed by gelatin solution, which was allowed to stand to remove precipitates and grease to obtain a degreasing solution. The degreasing solution was passed through a macroporous adsorption resin, washed with water, and then washed with a 60% ethanol solution to obtain an eluent. The eluent was concentrated under reduced pressure at 65°C to an extract with a relative density of 1.3 at 60°C, and dried to obtain the extract.
[0033] Comparative Example 1
[0034] 100 g of fresh American cockroaches are weighed, added with 5 times 95% ethanol solution, cold soaked for 10 hours, ultrasonic reflux extraction, ultrasonic frequency is 650W, ultrasonic time is 75 minutes, ultrasonic reflux extraction is performed 3 times, filtration is performed, filtrates are combined, and vacuum concentration is performed at 65°C to obtain an extract; purified water is added to the extract, and then a gelatin solution is added, and the extract is allowed to stand to remove precipitation and grease to obtain a defatted liquid, the defatted liquid is passed through a macroporous adsorption resin, washed with water, and then washed with a 60% ethanol solution to obtain an eluent, and vacuum concentrated at 65°C to an extract with a relative density of 1.3 at 60°C, and dried to obtain the extract.
[0035] Comparative Example 2
[0036] 100g of fresh American cockroaches are weighed, and an ethanol solution with a concentration of 50% is added to the mixture for the first cold soaking for 7.5h, and reflux extraction is performed at 70°C for 60min, and a residue 1 is obtained by filtering; an ethanol solution with a concentration of 65% which is twice the weight of the residue is added to the residue 1 for the second cold soaking for 7h, and reflux extraction is performed at 70°C for 50min, and a residue 2 is obtained by filtering; an ethanol solution with a concentration of 95% which is 2.5 times the weight of the residue is added to the residue 2 for the third cold soaking, and reflux extraction is performed at 70°C for 45min, and the mixture is filtered, the filtrate is combined, and the mixture is concentrated under reduced pressure at 60°C to obtain an extract; purified water is added to the extract, and then a gelatin solution is added, and the extract is allowed to stand to remove precipitation and grease to obtain a degreasing solution, the degreasing solution is passed through a macroporous adsorption resin, washed with water, and then washed with an ethanol solution with a concentration of 45% to obtain an eluent, and the eluent is concentrated under reduced pressure at 60°C to an extract with a relative density of 1.1 at 60°C, and the extract is dried.
[0037] Comparative Example 3
[0038] 100 g of fresh American cockroaches were weighed, and an ethanol solution with a concentration of 30% was added to the mixture for the first cold soaking for 5 h, and an ultrasonic reflux extraction was performed for the first time at a frequency of 400 W and a duration of 50 min. The residue 1 was filtered to obtain the residue 1; an ethanol solution with a concentration of 60% with a weight of 1.5 times the weight of the residue was added to the residue 1 for the second cold soaking for 6.5 h, and an ultrasonic reflux extraction was performed for the second time at a frequency of 450 W and a duration of 30 min. The residue 2 was filtered to obtain the residue 2; and an ethanol solution with a concentration of 60% with a weight of 1.5 times the weight of the residue was added to the residue 2 for the second cold soaking for 6.5 h. The extract was concentrated under reduced pressure at 65°C to obtain an extract. Purified water was added to the extract, followed by gelatin solution, and the extract was allowed to stand to remove precipitates and grease to obtain a degreasing solution. The degreasing solution was passed through a macroporous adsorption resin, washed with water, and then washed with a 60% ethanol solution to obtain an eluent. The eluent was concentrated under reduced pressure at 65°C to an extract with a relative density of 1.3 at 60°C, and dried to obtain the extract.
[0039] Comparative Example 4
[0040] Weigh 100g of fresh American cockroaches, add 6 times the concentration of 70% ethanol solution, soak for the first time for 8.5h, perform ultrasonic reflux extraction for the first time, the ultrasonic frequency is 700W, the ultrasonic time is 95min, and filter to obtain drug residue 1; add 5 times the mass of drug residue and 90% ethanol solution for the second cold soak for 9h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 650W, the ultrasonic time is 65min, and filter to obtain drug residue 2; add 4 times the mass of drug residue and 95% ethanol solution for the second cold soak for 9h, perform ultrasonic reflux extraction for the second time, the ultrasonic frequency is 650W, the ultrasonic time is 65min, and filter to obtain drug residue 2; % ethanol solution for the third time cold soaking for 8h, the third ultrasonic reflux extraction, the ultrasonic frequency is 650W, the ultrasonic time is 70min, filtered, the filtrate is combined, and concentrated under reduced pressure at 65℃ to obtain an extract; purified water is added to the extract, and then gelatin solution is added, allowed to stand, precipitation and grease are removed to obtain a degreasing liquid, the degreasing liquid is passed through a macroporous adsorption resin, washed with water, and then washed with a 60% ethanol solution to obtain an eluent, which is concentrated under reduced pressure at 65℃ to an extract with a relative density of 1.2 at 60℃, and dried to obtain.
