Method for domesticating aegiceras corniculatum seedlings before intertidal zone planting

Abstract

The invention discloses a domestication method for aegiceras corniculatum seedlings before intertidal zone planting. The domestication method comprises the following steps that the aegiceras corniculatum seedlings with the height not smaller than 20 cm and the number of leaves not smaller than 6 are selected; the method comprises the following steps: firstly, immersing roots of seedlings in seawater with the salinity of 1%, increasing the salinity by 0.5% every 10 days until the salinity reaches 25%, and maintaining the salinity for 10 days; immersing the tops of the aegiceras corniculatum seedlings with seawater with the salinity of 23-25% o, and submerging the aegiceras corniculatum seedlings in water for 8 hours every day for 30 days; finally, the seedlings are moved outdoors, a sunshade net is used for shielding about 50% of sunlight in the first 20 days, then the sunshade net is removed to adapt to the seedlings for 20 days, and then mud flat planting can be conducted. The survival rate of the aegiceras corniculatum seedlings domesticated by the method when the aegiceras corniculatum seedlings are planted in the mudflat land exceeds 90%, and is increased by 65% or above compared with the survival rate of the seedlings which are not domesticated, the problem that the survival rate of the aegiceras corniculatum seedlings is low when the aegiceras corniculatum seedlings are planted in the mudflat is effectively solved, and the method has important significance on increasing the success rate of mangrove forest artificial afforestation.

Description

Technical Field

[0001] The invention relates to the field of mangrove plant cultivation, in particular to a method for domesticating Paulownia truncatum seedlings before planting them on tidal flats. Background Art

[0002] Aegiceras corniculatum, also known as the candle fruit, is a shrub or small tree belonging to the genus Aegiceras in the family Myrsinaceae. This plant is mainly distributed in Hainan, Guangxi, Guangdong, Fujian, Taiwan and the South China Sea Islands in China. It grows on mudflats in the tidal zone of the sea, has strong adaptability, and easily forms a single species plant community. The tung tree is one of the indispensable tree species in the coastal mangrove ecosystem. Its community not only constitutes a unique mangrove landscape, but also plays a vital ecological function in preventing wind and waves, promoting siltation, protecting the coastline, strengthening dams, and purifying seawater and air.

[0003] Due to the impact of large-scale human activities such as reclamation of land from the sea, beach aquaculture, beach reclamation, and dock and road construction, China's mangroves have suffered serious damage in the past few decades and their area has been greatly reduced. The disappearance of mangroves has not only led to a decrease in biodiversity, but also a decline in the quality of the coastal environment and increased the risk of invasion by alien species. Therefore, artificial afforestation is needed to restore the area of ​​tung tree mangroves.

[0004] However, a major problem facing artificial afforestation of Tung trees is that the survival rate of Tung tree seedlings planted on tidal flats is low due to the harsh and complex environmental conditions of coastal tidal flats, such as high salinity, tidal erosion and flooding. In particular, indoor Tung tree seedlings are difficult to survive on wild tidal flats without artificial domestication of salinity, flooding and sunlight. The constraints of these factors have greatly hindered the restoration of Tung tree mangroves. Summary of the invention

[0005] In view of this, the present invention aims to provide a method for acclimatizing Tung tree seedlings before planting them on tidal flats, so as to ensure that the Tung tree seedlings have a higher survival rate after being planted on tidal flats.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: A method for acclimatizing Paulownia truncatum seedlings before planting on a tidal flat comprises the following steps: S1: Select Tung tree seedlings with a plant height of not less than 20 cm, not less than 6 leaves, no diseases or insect pests, healthy growth, and planted in seedling bags as the domestication objects, and place them together with the seedling bags in an indoor water tank, and perform root flooding treatment in the following order: flood the roots of the seedlings with seawater with a salinity of 10‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 15‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 20‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 25‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; S2: After step S1 is completed, the seedlings in the indoor water tank are fully flooded: the seedlings are fully submerged in seawater with a salinity of 23‰ to 25‰ prepared with sea salt, and the water surface just covers the plant growth point. The flooding time is 30 days, and the seawater is drained and replenished regularly to simulate the tide, so that the seedlings are flooded for 8 hours every day; S3: After step S2 is completed, the seedlings in the indoor water tank are moved together with the seedling bags to the outdoor water tank for root flooding treatment: the roots of the seedlings are flooded with seawater prepared with sea salt and the salinity is 23‰ to 25‰, and the water surface is below the first leaf; a sunshade net is used to block sunlight for the first 20 days, and then the sunshade net is removed to allow the seedlings to adapt to normal sunlight for 20 days.

[0007] Furthermore, the seedling raising bag adopts a non-woven textile bag, and the bag matrix is ​​composed of sea mud, sand and humus in a volume ratio of 1:1:1.

[0008] Furthermore, during the flooding treatment, evaporated water is replenished in time to ensure stable seawater salinity.

[0009] Furthermore, in step S1 and step S2, the indoor environment temperature is controlled to be 30°C and the humidity is 60%, and LED plant lamps are used to supplement the light, the lighting time is 12h / d, and the light intensity is 200μmol·m -2 ·s -1 , the light quality is R / B / G=3:1:1.

