Application of N-acetyl-L-cysteine in improving drought tolerance of plants

Abstract

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

Description

Technical Field

[0001] The invention relates to the technical field of plant planting, in particular to application of N-acetyl-L-cysteine ​​in improving drought resistance of plants. Background Art

[0002] The intensification of global climate change is one of the most serious challenges facing the world today. The global temperature rise, changes in precipitation patterns and the increase in extreme weather events caused by climate change pose a huge threat to agricultural production. Among them, soil drought is particularly serious, which directly affects the growth of crops, and thus affects food security and sustainable agricultural development. Soil drought refers to the severe lack of water in the soil, which cannot meet the needs of normal plant growth. This situation is becoming increasingly common in many arid and semi-arid regions around the world, especially in the context of climate change, the frequency and intensity of drought events have increased. Crops experience water stress under drought conditions, which can lead to obstruction of physiological and biochemical processes, thereby affecting their growth, development and yield. For example, drought stress usually leads to reduced photosynthesis, stomatal closure and decreased growth rate, which significantly reduces crop yields.

[0003] Brassica napus is an important oil crop widely used in the production of edible oil and industrial raw materials. With the increase of global population and the decrease of resources, it is particularly important to improve the yield and adaptability of rapeseed. Due to the impact of climate change, drought has become one of the main environmental factors limiting the production of Brassica napus. Rapeseed has a relatively high demand for water, and drought will directly affect its growth and development and seed yield. Under drought conditions, the growth of rapeseed plants will be hindered, and the flowering and seed setting periods may be advanced, which usually leads to a significant decrease in yield. In addition, drought may also affect the oil quality and quantity of rapeseed, reducing its economic value. In this context, the development and application of effective drought-resistant technologies and methods have become a research focus.

[0004] N-acetyl-L-cysteine ​​(NAC) is a sulfur-containing amino acid that has important physiological functions in a variety of organisms. Under drought conditions, a large amount of reactive oxygen species will be produced in plants, which will destroy the cell structure and affect the normal physiological functions of plants. N-acetyl-L-cysteine ​​can enhance the antioxidant system of plants, remove excessive reactive oxygen species, and thus reduce the damage of oxidative stress to plants. At present, there are no reports on the use of N-acetyl-L-cysteine ​​to improve plant drought tolerance. Summary of the invention

[0005] The purpose of the present invention is to provide an application of N-acetyl-L-cysteine ​​in improving plant drought tolerance, so as to solve the problems existing in the above-mentioned prior art. N-acetyl-L-cysteine ​​has the ability to improve the resistance of Brassica napus to drought stress. The present invention prepares N-acetyl-L-cysteine ​​into a safe concentration of N-acetyl-L-cysteine ​​solution, which is applied to Brassica napus by foliar spraying. The N-acetyl-L-cysteine ​​solution can significantly improve the fresh weight, dry weight, root length, and chlorophyll content of Brassica napus under drought stress, and reduce the water loss rate of leaves. The present invention is simple to operate, safe to use, and has significant effects, and is beneficial to the tolerance of Brassica napus to drought stress.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] One of the technical solutions of the present invention is the use of N-acetyl-L-cysteine ​​in improving the drought resistance of plants.

[0008] The second technical solution of the present invention is the use of N-acetyl-L-cysteine ​​in the preparation of products for improving plant drought resistance.

[0009] The third technical solution of the present invention is a product for improving plant drought resistance, comprising N-acetyl-L-cysteine.

[0010] A fourth technical solution of the present invention is a method for improving drought resistance of plants, comprising spraying plants with N-acetyl-L-cysteine ​​solution or the product.

[0011] Based on the above technical solution, the present invention has the following technical effects:

[0012] The present invention proposes for the first time the application of N-acetyl-L-cysteine ​​(NAC) in improving the drought resistance of Brassica napus. Experiments have shown that compared with the control group, the rapeseed seedlings sprayed with 150 μM N-acetyl-L-cysteine ​​on the leaves have increased the fresh weight, dry weight, root length, and chlorophyll content of the rapeseed under drought stress conditions, reduced the leaf water loss rate, and enhanced the tolerance of the rapeseed to drought stress. This reflects that the drought stress tolerance of the rapeseed treated with N-acetyl-L-cysteine ​​is significantly improved. Further experimental exploration has obtained that the preferred application concentration is 150 μM, so that N-acetyl-L-cysteine ​​can be optimally utilized in improving the drought stress tolerance of rapeseed. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 The in vivo phenotypes of control and drought-treated Brassica napus after foliar spraying of N-acetyl-L-cysteine ​​solution for 9 days.

