Traditional Chinese medicine composition with detoxification function as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition with a detoxifying function. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-8 parts of a traditional Chinese medicine extract A, 9-15 parts of a traditional Chinese medicine extract B, 3-11 parts of a traditional Chinese medicine extract C and 7-13 parts of a traditional Chinese medicine extract D, the traditional Chinese medicine extract A comprises the following raw materials: radix angelicae, honeysuckle, fructus forsythiae, dandelion and herba violae; the traditional Chinese medicine extract B is prepared from the following raw materials: fructus gardeniae, radix paeoniae rubra, radix scutellariae, radix et rhizoma rhei, radix trichosanthis, flos lonicerae, radix saposhnikoviae, herba taraxaci, herba violae and fructus forsythiae; the traditional Chinese medicine extract C comprises the following raw materials: akebiaquinata, cicada slough, radix scrophulariae The traditional Chinese medicine extract D comprises the following raw materials: platycodon grandiflorum, liquorice and cortex dictamni. According to the invention, 18 traditional Chinese medicines such as honeysuckle, rhubarb, philippine violet herb, fructus forsythiae, dandelion, cape jasmine, dahurian angelica root and the like are taken as main medicinal materials, are enriched, purified and compatible, can be used for effectively treating diseases caused by sore and furuncle ulceration, scorching fever and purulent running water, and have the advantages of obvious drug effect, high bioavailability and stable and reliable quality.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition with detoxification function, a preparation method and application thereof. Background Art

[0002] The existing Lianqiao Baidu Tablets have been included in the Ministry-issued Chinese Medicine Formula Preparations (Volume 19). Its process and technical characteristics are as follows: Platycodon grandiflorum, Angelica dahurica, Radix Trichosanthis, Rhubarb, and Fritillaria thunbergii are crushed into fine powders respectively and sieved; Viola yedoensis, Taraxacum officinale, Scrophularia ningpoensis, Forsythia suspensa, Licorice, Gardenia jasminoides, Periostracum cicadae, and Flos lonicerae are decocted with water, the decoction is filtered, and the filtrate is concentrated into a thick paste; Saposhnikovia divaricata, Cortex Dictamni, Paeonia lactiflora, and Rhizoma Cicadae are infiltrated with ethanol as a solvent; ethanol is recovered from the slurry and concentrated into a thick paste; the thick pastes obtained above are combined, fine powders such as Platycodon grandiflorum and auxiliary materials are added, mixed evenly, made into granules, dried, and pressed into tablets.

[0003] The main disadvantages of the current technology are that the process is relatively backward, the types of chemical components extracted are relatively small, the content of effective ingredients is low, and the dosage required is large.

[0004] Therefore, it is very necessary to provide a Chinese medicine composition with detoxification function that can effectively treat the symptoms caused by boils, furuncles, burning fever, pus and water discharge. Summary of the invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a Chinese medicine composition with detoxification function. The Chinese medicine composition provided by the present invention has a good detoxification effect.

[0006] The invention uses 18 kinds of Chinese medicinal materials such as honeysuckle, rhubarb, Viola yedoensis, forsythia, dandelion, gardenia, and angelica as main medicinal materials, and after enrichment, purification, and compatibility, it can effectively treat diseases caused by sores, boils, ulcers, burning fever, pus and water discharge.

[0007] The Chinese medicine composition prepared by the present invention has significant efficacy, high bioavailability, stable and reliable quality, and can be prepared into different dosage forms according to the needs of patients. The Chinese medicine composition is convenient to carry and easy to take. At the same time, the new prescription has a unique taste and is suitable for a wide range of people.

[0008] In the prescription, honeysuckle clears heat and detoxifies, dispels wind-heat, forsythia clears heat and detoxifies, dispels wind-heat, relieves swelling and resolves knots, violet yedoensis clears heat and detoxifies, cools blood and relieves swelling, dandelion clears heat and detoxifies, eliminates carbuncle and resolves knots, and relieves dampness and relieves stranguria. The four medicines are monarch medicines, mainly clearing heat and detoxifying; gardenia cools blood and detoxifies, rhubarb clears heat and detoxifies, scutellaria purges fire and detoxifies, thunbergia thunbergii resolves knots and relieves swelling, scrophularia resolves toxins and resolves knots, and white mulberry bark clears heat and detoxifies, and assists the monarch medicine in detoxifying, and is the minister medicine; platycodon discharges pus, akebia relieves heat, saposhnikovia root overcomes dampness and relieves pain, red peony root clears heat and relieves pain, angelica root relieves swelling and discharges pus, cicada shell clears rash and relieves itching, and radix trichosanthis relieves swelling and discharges pus, and is used for carbuncle, sore and toxic, and is a total of adjuvant medicines; liquorice detoxifies and harmonizes the various medicines, and is the guiding medicine. The combination of the various medicines has the effects of clearing heat and detoxifying, eliminating carbuncle and discharging pus, and achieves the function of detoxification.

[0009] The detoxification described in the present invention refers to the ability to effectively treat symptoms caused by boils, ulcers, burning fever, and pus and water discharge.

