Process for preparing ginseng freeze-dried powder by combining ginseng medicinal material superfine powder with compound enzyme and application of ginseng freeze-dried powder

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a process for preparing ginseng freeze-dried powder by combining ginseng medicinal material superfine powder with a compound enzyme and application of the ginseng freeze-dried powder. According to the process for extracting ginsenoside by combining superfine powder with composite enzymolysis, plant cell walls of ginseng medicinal materials are broken by fully crushing the medicinal materials, cell dissociation of root tip cells of cell nucleuses is accelerated, the dissolution rate of the ginseng medicinal materials is increased, and then the ginseng medicinal material superfine powder is subjected to enzymolysis treatment by adopting proper enzyme combination and enzymolysis modes, so that the ginsenoside is obtained. The precipitation rate of active ingredients in the ginseng medicinal material is increased, the anti-heart failure activity of the ginseng is effectively improved, the utilization rate of the ginseng medicinal material is increased, the treatment effect on H9c2 heart failure cells is achieved, and the problem of urgent ginseng medicinal material resources is solved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and specifically relates to a process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with composite enzyme and its application. Background Art

[0002] Panax ginseng Panax ginseng The dried root and rhizome of CAMey. were first recorded in Shennong's Herbal Classic. They are top-grade products with a sweet and slightly cold taste. They have the effects of nourishing the internal organs, stopping palpitations, improving eyesight and calming the mind. They are mainly used in the treatment of palpitations, insomnia, heart failure, and low immunity. The main chemical components of ginseng are ginsenosides, volatile oils, ginseng polysaccharides, amino acids, peptides and proteins, among which ginsenosides are the main active ingredients for anti-heart failure.

[0003] Nowadays, the methods for extracting the active ingredients of ginseng are complex and varied, and most of them use organic acid solution treatment or add auxiliary agents to increase the ginseng extraction rate. For example, the patent with application number 202210803694.7 discloses a "method for extracting ginsenosides". Its invention uses an acidic solvent to treat ginseng medicinal materials, and then adds magnetic nanoparticles and / or activated white clay for treatment to obtain ginsenosides. In addition, enzyme treatment methods for ginseng have also appeared one after another. For example, the patent with application number 201611247879.5 discloses "a ginseng hydrolysate and its preparation process and application". Its invention is to first obtain a ginseng extract, and then enzymatically treat the ginseng extract to obtain a ginseng hydrolysate with a specific saponin component.

[0004] However, the ginseng extracts obtained by the above prior art have not fundamentally solved the problem of medicinal material dissolution, and cannot fully obtain the active ingredients in the ginseng medicinal material. The present invention uses high-energy nano-impact grinding of ginseng medicinal materials to obtain ginseng medicinal material ultrafine powder, and then according to the composition of plant cells, we use composite enzymes to crack the plant cell structure to increase the precipitation rate of active ingredients and expand the medicinal utilization rate of ginseng medicinal materials. Summary of the invention

[0005] In view of the above technical problems, the present invention proposes a process and application of preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme. The ginsenoside content in the ginseng freeze-dried powder prepared by the present invention is increased, thereby improving the pharmacological effect of ginseng in resisting heart failure.

[0006] In order to achieve the above object, the technical solution of the present invention is as follows.

[0007] A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme comprises the following steps: Step 1, pulverizing ginseng medicinal material powder by high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder; Step 2: add an appropriate amount of water solvent to the ginseng medicinal material ultrafine powder, adjust the pH value to be weakly acidic, add a certain proportion of complex enzyme for enzymolysis, reflux extraction, cooling, concentrating, and freeze-drying to obtain ginseng freeze-dried powder after enzymolysis.

[0008] Furthermore, in step 1, the ginseng medicinal powder is obtained by drying ginseng to a constant weight, crushing it, and passing it through a 60-mesh sieve to obtain fine powder.

[0009] Furthermore, in step 1, the ultrafine ginseng medicinal powder is crushed by a CJM-SY-B high-energy nano impact mill, and the grinding time is 1 to 3 hours.