[0015] Figure 2 Fresh weight of Brassica napus after foliar spraying of N-acetyl-L-cysteine ​​solution for 9 days and drought treatment.

[0016] Figure 3 The dry weight of Brassica napus after foliar spraying of N-acetyl-L-cysteine ​​solution for 9 days in the control and drought treatments.

[0017] Figure 4 Root length of Brassica napus treated with N-acetyl-L-cysteine ​​solution after foliar spraying for 9 days.

[0018] Figure 5 Chlorophyll content of Brassica napus after foliar spraying of N-acetyl-L-cysteine ​​solution for 9 days and drought treatment.

[0019] Figure 6 Water loss rate of leaves of control and drought-treated Brassica napus after foliar spraying of N-acetyl-L-cysteine ​​solution for 9 days. DETAILED DESCRIPTION

[0020] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0021] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0022] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0023] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present application description and examples are exemplary only.

[0024] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0025] The technical solutions described in the present invention, unless otherwise specified, are all conventional solutions in the art, and the reagents or raw materials used, unless otherwise specified, are purchased from commercial channels or have been disclosed.

[0026] The embodiment of the present invention provides the use of N-acetyl-L-cysteine ​​in improving the drought resistance of plants.

[0027] The chemical structure of N-acetyl-L-cysteine ​​is:

[0028]

[0029] In some specific embodiments, the plant comprises Brassica napus.

[0030] The embodiment of the present invention also provides the use of N-acetyl-L-cysteine ​​in preparing a product for improving plant drought resistance.

[0031] The embodiment of the present invention also provides a product for improving drought resistance of plants, comprising N-acetyl-L-cysteine.

[0032] The embodiment of the present invention also provides a method for improving drought resistance of plants, which comprises spraying the plants with N-acetyl-L-cysteine ​​solution or the product.

[0033] In some specific embodiments, the concentration of the N-acetyl-L-cysteine ​​solution is 150 μM.

[0034] In some specific embodiments, the product is diluted to a concentration of 150 μM N-acetyl-L-cysteine.

[0035] In some specific embodiments, the spraying treatment method is: spraying the leaves of plants during the seedling stage.

[0036] Foliar spraying of 150 μM N-acetyl-L-cysteine ​​solution on Brassica napus increased the fresh weight, dry weight, root length, and chlorophyll content of Brassica napus under drought stress, reduced the leaf water loss rate, and improved the drought stress tolerance of Brassica napus.

[0037] Example 1

[0038] Foliar spraying of N-acetyl-L-cysteine ​​solution

[0039] The seeds of Brassica napus were disinfected by shaking with '84' ​​disinfectant (chlorine content ~ 5%) for 10 minutes, rinsed with double distilled water 3 times, and placed in a culture dish covered with double-layer filter paper for seed germination. After germination for 3 days, the rapeseed with consistent growth was selected and transferred to a hydroponic box for hydroponic growth. The hydroponic solution was Hoagland's nutrient solution, which was changed every 3 days. After continuous replacement for 3 times, 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution was used for foliar spraying of Brassica napus, and the Brassica napus treated with pure water without N-acetyl-L-cysteine ​​was used as a control group. The Hoagland nutrient solution was replaced every 3 days, and different concentrations of N-acetyl-L-cysteine ​​solution were used for foliar spraying.

[0040] Example 2

[0041] PEG6000 drought stress treatment

[0042] The hydroponic solution of the above Brassica napus was replaced with Hoagland's nutrient solution containing 15% PEG6000 to simulate drought stress. The Hoagland's nutrient solution containing 15% PEG6000 was replaced every 3 days. At the same time, the Hoagland's nutrient solution without PEG6000 was used as a control group (Mock) and replaced for 3 consecutive times. Figure 1 As shown, the control group (Mock) was Hoagland's nutrient solution without PEG6000, and the experimental group 15% PEG6000 was used to simulate drought stress treatment.

[0043] The ability of N-acetyl-L-cysteine ​​to enhance the tolerance of Brassica napus to drought stress was comprehensively determined by measuring the fresh weight, dry weight, root length, chlorophyll content and leaf water loss rate of each experimental group and the control group. The fresh weight, dry weight, root length, chlorophyll content and leaf water loss rate of a single plant are five important plant physiological indicators that can reflect the stress resistance level of the plant. Under the same drought treatment conditions, plants that can maintain higher fresh weight, dry weight, root length and chlorophyll content have better drought resistance. The lower the leaf water loss rate, the stronger the water storage capacity of the plant and the better the drought resistance.