[0010] The present invention provides a traditional Chinese medicine composition with detoxification function, comprising the following raw materials in parts by weight:

[0011] 2-8 parts of Chinese herbal medicine extract A, 9-15 parts of Chinese herbal medicine extract B, 3-11 parts of Chinese herbal medicine extract C, and 7-13 parts of Chinese herbal medicine extract D;

[0012] The raw materials of the Chinese medicine extract A include Angelica dahurica, honeysuckle, forsythia, dandelion and Viola yedoensis; the raw materials of the Chinese medicine extract B include Gardenia jasminoides, red peony root, scutellaria baicalensis, rhubarb, Radix Trichosanthis, honeysuckle, Saposhnikovia divaricata, dandelion, Viola yedoensis and forsythia suspensa; the raw materials of the Chinese medicine extract C include Akebia trifoliata, Periostracum cicadae, Scrophularia ningpoensis and Fritillaria thunbergii; the raw materials of the Chinese medicine extract D include Platycodon grandiflorum, Licorice and Dictamni cortex.

[0013] According to the present invention, the detoxifying Chinese medicine composition comprises the following raw materials in parts by weight:

[0014] 3-7 parts of Chinese herbal medicine extract A, 9-13 parts of Chinese herbal medicine extract B, 3-9 parts of Chinese herbal medicine extract C, and 8-11 parts of Chinese herbal medicine extract D.

[0015] In some preferred embodiments of the present invention, the detoxifying Chinese medicine composition comprises the following raw materials in parts by weight:

[0016] 4-6 parts of Chinese herbal medicine extract A, 9-12 parts of Chinese herbal medicine extract B, 3-8 parts of Chinese herbal medicine extract C, and 9-10 parts of Chinese herbal medicine extract D.

[0017] In one preferred embodiment of the present invention, the detoxifying Chinese medicine composition comprises the following raw materials in parts by weight:

[0018] 5 parts of Chinese herbal medicine extract A, 9 parts of Chinese herbal medicine extract B, 3 parts of Chinese herbal medicine extract C, and 10 parts of Chinese herbal medicine extract D.

[0019] The mass ratio of angelica dahurica, honeysuckle, forsythia suspensa, dandelion and Viola yedoensis in the Chinese medicine extract A of the present invention is (4-6): (5-7): (7-9): (4-6): (5-7);

[0020] In one preferred embodiment of the present invention, the mass ratio of Angelica dahurica, Flos Lonicerae, Fructus Forsythiae, Herba Taraxaci and Viola yedoensis in the Chinese medicine extract A is 5:6:8:5:6.

[0021] The preparation method of the Chinese medicine extract A of the present invention comprises:

[0022] Angelica dahurica, honeysuckle, forsythia, dandelion, Viola yedoensis and water are mixed and extracted by steam distillation to obtain volatile oil; the volatile oil is included in β-cyclodextrin to obtain the product.

[0023] The ratio of the total amount of Angelica dahurica, honeysuckle, forsythia, dandelion and Viola yedoensis to water is preferably 1: (10-12), and more preferably 1: 10. The extraction time of steam distillation is 2-3 hours, preferably 3 hours.

[0024] The volatile oil is collected, 4 to 5 times the amount of β-cyclodextrin and 1 to 2 times the amount of water are added, and the mixture is stirred at a constant speed for 1 to 2 hours using a magnetic stirrer, and the inclusion mixture is passed through a colloid mill 1 to 3 times to evaporate the liquid to obtain extract A. More preferably, the volatile oil is collected, 4 times the amount of β-cyclodextrin and 1 times the amount of water are added, and the mixture is stirred at a constant speed for 1 hour using a magnetic stirrer, and the inclusion mixture is passed through a colloid mill 2 times to evaporate the liquid to obtain extract A.

[0025] According to the present invention, the mass ratio of Gardenia jasminoides, Paeonia lactiflora, Scutellaria baicalensis, Rhubarb, Radix Trichosanthis, Flos Lonicerae, Saposhnikoviae, Herba Taraxaci, Viola yedoensis and Fructus Forsythiae in the Chinese medicine extract B is (6-8): (4-6): (1-3): (3-5): (3-5): (3-5): (2-4): (4-6): (7-9): (5-7);

[0026] In one preferred embodiment of the present invention, the mass ratio of Gardenia, Red Peony Root, Scutellaria, Rhubarb, Radix Trichosanthis, Flos Lonicerae, Saposhnikoviae, Taraxaci, Viola yedoensis and Fructus Forsythiae of the Chinese medicine extract B is 7:5:2:4:4:4:3:5:8:6.

[0027] Wherein, the preparation method of the Chinese medicine extract B comprises:

[0028] S1) Gardenia jasminoides, red peony root, scutellaria baicalensis, rhubarb, root of trichosanthes and honeysuckle are mixed, flash extracted with 30% ethanol, filtered, and concentrated under reduced pressure to obtain powder a;

[0029] S2) mixing siler, dandelion, Viola yedoensis and forsythia, adding cellobiase, pectinase and 50% ethanol, adjusting the pH value to 3.5, shaking in an air bath, sonicating, concentrating and freeze-drying to obtain powder b;

[0030] S3) Mixing the powder a and the powder b to obtain extract B.

[0031] The preparation method of the Chinese medicine extract B of the present invention is as follows: first, Gardenia, Red Peony Root, Scutellaria baicalensis, Rhubarb, Radix Trichosanthis and Flos Lonicerae are crushed, preferably to 80 meshes. Flash extraction is performed using 30% ethanol, the material-liquid ratio is 1:50 g / mL, and the extraction time is 3 to 5 minutes, preferably, the extraction time is 3 minutes. Filter, concentrate the filtrate under reduced pressure, and freeze-dry the concentrate to obtain powder a.

