Artificial greenhouse planting method for pogonatum angustifolium

Abstract

The invention discloses an artificial greenhouse planting method for pogonatum angustifolium. The artificial greenhouse planting method comprises the following steps: (1) putting collected pogonatum angustifolium into a knob's culture medium; (2) adding a mixed solution containing abscisic acid with the concentration of 0.7 to 1.0 mg / L, ascorbic acid with the concentration of 0.3 to 0.5 mg / L and ergothioneine with the concentration of 1.7 to 2.3 mg / L into the culture; (3) cutting off newly-grown stress-resistant plants, performing detoxification treatment by using a bronopol aqueous solution, and performing ultrasonic disinfection to obtain detoxified plants; according to the method, the production efficiency can be remarkably improved, large-scale planting of pogonatum aloides is achieved, and sales products are obtained through direct planting.

Description

Technical Field

[0001] The present application relates to the technical field of white-hair moss cultivation, and in particular to a method for cultivating white-hair moss in an artificial greenhouse. Background Art

[0002] Leucobryum bowringii Mitt. of the Leucobryaceae family has attracted widespread attention due to its unique appearance and application value. At present, there are about 80 species of Leucobryum in the world, and four species are mainly distributed in China, including Leucobryum bowringii, Leucobryum juniperoideum Müll.Hal., Leucobryum boninense Sull.&Lesq., and Leucobryum javense (Brid.) Mitt.

[0003] Among them, the narrow-leaved white hair moss has significant advantages in bonsai art and grass tank landscaping due to its dense and compact structure and unique color contrast. It is widely used as a landscaping material in various gardens and potted landscapes.

[0004] Although wild white-hair moss resources are relatively abundant in the woodlands of the south, the current training process is to collect local wild white-hair moss, domesticate it, and adapt it to the indoor environment; however, large-scale artificial breeding has not yet been achieved.

[0005] At present, the technology in this field is still not mature enough, and most of them are small-scale cultivations carried out by moss enthusiasts for landscape landscaping, and large-scale production cannot be achieved. For example, CN202210369820.2 discloses a method for cultivating white-haired moss, which uses wild cypress leaf white-haired moss as raw material to directly cultivate white-haired moss on pine bark stone; this method can obtain landscaping materials integrated with pine bark stone without transplanting. However, this method does not explain how to achieve large-scale cultivation of white-haired moss, and this method is not suitable for large-scale production of white-haired moss.

[0006] Usually, they are laid directly on bonsai or slightly acidic substrates (such as peat soil, vermiculite), which help to reproduce their natural ecological conditions and facilitate their growth.

[0007] However, the cultivation and breeding of white-haired moss faces multiple challenges. On the one hand, the plant has high requirements for the temperature, humidity and light of the growth environment. The artificial cultivation process needs to ensure accurate control of various environmental parameters. However, during large-scale cultivation, due to the large area and high floor height of the greenhouse, in order to increase the efficiency of the greenhouse area, it is difficult to ensure that the cultivation microenvironment at different heights in the greenhouse is accurately controlled to meet the various growth requirements of the species. This makes large-scale cultivation of white-haired moss more difficult.

[0008] On the other hand, white hair moss grows slowly and is highly sensitive to environmental changes and pollutants. Existing cultivation methods make it difficult to maintain a good environment during its growth cycle. The above factors limit the reproduction and industrialization speed of white hair moss.

[0009] In addition, due to the high cost of facility cultivation, the requirements for environmental monitoring and regulation increase operating costs, resulting in high costs for white hair moss cultivation. At the same time, China lacks relatively mature white hair moss cultivation technology and expertise.

[0010] Therefore, there is an urgent need to develop and optimize new technologies and methods suitable for large-scale cultivation and management of white-hair moss to meet its market demand in bonsai gardening and landscape decoration.

