Method for low-high temperature stimulation of blastoderm rupture and threshold of tolerance to double high water temperature for schizothoracine fishes in strong diurnal temperature difference water

By using an experimental method that simulates natural water temperature changes on the Qinghai-Tibet Plateau, the critical water temperature for hatching of fertilized eggs of the schizothorax fish and the threshold for tolerance to double high temperatures were determined. This solved the problem of the impact of drastic daily temperature fluctuations on fish hatching, improved the hatching rate and reduced the egg mortality rate, and provided a scientific basis and protection measures.

CN119522858BActive Publication Date: 2026-06-26CHANGJIANG RIVER SCI RES INST CHANGJIANG WATER RESOURCES COMMISSION +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHANGJIANG RIVER SCI RES INST CHANGJIANG WATER RESOURCES COMMISSION
Filing Date
2024-12-31
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing research has failed to effectively investigate the impact of drastic diurnal temperature variations on the development of fertilized eggs in schizothorax fish, especially in climate-sensitive areas such as the Qinghai-Tibet Plateau. This has affected the water temperature tolerance of fish under artificial breeding, thus impacting the effectiveness of stock enhancement and release programs.

Method used

A method for rupture of schizothorax fish in waters with high diurnal temperature range and extreme water temperatures with two high temperatures was designed. By simulating natural water temperature changes indoors and using artificial incubation, combined with low-temperature and high-temperature stimulation, the critical water temperature for rupture and the threshold for tolerance to extreme high temperatures were determined, thus simulating the diurnal water temperature changes in rivers on the Qinghai-Tibet Plateau.

Benefits of technology

This study revealed the water temperature tolerance range and dual high-temperature thresholds for the hatching of fertilized eggs of the small-headed naked carp, improving the hatching rate and reducing the egg mortality rate, and providing scientific evidence and protection measures in the context of climate change.

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Abstract

The application provides a strong diurnal temperature range water area schizothorax ova low temperature-high temperature stimulation membrane rupture and double high water temperature tolerance threshold experiment method, including: step one, determining the diurnal extreme value and amplitude of the hatching water temperature of the schizothorax ova; step two, realizing the diurnal amplitude hatching water temperature; step three, key water temperature control of membrane rupture; step four, low temperature-high temperature stimulation large-scale membrane rupture; step five, determining the double high water temperature tolerance threshold. The application determines the diurnal extreme value and amplitude of the hatching water temperature of the natural reproduction ova, designs different diurnal amplitude hatching water temperature experiment hatching ova, simulates the natural hatching environment of the Qinghai-Tibet Plateau river with large diurnal water temperature difference under climate change; through the indoor ova water temperature gradient experiment, the low temperature-high temperature stimulation membrane rupture mechanism of the Schizothorax grahami, the double high water temperature tolerance range and threshold of the key fish in the source region of the Yangtze River are found, and scientific support is provided for the protection and restoration of the fish resources in the source region of the Yangtze River under the background of climate warming.
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