Visual electrophysiological signal detection device for small aquatic animals

Abstract

The invention relates to a small aquatic animal visual electrophysiological signal detection device which comprises a signal processing system and a signal acquisition assembly, the signal acquisition assembly comprises a first electrode, a second electrode, a third electrode and a conductive platform, one end of the first electrode is communicated with the signal processing system, and the other end of the first electrode is communicated with the conductive platform; one end of the second electrode is communicated with the signal processing system, the other end of the second electrode is communicated with the conductive platform, one end of the third electrode is communicated with the signal processing system, and the other end of the third electrode is communicated with the second electrode. Through the first, second and third combined electrode forms and the combined innovative design of the acting electrode and the conductive platform, the detection (object) range is expanded, the detection is not limited by the prior art any more, the detection precision is improved, accurate detection of visual electrophysiological signals of any small fish becomes possible, and the detection efficiency is improved. Conditions are created for formation of detection standards of visual electrophysiological signals of small fish animals.

Description

Technical Field

[0001] The invention belongs to the technical field of electrophysiological detection, and in particular relates to a device for detecting visual electrophysiological signals of small aquatic animals. Background Art

[0002] It is a very important detection method to assist in determining eye-related diseases through retinal electrophysiological signals. The actual correlation between visual electrophysiological signals and related diseases in the changing process of the disease cycle, as well as how electrophysiological signals evolve under the influence of multiple variables in multiple diseases, require the use of visual electrophysiological models to conduct corresponding damage experiments in order to be fully verified. At present, the visual electrophysiological models mainly consider rats, mice, guinea pigs, rabbits, and fish. The research on rats, mice, guinea pigs, and rabbits is quite common and relatively mature, but the research on fish, such as amphioxus, platypus, swordtail fish, and zebrafish is relatively small. Through the continuous efforts of scientists, the research on fish has made significant progress in recent years. For example, for zebrafish, there are currently about 20 types of zebrafish strains that have matured cell labeling technology, tissue transplantation technology, mutation technology, haploid species technology, gene recombination technology, gene activity inhibition technology, etc. The excellent resources of the molecular mechanism of embryonic development under study have become one of the important models of vertebrate developmental biology.

[0003] Although research on fish is in full swing, the main research fish species, such as platypus, swordtail and zebrafish, are all small aquatic animals with small bodies, making it difficult to detect retinal electrophysiological signals. Currently, there is no unified technical standard in China for detecting visual electrophysiological signals of small fish. Various measurement methods available on the market at home and abroad are mainly aimed at patients and terrestrial animals.

[0004] Due to the existence of the above-mentioned problems, different detection methods may lead to different detection results, or even wrong results. Uncertain detection methods may lead to different results in the same situation. This lack of definite detection standards may cause the right and wrong to lose direction. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a device for detecting visual electrophysiological signals of small aquatic animals, which can accurately capture the visual electrophysiological signals of small animals and can adopt the same method to carry out repeated experiments, thereby providing the possibility for formulating detection standards and solving the problem that the existing technology cannot accurately obtain the visual electrophysiological signals of small animals and the detection results are unreliable.

[0006] The objective of the present invention is achieved through the following technical solutions:

[0007] A device for detecting visual electrophysiological signals of small aquatic animals comprises a signal processing system and a signal acquisition component, wherein the signal acquisition component comprises a first electrode, a second electrode, a third electrode and a conductive platform, wherein the small animal is placed on the conductive platform, wherein one end of the first electrode is connected to the signal processing system, and the other end is electrically connected to the animal through an action electrode, wherein one end of the second electrode is connected to the signal processing system, and the other end is connected to the conductive platform, wherein one end of the third electrode is connected to the signal processing system, and the other end is connected to the second electrode, wherein the action electrode comprises a silver needle electrode and a flexible needle body arranged at the end of the silver needle electrode, wherein the flexible needle body is a cone-shaped body, wherein the silver needle electrode is inserted from the bottom of the cone-shaped body, and the top of the cone-shaped body contacts the cornea of ​​the animal, wherein the flexible needle body is infiltrated with a culture medium, wherein visual electrophysiological signals are acquired from the cornea of ​​the animal and transmitted to the silver needle electrode, and then transmitted to the first electrode through the silver needle electrode, and then processed and displayed by the signal processing system, and wherein the second electrode is inserted into an area 1-2 cm away from the tail of the small animal.

