Glomus thunglandii AMG16 for repairing heavy metal contaminated soil and application of glomus thunglandii AMG16

Abstract

The invention relates to glomus thunbergii AMG16 for repairing heavy metal contaminated soil and application of the glomus thunbergii AMG16, the classification name of the glomus thunbergii AMG16 is Glomus caledonium, the preservation number of the glomus thunbergii AMG16 is CGMCC (China General Microbiological Culture Collection Center) NO.41628, and the preservation time of the glomus thunbergii AMG16 is November 15, 2024. The strain is separated from heavy metal contaminated soil, and can help plants to absorb mineral nutrient elements, enhance the composite heavy metal resistance of the plants, further change the absorption, transportation and distribution of the plants to the composite heavy metals, and slow down the composite heavy metal pollution of the soil. As an indigenous strain, the AMG16 strain can effectively cope with complex soil, organisms, climate and various adversity stress coexisting environments in the natural environment, and the activity of the strain after the fungicide is used is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial application, and particularly relates to a Scotch spherical mold AMG16 for repairing heavy metal contaminated soil and an application thereof. Background Art

[0002] In recent years, with the rapid development of industry and agriculture and the use of a large number of pesticides containing complex heavy metals, the complex heavy metal pollution of farmland soil has continued to intensify. The complex heavy metals in the soil not only lead to soil degradation, reduce the quality and yield of crops, but also lead to a decrease in the biomass of soil microorganisms, affect the microbial population structure and reduce the diversity of soil microorganisms, which poses a huge safety hazard to food safety and human health. The remediation of soil complex heavy metal pollution has become an environmental problem that needs to be solved urgently. Repairing soil contaminated by complex heavy metals and restoring the original function of the soil are of great practical significance to the sustainable development of agriculture and the improvement of environmental quality.

[0003] At present, the methods of soil complex heavy metal remediation are mainly divided into physical remediation, physicochemical remediation, chemical remediation, biological remediation and comprehensive remediation. There are three main ways to control soil complex heavy metal pollution: one is to change the existence form of complex heavy metals in the soil, fix them, and reduce their mobility and bioavailability in the environment; the second is to remove complex heavy metals from the soil; the third is to isolate polluted areas from unpolluted areas. Physical remediation, chemical remediation, and physicochemical remediation technologies have the characteristics of strong timeliness and rapid results, but they generally have the disadvantages of high management costs and easy reactivation of complex heavy metals after remediation. Biological remediation has the advantages of low remediation cost, low ecological risk, and small side effects on the environment. It mainly includes phytoremediation, microbial remediation, and plant-microbial combined remediation.

[0004] CN105170627A discloses a method for the joint repair of cadmium-contaminated soil by microorganisms and plants, wherein ryegrass seeds are evenly sprinkled into the cadmium-containing soil, and then watering is performed to keep the soil moist. After 10-20 days, when the plant germinates and grows, a microbial composite agent is added to the ryegrass root soil, and the cadmium in the soil is removed by combining the absorption of cadmium by ryegrass and the strengthening effect of the microbial composite agent. The method improves the absorption capacity of ryegrass to cadmium and enhances the removal effect of cadmium-rich contaminated soil. However, when the cadmium content in ryegrass reaches a certain content, the growth of ryegrass will be inhibited, thereby affecting its absorption of composite heavy metals and reducing the repair effect of contaminated soil.

[0005] Chinese patent application CN114940949A discloses a method for preparing a bacterial agent for cultivating Atractylodes lancea, and a method for preparing mycorrhizal Atractylodes lancea seedlings with the prepared bacterial agent. Experiments have shown that after inoculating the bacterial agent for cultivating Atractylodes lancea of ​​the present invention, the mycorrhizal infection rate of the root system of Atractylodes lancea reaches 100%, and the biomass of Atractylodes lancea is significantly increased. The mycorrhizal dependence of the current year and the annual Atractylodes lancea reaches 36% and 52% respectively, and the dry weight of the whole plant increases by 56.2% and 108.4% respectively, both reaching a significant level. At the same time, the root length and root surface area of ​​the second-year Atractylodes lancea inoculated with the bacterial agent for cultivating Atractylodes lancea are significantly higher than those of the non-inoculated treatment.

