A body acne-removing composition containing guaiac extract and its application

Abstract

The present invention discloses a body acne-removing composition containing guaiac extract and its application, relating to the field of cosmetic technology. The present invention provides an acne-removing composition, comprising the following components in parts by weight: 0.01-0.1 parts of saponin A, 0.1-1 parts of saponin B, 5-15 parts of buckwheat extract, 1-10 parts of cassia seed extract, and 1-10 parts of guaiac extract; the saponin A includes calendulaside and aucubin, the saponin B is sanguisorbaside, and the sanguisorbaside includes sanguisorbaside I and sanguisorbaside II. When saponin B and saponin A, together with buckwheat extract, guaiac extract, and cassia seed extract, the acne-removing composition has an excellent effect on heat removal and detoxification, and can effectively prevent skin acne; on the other hand, it promotes the penetration of active substances in the skin barrier, relieves dry skin, accelerates the healing and repair of existing acne, and further improves the amount of skin oil and skin surface roughness.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a body acne-removing composition containing a guaiac extract and application thereof. Background Art

[0002] Acne is one of the most common skin diseases worldwide, affecting 85% of the population. At the pathophysiological level, two factors, sebaceous glands and Propionibacterium acnes, play a crucial role. Acne commonly occurs on the face, chest, shoulders, and upper back. Scars caused by acne, especially severe acne, can last a lifetime and have long-term psychosocial impacts. Therefore, a large number of local and systemic treatments are available for acne, but compared to facial treatments, there is a lack of effective and gentle treatment options for the body, especially the back.

[0003] Regarding the improvement of back acne, there are the following problems: (1) The skin on the back is not as easy to clean as the face, and the stratum corneum is thicker, and the metabolism is slower than that of the face. Excess oil in the body is blocked in the hair follicles and cannot be eliminated from the body, which makes it more likely to form acne. (2) The back is the reflex area of the lungs. If the lungs are hot or the meridians are blocked, it is easy for dampness to accumulate in the body and form toxins, causing acne on the back. A constitution prone to dryness and heat or endocrine disorders are very likely to cause acne. At present, most existing technologies use acidic substances to promote skin exfoliation, which is irritating and can easily damage the skin barrier, causing dullness or acne pits after acne. Substances that work on the face are not ideal for improving acne on the back skin, are expensive, and have poor practicality. In addition, most facial acne-removing ingredients do not have the effect of removing heat and detoxification, and the effect on acne removal on the back is not obvious.

[0004] Therefore, an acne-removing composition that has the effects of removing heat and detoxifying, reducing heat sensation, and can effectively prevent skin acne while accelerating the healing and repair of existing acne and improving acne pits and acne scars has broad application prospects. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a body acne-removing composition containing guaiac extract and its application, which has the effects of removing heat and detoxifying, and alleviating heat sensation, can effectively prevent skin acne, and at the same time can accelerate the healing and repair of existing acne, and improve acne pits and acne scars.

[0006] To achieve the above-mentioned object, the technical solution adopted by the present invention is: an acne-removing composition, comprising the following components in parts by weight: 0.01-0.1 parts of saponin A, 0.1-1 parts of saponin B, 5-15 parts of buckwheat extract, 1-10 parts of Cassia seed extract, and 1-10 parts of guaiac extract; the saponin A comprises calendulaside and aucubin, the structural formula of calendulaside is shown in formula (I), and the structural formula of aucubin is shown in formula (II); the saponin B is sanguisorbaside, the sanguisorbaside comprises sanguisorbaside I and sanguisorbaside II, the structural formula of sanguisorbaside I is shown in formula (III), and the structural formula of sanguisorbaside II is shown in formula (IV);

[0007]