[0010] Furthermore, in step S2, the 8-hour flooding is performed in two times, each time for 4 hours, with an interval of 8 hours in between.

[0011] Furthermore, in step S3, the shading rate of the sunshade net is 50%.

[0012] The above-mentioned method for acclimatizing Tung tree seedlings before planting on tidal flats is applied in improving the survival rate of Tung tree seedlings on tidal flats.

[0013] The above-mentioned method for acclimatizing tung tree seedlings before planting on tidal flats is used to promote the growth of tung trees on tidal flats. DETAILED DESCRIPTION

[0014] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] Embodiment 1: A method for taming Tung tree seedlings before planting on tidal flats is carried out according to the following steps: S1: Select Paulownia truncatum seedlings with a plant height of not less than 20 cm, not less than 6 leaves, no diseases and insect pests, healthy growth, and planted in seedling bags as the domestication objects, place them together with the seedling bags in an indoor water tank, and perform root flooding treatment in the following order: flood the roots of the seedlings with seawater with a salinity of 10‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 15‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 20‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 25‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days. The seedling bags were 83cm×11.8cm non-woven textile bags, and the bag matrix consisted of sea mud, sand and humus in a volume ratio of 1:1:1. During the flooding treatment, the evaporated water was replenished in time to ensure the stability of seawater salinity. At the same time, the indoor environment temperature was controlled at 30℃ and the humidity was 60%. LED plant lights were used to supplement the light, with a lighting time of 12h / d and a light intensity of 200μmol·m -2 ·s -1 , the light quality is R / B / G=3:1:1.

[0016] S2: After step S1 is completed, the seedlings in the indoor water tank are fully flooded: the whole seedling is submerged in seawater with a salinity of 23‰ to 25‰ prepared with sea salt, and the water surface just covers the plant growth point. The treatment time is 30 days, and the seawater is drained and replenished regularly to simulate the tide, so that the seedlings are flooded for 8 hours a day (8 hours of flooding is carried out twice, 4 hours each time, and there is an interval of 8 hours in between). During the flooding treatment, the evaporated water is replenished in time to ensure the stability of the seawater salinity; at the same time, the indoor environment temperature is controlled at 30℃ and the humidity is 60%, and LED plant lights are used to supplement the light. The lighting time is 12h / d and the light intensity is 200μmol·m -2 ·s -1, the light quality is R / B / G=3:1:1.

[0017] S3: After step S2 is completed, the seedlings in the indoor water tank are moved together with the seedling bags to the outdoor water tank for root flooding treatment: the roots of the seedlings are flooded with seawater with a salinity of 23‰ to 25‰ prepared with sea salt, and the water surface is below the first leaf. During the flooding treatment, the evaporated water is replenished in time to ensure the stability of the seawater salinity; at the same time, a sunshade net with a 50% shading rate is used to block sunlight for the first 20 days, and then the sunshade net is removed to allow the seedlings to adapt to normal sunlight for 20 days before being ready to be planted on the tidal flat land.

[0018] Embodiment 2: A method for taming Tung tree seedlings before planting on tidal flats is carried out according to the following steps: S1: Select Paulownia truncatum seedlings with a plant height of not less than 20 cm, not less than 6 leaves, no diseases and insect pests, healthy growth, and planted in seedling bags as the domestication objects, place them together with the seedling bags in an indoor water tank, and perform root flooding treatment in the following order: flood the roots of the seedlings with seawater with a salinity of 10‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 15‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 20‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 25‰ prepared by sea salt, the water surface is below the first leaf, and the flooding time is 10 days. The seedling bags were 83cm×11.8cm non-woven textile bags, and the bag matrix consisted of sea mud, sand and humus in a volume ratio of 1:1:1. During the flooding treatment, the evaporated water was replenished in time to ensure the stability of seawater salinity. At the same time, the indoor environment temperature was controlled at 30℃ and the humidity was 60%. LED plant lights were used to supplement the light, with a lighting time of 12h / d and a light intensity of 200μmol·m -2 ·s -1 , the light quality is R / B / G=3:1:1.

[0019] S2: After step S1 is completed, the seedlings in the indoor water tank are fully flooded: the whole seedling is submerged in seawater with a salinity of 23‰ to 25‰ prepared with sea salt, and the water surface just covers the plant growth point. After 30 days of treatment, it is ready to be planted on the tidal flat land. The seawater is drained and replenished regularly to simulate the tide, so that the seedlings are flooded for 8 hours a day (8 hours of flooding is carried out twice, 4 hours each time, and there is an interval of 8 hours in between). During the flooding treatment, the evaporated water is replenished in time to ensure the stability of the seawater salinity; at the same time, the indoor environment temperature is controlled at 30℃ and the humidity is 60%, and LED plant lights are used to supplement the light. The lighting time is 12h / d and the light intensity is 200μmol·m -2 ·s -1, the light quality is R / B / G=3:1:1.