[0006] Although currently there are technologies such as phytoremediation, in-situ passivation, bioremediation and combined remediation, many problems have not been solved in practical applications, resulting in the failure to achieve the expected remediation effect for large-scale composite heavy metal pollution. Therefore, it is necessary to provide a new solution for the remediation of large-scale composite heavy metal contaminated soil. Summary of the invention

[0007] In view of the deficiencies in the prior art, the object of the present invention is to provide a Scotch Glomerella AMG16 for repairing heavy metal contaminated soil and its application.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a Scotch Glomus caledonium AMG16 for repairing heavy metal contaminated soil. The Scotch Glomus caledonium AMG16 is classified as Glomus caledonium, has a preservation number of CGMCC NO.41628, and a preservation date of November 15, 2024.

[0010] The present invention isolates and preserves a new Scotch spherical mold AMG16 that can repair heavy metal contaminated soil from heavy metal contaminated soil. Scotch spherical mold AMG16 can help plants absorb mineral nutrients and enhance plant resistance to complex heavy metals, further change the absorption, transportation and distribution of complex heavy metals by plants, and reduce soil complex heavy metal pollution. As an indigenous strain, the strain can effectively cope with the complex soil, biological, climate and multiple adverse stress coexistence environment in the natural environment, and improve the activity of the strain after the use of the microbial agent.

[0011] In a second aspect, the present invention provides a Scotch Glomeromyces AMG16 bacterial agent for repairing heavy metal contaminated soil, wherein the Scotch Glomeromyces AMG16 bacterial agent is obtained by the following method:

[0012] The Scotch sac mold AMG16 with a preservation number of CGMCC NO.41628 is inoculated on the propagation substrate added to the root system of the host plant, and the propagation culture is carried out to obtain the Scotch sac mold AMG16 bacterial agent. Each gram of the Scotch sac mold AMG16 bacterial agent contains 80-120 (for example, 80, 85, 90, 95, 100, 105, 110, 115, 120, etc.) Scotch sac mold AMG16 spores. Other specific point values ​​within this numerical range can be selected, and they will not be repeated here.

[0013] Preferably, the multiplication medium comprises a sand-soil mixture culture medium.

[0014] Preferably, the volume ratio of river sand to soil in the sand-soil mixture culture medium is (2-4):1, for example 2:1, 2.5:1, 3:1, 3.5:1, 4:1, etc. Other specific point values ​​within this numerical range can be selected and will not be described one by one here.

[0015] Preferably, the host plant root system includes any one of corn, wheat, soybean, alfalfa, Elymus nutans, and clover, or a combination of at least two thereof.

[0016] Preferably, the time for expansion and culture is 40-60 days, for example 40 days, 42 days, 44 days, 46 days, 48 ​​days, 50 days, 52 days, 54 days, 56 days, 58 days, 60 days, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0017] In a third aspect, the present invention provides a method for repairing heavy metal contaminated soil, the method for repairing heavy metal contaminated soil comprising the following steps:

[0018] The Glomus scotti AMG16 bacterial agent for repairing heavy metal contaminated soil described in the second aspect, clay minerals and heavy metal contaminated soil are mixed, and then plants are planted.

[0019] The present invention can significantly promote plant growth by inoculating AMG16 bacterial agent in heavy metal contaminated soil, and play an important role in absorbing, transporting and fixing heavy metals. The combined use of clay minerals and AMG16 to repair heavy metal pollution can significantly increase crop biomass and reduce the heavy metal content of crop seeds. After AMG16 bacterial agent and clay minerals are applied to heavy metal contaminated soil and crops are planted, heavy metals can be fixed in the root system of crops, increasing crop yield and quality. As crops are harvested, heavy metals gradually migrate out of the soil and the soil is repaired. This soil remediation method has the advantages of low cost, simple operation, and no secondary pollution. It is suitable for heavy metal remediation of large-scale farmland soil and is of great significance for ensuring food security.

[0020] Preferably, the clay mineral includes any one of montmorillonite, kaolin, attapulgite, bentonite or sepiolite, or a combination of at least two of them.

[0021] Preferably, the clay mineral is a combination of bentonite and sepiolite.