[0041] Water was added to 500 mL of the extract obtained in Example 1-3 and Comparative Example 1-4, mixed, centrifuged for 30 min, the supernatant was filtered, and the filtrate was taken to determine the content of free amino acids and total water-soluble solids. The results are shown in Table 1.
[0042] Table 1 Contents of free amino acids and water-soluble total solids in the extracts obtained in the examples and comparative examples
[0043]
[0044] Example 4 Effect of American cockroach extract on sciatic nerve conduction velocity (MNCV) and action potential refractory period in rats with diabetic neuropathy
[0045] Experimental animals: Male Wistar rats, weighing 200-250 g.
[0046] Experimental drugs: streptozotocin (Dalian Meilun Biotechnology Co., Ltd., 18883-664); Tangmaikang granules (granules, 5 g per bag, 10 bags per box, Chengdu Zhonghui Pharmaceutical Co., Ltd., batch number 210614; dispensing method: directly take the granules and dissolve them with distilled water to the required concentration); American cockroach extract prepared in Example 1 of the present invention.
[0047] Test instruments: One-Touch blood glucose meter; BL-420 biofunction test system.
[0048] Experimental method: Male Wistar rats weighing 200-250g were fasted for 12h before the experiment, and then streptozotocin (STZ, 48mg / kg, prepared with citrate buffer before use) was injected intraperitoneally to establish the model. After 72h, the fasting blood glucose of the animals after fasting for 12h was measured using a One-Touch blood glucose meter. Rats with blood glucose levels >15mmol / L were diabetic rats.
[0049] Diabetic rats were randomly divided into model control group, positive control group (Tangmaikang), high-dose group of American cockroach extract, medium-dose group of American cockroach extract, and low-dose group of American cockroach extract. Another rat was not injected with streptozotocin as a normal control group, with 10 rats in each group. After 30 days of diabetes, 5 rats were taken from the model control group and the normal control group to measure MNCV. If the MNCV and action potential refractory period of the model group showed a trend of change, oral administration could be started once a day for 60 consecutive days.
[0050] After 30 days of administration, some rats in each group were taken to measure MNCV and action potential refractory period. The determination method and steps were as follows: after anesthesia, the rats were fixed in prone position, the right sciatic nerve was fully exposed, the stimulating electrode was placed at the sciatic nerve efferent site (proximal end) at the sciatic notch, and the recording electrode was placed at the medial malleolus of the tibia (distal end), connected to the BL-420 biofunctional experimental system, and appropriate stimulation parameters were selected (single pulse, wave width 0.1ms, stimulation intensity 1.5 times the threshold, stimulation interval more than 5s), and the room temperature was controlled (20±5℃) to maintain the body temperature of the rats at 37℃. The refractory period (latency) of the sciatic nerve action potential was recorded from the computer, and each rat was stimulated 3 times, and the average value was taken. The distance between the stimulating electrode and the recording electrode was accurately measured and substituted into the formula: sciatic nerve conduction velocity (m / s) = distance between the stimulating electrode and the recording electrode / action potential refractory period.
[0051] After 60 days of administration, the MNCV and action potential refractory period of the remaining rats in each group were observed, and the sciatic nerve was histologically observed. The results are shown in Tables 2 and 3.
[0052] The results showed that after 30 days of treatment, each dose group of American cockroach extract had a certain improvement effect on the decrease of MNCV in diabetic rats, and showed a certain shortening effect on the refractory period of action potential; after 60 days of administration, the high dose group of American cockroach extract could significantly shorten the refractory period of sciatic nerve action potential, and had a significant improvement effect on the slowing of MNCV.
[0053] Table 2 Effects of 30-day administration of American cockroach extract on sciatic nerve conduction velocity and action potential refractory period in diabetic mice ( )
[0054]
[0055] Compared with the normal control group: *P<0.05; **P<0.01; compared with the model control group: △P<0.05; △△P<0.01.
[0056] Table 3 Effects of American cockroach extract on sciatic nerve conduction velocity and action potential refractory period of diabetic mice after 60-day administration ( )
[0057]
[0058] Compared with the normal control group: *P<0.05; **P<0.01; compared with the model control group: △P<0.05; △△P<0.01.