[0008] Sanguisorba officinalis (SANGUISORBA OFFICINALIS), a member of the Rosaceae family, possesses numerous pharmacological properties, including antioxidant properties. Sanguisorbaside, the main active compound in Sanguisorba officinalis, exhibits diverse biological activities. Sanguisorbaside I and Sanguisorbaside II are classified. Sanguisorbaside I exhibits anti-inflammatory properties and promotes cell survival in vitro, while Sanguisorbaside II, a metabolite of Sanguisorbaside I, exhibits tumor-suppressing effects. Eucommia ulmoides (Eucommia barbata) contains over 100 active ingredients, including lignans, iridoids, phenylpropanoids, flavonoids, and polysaccharides. These ingredients exhibit diverse pharmacological effects, including lowering blood pressure, blood lipids, and blood sugar, as well as antibacterial, anti-inflammatory, antioxidant, and hepatoprotective and kidney-protective properties. Buckwheat extract, primarily derived from its seeds, leaves, and flowers, contains dietary fiber and flavonoids that not only have antioxidant properties but also help repair the skin barrier, particularly by improving skin hydration and enhancing its barrier function. Guaiac extract exhibits antibacterial and anti-inflammatory properties, promotes tissue regeneration, and repairs the skin barrier.

[0009] The inventors of the present application found in the actual experimental process that when saponin B (including sanguisorbaside I and sanguisorbaside II) and saponin A (including scutellarin and aucubin) work together, the structural similarity gives them affinity for targets in the organism, and the mechanism of action of saponin B and saponin A can complement each other, enhancing the overall effect of the anti-acne composition in expelling heat and detoxification, and alleviating heat sensation, thereby effectively preventing skin acne; and the sugar part of the saponin compound helps the substance penetrate the skin barrier. The present invention can further improve the effect of expelling heat and detoxification, and alleviating heat sensation without increasing side effects by rationally allocating the weight parts of saponin B and saponin A. The rational allocation of saponin B and saponin A helps the active substance penetrate the skin barrier and is suitable for back skin. The inventors of the present application found in the actual experimental process that the combination of buckwheat extract, guaiac extract, and cassia seed extract can further relieve skin allergies and redness, improve skin tolerance, and reduce allergic reactions. Therefore, when saponins B (including sanguisorbaside I and sanguisorbaside II) and saponins A (including aucubin and aucubin) are combined with buckwheat extract, guaiac extract, and cassia seed extract, the anti-acne composition is effective in removing heat and detoxifying, alleviating heat sensation, and effectively preventing acne. Furthermore, it promotes the penetration of active substances through the skin barrier, alleviates dry skin, accelerates the healing and repair of existing acne, and further improves skin oil content and surface roughness. These components work together to improve the acne microenvironment and alleviate acne pits and scars.

[0010] Preferably, the anti-acne composition comprises the following components in parts by weight: 0.05-0.08 parts of saponin A, 0.3-0.5 parts of saponin B, 8-12 parts of buckwheat extract, 6-8 parts of cassia seed extract, and 5-8 parts of guaiac extract.

[0011] Preferably, in the anti-acne composition, the mass percentage of saponin A is 0.25-0.28%.

[0012] Preferably, in the anti-acne composition, the mass percentage of saponin B is 1.3-1.8%.

[0013] Preferably, in the anti-acne composition, the total mass percentage of saponin A and saponin B is 1.6-2.1%.

[0014] The inventors of the present application found in the actual experimental process that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the mass parts of the components are further selected within the above range, the obtained product has better heat removal and detoxification effects, reduces heat sensation and prevents skin acne, and further promotes the penetration of active substances in the skin barrier, relieves skin allergies and redness and swelling, and enhances the repair effect on existing acne.

[0015] Preferably, the weight ratio of the aucubin to the aucubin is 1:(1.8-4.2); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (11-14):1.

[0016] Further preferably, the weight ratio of the aucubin to the aucubin is 1:(2-2.5); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (12-13):1.

[0017] The inventors of the present application found in the actual experimental process that when the saponin A includes aucubin and aucubin, and aucubin and aucubin are in the above-mentioned specific weight ratio, the obtained product has better heat removal and detoxification effects, reduces heat sensation and prevents skin acne, and further promotes the penetration of active substances in the skin barrier, relieves skin allergies and redness, and enhances the repair effect on existing acne.

[0018] The inventors of the present application found during the actual experimental process that when the saponin B includes Sanguisorbaside I and Sanguisorbaside II, and Sanguisorbaside I and Sanguisorbaside II are in the above-mentioned specific weight ratio, the obtained product has better heat removal and detoxification effects, reduces heat sensation and prevents skin acne, and further promotes the penetration of active substances in the skin barrier, relieves skin dryness, accelerates the healing and repair of existing acne, and further improves the amount of skin oil and skin surface roughness.

