A bactericidal composition containing cyclobutrifluram and its application

Abstract

The present invention belongs to the technical field of pesticide bactericidal, and discloses a bactericidal composition containing Cyclobutrifluram and its application. The bactericidal composition contains active ingredient A and active ingredient B. The active ingredient A is Cyclobutrifluram, and the active ingredient B is Bronopol. The mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1. The bactericidal composition of the present invention has high activity against various crop diseases, has a significant synergistic effect, can delay the generation of pathogenic bacteria drug resistance, and is beneficial to the comprehensive management of agricultural diseases.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide bactericides, and particularly relates to a bactericidal composition containing Cyclobutrifluram and its application. Background Art

[0002] Cyclobutrifluram is a new nicotinamide bactericidal and nematicidal agent developed by Syngenta. It has a broad control spectrum, is safe and highly effective, can effectively control various nematodes and major fungal diseases. Direct application or seed treatment can effectively control root-knot, sugar beet cyst and corn stunt nematodes on crops such as cucumber, tomato, corn and sugar beet; it can also effectively control plant diseases, especially has excellent control effect on Fusarium. Its CAS registration number: 1460292-16-3. The chemical structure is as follows:

[0003]

[0004] Bronopol is a broad-spectrum bromonitro alcohol bactericide. Bronopol has good control effects on plant fungal and bacterial diseases, especially on bakanae disease of rice, and also has certain control effects on angular leaf spot of cotton and scab of wheat.

[0005] Fusarium fungi are important pathogenic bacteria that cause various soil-borne diseases in crops. In agricultural production, they can cause various plant diseases, such as scab of wheat, basal stalk rot of wheat, bakanae disease of rice and other diseases. The serious occurrence of diseases causes significant economic losses to agricultural production.

[0006] The applicant of the present invention has conducted in-depth research on Cyclobutrifluram and Bronopol, and found that when Cyclobutrifluram and Bronopol are mixed, there is an obvious synergistic effect on scab of wheat, basal stalk rot of wheat and bakanae disease of rice within a certain mixing ratio range, which can effectively improve the control of plant diseases. Through further research, the present invention has been completed. There is no report on the mixture of Cyclobutrifluram and Bronopol for controlling diseases caused by Fusarium fungi. Summary of the Invention

[0007] Based on the above problems, the present invention provides a bactericidal composition which can effectively control plant diseases, has an obvious synergistic effect on the control effects of pathogenic bacteria such as scab of wheat, basal stalk rot of wheat and bakanae disease of rice, can effectively reduce the dosage of pesticides, and is environmentally friendly.

[0008] To achieve the above object, the present invention adopts the following technical solution: A bactericidal composition containing Cyclobutrifluram, wherein the bactericidal composition comprises active ingredient A and active ingredient B, the active ingredient A is Cyclobutrifluram, and the active ingredient B is bronopol;

[0009] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:25 to 35:1;

[0010] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:25, 1:24, 1:21, 1:15, 1:10, 1:5, 1:3, 2:5, 6:5, 10:4, 7:2, 5:1, 17:2, 12:1, 16:1, 24:1, 35:1;

[0011] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:24 to 24:1;

[0012] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:24, 1:21, 1:15, 1:10, 1:5, 1:3, 2:5, 6:5, 10:4, 7:2, 5:1, 17:2, 12:1, 16:1, 24:1;

[0013] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:21 to 24:1;

[0014] Further, the mass ratio of Cyclobutrifluram to bronopol is 1:21, 1:15, 1:10, 1:5, 1:3, 2:5, 6:5, 10:4, 7:2, 5:1, 17:2, 12:1, 16:1, 24:1;

[0015] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of Cyclobutrifluram and bronopol in the bactericidal composition is 1 to 90 wt%;

[0016] Still further, the sum of the contents of Cyclobutrifluram and bronopol in the bactericidal composition is 5 to 80 wt%.

[0017] Furthermore, the bactericidal composition contains, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers;

[0018] The wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, sophora flavescens powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0019] The dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0020] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0021] The thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0022] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0023] The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0024] The defoaming agent is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0025] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivative and water; and / or

[0026] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon and 1,2-benzisothiazolin-3-one; and / or

[0027] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica white, talc powder, montmorillonite and starch; and / or

[0028] The synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0029] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0030] By optimizing the contents of the active ingredients and adjuvants in the pesticide composition, the present invention enables a better balance between toxicity and residue, can enhance the drug effect, reduce the dosage of the drug and lower the cost.