[0011] For example, CN202311186009.1 discloses a method for promoting the increase of growth points of white hair moss leaves, a method for rapid regeneration and an application thereof. The method uses a white hair moss detached leaf to be placed on brassinolide impregnated paper, 24°C, a light intensity of 60-80lx, a photoperiod of 16h light and 8h dark, a humidity of 45.3%, and a 5mL brassinolide solution is regularly supplemented every five days. After 12 days of cultivation, the number of attachment points per millimeter of detached leaf is 1.1±0.07; the proportion of new attachment points is 1.00±0.00. Relative to the comparative example, the proportion and number of new attachment points are significantly increased, which is conducive to accelerating the growth rate of white hair moss. However, this method only realizes the setting of white hair moss on brassinolide moistened paper, realizes the germination of white hair moss detached leaf, and does not perform subsequent transplanting and colonization operations, and cannot obtain white hair moss that can be directly used for sale.

[0012] Based on this, innovating and researching the cultivation methods of white hair moss and exploring more adaptable and cost-effective cultivation technologies are the key paths to promote its commercial application. This is also the core problem that this patent is committed to solving.

[0013] The information disclosed in the background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0014] In view of the above technical problems, the present application provides a method for artificial greenhouse cultivation of narrow-leaved white-hair moss, which can significantly improve production efficiency, realize large-scale cultivation of white-hair moss, and directly cultivate and obtain sales products.

[0015] The present application provides a method for growing Hoary Hairy Moss in an artificial greenhouse, comprising the following steps:

[0016] (1) The collected Homotrichum angustifolia was placed in Knop's medium under a constant light intensity of 38-42 μmol·m -2 ·s-1 , culturing in an incubator at a relative humidity of 75-80% and a temperature of 20-22° C. until new shoots grow, thereby obtaining a culture;

[0017] (2) After adding a mixed solution containing 0.7-1.0 mg / L of abscisic acid, 0.3-0.5 mg / L of ascorbic acid, and 1.7-2.3 mg / L of ergothioneine to the culture, high temperature stress screening culture was carried out for 3 months. During the high temperature stress screening culture, the average daily watering amount was 170-220 mL / m 2 ·d. Apply nutrient solution to the leaves every 7 days, spray 35-45 mL of the first nutrient solution per square decimeter. After the high temperature stress screening and cultivation is completed, the surviving plants are selected as stress-resistant plants;

[0018] (3) cutting off the newly emerged stress-resistant strains, detoxifying them with a 20% bronopol aqueous solution, and then ultrasonically sterilizing them to obtain the detoxified strains;

[0019] (4) Prepare substrate soil and spread it on moss cloth, which is laid on a floating foam board and floated on the surface of a pool in a greenhouse. The virus-free strains are scattered on the surface of the substrate soil at intervals of 0.3 to 0.7 cm in the upper, lower, left and right directions, and watered thoroughly. The ambient temperature in the greenhouse is in the range of 15 to 30° C., the relative humidity is in the range of 50 to 90%, and the light intensity under the sunshade net is <100 μmol·m-2·s-1. The white-haired moss is propagated until the white-haired moss continues to proliferate and grow, and the intervals between the ramets on the moss cloth disappear and become one piece, completing the first cycle of cultivation;

[0020] During the propagation period of white hair moss: the average daily watering amount is 200mL / (m 2 ·d) Spray the second nutrient solution once every 7 days, with the spraying amount of the second nutrient solution being 35-45 mL per square decimeter. During this period, spray Bacillus subtilis solution once in the evening on the 15th day of each month, and spray carbendazim once at the end of each month. Ventilate the greenhouse 2-3 times at night every week;

[0021] The matrix soil used is obtained by mixing peat soil, vermiculite, diatomaceous earth and α-calcium ketoglutarate in a mass ratio of 1.5-2.5:1:1:1.