[0019] In addition, the present invention provides use of the anti-acne composition in preparing skin products.

[0020] Furthermore, the present invention provides a skin care product comprising the acne-removing composition. Optionally, the skin care product comprises at least one of a toner, lotion, cream, mask, essence, body lotion, and spray.

[0021] Optionally, the present invention provides a skin product comprising the following components in percentage by mass: 1-5% of an anti-acne composition, 5-40% of a cosmetic base, and the balance being water; the cosmetic base comprising at least one of a moisturizer, a thickener, an emulsifier, a skin conditioner, an antioxidant, and a moisturizer.

[0022] Optionally, the present invention provides an anti-acne body lotion comprising the following components in percentage by weight: 1-5% of an anti-acne composition, 1-10% of a moisturizer, 0.1-0.5% of a thickener, 0.5-5% of an emulsifier, 0.05-1% of a skin conditioner, 0.5-1% of an antioxidant, 2-12% of an emollient, and the balance being deionized water.

[0023] Exemplarily, the moisturizer includes at least one of glycerin, butylene glycol, low molecular weight sodium hyaluronate, and 1,2-hexanediol; the thickener includes at least one of xanthan gum, carbomer 980, high molecular weight cellulose, and polyacrylate crosspolymer-6; the emulsifier includes at least one of C12-20 alkyl glucoside, C14-22 alcohol, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and a mixture of arachidyl alcohol / behenyl alcohol / arachidyl glucoside; the skin conditioning agent includes at least one of trehalose, allantoin, and hyaluronic acid; the antioxidant includes at least one of tocopheryl acetate, p-hydroxyacetophenone, and ascorbic acid; the emollient includes at least one of polydimethylsiloxane, shea butter (BUTYROSPERMUM PARKII) butter, caprylic / capric triglyceride, petrolatum, and stearyl alcohol;

[0024] Compared with the prior art, the present invention has the following beneficial effects: when saponin B (including sanguisorbaside I and sanguisorbaside II) and saponin A (including aucubin and aucubin) are synergistically combined with buckwheat extract, guaiac extract, and cassia seed extract, the anti-acne composition as a whole is excellent in removing heat and detoxifying, alleviating heat sensation, and effectively preventing acne. Furthermore, it promotes the penetration of active substances through the skin barrier, alleviates dry skin, accelerates the healing and repair of existing acne, and further improves skin oil content and surface roughness. These components work together to improve the acne microenvironment and alleviate acne pits and scars. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Figure 2 is a diagram of the swimming trajectory of zebrafish embryos in the thermal sensation test; Figure (a) is a diagram of the swimming trajectory of zebrafish embryos in Example 6, and Figure (b) is a diagram of the swimming trajectory of zebrafish embryos in Comparative Example 1; Figure (c) is a diagram of the swimming trajectory of zebrafish embryos in the positive control group; and Figure (d) is a diagram of the swimming trajectory of zebrafish embryos in the model control group.

[0026] Figure 2 This is a comparison of the papules on the back skin before and after 28 days of use in Application Example 6;

[0027] Figure 3 This is a comparison of back skin acne before and after 28 days of use in Application Example 6. DETAILED DESCRIPTION

[0028] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0029] The raw materials used in the present invention are further described:

[0030] Synechoside: purchased from J&K Technology Co., Ltd.

[0031] Aucubin: purchased from Bailingwei Technology Co., Ltd.;

[0032] Sanguisorba officinalis saponin I: purchased from Shanghai Yien Chemical Technology Co., Ltd.;

[0033] Sanguisorba officinalis saponin II: purchased from J&K Technology Co., Ltd.;

[0034] Buckwheat extract: product number SXHLWWZTQW, purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.

[0035] Cassia seed extract: product number L1-CY1139, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0036] Guaiac wood extract: Product No. L2-DZ-SCB3675-X, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0037] Barley extract: Product No. FDGS-009, purchased from Xi'an Feida Biotechnology Co., Ltd.

