A method for acclimatizing a jatropha curcas seedling before planting on a beach

Abstract

The application discloses a domestication method for Aegiceras corniculatum seedlings before planting on a beach, and comprises the following steps: selecting Aegiceras corniculatum seedlings with a height of not less than 20 cm and a leaf number of not less than 6; firstly, submerging the seedling roots in seawater with a salinity of 10‰, increasing the salinity by 5‰ every 10 days until the salinity reaches 25‰, and maintaining the salinity for 10 days; then, submerging the Aegicericas corniculatum seedlings in seawater with a salinity of 23-25‰ to the top of the seedlings, submerging the seedlings in water for 8 hours every day, and continuing for 30 days; finally, moving the seedlings outdoors, shielding about 50% sunlight of the seedlings by using a sunshade net for 20 days, then removing the sunshade net and adapting the seedlings for another 20 days, and then planting the seedlings on the beach. The survival rate of the Aegicericas corniculatum seedlings domesticated by the method is more than 90% when planted on the beach, the survival rate is increased by more than 65% compared with the seedlings without domestication, the problem of low survival rate of the Aegicericas corniculatum seedlings when planted on the beach is effectively solved, and the method has important significance for improving the success rate of mangrove artificial afforestation.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of mangrove cultivation, in particular to a domestication method of Avicennia marina seedlings before planting on mudflat. BACKGROUND

[0002] Avicennia marina (Forst.f.) Vahl Aegiceras corniculatum ), also known as candlefruit, is a shrub or small tree belonging to the Myrsinaceae family. It grows on mudflats in the tidal zone of the sea and has strong adaptability and can easily form a single-species plant community. Avicennia marina is one of the indispensable tree species in the coastal mangrove ecosystem. Its community not only constitutes a unique mangrove landscape, but also plays a crucial ecological function in wind protection, wave dissipation, promotion of sediment deposition, protection of coastlines, reinforcement of dikes, and purification of seawater and air.

[0003] The disappearance of mangroves not only leads to a decrease in biodiversity, but also causes a decline in the quality of the coastal environment and increases the risk of invasion by alien species. Therefore, artificial afforestation is needed to restore the area of Avicennia marina mangrove.

[0004] However, one of the major problems facing Avicennia marina artificial afforestation is that the coastal mudflat environment is harsh and complex, such as high salinity, tidal flushing and submergence, which results in low survival rate of Avicennia marina seedlings planted on mudflat land. In particular, Avicennia marina seedlings cultivated indoors are difficult to survive on the wild mudflat land without artificial domestication of salinity, water submergence and sunlight. These factors greatly hinder the restoration of Avicennia marina mangrove. SUMMARY

[0005] In view of this, the present application aims to provide a domestication method of Avicennia marina seedlings before planting on mudflat, to ensure that the Avicennia marina seedlings have a high survival rate after planting on mudflat land.

[0006] In order to achieve the above-mentioned application purpose, the present application provides the following technical solutions:

[0007] A domestication method of Avicennia marina seedlings before planting on mudflat, comprising the following steps:

[0008] S1: Selecting the tung tree seedlings with a plant height not less than 20 cm, a leaf number not less than 6, no disease and insect pests, healthy growth and planted in the seedling bag as the domestication object, and placing the seedling bag as a whole in the indoor water tank, and then performing root flooding treatment in the following order: submerging the roots of the seedling in seawater with a salinity of 10 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; submerging the roots of the seedling in seawater with a salinity of 15 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; submerging the roots of the seedling in seawater with a salinity of 20 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; and submerging the roots of the seedling in seawater with a salinity of 25 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days;

[0009] S2: After step S1 is completed, performing whole flooding treatment on the seedling in the indoor water tank: submerging the whole seedling in seawater with a salinity of 23 ‰ to 25 ‰ prepared by sea salt, with the water surface just submerging the growth point of the plant, for 30 days, and regularly emptying and replenishing seawater to simulate tides, so that the seedling is submerged in seawater for 8 hours per day;

[0010] S3: After step S2 is completed, moving the seedling in the indoor water tank as a whole to the outdoor water tank, and performing root flooding treatment: submerging the roots of the seedling in seawater with a salinity of 23 ‰ to 25 ‰ prepared by sea salt, with the water surface below the first leaf; using a sunshade net to shield sunlight for the first 20 days, and then removing the sunshade net to allow the seedling to adapt to normal sunlight for 20 days.