[0031] Furthermore, the dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0032] The solid preparations include powder, granule, sphere, tablet, strip, wettable powder, oil-dispersible powder, milk powder, water-dispersible granule, milk granule, water-dispersible tablet, soluble powder, soluble tablet or soluble granule;

[0033] The liquid preparations include soluble solution, soluble sol, oil agent, film-forming oil agent, emulsifiable concentrate, latex, dispersible liquid, paste, water emulsion, oil emulsion, microemulsion, fat agent, suspension, microcapsule suspension, oil suspension, dispersible oil suspension, suspension emulsion, microcapsule suspension-suspension, microcapsule suspension-water emulsion or microcapsule suspension-suspension emulsion;

[0034] Furthermore, the powder is a free-flowing powdery preparation suitable for dusting or spreading containing active ingredients;

[0035] Furthermore, the granule is a free-flowing granular preparation containing active ingredients with a certain particle size range;

[0036] Furthermore, the sphere is a spherical preparation containing active ingredients;

[0037] Further, the tablet is a sheet preparation containing an active ingredient with a certain shape and size;

[0038] Further, the strip preparation is a strip or rod preparation containing an active ingredient;

[0039] Further, the wettable powder is a powdery preparation in which the active ingredient is dispersed into a suspension in water;

[0040] Further, the oil-dispersible powder is a powdery preparation in which the active ingredient is dispersed into a suspension in an organic solvent;

[0041] Further, the emulsifiable powder is a powdery preparation in which the active ingredient is dissolved in an organic solvent and encapsulated in a soluble or insoluble inert ingredient, and forms an oil-in-water emulsion when dispersed in water;

[0042] Further, the water-dispersible granule is a granular preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0043] Further, the emulsifiable granule is a granular preparation in which the active ingredient is dissolved in an organic solvent and encapsulated in a soluble or insoluble inert ingredient, and forms an oil-in-water emulsion when dispersed in water;

[0044] Further, the water-dispersible tablet is a sheet preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0045] Further, the soluble powder is a powdery preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0046] Further, the soluble granule is a granular preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0047] Further, the soluble tablet is a sheet preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0048] Further, the soluble solution is a liquid preparation that is diluted with water into a transparent or semi-transparent liquid containing an active ingredient and may contain inert ingredients insoluble in water;

[0049] Further, the soluble sol is a colloidal preparation in which the active ingredient forms a true solution when diluted with water;

[0050] Further, the oil preparation is a liquid preparation that is diluted (or not diluted) with an organic solvent into a homogeneous liquid containing an active ingredient;

[0051] Further, the spreading film oil preparation is an oil preparation that automatically spreads into an oil film on the water surface and contains an active ingredient;

[0052] Further, the emulsifiable concentrate is a homogeneous liquid preparation containing an active ingredient, which is diluted and dispersed with water into an emulsion.

[0053] Further, the latex is a latex preparation containing an active ingredient, which is diluted and dispersed with water into an emulsion.

[0054] Further, the dispersible concentrate is a homogeneous liquid preparation containing an active ingredient, which is diluted and dispersed with water into a suspension.

[0055] Further, the ointment is a water-based paste preparation containing an active ingredient that can form a film, and is generally used directly.

[0056] Further, the aqueous emulsion is a liquid preparation in which an active ingredient (or its organic solution) forms an emulsion in water.

[0057] Further, the oil emulsion is a liquid preparation in which an active ingredient (or its aqueous solution) forms an emulsion in oil.

[0058] Further, the microemulsion is a transparent or semi-transparent microemulsion liquid preparation in which an active ingredient is in water, and is used directly or after dilution with water.

[0059] Further, the liniment is an oily or fatty-based viscous preparation containing an active ingredient, and is generally used directly.

[0060] Further, the suspending agent is a stable suspension liquid preparation in which an active ingredient is dispersed as solid particles in water, and is generally diluted with water before use.

[0061] Further, the microcapsule suspending agent is a stable suspension liquid preparation in which microcapsules containing an active ingredient are dispersed in a liquid.

[0062] Further, the oil suspending agent is a stable suspension liquid preparation in which an active ingredient is dispersed as solid particles in a liquid, and is generally diluted with an organic solvent before use.

[0063] Further, the dispersible oil suspending agent is a stable suspension liquid preparation in which an active ingredient is dispersed as solid particles in a non-aqueous medium, and is generally diluted with water before use.

[0064] Further, the suspo-emulsion is a heterogeneous liquid preparation in which an active ingredient is stably dispersed in a continuous aqueous phase in the form of solid particles and tiny water-insoluble droplets.

[0065] Further, the microcapsule suspension-suspending agent is a stable suspension liquid preparation in which an active ingredient is dispersed as microcapsules and solid particles in water.

[0066] Further, the microcapsule suspension-aqueous emulsion is a heterogeneous liquid preparation in which an active ingredient is stably dispersed in a continuous aqueous phase in the form of microcapsules and tiny droplets.