[0038] Redwood seed extract: product number HMZ, purchased from Xi'an Prius Bioengineering Co., Ltd.;

[0039] Extract of Mirrabiaceae: product number 000001, purchased from Shanxi Yirun Biotechnology Co., Ltd.

[0040] Commercially available Eucommia ulmoides: purchased from Shanghai Yuanye Biotechnology Co., Ltd. (the ratio of aucubin to aucubin is 1:5.5)

[0041] Commercially available Sanguisorba officinalis: purchased from Haozhou Jinlin Pharmaceutical Co., Ltd. (the ratio of Sanguisorba officinalis saponin I to Sanguisorba officinalis saponin II is 19:1)

[0042] The content ratio of aucubin and calophyllin in commercially available Eucommia ulmoides was detected by HPLC.

[0043] The method was as follows: an appropriate amount of Eucommia ulmoides powder was added to a round-bottom flask, 25 times the volume of 50% methanol was added, and ultrasonic extraction was performed for 40 minutes. The extract was then filtered through a 0.22μ microporous membrane filter, and the filtrate was stored. HPLC conditions were as follows: column: Thermo Hypersil Gold C18 (250 mm × 4.6 mm × 5 μm); mobile phase: methanol (A)-0.5% phosphoric acid solution (B), gradient elution (0-30 min, A: 5% → 10%, B: 95% → 90%; 30-70 min, A: 10% → 25%, B: 90% → 75%); detection wavelengths: 206 nm (0-15 min), 236 nm (15-55 min), and 360 nm (55-120 min); injection volume: 5 μL; flow rate: 1.0 mL / min. The results showed that the ratio of aucubin to aucubin was 1:5.5.

[0044] The content ratio of sanguisorbaside I and sanguisorbaside II in commercially available Sanguisorba officinalis was detected by HPLC.

[0045] The method is as follows: Take an appropriate amount of Sanguisorba officinalis powder, add 100% 80% methanol, sonicate for 40 minutes, filter through a 0.22μ microporous filter, and save the filtrate. HPLC conditions are as follows: Column: Thermo Synoronis C18 column (4.6mm×250mm, 5μm); Mobile phase: 0.1% phosphoric acid water as mobile phase A, acetonitrile as mobile phase B, gradient elution: 0-13 min, 65% A; 13-22 min, 65%→50% A; 22-40 min, 50% A. Flow rate: 1.0 mL / min; Detection wavelength: 203 nm; Column temperature: 35°C; Injection volume: 10 μL. The results showed that the ratio of Sanguisorba officinalis saponin I to Sanguisorba officinalis saponin II was 19:1.

[0046] Examples and Comparative Examples

[0047] Examples 1-16

[0048] The present invention provides an anti-acne composition. The components and weight portions of the anti-acne composition are shown in Table 1. The preparation method of the anti-acne composition comprises the following steps:

[0049] Saponin B is dissolved in 1.2 times the amount of glycerin and ultrasonically dissolved; saponin A, buckwheat extract, cassia seed extract, and guaiac extract are dissolved in 2 times the amount of distilled water and ultrasonically dissolved; the above substances are ultrasonically mixed to obtain the acne-removing composition.

[0050] Example 17

[0051] Provided is an acne-removing composition, comprising the following components in parts by weight: 3 parts of commercially available Eucommia ulmoides, 7.5 parts of commercially available Sanguisorba officinalis, 10 parts of buckwheat extract, 8 parts of Cassia seed extract, and 7 parts of Guaiac extract;

[0052] The preparation method is as follows: weigh eucommia bark, sanguisorba officinalis, buckwheat extract, cassia seed extract, and guaiac extract according to weight, and mix them evenly to obtain the anti-acne composition.

[0053] Table 1

[0054]

[0055]

[0056] Comparative Examples 1-10

[0057] The comparative example of the present invention provides an anti-acne composition. The components and weight portions of the anti-acne composition are shown in Table 2. The preparation method of the anti-acne composition comprises the following steps:

[0058] Saponin B is dissolved in 1.2 times the amount of glycerin and ultrasonically dissolved; saponin A, buckwheat extract, cassia seed extract, and guaiac extract are dissolved in 2 times the amount of distilled water and ultrasonically dissolved; the above substances are ultrasonically mixed to obtain the acne-removing composition.

