A planting method for relieving drought of wheat based on combined application of SiQD seed dressing and halophilic bacillus

Abstract

The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, and belongs to the technical field of agricultural planting. The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, which comprises the following steps: using silicon quantum dots (SiQDs) for seed dressing treatment of wheat seeds, then planting the seed-dressed wheat seeds in soil, and applying halophilic bacillus agent during the growth of the wheat. The combined application of SiQDs and halophilic bacillus GS127 can better promote the root development of wheat under drought stress, increase the content of proline and soluble sugar in the wheat, reduce the content of malondialdehyde, enhance the drought resistance of the wheat, effectively promote the growth of the wheat, and improve the yield and water use efficiency, and therefore has excellent application potential in the production in agricultural wheat drought areas.

Description

Technical Field

[0001] This invention relates to the field of agricultural planting technology, and more specifically to a planting method for alleviating wheat drought based on the combined application of SiQDs seed dressing and halophilic Bacillus. Background Technology

[0002] Arid regions cover approximately 41% of the global land area, and with climate change, the frequency and intensity of drought stress are intensifying. By 2100, the area affected by drought is projected to expand by 4%-8%, posing a serious threat to global food production. Drought severely threatens agricultural production, particularly during the critical and highly sensitive seed germination stage of the plant life cycle. Water shortage inhibits seed osmosis, thereby reducing seed germination rate and vigor. In later growth stages, drought limits photosynthesis, disrupts osmotic regulation, and significantly reduces biomass accumulation. Furthermore, drought disrupts redox homeostasis, leading to excessive accumulation of reactive oxygen species, which in turn triggers membrane lipid peroxidation and the production of malondialdehyde, ultimately impairing plant growth. Wheat ( Triticum aestivum Wheat (L.) is the world's second-largest cereal crop and is particularly vulnerable to drought, currently affecting approximately 15% of global wheat production. Therefore, developing effective drought mitigation strategies is crucial for ensuring global food security. This research seeks to identify more efficient and environmentally friendly technologies to reduce the negative impacts of drought stress on wheat growth, achieve stable increases in wheat yield, and provide strong support for ensuring food security and sustainable agricultural development in arid regions.

[0003] Silicon (Si) is the second most abundant element in the Earth's crust. It can promote the absorption of water and nutrients by plants, increase the accumulation of osmotic substances such as proline, and enhance antioxidant defense. Silicon-based nanomaterials have been successfully applied as activators in crops such as rice and cucumber, promoting rapid seed germination, improving seedling vigor, and enhancing growth. Silicon quantum dots are a new generation of silicon nanomaterials with a particle size of less than 10 nm. They possess high stability, excellent biocompatibility, and good water solubility. Their ultra-small size allows them to penetrate plant cell walls and enter seeds to exert their effects.

[0004] Bacillus plays a crucial role in the process of plants resisting drought stress. These strains can assist plants in coping with drought stress through various pathways, specifically in the following aspects: (1) They possess functions such as phosphorus solubilization, potassium solubilization, and nitrogen fixation, providing more nutrients to plants in drought-stressed environments and helping them grow. For example, Bacillus can secrete organic acids and phosphatases to convert phosphorus in the soil that is difficult to absorb into a usable form, supplementing key nutrients for plants growing under water-deficient conditions and ensuring the normal development of their basic metabolic activities. (2) They can secrete hormones such as indoleacetic acid (IAA) and cytokinins. Among them, IAA participates in the regulation of plant growth and development, which can promote the growth of plant roots under drought stress, making the roots more developed and extending to a wider range, thereby strengthening the plant's ability to absorb and utilize deep soil water and nutrients, and improving its drought resistance. (3) They can produce 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase. When plants encounter drought stress, they accumulate a large amount of ethylene in their bodies. Excessive ethylene concentration will inhibit plant growth, causing problems such as leaf wilting and slow growth. ACC deaminase can degrade ACC, a precursor for ethylene synthesis, effectively reducing the ethylene content in plants and mitigating the inhibitory effect of drought on plant growth. (4) It can produce extracellular polysaccharides (EPS). EPS can form a protective film around plant roots, reducing soil moisture evaporation and improving soil aggregate structure, enhancing soil water retention capacity. In addition, EPS can adhere to the surface of plant cells, reducing the rate of cell water loss, helping plants maintain normal physiological functions in water-deficient environments, and significantly improving drought resistance. Bacillus can reduce the oxidative damage caused by drought stress to plants and maintain normal plant growth metabolism through the synergistic regulation of the above-mentioned multiple mechanisms. This provides an important solution for coping with drought climate and improving agricultural production in arid areas.