[0067] Further, the microcapsule suspension-suspension emulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in the continuous aqueous phase in the forms of microcapsules, solid particles and tiny droplets;

[0068] Further, the bactericidal composition can be prepared into a pesticide-acceptable preparation dosage form, and the preparation dosage form is a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspension emulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, a seed treatment dry powder;

[0069] Further, the preparation dosage form is a microemulsion, an emulsifiable concentrate, a suspension, an aqueous emulsion, a water-dispersible granule, a seed treatment suspension.

[0070] The present invention also discloses the application of a bactericidal composition containing Cyclobutrifluram as described above in preventing and controlling plant diseases;

[0071] Further, the plant diseases are plant diseases caused by fungi or bacteria;

[0072] Further, the plant diseases are plant diseases caused by fungi;

[0073] Further, the plant diseases caused by fungi are Fusarium head blight of wheat and bakanae disease of rice.

[0074] The beneficial effects of the present invention are as follows:

[0075] 1) The active ingredient action mechanisms of the bactericidal composition of the present invention are different, and it has an obvious effect on delaying the development of pathogen resistance;

[0076] 2) The bactericidal composition of the invention is safe and efficient, safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring harvest. Detailed implementation manners

[0077] In order to make the purpose, advantages and technical solutions of the present invention clearer, the following preparation examples and specific examples of the present invention are used to explain the technical solutions of the present invention. However, the protection scope of the present invention should not be limited by the specific implementation manners described herein.

[0078] Preparation examples of preparations:

[0079] Preparation example 1:

[0080] 28% Cyclobutrifluram·bronopol seed treatment suspension (8:20)

[0081] Formulation composition: Cyclobutrifluram 8%, bronopol 20%, sodium lignosulfonate 2.5%, isomeric tridecanol polyoxyethylene ether 2%, styrylphenol polyoxyethylene ether 2%, polyacrylate emulsion 1%, xanthan gum 0.2%, rose red pigment 4.2%, ethylene glycol 5%, magnesium aluminum silicate 0.5%, silicone defoamer 0.4%, sodium benzoate 1.2%, deionized water to make up the balance;

[0082] Preparation method: According to the ratio, the active ingredients, auxiliaries and water are mixed evenly by high-shear mixing and stirring, and then sanded for 2.5 h by a sand mill to make the average particle size reach 1-5 microns, and the seed treatment suspension can be obtained.

[0083] Preparation Example 2:

[0084] 20% Cyclobutrifluram·bronopol suspension (10:10)

[0085] Formulation composition: Cyclobutrifluram 10%, bronopol 10%, dioctyl sulfosuccinate 3%, castor oil polyoxyethylene ether 1.2%, EO-PO block copolymer 2%, sodium alkylphenol polyoxyethylene ether methyl ether condensate sulfate 3%, polycarboxylate sodium 1.5%, silicone defoamer 1%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 4%, sodium benzoate 1%, deionized water to make up the balance;

[0086] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional auxiliaries are placed in the reaction kettle in turn, mixed with water evenly, and then obtained the suspension product through high-speed shearing and wet sanding, and finally homogenized and filtered.

[0087] Preparation Example 3:

[0088] 44% Cyclobutrifluram·bronopol water dispersible granule (24:20)

[0089] Formulation composition: Cyclobutriflura 24%, bronopol 20%, sodium dodecyl sulfate 8%, naphthalene sulfonate formaldehyde condensate 8%, polycarboxylate sodium 2%, organic expanded clay 5%, kaolin to make up the balance;

[0090] Preparation method: According to the formula ratio of the example, the active ingredients are added to the carrier, and surfactants and other functional auxiliaries are added thereto, mixed, and 10-25% of water is added after air flow pulverization, and then obtained the water dispersible granule product through kneading, granulation, drying and screening; or the pulverized powder is sprayed with water, granulated and dried in a fluidized bed granulator, and then screened to obtain the product.

[0091] Preparation Example 4:

[0092] 10% Cyclobutrifluram·Bronopol Emulsion in Water (5:5)

[0093] Formulation composition: Cyclobutrifluram 5%, Bronopol 5%, Glyceryl fatty acid ester polyoxyethylene ether 3%, Polyoxyethylene sorbitan monooleate 3%, Cyclohexanone 15%, Xanthan gum 0.1%, Glycerol 5%, Urea 1.5%, Sodium sorbate 0.2%, Organosilicon defoamer 0.5%, Deionized water to make up the balance;

[0094] Preparation method: According to the formulation ratio of the examples, dissolve the active ingredients in the solvent and add emulsifiers to form a homogeneous oil phase. Mix deionized water, antifreeze, etc. together to form a homogeneous aqueous phase; under high-speed shearing, add the aqueous phase to the oil phase to form a well-dispersed emulsion in water product.

