Solid-state nucleic acid register and method of data registration

Abstract

The application relates to the field of DNA computing, and discloses a solid-state nucleic acid register and a data storage method. The solid-state nucleic acid register comprises a solid-phase carrier and one or more planar origami structures fixed on the surface of the solid-phase carrier. The main surface of the planar origami structure and the main surface of the solid-phase carrier are fixedly connected to form a fixed relationship, another main surface of the planar origami structure is configured to write, store, read out and rewrite data, and the planar origami structure is self-assembled through base complementary pairing. The data storage method comprises the following steps: preparing the solid-state nucleic acid register, constructing a solid-state nucleic acid reaction tank, adding a DNA computing circuit component into the solid-state nucleic acid reaction tank and setting a data reading device, and performing data writing, storage, reading, rewriting of the DNA computing circuit in the solid-state nucleic acid reaction tank and observing by using the data reading device. The application provides a compact sequential digital circuit and improves the information processing efficiency.

Description

Technical Field

[0001] This application relates to the field of DNA computing technology, and more specifically to a solid-state nucleic acid register and a method for data storage. Background Technology

[0002] In the field of modern computing, DNA computing, as an emerging computational model, has attracted widespread attention due to its unique Watson-Crick base pairing properties. This is an innovative method for information processing and computation that utilizes the chemical properties and biological reactions of DNA molecules. Its core lies in its ability to achieve complex logical operations and data storage through precise intermolecular interactions. This computational model can not only process large amounts of data in parallel but also perform efficient information processing at the microscopic scale.

[0003] The advantages of DNA computing lie primarily in its high-density storage capacity and biocompatibility. Compared to traditional electronic computers, DNA molecules can store massive amounts of information in a smaller volume, offering a new approach to solving large-scale data problems. Furthermore, DNA computing systems exhibit superior energy efficiency in certain applications because they can perform calculations at room temperature, reducing energy consumption.

[0004] In recent years, researchers have leveraged the precise molecular interactions of DNA molecules to develop a variety of functions and applications, including logic circuits, neural network computing, and medical diagnostics. These innovations demonstrate the enormous potential of DNA computing in solving complex problems, particularly in parallel processing and efficient storage. However, DNA computing also faces challenges, such as slow response times, high error rates, and insufficient system scalability. These limitations affect the practicality of existing DNA computing technologies for handling complex tasks.

[0005] At the same time, DNA computing also needs to achieve sequential computation (a crucial component of information processing), enabling the system to efficiently process and transmit information by executing instructions or operations step by step. Sequential computation is an essential part of information processing, especially in classical computers and biological systems. In biology, cells regulate complex biological processes through the sequential expression of genes, while in computer science, the operation of electronic computers also relies on the processing of sequential information.

[0006] Currently, although various DNA-based sequential computing systems have been proposed, such as timers, oscillators, and state machines, these systems still face significant limitations when performing complex numerical calculations. Existing DNA computing technologies often can only handle relatively simple logical operations and struggle to cope with higher-order, complex computational tasks. Furthermore, existing systems typically require considerable time for information transmission. For example, data reading, writing, and transmission often rely on slow chemical reactions and physical movements, which limits the breadth and practicality of their applications. Even in the context of rapid research development, effectively improving the speed and complexity of DNA computing remains a major challenge. This not only affects the theoretical applications of DNA computing but also restricts its promotion and implementation in real-world scenarios. Therefore, in-depth exploration and overcoming these limitations will play a crucial role in promoting the development of DNA computing.

[0007] In summary, existing DNA computing technologies face a series of limitations when handling complex tasks, including slow response speed, high error rate, and insufficient system scalability. This typically limits these systems to performing only relatively simple logical operations, making them ineffective at handling higher-order, complex computational tasks. Furthermore, existing sequential computing systems often consume significant time during data reading, writing, and transmission, impacting their feasibility in practical applications. Summary of the Invention

[0008] The purpose of this application is to provide a solid-state nucleic acid register and a method for data storage, which provides a compact sequential digital circuit and improves the efficiency of information processing.

[0009] This application discloses a solid-state nucleic acid register, comprising:

[0010] A solid carrier, and one or more planar origami structures fixed to the surface of the solid carrier;

[0011] The main surface of the planar origami structure and the main surface of the solid carrier are solidly connected to form a fixed relationship. The other main surface of the planar origami structure is configured to write, store, read, and rewrite data.

[0012] The planar origami structure is formed through self-assembly of complementary base pairings.