[0032] Saposhnikovia divaricata, dandelion, Viola yedoensis and Forsythia suspensa are mixed and crushed, preferably to 80 meshes. Cellobiase, pectinase and 50% ethanol are added. The amount of cellobiase and pectinase added is 50 mg each.

[0033] The total amount of the siler, dandelion, violet yedoensis and forsythia suspensa to 50% ethanol has a solid-liquid ratio of 1:8. The air bath shaking is preferably: constant temperature air bath shaking at 50°C for 1 hour. Then cool to room temperature, ultrasonic at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrated solution is freeze-dried to obtain powder b.

[0034] The drug powder a and the drug powder b are mixed to obtain extract B.

[0035] The mass ratio of Akebia, Periostracum Cicadae, Scrophulariae and Fritillariae Thunbergii in the Chinese medicine extract C of the present invention is (5-7): (3-5): (3-5): (5-7);

[0036] In a preferred embodiment, the mass ratio of Akebia, Periostracum Cicadae, Scrophularia and Fritillaria thunbergii is 6:4:4:6.

[0037] According to the present invention, the preparation method of the Chinese medicine extract C comprises:

[0038] i) Akebia, Periostracum Cicadae and Scrophulariae are extracted by microwave using 60% ethanol, and the extract is centrifuged, filtered and freeze-dried to obtain powder c;

[0039] ii) crushing Fritillaria thunbergii to obtain medicinal powder d;

[0040] iii) Mixing drug powder c and drug powder d to obtain extract C.

[0041] The preparation method of the traditional Chinese medicine extract C provided by the invention comprises the following steps: firstly, akebia, cicada shell and scrophularia are crushed, passed through a 60-mesh sieve, and extracted by a microwave extraction method; the ethanol concentration of the microwave extraction is 60%; the solid-liquid ratio is 1:20-30 (g / mL), the microwave power is 250-265W, the processing time is 80-90s, the extraction is carried out in a constant temperature shaking incubator for 1h, the extract is placed in a refrigerated centrifuge at 10000r / min for 10-15min, filtered, ethanol is recovered, and the concentrated liquid is freeze-dried to obtain the medicine powder C.

[0042] Preferably, the solid-liquid ratio is 1:20 (g / mL), the microwave power is 259W, the processing time is 90s, the extraction is carried out in a constant temperature shaking incubator for 1h, the mixture is placed in a refrigerated centrifuge at 10000r / min for 10min, filtered, ethanol is recovered, and the concentrated solution is freeze-dried to obtain drug powder c.

[0043] The Fritillaria thunbergii was crushed and passed through a 120-mesh sieve to obtain powder d. Powder c was mixed with powder d to obtain extract C.

[0044] The mass ratio of Platycodon grandiflorum, Licorice root and Dictamni root in the Chinese medicine extract D of the present invention is (3-5): (4-6): (2-4);

[0045] In a specific embodiment, the mass ratio of Platycodon grandiflorum, Licorice and Dictamni Cortex is 4:5:3.

[0046] According to the present invention, the preparation method of the Chinese medicine extract D comprises:

[0047] Platycodon grandiflorum, liquorice and Dictamni cortex were mixed and extracted with supercritical CO2 to obtain extract D.

[0048] Preferably, Platycodon grandiflorum, liquorice and Dictamni cortex are mixed, crushed, passed through a 60-mesh sieve, and extracted by supercritical CO2; the parameters of the supercritical CO2 extraction include: extraction pressure of 25-35MPa, extraction temperature of 40-50°C, and extraction time of 100-120min; preferably, the parameters of the supercritical CO2 extraction include: extraction pressure of 30MPa, extraction temperature of 45°C, and extraction time of 120min.

[0049] The present invention provides a method for preparing a detoxifying Chinese medicine composition according to any one of the above technical solutions, comprising the following steps:

[0050] The Chinese herbal medicine extract A, the Chinese herbal medicine extract B, the Chinese herbal medicine extract C and the Chinese herbal medicine extract D are mixed to obtain the product.

[0051] The specific preparation methods of the above-mentioned Chinese herbal extract A, Chinese herbal extract B, Chinese herbal extract C, and Chinese herbal extract D have been clearly described above and will not be repeated here.

[0052] The present invention does not limit the above-mentioned mixing method, and any mixing method known to those skilled in the art may be used.

[0053] The present invention provides the use of the traditional Chinese medicine composition described in the above technical solution in preparing a drug with detoxification function.

[0054] The Chinese medicine composition provided by the present invention can significantly reduce the content of TNF-α and IL-6 in the lung and serum; the body weight is significantly reduced (P<0.05), the lung weight is increased (P<0.05), and the organ index is significantly increased (P<0.01).

[0055] The present invention provides a drug with detoxification function, comprising the Chinese medicinal composition with detoxification function described in any one of the above technical solutions.

[0056] The present invention also includes auxiliary materials or ingredients commonly used in the pharmaceutical field.

[0057] The dosage form of the present invention includes, but is not limited to, one or more of granules, decoctions, powders, oral liquids, tablets, pills, pastes or capsules.