[0059] If there is no relevant component, just do not add it. If there is a replacement component, the preparation method is the same.

[0060] Comparative Example 1

[0061] The present invention provides an acne-removing composition. Compared with Example 6, only the selection of saponin A is different, and aucubin is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of calendulaside, 0.35 parts of saponin B (sanguisorba officinalis saponin I: sanguisorba officinalis saponin II = 13:1), 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0062] Comparative Example 2

[0063] The present invention provides an acne-removing composition. Compared with Example 6, only the selection of saponin A is different, and saponin is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of aucubin, 0.35 parts of saponin B (sanguisorbaside I:sanguisorbaside II=13:1), 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0064] Comparative Example 3

[0065] The present invention provides an acne-removing composition. Compared with Example 6, the only difference is the selection of sanguisorbaside, and sanguisorbaside II is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of saponin A (calendulaside:aucubin=1:2.1), 0.35 parts of sanguisorbaside I, 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0066] Comparative Example 4

[0067] The present invention provides an acne-removing composition. Compared with Example 6, the only difference is the selection of sanguisorbaside, and sanguisorbaside I is not included. Specifically, the composition comprises the following components in parts by weight: 0.07 parts of saponin A (calendulaside:aucubin=1:2.1), 0.35 parts of sanguisorbaside II, 10 parts of buckwheat extract, 8 parts of cassia seed extract, and 7 parts of guaiac extract.

[0068] Table 2

[0069]

[0070]

[0071] Application Examples

[0072] The application examples of the present invention provide a body lotion, the components (mass percentage) of the body lotion are shown in Table 3; wherein the compositions in Application Examples 1-17 are respectively the anti-acne compositions prepared in Examples 1-17; the anti-acne compositions in Comparative Application Examples 1-10 are respectively the anti-acne compositions prepared in Comparative Examples 1-10; the blank application examples do not contain only the anti-acne composition; for example, the anti-acne composition used in Application Example 1 is the anti-acne composition in Example 1, the anti-acne composition used in Application Example 5 is the anti-acne composition in Example 5, the anti-acne composition used in Application Example 15 is the anti-acne composition in Example 15, and the same applies to other examples; the anti-acne composition used in Application Example 18 is the anti-acne composition prepared in Example 6.

[0073] Table 3

[0074]

[0075]

[0076] The preparation method of the body lotion comprises the following steps:

[0077] (1) Component A is mixed with water, heated to 80°C, and homogenized at 1200 rpm for 5 minutes. After homogenization, the mixture is kept warm for later use to obtain prefabricated component A;

[0078] (2) Mix component B, heat to 80°C, and homogenize at 1200 rpm for 5 minutes. After homogenization, keep warm and set aside to obtain prefabricated component B;

[0079] (3) Mix the C components and heat to 60°C to melt to obtain a prefabricated C component;

[0080] (4) Heat the preformed component A to 80°C, add the preformed component B at a speed of 300 rpm, stir and mix, then cool to 60°C, add the preformed component C at a speed of 300 rpm, stir and mix, then cool to below 45°C, add the acne-removing composition and continue stirring for 5 minutes, then stop stirring, discharge, and obtain an emulsion.

[0081] Performance Test-1 Acne-related Pathogen Inhibition Test

[0082] This effect example explores the inhibitory effect of the acne-removing composition prepared by Examples 1-17 and Comparative Examples 1-10 on Propionibacterium acnes. The standard strain of lyophilized Propionibacterium acnes ATCC 6919 was purchased from the Guangdong Provincial Microbial Culture Collection Center. Using the 96-well plate crystal violet staining method, the acne-removing composition was added to a certain concentration of bacterial solution so that its final dosage concentration was 100 μg / mL. It was cultured under anaerobic conditions at 37°C for 7 days, the upper planktonic cells were discarded, washed with sterile water, and then added with formaldehyde for 10 minutes. After removal, it was air-dried for 5 minutes. The biofilm was stained with 0.1% crystal violet for 20 minutes. After removing the dye, 95% ethanol was added for decolorization for 15 minutes. 100 μL of the decolorized solution was taken in a blank well plate and the absorbance was measured at a wavelength of 570 nm using an enzyme reader. The biofilm of the control group without the addition of the composition was 100%. The percentage of biofilm formation in each experimental group was calculated, and the results are shown in Table 4.