[0020] Embodiment 3: A method for taming Tung tree seedlings before planting on tidal flats is carried out according to the following steps: Tung tree seedlings with a plant height of not less than 20 cm, not less than 6 leaves, no diseases and insect pests, healthy growth, and planted in seedling bags were selected as the domestication objects, and placed in an indoor water tank together with the seedling bags, and the root flooding treatment was carried out in the following order: the roots of the seedlings were flooded with seawater with a salinity of 10‰ prepared by sea salt, the water surface was below the first leaf, and the flooding time was 10 days; then the roots of the seedlings were flooded with seawater with a salinity of 15‰ prepared by sea salt, the water surface was below the first leaf, and the flooding time was 10 days; then the roots of the seedlings were flooded with seawater with a salinity of 20‰ prepared by sea salt, the water surface was below the first leaf, and the flooding time was 10 days; then the roots of the seedlings were flooded with seawater with a salinity of 25‰ prepared by sea salt, the water surface was below the first leaf, and the flooding time was 10 days. The seedling bags were 83cm×11.8cm non-woven textile bags, and the bag matrix consisted of sea mud, sand and humus in a volume ratio of 1:1:1. During the flooding treatment, the evaporated water was replenished in time to ensure the stability of seawater salinity. At the same time, the indoor environment temperature was controlled at 30℃ and the humidity was 60%. LED plant lights were used to supplement the light, with a lighting time of 12h / d and a light intensity of 200μmol·m -2 ·s -1 , the light quality is R / B / G = 3:1:1. After the flooding treatment is completed, the land is ready for planting on the tidal flat.

[0021] Ensure that Examples 1 to 3 are domesticated on the same day, and select the same batch of indoor undomesticated Paulownia tree seedlings (control) with similar growth to be planted together with the domesticated seedlings of Examples 1 to 3 in the tidal flat land high tide area with a seawater salinity of 25‰-30‰, and compare the growth and survival rate after 90 days.

[0022] As can be seen from Table 1, the plant height growth, leaf number growth and survival rate of Example 1 are the highest, followed by Example 2, then Example 3, and the worst is the undomesticated Paulownia truncatum seedlings. The survival rate of the Paulownia truncatum seedlings of Example 1 reached 91.7%, while the survival rate of the undomesticated Paulownia truncatum seedlings was only 25%.

[0023] Table 1 Growth and survival rate of Tung tree seedlings The above describes the specific content of the present invention through embodiments. For those skilled in the art, it is easy to make several changes and modifications to the content of the present invention based on known principles, so the above embodiments do not represent the entire patent protection scope of the present invention. Therefore, in accordance with the concept limited by the claims, modifications based on the present invention also belong to the protection scope of the claims.

Claims

1. A method for acclimatizing Tung tree seedlings before planting on tidal flats, characterized in that: The following steps are involved: S1: Select Tung tree seedlings with a plant height of not less than 20 cm, not less than 6 leaves, no diseases or insect pests, healthy growth, and planted in seedling bags as the domestication objects, and place them together with the seedling bags in an indoor water tank, and perform root flooding treatment in the following order: flood the roots of the seedlings with seawater with a salinity of 10‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 15‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 20‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; then flood the roots of the seedlings with seawater with a salinity of 25‰ prepared by sea salt, with the water surface below the first leaf, and the flooding time is 10 days; S2: After step S1 is completed, the seedlings in the indoor water tank are fully flooded: the seedlings are fully submerged in seawater with a salinity of 23‰~25‰ prepared with sea salt, and the water surface just covers the plant growth point. The treatment time is 30 days, and the seawater is drained and replenished regularly to simulate the tide, so that the seedlings are flooded for 8 hours every day; S3: After step S2 is completed, the seedlings in the indoor water tank together with the seedling bags are moved to the outdoor water tank for root flooding treatment: the roots of the seedlings are flooded with seawater prepared with sea salt and the salinity is 23‰~25‰, and the water surface is below the first leaf; the sunshade net is used to block the sunlight for the first 20 days, and then the sunshade net is removed to allow the seedlings to adapt to normal sunlight for 20 days.

2. The acclimation method according to claim 1, characterized in that: The seedling bag is made of non-woven textile bag, and the bag matrix is ​​composed of sea mud, sand and humus in a volume ratio of 1:1:

1.

3. The acclimation method according to claim 1, characterized in that: During the flooding treatment, evaporated water is replenished in time to ensure stable seawater salinity.

4. The acclimation method according to claim 1, characterized in that: In step S1 and step S2, the indoor environment temperature is controlled to be 30°C and the humidity is 60%. LED plant lamps are used to supplement the light. The lighting time is 12h / d and the light intensity is 200μmol·m -2 ·s -1 , the light quality is R / B / G=3:1:

1.

5. The acclimation method according to claim 1, characterized in that: In step S2, the 8-hour flooding is performed in two times, each time for 4 hours, with an interval of 8 hours in between.

6. The acclimation method according to claim 1, characterized in that: In step S3, the shading rate of the sunshade net is 50%.

7. Use of the method according to any one of claims 1 to 5 in improving the survival rate of Tung trees on tidal flats.

8. Use of the method according to any one of claims 1 to 5 in promoting the growth of Tung trees on tidal flats.

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