[0022] Clay minerals are rich in reserves, low in price, have strong adsorption capacity and low risk of secondary pollution. Studies have found that minerals reduce the inhibitory effect of complex heavy metals on enzyme activity by passivating complex heavy metals, thereby enhancing enzyme activity.

[0023] Preferably, the mass ratio of bentonite to sepiolite is 1:(0.3-3), for example 1:0.3, 1:0.5, 1:0.7, 1:1, 1:1.3, 1:1.5, 1:1.7, 1:2, 1:2.3, 1:2.5, 1:2.7, 1:3, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0024] Preferably, the Scotch Glomus AMG16 inoculant, clay minerals and heavy metal contaminated soil are mixed and then plowed, with a plowing depth of 15-20 cm, for example, 15 cm, 16 cm, 17 cm, 18 cm, 19 cm, 20 cm, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0025] Preferably, the dosage of the Scotch Glomus AMG16 agent is 0.8%-5% of the soil weight at 15-20 cm in the heavy metal contaminated soil, for example 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. Other specific point values ​​within the numerical range can be selected, and they will not be described here one by one.

[0026] Preferably, the amount of the clay mineral is 0.1%-3% of the weight of the soil at 15-20 cm of the heavy metal contaminated soil, for example 0.1%, 0.3%, 0.5%, 0.7%, 0.9%, 1.1%, 1.3%, 1.5%, 1.7%, 1.9%, 2.1%, 2.3%, 2.5%, 2.7%, 3%, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0027] Preferably, the plant species include corn, wheat or soybean.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention isolates and preserves a new strain of Scotch Glomeromyces AMG16 that can repair heavy metal contaminated soil from heavy metal contaminated soil. The AMG16 strain can help plants absorb mineral nutrients and enhance plant resistance to complex heavy metals, further change the absorption, transportation and distribution of complex heavy metals by plants, and reduce soil complex heavy metal pollution. As an indigenous strain, the AMG16 strain can effectively cope with the complex soil, organisms, climate and multiple adverse stress coexisting environments in the natural environment, and improve the activity of the strain after the use of the microbial agent.

[0030] The AMG16 strain involved in the present invention is classified and named Glomus caledonium, and the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Administration. The preservation time is November 15, 2024, the preservation number is CGMCC No.41628, and the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a plate colony morphology of AMG16 in Example 1;

[0032] Figure 2 It is a phylogenetic relationship map of the AMG16 strain and related strains in Example 1. DETAILED DESCRIPTION

[0033] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0034] In the following examples, unless otherwise specified, the reagents and consumables used were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0035] The AMG16 strain involved in the following embodiment is classified and named Glomus caledonium, and the depository is the General Microbiology Center of China Microbiological Culture Collection Administration. The deposit time is November 15, 2024, the deposit number is CGMCC No.41628, and the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0036] Example 1

[0037] Isolation of Scotch Glomeromyces AMG16 strain:

[0038] (1) Screening of arbuscular mycorrhizal fungi (AMF) spores from heavy metal contaminated soils:

[0039] 1) Medium No. 1: 20 g glucose, 5 g potato extract powder, 14 g agar, pH 5.6±0.2 (25°C).

[0040] 2) Solid culture medium No. 2: glucose 10.0 g, sodium chloride 0.025 g, dipotassium hydrogen phosphate 0.5 g, malt extract powder 3.0 g, vitamin B1 0.1 mg, calcium chloride 0.05 g, diammonium hydrogen phosphate 0.25 g, ferric chloride hexahydrate 0.012 g, magnesium sulfate heptahydrate 0.15 g, agar 20 g, pH 5.7±0.1 (25°C).

[0041] 3) Take 10g of fresh heavy metal contaminated soil sample and 90mL of sterile water in a triangular flask with a sealing film (sterilized at 121℃, 0.1MPa for 30min), shake at 27℃, 150r / min, and enrich continuously for 45 days with 5 days as a cycle. After the mixed sample obtained in each cycle is allowed to stand for 30min, the upper bacterial suspension is diluted and spread by the coating method. Take 1mL of bacterial suspension and dilute it into 10 -1 Soil suspension, take 1mL10 -1 The soil suspension was obtained in test tube 2 to obtain 10 -2 Soil suspension, the same operation to obtain 10 -3 , 10 -4 , 10 -5 0.2 mL of soil sample suspension with different concentrations and 0.1 mL of 50 μg / mL streptomycin sulfate (to inhibit bacterial growth) were added to 1 solid medium and cultured in the dark at 27°C for 7 days to observe the growth of the colonies. Single colonies were selected and separated by streaking with the "Z" streak method on 2 solid medium, and cultured in the dark at 27°C for 3-4 days to obtain AMF spore groups. Single spores were separated under a stereo microscope, and the obtained single spores and the obtained bacterial groups were stored flat in a 4°C refrigerator for later use.