[0010] Furthermore, in step 2, the mass ratio of the ginseng medicinal material ultrafine powder to the aqueous solvent is 1:20 to 1:40.

[0011] Furthermore, in step 2, the mass amount of the added complex enzyme is 2% to 4% of the mass of the ginseng medicinal material powder.

[0012] Furthermore, in step 2, the complex enzyme is one of cellulase-meltase, cellulase-pectinase, and pectinase-meltase.

[0013] Furthermore, in step 2, the mass ratio of the two enzymes in the complex enzyme is 1:1 to 2:1.

[0014] Furthermore, in step 2, the enzymatic hydrolysis pH is 6 to 7, the enzymatic hydrolysis temperature is 40° C. to 60° C., and the enzymatic hydrolysis time is 2 to 4 hours.

[0015] Furthermore, in step 2, the reflux extraction time is 2 hours.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows.

[0017] 1. The ginseng freeze-dried powder prepared by the present invention has a main anti-heart failure active ingredient, the sum of ginsenosides Rg1, Re, Rb and Rg2, which far exceeds that of water-extracted ginseng and alcohol-extracted ginseng. At the same time, the ginseng freeze-dried powder prepared by the present invention has a good therapeutic effect on H9c2 heart failure cells, and plays an anti-heart failure activity role by maintaining the normal state of the heart.

[0018] 2. The present invention adopts the process of extracting ginsenosides by combining ultrafine powder with composite enzymatic hydrolysis, and cracks the plant cell wall of ginseng medicinal materials and accelerates the dissociation of cell nuclei and root tip cells by fully crushing the medicinal materials, thereby increasing the dissolution rate of ginseng medicinal materials. Then, a suitable enzyme combination and enzymatic hydrolysis method are used to enzymatically hydrolyze the ultrafine ginseng medicinal materials, thereby increasing the precipitation rate of active ingredients in ginseng medicinal materials, effectively improving the anti-heart failure activity of ginseng, increasing the utilization rate of ginseng medicinal materials, having a therapeutic effect on H9c2 heart failure cells, and solving the urgent problem of ginseng medicinal materials resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The ultrafine powder of ginseng medicinal material produced by high-energy nano-impact mill with different grinding times.

[0020] Figure 2 Single factor results graph.

[0021] Figure 3 Response surface plot. DETAILED DESCRIPTION

[0022] Embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. In the following examples, unless otherwise specified, the experimental methods and technical means used are conventional methods and means in the art. Unless otherwise specified, the remaining reagents and consumables used are purchased from conventional reagent manufacturers in the art.

[0023] A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme comprises the following steps: Step 1, after drying to constant weight, ginseng is crushed, and the fine powder passing through a 60-mesh sieve is crushed by a CJM-SY-B high-energy nano impact mill for 1 to 3 h to obtain ginseng medicinal material ultrafine powder; Step 2, add an appropriate amount of water solvent to the ginseng medicinal material ultrafine powder, adjust the pH value to weak acidity, add a certain proportion of complex enzyme for enzymolysis, the enzymolysis pH is 6-7, the enzymolysis temperature is 40°C-60°C, and the enzymolysis time is 2-4 hours; reflux extraction for 2 hours, cooling, concentrating, and freeze-drying to obtain the ginseng freeze-dried powder after enzymolysis.

[0024] The mass ratio of ginseng medicinal material ultrafine powder to water solvent is 1:20 to 1:40; The added amount of compound enzyme is 2% to 4% of the mass of ginseng medicinal powder; The complex enzyme is one of cellulase-meltase, cellulase-pectinase and pectinase-meltase.

[0025] Example 1.