[0083] Table 4

[0084]

[0085]

[0086] Note: Compared with the blank control group, significance is indicated by *, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.001 is indicated by ***, and P value > 0.05 is indicated by ns.

[0087] The main pathogenic bacteria of acne, Propionibacterium acnes, plays a key role in pathogenicity. Inhibiting or killing Propionibacterium acnes is of great significance for the early prevention and treatment of acne. This experiment tests the inhibition rate of Propionibacterium acnes biofilm to evaluate the antibacterial ability of the acne-removing composition. Figure 2-3It can be seen that the biofilm formation rate of the acne-removing composition prepared in the embodiment of the present invention is below 70%, indicating that the acne-removing composition of the present invention has a significant inhibitory effect on Propionibacterium acnes. Figure 2 This is a comparison of the papules on the back skin before and after 28 days of use in Application Example 6; Figure 3 This is a comparison of back skin acne before and after 28 days of use in Application Example 6.

[0088] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, and guaiac extract 5-8 parts", the obtained product has a better inhibitory effect on Propionibacterium acnes.

[0089] From the comparison between Example 1 and Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, the product obtained has a better inhibitory effect on Propionibacterium acnes when aucubin and aucubin are in a specific weight ratio of "the weight ratio of aucubin to aucubin is 1: (2-2.5)".

[0090] From the comparison between Example 1 and Examples 12-16, it can be seen that saponin B includes sanguisorbaside I and sanguisorbaside II. When sanguisorbaside I and sanguisorbaside II are in a specific weight ratio of "the weight ratio of sanguisorbaside I to sanguisorbaside II is (12-13):1", the product obtained has a better inhibitory effect on Propionibacterium acnes.

[0091] From the comparison between the examples and the comparative examples, it can be seen that the saponin B and saponin A of the present invention, as well as the buckwheat extract, the cassia seed extract and the guaiac extract act synergistically and none of them can be missing.

[0092] Performance Test-2 Thermal Test

[0093] Acne can cause facial flushing or long-term redness, and damp heat in the body is also one of the important factors that cause acne on the body. This effect example explores the improvement effect of the anti-acne composition prepared by Example 1-17 and Comparative Example 1-10 on thermal sensation. Zebrafish have a capsaicin pathway, and can also be activated at high temperatures, reaching a peak at 37°C or above, which is manifested as a significant increase in swimming speed. The application software measures the movement distance of zebrafish embryos, compares the changes in the movement distance of zebrafish embryos in the test substance treatment group and the model control group, and calculates the movement distance shortening rate to evaluate the effect of the anti-acne composition in reducing thermal sensation. The spawning test of wild-type AB strain zebrafish (Danio rerio) from a reliable source (China National Zebrafish Resource Center) was used. The breeding water temperature should be controlled at 26-28.5°C, and the indoor temperature is recommended to be controlled at 20-25°C. After the eggs were removed from the egg collection box, they were cleaned with fish embryo culture medium. Healthy zebrafish embryos were selected and cultured at 28°C with a density of no more than one fish embryo in 200 μL of fish embryo culture medium until 5 days after fertilization.

[0094] The anti-acne composition and dimethyl sulfoxide were mixed at a ratio of 1:1 (weight g: volume mL), ultrasonically treated for 10 minutes, added to fish embryo culture medium to a concentration of 0.13%, shaken for 30 seconds, and centrifuged at 6500 rpm for 10 minutes. The supernatant was collected for testing.

[0095] The test was divided into groups: a model control group (fish embryo culture medium), a positive control group (0.05 g / L 4-tert-butyl cyclohexanol solution prepared with fish embryo culture medium), and a test substance group (acne-removing composition).

[0096] Model control group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of fish embryo culture medium.

[0097] Positive control group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of 4-tert-butylcyclohexanol solution;

[0098] Test group: 12 fish embryos were randomly selected and transferred to a 96-well plate, with each well containing one fish embryo and 0.2 mL of the acne-removing composition.