[0022] Preferably, the high temperature stress screening culture comprises:

[0023] In the first month, the light intensity was 78-82 μmol·m -2 ·s -1 , the temperature is 29-32°C, the relative humidity is 78-82%, and the high temperature stress culture is carried out for 30-31 days;

[0024] In the second month, the light intensity was kept constant at 180-220 μmol·m -2 ·s-1 , high light and high temperature stress culture was carried out at a temperature of 28-32°C and a relative humidity of 78-82% for 30-31 days;

[0025] In the third month, the light intensity was kept constant at 180-220 μmol·m -2 ·s -1 The culture was carried out under high light, high temperature and low humidity stress at a temperature of 29-32°C and a relative humidity of 58-62%, and the stress treatment lasted for 30-31 days.

[0026] Preferably, the second nutrient solution is composed of: Knop's nutrient solution diluted 8 times, to which 2mmol·L -1 α-Ketoglutaric acid, 10mgL -1 Vitamin C, 10mgL -1 GA3, 2mgL -1 NAA, 5mgL-1 brassinolide.

[0027] Preferably, the organic matter content of the peat soil used is less than 40%.

[0028] Preferably, the first nutrient solution used is composed of: a 10-fold diluted Knop's nutrient solution, to which 2 mmol·L -1 α-peptidase, 10mgL -1 PQQ, 2mgL -1 NAA, 0.1% concentration of chlorophyll foliar fertilizer.

[0029] Preferably, Knop's medium is potassium nitrate (KNO3): 1.0 g / L, potassium dihydrogen phosphate (KH2PO4): 0.25 g / L, magnesium sulfate (MgSO4·7H2O): 0.25 g / L, calcium chloride (CaCl2·2H2O): 0.12 g / L, boric acid (H3BO3): 0.014 g / L, manganese chloride (MnCl2·4H2O): 0.0036 g / L, zinc sulfate (ZnSO4·7H2O): 0.0028 g / L, ferrous sulfate (FeSO4·7H2O): 0.0028 g / L, copper sulfate (CuSO4·5H2O): 0.0003 g / L, ammonium heptamolybdate ((NH4)6Mo7O 24 ·4H2O): 0.0003g / L. Gelatin or agar: 10g / L, adjust pH to 5 with HCl, sterilize and heat-inject into a culture dish to obtain the culture medium.

[0030] Preferably, the Bacillus subtilis suspension is diluted 800 to 1100 times to obtain a Bacillus subtilis solution.

[0031] Preferably, the first cycle cultivation time is 4 months.

[0032] The beneficial effects of this application include:

[0033] 1) The artificial greenhouse cultivation method of narrow-leaved white hair moss provided in the present application can increase the growth rate of white hair moss, reduce the adverse effects of the planting environment on the growth of white hair moss, and also improve the stress resistance and adaptability of white hair moss during the planting process.

[0034] 2) The artificial greenhouse cultivation method of narrow-leaved white-haired moss provided in the present application not only shortens the breeding period of white-haired moss, but also improves the adaptability of the plant in the large-scale cultivation environment in the artificial greenhouse, reduces the disease rate of white-haired moss during large-scale cultivation, and can also improve the moss's absorption efficiency of various nutrients, effectively increase the planting yield, and at the same time effectively increase the growth rate of white-haired moss, accelerate the moss growth rate and biomass accumulation, and can effectively maintain the stability of the color and morphological traits of the mother plant. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 A real photo of the plants grown in the field obtained by the artificial greenhouse planting method of the narrow-leaved white hair moss in Example 1 provided in this application;

[0036] Figure 2 The actual photo of the narrow-leaved Holothrix plant obtained by the method in Example 1 provided in the present application; as can be seen from the figure, the height of a single narrow-leaved Holothrix plant obtained by planting can reach about 1.3 cm. DETAILED DESCRIPTION

[0037] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments, but the present invention is not limited in any way. Any changes or improvements made based on the teachings of the present invention fall within the protection scope of the present invention.

[0038] Example

[0039] Unless otherwise specified, the materials and instruments used in the following examples were obtained from commercial channels; the detection methods used were all existing methods unless otherwise specified.