[0095] Indoor toxicity

[0096] Test agents: 90% Cyclobutrifluram technical material, 95% Bronopol technical material; Other reagents: Solvent acetone (analytical grade), Emulsifier Tween 80 (chemical grade); All are provided by the group R & D center.

[0097] Example 1:

[0098] Indoor activity test for wheat head blight

[0099] Test basis: The test refers to the agricultural industry standard of the People's Republic of China NY / T 1156.2 - 2006 "Pesticide Bioassay Guidelines for Indoor Fungicides Part 2: Inhibiting the Mycelial Growth of Pathogenic Fungi Petri Dish Method".

[0100] Test target: Fusarium pseudograminearum

[0101] Instrument and equipment: Moist heat sterilizer, Laminar flow hood, Constant temperature light incubator, Drying oven, Electronic balance, Pipette, Alcohol lamp, Small beaker, Volumetric flask, Erlenmeyer flask, Petri dish (Φ9cm), Borer, Inoculator (Φ0.6cm), Ruler, etc.

[0102] Agent preparation: Dissolve the above technical materials in acetone respectively, and then dilute with 0.1% Tween 80 aqueous solution to prepare single-agent mother liquors respectively. According to the mixing purpose and agent activity, design different ratios, and configure each single agent and each group of mixed agents into the required series of mass concentrations.

[0103] Test repetition: For each concentration of the test agents, there are 4 Petri dishes, 1 Petri dish for each repetition, with a total of 4 repetitions. Use 0.1% Tween 80 aqueous solution without agents as the blank control.

[0104] Agent treatment: Under aseptic operation conditions, quantitatively add the pre-melted and sterilized PDA medium into a sterile conical flask according to the test treatment. Sequentially and quantitatively pipette 10 mL of each prepared treatment solution from low concentration to high concentration, and add them into the above conical flask respectively. Shake well, and then pour an equal amount into a petri dish to make a drug-containing plate with the corresponding concentration. Set a 0.1% Tween 80 aqueous solution without adding the agent as a blank control, with 4 replicates for each treatment.

[0105] Inoculation: For the pre-cultured Fusarium pseudograminearum, under aseptic conditions, use a sterilized borer to cut a fungal plug from the edge of the colony, and inoculate the fungal plug in the center of the drug-containing plate with an inoculator. Cover the lid and place it in a constant temperature light incubator at 26 °C for dark culture.

[0106] Data investigation: Investigate the growth of the pathogenic fungus hyphae according to the growth of the hyphae in the blank control petri dish. Measure the colony diameter with a ruler, in centimeters (cm). Measure the diameter of each colony once with the cross method, take the average value, and record the original data of all replicates for each treatment.

[0107] Calculate the mycelial growth inhibition rate according to the following formula, in percentage (%), and retain two decimal places for the calculation result.

[0108] D = D1 - D2

[0109] D —— Colony growth diameter;

[0110] D1 —— Colony diameter;

[0111] D2 —— Fungal plug diameter.

[0112] I = (D0 - D t ) / D0 * 100

[0113] In the formula:

[0114] I —— Mycelial growth inhibition rate;

[0115] D0 —— Colony growth diameter of the blank control;

[0116] D t —— Colony growth diameter of the agent treatment.

[0117] Analyze with the DPS statistical analysis system to obtain the toxicity regression line and EC 50 value, and evaluate the activity of the test agent on the biological test material.

[0118] Sun Yunpei method: Evaluate the synergistic effect of the mixed agents according to the co-toxicity coefficient (CTC). A co-toxicity coefficient CTC ≥ 120 for the mixture indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; 80 < CTC < 120 indicates an additive effect.

[0119] The coefficient (CTC value) is calculated by the following formula:

[0120]

[0121] In the formula:

[0122] ATI—the measured toxicity index of the mixture;

[0123] S—the EC of the standard fungicide 50 , with the unit of milligram per liter (mg / L);

[0124] M—the EC of the mixture 50 , with the unit of milligram per liter (mg / L).

[0125] TTI = TI A *P A +TI B *P B

[0126] In the formula:

[0127] TTI—the theoretical toxicity index of the mixture;

[0128] TI A —the toxicity index of fungicide A;

[0129] P A —the percentage content of fungicide A in the mixture, with the unit of percentage (%);

[0130] TI B —the toxicity index of fungicide B;

[0131] P B —the percentage content of fungicide B in the mixture, with the unit of percentage (%).

[0132]

[0133] In the formula:

[0134] CTC—the co-toxicity coefficient;

[0135] ATI—the measured toxicity index of the mixture;

[0136] TTI—the theoretical toxicity index of the mixture.