[0042] (2) Screening of AMG16 strain:

[0043] Accurately weigh 1.631 g of cadmium chloride, dissolve it, and filter it through a 0.22 μm filter membrane to sterilize it to obtain 10 mg mL -1 CdCl2 solution. Use a sterile pipette to accurately pipette 0, 0.2, 0.8, and 1.6 mL of CdCl2 solution into 100 mL of sterilized MMN medium, mix thoroughly, and pour onto a plate to obtain Cd concentrations of 0, 10, 20, 50, and 100 mg·L -1A series of concentration gradient MMN medium. Spore groups were selected from the activated PDA medium, and single spores were selected and transferred to the prepared MMN medium with different Cd concentrations. Each treatment was repeated 3 times and cultured at 27°C for 7 days. Spore groups that can still grow normally under high concentrations were selected under a stereo microscope, and single spore strains were isolated. It was initially selected as a Cd-resistant AMF fungal strain. The isolated Cd-resistant strain was sent to Shanghai Meiji Biological Co., Ltd. for identification and was identified as Glomus caledonium. The strain was named Scottish Glomus caledonium AMG16 strain, and the deposit number is CGMCC NO.41628.

[0044] Example 2

[0045] Identification of the Scottish Glomus AMG16 strain:

[0046] (1) Morphological observation of strains:

[0047] The spores of the AMG16 strain isolated in Example 1 were sucked up with a sterile pipette tip and transferred to a glass slide. A floating agent PVLG (consisting of lactic acid, polyvinyl alcohol and glycerol) was added near the spores. The shape, size and color of the spores, the color, layer, structure and thickness of the spore wall, the spore surface patterns and the hyphae morphology were observed and recorded under a stereomicroscope.

[0048] The spores of the AMG16 strain isolated in Example 1 have the following characteristics:

[0049] Observe and record the colony morphology of strain AMG16 on the plate. The strain has solitary spores after 5 days of culture. They are light yellow, spherical to nearly spherical, and 100μm-300μm. Spore wall: 2 layers. Layer 1 is colorless and transparent, with uniform walls, 1μm-2μm thick, slightly thickened at the connecting point, and easily separated from layer 2. Layer 2 has a spore wall, light yellow, and 2μm-8(-10)μm thick. Sporangial hyphae: mostly straight or trumpet-shaped, 9μm-12(-20)μm wide, and lighter in color. The connecting point is 10μm-35μm wide, and there is a thin septum at the connecting point (occasionally within 15μm below the connecting point). Figure 1 Shown

[0050] (2) The isolated Cd-resistant strain was sent to Shanghai Meiji Biotechnology Co., Ltd. for identification and was identified as Glomus caledonium. Its phylogenetic relationship with related strains is shown in the following table. Figure 2 shown.

[0051] Example 3

[0052] Preparation of Scottish Glomus AMG16 inoculant:

[0053] Fresh host plant roots: roots of corn, alfalfa and Elymus nutans were added to the sterilized sand-soil mixture culture medium (the volume ratio of river sand to soil was 3:1), and then the AMG16 strain was inoculated and screened in the culture medium. The inoculation amount was 350 spores per 2 kg of propagation substrate, and the propagation culture was carried out for 50 days. After the propagation, the AMF agent was obtained, and each gram of the Scotch Glomus AMG16 agent contained 120 Scotch Glomus AMG16 spores.