[0005] Therefore, providing a planting method for alleviating wheat drought based on the combined application of SiQDs seed dressing and halophilic Bacillus is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides a planting method for alleviating wheat drought based on the combined application of SiQDs seed dressing and halophilic Bacillus. The combined application of silicon quantum dots and halophilic Bacillus can reduce oxidative damage under drought stress by modulating multiple mechanisms.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A method for alleviating wheat drought based on the combined application of SiQDs seed dressing and halophilic Bacillus includes the following steps:

[0009] (1) Preparation of SiQDs: Under vigorous stirring, 1 mL of 3-aminopropyltrimethoxysilane (APTMS) and 0.8 g of sodium citrate were added to 20 mL of ultrapure water and stirred for 5 min to obtain a mixture. The mixture was transferred to a 100 mL polytetrafluoroethylene-lined stainless steel autoclave and heated at 170 °C for 12 h. After cooling to room temperature, the solution was purified by dialysis (dialysis bag molecular weight = 500 Da) for 24 h, with the water being replaced every 4 h. The purified SiQDs were obtained by vacuum freeze drying.

[0010] (2) SiQDs combined with Bacillus halophilus GS127 alleviated drought stress in wheat:

[0011] Wheat seeds were treated with a 500 mg / L SiQDs solution at a ratio of 1 L SiQDs solution for 5 kg of wheat seeds. The treated wheat seeds were planted in the soil. One month and five months after sowing, a halophilic Bacillus GS127 solution with an OD600 of 0.8 was sprayed at a rate of 10 L per acre each time.

[0012] The halophilic Bacillus ( Bacillus halotolerans GS127, with accession number CCTCC NO: M20252329, is deposited at the China Center for Type Culture Collection (CCTCC), located at Wuhan University, Wuhan, China, on October 24, 2025. It is classified and named as follows: Bacillus halotolerans GS127.

[0013] Furthermore, the method is applied to increase wheat yield under drought conditions.

[0014] Furthermore, the method is applied to improve wheat water use efficiency under drought conditions.

[0015] As can be seen from the above technical solution, compared with the prior art, this invention discloses a planting method for alleviating wheat drought based on the combined application of SiQDs seed treatment and halophilic Bacillus. Silicon quantum dots (SiQDs) are used for seed treatment of wheat seeds, followed by planting the treated wheat seeds in the soil, and halophilic Bacillus inoculant is applied during the wheat growth period. A combined application system of SiQDs and halophilic Bacillus GS127 is constructed, and the effect of the combined application on alleviating wheat drought stress is evaluated through pot experiments. Further field experiments are conducted to verify the application effect. It was found that the combined application of SiQDs and halophilic Bacillus GS127 can better promote wheat root development under drought stress, increase the content of proline and soluble sugars in wheat, reduce malondialdehyde content, enhance wheat drought resistance, effectively promote wheat growth, and improve yield and water use efficiency, showing excellent application potential in agricultural wheat production in arid areas. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 This is a transmission electron microscope image of SiQDs.

[0018] Figure 2 The image shows the Fourier transform infrared spectrum of SiQDs.

[0019] Figure 3 The image shows the X-ray photoelectron spectrum; where A represents the full spectrum of SiQDs; B represents the fine C 1s spectrum of SiQDs; C represents the fine O 1s spectrum of SiQDs; and D represents the fine Si 2p spectrum of SiQDs.

[0020] Figure 4 Photographs of wheat pot experiments treated with SiQDs in combination with Bacillus halophilus GS127.

[0021] Figure 5 The graph shows the results of wheat growth index determination under SiQDs combined with Bacillus halophilus GS127 treatment; where A represents the bar chart of wheat plant height under SiQDs combined with Bacillus halophilus GS127 treatment; B represents the bar chart of fresh weight of wheat underground parts; and C represents the bar chart of fresh weight of wheat aboveground parts.

[0022] Figure 6 This is a scanning image of wheat root morphology after treatment with SiQDs and Bacillus halophilus GS127.

[0023] Figure 7 The figure shows the effect of SiQDs combined with Bacillus halophilus GS127 treatment on wheat root morphology; where A represents the total root length bar chart; B represents the root surface area bar chart; C represents the root volume bar chart; and D represents the root tip number bar chart.