[0058] The invention provides a Chinese medicine composition with detoxification function, comprising the following raw materials in parts by weight: 2-8 parts of Chinese medicine extract A, 9-15 parts of Chinese medicine extract B, 3-11 parts of Chinese medicine extract C, and 7-13 parts of Chinese medicine extract D; the raw materials of the Chinese medicine extract A include angelica dahurica, honeysuckle, forsythia, dandelion and Viola yedoensis; the raw materials of the Chinese medicine extract B include gardenia, red peony root, scutellaria baicalensis, rhubarb, root of trichosanthes, honeysuckle, siler, dandelion, Viola yedoensis and forsythia; the raw materials of the Chinese medicine extract C include akebia, cicada shell, Scrophularia ningpoensis and Fritillaria thunbergii; the raw materials of the Chinese medicine extract D include platycodon, liquorice and Dictamni cortex. The invention uses 18 kinds of traditional Chinese medicines such as honeysuckle, rhubarb, viola yedoensis, forsythia, dandelion, gardenia, angelica dahurica and the like as main medicinal materials, which are enriched, purified and combined to effectively treat diseases caused by ulcers, furuncles, burning fever, pus and water discharge, and has significant medicinal effect, high bioavailability and stable and reliable quality. DETAILED DESCRIPTION

[0059] The present invention provides a Chinese medicine composition with detoxification function and its preparation method and application. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they all belong to the scope of protection of the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the method and application of this article without departing from the content, spirit and scope of the present invention to realize and apply the technology of the present invention.

[0060] In this application, the term "and / or" describes the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. A and B can be singular or plural.

[0061] In this application, "at least one" means one or more, "more than one" means two or more. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items.

[0062] It should be understood that in the various embodiments of the present application, the size of the serial numbers of the above-mentioned processes does not mean the order of execution, some or all of the steps can be executed in parallel or sequentially, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0063] The numerical ranges and parameters involved in the present invention have presented the relevant numerical values ​​in the specific embodiments as accurately as possible. However, any numerical value essentially inevitably contains standard deviations due to individual testing methods. Therefore, unless otherwise explicitly stated, it should be understood that all numerical ranges or specific data used in this disclosure may have certain reasonable deviations within a certain range, for example: within 1% or 0.5%.

[0064] Some cases are recorded in the embodiments and comparative examples of the present invention, wherein the embodiments show certain implementation modes of the present invention. However, this does not mean that the effects of the present invention can only be achieved in these cases.

[0065] To further illustrate the present invention, a Chinese medicine composition with detoxification function provided by the present invention, its preparation method and application are described in detail below in conjunction with examples.

[0066] Example 1

[0067] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0068] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0069] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0070] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0071] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0072] Take powder a and powder b and mix them evenly to obtain extract B.

[0073] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0074] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0075] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0076] Take powder c and powder d and mix them evenly to obtain extract C.

[0077] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0078] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0079] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0080] Take 2 parts of a Chinese herbal extract A with detoxification function, 15 parts of a Chinese herbal extract B, 3 parts of a Chinese herbal extract C, and 7 parts of a Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0081] Example 2

[0082] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0083] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0084] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0085] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0086] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0087] Take powder a and powder b and mix them evenly to obtain extract B.

[0088] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0089] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0090] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0091] Take powder c and powder d and mix them evenly to obtain extract C.

[0092] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0093] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0094] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0095] Take 2 parts of a Chinese herbal extract A with detoxification function, 12 parts of a Chinese herbal extract B, 7 parts of a Chinese herbal extract C, and 10 parts of a Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0096] Example 3

[0097] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0098] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0099] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0100] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0101] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0102] Take powder a and powder b and mix them evenly to obtain extract B.

[0103] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0104] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0105] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0106] Take powder c and powder d and mix them evenly to obtain extract C.

[0107] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0108] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0109] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0110] Take 2 parts of a Chinese herbal extract A with detoxification function, 9 parts of Chinese herbal extract B, 11 parts of Chinese herbal extract C, and 13 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0111] Example 4

[0112] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0113] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0114] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0115] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0116] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0117] Take powder a and powder b and mix them evenly to obtain extract B.

[0118] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0119] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0120] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0121] Take powder c and powder d and mix them evenly to obtain extract C.

[0122] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0123] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0124] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0125] Take 5 parts of a Chinese herbal extract A with detoxification function, 15 parts of a Chinese herbal extract B, 7 parts of a Chinese herbal extract C, and 13 parts of a Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0126] Example 5

[0127] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0128] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0129] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0130] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0131] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0132] Take powder a and powder b and mix them evenly to obtain extract B.

[0133] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0134] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0135] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0136] Take powder c and powder d and mix them evenly to obtain extract C.

[0137] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0138] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0139] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0140] Take 5 parts of a Chinese herbal extract A with detoxification function, 12 parts of Chinese herbal extract B, 11 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0141] Example 6

[0142] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0143] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0144] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0145] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0146] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0147] Take powder a and powder b and mix them evenly to obtain extract B.

[0148] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0149] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0150] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0151] Take powder c and powder d and mix them evenly to obtain extract C.

[0152] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0153] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0154] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0155] Take 5 parts of a Chinese herbal extract A with detoxification function, 9 parts of Chinese herbal extract B, 3 parts of Chinese herbal extract C, and 10 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0156] Example 7

[0157] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0158] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0159] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0160] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0161] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0162] Take powder a and powder b and mix them evenly to obtain extract B.