[0054] Example 4

[0055] Preparation of Scottish Glomus AMG16 inoculant:

[0056] Fresh host plant roots: wheat, alfalfa and Elymus nutans roots were added to the sterilized sand-soil mixture culture medium (the volume ratio of river sand to soil was 3.5:1), and then the AMG16 strain was inoculated and screened in the culture medium. The inoculation amount was 300 spores per 2 kg of propagation substrate, and the propagation culture was carried out for 40 days. After the propagation, the AMF agent was obtained, and each gram of the Scotch Glomus AMG16 agent contained 100 Scotch Glomus AMG16 spores.

[0057] Example 5

[0058] Preparation of Scottish Glomus AMG16 inoculant:

[0059] Fresh host plant roots: corn, alfalfa and clover roots were added to the sterilized sand-soil mixture culture medium (the volume ratio of river sand to soil was 2.5:1), and then the AMG16 strain was inoculated and screened in the culture medium. The inoculation amount was 300 spores per 2 kg of propagation substrate. The propagation culture was carried out for 60 days. After the propagation, the AMF agent was obtained, and each gram of the Scotch Glomus AMG16 agent contained 100 Scotch Glomus AMG16 spores.

[0060] Application Example 1

[0061] This application example provides a method for repairing heavy metal contaminated soil:

[0062] A field experiment was set up, and bentonite, sepiolite and the Scotch Glomus AMG16 inoculum obtained in Example 3 were added to the heavy metal contaminated soil, and the soil was tilled and watered to a tillage depth of 17 cm. 16 full and uniform corn seeds / m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0063] The mass ratio of bentonite to sepiolite is 1:1, and the total dosage of the two is 1% of the weight of the heavy metal contaminated soil at 15-20 cm, and the dosage of AMG16 bacterial agent is 2% of the weight of the heavy metal contaminated soil at 15-20 cm.

[0064] Application Example 2

[0065] This application example provides a method for repairing heavy metal contaminated soil:

[0066] A field experiment was set up, and bentonite, sepiolite and the Scotch Glomus AMG16 inoculum obtained in Example 4 were added to the heavy metal contaminated soil, and the soil was tilled and watered to a tillage depth of 15 cm. Full and uniformly sized corn seeds were planted in the soil and grown for 80 days.

[0067] Among them, the mass ratio of bentonite to sepiolite is 1:2, and the total dosage of the two is 0.8% of the weight of the heavy metal contaminated soil at 15-20cm, and the dosage of AMG16 bacterial agent is 2.5% of the weight of the heavy metal contaminated soil at 15-20cm.

[0068] Application Example 3

[0069] This application example provides a method for repairing heavy metal contaminated soil:

[0070] A field experiment was set up, and bentonite, sepiolite and the Scotch Glomus AMG16 inoculum obtained in Example 5 were added to the heavy metal contaminated soil, and the soil was tilled and watered to a tillage depth of 20 cm. 16 full and uniform corn seeds / m 2 Planted in soil, grown for 80 days, watered 4 times, corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0071] Among them, the mass ratio of bentonite to sepiolite is 2:1, and the total dosage of the two is 1.5% of the weight of the heavy metal contaminated soil at 15-20cm, and the dosage of AMG16 bacterial agent is 1.5% of the weight of the heavy metal contaminated soil at 15-20cm.

[0072] Application Example 4

[0073] This application example provides a method for repairing heavy metal contaminated soil:

[0074] A field experiment was set up, and sepiolite and the Scotch Glomus AMG16 inoculum obtained in Example 3 were added to the heavy metal contaminated soil, and the soil was tilled and watered at a tillage depth of 17 cm. 16 full and uniform corn seeds / m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0075] The amount of sepiolite used is 1% of the weight of the heavy metal contaminated soil at 15-20cm, and the amount of AMG16 bacterial agent used is 2% of the weight of the heavy metal contaminated soil at 15-20cm.

[0076] Application Example 5

[0077] This application example provides a method for repairing heavy metal contaminated soil:

[0078] A field experiment was set up, and bentonite and the Scotch Glomus AMG16 inoculum obtained in Example 3 were added to the heavy metal contaminated soil, and the soil was tilled and watered at a tillage depth of 17 cm. 16 full and uniform corn seeds / m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0079] The amount of bentonite used is 1% of the weight of the heavy metal contaminated soil at 15-20cm, and the amount of AMG16 bacterial agent used is 2% of the weight of the heavy metal contaminated soil at 15-20cm.