[0024] Figure 8 The graph shows the results of proline content determination in wheat tissues treated with SiQDs in combination with Bacillus halophilus GS127; where A represents the bar chart of proline (Pro) content in wheat stems and leaves; and B represents the bar chart of proline (Pro) content in wheat roots.

[0025] Figure 9The figure shows the results of the determination of soluble sugar content in wheat tissues treated with SiQDs in combination with Bacillus halophilus GS127; where A represents the bar chart of soluble sugar content in wheat stems and leaves; and B represents the bar chart of soluble sugar content in wheat roots.

[0026] Figure 10 The graph shows the results of malondialdehyde (MDA) content determination in wheat tissues treated with SiQDs in combination with Bacillus halophilus GS127; where A represents the bar chart of MDA content in wheat stems and leaves; and B represents the bar chart of MDA content in wheat roots.

[0027] Figure 11 Photographs of wheat pot plants at maturity after treatment with SiQDs in combination with Bacillus halophilus GS127.

[0028] Figure 12 The results of wheat field treatment with SiQDs combined with Bacillus halophilus GS127 are shown in Figure A; A represents the wheat yield bar chart; B represents the wheat water use efficiency bar chart. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] PKO inorganic phosphorus solid medium, Monkina organic phosphorus solid medium, CAS solid medium and Kings liquid medium were all purchased from Haibo Biotechnology Co., Ltd.

[0031] Example 1: Screening of growth-promoting bacteria and characterization of SiQDs synthesis

[0032] (I) Isolation and purification of strains

[0033] Soil samples were collected from dryland areas in Gansu Province. LB liquid medium was prepared, and 5 g of fresh soil samples were cultured in it on a shaker for 1 day for enrichment. The bacterial suspension was serially diluted, spread, and incubated upside down in an incubator for 3-7 days. Colony growth was observed and recorded. Single colonies with different morphologies, sizes, and colors were picked and streaked onto corresponding solid plates for purification. Repeated streaking was performed until a pure bacterial strain was isolated. The culture was then preserved in glycerol and stored at -80°C.

[0034] (ii) Strain identification

[0035] A single colony was picked from a purified bacterial plate that had undergone multiple streaking tests and dissolved in 10 μL of sterile water in a clean bench. One μL of this solution was used as a template for PCR amplification. Universal primers 27F (5′-AGAGTTTGATCCTGGCTC-3′; SEQ ID NO.1) and 1492R (5′-CGGCTACCTTGTTACGACTT-3′; SEQ ID NO.2) were used for PCR amplification. The PCR stock solution was sent to Xi'an Qingke Biotechnology Co., Ltd. for sequencing. The sequencing results are shown in SEQ ID NO.3. The obtained sequences were compared with those on the NCBI website to obtain the species information for each strain.

[0036]

[0037] (III) Screening of functional strains

[0038] 1) Screening of phosphorus-solubilizing and nitrogen-fixing strains

[0039] The glycerol-preserved bacterial strain was inoculated into LB liquid medium for activation. The medium was shaken at 180 r / min and 28℃ until turbidity was observed. The bacterial suspension was then centrifuged at 6000 r / min for 8 min to collect the cells. The cells were washed twice with sterile water, and the OD value of the suspension was adjusted using sterile water. 600 Adjust the pH to approximately 0.8. Add 10 μL of bacterial suspension to PKO inorganic phosphorus solid medium, Monkina organic phosphorus solid medium, and CAS solid medium. Repeat each treatment three times and incubate at 28℃ for 3-7 days. Observe whether there is a clear zone on each solid medium. If a clear zone appears, it proves that the strain has the ability to dissolve inorganic phosphorus, organic phosphorus, and produce siderophores. Record the diameter of the clear zone (D) and the colony diameter (d). Use the ratio of these two diameters (D / d) to determine the extent of the strain's various abilities.

[0040] 2) Screening of EPS-producing strains

[0041] (1) The phenol-sulfuric acid method was used to quantitatively determine the EPS production capacity of the strain. A glucose standard curve was plotted, with glucose concentration on the x-axis and OD... 490 The vertical axis is denoted as y.