[0026] A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme comprises the following steps: After drying to constant weight, ginseng was crushed and passed through a 60-mesh sieve to obtain ginseng medicinal powder; the ginseng medicinal powder was then ground using a CJM-SY-B high-energy nano-impact mill for 2 h to obtain ginseng medicinal ultrafine powder; 30 times the weight of ginseng medicinal ultrafine powder was added with water, cellulose and collapsing enzyme (1:1) were added in an amount of 3% of the ginseng medicinal ultrafine powder, the enzymatic hydrolysis temperature was controlled at 50°C, the enzymatic hydrolysis pH was 6.5, the enzymatic hydrolysis time was controlled at 3 h, the extraction was heated under reflux for 2 h, and the extract was cooled. After the filtrate was obtained by vacuum filtration, the excess solvent was removed by a rotary evaporator, and finally freeze-dried into powder to obtain ginseng freeze-dried powder.

[0027] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 58.68%.

[0028] Example 2.

[0029] A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme comprises the following steps: After drying to constant weight, ginseng was crushed and passed through a 60-mesh sieve. It was then ground for 2 h using a CJM-SY-B high-energy nano-impact mill to obtain ginseng ultrafine powder. 30 times the weight of the medicinal material was added with water. Cellulose and pectinase (1:1) were selected and added in an amount of 3% of the medicinal material. The enzymatic hydrolysis temperature was controlled at 50 °C, the enzymatic hydrolysis pH was 6.5, the enzymatic hydrolysis time was controlled at 3 h, and the extraction was heated under reflux for 2 h. The extract was cooled, and the filtrate was obtained by vacuum filtration. The excess solvent was removed by a rotary evaporator, and finally freeze-dried into powder to obtain ginseng freeze-dried powder.

[0030] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 53.37%.

[0031] Example 3.

[0032] A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme comprises the following steps: After drying to constant weight, ginseng was crushed, passed through a 60-mesh sieve, and then ground for 2 hours using a CJM-SY-B high-energy nano impact mill to obtain ginseng ultrafine powder. 30 times the weight of the medicinal material was added with water, pectinase and collapsing enzyme (1:1) were selected, and the addition amount was 3% of the medicinal material. The enzymatic hydrolysis temperature was controlled at 50°C, the enzymatic hydrolysis pH was 6.5, the enzymatic hydrolysis time was controlled at 3 hours, and the extraction was heated and refluxed for 2 hours. After cooling, the extract was vacuum filtered to obtain the filtrate, and the excess solvent was removed by a rotary evaporator. Finally, it was freeze-dried into powder to obtain ginseng freeze-dried powder.

[0033] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 50.37%.

[0034] Comparative example 1.

[0035] A preparation method for a water-extracted ginseng dry powder: after drying the ginseng to a constant weight, the ginseng is crushed and passed through a 60-mesh sieve, 30 times the weight of water of the medicinal material is added, heated and refluxed for 2 hours, cooled, the extract is vacuum filtered to obtain a filtrate, excess solvent is removed by a rotary evaporator, and finally freeze-dried into powder.

[0036] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 30.67%.

[0037] Comparative example 2.

[0038] Preparation of alcohol-extracted ginseng dry powder: After drying to constant weight, ginseng is crushed and passed through a 60-mesh sieve, 70% ethanol 30 times the weight of the medicinal material is added, heated and refluxed for 2 hours, cooled, the extract is vacuum filtered to obtain a filtrate, and excess solvent is removed by a rotary evaporator, and finally freeze-dried into powder.

[0039] Ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 35.20%.

[0040] Comparative example 3.

[0041] A preparation method for water-extracted ginseng medicinal material ultrafine powder: after drying to constant weight, ginseng is crushed, passed through a 60-mesh sieve, and then ground for 2 hours using a CJM-SY-B high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder; 30 times the weight of the medicinal material is added with water, heated and refluxed for 2 hours, cooled, and the extract is vacuum filtered to obtain a filtrate, and then excess solvent is removed using a rotary evaporator, and finally freeze-dried into powder.

[0042] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 36.67%.

[0043] Comparative example 4.