[0013] In a preferred embodiment, it includes:

[0014] A fixing chain is provided at a specific position on the main surface of the planar origami structure, and the fixing chain is used to form a fixed connection with the molecules on the solid phase carrier.

[0015] A read / write chain is provided at a specific location on another main surface of the planar origami structure. The read / write chain is used for writing, storing, reading, and rewriting data.

[0016] In a preferred embodiment, it includes:

[0017] The planar origami structure has multiple channels or multiple regularly arranged regions, each channel or region being used for the independent writing, storage, reading and rewriting of one bit of data.

[0018] This application also discloses a method for data storage, including:

[0019] Step 101. Prepare the solid nucleic acid register as described above and construct the solid nucleic acid reaction vessel;

[0020] Step 102. Add a DNA computing circuit assembly and set up a data reading device to the solid nucleic acid reaction vessel;

[0021] Step 103. Perform data writing, storage, reading and rewriting of the DNA computing circuit in the solid nucleic acid reaction cell, and observe using the data reading device.

[0022] In a preferred embodiment, step 101 further includes:

[0023] Step 201. Clean, dry, modify and store the solid support for subsequent use;

[0024] Step 202. Add a molecular solution that can react with the fixed chain to form a fixed connection to the solid support and incubate for a period of time;

[0025] Step 203. Next, the planar origami structure is added to the solid-phase carrier to form the solid nucleic acid register, thus completing the preparation of the solid nucleic acid reaction cell, wherein the solid nucleic acid reaction cell is a reaction environment for data storage.

[0026] In a preferred embodiment, step 103, which involves writing, storing, reading, and rewriting data for the DNA computing circuit in the solid-state nucleic acid reaction vessel, further includes:

[0027] Step 301. Perform calculations for the solution-phase DNA circuit and write the calculation results;

[0028] Step 302. Read the calculation results stored in the solid-state nucleic acid register;

[0029] Step 303. Perform asynchronous cascading of the DNA circuit and write the new calculation results.

[0030] In a preferred embodiment, step 301 further includes:

[0031] The first input nucleic acid strand of the added DNA computing circuit component is added to the solid nucleic acid reaction vessel;

[0032] After a period of reaction, the DNA computing circuit component generates first output data with the first input nucleic acid strand, and then a strand substitution reaction is performed. The first output data is stored in the corresponding area of ​​the solid nucleic acid register and saved as the first data strand.

[0033] In a preferred embodiment, step 302 further includes:

[0034] Add a first release nucleic acid strand for reading out the stored data to a solid nucleic acid reaction vessel that already contains the calculation results;

[0035] The first data chain on the solid nucleic acid register is released into the solution through a chain substitution reaction by the first released nucleic acid chain, thus completing the reading of the first output data of the DNA computing circuit component.

[0036] In a preferred embodiment, step 303 further includes:

[0037] Add the corresponding downstream DNA circuit components to the solid nucleic acid reaction vessel containing the released first output data to generate the second output data;

[0038] Then, through a chain substitution reaction, the second output data is stored in the corresponding area of ​​the solid nucleic acid register and saved as the second data chain.

[0039] In a preferred embodiment, step 103 of observing using the data reading device further includes:

[0040] By fluorescently labeling the data chain corresponding to the calculation results generated in each step and recording the changes in fluorescence values ​​in real time using a total internal reflection fluorescence microscope, the visualization of the calculation results of the series circuit can be realized. The data reading device includes the total internal reflection fluorescence microscope.

[0041] The main differences and effects of the implementation method of this application compared with the prior art are as follows:

[0042] A compact sequential digital circuit was constructed, which improved the efficiency of information processing and enabled high-speed data transmission of sequential nucleic acid circuits.

[0043] This enhances system stability, reduces the impact of external interference on system accuracy, and lowers the error rate.

[0044] It expands computing power, enabling it to support more complex logical operations, thus allowing DNA computing technology to handle higher-order complex computing tasks and expanding its application scope.

[0045] It saves space and resources, reduces the required physical space and material consumption, and improves the overall efficiency of the system.