[0163] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0164] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0165] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0166] Take powder c and powder d and mix them evenly to obtain extract C.

[0167] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0168] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0169] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0170] Take 8 parts of a Chinese herbal extract A with detoxification function, 15 parts of Chinese herbal extract B, 11 parts of Chinese herbal extract C, and 10 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0171] Example 8

[0172] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0173] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0174] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0175] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0176] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0177] Take powder a and powder b and mix them evenly to obtain extract B.

[0178] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0179] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0180] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0181] Take powder c and powder d and mix them evenly to obtain extract C.

[0182] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0183] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0184] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0185] Take 8 parts of a Chinese herbal extract A with detoxification function, 12 parts of a Chinese herbal extract B, 3 parts of a Chinese herbal extract C, and 13 parts of a Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0186] Example 9

[0187] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0188] Take 5 parts of Angelica dahurica, 6 parts of honeysuckle, 8 parts of forsythia, 5 parts of dandelion and 6 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0189] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0190] 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria Baicalensis, 4 parts of Rhubarb, 4 parts of Radix Trichosanthis, and 4 parts of Flos Lonicerae are ground and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is concentrated under reduced pressure. The concentrate is freeze-dried to obtain powder a.

[0191] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, 8 parts of Viola yedoensis, and 6 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0192] Take powder a and powder b and mix them evenly to obtain extract B.

[0193] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0194] 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, a processing time of 90 s, and extraction was performed in a constant temperature shaking incubator for 1 h. The mixture was centrifuged in a refrigerated centrifuge at 10,000 r / min for 10 min, filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0195] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0196] Take powder c and powder d and mix them evenly to obtain extract C.

[0197] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0198] Take 4 parts of Platycodon grandiflorum, 5 parts of Licorice, and 3 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45℃, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0199] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0200] Take 8 parts of a Chinese herbal extract A with detoxification function, 9 parts of Chinese herbal extract B, 7 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0201] Comparative Example 1

[0202] The difference from Example 6 is that 5 parts of Angelica dahurica, 3 parts of Honeysuckle and 4 parts of Forsythia suspensa were taken in Extract A, decocted in water twice, 10 times the amount of water was added for the first time, and extracted for 3 hours, and 8 times the amount of water was added for the second time, and extracted for 2 hours. The two water extracts were combined, filtered, and the filtrate was concentrated under reduced pressure to a clear paste with a relative density of 1.15, dried under reduced pressure, and the dry paste was crushed to obtain powder A.

[0203] Comparative Example 2

[0204] The difference from Example 6 is that 7 parts of Gardenia, 5 parts of Red Peony Root, 2 parts of Scutellaria baicalensis, 8 parts of Rhubarb, and 8 parts of Radix Trichosanthis are taken from Extract B, the medicinal materials are crushed, passed through an 80-mesh sieve, 8 times the amount of 40% ethanol is added, and after ultrasonic extraction for 20 minutes, the mixture is filtered, the filtrate is concentrated under reduced pressure, and the concentrate is freeze-dried to obtain powder a.

[0205] Take 3 parts of Saposhnikovia divaricata, 5 parts of Taraxacum mongolicum, and 6 parts of Viola yedoensis, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover the ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0206] Take powder a and powder b and mix them evenly to obtain extract B.

[0207] Comparative Example 3

[0208] The difference from Example 6 is that 6 parts of Akebia, 4 parts of Periostracum Cicadae, and 4 parts of Scrophularia ningpoensis were taken from Extract C, decocted with water twice, 10 times the amount of water was added for the first time, and extracted for 3 hours, and 8 times the amount of water was added for the second time, and extracted for 2 hours. The two water extracts were combined, filtered, and the filtrate was concentrated under reduced pressure to a clear paste with a relative density of 1.15, dried under reduced pressure, and the dry paste was crushed to obtain powder C.

[0209] Take 6 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0210] Take powder c and powder d and mix them evenly to obtain extract C.

[0211] Comparative Example 4

[0212] The difference from Example 6 is that 4 parts of Platycodon grandiflorum and 5 parts of Licorice are taken in Extract D, crushed, passed through a 60-mesh sieve, added with 95% ethanol, and ultrasonically extracted for 30 minutes, filtered, ethanol recovered, the extract concentrated, and the concentrate freeze-dried to obtain Extract D.

[0213] Comparative Example 5

[0214] Different from Example 6,

[0215] 1. A method for preparing a Chinese herbal extract A having a detoxifying function:

[0216] Take 5 parts of Angelica dahurica, 4 parts of honeysuckle, 4 parts of forsythia, 3 parts of dandelion and 3 parts of Viola yedoensis, add them into 10 times of water, extract by steam distillation for 3 hours, collect volatile oil, add 4 times of β-cyclodextrin and 1 times of water, stir at a constant speed with a magnetic stirrer for 1 hour, pass the included mixture through a colloid mill twice, evaporate the liquid to dryness, and obtain extract A.

[0217] 2. A method for preparing a Chinese herbal extract B with detoxification function:

[0218] 5 parts of Gardenia, 5 parts of Red Peony Root, 5 parts of Scutellaria Baicalensis, 7 parts of Rhubarb, 3 parts of Radix Trichosanthis, and 3 parts of Flos Lonicerae are crushed and passed through an 80-mesh sieve. Flash extraction is performed, 30% ethanol is added, the solid-liquid ratio is 1:50 g / mL, the extraction time is 3 minutes, and the filtrate is filtered, the filtrate is concentrated under reduced pressure, and the concentrate is freeze-dried to obtain powder a.