[0137] The test results are shown in the following table:

[0138] Indoor bioassay test results of cyclobutrifluram and bronopol against Fusarium head blight of wheat:

[0139] Table 1 results show that the EC of cyclobutrifluram against Fusarium head blight of wheat50 was 4.223 mg / L, and the EC of bronopol against wheat head blight 50 was 6.948 mg / L. The bactericidal compositions of cyclobutrifluram and bronopol at ratios of 1:25 to 35:1 all showed good control effects. The co-toxicity coefficients of the bactericidal compositions of cyclobutrifluram and bronopol at ratios of 1:21 to 24:1 were > 120, all showing synergistic effects. Among them, the co-toxicity coefficient value of cyclobutrifluram and bronopol = 10:4 was the largest, being 229.644, and the EC 50 was 2.071 mg / L, with an obvious synergistic effect.

[0140] Table 1 Determination results of the synergistic effects of different ratios of cyclobutrifluram and bronopol on wheat head blight

[0141] Test agents Regression equation <![CDATA[r 2 > <![CDATA[EC 50 > Coefficient of co-toxicity Effect Biopesticide B value ± standard error / (mg / L) (ATI) (TTI) (CTC) / Cyclobutrifluram (A) 1.100±0.119 0.982 4.223(3.428～5.202) 100.000 / / / Bronopol (B) 1.861±0.152 0.990 6.948(5.887～8.200) 60.780 / / / A:B = 1:25 1.876±0.255 0.973 6.559(5.001～8.604) 64.385 62.289 103.365 Additive effect A:B = 1:21 2.212±0.257 0.980 4.082(3.228～5.162) 103.454 62.563 165.361 Synergistic effect A:B = 1:10 1.926±0.190 0.985 3.831(3.151～4.659) 110.232 64.346 171.313 Synergistic effect A:B = 1:5 2.094±0.223 0.983 3.587(2.913～4.418) 117.731 67.317 174.891 Synergistic effect A:B = 2:5 1.978±0.132 0.993 3.121(2.743～3.552) 135.309 71.986 187.967 Synergistic effect A:B = 10:4 1.886±0.116 0.994 2.071(1.839～2.332) 203.911 88.794 229.644 Synergistic effect A:B = 5:1 2.02±0.140 0.992 3.181(2.783～3.635) 132.757 93.463 142.042 Synergistic effect A:B = 12:1 2.008±0.138 0.993 3.337(2.922～3.811) 126.551 96.983 130.487 Synergistic effect A:B = 24:1 1.926±0.158 0.990 3.518(2.996～4.130) 120.040 98.431 121.953 Synergistic effect A:B = 35:1 2.164±0.196 0.987 3.681(3.081～4.39) 114.724 98.911 115.988 Additive effect

[0142] Note: The above data were analyzed using DPS statistical analysis software, and the values were retained to 3 decimal places. The same applies to Table 2 below.

[0143] Example 2:

[0144] Indoor activity test of rice bakanae disease

[0145] Test basis: The test referred to the agricultural industry standard of the People's Republic of China NY / T 1156.2 - 2006 "Pesticide bioassay in the laboratory - Test guidelines for fungicides - Part 2: Inhibiting the mycelial growth of pathogenic bacteria - Petri dish method".

[0146] Test strain: Fusarium moniliforme, provided by Shenyang Research Institute of Chemical Industry.

[0147] Instrument and equipment: High - pressure steam sterilizer, laminar flow hood, incubator, drying oven, electronic balance, pipette, alcohol lamp, beaker (50 mL), volumetric flask, Erlenmeyer flask (100 mL), Petri dish (Φ9 cm), borer (Φ0.6 cm), inoculator, ruler, etc.

[0148] Culture conditions of the test target: Transfer the Fusarium moniliforme stored at 4°C in the refrigerator indoors to the culture medium, and place it in a 25°C incubator for dark culture for 5 days to activate it for standby.

[0149] Formulation of medicaments: Dissolve the above technical materials with acetone respectively to prepare high - concentration mother liquors, and then dilute them with 0.1% Tween 80 aqueous solution. Prepare single - agent mother liquors respectively, and design different ratios according to the mixing purpose and the activity of the medicaments. Each single agent and the mixed agents of each group of ratios are formulated into the required series of mass concentrations.

[0150] Test repetition: For each concentration of the test agent, 4 petri dishes were used, with 1 petri dish for each repetition, and a total of 4 repetitions were carried out. An aqueous solution of 0.1% Tween 80 without the agent was used as the blank control.

[0151] Agent treatment: Under aseptic operation conditions, use a pipette to add 5 mL of the liquid medicine with different concentrations to the pre-calibrated sterile Erlenmeyer flask respectively. Then add the medium melted and cooled to an appropriate temperature to the Erlenmeyer flask, shake well, and pour an equal amount into 4 petri dishes to make the PDA plates containing the drug at the corresponding concentration.