[0040] Embodiment 1 White hair moss planting method

[0041] Step (1) was performed under constant light (40 μmol·m -2 ·s -1), under the incubator environment conditions of 80% relative humidity and 21°C, the leaves of the narrow-leaved white-hair moss collected by the supplier: Gao Bin in the wild environment in Jiangyin County, Ankang City, Shaanxi Province in June 2024 were dried in the shade and placed in Knop's medium (potassium nitrate (KNO3): 1.0 g / L, potassium dihydrogen phosphate (KH2PO4): 0.25 g / L, magnesium sulfate (MgSO4·7H2O): 0.25 g / L, calcium chloride (Ca Cl2·2H2O): 0.12g / L, Boric acid (H3BO3): 0.014g / L, Manganese chloride (MnCl2·4H2O): 0.0036g / L, Zinc sulfate (ZnSO4·7H2O): 0.0028g / L, Ferrous sulfate (FeSO4·7H2O): 0.0028g / L, Copper sulfate (CuSO4·5H2O): 0.0003g / L, Ammonium heptamolybdate ((NH4)6Mo7O 24 ·4H2O): 0.0003g / L. Gelatin or agar: 10g / L, adjust pH to 5 with HCl, sterilize and heat-inject the culture dish to obtain the culture medium. ) and culture for 20 days until new shoots are observed to grow;

[0042] Step (2): Add a mixed solution containing 0.8 mg / L abscisic acid, 0.4 mg / L ascorbic acid and 2.0 mg / L ergothioneine to the white hair moss culture medium obtained in step (1), wherein the solvent is water. -2 ·s -1 The culture was carried out under high temperature stress at 30℃ and relative humidity of 80% for 30 days.

[0043] In the second month, high light and high temperature stress screening was carried out. -2 ·s -1 The culture was carried out under high light and high temperature stress at a temperature of 30°C and a relative humidity of 80%, and the stress treatment lasted for 30 days.

[0044] In the third month, high light, high temperature and low humidity stress screening was carried out, and high light and high temperature stress cultivation was carried out under a constant light intensity of 200 μmol·m-2·s-1, a temperature of 30°C and a relative humidity of 60%, and the stress treatment lasted for 30 days.

[0045] During the three-month stress period, the average daily watering rate was 200 mL / (m 2 ·d), spray nutrient solution every 7 days. The nutrient solution is mainly composed of 10 times diluted Knop's nutrient solution, in which 2mmol·L -1 α-peptidase, 10mgL -1 PQQ, 2mgL -1NAA, 0.1% chlorophyll foliar fertilizer, use this nutrient solution to foliar fertilize the white-haired moss, spray 35-45mL of nutrient solution per square decimeter. During the stress period, the white-haired moss that cannot adapt and die should be removed in time, and then the pigment should be measured, and the surviving plants should be selected as stress-resistant plants.

[0046] Step (3): Use scissors to cut off the newly emerged white-haired moss plants from the stress-resistant mother plant, trim the old plants to remove the impurities, and then use a 20% bronopol aqueous solution at an ambient temperature of 18-25°C for detoxification, that is, ultrasonic disinfection for 5 minutes to obtain the detoxified strain for use.

[0047] Step (4): weigh the matrix soil in proportion, and the mass ratio is peat soil (organic matter content is less than 40%): vermiculite: diatomaceous earth, α-ketoglutaric acid calcium ratio is 2:1:1:1. Weigh each raw material and mix them evenly to obtain the matrix soil, spread it on the water surface floating foam board with moss cloth in the water pool in the greenhouse, and disperse the virus-free strains in step (3) on the surface of the matrix soil with an interval of 0.5 cm, and then water it thoroughly. The cultivation site is Kunming, and the environmental conditions in the field are that the ambient temperature in the greenhouse ranges from 15°C to 30°C, the relative humidity ranges from 50% to 90%, and the light intensity of the sunshade net in the greenhouse is less than 100μmol·m-2·s-1, and the white hair moss is propagated in this environment.