[0042] (2) Take the strain frozen in a glycerol tube at -80℃, activate it, dilute it and spread it on LB solid medium plates, incubate it upside down at 28℃ for 3 days, and use an inoculation loop to pick up the colonies to observe whether they are "sticky". If they are sticky, it is preliminarily considered that they may be EPS-producing strains. Incubate the above potential EPS-producing strains in a shaker at 180 r / min and 28℃ for 2 days, centrifuge the bacterial suspension, collect 2 mL of supernatant in a centrifuge tube, add anhydrous ethanol at a ratio of 1:3, and place it in a refrigerator at 4℃ for alcohol precipitation overnight. Repeat each treatment three times. Observe and record the corresponding strains that produce precipitate in the centrifuge tubes. This strain is likely to have the ability to produce EPS. Further centrifuge to collect the precipitate, dry it, and add deionized water to dissolve the precipitate. Add concentrated sulfuric acid and 6% phenol solution in sequence. A yellow reaction occurs. Measure the OD. 490 Based on the glucose standard curve mentioned above, the EPS production capacity of the corresponding strains was determined.

[0043] 3) Screening of IAA-producing strains

[0044] (1) Preparation of IAA solutions with different concentration gradients. 35% HClO4 and 0.5 mol / L FeCl3 were mixed in a 50:1 ratio to obtain the Salkowski colorimetric solution. Equal volumes of IAA solutions with different concentration gradients were mixed with the colorimetric solution, and the mixture was reacted in the dark for 30 min. The OD was then measured.530 Plotting IAA solution concentration on the x-axis, OD... 530 Use the vertical axis to plot the standard curve.

[0045] (2) Take OD 600 The bacterial suspension had a concentration of approximately 0.8. Each bacterial suspension was inoculated into Kings liquid medium at a 1% inoculum volume, with 0.2 g / L tryptophan added, and cultured on a shaker for 3 days. The bacterial suspension was centrifuged to collect the supernatant, which was then mixed with an equal volume of colorimetric solution and reacted in the dark for 30 min. Three replicates were performed for each culture. The mixture was observed to turn pink; if pink, it indicated an IAA-producing strain. The corresponding strains that produced pink were recorded, and the OD was measured. 530 The IAA concentration of the supernatant was obtained.

[0046] 4) Screening of ACC-producing deaminase strains

[0047] (1) Qualitative analysis: Strains that can grow in ADF medium with ACC as the sole nitrogen source are preliminarily determined to have the ability to produce ACC deaminase. Three replicates were performed for each strain. ADF medium formulation: KH2PO4 4 g / L, Na2HPO4 6 g / L, MgSO4 0.2 g / L, glucose 2 g / L, gluconic acid 2 g / L, citric acid 2 g / L, ACC 0.3 g / L, trace element solution 10 mL / L. Trace element solution formulation: KI 0.83 mg / L, H3BO3 6.2 mg / L, MnSO4 22.3 mg / L, ZnSO4 8.6 mg / L, Na2MoO4 0.25 mg / L, CuSO4 0.025 mg / L, CoCl2 0.025 mg / L.

[0048] (2) Quantitative analysis: The amount of α-ketobutyric acid produced per milligram of bacterial protein per hour (µmol) is defined as the activity of the strain in producing ACC deaminase, expressed in U / mL. Strains preliminarily identified as having the ability to produce ACC deaminase were activated in LB liquid medium, and the cells were collected by centrifugation. The cells were washed twice with 0.1 mol / L Tris-HCl (pH 7.5) and resuspended in ADF liquid medium, then cultured on a shaker for 2-3 days. The cells were collected and washed twice again with Tris-HCl (pH 7.5). The obtained cells were mixed with 0.1 mol / L Tris-HCl (pH 8.5) and pure toluene, and ultrasonically disrupted to obtain a crude enzyme solution. The crude enzyme solution was reacted with ACC, and the OD was finally measured. 540 A standard solution of α-ketobutyric acid was prepared, and a standard curve was plotted. Furthermore, the bacterial protein content was determined using the Coomassie Brilliant Blue method, and the ACC deaminase activity of each strain was finally calculated.

[0049] 5) Functional strains

[0050] As shown in Table 1, a total of 1 functional bacteria was screened, and 1 bacterial strain was used for subsequent pot experiments.

[0051] Table 1 Functional strains

[0052]

[0053] Note: "-" indicates that the strain does not have this function.

[0054] According to NCBI comparison, GS127 is a halophilic bacillus. Bacillus halotolerans GS127, with accession number CCTCC NO: M 20252329, is deposited at the China Center for Type Culture Collection (CCTCC), located at Wuhan University, Wuhan, China, on October 24, 2025. It is classified and named as follows: Bacillus halotolerans GS127.