[0011] Further, the seedling bag is a non-woven fabric textile bag, and the bagged substrate is composed of sea mud, sand and humus soil in a volume ratio of 1:1:1.

[0012] Further, the evaporated water is replenished in time during the flooding treatment, so as to ensure the stability of the salinity of the seawater.

[0013] Further, in steps S1 and S2, the indoor environment temperature is controlled to be 30 ℃, the humidity is controlled to be 60%, LED plant lamps are used to supplement light, the light time is 12 h / d, the light intensity is 200 μmol·m -2 ·s -1 , and the light quality is R / B / G=3:1:1.

[0014] Further, in step S2, the 8h water submerging is performed twice, each for 4h, and the interval is 8h.

[0015] Further, in step S3, the shading rate of the sunshade net is 50%.

[0016] The above tung tree seedling domestication method before planting on the beach is applied to improve the survival rate of the tung tree on the beach.

[0017] The application of the domestication method of the above-mentioned tung tree seedlings before planting on a beach to promote the growth of tung trees on beach land. DETAILED DESCRIPTION

[0018] The technical solutions of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0019] Embodiment 1

[0020] A domestication method of tung tree seedlings before planting on a beach is carried out according to the following steps:

[0021] S1: Selecting tung tree seedlings with a plant height not less than 20 cm, a leaf number not less than 6, no disease and insect pests, healthy growth, and planted in a seedling bag as the domestication object, and placing the seedling bag as a whole in an indoor water tank, and carrying out root flooding treatment according to the following sequence: submerging the roots of the seedlings in seawater with a salinity of 10 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerging the roots of the seedlings in seawater with a salinity of 15 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerging the roots of the seedlings in seawater with a salinity of 20 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; and then submerging the roots of the seedlings in seawater with a salinity of 25 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days. The seedling bag adopts a non-woven fabric textile bag with a size of 83 cm x 11.8 cm, and the bagged substrate is composed of sea mud, sand and humus soil in a volume ratio of 1:1:1; the evaporated water is replenished in time during the flooding treatment to ensure the stability of the salinity of the seawater; at the same time, the indoor environmental temperature is controlled at 30 ℃, the humidity is controlled at 60%, LED plant lamps are used to supplement the light, the light time is 12 h / d, the light intensity is 200 μmol·m-2s-1, and the light quality is R / B / G=3:1:1. -2 -1

[0022] S2: After step S1 is completed, the seedlings in the indoor water tank are subjected to whole flooding treatment: submerging the whole seedlings in seawater with a salinity of 23 ‰-25 ‰ prepared by sea salt, with the water surface just submerging the growth point of the plant, for 30 days, and emptying and replenishing the seawater at regular time intervals to simulate tides, so that the seedlings are subjected to flooding for 8 hours every day (8 hours of flooding is divided into two times, each time for 4 hours, and the interval between the two times is 8 hours). The evaporated water is replenished in time during the flooding treatment to ensure the stability of the salinity of the seawater; at the same time, the indoor environmental temperature is controlled at 30 ℃, the humidity is controlled at 60%, LED plant lamps are used to supplement the light, the light time is 12 h / d, the light intensity is 200 μmol·m-2s-1, and the light quality is R / B / G=3:1:1.​​-2 ·s -1 , the light quality is R / B / G=3:1:1.