[0152] Inoculation: Cut the mycelial cake from the edge of the colony of Fusarium moniliforme pre-cultured under aseptic conditions with a sterile borer. Use an inoculator to inoculate the mycelial cake in the center of the drug-containing plate, cover the lid, and place it in a constant temperature incubator at 25 °C for dark culture.

[0153] Data investigation: Conduct the test investigation when the colony in the control treatment grows to 2 / 3 - 4 / 5 of the diameter of the petri dish. Measure the colony diameter (cm) with a ruler, measure the diameter of each colony once using the cross method, and take the average value.

[0154] Calculate the mycelial growth inhibition rate according to the following formula, with the unit of percentage (%), and retain two decimal places for the calculation result.

[0155] D = D1 - D2

[0156] D - - Colony growth diameter;

[0157] D1 - - Colony diameter;

[0158] D2 - - Mycelial cake diameter.

[0159] I = (D0 - D t ) / D0 * 100

[0160] In the formula:

[0161] I - - Mycelial growth inhibition rate;

[0162] D0 - - Colony growth diameter of the blank control;

[0163] D t - - Colony growth diameter of the agent treatment.

[0164] Analyze with the DPS statistical analysis system to obtain the toxicity regression line and EC 50 value, and evaluate the activity of the test agent on the biological test material.

[0165] Sun Yunpei method: Evaluate the synergistic effect of the mixed agents according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the compound is ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0166] The coefficient (CTC value) is calculated by the following formula:

[0167]

[0168] Where:

[0169] ATI—the measured toxicity index of the mixture;

[0170] S—the EC of the standard fungicide 50 , with the unit of milligram per liter (mg / L);

[0171] M—the EC of the mixture 50 , with the unit of milligram per liter (mg / L).

[0172] TTI = TI A *P A +TI B *P B

[0173] Where:

[0174] TTI—the theoretical toxicity index of the mixture;

[0175] TI A —the toxicity index of fungicide A;

[0176] P A —the percentage content of fungicide A in the mixture, with the unit of percentage (%);

[0177] TI B —the toxicity index of fungicide B;

[0178] P B —the percentage content of fungicide B in the mixture, with the unit of percentage (%).

[0179]

[0180] Where:

[0181] CTC—the co-toxicity coefficient;

[0182] ATI—the measured toxicity index of the mixture;

[0183] TTI—the theoretical toxicity index of the mixture.

[0184] Results of the bioassay test on the control of rice bakanae disease by cyclobutrifluram and bronopol:

[0185] Table 2 results show that the EC of cyclobutrifluram for controlling rice bakanae disease 50The EC value of bronopol for controlling rice seedling disease is 7.453 mg / L. 50 The ratio of Cyclobutrifluram to bronopol was 5.346 mg / L. The ratio of Cyclobutrifluram to bronopol in the ratio of 1:24 to 24:1 showed good protection effect, and the co-toxicity coefficient was greater than 120, showing synergistic effect. Among them, the co-toxicity coefficient of Cyclobutrifluram to bronopol in the ratio of 6:5 was the largest, which was 270.229>120, and EC 50 It is 2.339 mg / L, showing a synergistic effect.

[0186] Table 2 The results of the synergistic effect of different ratios of Cyclobutrifluram and bronopol on rice bakanae disease

[0187] Test agents Regression equation <![CDATA[r 2 > <![CDATA[EC 50 > Coefficient of co-toxicity Effect Biopesticide B value ± standard error / (mg / L) (ATI) (TTI) (CTC) / Cyclobutrifluram (A) 1.723±0.100 0.996 7.453(6.784～8.188) 100.000 / / / Bronopol (B) 1.206±0.135 0.987 5.346(4.164～6.864) 139.413 / / / A:B = 1:24 1.577±0.044 0.998 3.677(3.475～3.891) 202.692 137.836 147.053 Synergistic effect A:B = 1:15 1.651±0.077 0.996 3.529(3.208～3.882) 211.193 136.949 154.212 Synergistic effect A:B = 1:10 1.609±0.045 0.998 2.982(2.819～3.155) 249.933 135.830 184.005 Synergistic effect A:B = 1:3 1.631±0.050 0.998 2.772(2.602～2.953) 268.867 129.559 207.524 Synergistic effect A:B = 6:5 1.657±0.066 0.997 2.339(2.159～2.534) 318.640 117.915 270.229 Synergistic effect A:B = 7:2 1.696±0.096 0.995 3.422(3.062～3.824) 217.797 108.758 200.257 Synergistic effect A:B = 16:1 2.142±0.084 0.997 4.358(4.038～4.704) 171.019 102.318 167.144 Synergistic effect A:B = 24:1 1.729±0.039 0.999 5.380(5.146～5.624) 138.532 101.577 136.382 Synergistic effect A:B = 30:1 1.712±0.163 0.986 6.183(5.1435～7.432) 120.599 101.271 119.085 Additive effect

[0188] Embodiment 3:

[0189] Experiment on Controlling Rice Bakanae Disease in Field

[0190] Test basis: The test refers to GB / T 17980.104-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 104: Fungicides for Control of Rice Seedling Disease".