[0055] (iv) Preparation of SiQDs: Under vigorous stirring, 1 mL of 3-aminopropyltrimethoxysilane (APTMS) and 0.8 g of sodium citrate were added to 20 mL of ultrapure water and stirred for 5 min to obtain a mixture. The mixture was transferred to a 100 mL polytetrafluoroethylene-lined stainless steel autoclave and heated at 170 °C for 12 h. After cooling to room temperature, the solution was purified by dialysis (molecular weight = 500 Da) for 24 h, with the water replaced every 4 h. Solid SiQDs were obtained by vacuum freeze-drying.

[0056] SiQDs were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FITR), and X-ray photoelectron spectroscopy (XPS). Results are shown below. Figures 1-3 .

[0057] like Figure 1 As shown, the size of SiQDs in the TEM image is approximately 0.21 nm. Figure 2 FTIR analysis revealed abundant hydrophilic groups on the surface of SiQDs, with characteristic peaks corresponding to Si-N (830 cm⁻¹). -1 ), Si-O (1080 cm) -1 ), CO (1460 cm) -1 C=O (1590 cm) -1 ) and NH / OH (3440 cm -1 ). Figure 3 These are the results of XPS analysis. XPS analysis confirmed the presence of Si, C, O, N, and Na. Figure 3 A). High-resolution C 1s spectra show characteristic peaks for C=C (binding energy: 284.8 eV) and C=O (288.3 eV). Figure 3 B). In the high-resolution O 1s spectrum, peaks corresponding to Si-O (531.8 eV) and C=O (535.7 eV) were observed. Figure 3 C). The Si 2p spectrum also shows a peak value of Si-O (102.4 eV). Figure 3 D).

[0058] Example 2: Pot experiment on the relief of drought stress in wheat by SiQDs combined with Bacillus halophilus GS127

[0059] 1) Experimental plant: Wheat (Taimai 2513). Culture conditions: 16 h light / 8 h dark, temperature 25℃ day / 20℃ night, water artificially controlled. Experimental soil: collected from the Zhongyang Experimental Base in Jinzhong, Shanxi Province.

[0060] (1) Select wheat seeds of uniform size and full size, disinfect them with a 5% NaClO solution, and rinse them three times with distilled water after disinfection. After disinfection, use 500 mg / L SiQDs to treat the seeds at a rate of 1 L of solution to 5 kg of wheat seeds.

[0061] (2) Divide the soil into flower pots, 2 kg per pot, plant wheat seeds in the soil, and adjust the soil moisture by adding tap water to achieve normal moisture content (60% of field capacity) and dry moisture content (40% of field capacity). After one week of growth, thin out the seedlings and keep three seedlings that are growing well and uniform.

[0062] (3) After thinning, bacterial inoculation was carried out. Halophilic Bacillus GS127 was inoculated into LB medium and cultured at 28℃ and 180 r / min for 24 h to obtain the bacterial culture. The OD of the bacterial culture was then measured. 600 Adjust the pH to around 0.8. Inoculate the seedlings with the bacterial solution by root irrigation. The amount of bacterial solution inoculated per seedling is 10 mL, which means the amount of bacterial solution inoculated per pot of plants is 30 mL.

[0063] To verify the effects of SiQDs combined with Bacillus halophilus GS127 on the aboveground and underground parts of wheat plants under drought conditions, five treatment groups were set up: (1) Control: normal treatment group (60% of field capacity); (2) Drought: drought stress (40% of field capacity); (3) Silicon quantum dots: drought stress (40% of field capacity) + SiQDs; (4) Bacillus halophilus: drought stress (40% of field capacity) + Bacillus halophilus GS127; (5) Silicon quantum dots + Bacillus halophilus: drought stress (40% of field capacity) + SiQDs + Bacillus halophilus GS127. Each group had 6 replicates. After 4 weeks, the aboveground and underground parts of wheat plants were collected for relevant index measurements.

[0064] 2) Measurement results:

[0065] The growth indicators (plant height, fresh weight) and root development (total root length, number of root tips, total root volume, and total root surface area) of wheat were measured.

[0066] Wheat growth index measurement results as follows Figure 4 and Figure 5 As shown, under drought stress, the biomass of wheat leaves and roots in the SiQDs combined with Bacillus halophilus GS127 treatment group increased significantly.

[0067] wheat root morphology scans under different treatments are shown below. Figure 6 As shown.