[0048] Step (5) Management during the reproduction period: During the reproduction process of white hair moss, the average daily watering amount is 200mL / (m 2 ·d), spray nutrient solution every 7 days. The nutrient solution is mainly composed of 8 times diluted Knop's nutrient solution, in which 2mmol·L -1 α-Ketoglutaric acid, 10mgL -1 Vitamin C, 10mgL -1 GA3, 2mgL -1 NAA, 5mgL-1 brassinolide, foliar fertilization of white hair moss with nutrient solution, 40mL per square decimeter. Dilute the Bacillus subtilis suspension to 1000 times, and then stir it thoroughly to make the bacteria evenly distributed in the liquid. During the cultivation of white hair moss, spray the Bacillus subtilis solution once in the evening on the 15th day of a month, and spray the carbendazim solution once at the end of the month; in addition, the greenhouse is ventilated 2 to 3 times at night every week.

[0049] Step (6): After 4 months of cultivation, the white hair moss continues to expand and grow, and the intervals between the moss branches disappear and become one piece. When it is necessary to divide and transplant the branches again, one cycle of cultivation is completed. The photos of the actual plants in the greenhouse after one cycle of cultivation are as follows Figures 1-2As shown in the figure, it can be seen that a large number of healthy white hair moss can be obtained by adopting the same method of the present application, the planting effect is good, it is suitable for large-scale planting, and the height of a single plant can reach 1.3 cm, and it grows well.

[0050] Example 2

[0051] The difference from Example 1 is that: (1) the collected Holotrichia angustifolia was placed in Knop's medium and kept at a constant light intensity of 38 μmol·m -2 ·s -1 , culturing in an incubator at a relative humidity of 75% and a temperature of 20° C. until new shoots grow, thereby obtaining a culture;

[0052] (2) After adding a mixed solution containing 0.7 mg / L abscisic acid, 0.3 mg / L ascorbic acid, and 1.7 mg / L ergothioneine to the culture, high temperature stress screening and cultivation were carried out for 3 months. During the high temperature stress screening and cultivation period, the average watering amount per day was 170 mL / m 2 ·d. Apply nutrient solution to the leaves every 7 days, spray 35 mL of the first nutrient solution per square decimeter. After the high temperature stress screening and cultivation is completed, the surviving plants are selected as stress-resistant plants;

[0053] (4) Prepare substrate soil and spread it on moss cloth, which is laid on a floating foam board and floated on the surface of a pool in a greenhouse. Virus-free strains are scattered on the surface of the substrate soil at intervals of 0.7 cm in the top, bottom, left and right directions, and watered thoroughly. The ambient temperature in the greenhouse is 15°C, the relative humidity is 90%, and the light intensity under the shade net is <100 μmol·m -2 ·s -1 , the white-haired moss is propagated until the white-haired moss continues to proliferate and grow, and the intervals between the ramets on the moss cloth disappear and become one piece, completing the first cycle of cultivation;

[0054] During the propagation period of white hair moss: the average watering amount for each plant per day is 200mL / (m 2 d) Spray the second nutrient solution once every 7 days at a rate of 35 mL per square decimeter. Spray Bacillus subtilis solution once in the evening on the 15th day of each month and spray carbendazim once at the end of each month. Ventilate the greenhouse twice a week at night.

[0055] The matrix soil used is obtained by mixing peat soil, vermiculite, diatomaceous earth and α-calcium ketoglutarate in a mass ratio of 1.5:1:1:1.