[0191] Test target: Rice seedling pathogen.

[0192] Experimental crops: rice.

[0193] Experimental design: The plot treatments of test drug, control drug and blank control were arranged in random blocks.

[0194] Table 3 Test agents and dosage

[0195]

[0196] The experiment set up four treatments for seed soaking with chemicals, and used clean water as a blank control. The area of each seedbed treatment was 10m 2 The seedbed test was not repeated; the field planting area was 30m 2 Each treatment in the field experiment was repeated 4 times.

[0197] The experiment involved soaking seeds on May 20, 2021, germination on May 24, and sowing on May 27.

[0198] For each treatment, the drug solution was prepared according to the experimental design concentration and fully stirred. Dry seeds were poured into the solution and soaked for 48 hours for germination. During the soaking period, the rice seeds were kept above the water surface. After soaking, the rice was not washed but germinated directly.

[0199] Sow in the seedling trays after 3 days, and strictly control the temperature and humidity of the seedbed at different leaf age stages according to the rice technical regulations. After emergence, move the seedling trays to the seedling field, and then move them to the large field when the seedling age reaches 30 days.

[0200] Investigation items: The germination rate was investigated before sowing, and the disease index was investigated once before transplanting (June 15th) and at the booting stage of paddy rice in the large field (August 20th) respectively.

[0201] Before sowing, investigate the germination rate. Each treatment was repeated 4 times. Randomly select 100 seeds, check the germination situation, and calculate the germination rate.

[0202] Before transplanting in the seedling stage, take 5-point samples in each plot in the seedling field, investigate 200 seedlings at each point, investigate the diseased plant rate, and calculate the control effect.

[0203] At the booting stage in the large field, randomly take 5-point samples in each plot, investigate 20 hills at each point, record the number of diseased plants, and calculate the diseased plant rate and control effect of each treatment.

[0204] Method for calculating drug efficacy:

[0205]

[0206]

[0207]

[0208] The test results are as follows:

[0209] As can be seen from Table 4, the pesticide composition of the present invention shows obvious control effects on rice bakanae disease. Compared with the blank control agent treatment, the emergence rate is basically the same as that of the blank control, indicating that after the seed soaking treatment of rice seeds with each preparation example, there is no phytotoxicity to rice. Compared with the single fungicide, the diseased plant rate of each compound preparation is reduced, and it has a good control effect.

[0210] Table 4 Test results of controlling rice bakanae disease with different treatments

[0211]

[0212]

[0213] Example 4:

[0214] Field efficacy test of wheat scab

[0215] Test site: Conducted in the fields where wheat scab often occurs in Hebei Province

[0216] Test time: At the early heading stage of wheat on May 10, 2020

[0217] Test agents and dosage: See Table 5 for details of dosage.

[0218] Table 5 Comparison table of dosage of field test

[0219] Treatment Agent <![CDATA[Dosage g a.i / hm 2 > A1 20% Cyclobutrifluram·Bronopol suspension concentrate (10:10) 40 A2 20% Bronopol water dispersible granules 150 A3 20% Cyclobutrifluram suspension concentrate 50 A4 Water control /

[0220] Experimental treatment: single-dose and water control were set, and the interval between the two applications was 10 days. The spraying time was the early heading stage and the flowering stage of wheat. The area of each treatment in the plot test was 667m 2 , no duplication. Use conventional electric sprayer for spraying.

[0221] Survey method: 5-point sampling and fixed-point survey. Survey 1m per point per treatment. 2 , investigate the total number of ears and the number of diseased ears 10 days before harvest. The severity of the disease is divided into 5 levels according to the disease status of wheat ears:

[0222] Level 0, no disease;

[0223] Level 1: the number of diseased wheat ears accounts for less than 1 / 4 of all wheat ears;

[0224] Level 2: the number of diseased wheat ears accounts for 1 / 4 to 1 / 2 of all wheat ears;

[0225] Level 3: the number of diseased wheat ears accounts for 1 / 2 to 3 / 4 of all wheat ears;

[0226] Level 4: The number of diseased wheat ears accounts for more than 3 / 4 of all wheat ears.