[0046] The specification of this application contains numerous technical features distributed across various technical solutions. Listing all possible combinations of these technical features (i.e., technical solutions) would make the specification excessively lengthy. To avoid this problem, the various technical features disclosed in the above-described invention, the various technical features disclosed in the following embodiments and examples, and the various technical features disclosed in the accompanying drawings can be freely combined to form various new technical solutions (all of which should be considered as described in this specification), unless such a combination of technical features is technically infeasible. For example, one example discloses feature A+B+C, and another example discloses feature A+B+D+E. Features C and D are equivalent technical means that serve the same function, and technically only one needs to be used; they cannot be used simultaneously. Feature E can technically be combined with feature C. Therefore, the solution A+B+C+D should not be considered as described because it is technically infeasible, while the solution A+B+C+E should be considered as described. Attached Figure Description

[0047] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0048] Figure 1 This is based on the structural characterization results of a solid-state nucleic acid register in one embodiment of this application.

[0049] Figure 2 This is based on the application of multiple storage sites in this application.

[0050] Figure 3 This is based on the application method of the chip compartment in this application.

[0051] Figure 4 This refers to the writing and reading of the results of a solution-phase DNA computing circuit according to one embodiment of this application;

[0052] Figure 5 This is a record of the calculation results based on the solution phase DNA calculation timing in one embodiment of this application. Detailed Implementation

[0053] In the following description, many technical details are presented to help the reader better understand this application. However, those skilled in the art will understand that the technical solutions claimed in this application can be implemented even without these technical details and various variations and modifications based on the following embodiments.

[0054] Explanation of some concepts:

[0055] Solution-phase DNA circuits: A type of circuit system constructed using DNA molecules in a solution environment. Unlike traditional electronic circuits based on solid-state materials such as silicon, these circuits utilize DNA molecules as the primary building blocks in a liquid solution phase. Circuit functions, such as information storage, signal processing, and transmission, are achieved through interactions between DNA molecules (e.g., complementary base pairing).

[0056] Registers: Components within the Central Processing Unit (CPU), these are high-speed storage devices with limited capacity used to temporarily store instructions, data, and addresses. They act like a small "workspace" for the CPU, playing a crucial role in the computer's data processing.

[0057] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below with reference to the accompanying drawings.

[0058] This application discloses a solid-state nucleic acid register, such as Figure 1 As shown, it includes:

[0059] A solid carrier, and one or more planar origami structures fixed to the surface of the solid carrier; wherein the main surface of the planar origami structure and the main surface of the solid carrier are solidly connected to form a fixed relationship, and another main surface of the planar origami structure is configured to write, store, read and rewrite data; and the planar origami structure is formed by self-assembly through complementary base pairing.

[0060] In this solid-state nucleic acid register design, a compact sequential digital circuit is constructed by resetting the state of the fixed solid-state nucleic acid registers, rather than physically moving them. Utilizing rewritable solid-state nucleic acid registers attached to a solid substrate, the output of a three-dimensional circuit is compressed onto a two-dimensional surface to improve information processing efficiency. After the circuit is updated, the data stored on the surface can be quickly read and transmitted to the computing system, thus enabling high-speed data transmission for sequential nucleic acid circuits.

[0061] In one embodiment, it includes:

[0062] The planar origami structure has fixed chains at specific locations on its main surface, which are used to form fixed connections with molecules on the solid support.

[0063] A read / write chain is located at a specific position on another main surface of the planar origami structure. The read / write chain is used for writing, storing, reading, and rewriting data.

[0064] Both the fixed chain and the read-write chain are staple chains.

[0065] In one embodiment, it includes:

[0066] The planar origami structure has multiple channels or multiple regularly arranged areas, each channel or area is used for the independent writing, storage, reading and rewriting of one bit of data.

[0067] like Figure 2 As shown, in one application, a single solid-state nucleic acid register can have over 200 addressable storage sites. Each storage site consists of a corresponding write region, address information, and read region. By artificially designing DNA sequences, it is possible to achieve single-molecule information storage across multiple regions and sites.

[0068] like Figure 3 As shown, in one application, by combining microfluidics and manually printing different chip compartments, different DNA computation sequences and high-throughput, large-scale DNA computations can be successfully achieved.

[0069] The advantages of the solid-state nucleic acid register design in this application include:

[0070] Improved computing speed: By eliminating physical movement operations, the information transmission time is significantly shortened, thus improving the overall computing speed.

[0071] Enhanced system stability: The fixed solid-state nucleic acid register remains stationary throughout the calculation process, reducing the impact of external interference on system accuracy and lowering the error rate.

[0072] This application also discloses a method for data storage, such as Figure 4 As shown, it includes:

[0073] Step 101. Prepare a solid nucleic acid register as described above and construct a solid nucleic acid reaction chamber; this includes pretreatment of the solid support. In the process of constructing the solid nucleic acid register, the pretreatment of the solid support is an important step to ensure that the planar origami structure can be effectively attached.