[0044] Preparation of alcohol-extracted ginseng medicinal material ultrafine powder: after drying to constant weight, ginseng is crushed, passed through a 60-mesh sieve, and then ground for 2 hours using a CJM-SY-B high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder; 70% ethanol 30 times the weight of the medicinal material is added, heated to reflux for 2 hours, cooled, and the extract is vacuum filtered to obtain a filtrate, and then excess solvent is removed using a rotary evaporator, and finally freeze-dried into powder.

[0045] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 39.20%.

[0046] Comparative Example 5.

[0047] Preparation of single enzymatic hydrolysis ginseng dry powder: After drying to constant weight, ginseng is crushed, passed through a 60-mesh sieve, and then ground for 2 hours using a CJM-SY-B high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder. Add 30 times the weight of the medicinal material with water, select cellulase, the addition amount is 3% of the medicinal material, the enzymatic hydrolysis temperature is controlled at 50 ° C, the enzymatic hydrolysis pH is 6.5, the enzymatic hydrolysis time is controlled at 3 hours, heat reflux extraction for 2 hours, cool, and the extract is vacuum filtered to obtain the filtrate, and then the excess solvent is removed by a rotary evaporator, and finally freeze-dried into powder to obtain ginseng freeze-dried powder.

[0048] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%, the same below) is 50.31%.

[0049] Comparative Example 6.

[0050] Preparation of ginseng dry powder by single enzymatic hydrolysis invention: After drying to constant weight, ginseng is crushed, passed through a 60-mesh sieve, and then ground for 2 h using a CJM-SY-B high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder. Add 30 times the weight of the medicinal material with water, select pectinase, and add 3% of the medicinal material. The enzymatic hydrolysis temperature is controlled at 50°C, the enzymatic hydrolysis pH is 6.5, the enzymatic hydrolysis time is controlled at 3 h, and the extraction is heated and refluxed for 2 h, cooled, and the extract is vacuum filtered to obtain a filtrate, and then the excess solvent is removed by a rotary evaporator, and finally freeze-dried into powder to obtain ginseng freeze-dried powder.

[0051] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 47.87%.

[0052] Comparative Example 7.

[0053] Preparation of ginseng dry powder by single enzymatic hydrolysis: After drying to constant weight, ginseng is crushed, passed through a 60-mesh sieve, and then ground for 2 h using a CJM-SY-B high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder. Add 30 times the weight of the medicinal material with water, select a decomposing enzyme, and add 3% of the medicinal material. The enzymatic hydrolysis temperature is controlled at 50 ° C, the enzymatic hydrolysis pH is 6.5, the enzymatic hydrolysis time is controlled at 3 h, and the extraction is heated and refluxed for 2 h. Cool, and the extract is vacuum filtered to obtain a filtrate. The excess solvent is removed by a rotary evaporator, and finally freeze-dried into powder to obtain ginseng freeze-dried powder.

[0054] The ginseng paste yield (ginseng freeze-dried powder / ginseng raw drug amount × 100%) is 43.73%.

[0055] test: 1. Determination of saponin content of ginseng freeze-dried powder of the present invention The contents of ginsenosides Rg1, Re, Rb1, Rg2 and the total saponins of the ginseng medicinal materials of Examples 1-3 and Comparative Examples 1-7 were detected, and the results are shown in Table 1.

[0056] Table 1 Ginsenoside content of enzymatic hydrolysates of Examples 1-3 and Comparative Examples 1-7 (mg•g -1 ).

[0057] Note: * Compared with the comparative example 1, P <0.05.

[0058] From the above comparison results, it can be seen that in the method of the present invention, the ginseng medicinal material superfine powder is enzymatically hydrolyzed using a composite enzyme composed of cellulase and catalytic enzyme. The ginseng medicinal material superfine powder can increase the dissolution rate of ginseng active ingredients, and the composite enzyme can fully decompose the cell wall, cell nucleus and other structures of the ginseng plant. The combination of the two can not only increase the ginseng medicinal material paste rate, but also increase the content of saponin components in the ginseng medicinal material.