[0219] Take 5 parts of Saposhnikovia divaricata, 2 parts of Taraxacum mongolicum, 2 parts of Viola yedoensis, and 3 parts of Forsythia suspensa, grind them, pass through an 80-mesh sieve, add cellobiase and pectinase, add 8 times the amount of 50% ethanol, adjust the pH to 3.5, shake in a constant temperature air bath at 50°C for 1 hour, cool to room temperature, ultrasonicate at 35°C for 20 minutes, recover the ethanol, and concentrate under reduced pressure to a thick paste with a relative density of 1.25-1.30. The concentrate is freeze-dried to obtain powder b.

[0220] Take powder a and powder b and mix them evenly to obtain extract B.

[0221] 3. A method for preparing a Chinese herbal extract C with detoxification function:

[0222] 5 parts of Akebia, 3 parts of Periostracum Cicadae, and 5 parts of Scrophularia were crushed and passed through a 60-mesh sieve. Microwave extraction was performed with an ethanol concentration of 60%, a solid-liquid ratio of 1:20 (g / mL), a microwave power of 259 W, and a processing time of 90 s. The extraction was carried out in a constant temperature shaking incubator for 1 h. The extraction was carried out in a refrigerated centrifuge at 10,000 r / min for 10 min. The mixture was filtered, ethanol was recovered, and the concentrated solution was freeze-dried to obtain a powder a.

[0223] Take 5 parts of Fritillaria thunbergii, grind at low temperature, and pass through a 120-mesh sieve to obtain powder d.

[0224] Take powder c and powder d and mix them evenly to obtain extract C.

[0225] 4. A method for preparing a Chinese herbal extract D having a detoxifying function:

[0226] Take 5 parts of Platycodon grandiflorum, 5 parts of Licorice, and 5 parts of Dictamni Cortex, grind the three Chinese medicines through 60 mesh, use supercritical CO2 extraction technology, the extraction pressure is 30MPa, the extraction temperature is 45°C, 95% ethanol is added as an entrainer, the extraction time is 120 minutes, and extract D is obtained.

[0227] 5. Preparation of a Chinese herbal extract composition with detoxification function

[0228] Take 5 parts of a Chinese herbal extract A with detoxification function, 9 parts of Chinese herbal extract B, 3 parts of Chinese herbal extract C, and 10 parts of Chinese herbal extract D, mix them evenly, add an appropriate amount of maltodextrin, and make 1000 tablets.

[0229] Comparative Example 6

[0230] Different from Example 6, 4 parts of Platycodon grandiflorum, 5 parts of Angelica dahurica, 4 parts of Radix Trichosanthis, 4 parts of Radix Rhei, 6 parts of Fritillariae Thunbergii are taken, and they are crushed into fine powder respectively and sieved; 14 parts of Viola yedoensis, 10 parts of Herba Taraxaci, 4 parts of Scrophulariae, 14 parts of Fructus Forsythiae, 5 parts of Licorice, 7 parts of Gardenia, 6 parts of Akebia, 4 parts of Periostracum Cicadae, 10 parts of Flos Lonicerae are decocted twice with water, each time for 2 hours, and the decoction is combined, filtered, and the filtrate is concentrated into a paste. 3 parts of Saposhnikovia divaricata, 3 parts of Dictamni, 5 parts of Radix Paeoniae Rubra, 2 parts of Scutellariae baicalensis are used as solvent, 1975ml of 60% ethanol is used for percolation, and ethanol is recovered from the percolation liquid and concentrated into a paste. The water and alcohol concentrated extracts are combined, dried, crushed into fine powder, and fine powders such as 4 parts of Platycodon grandiflorum, corn starch, and dextrin are added in appropriate amounts, mixed, made into granules, dried, and magnesium stearate is added in appropriate amounts, pressed into 1000 tablets, and film-coated to obtain.

[0231] Validation Example 13: Comparative Experimental Study on the Pharmacodynamics of Extracts

[0232] method

[0233] 1. Mouse grouping, modeling and drug administration

[0234] ICR mice were taken in half male and half female, with a body weight of 18-22 g, and randomly divided into a blank group, a model group, a Chinese medicine positive group (Lianqiao Baidu Tablets, a dose of 25 g / kg), a Western medicine positive group (dexamethasone, a dose of 5 mg / kg), an administration group I (Example 1), an administration group II (Example 2), an administration group III (Example 3), an administration group IV (Example 4), an administration group V (Example 5), an administration group VI (Example 6), an administration group VII (Example 7), an administration group VIII (Example 8), an administration group IX (Example 9), an administration group X (Comparative Example 1), an administration group XI (Comparative Example 2), an administration group XII (Comparative Example 3), an administration group XIII (Comparative Example 4), an administration group XIV (Comparative Example 5), and an administration group XV (Comparative Example 6), with 10 mice in each group and an administration dose of 20 g / kg. The dexamethasone group was intraperitoneally injected once on days 2, 5, and 7, and the other groups were intragastrically administered once a day for 7 consecutive days. 30 minutes after the last administration, lipopolysaccharide (LPS, 5 mg / kg) was intraperitoneally injected into the mice in all groups except the blank group to prepare an acute lung injury model. 6 hours after LPS injection, blood was collected from the mice to separate serum, and lung tissue was dissected for detection of relevant indicators.