[0074] Step 102. Add DNA computing circuit components and set up a data reading device to the solid nucleic acid reaction vessel;

[0075] Step 103. Perform data writing, storage, reading and rewriting of the DNA computing circuit in a solid-state nucleic acid reaction vessel, and observe the data using a data reading device.

[0076] in, Figure 4 In the diagram, A represents a schematic of writing and reading data from a solid-state nucleic acid register. Figure 4 B in the diagram shows various fluorescence changes during the writing and reading of DNA data. Figure 4C in the image represents a fluorescence image of a single solid-state nucleic acid register at a specified time point over time. The blue channel shows the location of the solid-state nucleic acid register labeled with fluorescent ATTO-488, and the red channel shows the fluorescence of the Cy5-labeled data link.

[0077] In one embodiment, step 101 further includes:

[0078] Step 201. Clean, dry, modify, and store the solid support for subsequent use;

[0079] Step 202. Add a molecular solution that can react with the fixed chain to form a fixed connection on a solid support and incubate for a period of time;

[0080] Step 203. Next, a planar origami structure is added to the solid-phase carrier to form a solid nucleic acid register, thus completing the preparation of the solid nucleic acid reaction cell, which serves as the reaction environment for data storage.

[0081] In one embodiment, step 103, which involves writing, storing, reading, and rewriting data for the DNA computing circuit in a solid-state nucleic acid reaction vessel, further includes:

[0082] Step 301. Perform calculations for the solution-phase DNA circuit and write the calculation results;

[0083] Step 302. Read the calculation results stored in the solid-state nucleic acid register;

[0084] Step 303. Perform asynchronous cascading of the DNA circuit and write the new calculation results.

[0085] In one embodiment, step 301 further includes:

[0086] The first input nucleic acid strand of the added DNA computing circuit component is added to the solid nucleic acid reaction vessel;

[0087] After a period of reaction, the DNA computing circuit component generates the first output data with the first input nucleic acid strand, and then a strand substitution reaction is performed. The first output data is stored in the corresponding area of ​​the solid nucleic acid register and saved as the first data strand.

[0088] Then use the reaction solution to rinse away any excess liquid.

[0089] In one embodiment, step 302 further includes:

[0090] Add a first release nucleic acid strand to the solid nucleic acid reaction vessel that already contains the calculation results;

[0091] The first data strand on the solid nucleic acid register is released into the solution through a chain substitution reaction by the first released nucleic acid strand, thus completing the reading of the first output data of the DNA computing circuit component.

[0092] In one embodiment, step 303 further includes:

[0093] Add the corresponding downstream DNA circuit components to the solid nucleic acid reaction vessel containing the released first output data to generate the second output data;

[0094] Then, through a chain substitution reaction, the second output data is stored in the corresponding area of ​​the solid nucleic acid register and saved as the second data chain.

[0095] In one embodiment, step 103 of observing using a data reading device further includes:

[0096] By fluorescently labeling the data chain corresponding to the calculation results generated in each step and recording the changes in fluorescence values ​​in real time using a total internal reflection fluorescence microscope (TIRF), the visualization of the calculation results of the series circuit can be realized. The data reading device includes a total internal reflection fluorescence microscope.

[0097] The calculation result is as follows Figure 5 As shown, Figure 5 In this context, A represents a multilayer DNA circuit connected in series. Figure 5 In this context, B represents a single solid-state nucleic acid register where different regions can store different DNA calculation results. Figure 5 C in the diagram represents the truth table of the designed DNA timing circuit. Figure 5 In this context, D represents the calculation result of the first-level operational circuit. Figure 5 E in the equation represents the result of the entire circuit's calculations.

[0098] The advantages of the data storage method in this application include:

[0099] Expanding computing power: This architecture can support more complex logical operations, enabling DNA computing technology to handle higher-order complex computing tasks and expanding its application scope.

[0100] Space and resource savings: By compressing the output of three-dimensional circuitry onto a two-dimensional surface, the required physical space and material consumption are reduced, thereby improving the overall efficiency of the system.

[0101] In one embodiment, the planar origami structure in the solid-state nucleic acid register is square, the solid-phase support is a glass substrate, and the solid-phase connection is formed through a binding reaction between biotin and streptavidin. Step 101 further includes:

[0102] Step 401. Multiple cube-shaped planar origami structures are constructed through base complementary pairing; the use of classic cube origami as the basic model for solid nucleic acid registers is based on the physical characteristics of various DNA origami structures that have been successfully constructed and the needs of experiments.