[0056] Example 3

[0057] The difference from Example 1 is that: (1) the collected Holotrichia angustifolia was placed in Knop's medium and kept at a constant light intensity of 42 μmol·m -2 ·s-1 , incubate in an incubator at a relative humidity of 80% and a temperature of 22° C. until new shoots grow, thereby obtaining a culture;

[0058] (2) After adding a mixed solution containing 1.0 mg / L of abscisic acid, 0.5 mg / L of ascorbic acid, and 2.3 mg / L of ergothioneine to the culture, high temperature stress screening and cultivation were carried out for 3 months. During the high temperature stress screening and cultivation period, the average watering amount per day was 220 mL / m 2 ·d. Apply nutrient solution to the leaves every 7 days, spray 45 mL of the first nutrient solution per square decimeter. After the high temperature stress screening and cultivation is completed, the surviving plants are selected as stress-resistant plants;

[0059] (4) Prepare substrate soil and spread it on moss cloth, which is then laid on a floating foam board and floated on the surface of a pool in a greenhouse. Virus-free strains are dispersed on the surface of the substrate soil at intervals of 0.3 cm from top to bottom, left to right, and irrigated thoroughly. The ambient temperature in the greenhouse is 30°C, the relative humidity is 50%, and the light intensity under the shade net is <100 μmol·m -2 ·s -1 , the white-haired moss is propagated until the white-haired moss continues to proliferate and grow, and the intervals between the ramets on the moss cloth disappear and become one piece, completing the first cycle of cultivation;

[0060] During the propagation period of white hair moss: the average watering amount for each plant per day is 200mL / (m 2 d) Spray the second nutrient solution once every 7 days at a rate of 45 mL per square decimeter. Spray Bacillus subtilis solution once in the evening on the 15th day of each month and spray carbendazim once at the end of each month. Ventilate the greenhouse 3 times a week at night.

[0061] The matrix soil used is obtained by mixing peat soil, vermiculite, diatomaceous earth and α-calcium ketoglutarate in a mass ratio of 2.5:1:1:1.

[0062] Comparative Example 1

[0063] The difference from Example 1 is that the cultivation is carried out according to the method in CN202210369820.2. Since this method is cultivated on pine bark stone, it is not easy to collect growth data per unit area, and subsequent measurements cannot be performed.

[0064] 100 white hair mosses were planted by the methods in Examples 1 to 3 and Comparative Examples 1 to 2, and then the proportion of infected bacteria in the obtained plants (the number of white hair mosses infected with miscellaneous bacteria / the number of white hair mosses planted*100%), the yield at harvest (determined by overall weighing), the growth rate (statisticed once a week, recording the growth height of a single white hair moss plant within a week during the whole growth cycle), the color consistency and morphological stability of the harvested products (with the planting sample as a reference, the harvesting personnel recorded the color and morphology of the harvested products one by one, if they were consistent with the planting sample, they were recorded as consistent, and if there were obvious differences, they were recorded as inconsistent), and the results were shown in the following table:

[0065] surface

[0066]

[0067] As can be seen from the above table, the method provided by the present application can effectively maintain the color and morphological characteristics of the mother plants collected in the wild, which is beneficial to maintaining the characteristics of the mother plants. At the same time, it can increase the planting yield and growth rate, reduce the proportion of bacterial infection, and is conducive to large-scale planting in greenhouses.