[0068] The results of the measurement of wheat root development indicators are as follows: Figure 7 As shown, under drought stress, treatment with SiQDs combined with Bacillus halophilus GS127 significantly promoted root development and increased total root length, root tip number, total root surface area, and total root volume.

[0069] The contents of proline, soluble sugars, and malondialdehyde were determined using a kit purchased from Beijing Solarbio Science & Technology Co., Ltd. The results are as follows: Figures 8-10 As shown, osmotic regulators, as key factors in plant response to drought stress, play a central role in regulating plant metabolic activities and maintaining osmotic pressure stability. Drought stress leads to a decrease in the activity of antioxidant enzymes in wheat tissues, while wheat can enhance its adaptability to drought environments by accumulating osmotic regulators. Studies have found that the combined application of SiQDs and Bacillus halophilus can effectively reduce the malondialdehyde content in wheat tissues, thereby alleviating lipid peroxidation damage. At the same time, this combined treatment can also promote the synthesis and accumulation of proline and soluble sugars in wheat, ultimately improving the drought resistance of wheat by strengthening the reserve of osmotic regulators.

[0070] Photos of wheat potted plants at maturity treated with SiQDs and Bacillus halophilus GS127 are shown below. Figure 11 As shown, wheat ears are small under drought stress; under the combined treatment, wheat ears are fuller.

[0071] Example 3: Field application of SiQDs combined with Bacillus halophilus GS127 to alleviate drought stress in wheat

[0072] 1) Select uniform-sized, disease- and pest-free wheat seeds (Taimai 2513) and plant them at the Dongyang Experimental Base in Jinzhong City, Shanxi Province, a semi-arid agricultural area. Divide the planting area into 30 m² plots. 2 The area is 3.0 m × 10.0 m, and there is a 0.5 m buffer zone between adjacent areas.

[0073] Four treatment groups were set up in the experiment: (1) Control: no additional fertilizer was applied; (2) Halophilic Bacillus: Halophilic Bacillus GS127 was applied alone (10 L / mu); (3) Silicon Quantum Dots: SiQDs were used for seed dressing; (4) Silicon Quantum Dots + Halophilic Bacillus: Halophilic Bacillus GS127 (10 L / mu) and SiQDs were applied together. SiQDs were used to dress wheat seeds: SiQDs were used at a concentration of 500 mg / L, and 5 kg of wheat seeds were dressed with 1 L of solution. Each treatment was set up with 4 replicates. All treatment groups were sown with the same amount of nitrogen, phosphorus and potassium fertilizer as base fertilizer before planting. Halophilic Bacillus GS127 (OD600 of bacterial solution was 0.8) was applied by spraying one month and five months after wheat sowing, with a dosage of 10 L per mu each time.

[0074] 2) Measurement results:

[0075] The results of wheat field yield and water use efficiency treated with SiQDs combined with Bacillus halophilus GS127 are shown in the figure. Figure 12 See Table 2. Under drought stress, the SiQDs combined with Bacillus halophilus GS127 treatment group significantly increased wheat yield and water use efficiency.

[0076] Table 2

[0077]

[0078] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A planting method for alleviating wheat drought based on the combined application of SiQDs seed dressing and halophilic Bacillus, characterized in that, Includes the following steps: (1) Preparation of SiQDs: Under vigorous stirring, 1 mL of 3-aminopropyltrimethoxysilane and 0.8 g of sodium citrate were added to 20 mL of ultrapure water and stirred for 5 min to obtain a mixture; the mixture was transferred to a 100 mL polytetrafluoroethylene-lined stainless steel autoclave and heated at 170 °C for 12 h; after cooling to room temperature, the solution was purified by dialysis for 24 h, with the water being replaced every 4 h; purified SiQDs were obtained by vacuum freeze drying; the dialysis bag used for dialysis had a molecular weight of 500 Da; (2) SiQDs combined with Bacillus halophilus GS127 alleviated drought stress in wheat: Wheat seeds were treated with a 500 mg / L SiQDs solution at a ratio of 1 L SiQDs solution for 5 kg of wheat seeds. The treated wheat seeds were planted in the soil. One month and five months after sowing, a halophilic Bacillus GS127 solution with an OD600 of 0.8 was sprayed at a rate of 10 L per acre each time. The preservation number of the halophilic Bacillus GS127 is CCTCC NO: M 20252329.

2. The application of the method of claim 1 in increasing wheat yield under drought conditions.

3. The application of the method of claim 1 in improving wheat water use efficiency under drought conditions.

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