[0103] Step 402. Set a fixed strand at a specific position on the main surface of the planar origami structure that can hybridize with the biotin-modified support short strand, and expose the extended part of the fixed strand below the bottom surface to ensure that it can stably hybridize with the biotin-modified support short DNA strand in the future.

[0104] Step 403. Set up a read / write chain at a specific location on another main surface of the planar origami structure, exposing the extension of the read / write chain on the surface to ensure that the data chain can be stored at a specific address in a stable hybrid manner;

[0105] Step 404. Solid nucleic acid registers are obtained by annealing assembly and polyethylene glycol purification. Specifically, address-designed staple chains are mixed with backbone chains and annealed to form addressable initial solid nucleic acid registers. The DNA origami assembly process is referenced in Rothemund, PWK, Folding DNA to Create Nanoscale Shapes and Patterns. Nature 2006, 440(7082), 297–302. Before use, the assembled solid nucleic acid registers are purified by PEG, and biotin-modified supporting DNA strands and blocking DNA strands for pre-sealing storage locations are added. After incubation at room temperature for 2 hours, polyethylene glycol (PEG) purification is performed again.

[0106] In one embodiment, for ease of observation, a glass substrate is selected as the solid-phase support, and step 201 further includes:

[0107] Step 501. Clean the glass substrate using an ultrasonic cleaner and cleaning agent to remove surface contaminants: The glass substrate needs to be thoroughly cleaned using an ultrasonic cleaner with an appropriate cleaning agent (such as a mixture of deionized water and ethanol) to remove any dust, grease and other contaminants that may be present on the surface.

[0108] Step 502. Immerse the cleaned glass substrate in a sodium hydroxide solution: The cleaned glass substrate needs to be immersed in a sodium hydroxide solution for further surface activation to improve its binding ability with biomolecules.

[0109] Step 503. Dry the glass substrate activated with sodium hydroxide; incubate the treated glass substrate in a silane solution to alkylate the surface of the glass substrate.

[0110] Step 504. Dry the surface-alkylated glass substrate; wherein, after incubation, the glass substrate is gently rinsed with deionized water and dried with high-purity nitrogen. Then it is placed in a high-temperature oven to completely dry the glass substrate, and finally stored in anhydrous ethanol to ensure that no water molecules adhere to the surface of the glass substrate during the process.

[0111] In one embodiment, for ease of observation, a glass substrate is selected as the solid-phase support, and the solid-phase connection is formed through a binding reaction between biotin and streptavidin. Step 202 further includes:

[0112] Step 601. Add polyethylene glycol or biotin-polyethylene glycol solution (PEG / Biotin-PEG solution) to the glass substrate and incubate for a period of time (usually 2 hours) to allow the Biotin-PEG molecules to fully react with and fix the glass substrate surface; after incubation, rinse with the reaction solution to remove excess free PEG / Biotin-PEG molecules from the solid nucleic acid reaction tank.

[0113] Step 602. Then, add an appropriate amount of streptavidin. The streptavidin molecules can bind to the biotin molecules on the Biotin-PEG and incubate for a period of time again. Then, use the reaction solution to wash away the free excess streptavidin molecules.

[0114] Step 603. Next, add a sufficient amount of solid nucleic acid registers to the glass substrate, incubate at room temperature, and then rinse again with the reaction solution. Through the above steps, the solid nucleic acid registers are fixed on the glass substrate, and the solid nucleic acid reaction tank is completed, which can provide a stable and controllable reaction environment.

[0115] In one embodiment, a read / write chain is provided at a specific location on a main surface of the planar origami structure, and step 103 further includes:

[0116] During a write operation, the first output data undergoes a chain swap reaction with the read / write chain;

[0117] During the readout operation, the first released nucleic acid strand undergoes a chain release reaction with the read / write strand.

[0118] It should be noted that in the claims and specification of this patent, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one" does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0119] All references to this application are incorporated herein by reference as if each reference were individually incorporated herein by reference. Furthermore, it should be understood that after reading the foregoing teachings of this application, those skilled in the art can make various alterations or modifications to this application, and these equivalent forms also fall within the scope defined by the appended claims.

[0120] In some cases, the actions or steps described in the claims can be performed in a different order than that shown in the embodiments and still achieve the desired result. Furthermore, the processes depicted in the drawings do not necessarily require a specific or sequential order to achieve the desired result. In some embodiments, multitasking and parallel processing are also possible or may be advantageous.