[0068] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for growing Leucorrhiza angustifolia in an artificial greenhouse, characterized in that: The following steps are involved: (1) The collected white hair moss was placed in Knop's medium under a constant light intensity of 38-42 μmol·m -2 ·s -1 , culturing in an incubator at a relative humidity of 75-80% and a temperature of 20-22° C. until new shoots grow, thereby obtaining a culture; (2) After adding a mixed solution containing 0.7-1.0 mg / L of abscisic acid, 0.3-0.5 mg / L of ascorbic acid, and 1.7-2.3 mg / L of ergothioneine to the culture, high temperature stress screening culture was carried out for 3 months. During the high temperature stress screening culture, the average daily watering amount was 170-220 mL / m 2 ·d. Apply nutrient solution to the leaves every 7 days, spray 35-45 mL of the first nutrient solution per square decimeter. After the high temperature stress screening and cultivation is completed, the surviving plants are selected as stress-resistant plants; (3) cutting off the newly emerged stress-resistant strain, detoxifying it with a bronopol aqueous solution, and then ultrasonically sterilizing it to obtain a virus-free strain; (4) Prepare substrate soil and spread it on moss cloth, which is then laid on a floating foam board and floated on the surface of a pool in a greenhouse. Virus-free strains are dispersed on the surface of the substrate soil at intervals of 0.3 to 0.7 cm above, below, left, and right. Water thoroughly. The ambient temperature in the greenhouse is 15 to 30°C, the relative humidity is 50 to 90%, and the light intensity under the shade net is <100 μmol·m - 2 ·s -1 , the white-haired moss is propagated until the white-haired moss continues to proliferate and grow, and the intervals between the ramets on the moss cloth disappear and become one piece, completing the first cycle of cultivation; During the propagation period of white hair moss: the average watering amount for each plant per day is 200mL / (m 2 ·d) Spray the second nutrient solution once every 7 days, with a spraying rate of 35-45 mL per square decimeter. Spray Bacillus subtilis solution once in the evening on the 15th day of each month, spray carbendazim once at the end of each month, and ventilate the greenhouse 2-3 times at night every week; The matrix soil used is obtained by mixing peat soil, vermiculite, diatomaceous earth and α-calcium ketoglutarate in a mass ratio of 1.5-2.5:1:1:

1.

2. The artificial greenhouse planting method of the angustifolia leucophylla according to claim 1, wherein High temperature stress screening culture includes: In the first month, the light intensity was 78-82 μmol·m -2 ·s -1 , the temperature is 29-32°C, the relative humidity is 78-82%, and the high temperature stress culture is carried out for 30-31 days; In the second month, the light intensity was kept constant at 180-220 μmol·m -2 ·s -1 , high light and high temperature stress culture was carried out at a temperature of 28-32°C and a relative humidity of 78-82% for 30-31 days; In the third month, the light intensity was kept constant at 180-220 μmol·m -2 ·s -1 The culture was carried out under high light, high temperature and low humidity stress at a temperature of 29-32°C and a relative humidity of 58-62%, and the stress treatment lasted for 30-31 days.

3. The artificial greenhouse planting method of the angustifolia leucophylla according to claim 1, wherein The second nutrient solution was composed of 8 times diluted Knop's nutrient solution, to which 2 mmol·L -1 α-Ketoglutaric acid, 10mgL -1 Vitamin C, 10mgL -1 GA3, 2mgL -1 NAA, 5mgL-1 brassinolide.

4. The artificial greenhouse planting method of the angustifolia Leucophylla according to claim 1, wherein The organic matter content of the peat soil used was less than 40%.

5. The artificial greenhouse planting method of the angustifolia leucophylla according to claim 1, wherein The first nutrient solution used was composed of: a 10-fold diluted Knop's nutrient solution, to which 2 mmol·L -1 α-peptidase, 10mgL -1 PQQ, 2mgL - 1 NAA, 0.1% concentration of chlorophyll foliar fertilizer.

6. The artificial greenhouse planting method of Leucophyllum angustifolium according to claim 1, characterized in that: Knop's medium is potassium nitrate: 1.0g / L, potassium dihydrogen phosphate: 0.25g / L, magnesium sulfate: 0.25g / L, calcium chloride: 0.12g / L, boric acid: 0.014g / L, manganese chloride: 0.0036g / L, zinc sulfate: 0.0028g / L, ferrous sulfate: 0.0028g / L, copper sulfate (CuSO4·5H2O): 0.0003g / L, ammonium heptamolybdate: 0.0003g / L. Gelatin or agar: 10g / L, adjust pH to 5 with HCl, sterilize and heat-inject culture dish to obtain culture medium.

7. The artificial greenhouse planting method of Leucorrhiza angustifolia according to claim 1, characterized in that: The Bacillus subtilis suspension is diluted to 800 to 1100 times to obtain a Bacillus subtilis solution.

8. The artificial greenhouse planting method of Leucorrhiza angustifolia according to claim 1, characterized in that: The first cycle of cultivation lasts for 4 months.

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