[0044] Example 3

[0045] Optimization of N-acetyl-L-cysteine ​​solution concentration

[0046] The materials of the above experimental groups and control groups were examined for data. Take a single plant and use a chlorophyll detector to test the chlorophyll content of the leaves; suck out excess water from the roots and weigh the fresh weight of the single plant, and measure the root length; put the single plant in a 65℃ oven for 24 hours and weigh the dry weight; cut the fresh leaves of the plant and weigh the fresh (M 0 ), and then placed in a constant temperature and humidity treatment room, and the leaf weight (M 3 ), and then use the leaf water loss rate to calculate the leaf water loss rate, that is: leaf water loss rate (%) = (M 0 -M 3 ) / M 0 , the statistical results are shown in Figure 2-6 .

[0047] Depend on Figure 2 It can be found that the fresh weight of the single plant after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution was significantly increased compared with the control group sprayed with pure water under normal conditions, and the fresh weight of the single plant was the largest under the condition of 150 μM. The fresh weight of the single plant after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution was also significantly increased under drought stress conditions compared with the control group sprayed with pure water, and the fresh weight of the single plant was the largest under the condition of 150 μM.

[0048] Depend on Figure 3 It can be found that the dry weight of the single plant after spraying with 150 and 200 μM N-acetyl-L-cysteine ​​solution was significantly increased compared with the control group sprayed with pure water under normal conditions, and the dry weight of the single plant was the largest under the condition of 150 μM. The dry weight of the single plant after spraying with 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution was also significantly increased under drought stress conditions compared with the control group sprayed with pure water, and the dry weight of the single plant was the largest under the condition of 150 μM.

[0049] Depend on Figure 4It can be found that the root length after spraying with 150μM N-acetyl-L-cysteine ​​solution was significantly increased compared with the control group sprayed with pure water under normal conditions, and there was no significant difference compared with the control group. The root length after spraying with 100, 150, 200, and 250μM N-acetyl-L-cysteine ​​solution was significantly increased under drought stress conditions compared with the control group sprayed with pure water, and the root length was the largest under the 150μM condition.

[0050] Depend on Figure 5 It can be found that the chlorophyll content after spraying 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution has no significant difference compared with the control group sprayed with pure water under normal conditions. The chlorophyll content after spraying 100, 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution has no significant difference compared with the control group sprayed with pure water under drought stress conditions, but the average value is the highest under the condition of 150 μM.

[0051] Depend on Figure 6 It can be found that the water loss rate of leaves after spraying with 150, 200, and 250 μM N-acetyl-L-cysteine ​​solution was significantly reduced under normal conditions compared with the control group sprayed with pure water, while the difference was not significant under the 100 μM treatment condition. The water loss rate of leaves after spraying with 100 and 150 μM N-acetyl-L-cysteine ​​solution was significantly reduced under drought stress conditions compared with the control group sprayed with pure water, but the difference was not significant under 200 and 250 μM conditions.

[0052] comprehensive Figure 1 The phenotype and Figure 2-6 The statistical results of physiological index detection showed that after 150μM N-acetyl-L-cysteine ​​solution was sprayed on the leaves of Brassica napus, its fresh weight, dry weight, root length, and chlorophyll content were significantly increased compared with the control, and the leaf water loss rate was significantly reduced compared with the control. All stress resistance physiological indicators were better than the control group and other concentration treatment groups. It can be concluded that the use of 150μM N-acetyl-L-cysteine ​​solution for foliar spraying of Brassica napus can effectively improve the drought stress tolerance of plants, which is of great significance to production practice.

[0053] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For ordinary technical users in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. Application of N-acetyl-L-cysteine ​​in improving plant drought tolerance.

2. The use according to claim 1, characterized in that: The plants include Brassica napus.

3. Application of N-acetyl-L-cysteine ​​in the preparation of products for improving plant drought tolerance.

4. A product for improving drought tolerance of plants, characterized in that: Includes N-acetyl-L-cysteine.

5. A method for improving drought tolerance of plants, characterized in that: The plants are sprayed with N-acetyl-L-cysteine ​​solution or the product according to claim 4.

6. The method according to claim 5, characterized in that The concentration of the N-acetyl-L-cysteine ​​solution was 150 μM.

7. The method according to claim 5, characterized in that The product was diluted so that the concentration of N-acetyl-L-cysteine ​​therein was 150 μM.

8. The method according to claim 5, characterized in that The spraying treatment method comprises: spraying the leaves of plants during the seedling stage.

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