[0059] 2. Experimental application of ginseng freeze-dried powder in the treatment of heart failure Experimental methods: An in vitro heart failure cell model was constructed. H9c2 rat cardiomyocytes were placed at 37°C, 5% CO2, saturated humidity, and in a sterile environment. The culture medium was replaced every 2-3 days, and cells in the logarithmic growth phase were used for heart failure modeling (doxorubicin). After each group was given the same concentration of ginseng sample intervention, CCK8 staining was performed for 2-4 h, and the absorbance (OD) was measured at a wavelength of 450nm using an enzyme reader. Six replicate wells were set up in each group, and the experiment was repeated 3 times. Cell survival rate = (OD ginseng treatment group - OD blank group) / (OD control group - OD blank group).

[0060] Experimental results: The test results were analyzed using statistics. The statistical analysis software was Origin 2022. Variance analysis was used to compare multiple groups of quantitative data, as shown in Table 2.

[0061] Table 2 Survival rate of H9c2 rat heart failure cells (%).

[0062] Note: * Compared with the comparative example 2, P <0.05.

[0063] The results show that Examples 1 to 3 and Comparative Examples 1 to 7 all have therapeutic effects on H9c2 rat heart failure cells, and Examples 1 to 3 are superior to Comparative Examples 1 to 7. This result shows that the superfine powder in Examples 1 to 3 combined with the ginseng extract under composite enzymatic hydrolysis can enhance the anti-heart failure effect of ginseng medicinal materials, among which Example 1 has the best effect.

[0064] 3. Single factor study on freeze-dried ginseng powder of the present invention Experimental method: Ginseng medicinal materials were taken, crushed through a 60-mesh sieve, and then ground using a CJM-SY-B high-energy nano impact mill (1-3 h). The complex enzyme composed of cellulase and crashase in Example 1 was selected, and the solid-liquid ratio (1:10, 1:20, 1:30, 1:40, 1:50 g / ml), the amount of complex enzyme added (1.0%, 2.0%, 3.0%, 4.0%, 5.0%), the ratio of complex enzyme (1:2, 1:1.5, 1:1, 1.5:1, 2:1), enzymolysis temperature (30, 40, 50, 60, 70°C), enzymolysis pH (5.5, 6.0, 6.5, 7.0, 7.5), and enzymolysis time (1, 2, 3, 4, 5h) were used as conditions. One of the factors was changed, while the other factors remained unchanged, and the effects of these factors on the ginseng extract yield and the survival rate of heart failure cells were investigated.

[0065] Experimental results: Ginseng was ground for 1 to 3 h using CJM-SY-B high-energy nano impact mill. According to the electron microscope images of ginseng, the particle size of ginseng powder ground by high-energy nano impact mill was much smaller than that of powder ground by 60-mesh sieve by pulverizer, and the particle size of ginseng ground by high-energy nano impact mill for 2 h was not much different from that of ginseng ground by 3 h. Therefore, considering the need to save time while reducing the particle size of ginseng, high-energy nano impact mill was the best choice for grinding for 2 h. Figure 1 According to the results of the single factor experiment, the optimal material-liquid ratio, compound enzyme addition amount, compound enzyme ratio, enzymatic hydrolysis temperature, enzymatic hydrolysis pH and enzymatic hydrolysis time were selected. The results are shown in Figure 2 .

[0066] 4. Response surface optimization experiment of ginseng freeze-dried powder of the present invention Experimental method: According to the results of single-factor experiment, a four-factor three-level response surface optimization experiment was designed with the addition amount of complex enzyme (2%, 3%, 4%), enzymatic hydrolysis temperature (40℃, 50℃, 60℃), enzymatic hydrolysis pH (6.0, 6.5, 7.0), and enzymatic hydrolysis time (2h, 3h, 4h). The ointment yield and heart failure cell survival rate were used as evaluation indicators, see Table 3.