[0008] Furthermore, the conductive platform includes a support plate and a flexible conductive plate arranged on the support plate, and the small animal is placed on the flexible conductive plate.

[0009] Furthermore, the flexible conductive plate and the flexible needle body are made of the same material.

[0010] Furthermore, the flexible conductive plate and the flexible needle body are made of sponge, linen or cotton yarn.

[0011] Furthermore, a limiting column for fixing the electrode wire is arranged on the support plate, a magnetic suction cup for fixing to the support plate is arranged at the bottom of the limiting column, and a fixing hole for the electrode wire to pass through is arranged at the top of the limiting column.

[0012] Furthermore, the signal processing system is provided with a plurality of connection holes for inserting and connecting the first electrode and the second electrode.

[0013] The beneficial effects of the present invention are:

[0014] On the one hand, the present invention sets the active end of the first electrode as a cone-shaped flexible needle body, the cone tip of the flexible needle body can easily form contact with the cornea of ​​the animal or its adjacent conjunctiva, and the bottom of the flexible needle body has a stabilizing effect, making the contact stable and reliable. When the flexible needle body is soaked with a culture medium, the flexible needle body has the ability to conduct electricity and conduction. This improvement greatly improves the ability to stably obtain corneal signals and is suitable for any small animal; on the other hand, the present invention also improves the accuracy of detection through the combination mode of the first electrode, the second electrode, and the third electrode. The first electrode is a positive electrode, which is placed on the cornea or its adjacent conjunctiva to record visual electrophysiological signals. The second electrode is a negative electrode, which is placed in the adjacent area of ​​the animal to provide an electrode for a reference point or a reference point. The main function is to provide a comparison benchmark so that the experimenter can observe the changes in the time of the EEG signal of interest. The position selection of the reference electrode can affect the interpretation of the EEG signal and the results of data analysis; the third electrode is a grounding electrode, which is used to provide a circuit location to ensure the stability and accuracy of the system's electrical signals. The main function is to provide a stable potential reference to eliminate noise from environmental interference, power frequency interference, and human movement. In the present invention, the third electrode is connected to the second electrode to form an integrated structure, and the action point of the second electrode can be used as the action point of the third electrode, thereby eliminating the need for a third connection.

[0015] The reference electrode and the ground electrode of the present invention are designed as an integrated whole. The selection of the placement position and the signal stability are extremely important for recording the visual electrophysiological signals of small animals. After continuous attempts, it is found that the best position for the electrode to be placed 1 cm away from the tail of the small animal is the best position, and the visual electrophysiological signal is relatively stable.

[0016] In summary, the present invention expands the detection (object) range through the innovative design of the first, second, and third combined electrode forms and the combination of the active electrode and the conductive platform, is no longer limited by the existing technology, improves the accuracy of detection, and provides the possibility for accurate detection of visual electrophysiological signals of any small fish and animals, creating conditions for the formation of detection standards for visual electrophysiological signals of small fish and animals.

[0017] Other advantages, objectives and features of the present invention will be described in the following description to some extent, and to some extent, will be obvious to those skilled in the art based on the following examination and study, or can be guided by the practice of the present invention. The objectives and other advantages of the present invention can be realized and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention will be further described in detail below with reference to the accompanying drawings, in which:

[0019] Figure 1is a schematic diagram of the present invention;

[0020] Figure 2 is a schematic diagram of an active electrode of the present invention;

[0021] Figure 3 for Figure 2 Right view of .