[0023] S3: After step S2 is completed, the seedlings in the indoor water tank are moved to the outdoor water tank together with the seedling bags for root flooding treatment: the roots of the seedlings are submerged with seawater with a salinity of 23‰-25‰ prepared by sea salt, and the water surface is below the first leaf. Evaporated water is replenished in time during the flooding treatment to ensure stable seawater salinity; at the same time, a shading net with a shading rate of 50% is used to shade sunlight for the first 20 days, and then the shading net is removed to allow the seedlings to adapt to normal sunlight for 20 days before planting on the beach land.

[0024] Example 2:

[0025] A method for acclimatizing Aegiceras corniculatum seedlings before planting on the beach, which is carried out according to the following steps:

[0026] S1: Select Aegiceras corniculatum seedlings with a plant height of not less than 20 cm, a leaf number of not less than 6, no pests and diseases, healthy growth, and planted in seedling bags as acclimatization objects, and place them together with the seedling bags in an indoor water tank for root flooding treatment in the following order: submerge the roots of the seedlings with seawater with a salinity of 10‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerge the roots of the seedlings with seawater with a salinity of 15‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerge the roots of the seedlings with seawater with a salinity of 20‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerge the roots of the seedlings with seawater with a salinity of 25‰ prepared by sea salt, with the water surface below the first leaf, for 10 days. The seedling bags are 83 cm x 11.8 cm non-woven fabric textile bags, and the bagged substrate is composed of sea mud, sand and humus soil in a volume ratio of 1:1:1; evaporated water is replenished in time during the flooding treatment to ensure stable seawater salinity; at the same time, the indoor environmental temperature is controlled at 30°C and the humidity is controlled at 60%, LED plant lamps are used to supplement light, the light time is 12h / d, and the light intensity is 200μmol·m -2 ·s -1 , the light quality is R / B / G=3:1:1.

[0027] S2: After step S1 is completed, the seedlings in the indoor water tank are subjected to full flooding treatment: the whole seedlings are submerged in seawater with a salinity of 23‰-25‰ prepared by sea salt, the water surface just submerges the growth point of the plants, and after 30 days of treatment, the seedlings are ready to be planted on the tidal flat land. The seawater is emptied and replenished at regular intervals to simulate tides, so that the seedlings are submerged in seawater for 8 hours every day (8 hours of submersion is divided into two times, 4 hours each time, and an interval of 8 hours in between). The evaporated water is replenished in time during the submersion treatment to ensure the stability of the salinity of the seawater; at the same time, the indoor environmental temperature is controlled at 30℃, the humidity is controlled at 60%, LED plant lamps are used to supplement light, the light time is 12h / d, the light intensity is 200μmol·m -2 ·s -1 , and the light quality is R / B / G=3:1:1.

[0028] Example 3:

[0029] A method for acclimatizing Aegiceras corniculatum seedlings before planting on a tidal flat, which is carried out according to the following steps:

[0030] Select Aegiceras corniculatum seedlings with a plant height not less than 20 cm, a leaf number not less than 6, no pests and diseases, healthy growth, and planted in a seedling bag as the acclimatization object, and place the seedling bag as a whole in an indoor water tank. The root submersion treatment is carried out in the following order: submerge the seedling roots in seawater with a salinity of 10‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerge the seedling roots in seawater with a salinity of 15‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; then submerge the seedling roots in seawater with a salinity of 20‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; and then submerge the seedling roots in seawater with a salinity of 25‰ prepared by sea salt, with the water surface below the first leaf, for 10 days. The seedling bag is a non-woven fabric textile bag with a size of 83cm×11.8cm, and the bagged substrate is composed of sea mud, sand and humus soil in a volume ratio of 1:1:1; the evaporated water is replenished in time during the submersion treatment to ensure the stability of the salinity of the seawater; at the same time, the indoor environmental temperature is controlled at 30℃, the humidity is controlled at 60%, LED plant lamps are used to supplement light, the light time is 12h / d, the light intensity is 200μmol·m -2 ·s -1 , and the light quality is R / B / G=3:1:1. After the submersion treatment is completed, the seedlings are ready to be planted on the tidal flat land.