[0235] 2. Detection of mouse body weight and organ index

[0236] The changes in body weight and appearance of rats in each group during modeling and drug administration were observed and recorded, such as coat color, motor function, whether metabolites are normal, and whether there are abnormal secretions, etc., to evaluate the changes in the general state of the animals. The body weight of each group of mice was measured before dissection, and the mice were killed by cervical dislocation after blood was taken from the eyeballs. The complete lungs were immediately removed and placed in a physiological saline solution to rinse the blood. The filter paper was dried to absorb water and measure the lung mass, i.e., the lung mass index. Lung coefficient (%) = lung mass index / body weight * 100%.

[0237] 3. Detection of TNF-α and IL-6 levels in mouse serum and lung tissue

[0238] The collected blood samples and lung tissues were processed accordingly and stored in a refrigerator at -20°C. The TNF-α and IL-6 contents were detected according to the instructions of the TNF-α and IL-6 kits.

[0239] 4. Statistical Analysis

[0240] Excel statistical analysis was used for data analysis. Data were expressed as x ± s, and one-way analysis of variance was used to compare the sample means among multiple groups. P < 0.05, P < 0.01, P < 0.001 were considered statistically significant.

[0241] result

[0242] 1. General status of mice in each group

[0243] The mice in the blank group ate and drank normally, moved freely, breathed steadily, had shiny fur, normal appearance, and increased body weight rapidly. As the experiment progressed, the mice in the model group showed reduced food intake, discomfort, reduced activity, rapid breathing, and partial hair loss. The mice in the Chinese medicine positive group, Western medicine positive group, medication group Ⅰ, medication group Ⅱ, medication group Ⅲ, medication group Ⅳ, medication group Ⅴ, medication group Ⅵ, medication group VII, medication group VIII, medication group IX, medication group Ⅹ, medication group XI, medication group XII, medication group XIII, medication group XIV, and medication group XV also had the above symptoms, but the onset time was later and the degree was milder. Among them, the Western medicine positive group, medication group VI, and medication group VII were better than other medication groups.

[0244] 2. Body weight and organ index of mice in each group

[0245] Compared with the blank group, the body weight of the model group was significantly reduced (P<0.01), the lung weight was increased, and the organ index was significantly increased (P<0.05); compared with the model group, the body weight of the western medicine positive group, medication group VI, and group VII was significantly reduced (P<0.05), the lung weight was increased (P<0.05), and the organ index was significantly increased (P<0.01). The other medication groups all changed, but there was no significant significance (P>0.05); compared with the Chinese medicine positive group, the body weight of the medication group VI was reduced, the lung weight was increased, and the organ index was increased. From the overall data analysis, the medication group VI was better than Lianqiao Baidu Tablets (Chinese medicine positive group) and other medication groups, see Table 1.

[0246] Table 1. Body weight, lung weight and organ index of mice in each group (`x±s, n=10)

[0247]

[0248]

[0249] Note: Compared with the blank group, #P<0.05, ##P<0.01; compared with the model group, *P<0.05, **P<0.01; compared with the Chinese medicine positive group, +P<0.05, ++P<0.01.

[0250] 3. TNF-α and IL-6 levels in mouse serum

[0251] Compared with the blank group, the levels of TNF-α and IL-6 in the serum of mice in the model group were significantly increased (P<0.001); compared with the model group, the levels of TNF-α and IL-6 in the western medicine positive group were significantly decreased (P<0.001, P<0.05), the levels of TNF-α and IL-6 in the medication group VI were significantly decreased (P<0.01, P<0.01), and the levels of TNF-α and IL-6 in the other medication groups were decreased; compared with the Chinese medicine positive group, the levels in the medication group VI were significantly decreased (P<0.05); from the analysis of the overall data of the levels of TNF-α and IL-6 in the serum of mice in each group, the medication group VI was better than Lianqiao Baidu Tablets (Chinese medicine positive group) and other medication groups, see Table 2.

[0252] Table 2. TNF-α and IL-6 levels in the serum of mice in each group (`x±s, n=10)

[0253]

[0254]

[0255] Note: Compared with the blank group, ###P<0.001; compared with the model group, *P<0.05, **P<0.01, ***P<0.001; compared with the Chinese medicine positive group, +P<0.05, ++P<0.01.

[0256] 4. TNF-α and IL-6 levels in mouse lung tissue

[0257] Compared with the blank group, the TNF-α and IL-6 contents in the lung tissue of the model group mice were significantly increased (P<0.001); compared with the model group, the TNF-α and IL-6 contents in the western medicine positive group were significantly decreased (P<0.01, P<0.05), the TNF-α and IL-6 contents in the VI group were significantly decreased (P<0.01, P<0.05), and the TNF-α and IL-6 contents in the other medication groups were decreased; compared with the Chinese medicine positive group, the TNF-α and IL-6 contents in the VI group were significantly decreased (P<0.05, P<0.05). From the analysis of the overall data on the TNF-α and IL-6 contents in the lung tissue of mice in each group, the VI group was better than Lianqiao Baidu Tablets (the Chinese medicine positive group) and the other medication groups, as shown in Table 3.