[0022] Description of reference numerals:

[0023] 1-Signal processing system; 2-Signal acquisition component; 3-First electrode; 4-Second electrode; 5-Third electrode; 6-Conductive platform; 7-Display; 8-Active electrode; 9-Silver needle electrode; 10-Flexible needle body; 11-Small animal; 12-Workbench; 13-PVA sponge layer; 14-Electrode connection hole; 15-Limiting column. DETAILED DESCRIPTION

[0024] The preferred embodiments of the invention will be described in detail below with reference to the accompanying drawings. It should be understood that the preferred embodiments are only for illustrating the invention, rather than for limiting the protection scope of the invention.

[0025] like Figure 1-3As shown, a device for detecting visual electrophysiological signals of small aquatic animals of the present invention comprises a signal processing system 1 and a signal acquisition component 2. The signal processing system refers to the visual electrophysiological medical equipment currently available on the market. Visual electrophysiological supporting software is installed on the computer of the device to perform corresponding processing on the visual electrophysiological signals. At least a display 7 is provided to display the processing results. The signal acquisition component includes a first electrode 3, a second electrode 4, a third electrode 5 and a conductive platform 6. A small animal 11 is placed on the conductive platform. One end of the first electrode is adapted and connected to the electrode connection hole 14 on the workbench of the equipment, and the other end is electrically connected to the animal through the working electrode to realize the collection of the animal's corneal signal. One end of the second electrode is connected to the signal processing system, and the other end is connected to the conductive platform, so that the receiving end constitutes a reference. One end of the third electrode is connected to the signal processing system, and the other end is connected to the second electrode. The working electrode 8 includes a 1.1 cm long silver needle electrode 9 and a flexible needle body 10 arranged at the end of the silver needle electrode. The shape of the flexible needle body is a cone, the small end is used to contact the animal, and the large end is used to stably connect with the needle. A specific embodiment is to use a PVA sponge to trim a cone with a bottom length of 5 mm and a height of 1.7 cm. It can be a triangular pyramid or a round cone. The cone, the silver needle electrode is inserted from the bottom of the cone, the top of the cone contacts the animal cornea, the flexible needle body is infiltrated with culture medium, the visual electrophysiological signal is obtained from the animal cornea and transmitted to the silver needle electrode, and then transmitted to the first electrode through the silver needle electrode, and then processed and displayed by the signal processing system, the second electrode and the third electrode are specially designed integrated three-terminal electrodes, wherein the two ends are respectively connected to the reference electrode hole and the ground electrode hole on the device, and the other end is a 2 cm long needle electrode, which is inserted into the conductive platform in the area 1-2 cm away from the tail of the small animal. The conductive platform of this embodiment includes the workbench 12 of the device itself, and then a PVA sponge thin layer 13 is arranged on the top, which can be a square plate with a length of 4.5 cm, a width of 3 cm, and a height of 1 cm, as a moisturizing platform for placing small animals such as zebrafish, etc. Before use, the moisturizing platform and the flexible needle body need to be soaked with culture medium (2.5 g / L Soak in NaCl, 0.25g / L CaCl, 0.25g / L KCl, and 0.25g / L NaHCO3 for 2-5 minutes, take it out and place it for 2-5 minutes for use. At this time, the moisturizing platform and the flexible needle body have the ability to conduct electricity and can transmit signals. Dilute the zebrafish anesthetic with culture medium, then place the zebrafish in the anesthetic solution, and perform the detection operation after it is completely anesthetized.

[0026] The first electrode of this embodiment is used to record visual electrophysiological signals, the second electrode is used to provide a comparison reference, and the third electrode is used to provide a location for the circuit. The three form a stable loop through a conductive platform. Through the combined means of precise extraction, stable comparison and stable grounding, the entire process of electrical signal extraction is controlled as a whole, which significantly improves the accuracy of detection and provides the possibility for the establishment of detection standards.

[0027] In this embodiment, the flexible conductive plate and the flexible needle body may also be made of materials such as linen or cotton yarn.