[0067] Experimental results: Based on the single factor experiment, Design-Expert 8.0.6 software was used to optimize the four-factor three-level response surface experiment with the addition amount of compound enzyme, enzymatic hydrolysis temperature, enzymatic hydrolysis pH, and enzymatic hydrolysis time. The design is shown in Table 4, and the fitting regression equation is: Y =93.94-1.42 A -0.94 B -0.93 C -0.90 D +0.38 AB +1.09 AC +0.090 AD -0.081 BC +0.039 BD -0.079 CD -8.45 A 2 -5.22 B 2 -4.35 C 2 -1.93 D 2 ; Where: Y is the survival rate of heart failure cells; A It is the amount of two-dimensional plus compound enzyme added; B is the enzymatic hydrolysis temperature; C is the enzymatic pH; D is the enzymatic hydrolysis time; the response surface diagram and the corresponding contour diagram are shown in Figure 3 .

[0068] The final optimal conditions are: 2-4% compound enzyme addition, 40-60°C enzymatic hydrolysis temperature, 6-7 pH, and 2-4 h enzymatic hydrolysis time.

[0069] Table 3. Factor level table of response surface experiment.

[0070] Table 4 Response surface experimental design and results.

[0071] Table 5 Significance test and variance analysis of the regression equation model.

[0072] Note: *Significant difference (0.01< P <0.05); **The difference is extremely significant ( P <0.01); the difference was not significant ( P >0.05).

[0073] The present invention obtains the optimal enzymolysis conditions through single factor and response surface experiments, cellulase: scissile enzyme=1.5:1, compound enzyme addition amount 2.91%, enzymolysis temperature 49.05 DEG C, enzymolysis pH 6.44, enzymolysis time 2.77 h, and combines actual experimental operation to correct the experimental operation, and finally determines that the optimal process for improving the content of ginsenosides is as follows: ginseng medicinal material powder is passed through a 60-mesh sieve, then passed through a high-energy nano impact mill, ground for 2 h, 30 times of aqueous solution is added, cellulase-scissile enzyme is used as a combined compound enzyme, the compound enzyme ratio is 1.5:1, the compound enzyme addition amount is 3%, the enzymolysis temperature is 50 DEG C, the enzymolysis pH is 6.5, and the enzymolysis time is 2.8 h.

[0074] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme, characterized in that: The steps include: Step 1, pulverizing ginseng medicinal material powder by high-energy nano impact mill to obtain ginseng medicinal material ultrafine powder; Step 2: add an appropriate amount of water solvent to the ginseng medicinal material ultrafine powder, adjust the pH value to be weakly acidic, add a certain proportion of complex enzyme for enzymolysis, reflux extraction, cooling, concentrating, and freeze-drying to obtain ginseng freeze-dried powder after enzymolysis.

2. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 1, the ginseng medicinal powder is ginseng that has been dried to a constant weight, crushed, and passed through a 60-mesh sieve to obtain fine powder.

3. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 1, the ultrafine ginseng powder is ground by a CJM-SY-B high-energy nano impact mill for 1 to 3 hours.

4. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 2, the mass ratio of the ginseng medicinal material ultrafine powder to the aqueous solvent is 1:20 to 1:

40.

5. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 2, the mass amount of the added complex enzyme is 2% to 4% of the mass of the ginseng medicinal material powder.

6. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 2, the complex enzyme is one of cellulase-meltase, cellulase-pectinase, and pectinase-meltase.

7. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In step 2, the mass ratio of the two enzymes in the complex enzyme is 1:1 to 2:

1.

8. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 2, the enzymatic hydrolysis pH is 6 to 7, the enzymatic hydrolysis temperature is 40° C. to 60° C., and the enzymatic hydrolysis time is 2 to 4 hours.

9. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to claim 1, characterized in that: In the step 2, the reflux extraction time is 2 hours.

10. The process for preparing ginseng freeze-dried powder by combining ginseng medicinal material ultrafine powder with complex enzyme according to any one of claims 1 to 9, characterized in that: The invention relates to an application of the ginseng freeze-dried powder in preparing an anti-heart failure active drug.

Images