Claims

1. A solid state nucleic acid register, characterized in that, The solid-phase carrier and one or more planar origami structures fixed on the surface of the solid-phase carrier are included. The main surface of the planar origami structure and the main surface of the solid-phase carrier are fixedly connected to form a fixed relationship, another main surface of the planar origami structure is configured to perform data writing, storage, reading and rewriting of a DNA computing circuit, the DNA computing circuit includes a DNA timing circuit and supports logical operation; and the planar origami structure is self-assembled by base complementary pairing to form: The data writing, storage, reading and rewriting further include: Step 301. Perform computation of a solution-phase DNA circuit and writing of a computation result; Step 302. Read the computation result stored in the solid-state nucleic acid register; Step 303. Perform asynchronous concatenation of the DNA circuit and writing of a new computation result; The system can process and transfer information by executing instructions or operations step by step; Step 301 further includes: Adding a first input nucleic acid chain of the added DNA computing circuit component to the solid-state nucleic acid reaction tank; After a period of reaction, a first output data is generated by the DNA computing circuit component and the first input nucleic acid chain, and a strand displacement reaction is performed, the first output data is stored in the corresponding area of the solid-state nucleic acid register and saved as a first data chain; Step 302 further includes: Adding a first release nucleic acid chain for reading the stored data to the solid-state nucleic acid reaction tank where the computation result has been stored; Performing a strand displacement reaction by the first release nucleic acid chain to release the first data chain on the solid-state nucleic acid register into the solution, and completing reading of the first output data of the DNA computing circuit component; Step 303 further includes: Adding a corresponding downstream DNA circuit component to the solid-state nucleic acid reaction tank containing the released first output data to generate second output data; The second output data is stored in the corresponding area of the solid-state nucleic acid register and saved as a second data chain through a strand displacement reaction. The main surface of the planar origami structure is provided with a fixed chain at a specific position, and the fixed chain is used to form a fixed connection with molecules on the solid-phase carrier; 2. The solid-state nucleic acid register of claim 1, wherein, The other main surface of the planar origami structure is provided with a read-write chain at a specific position, and the read-write chain is used for writing, storing, reading and rewriting of a data chain; The solid-phase carrier is a glass substrate, and the fixed connection is formed by a binding reaction between biotin and streptavidin. The planar origami structure is provided with a plurality of channels or a plurality of regularly arranged areas, and each channel or each area is used for independent writing, storage, reading and rewriting of one bit of data. The planar origami structure is provided with a plurality of channels or a plurality of regularly arranged areas, and each channel or each area is used for independent writing, storage, reading and rewriting of one bit of data.

3. The solid-state nucleic acid register of claim 1, wherein, Step 101. Preparing the solid-state nucleic acid register according to any one of claims 1-3, and constructing a solid-state nucleic acid reaction tank; Step 102. Adding a DNA computing circuit component to the solid-state nucleic acid reaction tank and setting a data reading device; 4. A method of data registration, characterized by, Step 103. Performing data writing, storage, reading and rewriting of the DNA computing circuit in the solid-state nucleic acid reaction tank, and observing by using the data reading device. Step 101 further includes: ​ ​ 5. The method of claim 4, wherein, ​ Step 201. Cleaning, drying, modifying and preserving the solid phase carrier for subsequent use; Step 202. Adding a solution of molecules capable of forming a fixed connection after reacting with the fixed chain to the solid phase carrier and incubating for a period of time; Step 203. Then, adding the planar origami structure to the solid phase carrier to form the solid-state nucleic acid register, completing the preparation of the solid-state nucleic acid reaction tank, wherein the solid-state nucleic acid reaction tank is a reaction environment for data storage.

6. The method of claim 4, wherein, Step 201 further comprises: Step 501. Cleaning the glass substrate using an ultrasonic cleaner and cleaning agent to remove surface contaminants; Step 502. Immersing the cleaned glass substrate in a sodium hydroxide solution; Step 503. Drying the glass substrate after activation by sodium hydroxide; placing the treated glass substrate in a silane solution for incubation to alkylate the surface of the glass substrate; Step 504. Drying the glass substrate after surface alkylation.

7. The method of claim 4, wherein, The observation in step 103 using the data reading device further comprises: By fluorescently labeling the data chain corresponding to the calculation result generated at each step, and recording the change of fluorescence value in real time using a total internal reflection fluorescence microscope, the visual readout of the series circuit calculation result is realized, wherein the data reading device comprises the total internal reflection fluorescence microscope.

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