[0031] Ensure that Examples 1-3 are completed on the same day, and select the same batch of indoor cultivated Aegiceras corniculatum seedlings that have not been acclimatized and have similar growth (control) to be planted together with the acclimatized seedlings of Examples 1-3 in the high tide area of the tidal flat land with a seawater salinity of 25‰-30‰, and compare the growth and survival rates after 90 days.

[0032] As can be seen from Table 1, the height growth, leaf number growth and survival rate of the A. integrifolia seedlings of Example 1 are the highest, followed by Example 2, then Example 3, and the worst is the A. integrifolia seedlings without domestication. The survival rate of the A. integrifolia seedlings of Example 1 reaches 91.7%, while the survival rate of the A. integrifolia seedlings without domestication is only 25%.

[0033] Table 1 Growth and survival rate of A. integrifolia seedlings

[0034]

[0035] The above describes the specific content of the present application through examples, and for those skilled in the art, based on known principles, it is easy to make several changes and modifications to the content of the present application, so the above examples do not represent the whole patent protection range of the present application. Therefore, the modification based on the present application within the concept limited by the claims also belongs to the protection range of the claims.

Claims

1. A method for acclimatizing a Melia azedarach seedling before planting on a beach, characterized by: Comprising the following steps: S1: Selecting seedlings of tung tree with a plant height of no less than 20 cm, a leaf number of no less than 6, no disease and insect pests, healthy growth, and planted in a seedling bag as domestication objects, and placing the seedling bag as a whole in an indoor water tank, and performing root flooding treatment in the following order: submerging the roots of the seedlings in seawater with a salinity of 10 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; submerging the roots of the seedlings in seawater with a salinity of 15 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; submerging the roots of the seedlings in seawater with a salinity of 20 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; and submerging the roots of the seedlings in seawater with a salinity of 25 ‰ prepared by sea salt, with the water surface below the first leaf, for 10 days; S2: After step S1 is completed, performing full flooding treatment on the seedlings in the indoor water tank: submerging the whole seedlings in seawater with a salinity of 23 ‰ to 25 ‰ prepared by sea salt, with the water surface just submerging the growth point of the plants, for 30 days, and regularly emptying and replenishing seawater to simulate tides, so that the seedlings are submerged in seawater for 8 hours per day; S3: After step S2 is completed, moving the seedlings in the indoor water tank as a whole together with the seedling bag to an outdoor water tank, and performing root flooding treatment: submerging the roots of the seedlings in seawater with a salinity of 23 ‰ to 25 ‰ prepared by sea salt, with the water surface below the first leaf; using a sunshade net to shield sunlight for the first 20 days, and then removing the sunshade net to allow the seedlings to adapt to normal sunlight for 20 days.

2. The domestication method of claim 1, wherein: The seedling bag is a non-woven fabric textile bag, and the bagged substrate is composed of sea mud, sand and humus soil in a volume ratio of 1:1:

1.

3. The domestication method of claim 1, wherein: The water evaporated during the flooding treatment is replenished in time to ensure the stability of the salinity of the seawater.

4. The domestication method of claim 1, wherein: In step S1 and step S2, the indoor environment temperature is controlled to be 30℃, the humidity is 60%, LED plant light is used to supplement light, the light time is 12h / d, the light intensity is 200μmol·m -2 ·s -1 -2, and the light quality is R / B / G=3:1:

1.

5. The domestication method of claim 1, wherein: In step S2, the 8-hour water submersion is performed in two times, each for 4 hours, with an interval of 8 hours.

6. The domestication method of claim 1, wherein: In step S3, the shading rate of the sunshade net is 50%.

7. Use of the method of any one of claims 1 to 5 in improving the survival rate of tung tree on tidal flat land.

8. Use of the method of any one of claims 1 to 5 in promoting the growth of tung tree on tidal flat land.

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