[0028] In this embodiment, multiple groups of connection holes are provided for inserting and connecting the first electrode and the second electrode.

[0029] In this embodiment, a limiting column 15 for fixing the electrode wire is provided on the workbench, a magnetic sucker for fixing with the support plate is provided at the bottom of the limiting column, and a fixing hole for the electrode wire to pass through is provided at the top of the limiting column. The limiting column can be quickly adsorbed and fixed on the workbench through the magnetic sucker, and the electrode can be better limited through the fixing hole, so that the electrode wire will not be pulled or pulled during use, and the electrical signal is free from interference.

[0030] Since the limiting column adopts a magnetic attraction method, its position on the workbench does not need to be fixed, and its position can be adjusted at any time as needed, so as to better adapt to the orientation of the electrode line and achieve a better limiting effect.

[0031] In this embodiment, it is necessary to provide multiple groups of connection holes in the signal processing system, and arrange them in two rows, the left one is usually connected to the reference electrode, the right one is connected to the working electrode, and the lower left one is connected to the ground electrode.

[0032] In actual use, after adjusting parameters such as time and magnification, the test can be carried out through a fixed set of connection holes to achieve a unified benchmark, without large changes in the measured values, saving adjustment time and improving operational efficiency.

[0033] Normally, small animals are first dark-adapted for about 12 hours, and then signals are recorded under different light stimulation intensities (the intensity must increase from small to large, for example, first record 0.01HZ, repeat three times, then record 0.1HZ...). At this time, a flash stimulator is needed to emit stimulation light of different frequencies to obtain corneal physiological signals under different light intensities.

[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution, which should be included in the scope of the claims of the present invention.

Claims

1. A device for detecting visual electrophysiological signals of small aquatic animals, comprising a signal processing system and a signal acquisition component, characterized in that: The signal acquisition component includes a first electrode, a second electrode, a third electrode and a conductive platform. The small animal is placed on the conductive platform. One end of the first electrode is connected to the signal processing system, and the other end is electrically connected to the animal cornea through the active electrode. One end of the second electrode is connected to the signal processing system, and the other end is connected to the conductive platform. One end of the third electrode is connected to the signal processing system, and the other end is connected to the second electrode. The active electrode includes a silver needle electrode and a flexible needle body arranged at the end of the silver needle electrode. The flexible needle body is a cone. The silver needle electrode is inserted from the bottom of the cone, and the top of the cone contacts the animal cornea. The flexible needle body is infiltrated with a culture medium. Visual electrophysiological signals are acquired from the animal cornea and transmitted to the silver needle electrode, and then transmitted to the first electrode through the silver needle electrode, and then processed and displayed by the signal processing system. The second electrode is inserted into an area 1-2 cm away from the tail of the small animal.

2. The device and method for detecting visual electrophysiological signals of small aquatic animals according to claim 1, characterized in that: The conductive platform comprises a support plate and a flexible conductive plate arranged on the support plate, and the small animal is placed on the flexible conductive plate.

3. The device and method for detecting visual electrophysiological signals of small aquatic animals according to claim 2, characterized in that: The flexible conductive plate and the flexible needle body are made of the same material.

4. The device and method for detecting visual electrophysiological signals of small aquatic animals according to claim 3, characterized in that: The flexible conductive plate and the flexible needle body are made of sponge, linen or cotton yarn.

5. The device and method for detecting visual electrophysiological signals of small aquatic animals according to claim 2, characterized in that: The support plate is provided with a limiting column for fixing the electrode wire, the bottom of the limiting column is provided with a magnetic suction cup for fixing with the support plate, and the top of the limiting column is provided with a fixing hole for the electrode wire to pass through.

6. The device and method for detecting visual electrophysiological signals of small aquatic animals according to any one of claims 1 to 5, characterized in that: The signal processing system is provided with a plurality of connection holes for inserting and connecting the first electrode and the second electrode.

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