[0099] Each group was placed in a 28±1°C incubator for 2 hours. The zebrafish were then placed in a preheated zebrafish motion recorder (DanioVision, Noldus Information Technology) at 38°C. The movement trajectory of each zebrafish embryo was recorded in the dark for 5 minutes using infrared tracking software EthoVision XT. The 0.18 mL solution in each well was replaced with embryo culture medium. The embryos were then placed in a 28°C incubator for 24 hours. Embryo survival was then calculated. Pixel intensity in each well was measured as the distance traveled by each embryo.

[0100] Calculate the shortening rate of movement distance: shortening rate = (CT) / C × 100%; where: T is the average movement distance of fish embryos in the test substance treatment group; C is the average movement distance of fish embryos in the model control group; a two-tailed T test was performed on the average movement distance of fish embryos in the test substance treatment group and the average movement distance of the model control group to obtain the P value.

[0101] The results are shown in Table 5.

[0102] Table 5

[0103]

[0104]

[0105] Note: Compared with the model control group, significance is indicated by *, P value < 0.05 is indicated by *, P value < 0.01 is indicated by **, P value < 0.001 is indicated by ***, and P value > 0.05 is indicated by ns.

[0106] Figure 1 Figure 2 is a diagram of the swimming trajectory of zebrafish embryos in the thermal sensation test; Figure (a) is a diagram of the swimming trajectory of zebrafish embryos in Example 6, and Figure (b) is a diagram of the swimming trajectory of zebrafish embryos in Comparative Example 1; Figure (c) is a diagram of the swimming trajectory of zebrafish embryos in the positive control group; and Figure (d) is a diagram of the swimming trajectory of zebrafish embryos in the model control group.

[0107] From the experimental results in Table 5, it can be seen that the movement distance reduction rate of the acne-removing composition prepared in the embodiment of the present invention is above 65%, indicating that the acne-removing composition of the present invention has a better effect on improving thermal sensation.

[0108] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the mass parts of the components are further selected within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, and guaiac extract 5-8 parts", the obtained product has a better effect on improving the thermal sensation.

[0109] From the comparison between Example 1 and Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, and the weight ratio of aucubin and aucubin is "the weight ratio of aucubin to aucubin is 1: (2-2.5)", the obtained product has a better effect on improving the thermal sensation.

[0110] From the comparison of Example 1 and Examples 12-16, it can be seen that saponin B includes sanguisorbaside I and sanguisorbaside II. When sanguisorbaside I and sanguisorbaside II are in a specific weight ratio of "the weight ratio of sanguisorbaside I to sanguisorbaside II is (12-13):1", the product obtained has a better effect on improving the thermal sensation.

[0111] It can be seen from the comparison of the embodiments and comparative examples that saponin B and saponin A of the present invention, as well as the buckwheat extract, Cassia seed extract and Guaiac extract act synergistically and none of them can be missing.

[0112] Performance Test-3 Detection of back skin acne count, oiliness, and smoothness

[0113] The effectiveness examples of the present invention verify the efficacy of application examples 1-18, comparative application examples 1-10 and blank application examples when applied to human bodies, specifically including the following method: according to the "Technical Specifications for Safety of Cosmetics" (2015), subjects aged 18-60 were selected. Under normal circumstances, the subjects were subjected to a randomized trial with 8 people in each group, for a total of 29 groups. The test was continued for 28 days, and instrumental detection and clinical evaluation of skin indicators were performed on the 0th, 7th, 14th and 28th days of the test.

[0114] The specific steps of instrument measurement are as follows: After bathing every night, the subject takes 1.5mL of body lotion and applies it to the acne-prone area on the back, and massages it for absorption. On the day of the visit, the subject cleans with clean water without applying any products, and sits quietly in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. Instruments are used to quantify and photograph the skin condition, including VC20Plus to measure the roughness, Sebumeter SM 815 to test the amount of oil, and acne counts (papules, comedones) evaluated by a doctor. Record the data and calculate the index improvement rate on the 28th day, index improvement rate = (value before use - value after use) / value before use × 100%.

[0115] The results are shown in Table 6.