[0059] Example 5 Analgesic effect of American cockroach extract
[0060] Experimental animals: Female Kunming mice, weighing 18-22 g.
[0061] Test drug: morphine hydrochloride injection (1 mL: 10 mg / 10 vials, Northeast Pharmaceutical Group Shenyang First Pharmaceutical Co., Ltd., batch number 210302); Add water to the American cockroach extract prepared in Example 1 to 1000 mL, mix well, and filter.
[0062] Test method: Use the hot plate method, place an aluminum tank in a 55±0.5℃ constant temperature water bath, preheat for 10 minutes, take one female mouse at a time, put it into the tank, and record the time (seconds / s) required for the mouse to lick its hind paw as the pain threshold of the mouse. Those that lick their paws for less than 5s or more than 30s or jump to escape will be discarded.
[0063] 72 qualified mice weighing 18-22g were randomly divided into a blank control group, a positive control group (morphine, intraperitoneal injection), and an American cockroach extract group (the drug was evenly applied to the mouse's feet), with 12 mice in each group. After grouping, each mouse was given the drug once a day for 3 consecutive days, and the blank control group was given an equal amount of saline (20ml / kg). The pain threshold was measured 30 and 60 minutes after the last administration. Each observation was 1 minute. If there was no pain response within 1 minute, the pain threshold was calculated as 60 seconds.
[0064] The results showed that the American cockroach extract could prolong the time of the first paw licking of mice at 30 and 60 minutes after administration, indicating that the American cockroach extract could increase the pain threshold of mice and have a significant analgesic effect, as shown in Table 4.
[0065] Table 4 Analgesic effect of American cockroach extract
[0066] Group dose Pain threshold 30 minutes after medication (s) Pain threshold 60 minutes after medication (s) Blank control group / 21±7.61 24±7.42 Positive control group 10mg / kg <![CDATA[42±11.48 ** ]]> <![CDATA[43±13.22 ** ]]> American cockroach extract 1mL / time <![CDATA[29±10.24 * ]]> <![CDATA[33±8.76 ** ]]>
[0067] Compared with the blank control group: *P<0.05; **P<0.01.
Claims
1. A method for preparing an American cockroach extract, characterized in that: The steps include: a, adding 45% to 60% ethanol solution to fresh American cockroach for the first cold soak, first ultrasonic reflux extraction, filtering to obtain medicinal residue 1, adding 65% to 80% ethanol solution to medicinal residue 1 for the second cold soak, second ultrasonic reflux extraction, filtering to obtain medicinal residue 2, adding 95% ethanol solution to medicinal residue 2 for the third cold soak, third ultrasonic reflux extraction, and filtering; b. Combine the filtrates, concentrate under reduced pressure, and obtain an extract; c. Pass the degreasing solution obtained by degreasing the extract through a macroporous adsorption resin, wash with water and ethanol solution to obtain an eluent, concentrate under reduced pressure, and dry.
2. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step a, during the first cold soaking, the amount of 45% to 60% ethanol solution added is 2 to 5 times the mass of fresh American cockroaches; and the cold soaking time is 6 to 8 hours.
3. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step a, during the second cold soaking, an ethanol solution with a concentration of 65% to 80% is added in an amount of 2 to 4 times the mass of the medicinal residue; and the cold soaking time is 7 to 8 hours.
4. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step a, during the third cold soaking, the amount of 95% ethanol solution added is 2 to 3.5 times the mass of the residue 2; the cold soaking time is 6 to 7 hours.
5. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step a, the power of the first ultrasonic reflux extraction is 450-650W, and the time is 60-90min; the power of the second ultrasonic reflux extraction is 500-600W, and the time is 45-60min; the power of the third ultrasonic reflux extraction is 500-600W, and the time is 45-60min.
6. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step b, the temperature for concentration under reduced pressure is 60-70°C.
7. The method for preparing the American cockroach extract according to claim 1, characterized in that: In step c, at least one of the following is satisfied: The degreasing treatment is to add purified water to the extract, then add gelatin solution, let it stand, remove precipitation and grease, and obtain a degreasing solution; The concentration of the ethanol solution is 45-75%; The temperature of the reduced pressure concentration is 60-70°C, and the extract is concentrated to a relative density of 1.1-1.3 at 60°C.
8. The American cockroach extract prepared by the method according to any one of claims 1 to 7.
9. Use of the American cockroach extract according to claim 8 in the preparation of a medicament for treating peripheral neuropathy.
10. The use according to claim 9, characterized in that: The peripheral neuropathy is diabetic peripheral neuropathy.