[0258] Table 3. TNF-α and IL-6 levels in lung tissues of mice in each group (`x±s, n=10)

[0259]

[0260]

[0261] Note: Compared with the blank group, ###P<0.001; compared with the model group, *P<0.05, **P<0.01; compared with the Chinese medicine positive group, +P<0.05, ++P<0.01.

[0262] According to the above data, the Chinese medicine composition VI of the present invention has a comparable effect to the western medicine positive control group, and is superior to the original preparation of Lianqiao Baidu Tablets under the same dosage condition (P<0.05), and is superior to other drug administration groups.

[0263] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese medicine composition with detoxification function, characterized in that: The invention comprises the following raw materials in parts by weight: 2-8 parts of Chinese herbal medicine extract A, 9-15 parts of Chinese herbal medicine extract B, 3-11 parts of Chinese herbal medicine extract C, and 7-13 parts of Chinese herbal medicine extract D; The raw materials of the Chinese medicine extract A include Angelica dahurica, honeysuckle, forsythia, dandelion and Viola yedoensis; the raw materials of the Chinese medicine extract B include Gardenia jasminoides, red peony root, scutellaria baicalensis, rhubarb, Radix Trichosanthis, honeysuckle, Saposhnikovia divaricata, dandelion, Viola yedoensis and forsythia suspensa; the raw materials of the Chinese medicine extract C include Akebia trifoliata, Periostracum cicadae, Scrophularia ningpoensis and Fritillaria thunbergii; the raw materials of the Chinese medicine extract D include Platycodon grandiflorum, Licorice and Dictamni cortex.

2. The Chinese medicine composition according to claim 1, characterized in that: The detoxifying Chinese medicine composition comprises the following raw materials in parts by weight: 3-7 parts of Chinese herbal medicine extract A, 9-13 parts of Chinese herbal medicine extract B, 3-9 parts of Chinese herbal medicine extract C, and 8-11 parts of Chinese herbal medicine extract D.

3. The Chinese medicine composition according to claim 1 or 2, characterized in that: The mass ratio of angelica dahurica, honeysuckle, forsythia suspensa, dandelion and Viola yedoensis in the Chinese medicine extract A is (4-6): (5-7): (7-9): (4-6): (5-7); The preparation method of the Chinese medicine extract A comprises: mixing angelica dahurica, honeysuckle, forsythia, dandelion, Viola yedoensis and water, extracting by steam distillation to obtain volatile oil; and encapsulating the volatile oil with beta-cyclodextrin to obtain the extract.

4. The Chinese medicine composition according to claim 1 or 2, characterized in that: The mass ratio of Gardenia jasminoides, Paeonia lactiflora, Scutellaria baicalensis, Rhubarb, Radix Trichosanthis, Flos Lonicerae, Saposhnikoviae Saposhnikoviae, Herba Taraxaci, Viola yedoensis and Fructus Forsythiae in the Chinese medicine extract B is (6-8): (4-6): (1-3): (3-5): (3-5): (3-5): (2-4): (4-6): (7-9): (5-7); The preparation method of the Chinese medicine extract B comprises: S1) Gardenia jasminoides, red peony root, scutellaria baicalensis, rhubarb, root of trichosanthes and honeysuckle are mixed, flash extracted with 30% ethanol, filtered, and concentrated under reduced pressure to obtain powder a; S2) mixing siler, dandelion, Viola yedoensis and forsythia, adding cellobiase, pectinase and 50% ethanol, adjusting the pH value to 3.5, shaking in an air bath, sonicating, concentrating and freeze-drying to obtain powder b; S3) Mixing the powder a and the powder b to obtain extract B.

5. The Chinese medicine composition according to claim 1 or 2, characterized in that: The mass ratio of Akebia, Periostracum Cicadae, Scrophulariae and Fritillariae Thunbergii in the Chinese medicine extract C is (5-7): (3-5): (3-5): (5-7); The preparation method of the Chinese medicine extract C comprises: i) Akebia, Periostracum Cicadae and Scrophulariae are extracted by microwave using 60% ethanol, and the extract is centrifuged, filtered and freeze-dried to obtain powder c; ii) crushing Fritillaria thunbergii to obtain medicinal powder d; iii) Mixing drug powder c and drug powder d to obtain extract C.

6. The Chinese medicine composition according to claim 1 or 2, characterized in that: The mass ratio of Platycodon grandiflorum, Licorice root and Dictamni root in the Chinese medicine extract D is (3-5): (4-6): (2-4); The preparation method of the Chinese medicine extract D comprises: Platycodon grandiflorum, liquorice and Dictamni cortex were mixed and extracted with supercritical CO2 to obtain extract D.

7. The Chinese medicine composition according to claim 6, characterized in that: The parameters of the supercritical CO2 extraction include: extraction pressure of 30 MPa, extraction temperature of 45°C, and extraction time of 120 min.

8. A method for preparing the detoxifying Chinese medicine composition according to any one of claims 1 to 7, characterized in that: The steps include: The Chinese herbal medicine extract A, the Chinese herbal medicine extract B, the Chinese herbal medicine extract C and the Chinese herbal medicine extract D are mixed to obtain the product.

9. Use of the Chinese medicine composition according to any one of claims 1 to 7 in the preparation of drugs with detoxification function.

10. A drug with detoxification function, characterized in that: A Chinese medicinal composition with detoxification function comprising any one of claims 1 to 7.

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