[0116] Table 6

[0117]

[0118]

[0119] Note: Compared with the blank control group, significance is indicated by *, P value < 0.05 is indicated by *, and P value > 0.05 is indicated by ns.

[0120] The experimental results in Table 6 demonstrate that the acne-removing composition prepared in this embodiment of the present invention significantly improves papules, acne, oiliness, and skin roughness on the back, demonstrating that the body lotion prepared in this invention plays a significant role in improving and preventing acne and smoothing the skin. The aforementioned components work synergistically to improve the acne microenvironment and alleviate acne pits and scars.

[0121] From the comparison of Examples 1-6, it can be seen that the weight parts of saponin A, saponin B, buckwheat extract, cassia seed extract, and guaiac extract will affect the performance of the product. When the weight parts of the components are further selected within the range of "saponin A 0.05-0.08 parts, saponin B 0.3-0.5 parts, buckwheat extract 8-12 parts, cassia seed extract 6-8 parts, and guaiac extract 5-8 parts", the obtained body lotion has a better effect on improving papules, acne, oil and skin roughness on the back skin.

[0122] From the comparison between Example 1 and Examples 7-11, it can be seen that when the saponin A includes aucubin and aucubin, and the weight ratio of aucubin and aucubin is "the weight ratio of aucubin to aucubin is 1: (2-2.5)", the obtained body lotion has a better effect on improving papules, acne, oil and skin roughness on the back skin.

[0123] From the comparison of Example 1 and Examples 12-16, it can be seen that saponin B includes Sanguisorbaside I and Sanguisorbaside II. When Sanguisorbaside I and Sanguisorbaside II are in a specific weight ratio of "the weight ratio of Sanguisorbaside I to Sanguisorbaside II is (12-13):1", the resulting body lotion has a better effect on improving papules, acne, oil and skin roughness on the back skin.

[0124] It can be seen from the comparison of the embodiments and comparative examples that saponin B and saponin A of the present invention, as well as the buckwheat extract, Cassia seed extract and Guaiac extract act synergistically and none of them can be missing.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-acne composition, characterized in that: The invention comprises the following components in parts by weight: 0.01-0.1 parts of saponin A, 0.1-1 parts of saponin B, 5-15 parts of buckwheat extract, 1-10 parts of cassia seed extract, and 1-10 parts of guaiac extract; the saponin A comprises calendulaside and aucubin, the saponin B is sanguisorba officinalis saponin, and the sanguisorba officinalis saponin comprises sanguisorba officinalis saponin I and sanguisorba officinalis saponin II.

2. The anti-acne composition according to claim 1, wherein The invention comprises the following components in parts by weight: 0.05-0.08 parts of saponin A, 0.3-0.5 parts of saponin B, 8-12 parts of buckwheat extract, 6-8 parts of cassia seed extract and 5-8 parts of guaiac extract.

3. The anti-acne composition according to claim 1, wherein In the anti-acne composition, the mass percentage of saponin A is 0.25-0.28%.

4. The anti-acne composition according to claim 1, wherein In the anti-acne composition, the mass percentage of saponin B is 1.3-1.8%.

5. The anti-acne composition according to claim 1, wherein In the anti-acne composition, the total mass percentage of saponin A and saponin B is 1.6-2.1%.

6. The anti-acne composition according to claim 1, wherein The weight ratio of the calendulaside to aucubin is 1:(1.8-4.2); and / or the weight ratio of the sanguisorbaside I to sanguisorbaside II is (11-14):

1.

7. The anti-acne composition according to claim 6, wherein The weight ratio of the aucubin to the aucubin is 1:(2-2.5); and / or the weight ratio of the sanguisorbaside I to the sanguisorbaside II is (12-13):

1.

8. Use of the anti-acne composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the anti-acne composition according to any one of claims 1 to 7.

10. A skin care product, characterized in that: The skin care product comprises the following components in percentage by weight: 1-5% of the anti-acne composition according to any one of claims 1 to 7, 5-40% of a cosmetic base, and the balance water; the cosmetic base comprises at least one of a moisturizer, a thickener, an emulsifier, a skin conditioner, an antioxidant, and an emollient.

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