[0080] Comparative application example 1

[0081] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0082] A field experiment was set up, and the AMG16 bacterial agent of Scotch spheromone obtained in Example 3 was added to the heavy metal contaminated soil, and the soil was tilled and watered. The tillage depth was 17 cm. Full and uniform corn seeds were planted at 16 seeds / m 2 Put it into the soil, grow it for 80 days, and water it 4 times. The corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0083] The dosage of AMG16 bacterial agent is 2% of the weight of the heavy metal contaminated soil at 15-20cm.

[0084] Comparative Application Example 2

[0085] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0086] A field experiment was set up, and sepiolite was added to the heavy metal contaminated soil. The soil was tilled and watered to a tillage depth of 17 cm. 16 full and uniform corn seeds / m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0087] The amount of sepiolite used is 1% of the weight of the heavy metal contaminated soil at 15-20 cm.

[0088] Comparative Application Example 3

[0089] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0090] A field experiment was set up. Bentonite was added to the heavy metal contaminated soil, and the soil was tilled and watered to a tillage depth of 17 cm. 16 full and uniform corn seeds / m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0091] The amount of bentonite used is 1% of the weight of the heavy metal contaminated soil at 15-20 cm.

[0092] Comparative Application Example 4

[0093] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0094] A field experiment was set up. Sepiolite and bentonite were added to the heavy metal contaminated soil, and the soil was tilled and watered to a tillage depth of 17 cm. 16 full and uniform corn seeds were placed per m 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0095] The mass ratio of bentonite to sepiolite is 1:1, and the total amount of the two is 1% of the weight of the heavy metal contaminated soil at 15-20 cm.

[0096] Comparative Application Example 5

[0097] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0098] A field experiment was set up, and bentonite, sepiolite and Glomus (purchased from Glaucophyte, item number HZB413471, NBCI classification number: 1921602) were added to the heavy metal contaminated soil, and the soil was plowed and watered to a plowing depth of 17 cm. Full and uniform corn seeds were planted in the soil, grown for 80 days, and watered 4 times. The corn growth conditions were the growth conditions in Baoding, Hebei from June to August.

[0099] The mass ratio of bentonite to sepiolite is 1:1, and the total dosage of the two is 1% of the weight of the heavy metal contaminated soil at 15-20 cm, and the dosage of AMG16 bacterial agent is 2% of the weight of the heavy metal contaminated soil at 15-20 cm.

[0100] Comparative Application Example 6

[0101] This comparative application example provides a method for repairing heavy metal contaminated soil:

[0102] A field experiment was set up. No substances were added to the heavy metal-contaminated soil. 16 full and uniform-sized corn seeds were placed in the soil. 2 Planted in the soil, grown for 80 days, watered 4 times, the corn growing conditions are the same as those in Baoding, Hebei from June to August.

[0103] Test Case

[0104] This test case tests the corn and soil planted in Example 1-5 and Comparative Application Example 1-6:

[0105] Determination of soil heavy metals by microwave digestion: weigh 0.25g of air-dried soil that has passed through a 100-mesh sieve into a digestion tank, moisten it with a small amount of deionized water, add 6mL HNO3, 3mL HCl, and 2mL HF in sequence, mix well, cover and tighten after the sample and digestion solution fully react, install the digestion tank in sequence into the digestion furnace of the high-efficiency microwave digestion instrument (Yi Yao Technology, WX-7000HP), confirm that the temperature and pressure sensors are working properly, and set the digestion program according to Table 1. After the microwave digestion is completed, place the digestion tank on the acid-dripping instrument and drive the acid at 160℃ until the liquid is viscous. If there is black residue in the tube after the digestion is completed, add 2mL HNO3, 1mL HF, and 1mL HClO4 during the acid-dripping process, cover and react for 30 minutes under a slightly boiling state, then remove the cover and continue heating until the white smoke of perchloric acid disappears. Repeat the above operation until the black residue disappears. After the acid removal process was completed, the mucus was transferred to a 25 mL volumetric flask and fixed to volume. The contents of Cd and Pb in the test solution were determined using a flame atomic absorption spectrophotometer (Shimadzu, AA-7000). The results are shown in Table 2.