[0227] Calculation formula:

[0228] Disease index:

[0229] Disease index = ∑ (number of diseased plants at each level × disease level) / (total number of plants surveyed × maximum level)

[0230] Prevention effect:

[0231] Control effect = (control disease index - treatment disease index) / control disease index

[0232] And calculate according to the unit area 1m 2 Average ear number and diseased ear rate; wheat samples were collected at harvest time and the 667m2 of each plot was measured after drying for 3 days. 2 Measured output.

[0233] Data were processed using Microsoft Excel 2003, and DPS data were used for statistical analysis and difference significance test (Duncan method).

[0234] Table 6 results showed that through field efficacy plot trials, the survey results 10 days before harvest showed that the control effect of 20% Cyclobutrifluram·bronopol suspension concentrate (10:10) against Fusarium head blight of wheat was 96.12%. At the 0.01 and 0.05 levels, compared with the control single agents 20% bronopol water dispersible granules and 24% Cyclobutrifluram suspension concentrate, the mixed formulation was significantly higher than the control single agents.

[0235] Table 6 Control of Fusarium head blight of wheat by different compound formulations and single agents

[0236]

[0237] Note: The data of each treatment above were calculated according to the unit area of 1 m 2 Average spike number per plant and diseased spike rate; the control effect % in the above table was the average value of each repetition; lowercase letters represented significant differences at the 5% level, and uppercase letters represented significant differences at the 1% level.

[0238] Table 7 Effects of different pesticides on controlling Fusarium head blight of wheat on wheat yield

[0239] Treatment <![CDATA[Yield / kg / 667m 2 > Difference Safety 20% Cyclobutrifluram·Bronopol suspension concentrate (10:10) 485.26 a Normal 20% Bronopol water dispersible granules 473.48 a Normal 24% Cyclobutrifluram suspension concentrate 467.29 a Normal Water control 371.51 b Normal

[0240] Note: The yield in the above table was the average value of each repetition; lowercase letters represented significant differences at the 5% level.

[0241] The results of field efficacy plot trials showed that the compounding of Cyclobutrifluram and bronopol not only reduced the diseased spike rate and disease index during the control of Fusarium head blight of wheat, had a significant control effect, but also could increase the yield of wheat, showing an obvious yield increase effect. In addition, according to the observations after application, the wheat growth in each treatment area was normal and there was no phytotoxicity, indicating that each pesticide was safe for wheat growth at the dosage used in this experiment.

[0242] In summary, through indoor toxicity determination and field efficacy trials, it can be seen that the pesticide composition of the present invention has good control effects on Fusarium head blight of wheat and bakanae disease of rice, is safe for target crops, has a significant control effect, is superior to single agents in delaying the generation of drug resistance and prolonging the persistence, can effectively reduce costs and reduce pesticide residues.

[0243] Although this application has described specific embodiments in detail with the help of examples, the disclosure of this application can adopt various modifications and replacement forms. However, it should be understood that the disclosure of this application is not limited to the specific forms disclosed. On the contrary, the disclosure of this application covers all modifications, equivalents and replacement forms within the scope of the disclosure of this application, and the scope of this application is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition containing Cyclobutrifluram, characterized in that, The described bactericidal composition contains active ingredient A and active ingredient B. The active ingredient A is Cyclobutrifluram, and the active ingredient B is bronopol. The mass ratio of Cyclobutrifluram to bronopol is 1:24 to 24:

1.

2. The bactericidal composition according to claim 1, wherein The mass ratio of Cyclobutrifluram to bronopol is 1:21 to 24:

1.

3. The bactericidal composition according to claim 1, wherein Based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of Cyclobutrifluram and bronopol in the bactericidal composition is 1 to 90 wt%.

4. The bactericidal composition according to claim 1, characterized in that, Based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of Cyclobutrifluram and bronopol in the bactericidal composition is 5 to 80 wt%.

5. The bactericidal composition according to claim 1, characterized in that, The described bactericidal composition contains, in addition to the active ingredients, agriculturally acceptable auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

6. The bactericidal composition according to claim 5, wherein The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

7. The bactericidal composition according to claim 6, characterized in that, The dosage form of the bactericidal composition is microemulsion, water emulsion, suspension, dispersible oil suspension, soluble solution, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water dispersible granule, wettable powder, granule, seed treatment suspension, seed treatment dry powder.

8. The bactericidal composition according to claim 7, wherein The dosage form of the bactericidal composition is microemulsion, emulsifiable concentrate, suspension, water emulsion, water dispersible granule, seed treatment suspension.

9. Use of the bactericidal composition according to any one of claims 1-8 in the prevention and control of plant diseases, characterized in that, The described plant diseases are wheat scab and rice bakanae disease caused by fungi.

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