[0106] Table 1

[0107] step Digestion temperature Keep Time Pressure limit 1 120℃ 3min 20bar 2 160℃ 3min 35bar 3 190℃ 25min 40bar

[0108] Table 2

[0109]

[0110]

[0111] From the data in Table 2, it can be seen that the addition of clay minerals and AMG16 inoculants to heavy metal contaminated soils significantly increased the aboveground dry weight and root dry weight of corn, indicating that the combined treatment of clay minerals and AMF inoculants can promote plant growth and play an important role in the absorption, transportation and fixation of heavy metals. At the same time, the concentration of heavy metal residues in the soil increased significantly. Heavy metal residues are the most stable form of heavy metals in the soil. Under natural environmental conditions, they are not easily absorbed by plants and are not easy to migrate in water bodies, thereby reducing the risk of transmission through the food chain and contamination of groundwater.

[0112] Comparing Application Example 1 with Application Examples 4-5 and Comparative Application Examples 1-4, it can be seen that bentonite, sepiolite and AMG16 microbial agent achieve a synergistic effect in increasing crop biomass and reducing the content of heavy metals in the soil.

[0113] By comparing Application Example 1 with Comparative Application Example 5, it can be seen that the Scotch spherical mold AMG16 screened by the present invention can achieve better effects of increasing crop biomass and reducing heavy metal content in the soil compared with commercially available strains.

[0114] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0115] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0116] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A Scotch Glomerella AMG16 for repairing heavy metal contaminated soil, characterized in that: The classification name of the Scottish Glomus caledonium AMG16 is Glomus caledonium, the preservation number is CGMCC NO.41628, and the preservation time is November 15, 2024.

2. A Scotch Glomerella AMG16 bacterial agent for repairing heavy metal contaminated soil, characterized in that: The Scotch spherical mold agent is obtained by the following method: The Scotch spherical mold AMG16 with the preservation number of CGMCC NO.41628 is inoculated on the propagation substrate added to the root system of the host plant, and the Scotch spherical mold AMG16 bacterial agent is obtained, and each gram of the Scotch spherical mold AMG16 bacterial agent contains 80-120 Scotch spherical mold AMG16 spores.

3. The Glomus scotlandii AMG16 bacterial agent for repairing heavy metal contaminated soil according to claim 2, characterized in that: The propagation matrix comprises a sand-soil mixture culture medium; Preferably, the volume ratio of river sand to soil in the sand-soil mixture culture medium is (2-4):1; Preferably, the host plant root system includes any one or a combination of at least two of corn, wheat, soybean, alfalfa, Elymus nutans, and clover; Preferably, the time for expansion culture is 40-60 days.

4. A method for repairing heavy metal contaminated soil, characterized in that: The method for repairing heavy metal contaminated soil comprises the following steps: The Glomus scotti AMG16 bacterial agent described in claim 2 or 3, clay minerals and heavy metal contaminated soil are mixed, and then plants are planted.

5. The method for remediating heavy metal contaminated soil according to claim 4, characterized in that: The clay mineral includes any one of montmorillonite, kaolin, attapulgite, bentonite or sepiolite, or a combination of at least two of them.

6. The method for remediating heavy metal contaminated soil according to claim 4 or 5, characterized in that: The clay mineral is a combination of bentonite and sepiolite; Preferably, the mass ratio of bentonite to sepiolite is 1:(0.3-3).

7. The method for remediating heavy metal contaminated soil according to any one of claims 4 to 6, characterized in that: The Scottish Glomus AMG16 bacterial agent, clay minerals and heavy metal contaminated soil are mixed and then plowed to a depth of 15-20 cm.

8. The method for remediating heavy metal contaminated soil according to any one of claims 4 to 7, characterized in that: The dosage of the Scotch Glomus AMG16 bacterial agent is 0.8%-5% of the weight of the soil at 15-20cm of the heavy metal contaminated soil.

9. The method for remediating heavy metal contaminated soil according to any one of claims 4 to 8, characterized in that: The clay mineral is used in an amount of 0.1% to 3% of the weight of the soil at 15 to 20 cm of the heavy metal contaminated soil.

10. The method for remediating heavy metal contaminated soil according to any one of claims 4 to 9, characterized in that: The plant species include corn, wheat or soybean.

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